WO2012093704A1 - 抗体の物性を改善させる方法 - Google Patents
抗体の物性を改善させる方法 Download PDFInfo
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- WO2012093704A1 WO2012093704A1 PCT/JP2012/050129 JP2012050129W WO2012093704A1 WO 2012093704 A1 WO2012093704 A1 WO 2012093704A1 JP 2012050129 W JP2012050129 W JP 2012050129W WO 2012093704 A1 WO2012093704 A1 WO 2012093704A1
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- polypeptide
- extracellular matrix
- antibody
- binding activity
- physical properties
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
- A61K39/395—Antibodies; Immunoglobulins; Immune serum, e.g. antilymphocytic serum
- A61K39/39591—Stabilisation, fragmentation
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
- G01N33/6854—Immunoglobulins
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P37/00—Drugs for immunological or allergic disorders
- A61P37/02—Immunomodulators
- A61P37/06—Immunosuppressants, e.g. drugs for graft rejection
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
- C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
- C07K16/36—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against blood coagulation factors
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
- A61K2039/505—Medicinal preparations containing antigens or antibodies comprising antibodies
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2317/00—Immunoglobulins specific features
- C07K2317/30—Immunoglobulins specific features characterized by aspects of specificity or valency
- C07K2317/31—Immunoglobulins specific features characterized by aspects of specificity or valency multispecific
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2317/00—Immunoglobulins specific features
- C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
- C07K2317/56—Immunoglobulins specific features characterized by immunoglobulin fragments variable (Fv) region, i.e. VH and/or VL
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2317/00—Immunoglobulins specific features
- C07K2317/90—Immunoglobulins specific features characterized by (pharmaco)kinetic aspects or by stability of the immunoglobulin
- C07K2317/94—Stability, e.g. half-life, pH, temperature or enzyme-resistance
Definitions
- a polypeptide When a polypeptide is administered as a drug, its pharmacokinetics such as plasma kinetics vary depending on the physical properties of the polypeptide. Degradation in plasma (degradation of peptide chain site and degradation of sugar chain site), solubility, antigenicity, metabolism in the liver, plasma proteins (such as albumin) as physical properties that determine the pharmacokinetics of polypeptides
- this is not a limitation.
- Patent Document 1 Investigating using multiple antibody molecules has revealed that the rate of protease degradation in vitro and the blood half-life in vivo are correlated.
- Patent Document 1 There are multiple factors that determine the disposition of a polypeptide in addition to protease resistance, and the influence of various factors on the disposition of a polypeptide varies depending on the type and characteristics of the polypeptide. It is important to examine in detail the correlation between different in vitro parameters and pharmacokinetics.
- Non-patent Document 1 Studies have been made on the blood concentration when VEGF-Trap molecule, which is a fusion protein of VEGF receptor and Fc, is administered subcutaneously.
- VEGF-Trap molecules with high isoelectric points have low bioavailability due to low plasma concentrations when administered subcutaneously.
- a modified VEGF-Trap molecule having a lower isoelectric point by amino acid modification has a higher plasma concentration and improved bioavailability.
- the bioavailability of the VEGF-Trap molecule administered subcutaneously depends on the cell at the subcutaneous site. It has become clear that it depends on the bond strength to the outer matrix.
- the present invention has been made in view of such circumstances, and the object thereof is a method for improving the physical properties of a polypeptide, particularly a method for improving the plasma kinetics of a polypeptide, a method for reducing immunogenicity, or solubility. It is to provide a method for improving the above.
- the present inventors have conducted intensive research on methods for improving the physical properties of polypeptides.
- the present inventors modified the physical properties of the polypeptide, particularly plasma kinetics, immunogenicity, by modifying at least one amino acid residue of the polypeptide so that the binding activity to the extracellular matrix is reduced. And found that the solubility can be improved.
- the present invention relates to a method for improving the physical properties of a polypeptide, particularly a method for improving plasma kinetics or a method for reducing immunogenicity when a polypeptide is administered as a drug, or a case where a polypeptide is used as a solution formulation. More specifically, a method for improving the solubility, a method for producing these polypeptides, a polypeptide produced by the production method, a method for evaluating the physical properties of the polypeptide, and the like.
- [1] A method for improving the physical properties of a polypeptide by reducing the binding activity to the extracellular matrix
- the method according to [1], wherein the improvement of physical properties is at least one selected from improvement of plasma kinetics, reduction of immunogenicity, and improvement of solubility [3] The method according to [1] or [2], wherein reducing the binding activity to the extracellular matrix reduces binding to the extracellular matrix by modifying at least one amino acid residue of the polypeptide, [4] The method according to any one of [1] to [3], wherein the binding activity to the extracellular matrix is measured by an electrochemiluminescence method, [5] The method according to any one of [1] to [4], wherein the polypeptide is a polypeptide having antigen-binding activity.
- a method for producing a polypeptide with improved physical properties comprising a step of modifying at least one amino acid residue of the polypeptide so that the binding activity to the extracellular matrix is reduced
- a method for producing a polypeptide having improved physical properties comprising: (a) modifying at least one amino acid residue of the polypeptide; (b) measuring the binding activity of the polypeptide modified in step (a) to the extracellular matrix; and (c) selecting a polypeptide having a reduced binding activity to the extracellular matrix as compared to that before modification; Manufacturing method including [9] Furthermore, (d) obtaining a gene encoding the polypeptide selected in step (c) of [8], and (e) a step of producing a polypeptide using the gene obtained in the step (d), The production method according to [8], [10] Furthermore, (f) modifying at least one amino acid residue of the polypeptide obtained
- a polypeptide produced by the production method according to any one of [7] to [15], [17] A method for screening a polypeptide having physical properties superior to that of a reference polypeptide, using a polypeptide whose physical properties are known in advance as a reference polypeptide, (a) contacting the reference polypeptide and the test polypeptide or test polypeptide library with an extracellular matrix; (b) measuring the binding activity to the extracellular matrix of the polypeptide contacted in the step (a), and (c) selecting a polypeptide having a lower binding activity to the extracellular matrix than the reference polypeptide from the polypeptides measured in the step (b), Including, method, [18] The screening method according to [17], wherein the improvement in physical properties is at least one selected from improvement in plasma kinetics, reduction in immunogenicity, and improvement in solubility, [19] The screening method according to [17] or [18], wherein the binding activity to the extracellular matrix is measured by an electrochemiluminescence method, [20]
- the physical properties of the test polypeptide are determined. How to evaluate, [23] The method according to [22], wherein the physical property is at least one selected from plasma kinetics, immunogenicity, and solubility of the polypeptide, [24] The method according to [22] or [23], wherein the measurement of the binding activity to the extracellular matrix is measurement by an electrochemiluminescence method, About.
- the present invention provides a method for improving the physical properties of a polypeptide or a method for producing a polypeptide with improved physical properties by reducing the binding of a polypeptide having a certain target function to an extracellular matrix.
- improvement of physical properties of the polypeptide can improve plasma kinetics, reduce immunogenicity, or improve solubility, and can provide a polypeptide suitable for use as a pharmaceutical product. It is also possible to evaluate the physical properties of the test polypeptide by comparing the binding activity of the polypeptide whose physical properties are known in advance to the extracellular matrix and the binding activity of the test polypeptide to the extracellular matrix. .
- FIG. 3 is a graph showing the relationship between extracellular matrix binding of an antibody (MabA1 to A3) and half-life (d) when it is administered to normal mice. It is the figure which measured the extracellular-matrix coupling
- FIG. 3 is a graph showing the results (intravenous administration) of the antibody (MabB1 to B3) plasma dynamics in normal mice evaluated by the method shown in Example 1.
- FIG. 3 is a graph showing the results (subcutaneous administration) of antibody (MabB1-B3) plasma kinetics in normal mice evaluated by the method described in Example 1.
- FIG. 3 is a graph showing the relationship between binding of an antibody (MabC7 to MabC9) to the extracellular matrix and half-life (d) when it is administered to a human FcRn transgenic mouse.
- FIG. 3 is a graph showing extracellular matrix binding of an antibody (MabC1 to MabC6) polypeptide and a T-cell assay reaction rate. It is a figure which shows the relationship between the administration days at the time of administering an antibody (MabB2 and MabB3) and anti-antibody appearance.
- FIG. 3 is a graph showing the relationship between binding of an antibody (MabC7 to MabC9) to the extracellular matrix and half-life (d) when it is administered to a human FcRn transgenic mouse.
- FIG. 3 is a graph showing extracellular matrix binding of an antibody (MabC1 to MabC6) polypeptide and a T-cell assay reaction rate. It is a figure which shows the relationship between the administration days at the time of administering an antibody
- 3 is a graph showing the relationship between the binding of antibodies (MabD1 to MabD30) to the extracellular matrix and solubility. It is a figure which shows the extracellular matrix coupling
- the present invention provides a method for improving the physical properties of a polypeptide. More specifically, a method for improving the physical properties of the polypeptide by reducing the binding activity of the polypeptide to the extracellular matrix is provided. In particular, a method for improving plasma kinetics when a polypeptide is administered in vivo, a method for reducing immunogenicity, or a method for improving solubility when a polypeptide is used as a solution preparation are provided.
- the present invention provides a method for evaluating the physical properties of the test polypeptide by comparing the binding activity of the polypeptide whose physical properties are known in advance to the extracellular matrix and the binding activity of the test polypeptide to the extracellular matrix.
- polypeptide that is the subject of the present invention is not particularly limited, but preferably has a specific function when administered into a living body, and is particularly preferably a polypeptide having an effect as a pharmaceutical product.
- polypeptides include antibodies, hormones, cytokines and the like.
- An example of a polypeptide having the antigen-binding activity of the present invention is an antibody.
- a preferred example of the antibody of the present invention is an IgG antibody.
- an IgG antibody is used as an antibody, the type thereof is not limited, and it is possible to use IgG of an isotype (subclass) such as IgG1, IgG2, IgG3, IgG4.
- the polypeptide of the present invention may contain an antibody constant region, and amino acid mutations may be introduced into the constant region.
- the antibody may be an antibody derived from any animal such as a mouse antibody, a human antibody, a rat antibody, a rabbit antibody, a goat antibody, or a camel antibody.
- a chimeric antibody particularly a modified antibody with a substituted amino acid sequence such as a humanized antibody may be used.
