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WO2013115456A1 - Food, cosmetic material, or pharmaceutical composition containing ginger supercritical extract and fermented dropwort residue extract - Google Patents

Food, cosmetic material, or pharmaceutical composition containing ginger supercritical extract and fermented dropwort residue extract Download PDF

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Publication number
WO2013115456A1
WO2013115456A1 PCT/KR2012/008252 KR2012008252W WO2013115456A1 WO 2013115456 A1 WO2013115456 A1 WO 2013115456A1 KR 2012008252 W KR2012008252 W KR 2012008252W WO 2013115456 A1 WO2013115456 A1 WO 2013115456A1
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WIPO (PCT)
Prior art keywords
extract
composition
fermented
ginger
water
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PCT/KR2012/008252
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French (fr)
Korean (ko)
Inventor
양선아
이은주
정연섭
최희돈
김민주
임효권
이삼빈
이인선
김형대
Original Assignee
한국보건산업진흥원
계명대학교 산학협력단
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Publication of WO2013115456A1 publication Critical patent/WO2013115456A1/en

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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/23—Apiaceae or Umbelliferae (Carrot family), e.g. dill, chervil, coriander or cumin
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/88—Liliopsida (monocotyledons)
    • A61K36/906—Zingiberaceae (Ginger family)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/88—Liliopsida (monocotyledons)
    • A61K36/906—Zingiberaceae (Ginger family)
    • A61K36/9068—Zingiber, e.g. garden ginger
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/02—Cosmetics or similar toiletry preparations characterised by special physical form
    • A61K8/0216—Solid or semisolid forms
    • A61K8/022—Powders; Compacted Powders
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/02—Cosmetics or similar toiletry preparations characterised by special physical form
    • A61K8/0216—Solid or semisolid forms
    • A61K8/0229—Sticks
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/02—Cosmetics or similar toiletry preparations characterised by special physical form
    • A61K8/04—Dispersions; Emulsions
    • A61K8/06—Emulsions
    • A61K8/062—Oil-in-water emulsions
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/02—Cosmetics or similar toiletry preparations characterised by special physical form
    • A61K8/04—Dispersions; Emulsions
    • A61K8/06—Emulsions
    • A61K8/064—Water-in-oil emulsions, e.g. Water-in-silicone emulsions
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
    • A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
    • A61K8/9783—Angiosperms [Magnoliophyta]
    • A61K8/9789—Magnoliopsida [dicotyledons]
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
    • A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
    • A61K8/9783—Angiosperms [Magnoliophyta]
    • A61K8/9794—Liliopsida [monocotyledons]
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K9/00—Medicinal preparations characterised by special physical form
    • A61K9/06—Ointments; Bases therefor; Other semi-solid forms, e.g. creams, sticks, gels
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K9/00—Medicinal preparations characterised by special physical form
    • A61K9/08—Solutions
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K9/00—Medicinal preparations characterised by special physical form
    • A61K9/10—Dispersions; Emulsions
    • A61K9/12—Aerosols; Foams
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K9/00—Medicinal preparations characterised by special physical form
    • A61K9/48—Preparations in capsules, e.g. of gelatin, of chocolate
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P17/00—Drugs for dermatological disorders
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q1/00—Make-up preparations; Body powders; Preparations for removing make-up
    • A61Q1/02—Preparations containing skin colorants, e.g. pigments
    • A61Q1/04—Preparations containing skin colorants, e.g. pigments for lips
    • A61Q1/06—Lipsticks
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q1/00—Make-up preparations; Body powders; Preparations for removing make-up
    • A61Q1/02—Preparations containing skin colorants, e.g. pigments
    • A61Q1/10—Preparations containing skin colorants, e.g. pigments for eyes, e.g. eyeliner, mascara
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q19/00—Preparations for care of the skin
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
    • A61K2236/30—Extraction of the material
    • A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
    • A61K2236/331—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using water, e.g. cold water, infusion, tea, steam distillation or decoction
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
    • A61K2236/30—Extraction of the material
    • A61K2236/37—Extraction at elevated pressure or temperature, e.g. pressurized solvent extraction [PSE], supercritical carbon dioxide extraction or subcritical water extraction

Definitions

  • the present invention relates to a food composition, a cosmetic composition, and a pharmaceutical composition containing a ginger supercritical extract and a fermented parsley extract as an active ingredient.
  • Inflammatory skin diseases are diseases in which a series of clinical signs and symptoms, such as itching, swelling, erythema, peeling, etc., are caused by various stimulating factors causing a series of inflammatory reactions in the skin epithelium.
  • Inflammatory skin diseases include atopic dermatitis, contact dermatitis, seborrhoic dermatitis and acne.
  • Atopic dermatitis is generally used in the same sense as eczema, which is an eczema-like skin lesion that occurs in people with atopic dermatitis.
  • the cause of atopic dermatitis has not been identified to date and is known to have a genetic tendency, and now it is persuaded that it is a kind of autoimmune disease. Unlike normal eczema or dermatitis, it shows unusual symptoms and course, and 70 to 80% of pediatric eczema is present, and it occurs in adults recently.
  • Contact dermatitis is an inflammation of the skin caused by contact with an external substance. It has the same symptoms as acute eczema, but differs from eczema in that it occurs as a response to certain externally acting substances.
  • Seborrheic dermatitis is a dermatitis that occurs well in areas with high secretion of sebum, such as the head, forehead, and armpits. It is also called seborrheic eczema. A lot of erythema and slender slits (dandruff) occur and appear in many 20s and 40s. Unlike ordinary eczema, the disease is caused by constitution or sebaceous abnormalities, sensitive to sunlight or heat, often worsening in spring and autumn, and easy to recur.
  • Acne is a chronic inflammatory disease of hair follicles and sebaceous glands, the main cause of the increase in sebum secretion, anaerobic skin flora propionibacterium acnes (Propionibacterium acnes) is the main cause.
  • Sebum in the acne-prone areas is converted to testosterone (textosterone), a type of male hormone, into dihydrotestosterone, an active form, by 5 alpha-reductase. Is produced by oversecretion.
  • the excess sebum produced above stagnates in the hair follicles and is converted into free fatty acids and various low molecular substances by lipolytic enzymes and chemotactic factors secreted by propionibacterium acne, an anaerobic bacterium that sebum occludes the hair follicles. do. This causes white blood cells to gather around the hair follicles, destroying the hair follicle wall, causing the contents of the hair follicles to leak into the dermis, causing an inflammatory reaction.
  • Acne has been used to treat acne by using an antibiotic such as erythromycin or a method of controlling sebum using estrogen, a female hormone, but there are problems associated with side effects.
  • Vitamin A derivatives, benzoyl peroxide, salicylic acid, triclosan and the like have been used in the cosmetics for the treatment of acne, but these substances have some antibacterial effects, but have problems such as skin redness, skin sensitization, or photosensitivity.
  • the inventors of the present invention are developing a composition having no side effects that can effectively prevent or treat inflammatory skin diseases.
  • Ginger supercritical extract and fermented parsley extract are remarkably excellent in preventing or treating inflammatory skin diseases compared to a single substance.
  • the present invention was completed by confirming that it can be safely used in a living body without showing toxicity.
  • the present invention is to provide a food composition, cosmetic composition or pharmaceutical composition for the prevention or improvement of inflammatory skin diseases such as atopic dermatitis, acne, contact dermatitis by containing ginger supercritical extract and fermented parsley extract as an active ingredient.
  • the present invention provides a composition for the prevention, improvement or treatment of inflammatory skin diseases containing ginger supercritical extract and fermented parsley extract as an active ingredient.
  • the present invention provides a food, cosmetic or pharmaceutical composition containing a ginger supercritical extract and fermented parsley extract as an active ingredient.
  • the content of the active ingredient may be in the range of 0.05% to 95% by weight.
  • the fermented buttercup extract may be contained in 50 to 2000 parts by weight relative to 100 parts by weight of ginger supercritical extract.
  • the ginger supercritical extract is a ginger powder for 1 to 3 hours at a temperature of 35 ⁇ 55 °C, 1 ⁇ 3mL / min at a pressure of 100 ⁇ 400 bar using supercritical carbon dioxide Extracted by supercritical extraction.
  • the fermented buttercup gourd extract is mixed with 40 to 90% by weight of parsley and 10 to 60% by weight of sugar, and fermented at 15 to 30 °C for 8 to 24 months, after filtering the fermentation broth remaining parsley It can be obtained by extracting the hot water.
  • the ginger powder may have an average particle size of 50 ⁇ 500 ⁇ m.
  • the sugar may be one selected from the group consisting of brown sugar, white sugar, syrup and honey.
  • the formulation of the cosmetic composition is a basic cosmetic formulation consisting of a lotion, gel, water-soluble liquid, cream, essence, oil-in-water (O / W) type and water-in-oil (W / O) type;
  • Color cosmetic formulations consisting of oil-in-water or water-in-oil makeup bases, foundations, skin covers, lipsticks, lip gloss, face powders, two-way cakes, eye shadows, cheek colors and eyebrow pencils; It may be any one selected from.
