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WO2014002956A1 - Container opening/closing device - Google Patents

Container opening/closing device Download PDF

Info

Publication number
WO2014002956A1
WO2014002956A1 PCT/JP2013/067263 JP2013067263W WO2014002956A1 WO 2014002956 A1 WO2014002956 A1 WO 2014002956A1 JP 2013067263 W JP2013067263 W JP 2013067263W WO 2014002956 A1 WO2014002956 A1 WO 2014002956A1
Authority
WO
WIPO (PCT)
Prior art keywords
container
lid member
containers
diluent
opening
Prior art date
Application number
PCT/JP2013/067263
Other languages
French (fr)
Japanese (ja)
Inventor
笹原　潤
陽介 村瀬
Original Assignee
協和メデックス株式会社
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 協和メデックス株式会社 filed Critical 協和メデックス株式会社
Priority to JP2014522621A priority Critical patent/JP6096187B2/en
Publication of WO2014002956A1 publication Critical patent/WO2014002956A1/en

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/02—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/02—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations
    • G01N35/04—Details of the conveyor system
    • G01N2035/0401—Sample carriers, cuvettes or reaction vessels
    • G01N2035/0403—Sample carriers with closing or sealing means
    • G01N2035/0405—Sample carriers with closing or sealing means manipulating closing or opening means, e.g. stoppers, screw caps, lids or covers
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/02—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations
    • G01N35/04—Details of the conveyor system
    • G01N2035/0439—Rotary sample carriers, i.e. carousels
    • G01N2035/0444—Rotary sample carriers, i.e. carousels for cuvettes or reaction vessels