- it may be a bispecific antibody, a modified antibody obtained by binding various molecules, a polypeptide containing an antibody fragment, and the like.
- a “chimeric antibody” is an antibody produced by combining sequences derived from different animals. Specific examples of the chimeric antibody include an antibody consisting of a mouse antibody heavy chain and light chain variable (V) region and a human antibody heavy chain and light chain constant (C) region.
- V mouse antibody heavy chain and light chain variable
- C human antibody heavy chain and light chain constant
- Humanized antibody refers to an antibody derived from a mammal other than a human, also referred to as a reshaped human antibody, such as a complementarity determination region (CDR) of a mouse antibody to the CDR of a human antibody. It is transplanted.
- CDR complementarity determination region
- Methods for identifying CDRs are known (Kabat et al., Sequence of Proteins of Immunological Interest (1987), National Institute of Health, Bethesda, Md .; Chothia et al., Nature (1989) 342: 877) .
- general gene recombination techniques are also known (see European Patent Application Publication No. EP-125023 and WO96 / 02576).
- the C region of these antibodies is preferably derived from a human antibody.
- C ⁇ 1, C ⁇ 2, C ⁇ 3, C ⁇ 4, etc. can be used for the H chain
- C ⁇ , C ⁇ , etc. can be used for the L chain.
- amino acid mutations may be introduced into the human antibody C region as necessary in order to improve antibody stability or antibody production.
- the chimeric antibody in the present invention preferably comprises a variable region of a non-human mammal-derived antibody and a constant region derived from a human antibody.
- humanized antibodies are preferably composed of CDRs of antibodies derived from mammals other than humans, and FR and C regions derived from human antibodies.
- the constant region derived from a human antibody preferably contains a human FcRn binding region, and examples of such an antibody include IgG (IgG1, IgG2, IgG3, IgG4).
- the constant region used for the humanized antibody in the present invention may be a constant region of an antibody belonging to any isotype.
- a constant region derived from human IgG1 is used, but is not limited thereto.
- the FR derived from a human antibody used for a humanized antibody is not particularly limited, and may be an antibody belonging to any isotype.
- variable region and constant region of the chimeric antibody and humanized antibody in the present invention may be modified by deletion, substitution, insertion and / or addition as long as they show the binding specificity of the original antibody.
- Bispecific antibody refers to an antibody having variable regions that recognize different epitopes in the same antibody molecule.
- the bispecific antibody may be an antibody that recognizes two or more different antigens, or may be an antibody that recognizes two or more different epitopes on the same antigen.
- polypeptides containing antibody fragments include Fab fragments, F (ab ′) 2 fragments, scFv (Nat Biotechnol. 2005 Sep; 23 (9): 1126-36.) Domain antibody (dAb) (WO2004 / 058821, WO2003 / 002609), scFv-Fc (WO2005 / 037989), dAb-Fc, Fc fusion polypeptide and the like.
- Molecules containing the Fc region can use the Fc region as a human FcRn binding domain. These molecules may be fused with a human FcRn binding domain.
- the polypeptide in the present invention may be an antibody-like molecule.
- Antibody-like molecules are molecules that exert their functions by binding to target molecules (Current Opinion in Biotechnology 2006, 17: 653-658, Current Opinion in Biotechnology 2007, 18: 1-10, Current Opinion in Structural Biology 1997, 7: 463-469, Protein Science 2006, 15: 14-27), for example, DARPins (WO2002 / 020565), Affibody (WO1995 / 001937), Avimer (WO2004 / 044011, WO2005 / 040229), Adnectin (WO2002 / 032925) and the like. Even these antibody-like molecules can improve the physical properties of the antibody molecule by reducing the binding activity to the extracellular matrix.
- the “physical properties” of a polypeptide means plasma kinetics and immunogenicity when the polypeptide is administered in vivo, or solubility when the polypeptide is used as a solution preparation.
- “improves physical properties” is only required to improve any of these physical properties, and is particularly important when a polypeptide is used as a pharmaceutical product. Plasma kinetics, immunogenicity, and solubility are important. It is preferable that any one of these is improved.
- the method for measuring “binding to the extracellular matrix” is not particularly limited, but the polypeptide is added to a plate on which the extracellular matrix is immobilized, and a labeled antibody against the polypeptide is added, thereby adding a polypeptide. It can be measured by an ELISA system that detects the binding of peptide and extracellular matrix.
- a measurement method using an electrochemiluminescence method (ECL (Electrochemiluminescence)) is preferable because it is possible to detect extracellular matrix binding ability with higher sensitivity.
- a mixture of a polypeptide and a ruthenium antibody is added to a plate on which an extracellular matrix is immobilized, and the binding between the polypeptide and the extracellular matrix is measured by an ECL system that measures the electrochemiluminescence of ruthenium.
- the addition concentration of the polypeptide can be arbitrarily set, and the addition concentration is preferably higher in order to increase the detection sensitivity for binding to the extracellular matrix.
- the extracellular matrix used in the present invention may be animal-derived or plant-derived as long as it contains glycoproteins such as collagen, proteoglycan, fibronectin, laminin, entactin, fibrin, perlecan, etc.
- an extracellular matrix derived from an animal such as human, mouse, rat, monkey, rabbit, or dog can be used.
- human-derived natural extracellular matrix is preferred for monitoring the improvement in plasma kinetics in humans.
- a neutral range around pH 7.4, which is a physiological condition is desirable, but it is not necessarily a neutral range, and an acidic range (pH 6.0). It may be evaluated in the vicinity).
- the binding of the polypeptide-antigen molecule complex to the extracellular matrix can also be evaluated by coexisting the antigen molecule to which the polypeptide binds. Good.
- “improving plasma kinetics” means “improving plasma pharmacokinetics”, “improving plasma retention”, “excellent plasma retention”, “prolonging plasma retention”. In other words, these terms are used interchangeably.
- “improving plasma kinetics” means that the polypeptide of the present invention is administered to animals such as humans, mice, rats, monkeys, rabbits, dogs and the like until it disappears from plasma (for example, intracellularly). This means that the time until the polypeptide of the present invention becomes impossible to return to plasma due to degradation in the reaction time becomes longer.
- “improving plasma dynamics” means any parameter such as an increase in plasma half-life, an increase in mean plasma residence time, or a decrease in plasma clearance (understanding by pharmacokinetic exercises (Nanzan-do)) It is possible to confirm the improvement of plasma kinetics by measuring.
- the plasma concentration of the polypeptide is measured, each parameter is calculated, and the plasma half-life is increased.
- the average plasma residence time is increased, it can be said that the plasma kinetics of the polypeptide has improved.
- These parameters can be measured by methods known to those skilled in the art, and can be appropriately evaluated by, for example, noncompartmental analysis using the pharmacokinetic analysis software WinNonlin (Pharsight) according to the attached procedure manual.
- plasma dynamics in humans be improved. If it is difficult to measure plasma dynamics in humans, use mice (eg, normal mice, human antigen-expressing transgenic mice, human FcRn-expressing transgenic mice, etc.) and monkeys (eg, cynomolgus monkeys).
- mice eg, normal mice, human antigen-expressing transgenic mice, human FcRn-expressing transgenic mice, etc.
- monkeys eg, cynomolgus monkeys.
- the plasma kinetics in humans can be predicted based on the plasma kinetics of.
- “reduction of immunogenicity” means that cell proliferation and production of inflammatory cytokines are suppressed when the polypeptide of the present invention is added to immune cells in vitro, or in vivo. It means a reduction in the appearance frequency and strength of an antibody against the polypeptide when administered.
- cell growth when a polypeptide is added to PBMC in vitro is a newly synthesized DNA in a cell in which BrdU (bromodeoxyuridine), a blue colored substrate, is actively proliferating. Can be measured by a spectrophotometer (Baker MP, Carr FJ.Curr Drug Saf. 2010, 5 (4): 308-13.).
- antibodies that appear when a polypeptide is administered in vivo in vivo are detected by methods known to those skilled in the art, such as a Sandwich-ELISA using a polypeptide or an ELISA system using a plate on which the polypeptide is immobilized. Can be measured. Cytokine production can be measured by a method using Sandwich ELISA using an antibody against the cytokine. More specifically, immunogenicity can be measured according to the method as described in Example 4.
- “improving solubility” means solubility in a buffer in the presence of polyethylene glycol (PEG) (Guiotto, A. et al. (2004) Bioorg. Med. Chem. 12, 5031-5037), and This means that the measured solubility in the buffer is increased.
- PEG polyethylene glycol
- the type of buffer for measuring the solubility can be arbitrarily selected. Specifically, for example, the solubility can be measured according to the method as described in Example 6.
- the amino acid residue modification that reduces the binding of the polypeptide of the present invention to the extracellular matrix is not particularly limited, but in the present invention, the amino acid on the surface of the polypeptide having antigen-binding activity is modified. It is preferable to do.
- the binding to the extracellular matrix is reduced by modifying at least one amino acid residue of the polypeptide. At that time, it is preferable to consider so as to reduce the influence of the modification on other activities of the polypeptide.
- the polypeptide of the present invention is an antibody
- the binding to the extracellular matrix mainly involves residues exposed on the surface of the antibody variable region
- the surface of the antibody It is preferable to modify the amino acid of the variable region exposed to.
- the variable region amino acids exposed on the surface of the antibody can be found by methods known to those skilled in the art using an antibody homology model.
- the modification of amino acid residues can be performed by modifying one or more bases in DNA encoding a polypeptide and expressing the DNA in a host cell as described below.
- a person skilled in the art can easily determine the number, position, and type of bases to be modified according to the type of amino acid residue after modification.
- alteration means any one of substitution, deletion, addition, insertion, or a combination thereof.
- base modification refers to genetic manipulation or mutation that inserts, deletes, or substitutes at least one base in DNA so that the polypeptide encoded by DNA has the target amino acid residue. It means to process. That is, conversion of a codon encoding the original amino acid residue into a codon encoding the target amino acid residue is included.
- base modification can be carried out by site-directed mutation (for example, see Kunkel (1985) Proc. Natl. Acad. Sci. USA 82: 488), PCR mutagenesis, cassette mutation, and the like .
- antibody variants with improved biological properties are 70% or more, more preferably 80% or more, still more preferably 90% or more (e.g.