  • Cosmetic composition according to the embodiment may be for skin irritation relief.
  • Cosmetic composition according to the embodiment may be for atopic skin improvement.
  • Cosmetic composition according to the embodiment may be for acne prevention.
  • the present invention provides a pharmaceutical composition for the prevention or treatment of inflammatory skin diseases, comprising ginger supercritical extract and fermented parsley extract as an active ingredient.
  • Formulation of the pharmaceutical composition for the prevention or treatment of inflammatory skin disease according to the embodiment, PLASTERS, lotion (LPTIONS), linings (LINIMENTS), liquid (LIQUIDS AND SOLUTIONS), aerosol (AEROSOLS) , EXTRACTS, OINTMENTS, FLUIDEXTRACTS, Emulsions, Emulsions, SUSPENSIONS, CAPSULES, CREAMS, Soft or Hard Gelatin Campels, Patches, It may be any one selected from sustained release agent.
  • the present invention can provide a food composition, cosmetic composition or pharmaceutical composition excellent in the prevention and improvement effect on inflammatory skin diseases by containing the supercritical extract of ginger and fermented parsley extract in order to maximize the anti-inflammatory effect of ginger. .
  • Figure 1 is a graph showing the ⁇ -hexosaminidase release inhibitory ability of the ginger supercritical extract according to an embodiment of the present invention.
  • Figure 2 is a graph showing the cytotoxicity of the ginger supercritical extract according to a preferred experimental example of the present invention.
  • Figure 3 is a graph showing the ⁇ -hexosaminidase release inhibition ability of the fermented buttercup gourd extract according to an experimental example of the present invention.
  • Figure 4 is a graph showing the cytotoxicity of the fermented buttercup gourd extract according to an embodiment of the present invention.
  • FIG. 5 is a graph showing ⁇ -hexosaminidase release inhibitory ability (antiallergic) of a composition containing a ginger supercritical extract and a fermented parsley extract according to an embodiment of the present invention.
  • the present invention provides a food composition for the prevention and improvement of inflammatory skin diseases by containing ginger supercritical extract and fermented parsley extract as an active ingredient.
  • the food composition containing the ginger supercritical extract and fermented parsley extract of the present invention has not only a high anti-inflammatory effect but also no toxic effect, which is very suitable for the prevention and improvement of inflammatory skin diseases.
  • the food composition may be used in the form of tea, beverages, dietary supplements, capsules and the like.
  • the present invention also provides a cosmetic composition containing a ginger supercritical extract and fermented parsley extract as an active ingredient.
  • the content of the active ingredient is preferably in the range 0.05 ⁇ 95% by weight relative to the total weight of the composition.
  • the fermented buttercup gourd extract in the composition is preferably contained at 50 to 2000 parts by weight relative to 100 parts by weight of the ginger supercritical extract because the anti-inflammatory effect is high.
  • the ginger supercritical extract is obtained by extracting ginger powder by supercritical extraction for 1 to 3 hours at a flow rate of 1 to 3 mL / min at a temperature of 35 to 55 ° C. and a pressure of 100 to 400 bar using supercritical carbon dioxide.
  • the ginger powder has an average particle size of 50 to 500 ⁇ m, and thus the extraction speed is high.
  • a ginger supercritical extract having a content of 21 to 25% by weight of gingerol, which is one of the anti-inflammatory active ingredients, may be obtained.
  • the fermented buttercup gourd extract is mixed with 40 to 90% by weight of parsley and 10 to 60% by weight of sugar, fermented at 15 to 30 ° C for 8 to 24 months, and then filtered from the fermentation broth to extract the remaining buttercup (residue by-product). It can be obtained by. After hydrothermal extraction of the fermented buttercup gourd may be further subjected to concentration and lyophilization as necessary. According to the above method, the fermented buttercup gourd extract can be obtained with a high yield of 15-25% on average.
  • the sugar may be one or more selected from the group consisting of brown sugar, white sugar, starch syrup and honey.
  • the ginger supercritical extract and the fermented parsley extract each have anti-inflammatory effects, but when the two substances are mixed, they may exhibit increased anti-inflammatory effects.
  • the cosmetic composition containing the ginger supercritical extract and fermented parsley extract of the present invention as an active ingredient may be used for skin irritation relief, atopic skin improvement or acne prevention.
  • Formulation of the cosmetic composition is a cosmetic composition consisting of a lotion, gel, water-soluble liquid, cream, essence, oil-in-water (O / W) type and water-in-oil (W / O) type;
  • Color cosmetic formulations consisting of oil-in-water or water-in-oil makeup bases, foundations, skin covers, lipsticks, lip gloss, face powders, two-way cakes, eye shadows, cheek colors and eyebrow pencils; It may be any one selected from.
  • the present invention provides a pharmaceutical composition for the prevention and treatment of inflammatory skin diseases by containing a ginger supercritical extract and fermented parsley extract as an active ingredient.
  • the pharmaceutical composition containing the ginger supercritical extract and fermented parsley extract of the present invention has not only a high anti-inflammatory effect but also no toxic effect, which is very suitable for the prevention and treatment of inflammatory skin diseases.
  • Formulations of pharmaceutical compositions include PLASTERS, LOTIONS, LINIMENTS, LIQUIDS AND SOLUTIONS, AEROSOLS, EXTRACTS, OINTMENTS, Fluid X FLUIDEXTRACTS, EMULSIONS, SUSPENSIONS, CAPSULES, CREAMS, soft or hard gelatin campels, patches, sustained release agents.
  • the dosage of the supercritical extract of ginger and fermented parsley extract according to the present invention as an active ingredient may be appropriately selected according to the age, sex, condition, and symptoms of an inflammatory skin disease patient, and preferably 1 day 1 To 500 mg of ginger supercritical extract and fermented parsley extract may be smeared on the affected area.
  • a supercritical fluid extraction device (SFT-100XW, Supercritical Fluid Technologies, Inc., Newark, DE, USA) was used, and 18 g of ginger powder having an average particle size of 50 to 500 ⁇ m was extracted into an extraction tank having a volume of 100 mL.
  • the extraction conditions of the samples were carried out with varying pressure at 35, 45, 55 °C temperature to 100, 200, 300, 400 bar, the flow rate of carbon dioxide is 2 mL / min for 2 hours in the dynamic mode to extract the container immediately I received it in (vial).
  • composition of the present invention was prepared by mixing 100 ⁇ g / mL of the prepared ginger supercritical extract and 1000 ⁇ g / mL of the fermented buttercup extract.
  • RAW264.7 cell line (murine macrophage cell line) was obtained from Korea Cell Line Bank (KCLB, Seoul, Korea) and DMEM with 10% FBS (fetal bovine serum) and 1% antibiotics (penicillin / streptomycin) The medium was passaged 2-3 times a week in a 37 °C incubator containing 5% CO 2 .
  • Cytotoxicity against mast cells was measured by MTT method.
  • the cell line RBL-2H3 cells used in this experiment were tested by subcultured through the pre-cultured through the Korea Cell Line Bank (Seoul, Korea).
  • the medium used for cell culture was used after culturing for 2-3 days in 5% CO 2 incubator at 37 ° C using MEM medium containing 10% FBS (fetal bovine serum) and 1% antibiotics (penicillin / streptomycin). .
  • the mast cell line RBL-2H3 was sensitized in 1 ml (2 x 10 5 cells, 24 well plates) for 16 hours with anti-DNP IgE (0.45 ⁇ g / ml) and 30 minutes before activation with DNP-BSA (10 ⁇ g / mL). 20 ml of the sample for each concentration was treated at 37 °C. After the reaction, 25 ⁇ l of the supernatant obtained by centrifugation at 400 ⁇ g for 10 minutes at 4 ° C. was reacted with 25 ⁇ l of substrate p-nitrophenyl-N-acetyl-bD-glucosaminide at 37 ° C. for 1 hour, followed by stop solution (0.1 M NaCO / NaHCO, pH 10) 200 ⁇ l was added to stop and the absorbance was measured at 405 nm to calculate the amount of ⁇ -hexosaminidase.
  • the mixture of ginger supercritical extract and fermented parsley extract is more effective than each single substance, they are mixed in non-toxic concentration intervals to effect the effect on mast cell activation ( ⁇ -hexosaminidase release inhibitory activity).
  • the ginger supercritical extract showed 33% of inhibitory activity at 100 ⁇ g / mL (Fig. 1)
  • the fermented buttercup extract showed 76% of inhibitory activity at 1000 ⁇ g / mL ( Figure 3).
  • the inhibitory activity was 89%, confirming the synergistic effect of the inhibitory effect (FIG. 5).
  • composition containing the ginger supercritical extract and the fermented parsley extract of the present invention can be seen that the skin improvement effect is superior to the case of using only each single substance.
  • cosmetic compositions such as nourishing cream, mist and body wash containing the ginger supercritical extract obtained in Example 1 and the fermented parsley extract were prepared according to a conventional method.
  • a cream containing a mixture of the ginger supercritical extract and fermented buttercup extract according to the present invention prepared in Preparation Example 1 on the affected part of acne patients (7) and atopic dermatitis patients (7) in an appropriate amount 2 After applying for one week in a manner that is applied twice, the state change of the affected part was observed.