Definitions

  • the present invention relates to a container opening / closing device that opens and closes the mouth of a container.
  • An immunoassay method for measuring a substance to be measured in a specimen using an antigen-antibody reaction is often used in clinical diagnosis.
  • a measurement target substance in a specimen in an immunoassay method for example, a primary antibody that reacts with the measurement target substance, a labeled secondary antibody that binds to a secondary antibody that reacts with the measurement target substance, and the specimen are combined.
  • an immune complex comprising a primary antibody, a substance to be measured, and a labeled secondary antibody is produced. Then, the measurement target substance in the sample is measured by measuring the amount of label in the generated immune complex.
  • the sample may need to be diluted with a diluent as a pretreatment.
  • a reagent a reagent such as a primary antibody-containing reagent and a labeled secondary antibody-containing reagent
  • a diluent contained in a container are aspirated by a dispensing nozzle. And discharged into the reaction vessel.
  • a dispensing nozzle In order to perform measurement accurately, it is necessary to prevent contamination of reagents, diluents, and specimens. Therefore, it is preferable to open the mouth of the container only when a dispensing nozzle is inserted.
  • Registered Utility Model No. 3029592 discloses a bottle opening / closing mechanism that opens and closes the mouths of two bottles one by one with a pivotable shutter.
  • the present invention has been made in view of such facts, and an object of the present invention is to provide a container opening / closing device capable of simultaneously opening and closing the mouths of a plurality of containers.
  • the invention of the first aspect comprises a plurality of container installation parts in which containers are arranged, a lid member that covers the mouth part of the container, and a drive means that moves the lid member to open and close the mouth part of the container,
  • the lid member is formed with a hole that allows insertion of the liquid suction member into the mouth portion of the container, one less than the number of the container installation portions, and the mouth portions of all the containers other than the outermost side and the When the holes are matched, the outermost mouth part of the container is not covered with the lid member, and when the hole is retracted from the mouth part of the container,
  • the container opening and closing device in which the hole is arranged so that the mouth of the container is covered with the lid member.
  • the mouths of all the containers other than the outermost side and the holes of the lid member are matched, the mouths of all the containers arranged in the container installation part are opened, and these Liquid suction members can be inserted into the mouths of the containers, and liquid suction from these containers becomes possible.
  • the lid member may be moved from a closed position where the mouth of the container is closed to an open position where the hole of the lid coincides with the mouth of the container. can do.
  • the mouths of all the containers other than the outermost and the holes of the lid member are matched, and the mouths of all the containers arranged in the container installation part are opened, the mouths of the outermost containers Since it is opened by not being covered with the lid member, the size of the lid member can be reduced.
  • the lid member may be moved from the open position where the hole of the lid member coincides with the mouth portion of the container to the closing position where the mouth portion of the container is closed. can do.
  • the container setting portion is arranged on a circumference, and the driving means turns and moves the lid member above the container setting portion.
  • the lid member is pivoted to open and close the mouth of the container, so that the lid member can be moved more efficiently than when the lid member is linearly moved to open and close the mouth of the container. Can do.
  • the driving means retreats the lid member from above the container by turning the lid member.
  • the container can be replaced smoothly by retracting the lid member from above the container.
  • a turning trajectory at the center of the hole is different from the circumference.
  • the mouths of a plurality of containers can be opened and closed simultaneously.
  • the immunoassay device 10 provided with the container opening / closing device 200 according to the embodiment of the present invention will be described with reference to the drawings.
  • the immunoassay device 10 includes a cell supply unit 12, a reagent storage unit 14, a reaction table 16, a sample table 18, a diluent storage unit 20, a BF unit 22, a measurement unit 24, a minute unit.
  • the apparatus includes a pouring device 26, 28, 30 and a transfer device 32, 34, 36.
  • the cell supply unit 12 is disposed at the left back of the immunoassay device 10.
  • the cell supply unit 12 includes a cell tank 38, an elevator 40, and a cell supply device 42 as a container supply device.
  • the cell supply device 42 includes an inclined conveyance path 44, a cell delivery device 46, and a cell standby place 48 as a container standby position.
  • the cells 50 are taken out one by one from the cell tank 38 stocked with a large number of empty reaction vessels (hereinafter referred to as “cells 50”) by the elevator 40, the inclined conveyance path 44, and the cell delivery device 46.
  • the cell 50 has a bottomed cylindrical portion made of resin with an open upper end, and two flanges that are provided at an intermediate position in the axial direction with respect to the upper end of the bottomed cylindrical portion and project over the entire circumference from the outer peripheral surface. is doing.
  • the reagent storage unit 14 is arranged on the left front side of the immunoassay device 10.
  • the reagent storage unit 14 is provided with a turntable 52, and a plurality of cassettes 54 are set on the turntable 52.
  • Each cassette 54 holds three reagent containers 56A, 56B, and 56C.
  • the reagent container 56A contains an insoluble carrier particle-containing reagent
  • the reagent container 56B contains a primary antibody-containing reagent
  • the reagent container 56C contains a labeled secondary antibody-containing reagent.
  • the reagent stored in the reagent containers 56A, 56B, 56C is cooled to a certain temperature by a cooling means (not shown).
  • the insoluble carrier particle-containing reagent contained in the reagent container 56A is stirred by a stirring device (not shown), and the insoluble carrier particles in the insoluble carrier particle-containing reagent are uniformly distributed. To maintain.
  • the reaction table 16 is arranged near the center of the immunoassay device 10.
  • the reaction table 16 is a disk-shaped member that can be rotated clockwise and counterclockwise, and a recess 58 that holds the cells 50 is provided at equal intervals in the circumferential direction in the entire outer peripheral portion of the reaction table 16. A plurality are formed.
  • the rotation of the reaction table 16 rotates and conveys the cell 50 held in the recess 58 to a predetermined position, and the cell 50 held in the recess 58 is heated by a heater (not shown). Accelerate the antigen-antibody reaction that occurs within 50.
  • the specimen table 18 is arranged on the front side of the reaction table 16.
  • a plurality of moving cassettes 60 that move automatically are set on the sample table 18.
  • the moving cassette 60 holds a plurality of test tubes 62 (10 in this embodiment) in which specimens are stored.
  • the diluent storage unit 20 is disposed between the reaction table 16 and the sample table 18.
  • the diluent storage unit 20 includes a container opening / closing device 200 and diluent containers 64A, 64B, and 64C as containers.
  • the BF unit 22 includes a BF table 66 and a BF cleaning device 68.
  • the BF table 66 is a disk-shaped member that can rotate clockwise and counterclockwise, and the concave portions 70 that hold the cells 50 are arranged at equal intervals in the circumferential direction in the entire outer peripheral portion of the BF table 66. A plurality are formed.
  • the cell 50 held in the recess 70 is rotated and conveyed to a predetermined position by the rotation of the BF table 66, and the inside of the cell 50 held in the recess 70 is B / F separated by the BF cleaning device 68. Wash with In this embodiment, an example in which two BF units 22 are arranged has been described, but any number of BF units 22 may be arranged.
  • the measurement unit 24 is arranged in front of the right side of the immunoassay device 10.
  • the measurement unit 24 includes a stirring unit 72 that stirs an immune complex and a detection reagent, which will be described later, housed in a cell 50, and a measurement chamber 74 that measures the amount of light.
  • the dispensing device 26 is disposed on the left side of the reaction table 16, the dispensing device 28 is disposed on the left front side of the reaction table 16, and the dispensing device 30 is disposed on the near side of the reaction table 16.
  • the dispensing devices 26, 28, and 30 are attached to the pivotable robot arms 76, 78, and 80, and the distal ends of the robot arms 76, 78, and 80, and automatically perform the liquid suction and discharge.
  • the transfer device 32 is disposed on the back side of the reaction table 16, the transfer device 34 is disposed on the right side of the reaction table 16, and the transfer device 36 is disposed on the left side of the measurement unit 24.
  • the transfer devices 32, 34, and 36 include pivotable robot arms 88, 90, and 92 and cell hands 94, 96, and 98 that are attached to the tips of the robot arms 88, 90, and 92 and hold the cell 50. Yes.
  • Cell supply unit 12 reagent storage unit 14, reaction table 16, sample table 18, diluent storage unit 20, BF unit 22, measurement unit 24, dispensing devices 26, 28, 30 and transfer devices 32, 34, 36 It operates automatically based on a control signal sent from a control unit (not shown).
  • a reagent containing streptavidin-bonded magnetic carrier particles is used as the reagent containing insoluble carrier particles.
  • the present invention is not limited to this, and a reagent containing other magnetic carrier particles may be used.
  • insoluble carrier particles having no magnetism, such as latex can be used.
  • a biotinylated primary antibody-containing reagent is used as the primary antibody-containing reagent.
  • the present invention is not limited thereto, and a reagent containing an antibody that is appropriately selected according to the type of the insoluble carrier particle-containing reagent is used. be able to.
  • the alkaline phosphatase-labeled secondary antibody-containing reagent is used as the labeled secondary antibody-containing reagent.
  • the present invention is not limited to this. Labeling with a labeling substance appropriately selected according to the type of the substance to be measured A reagent containing the prepared antibody can be used.
  • the chemiluminescence immunoassay based on the sandwich method is used as the immunoassay, but not limited to this, other immunoassays may be used.
  • the cells 50 are taken out one by one from the cell tank 38 in which a large number of empty cells 50 are stocked, and are placed in the cell waiting place 48.
  • the cell 50 arranged in the cell waiting place 48 is gripped by the cell hand 94 of the transfer device 32, conveyed by turning of the robot arm 88, and set in the recess 58 of the reaction table 16.
  • the cell 50 set in the recess 58 is rotated and conveyed by the reaction table 16 to the vicinity of the dispensing device 26 while being held in the recess 58.
  • the reagent containing the streptavidin-binding magnetic carrier particles is removed from the reagent container 56A held in the cassette 54 set on the turntable 52 by the dispensing nozzle 82 that is moved by the turning of the robot arm 76 of the dispensing device 26. Suction and discharge to the cell 50.
  • the biotinylated primary antibody-containing reagent is removed from the reagent container 56B held in the cassette 54 by the same method as that using the dispensing device 26. Suction is performed by a dispensing nozzle 82. Then, the biotinylated primary antibody-containing reagent sucked by the dispensing nozzle 82 is discharged to the cell 50 from which the streptavidin-bound magnetic carrier particle-containing reagent has been discharged.
  • the cell 50 from which the reagent containing streptavidin-bound magnetic carrier particles and the biotinylated primary antibody-containing reagent are discharged is rotated and conveyed to the vicinity of the dispensing device 30 by the reaction table 16 while being held in the recess 58. At this time, the inside of the cell 50 is heated to a predetermined temperature (for example, 37 ° C.) by a heater (not shown) provided on the reaction table 16, and the reaction between the streptavidin bound to the magnetic carrier particles and the biotinylated primary antibody. Is promoted.
  • a predetermined temperature for example, 37 ° C.
  • the sample is aspirated from the test tube 62 held in the moving cassette 60 set on the sample table 18 by the dispensing nozzle 86 that moves by the rotation of the robot arm 80 of the dispensing device 30. Then, the specimen sucked from the test tube 62 by the dispensing nozzle 86 is discharged to the cell 50 in which the streptavidin-binding magnetic carrier particle-containing reagent and the biotinylated primary antibody-containing reagent are discharged.
  • the diluent containers 64A, 64B, and 64C are opened, and the diluent is sucked from the diluent containers 64A, 64B, and 64C by the dispensing nozzle 86, and then The sample is aspirated from the test tube 62 set on the sample table 18. Then, a mixture of the diluted solution sucked from the diluent containers 64A, 64B, and 64C and the sample sucked from the test tube 62 is discharged to the cell 50. Thereafter, the dispensing nozzle 86 that discharges the mixture of the specimen and the diluted solution to the cell 50 is washed in the dispensing nozzle washing tank 102. Thereby, contamination by the sample in the dispensing nozzle 86 is prevented.
  • the sample 50 and the cell 50 in which the diluent is discharged as needed are rotated and conveyed to the stirring position 104 provided in the reaction table 16, and the reagent, the sample, and the sample in the cell 50 are used by the stirring device 106 as necessary.
  • the diluted solution is stirred without contact. Thereby, a complex composed of the primary antibody and the substance to be measured is formed on the magnetic carrier particles.
  • the alkaline phosphatase-labeled secondary antibody-containing reagent is removed from the reagent container 56C held in the cassette 54 set on the turntable 52 by the dispensing nozzle 84 that is moved by turning the robot arm 78 of the dispensing device 28. Suction. Then, the alkaline phosphatase-labeled secondary antibody-containing reagent sucked from the reagent container 56C by the dispensing nozzle 84 is transferred to the cell 50 containing the magnetic carrier particles on which the complex composed of the primary antibody and the substance to be measured is formed. Discharge. After dispensing the alkaline phosphatase-labeled secondary antibody-containing reagent, the dispensing nozzle 84 moves to the dispensing nozzle washing tank 108 and is washed.
  • the inside of the cell 50 from which the alkaline phosphatase-labeled secondary antibody-containing reagent has been discharged is stirred by the stirring device 106 to promote the reaction between the alkaline phosphatase-labeled secondary antibody and the complex composed of the primary antibody and the measurement target substance.
  • an immune complex composed of the primary antibody, the substance to be measured, and the alkaline phosphatase-labeled secondary antibody is formed on the magnetic carrier particles.
  • the cell 50 containing the magnetic carrier particles on which the immune complex is formed is rotated and conveyed to the vicinity of the transfer device 34 by the reaction table 16 while being held in the recess 58.
  • the cell 50 is held by the cell hand 96 of the transfer device 34, conveyed by turning the robot arm 90, and set in the recess 70 formed on the BF table 66 of the BF unit 22.
  • the reaction mixture in the cell 50 is separated into magnetic carrier particles on which the immune complex is formed and other substances (B / F separation).
  • the other substances are removed by a cleaning nozzle (not shown).
  • the magnetic carrier particles are washed by discharging a washing liquid into the cell 50 from a washing nozzle (not shown).
  • the washing liquid after washing the magnetic carrier particles is removed by a washing nozzle (not shown), whereby only the magnetic carrier particles on which the immune complex is formed remain in the cell 50.
  • the cell 50 that has been subjected to the B / F separation by the BF cleaning device 68 is gripped by the cell hand 98 of the transfer device 36, conveyed by turning of the robot arm 92, and set in the stirring unit 72 of the measurement unit 24.
  • a luminescent substrate reagent as a detection reagent is discharged into the cell 50 from a nozzle (not shown) provided in the cell hand 98.
  • the stirring unit 72 the mixed solution containing the immune complex and the luminescent substrate reagent in the set cell 50 is stirred, and alkaline phosphatase as a labeling substance in the immune complex reacts with the luminescent substrate reagent and emits light.
  • the cell 50 set in the stirring unit 72 is held by the cell hand 98 of the transfer device 36, conveyed by turning of the robot arm 92, and set in the measurement chamber 74 of the measurement unit 24.
  • the measurement chamber 74 the light emission amount generated by the reaction between the alkaline phosphatase in the immune complex and the luminescent substrate reagent is measured, and the concentration of the measurement target substance in the sample is determined from the measured light emission amount.
  • the cell 50 that has been measured is discarded to the disposal port 110 by the transfer device 36.
  • the diluent storage unit 20 includes a container opening / closing device 200 and diluent containers 64A and 64B as containers, as shown in the plan views of FIGS. 1 and 2 and the perspective view of FIG. , 64C.
  • the container opening / closing device 200 includes cylindrical container installation portions 202A, 202B, and 202C, a lid member 204, and a motor 206 as a driving unit.
  • the container installation portions 202 ⁇ / b> A, 202 ⁇ / b> B, and 202 ⁇ / b> C are provided on the upper surface of the ceiling portion 212 of the support base 210 installed on the base 208.
  • the three diluent containers 64A, 64B, 64C which each accommodated the different kind of diluent are set so that attachment or detachment is possible.
  • the diluent containers 64A, 64B, and 64C are on a circumference 216 having a radius R (the turning trajectory of the dispensing nozzle 86) centering on the turning center 214 of the robot arm 80 of the dispensing device 30.
  • R the turning trajectory of the dispensing nozzle 86
  • the specimen in the test tube 62 arranged on the specimen table 18 is aspirated, and the inside of the diluent containers 64A, 64B, and 64C is dispensed by the dispensing nozzle 86 of the dispensing device 30 that is washed in the dispensing nozzle washing tank 102.
  • Diluent containers 64A, 64B and 64C are arranged so that the diluted liquid can be sucked.
  • one dispensing operation is performed for a plurality of dispensing operations (the dispensing operation of the sample accommodated in the test tube 62 and the dispensing operation of the diluent contained in the diluent containers 64A, 64B, 64C).
  • the pouring device 30 can also be used.
  • any diluent can be used as long as it is used for diluting the specimen and enables accurate measurement of the measurement target substance in the specimen.
  • a protein an antiseptic, a surfactant, etc.
  • An aqueous medium containing Examples of proteins include bovine serum albumin and casein.
  • the preservative include sodium azide, antibiotics (streptomycin, penicillin, gentamicin, etc.), bioace, procrine 300, and the like.
  • the surfactant include a cationic surfactant, an anionic surfactant, an amphoteric surfactant, and a nonionic surfactant.
  • aqueous medium examples include deionized water, distilled water, and a buffer solution.
  • buffer used in the buffer examples include tris (hydroxymethyl) aminomethane buffer, phosphate buffer, borate buffer, Good's buffer, and the like.
  • Good buffering agents include, for example, 2-morpholinoethanesulfonic acid (MES), bis (2-hydroxyethyl) iminotris (hydroxymethyl) methane (Bis-Tris), N- (2-acetamido) iminodiacetic acid (ADA) Piperazine-N, N'-bis (2-ethanesulfonic acid) (PIPES), N- (2-acetamido) -2-aminoethanesulfonic acid (ACES), 3-morpholino-2-hydroxypropanesulfonic acid (MOPSO) ), N, N-bis (2-hydroxyethyl) -2-aminoethanesulfonic acid (BES), 3-morpholinopropanesulfonic acid (MOPS), N- [tris (hydroxymethyl) methyl] -2-aminoethanesulfone Acid (TES), 2- [4- (2-hydroxyethyl) -1-piperazinyl] ethanesulfonic acid (HEPE
  • a motor 206 as a driving unit is attached to the lower surface of the ceiling portion 212 of the support base 210 by projecting a rotating shaft 218 from the upper surface of the ceiling portion 212.
  • a disk-shaped light shielding member 220 is fixed to the rotating shaft 218 of the motor 206, and supports 222 and 224 are erected on the upper surface of the light shielding member 220. Further, as shown in FIG. 2, the upper portions of the columns 222 and 224 are formed by fan-shaped plates so as to cover the openings 226A, 226B, and 226C of the diluent containers 64A, 64B, and 64C as the mouth portions of the containers.
  • the lid member 204 is fixed.
  • the openings 226A, 226B, and 226C of the diluent containers 64A, 64B, and 64C are formed in a circular shape.
  • Rotating the motor 206 causes the light shielding member 220 to turn clockwise or counterclockwise around the rotation shaft 218 of the motor 206 as a rotation center. Accordingly, the lid member 204 integrated with the light shielding member 220 via the support columns 222 and 224 is interlocked with the light shielding member 220, and rotates clockwise or counterclockwise around the rotation shaft 218 of the motor 206. Turn to move. Then, the opening portions 226A, 226B, and 226C of the diluent containers 64A, 64B, and 64C are opened and closed by the movement of the lid member 204 by turning.
  • a rod-like stopper member 228 is fixed to the upper surface of the ceiling portion 212 of the support base 210 when the lid member 204 is turned in the counterclockwise direction.
  • a bar-shaped stopper member 230 is fixed to the upper surface of the ceiling portion 212 of the support base 210 to prevent the lid member 204 from pivoting in the clockwise direction.
  • the stopper portions 228 and 230 are omitted in FIG.
  • the lid member 204 has openings 226A and 226B that allow the dispensing nozzle 86 as a liquid suction member to be inserted into the openings 226A and 226B of the diluent containers 64A and 64B.
  • Two holes 232A and 232B having substantially the same size are formed. That is, the two holes 232A and 232B are formed by one less than the number of the three diluent containers 64A, 64B and 64C.
  • the two holes 232A and 232B are formed in the lid member 204 in this order from the reaction table 16 side to the sample table 18 side.
  • the holes 232A and 232B are arranged so that the hole 232B substantially coincides with the opening 226B of the diluent container 64B when the hole 232A is substantially coincident with the opening 226A of the diluent container 64A. Further, the holes 232A and 232B are arranged so that the turning trajectory at the center of the holes 232A and 232B due to the turning of the lid member 204 and the circumference 216 are shifted.
  • the motor 206 is driven to rotate and move the lid member 204 in the clockwise direction above the container setting portions 202A, 202B, 202C (diluent containers 64A, 64B, 64C).
  • the openings 226A and 226B of all the diluent containers 64A and 64B other than the diluent container 64C arranged on the outermost side on the specimen table 18 side and the holes 232A and 232B are substantially matched.
  • the lid member 204 is disposed so as not to cover the opening 226C of the diluent container 64C disposed on the outermost side on the specimen table 18 side. Thereby, the openings 226A, 226B, and 226C of all the diluent containers 64A, 64B, and 64C arranged in the container setting portions 202A, 202B, and 202C are opened.
  • the position of the lid member 204 at this time is defined as a container opening position.
  • the motor is moved from the state of FIG. 4A as shown in the plan view of FIG. 4B.
  • 206 is driven, and the lid member 204 is swung in the counterclockwise direction and moved above the container setting portions 202A, 202B, 202C (diluent containers 64A, 64B, 64C).
  • the holes 232A and 232B are retracted from the openings 226A and 226B of the diluent containers 64A and 64B.
  • the openings 226A, 226B, and 226C of all the diluent containers 64A, 64B, and 64C arranged in the container setting portions 202A, 202B, and 202C are covered with the lid member 204 to be closed.
  • the position of the lid member 204 at this time is set as a container closing position.
  • the motor 206 is driven from the state of FIG. 4A or FIG. 64A, 64B, 64C), the lid member 204 is pivoted and moved in the clockwise direction. By this movement, the lid member 204 is retracted from above the diluent containers 64A, 64B, and 64C arranged in the container setting sections 202A, 202B, and 202C. The position of the lid member 204 at this time is set as a container replacement position.
  • the position of the lid member 204 is detected by the two photoelectric sensors 234 and 236 installed on the upper surface of the ceiling portion 212 of the support base 210 and the light shielding member 220. .
  • the photoelectric sensors 234 and 236 are arranged at positions that are point-symmetric with respect to the rotation center 238 (rotation axis 218) of the light shielding member 220.
  • light shielding portions 240, 242, 244 and notches 246, 248, 250 are alternately arranged in the circumferential direction of the light shielding member 220.
  • the light shielding units 240 and 242 are disposed between the light projecting unit 252 and the light receiving unit 254 of the photoelectric sensors 234 and 236, the light emitted from the light projecting unit 252 is The light is blocked by the light blocking portions 240 and 242 and is not received by the light receiving portion 254.
  • this is referred to as “the light receiving portions 254 of the photoelectric sensors 234 and 236 are in a light shielding state”.
  • a notch 246 is arranged between the light projecting unit 252 and the light receiving unit 254 of the photoelectric sensor 234, A notch 250 is disposed between the light projecting unit 252 and the light receiving unit 254 of the photoelectric sensor 236. Then, the notch 246 is disposed between the light projecting unit 252 and the light receiving unit 254 of the photoelectric sensor 234, so that the light receiving unit 254 of the photoelectric sensor 234 is in a light incident state. Further, the notch portion 250 is disposed between the light projecting portion 252 and the light receiving portion 254 of the photoelectric sensor 236, so that the light receiving portion 254 of the photoelectric sensor 236 is in a light incident state.
  • the light shielding unit 242 is arranged between the light projecting unit 252 and the light receiving unit 254 of the photoelectric sensor 234, A light shielding unit 240 is disposed between the light projecting unit 252 and the light receiving unit 254 of the sensor 236. Then, the light shielding unit 242 is disposed between the light projecting unit 252 and the light receiving unit 254 of the photoelectric sensor 234, whereby the light receiving unit 254 of the photoelectric sensor 234 is in a light shielding state. In addition, since the light shielding unit 240 is disposed between the light projecting unit 252 and the light receiving unit 254 of the photoelectric sensor 236, the light receiving unit 254 of the photoelectric sensor 236 is in a light shielding state.
  • the light shielding unit 240 is arranged between the light projecting unit 252 and the light receiving unit 254 of the photoelectric sensor 234, A notch 248 is disposed between the light projecting unit 252 and the light receiving unit 254 of the sensor 236. Then, by arranging the light shielding unit 240 between the light projecting unit 252 and the light receiving unit 254 of the photoelectric sensor 234, the light receiving unit 254 of the photoelectric sensor 234 is in a light shielding state. Further, the notch portion 248 is disposed between the light projecting portion 252 and the light receiving portion 254 of the photoelectric sensor 236, so that the light receiving portion 254 of the photoelectric sensor 236 is in a light incident state.
  • the lid member 204 is disposed at the container open position. Further, when the light receiving unit 254 of the photoelectric sensor 234 is in a light shielding state and the light receiving unit 254 of the photoelectric sensor 236 is also in a light shielding state, it can be determined that the lid member 204 is disposed at the container closing position.
  • the lid member 204 is disposed at the container replacement position. In this way, the position of the lid member 204 is detected.
  • the arrangement of the cutout portion and the light shielding portion, and the combination of the light shielding portion and the light incident state of the light receiving portion with respect to the position of the lid member 204 are not limited to this example. It may be determined as appropriate.
  • the openings 226A and 226B of all the diluent containers 64A and 64B other than the diluent container 64C arranged on the outermost side on the specimen table 18 side are made to substantially coincide with the holes 232A and 232B.
  • the openings 226A, 226B, and 226C of all the diluent containers 64A, 64B, and 64C arranged in the container setting portions 202A, 202B, and 202C are opened.
  • a dispensing nozzle 86 as a liquid suction member can be inserted into these openings 226A, 226B, and 226C, and dilution liquid suction from these dilution liquid containers 64A, 64B, and 64C becomes possible.
  • the lid member 204 is moved from the container closing position (the position of the lid member 204 shown in FIG. 4B) where the openings 226A, 226B, and 226C of the diluent containers 64A, 64B, and 64C are closed.
  • the holes 232A and 232B of the lid member 204 may be moved to the container opening position (the position of the lid member 204 shown in FIG. 4A) that substantially coincides with the openings 226A and 226B of the diluent containers 64A and 64B. Therefore, the openings 226A, 226B, and 226C of the diluent containers 64A, 64B, and 64C can be opened with a small moving distance of the lid member 204. That is, the openings 226A, 226B, and 226C of the diluent containers 64A, 64B, and 64C can be opened in a short time.
  • a hole 232A is provided between the opening 226A of the adjacent diluent container 64A and the opening 226B of the diluent container 64B.
  • the opening of the diluent container 64A is about half the distance (the distance between the centers of the openings 226B of the diluent container 64B and the opening 226C of the diluent container 64C is about half. ),
  • the openings 226A, 226B, and 226C of the diluent containers 64A, 64B, and 64C can be opened by moving the lid member 204.
  • the openings 226A and 226B of all the diluent containers 64A and 64B other than the diluent container 64C arranged on the outermost side on the sample table 18 side are substantially matched with the holes 232A and 232B, so that the sample table 18 side
  • the openings of all the diluent containers 64A, 64B, and 64C arranged in the container installation portions 202A, 202B, and 202C The parts 226A, 226B, and 226C are opened. Thereby, the size of the lid member 204 can be reduced.
  • the holes 232A and 232B are shifted from the openings 226A and 226B of all the diluent containers 64A and 64B other than the diluent container 64C arranged on the outermost side on the specimen table 18 side.
  • the openings 226A, 226B, and 226C of all the diluent containers 64A, 64B, and 64C arranged in the installation sections 202A, 202B, and 202C With the lid member 204, the openings of these diluent containers 64A, 64B, and 64C are covered.
  • the parts 226A, 226B, 226C can be closed.
  • the lid member 204 has a container open position where the holes 232A and 232B of the lid member 204 substantially coincide with the openings 226A and 226B of the diluent containers 64A and 64B (the lid member 204 of the lid member 204 shown in FIG. 4A).
  • Position) to the container closing position where the openings 226A, 226B, and 226C of the diluent containers 64A, 64B, and 64C are closed (the position of the lid member 204 shown in FIG. 4B). Therefore, the openings 226A, 226B, and 226C of the diluent containers 64A, 64B, and 64C can be closed with a small moving distance of the lid member 204. That is, the openings 226A, 226B, and 226C of the diluent containers 64A, 64B, and 64C can be closed in a short time.
  • a hole 232A is provided between the opening 226A of the adjacent diluent container 64A and the opening 226B of the diluent container 64B.
  • the opening of the diluent container 64A The distance between the centers of 226A and the opening 226B of the diluent container 64B is about half the distance (the distance between the centers of the openings 226B of the diluent container 64B and the opening 226C of the diluent container 64C is about half. Only the lid member 204 needs to be moved.
  • the diluent members 64A, 64B, 64B, 64B, 64B, 64B, 64B, 64B, 64B, 64B, The replacement of 64C can be performed smoothly.
  • the number of holes 232A, 232B formed in the lid member 204 is one less than the number of diluent containers 64A, 64B, 64C. Therefore, the openings 226A, 226B, and 226C of all the diluent containers 64A, 64B, and 64C arranged in the container setting portions 202A, 202B, and 202C can be opened and closed with a small number of holes 232A and 232B.
  • the lid member 204 closes the openings 226A, 226B, and 226C of all the diluent containers 64A, 64B, and 64C that are set and arranged in the container setting portions 202A, 202B, and 202C. By doing so, it is possible to prevent the specimen from falling into the diluent container 64A, 64B, 64C from the dispensing nozzle 86 of the dispensing apparatus 30. Thereby, contamination of the diluent stored in the diluent containers 64A, 64B, 64C can be prevented.
  • the dilution liquid containers 64A, 64B, and 64C are arranged on the swirling trajectory of the dispensing nozzle 86, the risk of contamination of the dilution liquid increases. Therefore, application of the container opening / closing device 200 of the present embodiment is particularly effective.
  • opening and closing of the openings 226A, 226B, and 226C of all the diluent containers 64A, 64B, and 64C set and arranged in the container setting portions 202A, 202B, and 202C are performed on the lid member 204. This is done by turning movement. As a result, the lid member 204 is moved linearly, and the lid member 204 is efficiently moved with a simple mechanism as compared with the case where the openings 226A, 226B, and 226C of the diluent containers 64A, 64B, and 64C are opened and closed. Can be made.
  • the holes 232A and 232B are arranged so that the turning trajectory at the center of the holes 232A and 232B due to the turning of the lid member 204 and the circumference 216 are shifted.
  • the holes 232A and 232B are arranged at positions shifted inward or outward in the radial direction of the circumference 216. Therefore, the distance between the openings 226A, 226B, and 226C of the adjacent diluent containers 64A, 64B, and 64C on the circumference 216 can be made smaller than the diameter of the holes 232A and 232B. That is, the installation interval of the diluent containers 64A, 64B, 64C can be reduced.
  • diluent containers 64A, 64B, and 64C different types of diluents are stored in each of the diluent containers 64A, 64B, and 64C.
  • the same type of diluent is stored in each of the diluent containers 64A, 64B, and 64C.
  • a diluent may be accommodated.
  • diluent containers 64A, 64B, and 64C are arranged in the container setting sections 202A, 202B, and 202C.
  • two or more diluent containers may be arranged.
  • the number of holes formed in the lid member 204 is one less than the number of diluent containers.
  • the lid member 204 can have any shape as long as it can open and close the openings 226A, 226B, and 226C of all the diluent containers 64A, 64B, and 64C set and arranged in the container setting portions 202A, 202B, and 202C. There may be.
  • the diluent containers 64A, 64B, and 64C containing the diluent are used as containers.
  • the container of the present embodiment is also applied to containers containing other liquids.
  • the switchgear 200 can be applied.
  • the container opening / closing device is used in an immunoassay device 10 for immunoassay using three reagents (a reagent containing streptavidin-binding magnetic carrier particles, a reagent containing a biotinylated primary antibody, and a reagent containing an alkaline phosphatase-labeled antibody).
  • three reagents a reagent containing streptavidin-binding magnetic carrier particles, a reagent containing a biotinylated primary antibody, and a reagent containing an alkaline phosphatase-labeled antibody.
  • 200 is applied has been shown
  • the present invention is not limited to this.
  • This embodiment can be applied to an immunoassay apparatus for an immunoassay other than using the three reagents, or a measurement apparatus other than the immunoassay apparatus.
  • the present embodiment can be applied to devices for various uses that require opening and closing of the mouth of the container.