- sequence homology and / or similarity is homologous to the original antibody residues after aligning the sequences and introducing gaps as necessary to maximize sequence homology. Defined as the percentage of amino acid residues that are (same residues) or similar (amino acid residues that are grouped in the same group based on the properties of the side chains of common amino acids).
- Natural amino acid residues are usually based on their side chain properties (1) hydrophobicity: alanine, isoleucine, valine, methionine and leucine; (2) neutral hydrophilicity: asparagine, glutamine, cysteine, threonine and serine. (3) acidity: aspartic acid and glutamic acid; (4) basicity: arginine, histidine and lysine; (5) residues affecting chain orientation: glycine and proline; and (6) aromaticity: tyrosine, tryptophan. And phenylalanine group.
- the DNA encoding the polypeptide thus modified is cloned (inserted) into an appropriate vector and introduced into a host cell.
- the vector is not particularly limited as long as it can stably hold a nucleic acid such as inserted DNA.
- Escherichia coli is used as a host, a cloning vector may be mentioned.
- the cloning vector is preferably a pBluescript vector (Stratagene), but various commercially available vectors can be used.
- an expression vector is particularly useful.
- the expression vector is not particularly limited as long as it is a vector that expresses a polypeptide in a test tube, E. coli, cultured cells, or an individual organism.
- pBEST vector Promega
- pET vector Invitrogen
- pME18S-FL3 vector GenBank ⁇ Accession No. AB009864
- the pME18S vector is preferred.
- DNA can be inserted into a vector by a conventional method, for example, by a ligase reaction using a restriction enzyme site (Current (protocols in Molecular Biology edit. Ausubel et al. (1987) Publish. John Wiley & Sons. Section 11.4-11.11).
- the host cell is not particularly limited, and various host cells can be used depending on the purpose.
- Examples of cells for expressing the polypeptide include bacterial cells (eg, Streptococcus, Staphylococcus, E. coli, Streptomyces, Bacillus subtilis), fungal cells (eg, yeast, Aspergillus), insect cells (eg, Drosophila S2). Spodoptera SF9), animal cells (eg, CHO, COS, HeLa, C127, 3T3, BHK, HEK293, BowesB melanoma cells) and plant cells.
- Vector introduction into host cells can be performed by, for example, calcium phosphate precipitation method, electric pulse perforation method (Current protocols in Molecular Biology edit. Ausubel et al. (1987) Publish. John Wiley & SSons.Section 9.1-9.9), lipofection method, micro It can be performed by a known method such as an injection method.
- an appropriate secretion signal can be incorporated into the polypeptide of interest.
- These signals may be endogenous to the polypeptide of interest or may be heterologous signals.
- the expression product is recovered when the polypeptide is secreted into the medium. If the polypeptide is produced intracellularly, the cell is first lysed and then the polypeptide is recovered.
- ammonium sulfate or ethanol precipitation acid extraction, anion or cation exchange chromatography, phosphocellulose chromatography, hydrophobic interaction chromatography, affinity chromatography, hydroxylapatite chromatography
- Known methods including graphy and lectin chromatography can be used.
- the polypeptide with improved physical properties of the present invention defines a polypeptide as a standard for physical properties, and a polypeptide having an extracellular matrix binding ability lower than the ability of the polypeptide to bind to the extracellular matrix is present in advance.
- the amino acid modification can be performed according to the above description.
- the method for evaluating improvement in physical properties can be carried out in accordance with the method described in the examples.
- the present invention provides a method for producing a polypeptide with improved physical properties. That is, a method for producing a polypeptide having a reduced binding activity to the extracellular matrix is provided.
- the present invention also provides a method for producing a polypeptide with improved plasma kinetics, reduced immunogenicity, or improved solubility by reducing the binding activity to the extracellular matrix.
- the present invention provides a method for producing a polypeptide having an antigen binding activity, particularly an antibody, which is particularly useful when used as a pharmaceutical composition.
- the present invention provides a method for producing a polypeptide comprising the following steps; (a) modifying at least one amino acid residue of the polypeptide; (b) measuring the binding activity of the polypeptide modified in step (a) to the extracellular matrix; and (c) A step of selecting a polypeptide having a reduced binding activity to the extracellular matrix compared to that before modification.
- steps (a) and (b) may be repeated two or more times. The number of times the steps (a) and (b) are repeated is not particularly limited, but is usually within 10 times.
- the manufacturing method described above may include the following steps. (d) obtaining a gene encoding the polypeptide selected in step (c), and (e) A step of producing a polypeptide using the gene obtained in the step (d).
- the above manufacturing method can further include the following steps.
- (f) A step of modifying at least one amino acid residue of the polypeptide obtained in the step (e) and repeatedly performing the steps (b) to (e). It is repeated in the step (f) (
- the number of steps b)-(e) is not particularly limited, but is usually within 10 times.
- the present invention provides a method for producing a polypeptide having better physical properties than the polypeptide, based on a polypeptide whose physical properties are known in advance. That is, using the binding activity of the polypeptide to the extracellular matrix as an index, the plasma kinetics are improved and the immunogenicity is reduced compared to the polypeptide with known plasma kinetics, immunogenicity or solubility. Alternatively, a method for producing a polypeptide with improved solubility is provided. Furthermore, the present invention provides a method for producing a polypeptide having an antigen binding activity, particularly an antibody, which is particularly useful when used as a pharmaceutical composition.
- the present invention is a method for producing a polypeptide having better physical properties than the reference polypeptide using a polypeptide whose physical properties are known in advance as a reference polypeptide, and comprising the following steps: Providing a manufacturing method; (a) contacting the reference polypeptide and the test polypeptide or test polypeptide library with an extracellular matrix; (b) measuring the binding activity to the extracellular matrix of the polypeptide contacted in the step (a), (c) selecting a polypeptide having a lower binding activity to the extracellular matrix than the reference polypeptide from the polypeptides measured in the step (b), (d) obtaining a gene encoding the polypeptide selected in step (c), and (e) A step of producing a polypeptide using the gene obtained in the step (d).
- the steps (a) to (c) may be repeated two or more times. The number of times the steps (a) to (c) are repeated is not particularly limited, but is usually within 10 times.
- the present invention provides a method of screening for a polypeptide having better physical properties than the polypeptide, based on a polypeptide whose physical properties are known in advance. That is, using the binding activity of the polypeptide to the extracellular matrix as an index, the plasma kinetics are improved and the immunogenicity is reduced compared to the polypeptide with known plasma kinetics, immunogenicity or solubility. Or a method of screening for polypeptides with improved solubility. Furthermore, the present invention provides a method for producing a polypeptide having an antigen binding activity, particularly an antibody, which is particularly useful when used as a pharmaceutical composition.
- the present invention is a method for screening a polypeptide having a physical property superior to that of the reference polypeptide using a polypeptide whose physical properties are known in advance as a reference polypeptide, the polypeptide comprising the following steps: Providing a screening method; (a) contacting the reference polypeptide and the test polypeptide or test polypeptide library with an extracellular matrix; (b) measuring the binding activity to the extracellular matrix of the polypeptide contacted in the step (a), and (c) A step of selecting a polypeptide having a lower binding activity to the extracellular matrix than the reference polypeptide from the polypeptides measured in the step (b).
- the steps (a) to (c) may be repeated two or more times. The number of times the steps (a) to (c) are repeated is not particularly limited, but is usually within 10 times.
- Polypeptides used in the production method and screening method of the present invention may be prepared in any way, for example, preexisting antibodies, preexisting libraries (such as phage libraries), immunization to animals It is possible to use antibodies or libraries prepared from hybridomas obtained from B cells from immunized animals or libraries, antibodies or libraries in which amino acids are randomly altered in these antibodies or libraries, and the like.
- the polypeptide produced by the production method of the present invention or the polypeptide obtained by the screening method of the present invention is improved in plasma kinetics and immunogenicity when administered to animals such as humans, mice and monkeys. It is considered possible to reduce the solubility and improve the solubility when a solution preparation is made. Therefore, the production method of the present invention can be used as a method for producing or screening a polypeptide with improved plasma kinetics, reduced immunogenicity, or improved solubility.
- these polypeptides produced by the production method of the present invention or the polypeptides obtained by the screening method of the present invention are compared with the plasma before the binding activity to the extracellular matrix is modified. It is considered to be particularly excellent when used as a pharmaceutical because it is a polypeptide with improved medium kinetics, reduced immunogenicity, and improved solubility. Therefore, the production method of the present invention can be used as a method for producing a polypeptide for use as a pharmaceutical composition.
- the screening method of the present invention can easily select a polypeptide having excellent plasma kinetics, immunogenicity, and solubility by using the binding activity to the extracellular matrix as an index.
- it can be used for obtaining a polypeptide suitable for a pharmaceutical product.
- the polypeptide obtained by the production method of the present invention or the DNA encoding the polypeptide obtained by the screening method of the present invention is usually carried (inserted) into an appropriate vector and introduced into a host cell.
- the vector is not particularly limited as long as it stably retains nucleic acid such as inserted DNA.
- E. coli is used as a host
- a pBluescript vector (Stratagene) is preferable as a cloning vector.
- An expression vector is particularly useful when a vector is used for the purpose of producing the polypeptide of the present invention.
- the expression vector is not particularly limited as long as it is a vector that expresses a polypeptide in vitro, in E.
- coli in cultured cells, or in an individual organism.
- pBEST vector manufactured by Promega
- E. coli PET vector manufactured by Invitrogen
- pME18S-FL3 vector GenBank Accession No. AB009864
- pME18S vector Mol Cell Biol. 8: 466-472 (1988)
- the DNA of the present invention can be inserted into a vector by a conventional method, for example, by a ligase reaction using a restriction enzyme site (Current ⁇ ⁇ ⁇ protocols in Molecular Biology edit. Ausubel et al. (1987) Publish. John Wiley & Sons, Section IV 11.4-11.11.
- the host cell is not particularly limited, and various host cells can be used depending on the purpose.
- Examples of cells for expressing the polypeptide include bacterial cells (eg, Streptococcus, Staphylococcus, E. coli, Streptomyces, Bacillus subtilis), fungal cells (eg, yeast, Aspergillus), insect cells (eg, Drosophila S2). Spodoptera SF9), animal cells (eg, CHO, COS, HeLa, C127, 3T3, BHK, HEK293, BowesB melanoma cells) and plant cells.