  • the present invention can provide a food composition, cosmetic composition or pharmaceutical composition excellent in the prevention and improvement effect on inflammatory skin diseases by containing the supercritical extract of ginger and fermented parsley extract in order to maximize the anti-inflammatory effect of ginger. .

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Abstract

The present invention relates to a cosmetic material or a pharmaceutical composition containing, as active components, a ginger supercritical extract and a fermented dropwort residue extract, and more specifically, to a cosmetic material composition or a pharmaceutical composition containing an extract obtained by a supercritical extraction of ginger and a fermented dropwort residue extract in order to maximize the anti-inflammatory effect of the ginger, thereby providing superior preventative and improvement effects for inflammatory skin diseases.

Description

생강 초임계 추출물 및 발효 미나리 박 추출물을 함유하는 식품, 화장료 또는 약학 조성물Food, cosmetic or pharmaceutical composition containing ginger supercritical extract and fermented parsley extract
본 발명은 생강 초임계 추출물 및 발효 미나리 박 추출물을 유효성분으로 함유하는 식품 조성물, 화장료 조성물 및 약학 조성물에 관한 것이다. The present invention relates to a food composition, a cosmetic composition, and a pharmaceutical composition containing a ginger supercritical extract and a fermented parsley extract as an active ingredient.
염증성 피부질환이란 피부 상피 내에 일련의 염증 반응을 일으키는 다양한 자극 요인들로 인해 가려움, 부종, 홍반, 벗겨짐 등과 같은 일련의 임상적 징후와 증상이 유발되는 질환을 말한다.Inflammatory skin diseases are diseases in which a series of clinical signs and symptoms, such as itching, swelling, erythema, peeling, etc., are caused by various stimulating factors causing a series of inflammatory reactions in the skin epithelium.
염증성 피부질환으로는 아토피성 피부염(atopic dermatitis), 접촉성 피부염(contact dermatitis), 지루성 피부염(seborrhoic dermatitis) 및 여드름 등이 알려져 있다. 아토피성 피부염은 일반적으로 습진(eczema)과 같은 의미로 쓰이는데 아토피 체질인 사람에게 생기는 습진 모양의 피부병변으로서 내인성 습진 또는 베니에 양진이라고도 한다. 아토피성 피부염의 원인은 현재까지 밝혀져 있지 않으며 유전적인 경향이 있는 것으로 알려져 있고 현재는 일종의 자가면역 질환이라는 설이 설득력을 얻고 있다. 보통의 습진이나 피부염과는 달리 특이한 증세와 경과를 나타내는데 소아습진의 70~80%가 이에 해당하며 최근 들어 성인에게서도 많이 발생한다.Inflammatory skin diseases include atopic dermatitis, contact dermatitis, seborrhoic dermatitis and acne. Atopic dermatitis is generally used in the same sense as eczema, which is an eczema-like skin lesion that occurs in people with atopic dermatitis. The cause of atopic dermatitis has not been identified to date and is known to have a genetic tendency, and now it is persuaded that it is a kind of autoimmune disease. Unlike normal eczema or dermatitis, it shows unusual symptoms and course, and 70 to 80% of pediatric eczema is present, and it occurs in adults recently.
접촉성 피부염은 외부의 물질에 접촉함으로써 일어나는 피부의 염증이다. 급성습진과 같은 증세를 띠지만, 외부에서 작용하는 특정의 물질에 대한 반응으로서 일어나는 점이 습진과는 다른 점이다.Contact dermatitis is an inflammation of the skin caused by contact with an external substance. It has the same symptoms as acute eczema, but differs from eczema in that it occurs as a response to certain externally acting substances.
지루성 피부염은 머리, 이마, 겨드랑이 등 피지의 분비가 많은 부위에 잘 발생하는 피부염이며 지루성 습진이라고도 한다. 홍반과 가느다란 인설(비듬)이 많이 생기며 20~40대에서 많이 나타난다. 보통의 습진과 달리, 체질이나 피지분비 이상에 의하여 발생되는 질환으로서 햇빛이나 온열에 민감하고, 봄과 가을에 악화하는 일이 많으며, 재발하기 쉬운 특징이 있다.Seborrheic dermatitis is a dermatitis that occurs well in areas with high secretion of sebum, such as the head, forehead, and armpits. It is also called seborrheic eczema. A lot of erythema and slender slits (dandruff) occur and appear in many 20s and 40s. Unlike ordinary eczema, the disease is caused by constitution or sebaceous abnormalities, sensitive to sunlight or heat, often worsening in spring and autumn, and easy to recur.
여드름은 모낭 및 피지선에 발생하는 만성 염증성 질환으로서 발생원인은 크게 피지분비의 증가, 혐기성 피부 상재균인 프로피오니박테리움 아크네(Propionibacterium acnes)의 증식이 주원인이라 할 수 있다. 또한 여러 가지 기전이 복합적으로 작용하기도 한다. 여드름이 많이 발생하는 부위의 피지는 남성 호르몬의 일종인 테스토스테론(textosterone)이 5알파-환원효소(5a-reductase)에 의하여 활성형태인 디하이드로테스토스테론(dihydrotestosterone)으로 전환되고, 이 호르몬의 작용으로 피지가 과잉 분비되어 생성된다. 상기에서 생성된 과잉의 피지는 모낭 내에 정체하게 되고 상기 피지가 모낭을 막아 상주하는 혐기성 세균인 프로피오니박테리움 아크네에서 분비되는 지방분해효소와 화학주성인자에 의해 유리지방산 및 각종 저분자 물질들로 전환된다. 이렇게 되면 백혈구가 모낭 주위에 모이고 이들이 모낭벽을 파괴하면 모낭 내용물이 진피 내로 유출되어 염증 반응이 일어나게 된다.Acne is a chronic inflammatory disease of hair follicles and sebaceous glands, the main cause of the increase in sebum secretion, anaerobic skin flora propionibacterium acnes (Propionibacterium acnes) is the main cause. Several mechanisms may also work in combination. Sebum in the acne-prone areas is converted to testosterone (textosterone), a type of male hormone, into dihydrotestosterone, an active form, by 5 alpha-reductase. Is produced by oversecretion. The excess sebum produced above stagnates in the hair follicles and is converted into free fatty acids and various low molecular substances by lipolytic enzymes and chemotactic factors secreted by propionibacterium acne, an anaerobic bacterium that sebum occludes the hair follicles. do. This causes white blood cells to gather around the hair follicles, destroying the hair follicle wall, causing the contents of the hair follicles to leak into the dermis, causing an inflammatory reaction.
현재까지는 상기와 같은 염증성 피부질환의 치료를 위해서 주로 항히스타민제, 비타민 연고, 부신피질호르몬제가 사용되어 왔다. 그러나 이러한 약물은 그 효과가 일시적인 경우가 대부분이고 부작용이 심한 경우도 많다.Until now, antihistamines, vitamin ointments, and corticosteroids have been mainly used for the treatment of inflammatory skin diseases. However, these drugs are often temporary and often have severe side effects.
특히, 아토피성 피부염의 경우에는 여러 종류의 5-리포키시게나제 저해제가 항 알레르기제의 후보 화합물로서 제안되어 왔고, 크로몰린이 알레르겐과 조직 마스트 세포와의 반응을 무효화시키는 작용을 함으로써 증상을 완화하는 것으로 알려져 있으나 상기 물질들도 임상적으로 효과가 뚜렷하지 않는 단점이 있다.In particular, in the case of atopic dermatitis, various 5-lipokishinase inhibitors have been proposed as candidate compounds for antiallergic agents, and chromoline alleviates the reaction of allergens with tissue mast cells. It is known that the above substances also have the disadvantage that the clinical effect is not obvious.
여드름의 치료에는 에리스로마이신 등의 항생제를 이용하거나 여성호르몬인 에스트로젠을 이용한 피지를 조절하는 방법 등이 사용되어 왔으나 부작용이 동반되는 문제점이 있다. 여드름 치료를 위한 화장료에 비타민 A 유도체나 벤조일 퍼옥사이드, 살리실산, 트리클로산 등이 사용되어 왔지만, 이러한 물질들은 어느 정도의 항균 효과는 있으나 피부발적, 피부과민 또는 광과민 등의 부작용을 일으키는 문제점을 지니고 있다.Acne has been used to treat acne by using an antibiotic such as erythromycin or a method of controlling sebum using estrogen, a female hormone, but there are problems associated with side effects. Vitamin A derivatives, benzoyl peroxide, salicylic acid, triclosan and the like have been used in the cosmetics for the treatment of acne, but these substances have some antibacterial effects, but have problems such as skin redness, skin sensitization, or photosensitivity.