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Abstract

A container opening/closing device (200) provided with multiple container installation units (202A, 202B, 202C) on which containers are arranged, a lid member (204) for covering the openings of the containers, and a driving means (206) for opening and closing the openings of the containers by moving the lid member, wherein: holes (232A, 232B) for allowing a liquid absorption member (86) to be inserted into the openings of the container are formed on the lid member, the number of holes being less than the number of container installation units by one; and the holes are arranged such that the opening of the outermost container does not get covered by the lid member when all the openings of the containers except for the outermost container overlap with the holes, and such that the openings of all of the containers arranged on the container installation units are covered by the lid member when the holes are retreated from the openings of the containers.

Description

容器開閉装置Container opening and closing device
　本発明は、容器の口部を開閉する容器開閉装置に関する。 The present invention relates to a container opening / closing device that opens and closes the mouth of a container.
　抗原抗体反応を利用して検体中の測定対象物質を測定する免疫測定法が臨床診断においてしばしば用いられている。免疫測定法における検体中の測定対象物質の測定では、例えば、測定対象物質と反応する一次抗体と、測定対象物質と反応する二次抗体に標識が結合した標識化二次抗体と、検体とを反応容器中で反応させて、一次抗体、測定対象物質、及び標識化二次抗体からなる免疫複合体を生成させる。そして、生成した当該免疫複合体中の標識量を測定することにより、検体中の測定対象物質を測定する。免疫測定法において、測定対象成分によっては、前処理として、希釈液による検体の希釈が必要な場合がある。免疫測定法に使用される免疫測定装置では、容器に収容された、検体、試薬（一次抗体含有試薬、標識化二次抗体含有試薬等の試薬）、及び希釈液が、分注ノズルによって吸引されて反応容器に吐出される。測定を正確に行うためには、試薬、希釈液、及び検体のコンタミネーションを防ぐ必要があるので、分注ノズルを入れるときだけ容器の口部を開放状態にするのが好ましい。 An immunoassay method for measuring a substance to be measured in a specimen using an antigen-antibody reaction is often used in clinical diagnosis. In measurement of a measurement target substance in a specimen in an immunoassay method, for example, a primary antibody that reacts with the measurement target substance, a labeled secondary antibody that binds to a secondary antibody that reacts with the measurement target substance, and the specimen are combined. By reacting in a reaction container, an immune complex comprising a primary antibody, a substance to be measured, and a labeled secondary antibody is produced. Then, the measurement target substance in the sample is measured by measuring the amount of label in the generated immune complex. In the immunoassay, depending on the component to be measured, the sample may need to be diluted with a diluent as a pretreatment. In an immunoassay device used for an immunoassay, a specimen, a reagent (a reagent such as a primary antibody-containing reagent and a labeled secondary antibody-containing reagent), and a diluent contained in a container are aspirated by a dispensing nozzle. And discharged into the reaction vessel. In order to perform measurement accurately, it is necessary to prevent contamination of reagents, diluents, and specimens. Therefore, it is preferable to open the mouth of the container only when a dispensing nozzle is inserted.
　登録実用新案第３０２９５９２号公報には、旋回可能なシャッタによって２つのボトルの口部を一つ一つ開閉するボトル開閉機構が開示されている。 Registered Utility Model No. 3029592 discloses a bottle opening / closing mechanism that opens and closes the mouths of two bottles one by one with a pivotable shutter.
　しかし、登録実用新案第３０２９５９２号公報のボトル開閉機構では、２つのボトルの口部を同時に開閉するのが難しい。例えば、シャッタを２つのボトルの口部上から退避した位置に移動させて、２つのボトルの口部を同時に開閉しようとすると、開閉に時間が掛ってしまう。 However, with the bottle opening / closing mechanism of the registered utility model No. 3029592, it is difficult to simultaneously open and close the mouths of the two bottles. For example, if the shutter is moved to a position retracted from the mouths of the two bottles and the mouths of the two bottles are simultaneously opened and closed, it takes time to open and close.
　本発明は係る事実を考慮し、複数の容器の口部を同時に開閉することができる容器開閉装置を提供することを課題とする。 The present invention has been made in view of such facts, and an object of the present invention is to provide a container opening / closing device capable of simultaneously opening and closing the mouths of a plurality of containers.
　第１態様の発明は、容器が並べられる複数の容器設置部と、前記容器の口部を覆う蓋部材と、前記蓋部材を移動させ前記容器の口部を開閉させる駆動手段と、を備え、前記蓋部材には、前記容器の口部への液体吸引部材の挿入を許容する穴が前記容器設置部の数よりも１つ少なく形成され、最外側以外の全ての前記容器の口部と前記穴とを一致させたとき、最外側の前記容器の口部は前記蓋部材に覆われず、前記容器の口部から前記穴を退避させたとき、前記容器設置部に並べられた全ての前記容器の口部が前記蓋部材で覆われるように、前記穴が配置されている容器開閉装置である。 The invention of the first aspect comprises a plurality of container installation parts in which containers are arranged, a lid member that covers the mouth part of the container, and a drive means that moves the lid member to open and close the mouth part of the container, The lid member is formed with a hole that allows insertion of the liquid suction member into the mouth portion of the container, one less than the number of the container installation portions, and the mouth portions of all the containers other than the outermost side and the When the holes are matched, the outermost mouth part of the container is not covered with the lid member, and when the hole is retracted from the mouth part of the container, The container opening and closing device in which the hole is arranged so that the mouth of the container is covered with the lid member.
　第１態様の発明では、最外側以外の全ての容器の口部と蓋部材の穴とを一致させたときに、容器設置部に並べられた全ての容器の口部が開放されて、これらの容器の口部へ液体吸引部材を挿入することができ、これらの容器からの液吸引が可能になる。そして、このときに蓋部材は、容器の口部が閉止される閉止位置から、蓋部材の穴が容器の口部と一致する開放位置まで移動させればよいので、少ない移動距離で容器を開放することができる。 In the invention of the first aspect, when the mouths of all the containers other than the outermost side and the holes of the lid member are matched, the mouths of all the containers arranged in the container installation part are opened, and these Liquid suction members can be inserted into the mouths of the containers, and liquid suction from these containers becomes possible. At this time, the lid member may be moved from a closed position where the mouth of the container is closed to an open position where the hole of the lid coincides with the mouth of the container. can do.
　また、最外側以外の全ての容器の口部と蓋部材の穴とを一致させて、容器設置部に並べられた全ての容器の口部が開放されたときに、最外側の容器の口部は蓋部材に覆われないことによって開放されるので、蓋部材のサイズを小さくすることができる。 In addition, when the mouths of all the containers other than the outermost and the holes of the lid member are matched, and the mouths of all the containers arranged in the container installation part are opened, the mouths of the outermost containers Since it is opened by not being covered with the lid member, the size of the lid member can be reduced.
　さらに、最外側以外の全ての容器の口部から蓋部材の穴をずらし、容器設置部に並べられた全ての容器の口部を蓋部材で覆うことによって、容器設置部に並べられた全ての容器の口部を閉止することができる。そして、このときに蓋部材は、蓋部材の穴が容器の口部と一致する開放位置から、容器の口部が閉止される閉止位置まで移動させればよいので、少ない移動距離で容器を閉止することができる。 Furthermore, by shifting the holes of the lid members from the mouth portions of all containers other than the outermost side, covering all the mouth portions of the containers arranged in the container installation portion with the lid members, all the rows arranged in the container installation portion The mouth of the container can be closed. At this time, the lid member may be moved from the open position where the hole of the lid member coincides with the mouth portion of the container to the closing position where the mouth portion of the container is closed. can do.
　第２態様の発明は、第１態様の容器開閉装置において、前記容器設置部は、円周上に配置され、前記駆動手段は、前記容器設置部の上方で前記蓋部材を旋回させて移動させる。 According to a second aspect of the present invention, in the container opening and closing device according to the first aspect, the container setting portion is arranged on a circumference, and the driving means turns and moves the lid member above the container setting portion. .
　第２態様の発明では、蓋部材を旋回させて容器の口部を開閉させるので、蓋部材を直線移動させて容器の口部を開閉させる場合と比較して、効率よく蓋部材を移動させることができる。 In the second aspect of the invention, the lid member is pivoted to open and close the mouth of the container, so that the lid member can be moved more efficiently than when the lid member is linearly moved to open and close the mouth of the container. Can do.
　第３態様の発明は、第２態様の容器開閉装置において、前記駆動手段は、前記蓋部材を旋回させることにより、前記容器の上方から該蓋部材を退避させる。 According to a third aspect of the present invention, in the container opening / closing apparatus according to the second aspect, the driving means retreats the lid member from above the container by turning the lid member.
　第３態様の発明では、蓋部材を容器の上方から退避させることによって、容器の入れ替えをスムーズに行うことができる。 In the invention of the third aspect, the container can be replaced smoothly by retracting the lid member from above the container.
　第４態様の発明は、第２又は第３態様の容器開閉装置において、前記穴の中心の旋回軌道が、前記円周と異なっている。 According to the fourth aspect of the invention, in the container opening and closing device of the second or third aspect, a turning trajectory at the center of the hole is different from the circumference.
　第４態様の発明では、蓋部材を僅かに回転させるだけで、容易かつ確実に、容器設置部に並べられた全ての容器の口部を開放することも、閉止することもできる。 In the fourth aspect of the invention, it is possible to open and close the mouths of all the containers arranged in the container installation section easily and reliably by only slightly rotating the lid member.
　本発明は、上記の構成としたので、複数の容器の口部を同時に開閉することができる。 Since the present invention is configured as described above, the mouths of a plurality of containers can be opened and closed simultaneously.
実施形態に係る免疫測定装置を示す平面図である。It is a top view which shows the immunoassay apparatus which concerns on embodiment. 実施形態に係る希釈液保管ユニットを示す平面図である。It is a top view which shows the dilution liquid storage unit which concerns on embodiment. 実施形態に係る希釈液保管ユニットを示す斜視図である。It is a perspective view which shows the dilution liquid storage unit which concerns on embodiment. 実施形態に係る容器開閉装置の動作（容器開放位置に蓋部材が配置された状態）を示す平面図である。It is a top view which shows operation | movement (state with the cover member arrange | positioned in the container open position) of the container opening / closing apparatus which concerns on embodiment. 実施形態に係る容器開閉装置の動作（容器閉止位置に蓋部材が配置された状態）を示す平面図である。It is a top view which shows operation | movement (state with the cover member arrange | positioned in the container closing position) of the container opening / closing apparatus which concerns on embodiment. 実施形態に係る容器開閉装置の動作（容器交換位置に蓋部材が配置された状態）を示す平面図である。It is a top view which shows operation | movement (state with the cover member arrange | positioned in the container exchange position) of the container opening / closing apparatus which concerns on embodiment. 実施形態に係る蓋部材の位置検知方法（容器開放位置に蓋部材が配置された状態）を示す平面図である。It is a top view which shows the position detection method (state in which the cover member was arrange | positioned in the container open position) of the cover member which concerns on embodiment. 実施形態に係る蓋部材の位置検知方法（容器閉止位置に蓋部材が配置された状態）を示す平面図である。It is a top view which shows the position detection method (state in which the cover member was arrange | positioned in the container closing position) of the cover member which concerns on embodiment. 実施形態に係る蓋部材の位置検知方法（容器交換位置に蓋部材が配置された状態）を示す平面図である。It is a top view which shows the position detection method (state in which the cover member was arrange | positioned in the container exchange position) of the cover member which concerns on embodiment. 実施形態に係る光電センサ（投光部と受光部の間に遮光部が配置された状態）を示す斜視図である。It is a perspective view which shows the photoelectric sensor (state in which the light-shielding part is arrange | positioned between the light projection part and the light-receiving part) which concerns on embodiment. 実施形態に係る光電センサ（投光部と受光部の間に切り欠き部が配置された状態）を示す斜視図である。It is a perspective view which shows the photoelectric sensor (state in which the notch part was arrange | positioned between the light projection part and the light-receiving part) which concerns on embodiment. 実施形態に係る光電センサ（投光部と受光部の間に切り欠き部が配置された状態）を示す斜視図である。It is a perspective view which shows the photoelectric sensor (state in which the notch part was arrange | positioned between the light projection part and the light-receiving part) which concerns on embodiment.
（実施形態）
＜全体構成＞
　図を参照しながら、本発明の実施形態に係る容器開閉装置２００が備えられた免疫測定装置１０について説明する。図１の平面図に示すように、免疫測定装置１０には、セル供給ユニット１２、試薬保管ユニット１４、反応テーブル１６、検体テーブル１８、希釈液保管ユニット２０、ＢＦユニット２２、測定ユニット２４、分注装置２６、２８、３０、及び移送装置３２、３４、３６を有して構成されている。 (Embodiment)
<Overall configuration>
The immunoassay device 10 provided with the container opening / closing device 200 according to the embodiment of the present invention will be described with reference to the drawings. As shown in the plan view of FIG. 1, the immunoassay device 10 includes a cell supply unit 12, a reagent storage unit 14, a reaction table 16, a sample table 18, a diluent storage unit 20, a BF unit 22, a measurement unit 24, a minute unit. The apparatus includes a pouring device 26, 28, 30 and a transfer device 32, 34, 36.
　セル供給ユニット１２は、免疫測定装置１０の左奥に配置されている。セル供給ユニット１２は、セルタンク３８、エレベータ４０、及び容器供給装置としてのセル供給装置４２を有して構成されている。セル供給装置４２は、傾斜搬送路４４、セル送り出し装置４６、及び容器待機位置としてのセル待機所４８を有して構成されている。 The cell supply unit 12 is disposed at the left back of the immunoassay device 10. The cell supply unit 12 includes a cell tank 38, an elevator 40, and a cell supply device 42 as a container supply device. The cell supply device 42 includes an inclined conveyance path 44, a cell delivery device 46, and a cell standby place 48 as a container standby position.