- Vector introduction into host cells can be performed by, for example, calcium phosphate precipitation method, electric pulse perforation method (Current protocols in Molecular Biology edit. Ausubel et al. (1987) Publish. John Wiley & SSons.Section 9.1-9.9), lipofection method, micro It can be performed by a known method such as an injection method.
- Host cell culture can be performed according to a known method. For example, when animal cells are used as hosts, for example, DMEM, MEM, RPMI1640, and IMDM can be used as a culture solution. At that time, the cells may be cultured by serum-free culture, even if serum supplements such as FBS and fetal calf serum (FCS) are used in combination.
- the pH during culture is preferably about 6-8.
- the culture is usually performed at about 30 to 40 ° C. for about 15 to 200 hours, and medium exchange, aeration, and agitation are added as necessary.
- an appropriate secretion signal can be incorporated into the polypeptide of interest.
- These signals may be endogenous to the polypeptide of interest or may be heterologous signals.
- examples of systems that produce polypeptides in vivo include production systems that use animals and production systems that use plants.
- a DNA encoding a target polypeptide is introduced into these animals or plants, and the polypeptide is produced in the body of the animal or plant and recovered.
- the “host” in the present invention includes these animals and plants.
- mammals there are production systems using mammals and insects.
- mammals goats, pigs, sheep, mice, cows and the like can be used (Vicki Glaser, SPECTRUM Biotechnology Applications (1993)).
- a transgenic animal can be used.
- a DNA encoding the polypeptide of the present invention is prepared as a fusion gene with a DNA encoding a polypeptide inherently produced in milk such as goat ⁇ casein.
- a DNA fragment containing the fusion gene is injected into a goat embryo, and the embryo is transferred to a female goat.
- the polypeptide of interest can be obtained from the milk produced by a transgenic goat born from a goat that has received the embryo or its offspring.
- hormones may be administered to the transgenic goat as appropriate (Ebert et al., Bio / Technology (1994) 12: 699-702).
- the target polypeptide can be obtained from the body fluid of the silkworm by infecting the silkworm with a baculovirus into which a DNA encoding the target polypeptide is inserted.
- a plant when a plant is used for producing the polypeptide of the present invention, for example, tobacco can be used.
- tobacco a DNA encoding a desired polypeptide is inserted into a plant expression vector such as pMON 530, and this vector is introduced into a bacterium such as Agrobacterium tumefaciens.
- This bacterium can be infected with tobacco, for example, Nicotiana tabacum, and the desired polypeptide can be obtained from the leaves of this tobacco (Ma et al., Eur. J. Immunol. (1994) 24: 131). -8).
- a desired polypeptide can be obtained from a duckweed cell (Cox KM et al. Nat. Biotechnol. 2006 Dec; 24 (12): 1591-1597).
- polypeptide thus obtained can be isolated from inside or outside the host cell (medium, milk, etc.) and purified as a substantially pure and homogeneous polypeptide. Separation and purification of polypeptides may be carried out using separation and purification methods used in usual polypeptide purification, and are not limited in any way. For example, chromatography column, filter, ultrafiltration, salting out, solvent precipitation, solvent extraction, distillation, immunoprecipitation, SDS-polyacrylamide gel electrophoresis, isoelectric focusing, dialysis, recrystallization, etc. are appropriately selected, In combination, polypeptides can be separated and purified.
- chromatography examples include affinity chromatography, ion exchange chromatography, hydrophobic chromatography, gel filtration, reverse phase chromatography, adsorption chromatography, etc. (Strategies for Protein Purification and Characterization: A Laboratory Course Manual. Ed Daniel R. Marshak et al. (1996) Cold Spring Harbor Laboratory Press). These chromatography can be performed using liquid phase chromatography, for example, liquid phase chromatography such as HPLC and FPLC.
- Columns used for affinity chromatography include protein A columns and protein G columns. Examples of the column using protein A include Hyper D, POROS, Sepharose F F F (Pharmacia), and the like.
- an appropriate protein modifying enzyme can be allowed to act before or after purification of the polypeptide to arbitrarily modify it or remove the peptide partially.
- protein modifying enzymes include trypsin, chymotrypsin, lysyl endopeptidase, protein kinase, glucosidase, and the like.
- the present invention also relates to a pharmaceutical composition
- a pharmaceutical composition comprising a polypeptide obtained by the production method or screening method of the present invention.
- the polypeptide of the present invention, the polypeptide produced by the production method of the present invention, or the polypeptide obtained by the screening method of the present invention is improved in plasma kinetics and immunogenicity compared to normal antibodies by its administration. It is useful as a pharmaceutical composition because it is considered that the solubility of a solution preparation can be improved.
- the pharmaceutical composition of the present invention may comprise a pharmaceutically acceptable carrier.
- the pharmaceutical composition usually refers to a drug for treatment or prevention of a disease, or examination / diagnosis.
- the pharmaceutical composition of the present invention can be formulated by methods known to those skilled in the art. For example, it can be used parenterally in the form of a sterile solution with water or other pharmaceutically acceptable liquid, or an injection of suspension.
- a pharmacologically acceptable carrier or medium specifically, sterile water or physiological saline, vegetable oil, emulsifier, suspension, surfactant, stabilizer, flavoring agent, excipient, vehicle, preservative
- a pharmaceutical preparation by combining with a binder or the like as appropriate and mixing in a unit dosage form generally required for pharmaceutical practice. The amount of the active ingredient in these preparations is set so as to obtain an appropriate volume within the indicated range.
- a sterile composition for injection can be formulated in accordance with normal pharmaceutical practice using a vehicle such as distilled water for injection.
- a vehicle such as distilled water for injection.
- the aqueous solution for injection include isotonic solutions containing physiological saline, glucose and other adjuvants (for example, D-sorbitol, D-mannose, D-mannitol, sodium chloride).
- a suitable solubilizing agent such as alcohol (ethanol etc.), polyalcohol (propylene glycol, polyethylene glycol etc.), nonionic surfactant (polysorbate 80 (TM), HCO-50 etc.) may be used in combination.
- oily liquid examples include sesame oil and soybean oil, and benzyl benzoate and / or benzyl alcohol may be used in combination as a solubilizing agent.
- blend with a buffering agent for example, phosphate buffer and sodium acetate buffer
- a soothing agent for example, procaine hydrochloride
- a stabilizer for example, benzyl alcohol and phenol
- antioxidant for example, benzyl alcohol and phenol
- composition of the present invention is preferably administered by parenteral administration.
- the composition can be an injection, nasal, pulmonary, or transdermal composition.
- it can be administered systemically or locally by intravenous injection, intramuscular injection, intraperitoneal injection, subcutaneous injection, or the like.
- the administration method can be appropriately selected depending on the age and symptoms of the patient.
- the dosage of the pharmaceutical composition containing the polypeptide of the present invention can be set, for example, in the range of 0.0001 mg to 1000 mg per kg body weight. Alternatively, for example, the dose may be 0.001 to 100,000 mg per patient, but the present invention is not necessarily limited to these values.
- the dose and administration method vary depending on the patient's weight, age, symptoms, etc., but those skilled in the art can set an appropriate dose and administration method in consideration of these conditions.
- the pharmaceutical composition of the present invention can be a pharmaceutical composition used for improving plasma kinetics, reducing immunogenicity, or improving the solubility in a solution formulation.
- the present invention also relates to a method for improving plasma kinetics or immunogen by administering to a subject the polypeptide of the present invention, the polypeptide produced by the production method of the present invention, or the pharmaceutical composition of the present invention.
- the present invention relates to a method for reducing the property. Administration may be performed either in vivo or in vitro. Examples of administration subjects include non-human animals (such as mice and monkeys) and humans.
- amino acids contained in the amino acid sequences described in the present invention are modified after translation (for example, modification to pyroglutamic acid by pyroglutamylation of N-terminal glutamine is a modification well known to those skilled in the art). In some cases, even if the amino acid is post-translationally modified as such, it is naturally included in the amino acid sequence described in the present invention.
- Example 1 Evaluation of association between extracellular matrix binding and pharmacokinetics in normal mouse plasma MabA1, A2, and A3 target blood coagulation factor IX and / or activated blood coagulation factor IX and blood coagulation factor X
- target blood coagulation factor IX and / or activated blood coagulation factor IX and blood coagulation factor X These are bispecific antibodies that exhibit specific binding as an antigen and exhibit blood coagulation factor VIII-like clotting activity.
- These antibodies can be used by those skilled in the art using the methods described in WO2005 / 035756 and WO2006 / 109592. It is an IgG antibody obtained by screening using a known antibody obtaining technique using the binding to the target antigen and the coagulation activity as an index, and further adding an amino acid modification that improves blood coagulation factor VIII-like coagulation activity.
- MabA1, A2, and A3 were administered once to normal mice (C57BL / 6J, Charles River Japan) at 1 mg / kg, and blood was collected in a timely manner. The collected blood was immediately centrifuged at 4 ° C. and 15,000 rpm for 15 minutes to obtain plasma. The separated plasma was stored in a freezer set to ⁇ 20 ° C. or lower until measurement was performed.
- the mouse plasma concentration was measured by ELISA.
- Anti-Human IgG ( ⁇ ) was dispensed into a 96-well plate and allowed to stand overnight at 4 ° C. to prepare an Anti-Human IgG ( ⁇ ) -immobilized plate.
- a calibration curve sample and a mouse plasma measurement sample were prepared, dispensed onto an Anti-Human IgG ( ⁇ ) solid phase plate, and allowed to stand at room temperature for 1 hour.
- Anti-human IgG-AP SIGMA
- the mouse plasma concentration was calculated from the absorbance of the calibration curve using the analysis software SOFTmax PRO (Molecular Devices).
- a plot of MabA1, A2, A3 extracellular matrix binding and plasma half-life (T1 / 2) is shown in FIG.
- T1 / 2 A plot of MabA1, A2, A3 extracellular matrix binding and plasma half-life (T1 / 2) is shown in FIG.
- T1 / 2 extracellular matrix binding and plasma half-life (T1 / 2) are correlated, and extracellular matrix binding measurement is a suitable method for predicting plasma pharmacokinetics. It was suggested that it is possible to improve the plasma pharmacokinetics (plasma half-life) of the antibody by reducing it.