이에 본 발명자들은 염증성 피부질환을 효과적으로 예방하거나 치료할 수 있는 부작용이 없는 조성물을 개발하던 중 생강 초임계 추출물과 발효 미나리 박 추출물이 단일 물질에 비해 염증성 피부질환을 예방 또는 치료하는 효과까지 현저하게 우수하며 독성을 나타내지 않아 생체에 안전하게 사용될 수 있음을 확인함으로써 본 발명을 완성하였다.Therefore, the inventors of the present invention are developing a composition having no side effects that can effectively prevent or treat inflammatory skin diseases. Ginger supercritical extract and fermented parsley extract are remarkably excellent in preventing or treating inflammatory skin diseases compared to a single substance. The present invention was completed by confirming that it can be safely used in a living body without showing toxicity.
본 발명은 생강 초임계 추출물과 발효 미나리 박 추출물을 유효성분으로 함유함으로써 아토피성 피부염, 여드름, 접촉성 피부염과 같은 염증성 피부질환의 예방 또는 개선용 식품 조성물, 화장료 조성물 또는 약학 조성물을 제공하고자 한다.The present invention is to provide a food composition, cosmetic composition or pharmaceutical composition for the prevention or improvement of inflammatory skin diseases such as atopic dermatitis, acne, contact dermatitis by containing ginger supercritical extract and fermented parsley extract as an active ingredient.
이에 본 발명은 바람직한 구현예로서, 생강 초임계 추출물 및 발효 미나리 박 추출물을 유효성분으로 함유하는 염증성 피부질환의 예방, 개선 또는 치료용 조성물을 제공한다.Accordingly, the present invention provides a composition for the prevention, improvement or treatment of inflammatory skin diseases containing ginger supercritical extract and fermented parsley extract as an active ingredient.
이에 본 발명은 바람직한 구현예로서, 생강 초임계 추출물 및 발효 미나리 박 추출물을 유효성분으로 함유하는 식품, 화장료 또는 약학 조성물을 제공한다.Accordingly, the present invention provides a food, cosmetic or pharmaceutical composition containing a ginger supercritical extract and fermented parsley extract as an active ingredient.
상기 구현예에 의한 조성물에서, 상기 유효성분의 함량은 0.05중량% 내지 95중량% 범위 내일 수 있다.In the composition according to the embodiment, the content of the active ingredient may be in the range of 0.05% to 95% by weight.
상기 구현예에 의한 조성물에서, 발효 미나리 박 추출물은 생강 초임계 추출물 100 중량부 대비 50 ~ 2000 중량부로 함유될 수 있다.In the composition according to the embodiment, the fermented buttercup extract may be contained in 50 to 2000 parts by weight relative to 100 parts by weight of ginger supercritical extract.
상기 구현예에 의한 조성물에서, 상기 생강 초임계 추출물은 생강 분말을 초임계 이산화탄소를 이용하여 35~55℃의 온도, 100~400 bar의 압력에서 1~3mL/min의 유속으로 1~3 시간 동안 초임계 추출법으로 추출하여 얻을 수 있다.In the composition according to the embodiment, the ginger supercritical extract is a ginger powder for 1 to 3 hours at a temperature of 35 ~ 55 ℃, 1 ~ 3mL / min at a pressure of 100 ~ 400 bar using supercritical carbon dioxide Extracted by supercritical extraction.
상기 구현예에 의한 조성물에서, 상기 발효 미나리 박 추출물은 미나리 40~90중량% 및 당류 10~60중량%을 혼합하고, 15~30℃에서 8~24개월간 발효시킨 후, 발효액을 걸러내고 남은 미나리박을 열수추출함으로써 얻을 수 있다.In the composition according to the embodiment, the fermented buttercup gourd extract is mixed with 40 to 90% by weight of parsley and 10 to 60% by weight of sugar, and fermented at 15 to 30 ℃ for 8 to 24 months, after filtering the fermentation broth remaining parsley It can be obtained by extracting the hot water.
상기 구현예에 의한 조성물에서, 생강 분말은 평균 입도가 50~500㎛일 수 있다.In the composition according to the embodiment, the ginger powder may have an average particle size of 50 ~ 500㎛.
상기 구현예에 의한 조성물에서, 상기 당류는 흑설탕, 백설탕, 물엿 및 꿀로 이루어진 군에서 선택된 한 가지일 수 있다.In the composition according to the embodiment, the sugar may be one selected from the group consisting of brown sugar, white sugar, syrup and honey.
상기 구현예에 의한 조성물에서, 상기 화장료 조성물의 제형은 화장수, 젤, 수용성 리퀴드, 크림, 에센스, 수중유(O/W)형 및 유중수(W/O)형으로 이루어진 기초화장료 제형; 수중유형 또는 유중수형의 메이크업베이스, 파운데이션, 스킨커버, 립스틱, 립그로스, 페이스파우더, 투웨이케익, 아이섀도, 치크칼라 및 아이브로우펜슬류로 이루어진 색조화장료 제형; 중에서 선택되는 어느 하나일 수 있다.In the composition according to the embodiment, the formulation of the cosmetic composition is a basic cosmetic formulation consisting of a lotion, gel, water-soluble liquid, cream, essence, oil-in-water (O / W) type and water-in-oil (W / O) type; Color cosmetic formulations consisting of oil-in-water or water-in-oil makeup bases, foundations, skin covers, lipsticks, lip gloss, face powders, two-way cakes, eye shadows, cheek colors and eyebrow pencils; It may be any one selected from.
상기 구현예에 의한 화장료 조성물은 피부자극 완화용일 수 있다. Cosmetic composition according to the embodiment may be for skin irritation relief.
상기 구현예에 의한 화장료 조성물은 아토피 피부 개선용일 수 있다.Cosmetic composition according to the embodiment may be for atopic skin improvement.
상기 구현예에 의한 화장료 조성물은 여드름 예방용일 수 있다.Cosmetic composition according to the embodiment may be for acne prevention.
본 발명은 바람직한 구현예로서, 생강 초임계 추출물 및 발효 미나리 박 추출물을 유효성분으로 함유하는 염증성 피부질환의 예방 또는 치료용 약학 조성물을 제공한다.As a preferred embodiment, the present invention provides a pharmaceutical composition for the prevention or treatment of inflammatory skin diseases, comprising ginger supercritical extract and fermented parsley extract as an active ingredient.
상기 구현예에 의한 염증성 피부질환의 예방 또는 치료용 약학 조성물의 제형은, 경고제(PLASTERS), 로션제(LPTIONS), 리니멘트제(LINIMENTS), 액제(LIQUIDS AND SOLUTIONS), 에어로솔제(AEROSOLS), 엑스제(EXTRACTS), 연고제(OINTMENTS), 유동엑스제(FLUIDEXTRACTS), 유제(EMULSIONS), 현탁제(SUSPENSIONS), 캅셀제(CAPSULES), 크림제(CREAMS), 연질 또는 경질 젤라틴 캄셀, 첩부제, 서방화제제 중 선택된 어느 하나일 수 있다.Formulation of the pharmaceutical composition for the prevention or treatment of inflammatory skin disease according to the embodiment, PLASTERS, lotion (LPTIONS), linings (LINIMENTS), liquid (LIQUIDS AND SOLUTIONS), aerosol (AEROSOLS) , EXTRACTS, OINTMENTS, FLUIDEXTRACTS, Emulsions, Emulsions, SUSPENSIONS, CAPSULES, CREAMS, Soft or Hard Gelatin Campels, Patches, It may be any one selected from sustained release agent.
본 발명은 생강이 지닌 항염 효능을 극대화하기 위하여 생강을 초임계 추출한 추출물과 발효 미나리 박 추출물을 함유함으로써 염증성 피부질환에 대하여 예방 및 개선 효과가 우수한 식품 조성물, 화장료 조성물 또는 약학 조성물을 제공할 수 있다.The present invention can provide a food composition, cosmetic composition or pharmaceutical composition excellent in the prevention and improvement effect on inflammatory skin diseases by containing the supercritical extract of ginger and fermented parsley extract in order to maximize the anti-inflammatory effect of ginger. .
도 1은 본 발명의 바람직한 일 실험예에 따른 생강 초임계 추출물의 β-hexosaminidase 방출 억제능을 나타내는 그래프이다.Figure 1 is a graph showing the β-hexosaminidase release inhibitory ability of the ginger supercritical extract according to an embodiment of the present invention.
도 2는 본 발명의 바람직한 일 실험예에 따른 생강 초임계 추출물의 세포독성을 나타내는 그래프이다.Figure 2 is a graph showing the cytotoxicity of the ginger supercritical extract according to a preferred experimental example of the present invention.
도 3은 본 발명의 바람직한 일 실험예에 따른 발효 미나리 박 추출물의 β-hexosaminidase 방출 억제능을 나타내는 그래프이다.Figure 3 is a graph showing the β-hexosaminidase release inhibition ability of the fermented buttercup gourd extract according to an experimental example of the present invention.
도 4는 본 발명의 바람직한 일 실험예에 따른 발효 미나리 박 추출물의 세포독성을 나타내는 그래프이다.Figure 4 is a graph showing the cytotoxicity of the fermented buttercup gourd extract according to an embodiment of the present invention.
도 5는 본 발명의 바람직한 일 실시예에 따른 생강 초임계 추출물과 발효 미나리 박 추출물을 함유하는 조성물의 β-hexosaminidase 방출 억제능(항알레르기성)을 나타내는 그래프이다.FIG. 5 is a graph showing β-hexosaminidase release inhibitory ability (antiallergic) of a composition containing a ginger supercritical extract and a fermented parsley extract according to an embodiment of the present invention.
