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WO2014004638A3 - Methods and compositions for enhancing gene expression - Google Patents

Methods and compositions for enhancing gene expression Download PDF

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Publication number
WO2014004638A3
WO2014004638A3 PCT/US2013/047837 US2013047837W WO2014004638A3 WO 2014004638 A3 WO2014004638 A3 WO 2014004638A3 US 2013047837 W US2013047837 W US 2013047837W WO 2014004638 A3 WO2014004638 A3 WO 2014004638A3
Authority
WO
WIPO (PCT)
Prior art keywords
methods
compositions
expression
gene expression
organism
Prior art date
Application number
PCT/US2013/047837
Other languages
French (fr)
Other versions
WO2014004638A2 (en
Inventor
Tedd D. Elich
Original Assignee
Monsanto Technology Llc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Monsanto Technology Llc filed Critical Monsanto Technology Llc
Publication of WO2014004638A2 publication Critical patent/WO2014004638A2/en
Publication of WO2014004638A3 publication Critical patent/WO2014004638A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Cell Biology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

Methods for making expression constructs for enhancing gene expression in an organism are provided. The expression constructs comprise a constitutive promoter operably linked to a polynucleotide, which comprises a 5'-untranslated region (5'-UTR) and a translated region. The 5'-UTR comprises the first intron of a gene that is native to an organism and expressed constitutively. Methods of using the expression constructs to enhance the expression of a gene in an organism and compositions comprising the expression constructs are further provided.
PCT/US2013/047837 2012-06-29 2013-06-26 Methods and compositions for enhancing gene expression WO2014004638A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201261666318P 2012-06-29 2012-06-29
US61/666,318 2012-06-29

Publications (2)

Publication Number Publication Date
WO2014004638A2 WO2014004638A2 (en) 2014-01-03
WO2014004638A3 true WO2014004638A3 (en) 2014-03-13

Family

ID=48746154

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2013/047837 WO2014004638A2 (en) 2012-06-29 2013-06-26 Methods and compositions for enhancing gene expression

Country Status (1)

Country Link
WO (1) WO2014004638A2 (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3259358B1 (en) * 2015-02-19 2024-06-19 Danisco US Inc. Enhanced protein expression
CA3050714A1 (en) 2017-01-19 2018-07-26 Monsanto Technology Llc Plant regulatory elements and uses thereof

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2000011185A2 (en) * 1998-08-19 2000-03-02 Monsanto Co. Improved expression of cry3b insecticidal protein in plants
EP2169058A2 (en) * 2005-03-08 2010-03-31 BASF Plant Science GmbH Expression enhancing intron sequences
WO2011156535A1 (en) * 2010-06-09 2011-12-15 E. I. Du Pont De Nemours And Company Regulatory sequences for modulating transgene expression in plants

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2000011185A2 (en) * 1998-08-19 2000-03-02 Monsanto Co. Improved expression of cry3b insecticidal protein in plants
EP1698699A2 (en) * 1998-08-19 2006-09-06 Monsanto Technology, LLC Improved expression of CRY3B insecticidal protein in plants
EP2169058A2 (en) * 2005-03-08 2010-03-31 BASF Plant Science GmbH Expression enhancing intron sequences
WO2011156535A1 (en) * 2010-06-09 2011-12-15 E. I. Du Pont De Nemours And Company Regulatory sequences for modulating transgene expression in plants

Also Published As

Publication number Publication date
WO2014004638A2 (en) 2014-01-03

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