　セル供給ユニット１２では、空の反応容器（以下、「セル５０」とする）が多数ストックされたセルタンク３８から、エレベータ４０、傾斜搬送路４４、及びセル送り出し装置４６によりセル５０を１つずつ取り出して、セル待機所４８へ配置する。セル５０は、上端が開口した樹脂製の有底円筒部と、有底円筒部の上端と軸方向中間の位置に設けられ、外周面から全周に渡って張り出す２つの鍔部とを有している。 In the cell supply unit 12, the cells 50 are taken out one by one from the cell tank 38 stocked with a large number of empty reaction vessels (hereinafter referred to as “cells 50”) by the elevator 40, the inclined conveyance path 44, and the cell delivery device 46. To the cell waiting place 48. The cell 50 has a bottomed cylindrical portion made of resin with an open upper end, and two flanges that are provided at an intermediate position in the axial direction with respect to the upper end of the bottomed cylindrical portion and project over the entire circumference from the outer peripheral surface. is doing.
　図１に示すように、試薬保管ユニット１４は、免疫測定装置１０の左手前に配置されている。試薬保管ユニット１４には、ターンテーブル５２が設けられており、このターンテーブル５２の上に複数のカセット５４がセットされている。各カセット５４には、３つの試薬容器５６Ａ、５６Ｂ、５６Ｃが保持されている。 As shown in FIG. 1, the reagent storage unit 14 is arranged on the left front side of the immunoassay device 10. The reagent storage unit 14 is provided with a turntable 52, and a plurality of cassettes 54 are set on the turntable 52. Each cassette 54 holds three reagent containers 56A, 56B, and 56C.
　試薬容器５６Ａには、不溶性担体粒子含有試薬が収容され、試薬容器５６Ｂには、一次抗体含有試薬が収容され、試薬容器５６Ｃには、標識化二次抗体含有試薬が収容されている。 The reagent container 56A contains an insoluble carrier particle-containing reagent, the reagent container 56B contains a primary antibody-containing reagent, and the reagent container 56C contains a labeled secondary antibody-containing reagent.
　試薬保管ユニット１４では、冷却手段（不図示）によって試薬容器５６Ａ、５６Ｂ、５６Ｃ内に収容される試薬を一定の温度に冷却する。また、試薬保管ユニット１４では、試薬容器５６Ａに収容されている不溶性担体粒子含有試薬を撹拌装置（不図示）によって撹拌し、不溶性担体粒子含有試薬中の不溶性担体粒子が均一に分布されている状態を維持する。 In the reagent storage unit 14, the reagent stored in the reagent containers 56A, 56B, 56C is cooled to a certain temperature by a cooling means (not shown). In the reagent storage unit 14, the insoluble carrier particle-containing reagent contained in the reagent container 56A is stirred by a stirring device (not shown), and the insoluble carrier particles in the insoluble carrier particle-containing reagent are uniformly distributed. To maintain.
　反応テーブル１６は、免疫測定装置１０の中央部付近に配置されている。反応テーブル１６は、時計回りと反時計回りとに回転可能な円板状の部材であり、反応テーブル１６の外周部全域には、セル５０を保持する凹部５８が周方向に対して等間隔に複数形成されている。 The reaction table 16 is arranged near the center of the immunoassay device 10. The reaction table 16 is a disk-shaped member that can be rotated clockwise and counterclockwise, and a recess 58 that holds the cells 50 is provided at equal intervals in the circumferential direction in the entire outer peripheral portion of the reaction table 16. A plurality are formed.
　反応テーブル１６では、この反応テーブル１６の回転により、凹部５８に保持されたセル５０を所定の位置に回転搬送するとともに、凹部５８に保持されたセル５０をヒーター（不図示）により温めて、セル５０内で生じさせる抗原抗体反応を促進する。 In the reaction table 16, the rotation of the reaction table 16 rotates and conveys the cell 50 held in the recess 58 to a predetermined position, and the cell 50 held in the recess 58 is heated by a heater (not shown). Accelerate the antigen-antibody reaction that occurs within 50.
　検体テーブル１８は、反応テーブル１６の手前側に配置されている。検体テーブル１８の上には、自動的に移動する複数の移動カセット６０がセットされている。また、移動カセット６０には、検体が収容された試験管６２が複数（本実施形態では、１０本）保持されている。 The specimen table 18 is arranged on the front side of the reaction table 16. A plurality of moving cassettes 60 that move automatically are set on the sample table 18. In addition, the moving cassette 60 holds a plurality of test tubes 62 (10 in this embodiment) in which specimens are stored.
　希釈液保管ユニット２０は、反応テーブル１６と検体テーブル１８との間に配置されている。希釈液保管ユニット２０は、容器開閉装置２００と、容器としての希釈液容器６４Ａ、６４Ｂ、６４Ｃとを有して構成されている。 The diluent storage unit 20 is disposed between the reaction table 16 and the sample table 18. The diluent storage unit 20 includes a container opening / closing device 200 and diluent containers 64A, 64B, and 64C as containers.
　ＢＦユニット２２は、反応テーブル１６の右側に２つ配置されている。ＢＦユニット２２は、ＢＦテーブル６６と、ＢＦ洗浄装置６８とを有して構成されている。ＢＦテーブル６６は、時計回りと反時計回りとに回転可能な円板状の部材であり、ＢＦテーブル６６の外周部全域には、セル５０を保持する凹部７０が周方向に対して等間隔に複数形成されている。 Two BF units 22 are arranged on the right side of the reaction table 16. The BF unit 22 includes a BF table 66 and a BF cleaning device 68. The BF table 66 is a disk-shaped member that can rotate clockwise and counterclockwise, and the concave portions 70 that hold the cells 50 are arranged at equal intervals in the circumferential direction in the entire outer peripheral portion of the BF table 66. A plurality are formed.
　ＢＦユニット２２では、ＢＦテーブル６６の回転により、凹部７０に保持されたセル５０を所定の位置に回転搬送するとともに、ＢＦ洗浄装置６８によって、凹部７０に保持されたセル５０内をＢ／Ｆ分離により洗浄する。なお、本実施形態では、２つのＢＦユニット２２が配置されている例を示したが、配置するＢＦユニット２２は幾つでもよい。 In the BF unit 22, the cell 50 held in the recess 70 is rotated and conveyed to a predetermined position by the rotation of the BF table 66, and the inside of the cell 50 held in the recess 70 is B / F separated by the BF cleaning device 68. Wash with In this embodiment, an example in which two BF units 22 are arranged has been described, but any number of BF units 22 may be arranged.
　測定ユニット２４は、免疫測定装置１０の右手前に配置されている。測定ユニット２４は、セル５０内に収容された、後に説明する免疫複合体と検出試薬とを撹拌する撹拌部７２と、光量を測定する測定室７４とを有して構成されている。 The measurement unit 24 is arranged in front of the right side of the immunoassay device 10. The measurement unit 24 includes a stirring unit 72 that stirs an immune complex and a detection reagent, which will be described later, housed in a cell 50, and a measurement chamber 74 that measures the amount of light.
　分注装置２６は、反応テーブル１６の左側に配置され、分注装置２８は、反応テーブル１６の左手前側に配置され、分注装置３０は、反応テーブル１６の手前側に配置されている。 The dispensing device 26 is disposed on the left side of the reaction table 16, the dispensing device 28 is disposed on the left front side of the reaction table 16, and the dispensing device 30 is disposed on the near side of the reaction table 16.
　分注装置２６、２８、３０は、旋回可能なロボットアーム７６、７８、８０と、このロボットアーム７６、７８、８０の先端に取り付けられ、液体の吸引と吐出しとを自動的に行う分注ノズル８２、８４、８６とを備えている。 The dispensing devices 26, 28, and 30 are attached to the pivotable robot arms 76, 78, and 80, and the distal ends of the robot arms 76, 78, and 80, and automatically perform the liquid suction and discharge. Nozzles 82, 84, 86.
　移送装置３２は、反応テーブル１６の奥側に配置され、移送装置３４は、反応テーブル１６の右側に配置され、移送装置３６は、測定ユニット２４の左側に配置されている。 The transfer device 32 is disposed on the back side of the reaction table 16, the transfer device 34 is disposed on the right side of the reaction table 16, and the transfer device 36 is disposed on the left side of the measurement unit 24.
　移送装置３２、３４、３６は、旋回可能なロボットアーム８８、９０、９２と、このロボットアーム８８、９０、９２の先端に取り付けられ、セル５０を把持するセルハンド９４、９６、９８とを備えている。 The transfer devices 32, 34, and 36 include pivotable robot arms 88, 90, and 92 and cell hands 94, 96, and 98 that are attached to the tips of the robot arms 88, 90, and 92 and hold the cell 50. Yes.
　セル供給ユニット１２、試薬保管ユニット１４、反応テーブル１６、検体テーブル１８、希釈液保管ユニット２０、ＢＦユニット２２、測定ユニット２４、分注装置２６、２８、３０、及び移送装置３２、３４、３６は、制御部（不図示）から送られる制御信号に基づいて自動的に動作する。 Cell supply unit 12, reagent storage unit 14, reaction table 16, sample table 18, diluent storage unit 20, BF unit 22, measurement unit 24, dispensing devices 26, 28, 30 and transfer devices 32, 34, 36 It operates automatically based on a control signal sent from a control unit (not shown).
＜免疫測定法＞
　免疫測定装置１０を用いた免疫測定法の一例を説明する。なお、本実施態様では、不溶性担体粒子含有試薬としてストレプトアビジン結合磁性担体粒子含有試薬を用いているが、これに限らず、他の磁性担体粒子を含有する試薬を用いてもよい。また、磁石を用いずにＢ／Ｆ分離を行う場合は、磁性を有しない不溶性担体粒子、例えば、ラテックス等を用いることができる。さらに、本実施態様では、一次抗体含有試薬としてビオチン化一次抗体含有試薬を用いているが、これに限らず、不溶性担体粒子含有試薬の種類に応じて適宜選択される抗体を含有する試薬を用いることができる。また、本実施態様では、標識化二次抗体含有試薬としてアルカリホスファターゼ標識二次抗体含有試薬を用いているが、これに限らず、測定対象物質の種類に応じて適宜選択される標識物質で標識された抗体を含有する試薬を用いることができる。さらに、本実施態様においては、免疫測定法として、サンドイッチ法に基づく化学発光免疫測定法を用いているが、これに限らず、他の免疫測定法を用いてもよい。 <Immunoassay>
An example of an immunoassay method using the immunoassay device 10 will be described. In this embodiment, a reagent containing streptavidin-bonded magnetic carrier particles is used as the reagent containing insoluble carrier particles. However, the present invention is not limited to this, and a reagent containing other magnetic carrier particles may be used. Moreover, when performing B / F separation without using a magnet, insoluble carrier particles having no magnetism, such as latex, can be used. Furthermore, in this embodiment, a biotinylated primary antibody-containing reagent is used as the primary antibody-containing reagent. However, the present invention is not limited thereto, and a reagent containing an antibody that is appropriately selected according to the type of the insoluble carrier particle-containing reagent is used. be able to. In this embodiment, the alkaline phosphatase-labeled secondary antibody-containing reagent is used as the labeled secondary antibody-containing reagent. However, the present invention is not limited to this. Labeling with a labeling substance appropriately selected according to the type of the substance to be measured A reagent containing the prepared antibody can be used. Furthermore, in this embodiment, the chemiluminescence immunoassay based on the sandwich method is used as the immunoassay, but not limited to this, other immunoassays may be used.
　初めに、セル供給ユニット１２において、空のセル５０が多数ストックされたセルタンク３８からセル５０が１つずつ取り出されて、セル待機所４８に配置される。 First, in the cell supply unit 12, the cells 50 are taken out one by one from the cell tank 38 in which a large number of empty cells 50 are stocked, and are placed in the cell waiting place 48.
　次に、セル待機所４８に配置されたセル５０は、移送装置３２のセルハンド９４に把持され、ロボットアーム８８の旋回により搬送されて、反応テーブル１６の凹部５８へセットされる。そして、凹部５８へセットされたセル５０は、凹部５８に保持された状態で、反応テーブル１６により分注装置２６の近傍へ回転搬送される。 Next, the cell 50 arranged in the cell waiting place 48 is gripped by the cell hand 94 of the transfer device 32, conveyed by turning of the robot arm 88, and set in the recess 58 of the reaction table 16. The cell 50 set in the recess 58 is rotated and conveyed by the reaction table 16 to the vicinity of the dispensing device 26 while being held in the recess 58.
　次に、分注装置２６のロボットアーム７６の旋回によって移動する分注ノズル８２により、ターンテーブル５２の上にセットされたカセット５４に保持された試薬容器５６Ａからストレプトアビジン結合磁性担体粒子含有試薬を吸引して、このセル５０へ吐出する。 Next, the reagent containing the streptavidin-binding magnetic carrier particles is removed from the reagent container 56A held in the cassette 54 set on the turntable 52 by the dispensing nozzle 82 that is moved by the turning of the robot arm 76 of the dispensing device 26. Suction and discharge to the cell 50.
　次に、分注ノズル８２を分注ノズル洗浄槽１００で洗浄した後に、分注装置２６を用いた先と同様の方法によって、カセット５４に保持された試薬容器５６Ｂからビオチン化一次抗体含有試薬を分注ノズル８２により吸引する。そして、分注ノズル８２により吸引したビオチン化一次抗体含有試薬を、ストレプトアビジン結合磁性担体粒子含有試薬を吐出したセル５０へ吐出する。 Next, after the dispensing nozzle 82 is washed in the dispensing nozzle washing tank 100, the biotinylated primary antibody-containing reagent is removed from the reagent container 56B held in the cassette 54 by the same method as that using the dispensing device 26. Suction is performed by a dispensing nozzle 82. Then, the biotinylated primary antibody-containing reagent sucked by the dispensing nozzle 82 is discharged to the cell 50 from which the streptavidin-bound magnetic carrier particle-containing reagent has been discharged.
　ストレプトアビジン結合磁性担体粒子含有試薬とビオチン化一次抗体含有試薬とが吐出されたセル５０は、凹部５８に保持された状態で、反応テーブル１６により分注装置３０の近傍へ回転搬送される。このとき、セル５０内は反応テーブル１６に設けられたヒータ（不図示）により所定の温度（例えば、３７℃）に温められ、磁性担体粒子に結合されたストレプトアビジンとビオチン化一次抗体との反応が促進される。 The cell 50 from which the reagent containing streptavidin-bound magnetic carrier particles and the biotinylated primary antibody-containing reagent are discharged is rotated and conveyed to the vicinity of the dispensing device 30 by the reaction table 16 while being held in the recess 58. At this time, the inside of the cell 50 is heated to a predetermined temperature (for example, 37 ° C.) by a heater (not shown) provided on the reaction table 16, and the reaction between the streptavidin bound to the magnetic carrier particles and the biotinylated primary antibody. Is promoted.
　次に、分注装置３０のロボットアーム８０の旋回によって移動する分注ノズル８６により、検体テーブル１８の上にセットされた移動カセット６０に保持された試験管６２から検体を吸引する。そして、分注ノズル８６により試験管６２から吸引した検体を、ストレプトアビジン結合磁性担体粒子含有試薬とビオチン化一次抗体含有試薬とが吐出されたセル５０へ吐出する。また、測定において希釈液を用いる場合には、希釈液容器６４Ａ、６４Ｂ、６４Ｃを開放状態とした後、分注ノズル８６により、希釈液容器６４Ａ、６４Ｂ、６４Ｃから希釈液を吸引し、次いで、検体テーブル１８にセットされた試験管６２から検体を吸引する。そして、希釈液容器６４Ａ、６４Ｂ、６４Ｃから吸引した希釈液と、試験管６２から吸引した検体との混合物をセル５０へ吐出する。その後、検体と希釈液の混合物をセル５０へ吐出した分注ノズル８６は、分注ノズル洗浄槽１０２で洗浄される。これにより、分注ノズル８６における検体による汚染が防止される。 Next, the sample is aspirated from the test tube 62 held in the moving cassette 60 set on the sample table 18 by the dispensing nozzle 86 that moves by the rotation of the robot arm 80 of the dispensing device 30. Then, the specimen sucked from the test tube 62 by the dispensing nozzle 86 is discharged to the cell 50 in which the streptavidin-binding magnetic carrier particle-containing reagent and the biotinylated primary antibody-containing reagent are discharged. In the case of using a diluent for measurement, the diluent containers 64A, 64B, and 64C are opened, and the diluent is sucked from the diluent containers 64A, 64B, and 64C by the dispensing nozzle 86, and then The sample is aspirated from the test tube 62 set on the sample table 18. Then, a mixture of the diluted solution sucked from the diluent containers 64A, 64B, and 64C and the sample sucked from the test tube 62 is discharged to the cell 50. Thereafter, the dispensing nozzle 86 that discharges the mixture of the specimen and the diluted solution to the cell 50 is washed in the dispensing nozzle washing tank 102. Thereby, contamination by the sample in the dispensing nozzle 86 is prevented.