- Example 2 Sensitivity of detection of extracellular matrix binding and improvement of plasma retention of MabB1 by amino acid substitution
- MabB1 is the same method as in Example 1, and blood coagulation factor IX is performed by methods known to those skilled in the art. And / or activated blood coagulation factor IX, and antibodies obtained by performing screening using as an index the binding of both blood coagulation factor X to the target antigen and blood coagulation factor VIII-like coagulation activity, IgG antibody produced by amino acid substitution to improve blood coagulation factor VIII-like coagulation activity.
- MabB1 has a very short plasma half-life, and a short plasma half-life is a major challenge in developing MabB1 as a pharmaceutical because it leads to increased dose and shorter dose intervals.
- MabB1 had a high extracellular matrix binding ability
- various modified MabB1 antibodies in which amino acid substitutions were introduced into residues exposed on the surface of the antibody variable region were prepared for MabB1 by methods known to those skilled in the art, Screening for binding to the extracellular matrix was performed.
- MabB2 and MabB3 with reduced extracellular matrix binding ability were obtained.
- FIG. 2 shows the results of measuring the extracellular matrix binding of the antibody by the color development ELISA method described above.
- MabB1 showed strong binding to extracellular matrix binding, but MabB2 and MabB3 into which amino acid substitution was introduced no longer showed binding to extracellular matrix binding.
- MSD Read Buffer T (4x) manufactured by MSD was added, and the amount of luminescence was measured with SECTOR PR400.
- FIG. 2 The result of measuring the extracellular matrix binding of the antibody (MabB2 / MabB3) by the above method is shown in FIG.
- ELISA MabB2 extracellular matrix binding could not be detected (FIG. 2), but in the high sensitivity system using ECL, MabB2 extracellular matrix binding could be confirmed. That is, by using ECL, it was shown that the extracellular matrix binding of MabB1, MabB2, and MabB3 correlated with their plasma retention.
- Example 3 Improvement of plasma retention of MabC9 by reducing extracellular matrix binding MabC9 was treated in the same manner as in Example 1 by methods known to those skilled in the art, and blood coagulation factor IX and / or activated blood coagulation. Blood coagulation factor VIII-like coagulation against antibodies obtained by screening with factor IX and the binding of both blood coagulation factor X to the target antigen and blood coagulation factor VIII-like coagulation activity IgG antibody produced by amino acid substitution to improve activity. Since MabC9 was found to have a very short plasma half-life, it was investigated to improve the plasma pharmacokinetics of MabC9. As a result, MabC9 was confirmed to bind strongly to the extracellular matrix, and the short plasma half-life was considered to be caused by nonspecific binding to the extracellular matrix.
- MabC9 had a high extracellular matrix binding ability
- various modified MabC9 antibodies in which amino acid substitutions were introduced into residues exposed on the surface of the antibody variable region were prepared in MabC9 by methods known to those skilled in the art, Screening for binding to the extracellular matrix was performed.
- MabC8 and MabC7 with reduced extracellular matrix binding ability were obtained.
- MabC9, MabC8, and MabC7 were intravenously administered to human FcRn transgenic mice (B6.mFcRn ⁇ / ⁇ . HFcRn Tg line 276 + / + mice, Jackson Laboratories) at 1 mg / kg to evaluate plasma pharmacokinetics.
- FIG. 6 it was found that MabC8 and MabC7 with reduced extracellular matrix binding ability significantly increased plasma half-life and improved pharmacokinetics compared to MabC9. .
- Example 4 Evaluation of association between extracellular matrix binding and immunogenicity MabC1 to C6 were prepared in the same manner as in Example 1, but by methods known to those skilled in the art, blood coagulation factor IX and / or activated blood coagulation factor Blood clotting factor VIII-like clotting activity against antibodies obtained by screening with the binding of both factor IX and blood clotting factor X to target antigen and blood clotting factor VIII-like clotting activity as indicators IgG antibody produced by amino acid substitution to improve As a result of evaluating the binding of MabC1 to C6 to the extracellular matrix by ECL, it was found that the degree of binding of these antibodies to the extracellular matrix varies depending on the antibody.
- the antibody that binds to the extracellular matrix exhibits non-specific binding to other than the target antigen, it may also bind to antigen-presenting cells. Therefore, it is considered that the antibody that binds to the extracellular matrix is easily taken up by the antigen-presenting cell, thereby easily presenting the antigen, and the immunogenicity may be increased. Immunogenicity is an important issue for protein drugs, and it is desirable that protein drugs have low immunogenicity.
- CD8 negative CD25 low positive peripheral blood mononuclear cells CD8 - CD25 low PBMCs
- CD8 - CD25 low PBMCs CD8 negative CD25 low positive peripheral blood mononuclear cells separated from fresh human blood were seeded in a 24-well plate and pre-cultured at 37 ° C. and 5% CO 2 in a humidified condition for about 2 hours. After pre-culture, the antibody was added, and the cells were cultured at 37 ° C. and 5% CO 2 wet conditions. Cells collected on the 6th, 7th, and 8th days of culture were seeded in a 96-well plate and Bromodeoxyuridine (BrdU) was added.
- PrdU Bromodeoxyuridine
- Helper T cells incorporating BrdU were analyzed by flow cytometry. From the ratio of BrdU positive cells in Helper T cells, the degree of proliferation activity for each antibody was examined. The experiment was performed with a plurality of donors, and it was evaluated how many percent of each antibody exhibited proliferative activity. The higher the proportion of responding donors, the higher the immunogenicity.
- FIG. 7 shows the reaction rate of donors showing proliferation activity on the horizontal axis and the extracellular matrix binding plotted on the vertical axis.
- Example 5 Reduction of MabB2 immunogenicity by reducing extracellular matrix binding MabB2 prepared in Example 2 is highly immunogenic because anti-MabB2 mouse antibodies are produced in a short period of time when administered to mice. It has been confirmed. In Example 4, it was suggested that an antibody having a lower extracellular matrix binding ability has a lower immunogenicity, and it is possible to reduce the immunogenicity of the antibody by reducing the extracellular matrix binding ability. In the evaluation of plasma retention in mice of MabB2 and MabB3, anti-MabB2 antibody and anti-MabB3 antibody in mouse plasma were detected by ECL immunoassay.
- the antibody was converted to Biotin using EZ-Link TM Sulfo-NFS-Biotinylation Kit (PIERCE) and sTag using Sulfo-Tag NHS Ester 150 nmol (MSD).
- a mouse plasma measurement sample, a biotinylated antibody and an sTag antibody were mixed, and allowed to stand overnight at 4 ° C. This sample was dispensed onto a MULTI-ARRAY® PR SA Plate (manufactured by MSD) and shaken at room temperature for 2 hours. Thereafter, MSD Read Buffer T (4x) (manufactured by MSD) was added, and the luminescence was measured with SECTOR PR (R) 400.
- FIG. MabB3 A plot of elapsed time (days) on the horizontal axis and antibody titer (ECL signal) on the vertical axis is shown in FIG. MabB3 with reduced extracellular matrix binding ability was confirmed to have a lower antibody titer and reduced immunogenicity compared to MabB2.
- Example 6 Evaluation of association between extracellular matrix binding and solubility MabD1 to D30 were prepared in the same manner as in Example 1, and by methods known to those skilled in the art, blood coagulation factor IX and / or activated blood coagulation factor IX , And improved blood coagulation factor VIII-like coagulation activity against antibodies obtained by screening using both blood coagulation factor X binding to target antigen and blood coagulation factor VIII-like coagulation activity as indicators IgG antibody prepared by amino acid substitution.
- ECL As a result of evaluating the binding of MabD1 to D3 to the extracellular matrix by ECL, it was found that the degree of binding of these antibodies to the extracellular matrix varies depending on the antibody.
- solubility is important for preparing a protein pharmaceutical solution, and it is desirable that the solubility of the protein pharmaceutical is high.
- the PEG solution monomer peak area and buffer solution monomer area were calculated for each antibody.
- the solubility of each antibody in the presence of PEG was calculated by the following calculation method.
- Antibody solution concentration (mg / mL) x PEG solution monomer peak area / buffer solution monomer area
- FIG. 9 is a plot of the extracellular matrix binding on the horizontal axis and the solubility in the presence of PEG plotted on the vertical axis. It was found that extracellular matrix binding and solubility were highly correlated, with higher extracellular matrix binding having lower solubility. That is, it was shown that an antibody with high solubility can be obtained by screening an antibody with low extracellular matrix binding.
- Example 7 Improvement of solubility of MabD31 by reducing extracellular matrix binding MabD31 was prepared in the same manner as in Example 1 by methods known to those skilled in the art, and blood coagulation factor IX and / or activated blood coagulation factor IX , And improved blood coagulation factor VIII-like coagulation activity against antibodies obtained by screening using both blood coagulation factor X binding to target antigen and blood coagulation factor VIII-like coagulation activity as indicators It is confirmed that the IgG antibody produced by amino acid substitution is low in solubility. In Example 6, it was shown that an antibody with high solubility can be obtained by screening an antibody with low extracellular matrix binding. Therefore, the surface of the antibody variable region of MabD31 is present on MabD31.
- MabD31 and MabD32 Various modified MabD31 antibodies with amino acid substitutions introduced into the exposed residues were prepared and screened for binding to the extracellular matrix.
- MabD32 with reduced extracellular matrix binding was created by introducing amino acid substitutions into the variable region of MabD31.
- MabD31 and MabD32 solubility was measured under 20 mM Histidine, 150 mM NaCl, pH 6.0 conditions.
- MabD31 solubility was 5.9 mg / mL, while MabD32 solubility was 150 mg / mL or higher. there were.
- the extracellular matrix binding and solubility of MabD31 and MabD32 are shown in FIG.
- solubility of the antibody can be improved by reducing extracellular matrix binding.
- the present invention provides a method for improving the physical properties of a polypeptide or a method for producing a polypeptide with improved physical properties by reducing the binding of a polypeptide having a certain target function to an extracellular matrix.
- improvement of physical properties of the polypeptide can improve plasma kinetics, reduce immunogenicity, or improve solubility, and can provide a polypeptide suitable for use as a pharmaceutical product. It is also possible to evaluate the physical properties of the test polypeptide by comparing the binding activity of the polypeptide whose physical properties are known in advance to the extracellular matrix and the binding activity of the test polypeptide to the extracellular matrix. .