이하, 본 발명을 더욱 상세히 설명한다.Hereinafter, the present invention will be described in more detail.
우선, 본 발명은 생강 초임계 추출물 및 발효 미나리 박 추출물을 유효성분으로 함유함으로써 염증성 피부질환의 예방 및 개선용 식품 조성물을 제공한다. 본 발명의 생강 초임계 추출물과 발효 미나리 박 추출물을 함유하는 식품 조성물은 높은 항염 효과를 지니고 있을 뿐 아니라 독성 효과가 없으므로 염증성 피부질환의 예방 및 개선용으로 매우 적합하다. 상기한 식품 조성물은 차, 음료, 건강 보조식품, 캡슐 등의 형태로 이용될 수 있다.First, the present invention provides a food composition for the prevention and improvement of inflammatory skin diseases by containing ginger supercritical extract and fermented parsley extract as an active ingredient. The food composition containing the ginger supercritical extract and fermented parsley extract of the present invention has not only a high anti-inflammatory effect but also no toxic effect, which is very suitable for the prevention and improvement of inflammatory skin diseases. The food composition may be used in the form of tea, beverages, dietary supplements, capsules and the like.
또한, 본 발명은 생강 초임계 추출물 및 발효 미나리 박 추출물을 유효성분으로 함유하는 화장료 조성물을 제공한다. 이때, 유효성분의 함량은 조성물 총 중량 대비 0.05~95중량% 범위 내인 것이 바람직하다. 그리고, 상기 조성물 중 발효 미나리 박 추출물은 생강 초임계 추출물의 100 중량부 대비 50 ~ 2000 중량부로 함유되는 것이 항염 효과가 높으므로 바람직하다.The present invention also provides a cosmetic composition containing a ginger supercritical extract and fermented parsley extract as an active ingredient. At this time, the content of the active ingredient is preferably in the range 0.05 ~ 95% by weight relative to the total weight of the composition. In addition, the fermented buttercup gourd extract in the composition is preferably contained at 50 to 2000 parts by weight relative to 100 parts by weight of the ginger supercritical extract because the anti-inflammatory effect is high.
상기 생강 초임계 추출물은 생강 분말을 초임계 이산화탄소를 이용하여 35~55℃의 온도, 100~400 bar의 압력에서 1~3mL/min의 유속으로 1~3 시간 동안 초임계 추출법으로 추출하여 얻는 것이, 생강 특유의 맛과 향기를 제거하거나 보완할 수 있을 뿐 아니라 짧은 시간 안에 추출 수율이 높으면서도 진저롤(gingerol)의 함량이 높은 추출물을 얻을 수 있으므로 바람직하다. 상기 각각의 조건들이 범위를 벗어날 경우, 추출 수율이 낮아지거나 추출 수율이 더 이상 증가하지 않아 경제적으로 바람직하지 못하다.The ginger supercritical extract is obtained by extracting ginger powder by supercritical extraction for 1 to 3 hours at a flow rate of 1 to 3 mL / min at a temperature of 35 to 55 ° C. and a pressure of 100 to 400 bar using supercritical carbon dioxide. In addition to removing or supplementing the flavor and flavor peculiar to ginger, it is preferable to obtain an extract with a high extraction yield and a high content of gingerol in a short time. If the respective conditions are out of range, the extraction yield is lowered or the extraction yield is no longer increased, which is economically undesirable.
그리고, 상기 생강 초임계 추출물 제조방법에서 생강 분말은 평균입도가50~500㎛ 인 것이 추출 속도가 높으므로 바람직하다. In the ginger supercritical extract manufacturing method, the ginger powder has an average particle size of 50 to 500 μm, and thus the extraction speed is high.
상기 방법으로 생강을 초임계 추출하면 짧은 시간 안에 2.5~3.0중량%의 수율로 생강유(생강 초임계 추출물)를 얻을 수 있다.Supercritical extraction of ginger by the above method can obtain ginger oil (ginger supercritical extract) in a yield of 2.5 to 3.0% by weight in a short time.
그리고, 상기 추출방법에 의하면 항염 활성성분 중 하나인 진저롤(gingerol)의 함량이 21~25중량%인 생강 초임계 추출물을 얻을 수 있다. In addition, according to the extraction method, a ginger supercritical extract having a content of 21 to 25% by weight of gingerol, which is one of the anti-inflammatory active ingredients, may be obtained.
상기 발효 미나리 박 추출물은 미나리 40~90중량% 및 당류 10~60중량%을 혼합하고, 15~30℃에서 8~24개월간 발효시킨 후, 발효액을 걸러내고 남은 미나리박(잔여 부산물)을 열수추출함으로써 얻을 수 있다. 발효 미나리 박을 열수추출한 후에 필요에 따라 농축 및 동결건조 과정을 더 거칠 수 있다. 상기한 방법에 따르면 평균 15~25%의 높은 수율로 발효 미나리 박 추출물을 얻을 수 있다.The fermented buttercup gourd extract is mixed with 40 to 90% by weight of parsley and 10 to 60% by weight of sugar, fermented at 15 to 30 ° C for 8 to 24 months, and then filtered from the fermentation broth to extract the remaining buttercup (residue by-product). It can be obtained by. After hydrothermal extraction of the fermented buttercup gourd may be further subjected to concentration and lyophilization as necessary. According to the above method, the fermented buttercup gourd extract can be obtained with a high yield of 15-25% on average.
상기 발효 미나리 박 추출물 제조과정에서, 당류는 흑설탕, 백설탕, 물엿 및 꿀로 이루어진 군에서 선택된 한 가지 이상일 수 있다.In the fermented buttercup gourd extract manufacturing process, the sugar may be one or more selected from the group consisting of brown sugar, white sugar, starch syrup and honey.
상기한 생강 초임계 추출물 및 발효 미나리 박 추출물은 각각 항염 효능을 지니고 있지만 상기 두 물질이 혼합된 경우 더욱 증대된 항염 효능을 나타낼 수 있다.The ginger supercritical extract and the fermented parsley extract each have anti-inflammatory effects, but when the two substances are mixed, they may exhibit increased anti-inflammatory effects.
따라서, 본 발명의 생강 초임계 추출물 및 발효 미나리 박 추출물을 유효성분으로 함유하는 화장료 조성물은 피부자극 완화용, 아토피 피부 개선용 또는 여드름 예방용으로 이용될 수 있다. 상기 화장료 조성물의 제형은 화장수, 젤, 수용성 리퀴드, 크림, 에센스, 수중유(O/W)형 및 유중수(W/O)형으로 이루어진 기초화장료 제형; 수중유형 또는 유중수형의 메이크업베이스, 파운데이션, 스킨커버, 립스틱, 립그로스, 페이스파우더, 투웨이케익, 아이섀도, 치크칼라 및 아이브로우펜슬류로 이루어진 색조화장료 제형; 중에서 선택되는 어느 하나일 수 있다.Therefore, the cosmetic composition containing the ginger supercritical extract and fermented parsley extract of the present invention as an active ingredient may be used for skin irritation relief, atopic skin improvement or acne prevention. Formulation of the cosmetic composition is a cosmetic composition consisting of a lotion, gel, water-soluble liquid, cream, essence, oil-in-water (O / W) type and water-in-oil (W / O) type; Color cosmetic formulations consisting of oil-in-water or water-in-oil makeup bases, foundations, skin covers, lipsticks, lip gloss, face powders, two-way cakes, eye shadows, cheek colors and eyebrow pencils; It may be any one selected from.
다음으로, 본 발명은 생강 초임계 추출물 및 발효 미나리 박 추출물을 유효성분으로 함유함으로써 염증성 피부질환의 예방 및 치료용 약학 조성물을 제공한다. 본 발명의 생강 초임계 추출물과 발효 미나리 박 추출물을 함유하는 약학 조성물은 높은 항염 효과를 지니고 있을 뿐 아니라 독성 효과가 없으므로 염증성 피부질환의 예방 및 치료용으로 매우 적합하다.Next, the present invention provides a pharmaceutical composition for the prevention and treatment of inflammatory skin diseases by containing a ginger supercritical extract and fermented parsley extract as an active ingredient. The pharmaceutical composition containing the ginger supercritical extract and fermented parsley extract of the present invention has not only a high anti-inflammatory effect but also no toxic effect, which is very suitable for the prevention and treatment of inflammatory skin diseases.
약학 조성물의 제형은 경고제(PLASTERS), 로션제(LPTIONS), 리니멘트제(LINIMENTS), 액제(LIQUIDS AND SOLUTIONS), 에어로솔제(AEROSOLS), 엑스제(EXTRACTS), 연고제(OINTMENTS), 유동엑스제(FLUIDEXTRACTS), 유제(EMULSIONS), 현탁제(SUSPENSIONS), 캅셀제(CAPSULES), 크림제(CREAMS), 연질 또는 경질 젤라틴 캄셀, 첩부제, 서방화제제 중 선택된 어느 하나일 수 있다.Formulations of pharmaceutical compositions include PLASTERS, LOTIONS, LINIMENTS, LIQUIDS AND SOLUTIONS, AEROSOLS, EXTRACTS, OINTMENTS, Fluid X FLUIDEXTRACTS, EMULSIONS, SUSPENSIONS, CAPSULES, CREAMS, soft or hard gelatin campels, patches, sustained release agents.