　検体、及び、必要に応じて希釈液が吐出されたセル５０は、反応テーブル１６に設けられた撹拌位置１０４まで回転搬送され、撹拌装置１０６によりセル５０内の試薬、検体、及び、必要に応じて希釈液が非接触で撹拌される。これにより、磁性担体粒子上に、一次抗体及び測定対象物質からなる複合体が形成される。 The sample 50 and the cell 50 in which the diluent is discharged as needed are rotated and conveyed to the stirring position 104 provided in the reaction table 16, and the reagent, the sample, and the sample in the cell 50 are used by the stirring device 106 as necessary. The diluted solution is stirred without contact. Thereby, a complex composed of the primary antibody and the substance to be measured is formed on the magnetic carrier particles.
　次に、分注装置２８のロボットアーム７８の旋回によって移動する分注ノズル８４により、ターンテーブル５２の上にセットされたカセット５４に保持された試薬容器５６Ｃからアルカリホスファターゼ標識二次抗体含有試薬を吸引する。そして、分注ノズル８４により試薬容器５６Ｃから吸引されたアルカリホスファターゼ標識二次抗体含有試薬を、一次抗体及び測定対象物質からなる複合体がその上に形成された磁性担体粒子を含有するセル５０へ吐出する。分注ノズル８４は、アルカリホスファターゼ標識二次抗体含有試薬を吐出した後、分注ノズル洗浄槽１０８に移動して洗浄される。 Next, the alkaline phosphatase-labeled secondary antibody-containing reagent is removed from the reagent container 56C held in the cassette 54 set on the turntable 52 by the dispensing nozzle 84 that is moved by turning the robot arm 78 of the dispensing device 28. Suction. Then, the alkaline phosphatase-labeled secondary antibody-containing reagent sucked from the reagent container 56C by the dispensing nozzle 84 is transferred to the cell 50 containing the magnetic carrier particles on which the complex composed of the primary antibody and the substance to be measured is formed. Discharge. After dispensing the alkaline phosphatase-labeled secondary antibody-containing reagent, the dispensing nozzle 84 moves to the dispensing nozzle washing tank 108 and is washed.
　アルカリホスファターゼ標識二次抗体含有試薬が吐出されたセル５０内は、撹拌装置１０６により撹拌され、アルカリホスファターゼ標識二次抗体と、一次抗体及び測定対象物質からなる複合体との反応が促進される。これにより、磁性担体粒子上に、一次抗体、測定対象物質及びアルカリホスファターゼ標識二次抗体からなる免疫複合体が形成される。 The inside of the cell 50 from which the alkaline phosphatase-labeled secondary antibody-containing reagent has been discharged is stirred by the stirring device 106 to promote the reaction between the alkaline phosphatase-labeled secondary antibody and the complex composed of the primary antibody and the measurement target substance. As a result, an immune complex composed of the primary antibody, the substance to be measured, and the alkaline phosphatase-labeled secondary antibody is formed on the magnetic carrier particles.
　次に、免疫複合体がその上に形成された磁性担体粒子を含有するセル５０は、凹部５８に保持された状態で、反応テーブル１６により移送装置３４の近傍へ回転搬送される。そして、このセル５０は、移送装置３４のセルハンド９６に把持され、ロボットアーム９０の旋回により搬送されて、ＢＦユニット２２のＢＦテーブル６６に形成された凹部７０へセットされる。 Next, the cell 50 containing the magnetic carrier particles on which the immune complex is formed is rotated and conveyed to the vicinity of the transfer device 34 by the reaction table 16 while being held in the recess 58. The cell 50 is held by the cell hand 96 of the transfer device 34, conveyed by turning the robot arm 90, and set in the recess 70 formed on the BF table 66 of the BF unit 22.
　次に、ＢＦ洗浄装置６８において、磁石を用いて、このセル５０内の反応混合物を、免疫複合体がその上に形成された磁性担体粒子とそれ以外の物質とに分離（Ｂ／Ｆ分離）し、当該それ以外の物質を洗浄ノズル（不図示）で除去する。さらに、セル５０内に洗浄ノズル（不図示）から洗浄液を吐出して磁性担体粒子を洗浄する。磁性担体粒子を洗浄した後の洗浄液は洗浄ノズル（不図示）で除去され、これにより、このセル５０内には、免疫複合体がその上に形成された磁性担体粒子のみが残留する。 Next, in the BF cleaning device 68, using a magnet, the reaction mixture in the cell 50 is separated into magnetic carrier particles on which the immune complex is formed and other substances (B / F separation). The other substances are removed by a cleaning nozzle (not shown). Further, the magnetic carrier particles are washed by discharging a washing liquid into the cell 50 from a washing nozzle (not shown). The washing liquid after washing the magnetic carrier particles is removed by a washing nozzle (not shown), whereby only the magnetic carrier particles on which the immune complex is formed remain in the cell 50.
　次に、ＢＦ洗浄装置６８によりＢ／Ｆ分離が行われたセル５０は、移送装置３６のセルハンド９８に把持され、ロボットアーム９２の旋回により搬送されて、測定ユニット２４の撹拌部７２にセットされる。このとき、セル５０には、セルハンド９８に設けられたノズル（不図示）から検出試薬としての発光基質試薬が吐出される。撹拌部７２では、セットされたセル５０内の免疫複合体と発光基質試薬とを含む混合液が撹拌され、免疫複合体中の標識物質としてのアルカリホスファターゼが発光基質試薬と反応して発光する。 Next, the cell 50 that has been subjected to the B / F separation by the BF cleaning device 68 is gripped by the cell hand 98 of the transfer device 36, conveyed by turning of the robot arm 92, and set in the stirring unit 72 of the measurement unit 24. The At this time, a luminescent substrate reagent as a detection reagent is discharged into the cell 50 from a nozzle (not shown) provided in the cell hand 98. In the stirring unit 72, the mixed solution containing the immune complex and the luminescent substrate reagent in the set cell 50 is stirred, and alkaline phosphatase as a labeling substance in the immune complex reacts with the luminescent substrate reagent and emits light.
　次に、撹拌部７２にセットされたセル５０は、移送装置３６のセルハンド９８に把持され、ロボットアーム９２の旋回により搬送されて、測定ユニット２４の測定室７４内へセットされる。測定室７４では、免疫複合体中のアルカリホスファターゼと発光基質試薬との反応により生成した光の発光量が測定され、この測定された発光量から検体中の測定対象物質の濃度を決定する。測定が終わったセル５０は、移送装置３６により廃棄口１１０へ廃棄される。 Next, the cell 50 set in the stirring unit 72 is held by the cell hand 98 of the transfer device 36, conveyed by turning of the robot arm 92, and set in the measurement chamber 74 of the measurement unit 24. In the measurement chamber 74, the light emission amount generated by the reaction between the alkaline phosphatase in the immune complex and the luminescent substrate reagent is measured, and the concentration of the measurement target substance in the sample is determined from the measured light emission amount. The cell 50 that has been measured is discarded to the disposal port 110 by the transfer device 36.
＜希釈液保管ユニットの構成＞
　全体構成で説明したように、希釈液保管ユニット２０は、図１、図２の平面図、及び図３の斜視図に示すように、容器開閉装置２００と、容器としての希釈液容器６４Ａ、６４Ｂ、６４Ｃとを有して構成されている。また、容器開閉装置２００は、円筒状の容器設置部２０２Ａ、２０２Ｂ、２０２Ｃと、蓋部材２０４と、駆動手段としてのモータ２０６とを有して構成されている。 <Configuration of dilution liquid storage unit>
As described in the overall configuration, the diluent storage unit 20 includes a container opening / closing device 200 and diluent containers 64A and 64B as containers, as shown in the plan views of FIGS. 1 and 2 and the perspective view of FIG. , 64C. The container opening / closing device 200 includes cylindrical container installation portions 202A, 202B, and 202C, a lid member 204, and a motor 206 as a driving unit.
　図２及び図３に示すように、容器設置部２０２Ａ、２０２Ｂ、２０２Ｃは、基台２０８の上に設置された支持台２１０の天井部２１２の上面に設けられている。そして、この容器設置部２０２Ａ、２０２Ｂ、２０２Ｃ内に、異なった種類の希釈液がそれぞれ収容されている３つの希釈液容器６４Ａ、６４Ｂ、６４Ｃが着脱可能にセットされている。 As shown in FIGS. 2 and 3, the container installation portions 202 </ b> A, 202 </ b> B, and 202 </ b> C are provided on the upper surface of the ceiling portion 212 of the support base 210 installed on the base 208. And in this container installation part 202A, 202B, 202C, the three diluent containers 64A, 64B, 64C which each accommodated the different kind of diluent are set so that attachment or detachment is possible.
　図２に示すように、希釈液容器６４Ａ、６４Ｂ、６４Ｃは、分注装置３０のロボットアーム８０の旋回中心２１４を中心とする半径Ｒの円周２１６（分注ノズル８６の旋回軌道）上に配置されている。希釈液容器６４Ａ、６４Ｂ、６４Ｃは、反応テーブル１６側から検体テーブル１８側へ向かってこの順に等間隔に配置されている。すなわち、検体テーブル１８に配置された試験管６２内の検体の吸引を行い、分注ノズル洗浄槽１０２で洗浄される分注装置３０の分注ノズル８６によって、希釈液容器６４Ａ、６４Ｂ、６４Ｃ内の希釈液の吸引を行うことができるように、希釈液容器６４Ａ、６４Ｂ、６４Ｃが配置されている。これによって、複数の分注作業（試験管６２に収容されている検体の分注作業と、希釈液容器６４Ａ、６４Ｂ、６４Ｃに収容されている希釈液の分注作業）に対して１つの分注装置３０を兼用することができる。 As shown in FIG. 2, the diluent containers 64A, 64B, and 64C are on a circumference 216 having a radius R (the turning trajectory of the dispensing nozzle 86) centering on the turning center 214 of the robot arm 80 of the dispensing device 30. Has been placed. The diluent containers 64A, 64B, and 64C are arranged at equal intervals in this order from the reaction table 16 side to the sample table 18 side. That is, the specimen in the test tube 62 arranged on the specimen table 18 is aspirated, and the inside of the diluent containers 64A, 64B, and 64C is dispensed by the dispensing nozzle 86 of the dispensing device 30 that is washed in the dispensing nozzle washing tank 102. Diluent containers 64A, 64B and 64C are arranged so that the diluted liquid can be sucked. Thus, one dispensing operation is performed for a plurality of dispensing operations (the dispensing operation of the sample accommodated in the test tube 62 and the dispensing operation of the diluent contained in the diluent containers 64A, 64B, 64C). The pouring device 30 can also be used.
　希釈液は、検体の希釈に使用され、検体中の測定対象物質の正確な測定を可能とする希釈液であれば、いかなる希釈液を用いることができ、例えば蛋白質、防腐剤、界面活性剤等を含む水性媒体等が挙げられる。蛋白質としては、例えば牛血清アルブミン、カゼイン等が挙げられる。防腐剤としては、例えばアジ化ナトリウム、抗生物質（ストレプトマイシン、ペニシリン、ゲンタマイシン等）、バイオエース、プロクリン300等が挙げられる。界面活性剤としては、例えば陽イオン性界面活性剤、陰イオン性界面活性剤、両性界面活性剤、非イオン性界面活性剤等が挙げられる。水性媒体としては、例えば脱イオン水、蒸留水、緩衝液等が挙げられる。緩衝液に用いる緩衝剤としては、例えばトリス（ヒドロキシメチル）アミノメタン緩衝剤、リン酸緩衝剤、ホウ酸緩衝剤、グッドの緩衝剤等が挙げられる。グッドの緩衝剤としては、例えば2-モルホリノエタンスルホン酸(MES)、ビス（2-ヒドロキシエチル）イミノトリス（ヒドロキシメチル）メタン(Bis-Tris)、N-（2-アセトアミド）イミノ二酢酸(ADA)、ピペラジン-N,N’-ビス（2-エタンスルホン酸）(PIPES)、N-（2-アセトアミド）-2-アミノエタンスルホン酸(ACES)、3-モルホリノ-2-ヒドロキシプロパンスルホン酸(MOPSO)、N,N-ビス（2-ヒドロキシエチル）-2-アミノエタンスルホン酸(BES)、3-モルホリノプロパンスルホン酸(MOPS)、N-〔トリス（ヒドロキシメチル）メチル〕-2-アミノエタンスルホン酸(TES)、2-〔4-（2-ヒドロキシエチル）-1-ピペラジニル〕エタンスルホン酸(HEPES)、3-〔N,N-ビス（2-ヒドロキシエチル）アミノ〕-2-ヒドロキシプロパンスルホン酸(DIPSO)、N-〔トリス（ヒドロキシメチル）メチル〕-2-ヒドロキシ-3-アミノプロパンスルホン酸(TAPSO)、ピペラジン-N,N’-ビス（2-ヒドロキシプロパンスルホン酸）(POPSO)、3-〔4-（2-ヒドロキシエチル）-1-ピペラジニル〕-2-ヒドロキシプロパンスルホン酸(HEPPSO)、3-〔4-（2-ヒドロキシエチル）-1-ピペラジニル〕プロパンスルホン酸[(H)EPPS]、N-〔トリス（ヒドロキシメチル）メチル〕グリシン(Tricine)、N,N-ビス（2-ヒドロキシエチル）グリシン(Bicine)、N-トリス（ヒドロキシメチル）メチル-3-アミノプロパンスルホン酸(TAPS)、N-シクロヘキシル-2-アミノエタンスルホン酸(CHES)、N-シクロヘキシル-3-アミノ-2-ヒドロキシプロパンスルホン酸(CAPSO)、N-シクロヘキシル-3-アミノプロパンスルホン酸(CAPS)等が挙げられる。 As the diluent, any diluent can be used as long as it is used for diluting the specimen and enables accurate measurement of the measurement target substance in the specimen. For example, a protein, an antiseptic, a surfactant, etc. An aqueous medium containing Examples of proteins include bovine serum albumin and casein. Examples of the preservative include sodium azide, antibiotics (streptomycin, penicillin, gentamicin, etc.), bioace, procrine 300, and the like. Examples of the surfactant include a cationic surfactant, an anionic surfactant, an amphoteric surfactant, and a nonionic surfactant. Examples of the aqueous medium include deionized water, distilled water, and a buffer solution. Examples of the buffer used in the buffer include tris (hydroxymethyl) aminomethane buffer, phosphate buffer, borate buffer, Good's buffer, and the like. Good buffering agents include, for example, 2-morpholinoethanesulfonic acid (MES), bis (2-hydroxyethyl) iminotris (hydroxymethyl) methane (Bis-Tris), N- (2-acetamido) iminodiacetic acid (ADA) Piperazine-N, N'-bis (2-ethanesulfonic acid) (PIPES), N- (2-acetamido) -2-aminoethanesulfonic acid (ACES), 3-morpholino-2-hydroxypropanesulfonic acid (MOPSO) ), N, N-bis (2-hydroxyethyl) -2-aminoethanesulfonic acid (BES), 3-morpholinopropanesulfonic acid (MOPS), N- [tris (hydroxymethyl) methyl] -2-aminoethanesulfone Acid (TES), 2- [4- (2-hydroxyethyl) -1-piperazinyl] ethanesulfonic acid (HEPES), 3- [N, N-bis (2-hydroxyethyl) amino] -2-hydroxypropanesulfone Acid (DIPSO), N- [Tris (hydroxymethyl) methyl] -2-hydroxy-3- Minopropanesulfonic acid (TAPSO), piperazine-N, N'-bis (2-hydroxypropanesulfonic acid) (POPSO), 3- [4- (2-hydroxyethyl) -1-piperazinyl] -2-hydroxypropanesulfone Acid (HEPPSO), 3- [4- (2-hydroxyethyl) -1-piperazinyl] propanesulfonic acid [(H) EPPS], N- [tris (hydroxymethyl) methyl] glycine (Tricine), N, N- Bis (2-hydroxyethyl) glycine (Bicine), N-tris (hydroxymethyl) methyl-3-aminopropanesulfonic acid (TAPS), N-cyclohexyl-2-aminoethanesulfonic acid (CHES), N-cyclohexyl-3 -Amino-2-hydroxypropanesulfonic acid (CAPSO), N-cyclohexyl-3-aminopropanesulfonic acid (CAPS) and the like.
　図３に示すように、支持台２１０の天井部２１２の下面には、天井部２１２の上面から回転可能に回転軸２１８を突出させて、駆動手段としてのモータ２０６が取り付けられている。 As shown in FIG. 3, a motor 206 as a driving unit is attached to the lower surface of the ceiling portion 212 of the support base 210 by projecting a rotating shaft 218 from the upper surface of the ceiling portion 212.
　モータ２０６の回転軸２１８には、円板状の遮光部材２２０が固定され、この遮光部材２２０の上面には、支柱２２２、２２４が立設されている。さらに、支柱２２２、２２４の上部には、図２に示すように、容器の口部としての希釈液容器６４Ａ、６４Ｂ、６４Ｃの開口部２２６Ａ、２２６Ｂ、２２６Ｃを覆うように、扇状の板によって形成された蓋部材２０４が固定されている。希釈液容器６４Ａ、６４Ｂ、６４Ｃの開口部２２６Ａ、２２６Ｂ、２２６Ｃは、円形に形成されている。 A disk-shaped light shielding member 220 is fixed to the rotating shaft 218 of the motor 206, and supports 222 and 224 are erected on the upper surface of the light shielding member 220. Further, as shown in FIG. 2, the upper portions of the columns 222 and 224 are formed by fan-shaped plates so as to cover the openings 226A, 226B, and 226C of the diluent containers 64A, 64B, and 64C as the mouth portions of the containers. The lid member 204 is fixed. The openings 226A, 226B, and 226C of the diluent containers 64A, 64B, and 64C are formed in a circular shape.
　モータ２０６を回転することによって、モータ２０６の回転軸２１８を回転中心にして、遮光部材２２０を時計回り又は反時計回りの方向へ旋回させる。これに伴い、支柱２２２、２２４を介して遮光部材２２０と一体化されている蓋部材２０４が遮光部材２２０と連動し、モータ２０６の回転軸２１８を回転中心にして時計回り又は反時計回りの方向へ旋回して移動する。そして、この蓋部材２０４の旋回による移動によって、希釈液容器６４Ａ、６４Ｂ、６４Ｃの開口部２２６Ａ、２２６Ｂ、２２６Ｃを開閉する。 Rotating the motor 206 causes the light shielding member 220 to turn clockwise or counterclockwise around the rotation shaft 218 of the motor 206 as a rotation center. Accordingly, the lid member 204 integrated with the light shielding member 220 via the support columns 222 and 224 is interlocked with the light shielding member 220, and rotates clockwise or counterclockwise around the rotation shaft 218 of the motor 206. Turn to move. Then, the opening portions 226A, 226B, and 226C of the diluent containers 64A, 64B, and 64C are opened and closed by the movement of the lid member 204 by turning.