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Abstract
Description
〔1〕 細胞外マトリックスへの結合活性を低減させることによる、ポリペプチドの物性を改善させる方法、
〔2〕 物性の改善が、血漿中動態の改善、免疫原性の低減および溶解度の改善から選ばれる少なくとも1つである、〔1〕に記載の方法、
〔3〕 細胞外マトリックスへの結合活性の低減が、ポリペプチドの少なくとも1つのアミノ酸残基を改変することによって細胞外マトリックスへの結合を低減させる、〔1〕または〔2〕に記載の方法、
〔4〕 細胞外マトリックスへの結合活性が電気化学発光法により測定される、〔1〕~〔3〕のいずれかに記載の方法、
〔5〕 ポリペプチドが、抗原結合活性を有するポリペプチドである、〔1〕~〔4〕のいずれかに記載の方法、
〔6〕 抗原結合活性を有するポリペプチドが抗体である、〔5〕に記載の方法、
〔7〕 物性の改善されたポリペプチドの製造方法であって、細胞外マトリックスへの結合活性が低減されるようにポリペプチドの少なくとも1つのアミノ酸残基を改変する工程を含む製造方法、
〔8〕 物性の改善されたポリペプチドの製造方法であって、該製造方法は、
(a) ポリペプチドの少なくとも1つのアミノ酸残基を改変する工程、
(b) 前記工程(a)で改変されたポリペプチドの細胞外マトリックスへの結合活性を測定する工程、および、
(c) 細胞外マトリックスへの結合活性が改変前と比較して低減されたポリペプチドを選択する工程、
を含む製造方法、
〔9〕 さらに、
(d) 〔8〕の工程(c)で選択されたポリペプチドをコードする遺伝子を得る工程、および、
(e) 前記工程(d)で得られた遺伝子を用いてポリペプチドを製造する工程、
を含む、〔8〕に記載の製造方法、
〔10〕 さらに、
(f) 〔9〕の工程(e)で得られたポリペプチドの少なくとも1つのアミノ酸残基を改変して、前記工程(b)~(e)を繰り返し実施する工程、
を含む、〔9〕に記載の製造方法、
〔11〕 あらかじめ物性のわかっているポリペプチドを基準ポリペプチドとして、基準ポリペプチドより物性の優れたポリペプチドを製造する方法であって、該製造方法は、
(a) 基準ポリペプチドおよび、被検ポリペプチド又は被検ポリペプチドライブラリーを細胞外マトリックスに接触させる工程、
(b) 前記工程(a)で接触させたポリペプチドの細胞外マトリックスへの結合活性を測定する工程、
(c) 前記工程(b)で測定されたポリペプチドの中から基準ポリペプチドより細胞外マトリックスへの結合活性が低いポリペプチドを選択する工程、
(d) 前記工程(c)で選択されたポリペプチドをコードする遺伝子を得る工程、および、
(e) 前記工程(d)で得られた遺伝子を用いてポリペプチドを製造する工程、
を含む製造方法、
〔12〕 物性の改善が血漿中動態の改善、免疫原性の低減および溶解度の改善から選ばれる少なくとも1つである、〔7〕~〔11〕に記載の製造方法、
〔13〕 細胞外マトリックスへの結合活性が電気化学発光法により測定される、〔7〕~〔12〕のいずれかに記載の製造方法、
〔14〕 ポリペプチドが、抗原結合活性を有するポリペプチドである、〔7〕~〔13〕のいずれかに記載の製造方法、
〔15〕 抗原結合活性を有するポリペプチドが抗体である、〔14〕に記載の製造方法、
〔16〕 〔7〕~〔15〕のいずれかに記載の製造方法によって製造されたポリペプチド、
〔17〕 あらかじめ物性のわかっているポリペプチドを基準ポリペプチドとして、基準ポリペプチドより物性の優れたポリペプチドのスクリーニング方法であって、該方法は、
(a) 基準ポリペプチドおよび、被検ポリペプチド又は被検ポリペプチドライブラリーを細胞外マトリックスに接触させる工程、
(b) 前記工程(a)で接触させたポリペプチドの細胞外マトリックスへの結合活性を測定する工程、および、
(c) 前記工程(b)で測定されたポリペプチドの中から基準ポリペプチドより細胞外マトリックスへの結合活性が低いポリペプチドを選択する工程、
を含む、方法、
〔18〕 物性の改善が血漿中動態の改善、免疫原性の低減および溶解度の改善から選ばれる少なくとも1つである、〔17〕に記載のスクリーニング方法、
〔19〕 細胞外マトリックスへの結合活性が電気化学発光法により測定される、〔17〕または〔18〕に記載のスクリーニング方法、
〔20〕 ポリペプチドが、抗原結合活性を有するポリペプチドである、〔17〕~〔19〕のいずれかに記載のスクリーニング方法、
〔21〕 抗原結合活性を有するポリペプチドが抗体である、〔20〕に記載のスクリーニング方法、
〔22〕 被検ポリペプチドの細胞外マトリックスへの結合活性を測定し、あらかじめ物性がわかっている他のポリペプチドの細胞外マトリックスへの結合活性と比較することにより、被検ポリペプチドの物性を評価する方法、
〔23〕 物性がポリペプチドの血漿中動態、免疫原性および溶解度から選ばれる少なくとも1つである、〔22〕に記載の方法、
〔24〕 細胞外マトリックスへの結合活性の測定が電気化学発光法による測定である、〔22〕または〔23〕に記載の方法、
に関する。
(a) ポリペプチドの少なくとも1つのアミノ酸残基を改変する工程、
(b) 前記工程(a)で改変されたポリペプチドの細胞外マトリックスへの結合活性を測定する工程、および、
(c) 細胞外マトリックスへの結合活性が改変前と比較して低減されたポリペプチドを選択する工程。
なお、(a)と(b)の工程は2回以上繰り返されてもよい。(a)と(b)の工程が繰り返される回数は特に限定されないが、通常10回以内である。
(d) 前記工程(c)で選択されたポリペプチドをコードする遺伝子を得る工程、および、
(e) 前記工程(d)で得られた遺伝子を用いてポリペプチドを製造する工程。
(f) 前記工程(e)で得られたポリペプチドの少なくとも1つのアミノ酸残基を改変して、前記工程(b)-(e)を繰り返し実施する工程
なお、工程(f)において繰り返される(b)-(e)の工程の回数は、特に限定されないが、通常10回以内である。
(a) 基準ポリペプチドおよび、被検ポリペプチド又は被検ポリペプチドライブラリーを細胞外マトリックスに接触させる工程、
(b) 前記工程(a)で接触させたポリペプチドの細胞外マトリックスへの結合活性を測定する工程、
(c) 前記工程(b)で測定されたポリペプチドの中から基準ポリペプチドより細胞外マトリックスへの結合活性が低いポリペプチドを選択する工程、
(d) 前記工程(c)で選択されたポリペプチドをコードする遺伝子を得る工程、および、
(e) 前記工程(d)で得られた遺伝子を用いてポリペプチドを製造する工程。
なお、(a)~(c)の工程は2回以上繰り返されてもよい。(a)~(c)の工程が繰り返される回数は特に限定されないが、通常10回以内である。
(a) 基準ポリペプチドおよび、被検ポリペプチド又は被検ポリペプチドライブラリーを細胞外マトリックスに接触させる工程、
(b) 前記工程(a)で接触させたポリペプチドの細胞外マトリックスへの結合活性を測定する工程、および、
(c) 前記工程(b)で測定されたポリペプチドの中から基準ポリペプチドより細胞外マトリックスへの結合活性が低いポリペプチドを選択する工程。
なお、(a)~(c)の工程は2回以上繰り返されてもよい。(a)~(c)の工程が繰り返される回数は特に限定されないが、通常10回以内である。
また本発明は、本発明のポリぺプチド、本発明の製造方法により製造されたポリペプチドまたは上記本発明の医薬組成物を対象へ投与することによる、血漿中動態を改善する方法、または免疫原性を低減する方法に関する。投与は、in vivoあるいはin vitroのどちらで行われてもよい。投与対象としては、例えば非ヒト動物(マウス、サルなど)、あるいはヒトなどを挙げることができる。
MabA1、A2、A3は血液凝固第IX因子および/または活性化血液凝固第IX因子、並びに、血液凝固第X因子を標的抗原として特異的な結合を示し、血液凝固第VIII因子様凝固活性を発揮する二重特異性抗体であり、これらの抗体は、WO2005/035756およびWO2006/109592に記載の方法を用いて、当業者公知の抗体取得技術から、当該標的抗原への結合および凝固活性を指標にしたスクリーニングを行い、さらに血液凝固第VIII因子様凝固活性を向上させるアミノ酸改変を加えることによって取得されたIgG抗体である。
MabA1、A2、A3を正常マウス(C57BL/6J、日本チャールズリバー)に1 mg/kgで単回投与し、適時採血を行った。採取した血液は直ちに4℃、15,000 rpmで15分間遠心分離し、血漿を得た。分離した血漿は、測定を実施するまで-20℃以下に設定された冷凍庫に保存した。
MabB1は、実施例1と同様の方法で、当業者公知の方法により、血液凝固第IX因子および/または活性化血液凝固第IX因子、並びに、血液凝固第X因子双方の標的抗原への結合および血液凝固第VIII因子様凝固活性を指標にしたスクリーニングを行うことによって取得した抗体に対して、血液凝固第VIII因子様凝固活性を向上させるアミノ酸置換を行い作製したIgG抗体である。MabB1は血漿中半減期が非常に短く、MabB1を医薬品として開発するうえで短い血漿中半減期は投与量の増大と投与間隔の短縮につながるため大きな課題である。MabB1の細胞外マトリックスに対する結合を評価した結果、MabB1は細胞外マトリックスに強く結合することが確認され、血漿中半減期が短いのは細胞外マトリックスへの非特異的な結合が原因と考えられた。そこで、細胞外マトリックスへの非特異的な結合を低減させることによりMabB1の血漿中薬物動態を改善することを検討した。
MabC9は、実施例1と同様の方法で、当業者公知の方法により、血液凝固第IX因子および/または活性化血液凝固第IX因子、並びに、血液凝固第X因子双方の標的抗原への結合および血液凝固第VIII因子様凝固活性を指標にしたスクリーニングを行うことによって取得した抗体に対して、血液凝固第VIII因子様凝固活性を向上させるアミノ酸置換を行い作製したIgG抗体である。MabC9は血漿中半減期が非常に短いことが分っていたため、MabC9の血漿中薬物動態を改善することを検討した。その結果、MabC9は細胞外マトリックスに強く結合することが確認され、血漿中半減期が短いのは細胞外マトリックスへの非特異的な結合が原因と考えられた。
MabC1~C6は、実施例1と同様の方法で、当業者公知の方法により、血液凝固第IX因子および/または活性化血液凝固第IX因子、並びに、血液凝固第X因子双方の標的抗原への結合および血液凝固第VIII因子様凝固活性を指標にしたスクリーニングを行うことによって取得した抗体に対して、血液凝固第VIII因子様凝固活性を向上させるアミノ酸置換を行い作製したIgG抗体である。MabC1~C6の細胞外マトリックスへの結合をECLにより評価した結果、これらの抗体の細胞外マトリックスへの結合の程度は抗体によって異なることが分った。細胞外マトリックスに結合する抗体は、標的抗原以外に対して非特異的な結合を示すため、抗原提示細胞に対しても結合する可能性がある。そのため、細胞外マトリックスに結合する抗体は、抗原提示細胞に取り込まれやすく、それにより抗原提示されやすくなると考えられ、免疫原性が高くなる可能性が考えられた。免疫原性はタンパク質医薬品の重要な課題であり、タンパク質医薬品の免疫原性は低いことが望ましい。
実施例2で作製されたMabB2は、マウスに投与すると抗MabB2マウス抗体が短期間に産生されることから免疫原性が高いことが確認されている。実施例4において、細胞外マトリックス結合能が低い抗体ほど免疫原性が低く、細胞外マトリックス結合能を低減させることで抗体の免疫原性を低減することが可能であることが示唆されたことから、MabB2およびMabB3のマウスでの血漿中滞留性評価において、マウス血漿中抗MabB2抗体および抗MabB3抗体をECL免疫測定法にて検出した。まず抗体をEZ-LinkTM Sulfo-NFS-Biotinylation Kit (PIERCE社製) を用いてBiotin化、及びSulfo-Tag NHS Ester 150 nmol (MSD社製)を用いてsTag化した。マウス血漿測定試料、Biotin化抗体及びsTag化抗体を混和後、4℃で一晩静置した。この試料をMULTI-ARRAY(R) PR SA Plate(MSD社製) に分注し、室温で2時間振盪した。その後、MSD Read Buffer T (4x) (MSD社製) を添加し、SECTOR PR(R) 400にて発光量を測定した。横軸に経過時間(日)、縦軸に抗体価(ECL signal)をプロットしたものを図8に示した。細胞外マトリックス結合能を低減させたMabB3はMabB2と比較して抗体価が低く、免疫原性が低減されていることが確認された。