본 발명에 따른 생강 초임계 추출물 및 발효 미나리 박 추출물을 유효성분으로서 투여하는 투여량은 염증성 피부질환 환자의 연령, 성별, 상태, 질병의 증상에 따라 적절히 선택될 수 있으며, 바람직하게는 1일 1 내지 500mg의 생강 초임계 추출물 및 발효 미나리 박 추출물을 환부에 도말할 수 있다.The dosage of the supercritical extract of ginger and fermented parsley extract according to the present invention as an active ingredient may be appropriately selected according to the age, sex, condition, and symptoms of an inflammatory skin disease patient, and preferably 1 day 1 To 500 mg of ginger supercritical extract and fermented parsley extract may be smeared on the affected area.
이하, 본 발명의 구성을 실시예를 통하여 보다 상세히 설명하나, 본 발명의 범위가 하기 실시예로 한정되는 것은 아니다.Hereinafter, the configuration of the present invention in more detail through examples, but the scope of the present invention is not limited to the following examples.
[실시예]EXAMPLE
1. 생강 초임계 추출물의 제조 1. Preparation of Ginger Supercritical Extract
초임계유체추출장치(SFT-100XW, Supercritical Fluid Technologies, Inc., Newark, DE, USA)를 사용하였고, 평균입도가 50 ~ 500㎛ 인 생강 분말 18g을 100 mL의 용량의 추출조에 넣어 추출하였다. 시료의 추출조건은 35, 45, 55℃온도에서 100, 200, 300, 400 bar로 압력을 변화시키며 실시하였고, 이산화탄소의 유속은 2 mL/min로 다이나믹 모드에서 2시간동안 추출하여 추출물을 곧바로 용기(vial)에 받아내었다.A supercritical fluid extraction device (SFT-100XW, Supercritical Fluid Technologies, Inc., Newark, DE, USA) was used, and 18 g of ginger powder having an average particle size of 50 to 500 µm was extracted into an extraction tank having a volume of 100 mL. The extraction conditions of the samples were carried out with varying pressure at 35, 45, 55 ℃ temperature to 100, 200, 300, 400 bar, the flow rate of carbon dioxide is 2 mL / min for 2 hours in the dynamic mode to extract the container immediately I received it in (vial).
2. 발효 미나리 박 추출물의 제조 2. Preparation of Fermented Buttercup Extract
대구 비슬산 가창면 정대리에서 수확한 청정미나리를 수세한 후 세척 및 살균된 옹기에 넣고 흑설탕(brown sugar)을 1:1의 중량비로 혼합한 후, 상온(15~25℃)에서 1년간 방치, 자연발효가 일어나도록 하여 미나리 발효액을 제조하였다. 그리고, 발효 후 남은 잔여 부산물(미나리박) 1kg을 수세하고 8L의 물을 가하여 2시간 열수 추출, 농축, 동결 건조하여 192.8g(19.3%의 수율)의 발효 미나리 박 추출물을 제조하였다.Wash the clean parsley harvested from Jeongdae-ri, Gachang-myeon, Daegu, Bisulsan, wash and sterilize, and mix brown sugar in a weight ratio of 1: 1, and leave it at room temperature (15 ~ 25 ℃) for 1 year Was produced to prepare a buttercup fermentation broth. Then, 1kg of the remaining by-products (minaribak) remaining after fermentation were washed with water and 8L of water was added thereto for 2 hours, followed by hot water extraction, concentration, and lyophilization to prepare 192.8g (19.3% yield) of fermented buttercup meal.
3. 생강 초임계 추출물 및 발효 미나리 박 추출물 함유 조성물 제조 3. Preparation of Ginger Supercritical Extract and Fermented Buttercup Extract
상기 제조된 생강 초임계 추출물 100㎍/mL과 상기 제조된 발효 미나리 박 추출물 1000㎍/mL 을 혼합하여 본 발명의 조성물을 제조하였다.The composition of the present invention was prepared by mixing 100 μg / mL of the prepared ginger supercritical extract and 1000 μg / mL of the fermented buttercup extract.
[실험방법]Experimental Method
1. 세포주 배양 1. Cell line culture
대식세포 계열(murine macrophage cell line)인 RAW264.7 세포주는 한국 세포주 은행(KCLB, Seoul, Korea)으로부터 분양받았으며, 10% FBS(fetal bovine serum)와 1% antibiotics(penicillin/ streptomycin)를 첨가한 DMEM 배지를 이용하여 5% CO2가 존재하는 37℃ incubator에서 1주일에 2~3회 계대 배양하였다.RAW264.7 cell line (murine macrophage cell line) was obtained from Korea Cell Line Bank (KCLB, Seoul, Korea) and DMEM with 10% FBS (fetal bovine serum) and 1% antibiotics (penicillin / streptomycin) The medium was passaged 2-3 times a week in a 37 ℃ incubator containing 5% CO 2 .
Rat 유래의 비만세포(RBL-2H3)에 대한 세포독성은 MTT 법으로 측정하였다. 본 실험에 사용한 세포주 RBL-2H3 cell은 한국 세포주 은행(Seoul, Korea)을 통해서 분양 받아 계대배양하면서 실험하였다. 세포배양에 사용된 배지는 10% FBS(fetal bovine serum)와 1% antibiotics(penicillin/streptomycin)를 첨가한 MEM 배지를 이용하여 37℃에서 5% CO2 incubator에서 2~3일간 배양한 후 사용하였다.Cytotoxicity against mast cells (RBL-2H3) derived from rats was measured by MTT method. The cell line RBL-2H3 cells used in this experiment were tested by subcultured through the pre-cultured through the Korea Cell Line Bank (Seoul, Korea). The medium used for cell culture was used after culturing for 2-3 days in 5% CO 2 incubator at 37 ° C using MEM medium containing 10% FBS (fetal bovine serum) and 1% antibiotics (penicillin / streptomycin). .
2. β-Hexosaminidase 방출 억제능 측정 2. Measurement of β-Hexosaminidase Release Inhibition
비만세포주 RBL-2H3을 1ml(2 x 105 cells, 24 well plate)에 anti-DNP IgE(0.45㎍/ml)로 16시간 감작시키고, DNP-BSA(10㎍/mL)로 활성화시키기 전에 30분 동안 37℃에서 농도별 시료를 20ml 처리하였다. 반응이 끝난 후 4℃에서 10분간 400xg에서 원침하여 얻은 상등액 25㎕를 기질 p-nitrophenyl-N-acetyl-b-D-glucosaminide 25㎕와 37℃에서 1시간 반응시킨 후 stop solution(0.1 M NaCO/NaHCO, pH 10) 200㎕를 가하여 정지시키고 405 nm에서 흡광도를 측정하여 β-hexosaminidase의 양을 계산하였다.The mast cell line RBL-2H3 was sensitized in 1 ml (2 x 10 5 cells, 24 well plates) for 16 hours with anti-DNP IgE (0.45 µg / ml) and 30 minutes before activation with DNP-BSA (10 µg / mL). 20 ml of the sample for each concentration was treated at 37 ℃. After the reaction, 25 μl of the supernatant obtained by centrifugation at 400 × g for 10 minutes at 4 ° C. was reacted with 25 μl of substrate p-nitrophenyl-N-acetyl-bD-glucosaminide at 37 ° C. for 1 hour, followed by stop solution (0.1 M NaCO / NaHCO, pH 10) 200 μl was added to stop and the absorbance was measured at 405 nm to calculate the amount of β-hexosaminidase.
[실험예]Experimental Example
실험예 1: 생강 초임계 추출물(생강 오일)의 세포 독성 및 β-hexosaminidase 방출 억제능 측정Experimental Example 1 Measurement of Cytotoxicity and β-hexosaminidase Inhibitory Activity of Ginger Supercritical Extract (Ginger Oil)
RBL-2H3 세포에 대한 생강 초임계 추출물의 독성 및 항원에 의한 비만세포 탈과립에 대한 억제 효과(β-hexosaminidase 방출 억제능)를 테스트한 결과, 테스트한 농도 구간에서 세포 독성은 없었으며, 농도 의존적으로 비만 세포 탈과립을 억제하는 효과를 나타내었다(도 1 및 도 2).Testing of the toxicity of Ginger supercritical extract against RBL-2H3 cells and the inhibitory effect of antigen-induced mast cell degranulation (β-hexosaminidase release inhibitory ability) showed no cytotoxicity in the tested concentration range, and was concentration-dependent obesity. The effect of inhibiting cell degranulation was shown (FIGS. 1 and 2).