　また、図３に示すように、支持台２１０の天井部２１２の上面には、蓋部材２０４が反時計回りの方向へ旋回し過ぎた場合に、この旋回を阻止する棒状のストッパー部材２２８が固定されている。さらに、支持台２１０の天井部２１２の上面には、蓋部材２０４が時計回りの方向へ旋回し過ぎた場合に、この旋回を阻止する棒状のストッパー部材２３０が固定されている。説明の都合上、図２には、ストッパー部２２８、２３０が省略されている。 Further, as shown in FIG. 3, a rod-like stopper member 228 is fixed to the upper surface of the ceiling portion 212 of the support base 210 when the lid member 204 is turned in the counterclockwise direction. Has been. Further, a bar-shaped stopper member 230 is fixed to the upper surface of the ceiling portion 212 of the support base 210 to prevent the lid member 204 from pivoting in the clockwise direction. For convenience of explanation, the stopper portions 228 and 230 are omitted in FIG.
　図２及び図３に示すように、蓋部材２０４には、希釈液容器６４Ａ、６４Ｂの開口部２２６Ａ、２２６Ｂへの液体吸引部材としての分注ノズル８６の挿入を許容する、開口部２２６Ａ、２２６Ｂと略等しい大きさの２つの穴２３２Ａ、２３２Ｂが形成されている。すなわち、２つの穴２３２Ａ、２３２Ｂは、３つの希釈液容器６４Ａ、６４Ｂ、６４Ｃの数よりも１つ少なく形成されている。２つの穴２３２Ａ、２３２Ｂは、反応テーブル１６側から検体テーブル１８側へ向かってこの順に蓋部材２０４に形成されている。 As shown in FIGS. 2 and 3, the lid member 204 has openings 226A and 226B that allow the dispensing nozzle 86 as a liquid suction member to be inserted into the openings 226A and 226B of the diluent containers 64A and 64B. Two holes 232A and 232B having substantially the same size are formed. That is, the two holes 232A and 232B are formed by one less than the number of the three diluent containers 64A, 64B and 64C. The two holes 232A and 232B are formed in the lid member 204 in this order from the reaction table 16 side to the sample table 18 side.
　また、穴２３２Ａ、２３２Ｂは、穴２３２Ａを希釈液容器６４Ａの開口部２２６Ａと略一致させたときに、穴２３２Ｂが希釈液容器６４Ｂの開口部２２６Ｂと略一致するように配置されている。また、穴２３２Ａ、２３２Ｂは、蓋部材２０４の旋回による穴２３２Ａ、２３２Ｂの中心の旋回軌道と、円周２１６とがずれるように配置されている。 The holes 232A and 232B are arranged so that the hole 232B substantially coincides with the opening 226B of the diluent container 64B when the hole 232A is substantially coincident with the opening 226A of the diluent container 64A. Further, the holes 232A and 232B are arranged so that the turning trajectory at the center of the holes 232A and 232B due to the turning of the lid member 204 and the circumference 216 are shifted.
　図２及び図４Ｂのように配置された蓋部材２０４によって覆われた希釈液容器６４Ａ、６４Ｂ、６４Ｃの少なくとも１つから希釈液を吸引するときには、図４Ａの平面図に示すように、図４Ｂの状態からモータ２０６を駆動し容器設置部２０２Ａ、２０２Ｂ、２０２Ｃ（希釈液容器６４Ａ、６４Ｂ、６４Ｃ）の上方で蓋部材２０４を時計回りの方向へ旋回し移動させる。そして、この移動により、検体テーブル１８側の最外側に配置されている希釈液容器６４Ｃ以外の全ての希釈液容器６４Ａ、６４Ｂの開口部２２６Ａ、２２６Ｂと、穴２３２Ａ、２３２Ｂとを略一致させる。また、このとき、検体テーブル１８側の最外側に配置されている希釈液容器６４Ｃの開口部２２６Ｃを覆わないように蓋部材２０４を配置する。これによって、容器設置部２０２Ａ、２０２Ｂ、２０２Ｃに並べられた全ての希釈液容器６４Ａ、６４Ｂ、６４Ｃの開口部２２６Ａ、２２６Ｂ、２２６Ｃを開放状態にする。このときの蓋部材２０４の位置を容器開放位置とする。 When sucking the diluent from at least one of the diluent containers 64A, 64B, 64C covered by the lid member 204 arranged as shown in FIGS. 2 and 4B, as shown in the plan view of FIG. In this state, the motor 206 is driven to rotate and move the lid member 204 in the clockwise direction above the container setting portions 202A, 202B, 202C ( diluent containers 64A, 64B, 64C). As a result of this movement, the openings 226A and 226B of all the diluent containers 64A and 64B other than the diluent container 64C arranged on the outermost side on the specimen table 18 side and the holes 232A and 232B are substantially matched. At this time, the lid member 204 is disposed so as not to cover the opening 226C of the diluent container 64C disposed on the outermost side on the specimen table 18 side. Thereby, the openings 226A, 226B, and 226C of all the diluent containers 64A, 64B, and 64C arranged in the container setting portions 202A, 202B, and 202C are opened. The position of the lid member 204 at this time is defined as a container opening position.
　また、ロボットアーム８０の旋回により、検体が吸引された分注ノズル８６を希釈液容器６４Ａ、６４Ｂ、６４Ｃの上方で移動させるときには、図４Ｂの平面図に示すように、図４Ａの状態からモータ２０６を駆動し容器設置部２０２Ａ、２０２Ｂ、２０２Ｃ（希釈液容器６４Ａ、６４Ｂ、６４Ｃ）の上方で蓋部材２０４を反時計回りの方向へ旋回し移動させる。そして、この移動により、希釈液容器６４Ａ、６４Ｂの開口部２２６Ａ、２２６Ｂから穴２３２Ａ、２３２Ｂを退避させる。これによって、容器設置部２０２Ａ、２０２Ｂ、２０２Ｃに並べられた全ての希釈液容器６４Ａ、６４Ｂ、６４Ｃの開口部２２６Ａ、２２６Ｂ、２２６Ｃを蓋部材２０４で覆い閉止状態にする。このときの蓋部材２０４の位置を容器閉止位置とする。 When the dispensing nozzle 86 from which the specimen has been aspirated is moved above the diluent containers 64A, 64B, and 64C by turning the robot arm 80, the motor is moved from the state of FIG. 4A as shown in the plan view of FIG. 4B. 206 is driven, and the lid member 204 is swung in the counterclockwise direction and moved above the container setting portions 202A, 202B, 202C ( diluent containers 64A, 64B, 64C). By this movement, the holes 232A and 232B are retracted from the openings 226A and 226B of the diluent containers 64A and 64B. As a result, the openings 226A, 226B, and 226C of all the diluent containers 64A, 64B, and 64C arranged in the container setting portions 202A, 202B, and 202C are covered with the lid member 204 to be closed. The position of the lid member 204 at this time is set as a container closing position.
　さらに、希釈液容器６４Ａ、６４Ｂ、６４Ｃを交換するときには、図４Ｃの平面図に示すように、図４Ａ又は図４Ｂの状態からモータ２０６を駆動し容器設置部２０２Ａ、２０２Ｂ、２０２Ｃ（希釈液容器６４Ａ、６４Ｂ、６４Ｃ）の上方で蓋部材２０４を時計回りの方向へ旋回し移動させる。そして、この移動により、容器設置部２０２Ａ、２０２Ｂ、２０２Ｃに並べられた全ての希釈液容器６４Ａ、６４Ｂ、６４Ｃの上方から蓋部材２０４を退避させた状態にする。このときの蓋部材２０４の位置を容器交換位置とする。 Further, when exchanging the diluent containers 64A, 64B, 64C, as shown in the plan view of FIG. 4C, the motor 206 is driven from the state of FIG. 4A or FIG. 64A, 64B, 64C), the lid member 204 is pivoted and moved in the clockwise direction. By this movement, the lid member 204 is retracted from above the diluent containers 64A, 64B, and 64C arranged in the container setting sections 202A, 202B, and 202C. The position of the lid member 204 at this time is set as a container replacement position.
　図３、及び図５Ａの平面図に示すように、蓋部材２０４の位置は、支持台２１０の天井部２１２の上面に設置された２つの光電センサ２３４、２３６と、遮光部材２２０とによって検知する。 As shown in the plan views of FIGS. 3 and 5A, the position of the lid member 204 is detected by the two photoelectric sensors 234 and 236 installed on the upper surface of the ceiling portion 212 of the support base 210 and the light shielding member 220. .
　光電センサ２３４、２３６は、遮光部材２２０の回転中心２３８（回転軸２１８）に対して点対称となる位置に配置されている。また、遮光部材２２０には、遮光部２４０、２４２、２４４と、切り欠き部２４６、２４８、２５０とが遮光部材２２０の周方向へ交互に配置されている。 The photoelectric sensors 234 and 236 are arranged at positions that are point-symmetric with respect to the rotation center 238 (rotation axis 218) of the light shielding member 220. In the light shielding member 220, light shielding portions 240, 242, 244 and notches 246, 248, 250 are alternately arranged in the circumferential direction of the light shielding member 220.
　図６Ａの斜視図に示すように、光電センサ２３４、２３６の投光部２５２と受光部２５４との間に遮光部２４０、２４２が配置されたときには、投光部２５２から発せられた光は、遮光部２４０、２４２によって遮光されて受光部２５４によって受光されない。以下、このことを「光電センサ２３４、２３６の受光部２５４が遮光状態にある」とする。 As shown in the perspective view of FIG. 6A, when the light shielding units 240 and 242 are disposed between the light projecting unit 252 and the light receiving unit 254 of the photoelectric sensors 234 and 236, the light emitted from the light projecting unit 252 is The light is blocked by the light blocking portions 240 and 242 and is not received by the light receiving portion 254. Hereinafter, this is referred to as “the light receiving portions 254 of the photoelectric sensors 234 and 236 are in a light shielding state”.
　また、図６Ｂ及び図６Ｃの斜視図に示すように、光電センサ２３４、２３６の投光部２５２と受光部２５４との間に切り欠き部２４６、２４８、２５０が配置されたときには、投光部２５２から発せられた光は、切り欠き部２４６、２４８、２５０を通過して受光部２５４により受光される。以下、このことを「光電センサ２３４、２３６の受光部２５４が入光状態にある」とする。 6B and 6C, when the notches 246, 248, 250 are arranged between the light projecting unit 252 and the light receiving unit 254 of the photoelectric sensors 234, 236, the light projecting unit The light emitted from 252 passes through the notches 246, 248, 250 and is received by the light receiver 254. Hereinafter, this is referred to as “the light receiving portions 254 of the photoelectric sensors 234 and 236 are in a light incident state”.
　図５Ａに示すように、図４Ａで示した容器開放位置に蓋部材２０４が配置されているときには、光電センサ２３４の投光部２５２と受光部２５４との間に切り欠き部２４６が配置され、光電センサ２３６の投光部２５２と受光部２５４との間に切り欠き部２５０が配置される。そして、光電センサ２３４の投光部２５２と受光部２５４との間に切り欠き部２４６が配置されることにより、光電センサ２３４の受光部２５４は入光状態にある。また、光電センサ２３６の投光部２５２と受光部２５４との間に切り欠き部２５０が配置されることにより、光電センサ２３６の受光部２５４は入光状態にある。 As shown in FIG. 5A, when the lid member 204 is arranged at the container opening position shown in FIG. 4A, a notch 246 is arranged between the light projecting unit 252 and the light receiving unit 254 of the photoelectric sensor 234, A notch 250 is disposed between the light projecting unit 252 and the light receiving unit 254 of the photoelectric sensor 236. Then, the notch 246 is disposed between the light projecting unit 252 and the light receiving unit 254 of the photoelectric sensor 234, so that the light receiving unit 254 of the photoelectric sensor 234 is in a light incident state. Further, the notch portion 250 is disposed between the light projecting portion 252 and the light receiving portion 254 of the photoelectric sensor 236, so that the light receiving portion 254 of the photoelectric sensor 236 is in a light incident state.
　図５Ｂに示すように、図４Ｂで示した容器閉止位置に蓋部材２０４が配置されているときには、光電センサ２３４の投光部２５２と受光部２５４との間に遮光部２４２が配置され、光電センサ２３６の投光部２５２と受光部２５４との間に遮光部２４０が配置される。そして、光電センサ２３４の投光部２５２と受光部２５４との間に遮光部２４２が配置されることにより、光電センサ２３４の受光部２５４は遮光状態にある。また、光電センサ２３６の投光部２５２と受光部２５４との間に遮光部２４０が配置されることにより、光電センサ２３６の受光部２５４は遮光状態にある。 As shown in FIG. 5B, when the lid member 204 is arranged at the container closing position shown in FIG. 4B, the light shielding unit 242 is arranged between the light projecting unit 252 and the light receiving unit 254 of the photoelectric sensor 234, A light shielding unit 240 is disposed between the light projecting unit 252 and the light receiving unit 254 of the sensor 236. Then, the light shielding unit 242 is disposed between the light projecting unit 252 and the light receiving unit 254 of the photoelectric sensor 234, whereby the light receiving unit 254 of the photoelectric sensor 234 is in a light shielding state. In addition, since the light shielding unit 240 is disposed between the light projecting unit 252 and the light receiving unit 254 of the photoelectric sensor 236, the light receiving unit 254 of the photoelectric sensor 236 is in a light shielding state.
　図５Ｃに示すように、図４Ｃで示した容器交換位置に蓋部材２０４が配置されているときには、光電センサ２３４の投光部２５２と受光部２５４との間に遮光部２４０が配置され、光電センサ２３６の投光部２５２と受光部２５４との間に切り欠き部２４８が配置される。そして、光電センサ２３４の投光部２５２と受光部２５４との間に遮光部２４０が配置されることにより、光電センサ２３４の受光部２５４は遮光状態にある。また、光電センサ２３６の投光部２５２と受光部２５４との間に切り欠き部２４８が配置されることにより、光電センサ２３６の受光部２５４は入光状態にある。 As shown in FIG. 5C, when the lid member 204 is arranged at the container replacement position shown in FIG. 4C, the light shielding unit 240 is arranged between the light projecting unit 252 and the light receiving unit 254 of the photoelectric sensor 234, A notch 248 is disposed between the light projecting unit 252 and the light receiving unit 254 of the sensor 236. Then, by arranging the light shielding unit 240 between the light projecting unit 252 and the light receiving unit 254 of the photoelectric sensor 234, the light receiving unit 254 of the photoelectric sensor 234 is in a light shielding state. Further, the notch portion 248 is disposed between the light projecting portion 252 and the light receiving portion 254 of the photoelectric sensor 236, so that the light receiving portion 254 of the photoelectric sensor 236 is in a light incident state.
　すなわち、光電センサ２３４の受光部２５４が入光状態にあり、光電センサ２３６の受光部２５４も入光状態にあるときに、蓋部材２０４は容器開放位置に配置されていると判断できる。また、光電センサ２３４の受光部２５４が遮光状態にあり、光電センサ２３６の受光部２５４も遮光状態にあるときに、蓋部材２０４は容器閉止位置に配置されていると判断できる。さらに、光電センサ２３４の受光部２５４が遮光状態にあり、光電センサ２３６の受光部２５４が入光状態にあるときに、蓋部材２０４は容器交換位置に配置されていると判断できる。このようにして、蓋部材２０４の位置を検知する。 That is, when the light receiving unit 254 of the photoelectric sensor 234 is in the light incident state and the light receiving unit 254 of the photoelectric sensor 236 is also in the light incident state, it can be determined that the lid member 204 is disposed at the container open position. Further, when the light receiving unit 254 of the photoelectric sensor 234 is in a light shielding state and the light receiving unit 254 of the photoelectric sensor 236 is also in a light shielding state, it can be determined that the lid member 204 is disposed at the container closing position. Furthermore, when the light receiving unit 254 of the photoelectric sensor 234 is in a light shielding state and the light receiving unit 254 of the photoelectric sensor 236 is in a light entering state, it can be determined that the lid member 204 is disposed at the container replacement position. In this way, the position of the lid member 204 is detected.
　なお、切り欠き部及び遮光部の配置や、蓋部材２０４の位置（容器開放位置、容器閉止位置、容器交換位置）に対する受光部の遮光状態と入光状態との組み合わせは、この例に限らずに、適宜決めればよい。 Note that the arrangement of the cutout portion and the light shielding portion, and the combination of the light shielding portion and the light incident state of the light receiving portion with respect to the position of the lid member 204 (container opening position, container closing position, container replacement position) are not limited to this example. It may be determined as appropriate.
＜作用＞
　本実施形態に係る希釈液保管ユニット２０の作用について説明する。図４Ａに示すように、検体テーブル１８側の最外側に配置されている希釈液容器６４Ｃ以外の全ての希釈液容器６４Ａ、６４Ｂの開口部２２６Ａ、２２６Ｂと、穴２３２Ａ、２３２Ｂとを略一致させたときに、容器設置部２０２Ａ、２０２Ｂ、２０２Ｃに並べられた全ての希釈液容器６４Ａ、６４Ｂ、６４Ｃの開口部２２６Ａ、２２６Ｂ、２２６Ｃが開放される。これによって、これらの開口部２２６Ａ、２２６Ｂ、２２６Ｃへ液体吸引部材としての分注ノズル８６を挿入することができ、これらの希釈液容器６４Ａ、６４Ｂ、６４Ｃからの希釈液吸引が可能になる。 <Action>