MabD1~D30は、実施例1と同様の方法で、当業者公知の方法により、血液凝固第IX因子および/または活性化血液凝固第IX因子、並びに、血液凝固第X因子双方の標的抗原への結合および血液凝固第VIII因子様凝固活性を指標にしたスクリーニングを行うことによって取得した抗体に対して、血液凝固第VIII因子様凝固活性を向上させるアミノ酸置換を行い作製したIgG抗体である。MabD1~D3の細胞外マトリックスへの結合をECLにより評価した結果、これらの抗体の細胞外マトリックスへの結合の程度は抗体によって異なることが分かった。タンパク質医薬品の溶液製剤を調製するためには、十分にそのタンパク質が高い溶解度を有している必要がある。タンパク質同士が自己会合化(凝集)しやすい場合は一般に溶解度が低いと考えられる。細胞外マトリックス結合する抗体は非特異的にタンパク質に結合しやすい性質を有していると考えられることから、溶解度も低くなる可能性が考えられた。溶解度はタンパク質医薬品の溶液製剤化に重要であり、タンパク質医薬品の溶解度は高いことが望ましい。
移動相:50 mM sodium phosphate, 500 mM NaCl, pH7.0
流速:1.0 mL/min
検出波長:220 nm
MabD31は、実施例1と同様の方法で、当業者公知の方法により、血液凝固第IX因子および/または活性化血液凝固第IX因子、並びに、血液凝固第X因子双方の標的抗原への結合および血液凝固第VIII因子様凝固活性を指標にしたスクリーニングを行うことによって取得した抗体に対して、血液凝固第VIII因子様凝固活性を向上させるアミノ酸置換を行い作製したIgG抗体であり、溶解度が低いことが確認されている。実施例6において、細胞外マトリックス結合が低い抗体をスクリーニングすることで溶解度が高い抗体を取得することが出来ることが示されたことから、MabD31に対して、MabD31の抗体可変領域に存在する表面に露出している残基にアミノ酸置換を導入した各種改変MabD31抗体を作製し、細胞外マトリックスに対する結合のスクリーニングを実施した。MabD31の可変領域にアミノ酸置換を導入することで細胞外マトリックス結合を低減させたMabD32を作製した。MabD31とMabD32の溶解度を20 mM Histidine, 150 mM NaCl, pH6.0条件下において測定した結果、MabD31の溶解度は5.9 mg/mLであったのに対して、MabD32の溶解度は150 mg/mL以上であった。MabD31とMabD32の細胞外マトリックス結合と溶解度を図10に示した。
Claims (24)
- 細胞外マトリックスへの結合活性を低減させることによる、ポリペプチドの物性を改善させる方法。
- 物性の改善が、血漿中動態の改善、免疫原性の低減および溶解度の改善から選ばれる少なくとも1つである、請求項1に記載の方法。
- 細胞外マトリックスへの結合活性の低減が、ポリペプチドの少なくとも1つのアミノ酸残基を改変することによって細胞外マトリックスへの結合を低減させる、請求項1または2に記載の方法。
- 細胞外マトリックスへの結合活性が電気化学発光法により測定される、請求項1~3のいずれかに記載の方法。
- ポリペプチドが、抗原結合活性を有するポリペプチドである、請求項1~4のいずれかに記載の方法。
- 抗原結合活性を有するポリペプチドが抗体である、請求項5に記載の方法。
- 物性の改善されたポリペプチドの製造方法であって、細胞外マトリックスへの結合活性が低減されるようにポリペプチドの少なくとも1つのアミノ酸残基を改変する工程を含む製造方法。
- 物性の改善されたポリペプチドの製造方法であって、該製造方法は、
(a) ポリペプチドの少なくとも1つのアミノ酸残基を改変する工程、
(b) 前記工程(a)で改変されたポリペプチドの細胞外マトリックスへの結合活性を測定する工程、および、
(c) 細胞外マトリックスへの結合活性が改変前と比較して低減されたポリペプチドを選択する工程、
を含む製造方法。 - さらに、
(d) 請求項8の工程(c)で選択されたポリペプチドをコードする遺伝子を得る工程、および、
(e) 前記工程(d)で得られた遺伝子を用いてポリペプチドを製造する工程、
を含む、請求項8に記載の製造方法。 - さらに、
(f) 請求項9の工程(e)で得られたポリペプチドの少なくとも1つのアミノ酸残基を改変して、前記工程(b)~(e)を繰り返し実施する工程、
を含む、請求項9に記載の製造方法。 - あらかじめ物性のわかっているポリペプチドを基準ポリペプチドとして、基準ポリペプチドより物性の優れたポリペプチドを製造する方法であって、該製造方法は、
(a) 基準ポリペプチドおよび、被検ポリペプチド又は被検ポリペプチドライブラリーを細胞外マトリックスに接触させる工程、
(b) 前記工程(a)で接触させたポリペプチドの細胞外マトリックスへの結合活性を測定する工程、
(c) 前記工程(b)で測定されたポリペプチドの中から基準ポリペプチドより細胞外マトリックスへの結合活性が低いポリペプチドを選択する工程、
(d) 前記工程(c)で選択されたポリペプチドをコードする遺伝子を得る工程、および、
(e) 前記工程(d)で得られた遺伝子を用いてポリペプチドを製造する工程
を含む製造方法。 - 物性の改善が血漿中動態の改善、免疫原性の低減および溶解度の改善から選ばれる少なくとも1つである、請求項7~11に記載の製造方法。
- 細胞外マトリックスへの結合活性が電気化学発光法により測定される、請求項7~12のいずれかに記載の製造方法。
- ポリペプチドが、抗原結合活性を有するポリペプチドである、請求項7~13のいずれかに記載の製造方法。
- 抗原結合活性を有するポリペプチドが抗体である、請求項14に記載の製造方法。
- 請求項7~15のいずれかに記載の製造方法によって製造されたポリペプチド。
- あらかじめ物性のわかっているポリペプチドを基準ポリペプチドとして、基準ポリペプチドより物性の優れたポリペプチドのスクリーニング方法であって、該方法は、
(a) 基準ポリペプチドおよび、被検ポリペプチド又は被検ポリペプチドライブラリーを細胞外マトリックスに接触させる工程、
(b) 前記工程(a)で接触させたポリペプチドの細胞外マトリックスへの結合活性を測定する工程、および、
(c) 前記工程(b)で測定されたポリペプチドの中から基準ポリペプチドより細胞外マトリックスへの結合活性が低いポリペプチドを選択する工程
を含む、方法。 - 物性の改善が血漿中動態の改善、免疫原性の低減および溶解度の改善から選ばれる少なくとも1つである、請求項17に記載のスクリーニング方法。
- 細胞外マトリックスへの結合活性が電気化学発光法により測定される、請求項17または18に記載のスクリーニング方法。
- ポリペプチドが、抗原結合活性を有するポリペプチドである、請求項17~19のいずれかに記載のスクリーニング方法。
- 抗原結合活性を有するポリペプチドが抗体である、請求項20に記載のスクリーニング方法。
- 被検ポリペプチドの細胞外マトリックスへの結合活性を測定し、あらかじめ物性がわかっている他のポリペプチドの細胞外マトリックスへの結合活性と比較することにより、被検ポリペプチドの物性を評価する方法。
- 物性がポリペプチドの血漿中動態、免疫原性および溶解度から選ばれる少なくとも1つである、請求項22に記載の方法。
- 細胞外マトリックスへの結合活性の測定が電気化学発光法による測定である、請求項22または23に記載の方法。
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Citations (16)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP0125023A1 (en) | 1983-04-08 | 1984-11-14 | Genentech, Inc. | Recombinant immunoglobulin preparations, methods for their preparation, DNA sequences, expression vectors and recombinant host cells therefor |
WO1995001937A1 (fr) | 1993-07-09 | 1995-01-19 | Association Gradient | Procede de traitement de residus de combustion et installation de mise en ×uvre dudit procede |
WO1996002576A1 (fr) | 1994-07-13 | 1996-02-01 | Chugai Seiyaku Kabushiki Kaisha | Anticorps humain reconstitue contre l'interleukine-8 humaine |
JPH11507726A (ja) * | 1995-06-07 | 1999-07-06 | イゲン,インコーポレイテッド | 電気化学発光酵素イムノアッセイ |
JP2000028609A (ja) * | 1998-06-25 | 2000-01-28 | Roche Diagnostics Gmbh | 化学発光および電気化学発光検出法における誘導体化試薬の使用 |
WO2002020565A2 (en) | 2000-09-08 | 2002-03-14 | Universität Zürich | Collections of repeat proteins comprising repeat modules |
WO2002032925A2 (en) | 2000-10-16 | 2002-04-25 | Phylos, Inc. | Protein scaffolds for antibody mimics and other binding proteins |
WO2003002609A2 (en) | 2001-06-28 | 2003-01-09 | Domantis Limited | Dual-specific ligand and its use |
JP2003501089A (ja) * | 1999-06-08 | 2003-01-14 | リジェネロン・ファーマシューティカルズ・インコーポレイテッド | 改善された薬物動態特性を有する改変キメラポリペプチド |
WO2004044011A2 (en) | 2002-11-06 | 2004-05-27 | Avidia Research Institute | Combinatorial libraries of monomer domains |
WO2004058821A2 (en) | 2002-12-27 | 2004-07-15 | Domantis Limited | Dual specific single domain antibodies specific for a ligand and for the receptor of the ligand |
WO2005035756A1 (ja) | 2003-10-10 | 2005-04-21 | Chugai Seiyaku Kabushiki Kaisha | 機能蛋白質を代替する二種特異性抗体 |
WO2005037989A2 (en) | 2001-01-17 | 2005-04-28 | Trubion Pharmaceuticals, Inc. | Binding domain-immunoglobulin fusion proteins |
WO2005040229A2 (en) | 2003-10-24 | 2005-05-06 | Avidia, Inc. | Ldl receptor class a and egf domain monomers and multimers |
WO2006109592A1 (ja) | 2005-04-08 | 2006-10-19 | Chugai Seiyaku Kabushiki Kaisha | 血液凝固第viii因子の機能代替抗体 |
WO2009128931A1 (en) | 2008-04-18 | 2009-10-22 | Abbott Laboratories | In vitro estimation of in vivo half-life binding proteins |