실험예 2: 발효 미나리 박 추출물의 세포 독성 및 β-hexosaminidase 방출 억제능 측정Experimental Example 2: Measurement of Cytotoxicity and β-hexosaminidase Release Inhibition of Fermented Buttercup Extract
발효 후 부산물인 미나리 박의 열수 추출물이 비만세포 탈과립(β-hexosaminidase 방출 억제능)을 유의적으로 억제하는 효과가 있음을 나타내었으며, 세포 독성은 나타나지 않았다(도 3 및 도 4)After fermentation, the hot water extract of the parsley parsley, a byproduct, showed a significant effect of inhibiting mast cell degranulation (β-hexosaminidase release inhibitory ability), and no cytotoxicity was observed (FIGS. 3 and 4).
실험예 3: 생강 초임계 추출물과 발효 미나리 박 추출물의 혼합물의 세포 독성 및 β-hexosaminidase 방출 억제능(항알레르기) 측정Experimental Example 3: Determination of Cytotoxicity and β-hexosaminidase Release Inhibition (Antiallergic) of a Mixture of Ginger Supercritical Extract and Fermented Buttercup Extract
생강 초임계 추출물과 발효 미나리 박 추출물의 혼합물이 각각의 단일 물질에 비해 더 효과가 있음을 알아보기 위해, 각각 독성이 없는 농도 구간에서 혼합하여 비만세포 활성화(β-hexosaminidase 방출 억제능)에 대한 효과를 측정한 결과, 생강 초임계 추출물은 100㎍/mL에서 33%의 억제활성을 나타내었고(도 1), 발효 미나리 박 추출물은 1000㎍/mL에서 76%의 억제활성을 나타내었으며(도 3), 이 두 물질을 혼합한 혼합액의 경우에는 89%의 억제활성을 나타내어 억제 효과의 시너지 효과를 확인할 수 있었다(도 5).To find out that the mixture of ginger supercritical extract and fermented parsley extract is more effective than each single substance, they are mixed in non-toxic concentration intervals to effect the effect on mast cell activation (β-hexosaminidase release inhibitory activity). As a result, the ginger supercritical extract showed 33% of inhibitory activity at 100µg / mL (Fig. 1), and the fermented buttercup extract showed 76% of inhibitory activity at 1000µg / mL (Figure 3). In the mixed solution of these two substances, the inhibitory activity was 89%, confirming the synergistic effect of the inhibitory effect (FIG. 5).
이상 살펴본 바와 같이 본 발명의 생강 초임계 추출물 및 발효 미나리 박 추출물을 함유하는 조성물은 각각의 단일 물질만을 사용하는 경우보다 피부 개선 효과가 우수하다는 것을 알 수 있다.As described above, the composition containing the ginger supercritical extract and the fermented parsley extract of the present invention can be seen that the skin improvement effect is superior to the case of using only each single substance.
[제조예][Production example]
하기와 같이, 실시예 1에서 수득한 생강 초임계 추출물과 발효 미나리 박 추출물을 함유한 영양 크림, 미스트 및 바디 워시 등의 화장료 조성물을 통상의 방법에 따라 제조하였다.As described below, cosmetic compositions such as nourishing cream, mist and body wash containing the ginger supercritical extract obtained in Example 1 and the fermented parsley extract were prepared according to a conventional method.
제조예 1: 크림Preparation Example 1 Cream
표 1
성분 함량(%) 비고
정제수 71.39 Solvent
Isoamyl Laurate 8.0 Skin conditioning agent
Butylene Glycol 5.0 Skin conditioning agent
Glycerin 5.0 Skin conditioning agent
Glyceryl Stearate 4.0 Surfactant-Emulsifying Agent
Cetearyl Alcohol 2.0 Emusion Stabilizer
Caprylyl glycol 0.3 Skin conditioning agent
Caprylhydroxamic acid 0.1 Chelating Agent
생강 초임계 추출물 0.06 생강 초임계 추출, 항알러지 효과
발효 미나리 박 추출물 1.8 미나리 당발효 추출, 항알러지 효과
혼합추출물(홍삼, 사철쑥꽃, 형개, 회향, 서양측백나무잎) 1.2 Skin conditioning agent
동백오일 1.0 Skin conditioning agent
향료 0.15 -
Table 1
ingredient content(%) Remarks
Purified water 71.39 Solvent
Isoamyl laurate 8.0 Skin conditioning agent
Butylene glycol 5.0 Skin conditioning agent
Glycerin 5.0 Skin conditioning agent
Glyceryl Stearate 4.0 Surfactant-Emulsifying Agent
Cetearyl alcohol 2.0 Emusion Stabilizer
Caprylyl glycol 0.3 Skin conditioning agent
Caprylhydroxamic acid 0.1 Chelating Agent
Ginger Supercritical Extract 0.06 Ginger supercritical extract, anti-allergic effect
Fermented Buttercup Extract 1.8 Buttercup sugar extract, anti-allergic effect
Mixed extracts (red ginseng, cedar wormwood, hedgehog, fennel, cypress leaves) 1.2 Skin conditioning agent
Camellia oil 1.0 Skin conditioning agent
Spices 0.15 -
제조예 2: 미스트Preparation Example 2: Mist
표 2
성분 함량(%) 비고
정제수 67.48 Solvent
Butylene Glycol 2.5 Skin conditioning agent
Glycerin 3.0 Skin conditioning agent
에탄올 16.0 Solvent
Cyclodextrin 0.1 Absorbent
Caprylyl glycol 0.4 Skin conditioning agent
Caprylhydroxamic acid 0.1 Chelating Agent
생강 초임계 추출물 0.02 생강 초임계 추출, 항알러지 효과
발효 미나리 박 추출물 1.5 미나리 당발효 추출, 항알러지 효과
혼합추출물(홍삼, 사철쑥꽃, 형개, 회향, 서양측백나무잎) 8.0 Skin conditioning agent
Tocopheryl Acetate 0.1 Skin conditioning agent, 비타민 E 유도체
Niacinamide 0.3 Skin conditioning agent, Vitamin B3
Copper Tripeptide-1 0.3 Skin conditioning agent
Arginine 0.2 Skin conditioning agent, 아미노산
TABLE 2
ingredient content(%) Remarks
Purified water 67.48 Solvent
Butylene glycol 2.5 Skin conditioning agent
Glycerin 3.0 Skin conditioning agent
ethanol 16.0 Solvent
Cyclodextrin 0.1 Absorbent
Caprylyl glycol 0.4 Skin conditioning agent
Caprylhydroxamic acid 0.1 Chelating Agent
Ginger Supercritical Extract 0.02 Ginger supercritical extract, anti-allergic effect
Fermented Buttercup Extract 1.5 Buttercup sugar extract, anti-allergic effect
Mixed extracts (red ginseng, cedar wormwood, hedgehog, fennel, cypress leaves) 8.0 Skin conditioning agent
Tocopheryl Acetate 0.1 Skin conditioning agent, vitamin E derivative
Niacinamide 0.3 Skin conditioning agent, Vitamin B3
Copper Tripeptide-1 0.3 Skin conditioning agent
Arginine 0.2 Skin conditioning agent, amino acids
제조예 3: 바디 워시Preparation Example 3: Body Wash
표 3
성분 함량(%) 비고
정제수 64.78 Solvent
Sodium Laureth Sulfate 15.0 Surfactant-Cleansing Agent
Lauryl Glucoside 6.0 Surfactant-Cleansing Agent
Cocamidopropyl Betaine 6.0 Surfactant-Cleansing Agent
Caprylyl glycol 0.4 Skin conditioning agent
Caprylhydroxamic acid 0.1 Chelating Agent
생강 초임계 추출물 0.02 생강 초임계 추출, 항알러지 효과
발효 미나리 박 추출물 1.5 미나리 당발효 추출, 항알러지 효과
혼합추출물(홍삼, 사철쑥꽃, 형개, 회향, 서양측백나무잎) 5.0 Skin conditioning agent
Laurdimonium HydroxypropylHydrolyzed Wheat Protein 0.5 Antistatic agent
향료 0.7 -
TABLE 3
ingredient content(%) Remarks
Purified water 64.78 Solvent
Sodium Laureth Sulfate 15.0 Surfactant-Cleansing Agent
Lauryl glucoside 6.0 Surfactant-Cleansing Agent
Cocamidopropyl Betaine 6.0 Surfactant-Cleansing Agent
Caprylyl glycol 0.4 Skin conditioning agent
Caprylhydroxamic acid 0.1 Chelating Agent
Ginger Supercritical Extract 0.02 Ginger supercritical extract, anti-allergic effect
Fermented Buttercup Extract 1.5 Buttercup sugar extract, anti-allergic effect
Mixed Extract (Red Ginseng, Evergreen Mugwort, Hyungae, Fennel, Western Cypress Leaf) 5.0 Skin conditioning agent
Laurdimonium HydroxypropylHydrolyzed Wheat Protein 0.5 Antistatic agent
Spices 0.7 -
실험예 4: 생강 초임계 추출물과 발효 미나리 박 추출물의 혼합물의 임상적 치료 효과Experimental Example 4: Clinical therapeutic effect of a mixture of ginger supercritical extract and fermented parsley extract
여드름 환자(7명) 및 아토피성 피부염 환자(7명)의 환부에 상기 제조예 1에서 제조한 본 발명에 따른 생강 초임계 추출물과 발효 미나리 박 추출물의 혼합물을 포함하는 크림을 적당량으로 1일 2회 도포하는 방식으로 1주일간 도포한 후 환부의 상태변화를 관찰하였다.A cream containing a mixture of the ginger supercritical extract and fermented buttercup extract according to the present invention prepared in Preparation Example 1 on the affected part of acne patients (7) and atopic dermatitis patients (7) in an appropriate amount 2 After applying for one week in a manner that is applied twice, the state change of the affected part was observed.