The operation of the diluent storage unit 20 according to this embodiment will be described. As shown in FIG. 4A, the openings 226A and 226B of all the diluent containers 64A and 64B other than the diluent container 64C arranged on the outermost side on the specimen table 18 side are made to substantially coincide with the holes 232A and 232B. When this happens, the openings 226A, 226B, and 226C of all the diluent containers 64A, 64B, and 64C arranged in the container setting portions 202A, 202B, and 202C are opened. As a result, a dispensing nozzle 86 as a liquid suction member can be inserted into these openings 226A, 226B, and 226C, and dilution liquid suction from these dilution liquid containers 64A, 64B, and 64C becomes possible.
　そして、このときに蓋部材２０４は、希釈液容器６４Ａ、６４Ｂ、６４Ｃの開口部２２６Ａ、２２６Ｂ、２２６Ｃが閉止されている容器閉止位置(図４Ｂに示されている蓋部材２０４の位置）から、蓋部材２０４の穴２３２Ａ、２３２Ｂが希釈液容器６４Ａ、６４Ｂの開口部２２６Ａ、２２６Ｂと略一致する容器開放位置(図４Ａに示されている蓋部材２０４の位置）まで移動させればよい。よって、蓋部材２０４の少ない移動距離で希釈液容器６４Ａ、６４Ｂ、６４Ｃの開口部２２６Ａ、２２６Ｂ、２２６Ｃを開放することができる。すなわち、短い時間で希釈液容器６４Ａ、６４Ｂ、６４Ｃの開口部２２６Ａ、２２６Ｂ、２２６Ｃを開放することができる。 At this time, the lid member 204 is moved from the container closing position (the position of the lid member 204 shown in FIG. 4B) where the openings 226A, 226B, and 226C of the diluent containers 64A, 64B, and 64C are closed. The holes 232A and 232B of the lid member 204 may be moved to the container opening position (the position of the lid member 204 shown in FIG. 4A) that substantially coincides with the openings 226A and 226B of the diluent containers 64A and 64B. Therefore, the openings 226A, 226B, and 226C of the diluent containers 64A, 64B, and 64C can be opened with a small moving distance of the lid member 204. That is, the openings 226A, 226B, and 226C of the diluent containers 64A, 64B, and 64C can be opened in a short time.
　例えば、図４Ｂに示すように、蓋部材２０４が容器閉止位置に配置されている状態で、隣り合う希釈液容器６４Ａの開口部２２６Ａと、希釈液容器６４Ｂの開口部２２６Ｂとの中間に穴２３２Ａが配置され、隣り合う希釈液容器６４Ｂの開口部２２６Ｂと、希釈液容器６４Ｃの開口部２２６Ｃとの中間に穴２３２Ｂが配置されるようになっている場合には、希釈液容器６４Ａの開口部２２６Ａと、希釈液容器６４Ｂの開口部２２６Ｂとの中心間距離の約半分の距離（希釈液容器６４Ｂの開口部２２６Ｂと、希釈液容器６４Ｃの開口部２２６Ｃとの中心間距離の約半分の距離）だけ、蓋部材２０４を移動させることにより、希釈液容器６４Ａ、６４Ｂ、６４Ｃの開口部２２６Ａ、２２６Ｂ、２２６Ｃを開放することができる。 For example, as shown in FIG. 4B, in a state where the lid member 204 is disposed at the container closing position, a hole 232A is provided between the opening 226A of the adjacent diluent container 64A and the opening 226B of the diluent container 64B. Is arranged, and the hole 232B is arranged between the opening 226B of the adjacent diluent container 64B and the opening 226C of the diluent container 64C, the opening of the diluent container 64A The distance between the centers of 226A and the opening 226B of the diluent container 64B is about half the distance (the distance between the centers of the openings 226B of the diluent container 64B and the opening 226C of the diluent container 64C is about half. ), The openings 226A, 226B, and 226C of the diluent containers 64A, 64B, and 64C can be opened by moving the lid member 204.
　また、検体テーブル１８側の最外側に配置されている希釈液容器６４Ｃ以外の全ての希釈液容器６４Ａ、６４Ｂの開口部２２６Ａ、２２６Ｂと、穴２３２Ａ、２３２Ｂとを略一致させ、検体テーブル１８側の最外側に配置されている希釈液容器６４Ｃの開口部２２６Ｃを蓋部材２０４により覆わないことによって、容器設置部２０２Ａ、２０２Ｂ、２０２Ｃに並べられた全ての希釈液容器６４Ａ、６４Ｂ、６４Ｃの開口部２２６Ａ、２２６Ｂ、２２６Ｃを開放する。これにより、蓋部材２０４のサイズを小さくすることができる。 Further, the openings 226A and 226B of all the diluent containers 64A and 64B other than the diluent container 64C arranged on the outermost side on the sample table 18 side are substantially matched with the holes 232A and 232B, so that the sample table 18 side By not covering the opening 226C of the diluent container 64C disposed on the outermost side with the lid member 204, the openings of all the diluent containers 64A, 64B, and 64C arranged in the container installation portions 202A, 202B, and 202C The parts 226A, 226B, and 226C are opened. Thereby, the size of the lid member 204 can be reduced.
　さらに、図４Ｂに示すように、検体テーブル１８側の最外側に配置されている希釈液容器６４Ｃ以外の全ての希釈液容器６４Ａ、６４Ｂの開口部２２６Ａ、２２６Ｂから穴２３２Ａ、２３２Ｂをずらし、容器設置部２０２Ａ、２０２Ｂ、２０２Ｃに並べられた全ての希釈液容器６４Ａ、６４Ｂ、６４Ｃの開口部２２６Ａ、２２６Ｂ、２２６Ｃを蓋部材２０４で覆うことによって、これらの希釈液容器６４Ａ、６４Ｂ、６４Ｃの開口部２２６Ａ、２２６Ｂ、２２６Ｃを閉止することができる。 Further, as shown in FIG. 4B, the holes 232A and 232B are shifted from the openings 226A and 226B of all the diluent containers 64A and 64B other than the diluent container 64C arranged on the outermost side on the specimen table 18 side. By covering the openings 226A, 226B, and 226C of all the diluent containers 64A, 64B, and 64C arranged in the installation sections 202A, 202B, and 202C with the lid member 204, the openings of these diluent containers 64A, 64B, and 64C are covered. The parts 226A, 226B, 226C can be closed.
　そして、このときに蓋部材２０４は、蓋部材２０４の穴２３２Ａ、２３２Ｂが希釈液容器６４Ａ、６４Ｂの開口部２２６Ａ、２２６Ｂと略一致する容器開放位置(図４Ａに示されている蓋部材２０４の位置）から、希釈液容器６４Ａ、６４Ｂ、６４Ｃの開口部２２６Ａ、２２６Ｂ、２２６Ｃが閉止されている容器閉止位置(図４Ｂに示されている蓋部材２０４の位置）まで移動させればよい。よって、蓋部材２０４の少ない移動距離で希釈液容器６４Ａ、６４Ｂ、６４Ｃの開口部２２６Ａ、２２６Ｂ、２２６Ｃを閉止することができる。すなわち、短い時間で希釈液容器６４Ａ、６４Ｂ、６４Ｃの開口部２２６Ａ、２２６Ｂ、２２６Ｃを閉止することができる。 At this time, the lid member 204 has a container open position where the holes 232A and 232B of the lid member 204 substantially coincide with the openings 226A and 226B of the diluent containers 64A and 64B (the lid member 204 of the lid member 204 shown in FIG. 4A). Position) to the container closing position where the openings 226A, 226B, and 226C of the diluent containers 64A, 64B, and 64C are closed (the position of the lid member 204 shown in FIG. 4B). Therefore, the openings 226A, 226B, and 226C of the diluent containers 64A, 64B, and 64C can be closed with a small moving distance of the lid member 204. That is, the openings 226A, 226B, and 226C of the diluent containers 64A, 64B, and 64C can be closed in a short time.
　例えば、図４Ｂに示すように、蓋部材２０４が容器閉止位置に配置されている状態で、隣り合う希釈液容器６４Ａの開口部２２６Ａと、希釈液容器６４Ｂの開口部２２６Ｂとの中間に穴２３２Ａが配置され、隣り合う希釈液容器６４Ｂの開口部２２６Ｂと、希釈液容器６４Ｃの開口部２２６Ｃとの中間に穴２３２Ｂが配置されるようになっている場合には、希釈液容器６４Ａの開口部２２６Ａと、希釈液容器６４Ｂの開口部２２６Ｂとの中心間距離の約半分の距離（希釈液容器６４Ｂの開口部２２６Ｂと、希釈液容器６４Ｃの開口部２２６Ｃとの中心間距離の約半分の距離）だけ、蓋部材２０４を移動させればよい。 For example, as shown in FIG. 4B, in a state where the lid member 204 is disposed at the container closing position, a hole 232A is provided between the opening 226A of the adjacent diluent container 64A and the opening 226B of the diluent container 64B. Is arranged, and the hole 232B is arranged between the opening 226B of the adjacent diluent container 64B and the opening 226C of the diluent container 64C, the opening of the diluent container 64A The distance between the centers of 226A and the opening 226B of the diluent container 64B is about half the distance (the distance between the centers of the openings 226B of the diluent container 64B and the opening 226C of the diluent container 64C is about half. Only the lid member 204 needs to be moved.
　さらに、図４Ｃに示すように、容器設置部２０２Ａ、２０２Ｂ、２０２Ｃに並べられた全ての希釈液容器６４Ａ、６４Ｂ、６４Ｃの上方から蓋部材２０４を退避させることによって、希釈液容器６４Ａ、６４Ｂ、６４Ｃの入れ替えをスムーズに行うことができる。 Further, as shown in FIG. 4C, the diluent members 64A, 64B, 64B, 64B, 64B, 64B, The replacement of 64C can be performed smoothly.
　また、図４Ａに示すように、蓋部材２０４に形成する穴２３２Ａ、２３２Ｂの数は、希釈液容器６４Ａ、６４Ｂ、６４Ｃの数よりも１つ少ない。よって、少ない数の穴２３２Ａ、２３２Ｂで、容器設置部２０２Ａ、２０２Ｂ、２０２Ｃに並べられた全ての希釈液容器６４Ａ、６４Ｂ、６４Ｃの開口部２２６Ａ、２２６Ｂ、２２６Ｃを開閉することができる。 As shown in FIG. 4A, the number of holes 232A, 232B formed in the lid member 204 is one less than the number of diluent containers 64A, 64B, 64C. Therefore, the openings 226A, 226B, and 226C of all the diluent containers 64A, 64B, and 64C arranged in the container setting portions 202A, 202B, and 202C can be opened and closed with a small number of holes 232A and 232B.
　さらに、図２に示すように、蓋部材２０４により、容器設置部２０２Ａ、２０２Ｂ、２０２Ｃ内にセットされて並べられた全ての希釈液容器６４Ａ、６４Ｂ、６４Ｃの開口部２２６Ａ、２２６Ｂ、２２６Ｃを閉止することによって、分注装置３０の分注ノズル８６から希釈液容器６４Ａ、６４Ｂ、６４Ｃ内に検体が落下混入するのを防ぐことができる。これにより、希釈液容器６４Ａ、６４Ｂ、６４Ｃ内に収容されている希釈液のコンタミネーションを防ぐことができる。希釈液容器６４Ａ、６４Ｂ、６４Ｃは、分注ノズル８６の旋回軌道上に配置されているので、希釈液のコンタミネーションの危険性が強くなる。よって、本実施形態の容器開閉装置２００の適用が特に有効になる。 Further, as shown in FIG. 2, the lid member 204 closes the openings 226A, 226B, and 226C of all the diluent containers 64A, 64B, and 64C that are set and arranged in the container setting portions 202A, 202B, and 202C. By doing so, it is possible to prevent the specimen from falling into the diluent container 64A, 64B, 64C from the dispensing nozzle 86 of the dispensing apparatus 30. Thereby, contamination of the diluent stored in the diluent containers 64A, 64B, 64C can be prevented. Since the dilution liquid containers 64A, 64B, and 64C are arranged on the swirling trajectory of the dispensing nozzle 86, the risk of contamination of the dilution liquid increases. Therefore, application of the container opening / closing device 200 of the present embodiment is particularly effective.
　また、図２に示すように、容器設置部２０２Ａ、２０２Ｂ、２０２Ｃにセットされて並べられた全ての希釈液容器６４Ａ、６４Ｂ、６４Ｃの開口部２２６Ａ、２２６Ｂ、２２６Ｃの開閉は、蓋部材２０４の旋回移動によって行う。これによって、蓋部材２０４を直線移動させて、これらの希釈液容器６４Ａ、６４Ｂ、６４Ｃの開口部２２６Ａ、２２６Ｂ、２２６Ｃを開閉させる場合と比較して、簡単な機構で効率よく蓋部材２０４を移動させることができる。 Further, as shown in FIG. 2, opening and closing of the openings 226A, 226B, and 226C of all the diluent containers 64A, 64B, and 64C set and arranged in the container setting portions 202A, 202B, and 202C are performed on the lid member 204. This is done by turning movement. As a result, the lid member 204 is moved linearly, and the lid member 204 is efficiently moved with a simple mechanism as compared with the case where the openings 226A, 226B, and 226C of the diluent containers 64A, 64B, and 64C are opened and closed. Can be made.
　さらに、図２に示すように、穴２３２Ａ、２３２Ｂは、蓋部材２０４の旋回による穴２３２Ａ、２３２Ｂの中心の旋回軌道と、円周２１６とがずれるように配置されている。これによって、蓋部材２０４が容器閉止位置にあるときに、円周２１６の半径方向内側または外側へずれた位置に穴２３２Ａ、２３２Ｂが配置される。よって、円周２１６上における、隣り合う希釈液容器６４Ａ、６４Ｂ、６４Ｃの開口部２２６Ａ、２２６Ｂ、２２６Ｃ同士の離れている距離を穴２３２Ａ、２３２Ｂの直径よりも小さくすることができる。すなわち、希釈液容器６４Ａ、６４Ｂ、６４Ｃの設置間隔を狭くすることができる。 Further, as shown in FIG. 2, the holes 232A and 232B are arranged so that the turning trajectory at the center of the holes 232A and 232B due to the turning of the lid member 204 and the circumference 216 are shifted. As a result, when the lid member 204 is in the container closing position, the holes 232A and 232B are arranged at positions shifted inward or outward in the radial direction of the circumference 216. Therefore, the distance between the openings 226A, 226B, and 226C of the adjacent diluent containers 64A, 64B, and 64C on the circumference 216 can be made smaller than the diameter of the holes 232A and 232B. That is, the installation interval of the diluent containers 64A, 64B, 64C can be reduced.
　なお、本実施形態では、希釈液容器６４Ａ、６４Ｂ、６４Ｃのそれぞれに、異なる種類の希釈液を収容させている例を示したが、希釈液容器６４Ａ、６４Ｂ、６４Ｃのそれぞれに、同じ種類の希釈液が収容されていてもよい。 In the present embodiment, an example is shown in which different types of diluents are stored in each of the diluent containers 64A, 64B, and 64C. However, the same type of diluent is stored in each of the diluent containers 64A, 64B, and 64C. A diluent may be accommodated.
　また、本実施形態では、容器設置部２０２Ａ、２０２Ｂ、２０２Ｃに３つの希釈液容器６４Ａ、６４Ｂ、６４Ｃを並べた例を示したが、希釈液容器は、２つ以上並べられていればよい。この場合、蓋部材２０４に形成する穴の数は、希釈液容器の数よりも１つ少なくする。 In the present embodiment, an example is shown in which three diluent containers 64A, 64B, and 64C are arranged in the container setting sections 202A, 202B, and 202C. However, two or more diluent containers may be arranged. In this case, the number of holes formed in the lid member 204 is one less than the number of diluent containers.
　また、蓋部材２０４は、容器設置部２０２Ａ、２０２Ｂ、２０２Ｃにセットされて並べられた全ての希釈液容器６４Ａ、６４Ｂ、６４Ｃの開口部２２６Ａ、２２６Ｂ、２２６Ｃの開閉ができれば、どのような形状であってもよい。 The lid member 204 can have any shape as long as it can open and close the openings 226A, 226B, and 226C of all the diluent containers 64A, 64B, and 64C set and arranged in the container setting portions 202A, 202B, and 202C. There may be.
　さらに、本実施形態では、希釈液が収容されている希釈液容器６４Ａ、６４Ｂ、６４Ｃを容器とした例を示したが、他の液体が収容されている容器に対しても本実施形態の容器開閉装置２００を適用することができる。 Furthermore, in the present embodiment, an example is shown in which the diluent containers 64A, 64B, and 64C containing the diluent are used as containers. However, the container of the present embodiment is also applied to containers containing other liquids. The switchgear 200 can be applied.
　さらに、本実施形態では、３試薬（ストレプトアビジン結合磁性担体粒子含有試薬、ビオチン化一次抗体含有試薬、アルカリホスファターゼ標識抗体含有試薬）を用いる免疫測定法のための免疫測定装置１０に、容器開閉装置２００を適用した例を示したが、これに限らない。本実施形態は、前記３試薬を用いる以外の免疫測定法のための免疫測定装置や、免疫測定装置以外の測定装置に適用することができる。すなわち、本実施形態は、容器の口部の開閉を必要とするさまざまな用途の装置に適用することができる。 Furthermore, in this embodiment, the container opening / closing device is used in an immunoassay device 10 for immunoassay using three reagents (a reagent containing streptavidin-binding magnetic carrier particles, a reagent containing a biotinylated primary antibody, and a reagent containing an alkaline phosphatase-labeled antibody). Although an example in which 200 is applied has been shown, the present invention is not limited to this. This embodiment can be applied to an immunoassay apparatus for an immunoassay other than using the three reagents, or a measurement apparatus other than the immunoassay apparatus. In other words, the present embodiment can be applied to devices for various uses that require opening and closing of the mouth of the container.
　以上、本発明の実施形態について説明したが、本発明はこうした実施形態に限定されるものでなく、本発明の要旨を逸脱しない範囲において、種々なる態様で実施し得ることは勿論である。 As mentioned above, although embodiment of this invention was described, this invention is not limited to such embodiment, Of course, in the range which does not deviate from the summary of this invention, it can implement in a various aspect.
６４Ａ、６４Ｂ、６４Ｃ　希釈液容器（容器）
８６　分注ノズル（液体吸引部材）
２００　容器開閉装置
２０２Ａ、２０２Ｂ、２０２Ｃ　容器設置部
２０４　蓋部材
２０６　モータ（駆動手段）
２２６Ａ、２２６Ｂ、２２６Ｃ　開口部（口部）
２３２Ａ、２３２Ｂ　穴 64A, 64B, 64C Diluent container (container)
86 Dispensing nozzle (liquid suction member)
200 Container open / close device 202A, 202B, 202C Container installation unit 204 Lid member 206 Motor (drive means)
226A, 226B, 226C Opening (mouth)
232A, 232B hole