Family Cites Families (9)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6316607B1 (en) * | 1986-04-30 | 2001-11-13 | Igen International, Inc. | Electrochemiluminescent assays |
US6075007A (en) * | 1997-07-17 | 2000-06-13 | Regeneron Pharmaceuticals, Inc. | Modified noggin polypeptide and compositions |
US20040266688A1 (en) * | 2003-05-14 | 2004-12-30 | Nayak Nihar R | Methods for modulating endometrium |
CA2554089C (en) * | 2004-02-09 | 2013-10-22 | Human Genome Sciences, Inc. | Albumin fusion proteins |
DK1809578T3 (da) * | 2004-10-19 | 2010-08-30 | Green Gas Company As | Fremgangsmåde og anlæg til fremstilling af biogas ved hjælp af anaerob hydrolyse |
CA2595169A1 (en) * | 2005-01-12 | 2006-07-20 | Xencor, Inc. | Antibodies and fc fusion proteins with altered immunogenicity |
JP5624276B2 (ja) * | 2006-03-31 | 2014-11-12 | 中外製薬株式会社 | 抗体の血中動態を制御する方法 |
TWI440469B (zh) * | 2008-09-26 | 2014-06-11 | Chugai Pharmaceutical Co Ltd | Improved antibody molecules |
AR075908A1 (es) * | 2009-03-19 | 2011-05-04 | Chugai Pharmaceutical Co Ltd | Formulacion farmaceutica que contiene moleculas de anticuerpo mejoradas, polipeptidos. metodo para estabilizar solucion, anticuerpo. |
-
2012
- 2012-01-06 WO PCT/JP2012/050129 patent/WO2012093704A1/ja active Application Filing
- 2012-01-06 US US13/978,232 patent/US20140080153A1/en not_active Abandoned
- 2012-01-06 EP EP19155042.5A patent/EP3539991A1/en active Pending
- 2012-01-06 JP JP2012551878A patent/JP6043629B2/ja active Active
- 2012-01-06 EP EP12731990.3A patent/EP2662385A4/en not_active Withdrawn
-
2016
- 2016-08-25 JP JP2016164225A patent/JP6469052B2/ja active Active
Patent Citations (16)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP0125023A1 (en) | 1983-04-08 | 1984-11-14 | Genentech, Inc. | Recombinant immunoglobulin preparations, methods for their preparation, DNA sequences, expression vectors and recombinant host cells therefor |
WO1995001937A1 (fr) | 1993-07-09 | 1995-01-19 | Association Gradient | Procede de traitement de residus de combustion et installation de mise en ×uvre dudit procede |
WO1996002576A1 (fr) | 1994-07-13 | 1996-02-01 | Chugai Seiyaku Kabushiki Kaisha | Anticorps humain reconstitue contre l'interleukine-8 humaine |
JPH11507726A (ja) * | 1995-06-07 | 1999-07-06 | イゲン,インコーポレイテッド | 電気化学発光酵素イムノアッセイ |
JP2000028609A (ja) * | 1998-06-25 | 2000-01-28 | Roche Diagnostics Gmbh | 化学発光および電気化学発光検出法における誘導体化試薬の使用 |
JP2003501089A (ja) * | 1999-06-08 | 2003-01-14 | リジェネロン・ファーマシューティカルズ・インコーポレイテッド | 改善された薬物動態特性を有する改変キメラポリペプチド |
WO2002020565A2 (en) | 2000-09-08 | 2002-03-14 | Universität Zürich | Collections of repeat proteins comprising repeat modules |
WO2002032925A2 (en) | 2000-10-16 | 2002-04-25 | Phylos, Inc. | Protein scaffolds for antibody mimics and other binding proteins |
WO2005037989A2 (en) | 2001-01-17 | 2005-04-28 | Trubion Pharmaceuticals, Inc. | Binding domain-immunoglobulin fusion proteins |
WO2003002609A2 (en) | 2001-06-28 | 2003-01-09 | Domantis Limited | Dual-specific ligand and its use |
WO2004044011A2 (en) | 2002-11-06 | 2004-05-27 | Avidia Research Institute | Combinatorial libraries of monomer domains |
WO2004058821A2 (en) | 2002-12-27 | 2004-07-15 | Domantis Limited | Dual specific single domain antibodies specific for a ligand and for the receptor of the ligand |
WO2005035756A1 (ja) | 2003-10-10 | 2005-04-21 | Chugai Seiyaku Kabushiki Kaisha | 機能蛋白質を代替する二種特異性抗体 |
WO2005040229A2 (en) | 2003-10-24 | 2005-05-06 | Avidia, Inc. | Ldl receptor class a and egf domain monomers and multimers |
WO2006109592A1 (ja) | 2005-04-08 | 2006-10-19 | Chugai Seiyaku Kabushiki Kaisha | 血液凝固第viii因子の機能代替抗体 |
WO2009128931A1 (en) | 2008-04-18 | 2009-10-22 | Abbott Laboratories | In vitro estimation of in vivo half-life binding proteins |
Non-Patent Citations (20)
Title |
---|
"Current protocols in Molecular Biology", 1987, JOHN WILEY & SONS |
"Strategies for Protein Purification and Characterization: A Laboratory Course Manual", 1996, COLD SPRING HARBOR LABORATORY PRESS |
BAKER MP; CARR FJ, CURR DRUG SAF., vol. 5, no. 4, 2010, pages 308 - 13 |
CHOTHIA ET AL., NATURE, vol. 342, 1989, pages 877 |
COX KM ET AL., NAT. BIOTECHNOL., vol. 24, no. 12, December 2006 (2006-12-01), pages 1591 - 1597 |
CURRENT OPINION IN BIOTECHNOLOGY, vol. 17, 2006, pages 653 - 658 |
CURRENT OPINION IN BIOTECHNOLOGY, vol. 18, 2007, pages 1 - 10 |
CURRENT OPINION IN STRUCTURAL BIOLOGY, vol. 7, 1997, pages 463 - 469 |
EBERT ET AL., BIO/TECHNOLOGY, vol. 12, 1994, pages 699 - 702 |
GUIOTTO ET AL., BIOORG. MED. CHEM., vol. 12, 2004, pages 5031 - 5037 |
GUIOTTO, A. ET AL., BIOORG. MED. CHEM., vol. 12, 2004, pages 5031 - 5037 |
KABAT ET AL.: "Sequence of Proteins of Immunological Interest", 1987, NATIONAL INSTITUTE OF HEALTH |
KUNKEL, PROC. NATL. ACAD. SCI. USA, vol. 82, 1985, pages 488 |
MA ET AL., EUR. J. IMMUNOL., vol. 24, 1994, pages 131 - 138 |
MOL CELL BIOL., vol. 8, 1988, pages 466 - 472 |
NAT BIOTECHNOL., vol. 23, no. 9, September 2005 (2005-09-01), pages 1126 - 36 |
PROC. NATL. ACAD. SCI., vol. 99, no. 17, 2002, pages 11393 - 11398 |
PROTEIN SCIENCE, vol. 15, 2006, pages 14 - 27 |
See also references of EP2662385A4 |
VICKI GLASER, SPECTRUM BIOTECHNOLOGY APPLICATIONS, 1993 |
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Also Published As
Publication number | Publication date |
---|---|
EP3539991A1 (en) | 2019-09-18 |
EP2662385A4 (en) | 2015-11-11 |
JP6043629B2 (ja) | 2016-12-14 |
EP2662385A1 (en) | 2013-11-13 |
JPWO2012093704A1 (ja) | 2014-06-09 |
JP6469052B2 (ja) | 2019-02-13 |
JP2017071595A (ja) | 2017-04-13 |
US20140080153A1 (en) | 2014-03-20 |
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