실험 결과, 하기 표 4와 같이 본 발명에 따른 혼합 추출물이 포함된 크림을 여드름 환자 또는 아토피성 피부염 환자의 환부에 도포한 경우 여드름 또는 아토피성 피부염이 현저히 호전된 것을 관찰할 수 있었다.As a result, when the cream containing the mixed extract according to the present invention is applied to the affected part of acne patients or atopic dermatitis patients as shown in Table 4, it was observed that acne or atopic dermatitis was significantly improved.
표 4
피부질환종류 환자의 반응(Response) 합계
xx x △ ○ ○○
여드름 2 3 2 7
아토피성 피부염 3 3 1 7
합계 14
Table 4
Types of Skin Diseases Patient response Sum
xx x △ ○ ○○
acne 2 3 2 7
Atopic dermatitis 3 3 One 7
Sum 14
xx : 악화(aggrevation)xx: aggrevation
x : 효과 없음(no change)x: no change
△ : 다소 효과적(slightly effective)△: slightly effective
○ : 효과 있음(moderately effective)○: moderately effective
○○ : 매우 효과적(significantly effective)○○: very effective (significantly effective)
이상에서 본 발명의 바람직한 실시예, 실험예 및 제조예에 대하여 도시하고 또한 설명하였으나, 본 발명은 상기한 실시예 또는 제조예에 한정되지 않고, 이하 청구범위에서 청구하는 본 발명의 요지를 벗어남이 없이 당해 발명의 분야에서 통상의 지식을 가진 자라면 누구든지 다양한 변형실시가 가능함은 물론이며, 그와 같은 변형은 청구범위의 기재 범위 내에 있게 된다.Although preferred embodiments, experimental examples, and preparations of the present invention have been shown and described above, the present invention is not limited to the above-described examples or preparations, but deviates from the gist of the present invention as claimed in the following claims. Without departing from the scope of the present invention, it will be apparent to those skilled in the art that various modifications can be made without departing from the scope of the claims.
본 발명은 생강이 지닌 항염 효능을 극대화하기 위하여 생강을 초임계 추출한 추출물과 발효 미나리 박 추출물을 함유함으로써 염증성 피부질환에 대하여 예방 및 개선 효과가 우수한 식품 조성물, 화장료 조성물 또는 약학 조성물을 제공할 수 있다.The present invention can provide a food composition, cosmetic composition or pharmaceutical composition excellent in the prevention and improvement effect on inflammatory skin diseases by containing the supercritical extract of ginger and fermented parsley extract in order to maximize the anti-inflammatory effect of ginger. .

Claims (13)

  1. 생강 초임계 추출물 및 발효 미나리 박 추출물을 유효성분으로 함유하는 염증성 피부질환의 예방, 개선 또는 치료용 조성물.A composition for the prevention, improvement or treatment of inflammatory skin diseases containing ginger supercritical extract and fermented parsley extract as an active ingredient.
  2. 제1항에 있어서,The method of claim 1,
    상기 유효성분의 함량은 0.05중량% 내지 95중량%인 것을 특징으로 하는 조성물.The content of the active ingredient is a composition, characterized in that 0.05% to 95% by weight.
  3. 제1항에 있어서,The method of claim 1,
    발효 미나리 박 추출물은 생강 초임계 추출물 100 중량부 대비 50 ~ 2000 중량부로 함유된 것을 특징으로 하는 조성물.Fermented buttercup gourd extract is a composition comprising 50 to 2000 parts by weight relative to 100 parts by weight of ginger supercritical extract.
  4. 제1항에 있어서,The method of claim 1,
    상기 생강 초임계 추출물은 생강 분말을 초임계 이산화탄소를 이용하여 35~55℃의 온도, 100~400 bar의 압력에서 1~3mL/min의 유속으로 1~3 시간 동안 초임계 추출법으로 추출하여 얻은 것을 특징으로 하는 조성물.The ginger supercritical extract was obtained by extracting ginger powder by supercritical extraction for 1 to 3 hours at a flow rate of 1 to 3 mL / min at a temperature of 35 to 55 ° C. and a pressure of 100 to 400 bar using supercritical carbon dioxide. Characterized in that the composition.
  5. 제4항에 있어서,The method of claim 4, wherein
    생강 분말은 평균 입도가 50~500㎛인 것을 특징으로 하는 조성물.Ginger powder is characterized in that the average particle size of 50 ~ 500㎛.
  6. 제1항에 있어서,The method of claim 1,
    상기 발효 미나리 박 추출물은 미나리 40~90중량% 및 당류 10~60중량%을 혼합하고, 15~30℃에서 8~24개월간 발효시킨 후, 발효액을 걸러내고 남은 미나리박을 열수추출함으로써 얻어지는 것을 특징으로 하는 조성물.The fermented buttercup gourd extract is obtained by mixing 40 to 90% by weight of parsley and 10 to 60% by weight of sugar, fermenting at 15 to 30 ° C. for 8 to 24 months, filtering the fermentation broth, and extracting the remaining parsley. Composition.
  7. 제6항에 있어서,The method of claim 6,
    상기 당류는 흑설탕, 백설탕, 물엿 및 꿀로 이루어진 군에서 선택된 한 가지 이상인 것을 특징으로 하는 조성물.The sugar is a composition characterized in that at least one selected from the group consisting of brown sugar, white sugar, starch syrup and honey.
  8. 제1항에 있어서,The method of claim 1,
    식품 조성물인 것을 특징으로 하는 조성물.A composition comprising a food composition.
  9. 제1항에 있어서,The method of claim 1,
    화장료 조성물인 것을 특징으로 하는 조성물.A composition comprising a cosmetic composition.
  10. 제9항에 있어서,The method of claim 9,
    상기 화장료 조성물은 피부자극 완화용, 아토피 피부 개선용, 또는 여드름 예방용인 것을 특징으로 하는 조성물.The cosmetic composition is a composition for reducing skin irritation, improving atopic skin, or preventing acne.
  11. 제9항에 있어서,The method of claim 9,
    상기 화장료 조성물의 제형은 화장수, 젤, 수용성 리퀴드, 크림, 에센스, 수중유(O/W)형 및 유중수(W/O)형으로 이루어진 기초화장료 제형; 수중유형 또는 유중수형의 메이크업베이스, 파운데이션, 스킨커버, 립스틱, 립그로스, 페이스파우더, 투웨이케익, 아이섀도, 치크칼라 및 아이브로우펜슬류로 이루어진 색조화장료 제형; 중에서 선택되는 어느 하나인 것을 특징으로 하는 조성물.Formulation of the cosmetic composition is a cosmetic composition consisting of a lotion, gel, water-soluble liquid, cream, essence, oil-in-water (O / W) type and water-in-oil (W / O) type; Color cosmetic formulations consisting of oil-in-water or water-in-oil makeup bases, foundations, skin covers, lipsticks, lip gloss, face powders, two-way cakes, eye shadows, cheek colors and eyebrow pencils; The composition, characterized in that any one selected from.
  12. 제1항에 있어서,The method of claim 1,
    염증성 피부질환의 예방 또는 치료용 약학 조성물인 것을 특징으로 하는 조성물.A pharmaceutical composition for preventing or treating inflammatory skin diseases.
  13. 제12항에 있어서,The method of claim 12,
    상기 약학 조성물의 제형은 경고제(PLASTERS), 로션제(LPTIONS), 리니멘트제(LINIMENTS), 액제(LIQUIDS AND SOLUTIONS), 에어로솔제(AEROSOLS), 엑스제(EXTRACTS), 연고제(OINTMENTS), 유동엑스제(FLUIDEXTRACTS), 유제(EMULSIONS), 현탁제(SUSPENSIONS), 캅셀제(CAPSULES), 크림제(CREAMS), 연질 또는 경질 젤라틴 캄셀, 첩부제, 서방화제제 중 선택된 어느 하나인 것을 특징으로 하는 조성물.Formulation of the pharmaceutical composition is a PLASTERS, lotion (LPTIONS), linings (LINIMENTS), LIQUIDS AND SOLUTIONS, aerosols (AEROSOLS), EXTRACTS, ointments (OINTMENTS), flow FLUIDEXTRACTS, EMULSIONS, SUSPENSIONS, CAPSULES, CREAMS, soft or hard gelatin campels, patch, sustained release agent .
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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2015095918A1 (en) * 2013-12-24 2015-07-02 Innovative Herbal Products (Aust) Pty Ltd Treatment of pain
US10086033B2 (en) 2013-12-24 2018-10-02 Innovative Herbal Products (Aust) Pty Ltd Treatment of pain

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