Claims (4)

  1. 　容器が並べられる複数の容器設置部と、
    　前記容器の口部を覆う蓋部材と、
    　前記蓋部材を移動させ前記容器の口部を開閉させる駆動手段と、
    　を備え、
    　前記蓋部材には、前記容器の口部への液体吸引部材の挿入を許容する穴が前記容器設置部の数よりも１つ少なく形成され、最外側以外の全ての前記容器の口部と前記穴とを一致させたとき、最外側の前記容器の口部は前記蓋部材に覆われず、前記容器の口部から前記穴を退避させたとき、前記容器設置部に並べられた全ての前記容器の口部が前記蓋部材で覆われるように、前記穴が配置されている容器開閉装置。     A plurality of container placement sections in which containers are arranged;
    A lid member covering the mouth of the container;
    Driving means for moving the lid member to open and close the mouth of the container;
    With
    The lid member is formed with a hole that allows insertion of the liquid suction member into the mouth portion of the container, one less than the number of the container installation portions, and the mouth portions of all the containers other than the outermost side and the When the holes are matched, the outermost mouth part of the container is not covered with the lid member, and when the hole is retracted from the mouth part of the container, A container opening and closing device in which the hole is arranged so that the mouth of the container is covered with the lid member.
  2. 　前記容器設置部は、円周上に配置され、
    　前記駆動手段は、前記容器設置部の上方で前記蓋部材を旋回させて移動させる請求項１に記載の容器開閉装置。     The container installation part is arranged on the circumference,
    The container opening and closing device according to claim 1, wherein the driving means turns and moves the lid member above the container installation portion.
  3. 　前記駆動手段は、前記蓋部材を旋回させることにより、前記容器の上方から該蓋部材を退避させる請求項２に記載の容器開閉装置。 3. The container opening and closing device according to claim 2, wherein the driving means retreats the lid member from above the container by turning the lid member.
  4. 　前記穴の中心の旋回軌道が、前記円周と異なっている、請求項２又は３に記載の容器開閉装置。 The container opening and closing device according to claim 2 or 3, wherein a turning trajectory at the center of the hole is different from the circumference.
PCT/JP2013/067263 2012-06-25 2013-06-24 Container opening/closing device WO2014002956A1 (en)

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2018150943A1 (en) * 2017-02-15 2018-08-23 コニカミノルタ株式会社 Liquid delivery system, inspection system, and liquid delivery method

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP3029592U (en) * 1995-03-29 1996-10-01 ジョンソン エンド ジョンソン クリニカル ダイアグノスティックス，インコーポレイティド Coupling device with bottle closure mechanism
JPH10129706A (en) * 1996-10-31 1998-05-19 Zojirushi Corp Drink container
JP2011185737A (en) * 2010-03-09 2011-09-22 Shimadzu Corp Autosampler

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS5815904Y2 (en) * 1979-09-28 1983-03-31 オムロン株式会社 Dispensing sample contamination prevention device
JPH01134255U (en) * 1988-03-07 1989-09-13
JP2006058212A (en) * 2004-08-23 2006-03-02 Nippon Pulse Motor Co Ltd Dispensing table equipped with heat-retaining function

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP3029592U (en) * 1995-03-29 1996-10-01 ジョンソン エンド ジョンソン クリニカル ダイアグノスティックス，インコーポレイティド Coupling device with bottle closure mechanism
JPH10129706A (en) * 1996-10-31 1998-05-19 Zojirushi Corp Drink container
JP2011185737A (en) * 2010-03-09 2011-09-22 Shimadzu Corp Autosampler

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2018150943A1 (en) * 2017-02-15 2018-08-23 コニカミノルタ株式会社 Liquid delivery system, inspection system, and liquid delivery method
JPWO2018150943A1 (en) * 2017-02-15 2019-12-12 コニカミノルタ株式会社 Liquid feeding system, inspection system and liquid feeding method

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