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WO2012093149A3 - Novel cell wall deconstruction enzymes and uses thereof - Google Patents

Novel cell wall deconstruction enzymes and uses thereof Download PDF

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Publication number
WO2012093149A3
WO2012093149A3 PCT/EP2012/050130 EP2012050130W WO2012093149A3 WO 2012093149 A3 WO2012093149 A3 WO 2012093149A3 EP 2012050130 W EP2012050130 W EP 2012050130W WO 2012093149 A3 WO2012093149 A3 WO 2012093149A3
Authority
WO
WIPO (PCT)
Prior art keywords
seq
nos
set forth
nucleic acids
polypeptide
Prior art date
Application number
PCT/EP2012/050130
Other languages
French (fr)
Other versions
WO2012093149A2 (en
Inventor
Adrian Tsang
Justin Powlowski
Gregory Butler
Reginald Storms
Original Assignee
Dsm Ip Assets B.V.
Valorbec Societe En Commandite
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Dsm Ip Assets B.V., Valorbec Societe En Commandite filed Critical Dsm Ip Assets B.V.
Publication of WO2012093149A2 publication Critical patent/WO2012093149A2/en
Publication of WO2012093149A3 publication Critical patent/WO2012093149A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/02—Monosaccharides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/06—Ethanol, i.e. non-beverage
    • C12P7/08—Ethanol, i.e. non-beverage produced as by-product or from waste or cellulosic material substrate
    • C12P7/10—Ethanol, i.e. non-beverage produced as by-product or from waste or cellulosic material substrate substrate containing cellulosic material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y114/00—Oxidoreductases acting on paired donors, with incorporation or reduction of molecular oxygen (1.14)
    • C12Y114/99—Miscellaneous (1.14.99)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01004—Cellulase (3.2.1.4), i.e. endo-1,4-beta-glucanase
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/10—Biofuels, e.g. bio-diesel

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

The present invention relates to a novel process for degrading biomass or pretreated biomass to sugars wherein an enzyme is used comprising a polypeptide having a) a polypeptide sequence as set forth in any one of SEQ ID Nos: 3, 6, 9, 12, 15, 18, 21, 24, 27, 30, 33, 36, 39, 42, 45, 48, 51, 54, 57, 60, 63, 66 and 69; b) a polypeptide that is at least 80%, preferably at least 85%, more preferably at least 90%, even more preferably at least 95%, 96%, 97%, 98% or 99% homologous to the any one of SEQ ID Nos: 3, 6, 9, 12, 15, 18, 21, 24, 27, 30, 33, 36, 39, 42, 45, 48, 51, 54, 57, 60, 63, 66 and 69; c) a polypeptide sequence encoded by nucleic acids sequence as set forth in any one of SEQ ID NOs: 1, 2, 4, 5, 7, 8, 10, 11, 13, 14, 16, 17, 19, 20, 22, 23, 25, 26, 28, 29, 31, 32, 34, 35, 37, 38, 40, 41, 43, 44, 46, 47, 49, 50, 52, 53, 55, 56, 58, 59, 61, 62, 64, 65, 67 and 68, or nucleic acids that are at least 80%, preferably at least 85%, more preferably at least 90%, even more preferably at least 95%, 96%, 97%, 98% or 99% homologous to any one of SEQ ID NOs: 1, 2, 4, 5, 7, 8, 10, 11, 13, 14, 16, 17, 19, 20, 22, 23, 25, 26, 28, 29, 31, 32, 34, 35, 37, 38, 40, 41, 43, 44, 46, 47, 49, 50, 52, 53, 55, 56, 58, 59, 61, 62, 64, 65, 67 and 68; d) a polypeptide sequence encoded by a nucleic acids sequence hybridizing under stringent conditions to the polynucleotide as set forth in any one of SEQ ID NOs: 1, 2, 4, 5, 7, 8, 10, 11, 13, 14, 16, 17, 19, 20, 22, 23, 25, 26, 28, 29, 31, 32, 34, 35, 37, 38, 40, 41, 43, 44, 46, 47, 49, 50, 52, 53, 55, 56, 58, 59, 61, 62, 64, 65, 67 and 68; or e) a polypeptide sequence encoded by a nucleic acids sequence hybridizing under stringent conditions to the reverse complement of a polynucleotide as set forth in any one of SEQ ID NOs: 1, 2, 4, 5, 7, 8, 10, 11, 13, 14, 16, 17, 19, 20, 22, 23, 25, 26, 28, 29, 31, 32, 34, 35, 37, 38, 40, 41, 43, 44, 46, 47, 49, 50, 52, 53, 55, 56, 58, 59, 61, 62, 64, 65, 67 and 68.
PCT/EP2012/050130 2011-01-06 2012-01-05 Novel cell wall deconstruction enzymes and uses thereof WO2012093149A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201161430533P 2011-01-06 2011-01-06
US61/430,533 2011-01-06

Publications (2)

Publication Number Publication Date
WO2012093149A2 WO2012093149A2 (en) 2012-07-12
WO2012093149A3 true WO2012093149A3 (en) 2013-08-22

Family

ID=45443127

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2012/050130 WO2012093149A2 (en) 2011-01-06 2012-01-05 Novel cell wall deconstruction enzymes and uses thereof

Country Status (1)

Country Link
WO (1) WO2012093149A2 (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111100889B (en) * 2018-10-26 2021-08-31 中国石油化工股份有限公司 Method for improving lignocellulose enzymolysis efficiency

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2897973A1 (en) * 2012-09-19 2015-07-29 Novozymes A/S Polypeptides having cellulolytic enhancing activity and polynucleotides encoding same
CN104342424B (en) * 2013-07-29 2017-12-01 瑞辰星生物技术（广州）有限公司 For changing and improving fiber oxidation enzymatic compositions and papermaking process and the application of fibre property
US20170166939A1 (en) 2013-11-26 2017-06-15 Novozymes A/S Enzyme Compositions and Uses Thereof
CN115975994A (en) 2014-06-06 2023-04-18 诺维信公司 Enzyme composition and use thereof
BR112017019332A2 (en) 2015-03-12 2018-07-24 Beta Renewables Spa processes for improving a saccharification glucose or xylose yield of a lignocellulosic material and for producing a fermentation product from a lignocellulosic material
WO2016145358A1 (en) 2015-03-12 2016-09-15 Novozymes A/S Enzymatic hydrolysis with hemicellulolytic enzymes
WO2016145363A1 (en) 2015-03-12 2016-09-15 Novozymes A/S Multi-stage enzymatic hydrolysis of lignocellulosic biomass employing an oxidoreductase with an aa9 polypeptide

Citations (2)

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WO2003070940A1 (en) * 2002-02-25 2003-08-28 Oji Paper Co., Ltd. Cellulose digesting enzyme gene and utilization of the gene
JP2009124995A (en) * 2007-11-22 2009-06-11 Oji Paper Co Ltd Lignocellulase gene and its use

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WO2003070940A1 (en) * 2002-02-25 2003-08-28 Oji Paper Co., Ltd. Cellulose digesting enzyme gene and utilization of the gene
JP2009124995A (en) * 2007-11-22 2009-06-11 Oji Paper Co Ltd Lignocellulase gene and its use

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111100889B (en) * 2018-10-26 2021-08-31 中国石油化工股份有限公司 Method for improving lignocellulose enzymolysis efficiency

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Publication number Publication date
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