[go: up one dir, main page]
More Web Proxy on the site http://driver.im/

WO2012057781A1 - Compositions de nettoyage et/ou de traitement comprenant une sérine protéase fongique - Google Patents

Compositions de nettoyage et/ou de traitement comprenant une sérine protéase fongique Download PDF

Info

Publication number
WO2012057781A1
WO2012057781A1 PCT/US2010/054670 US2010054670W WO2012057781A1 WO 2012057781 A1 WO2012057781 A1 WO 2012057781A1 US 2010054670 W US2010054670 W US 2010054670W WO 2012057781 A1 WO2012057781 A1 WO 2012057781A1
Authority
WO
WIPO (PCT)
Prior art keywords
cleaning
treatment composition
fabric
mixtures
group
Prior art date
Application number
PCT/US2010/054670
Other languages
English (en)
Inventor
Neil Joseph Lant
Original Assignee
The Procter & Gamble Company
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by The Procter & Gamble Company filed Critical The Procter & Gamble Company
Priority to PCT/US2010/054670 priority Critical patent/WO2012057781A1/fr
Priority to US13/278,201 priority patent/US20120108488A1/en
Publication of WO2012057781A1 publication Critical patent/WO2012057781A1/fr
Priority to US14/833,516 priority patent/US20150353870A1/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
    • C11D3/16—Organic compounds
    • C11D3/38—Products with no well-defined composition, e.g. natural products
    • C11D3/386—Preparations containing enzymes, e.g. protease or amylase
    • C11D3/38618—Protease or amylase in liquid compositions only
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
    • C11D3/16—Organic compounds
    • C11D3/38—Products with no well-defined composition, e.g. natural products
    • C11D3/386—Preparations containing enzymes, e.g. protease or amylase
    • C11D3/38609—Protease or amylase in solid compositions only

Definitions

  • This invention relates to cleaning and/or treatment products comprising fungal serine proteases as well as methods of making and using such cleaning and/or treatment products.
  • proteases into their products to provide good cleaning of proteinaceous stains (such as blood).
  • proteinaceous stains such as blood
  • current proteases have very low activity levels, for example 10% of their maximum activity, in the typical low wash temperatures of 5°C to 20°C.
  • Applicant has surprisingly recognized that when consumer products are formulated with certain .fungal proteases, 50% to 70% of the enzyme's maximum activity is obtained.
  • cleaning of proteinaceous stains is greatly improved and surprisingly the performance of other cleaning ingredients is enhanced.
  • the performance of proteases other than the aforementioned fungal protease is improved, lipolytic action of lipases is enhanced, amylolytic action of amylases is enhanced, the catalytic bleaching action of bleach catalysts is increased, hueing agent performance is increased, the action of chelants is enhanced and the performance of perfume microcapsules is improved.
  • This invention relates to cleaning and/or treatment products comprising fungal proteases and processes for making and using such products.
  • Such compositions provide improved cleaning and freshness.
  • proteases are wild types or are derived from such wild types, by substitution, insertion and/or deletion of one or more of the parent enzymes' amino acids.
  • cleaning and/or treatment composition includes, unless otherwise indicated, granular or powder-form all-purpose or "heavy-duty” washing agents, especially laundry detergents; liquid, gel or paste- form all-purpose washing agents, especially the so-called heavy-duty liquid types; liquid fine-fabric detergents; hand dishwashing agents or light duty dishwashing agents, especially those of the high-foaming type; machine dishwashing agents, including the various tablet, unit dose liquid tablets /pouches, impregnated non woven sheets, granular, liquid and rinse-aid types for household and institutional use; liquid cleaning and disinfecting agents, including antibacterial hand-wash types, laundry bars, car or carpet shampoos, bathroom cleaners; hair; as well as cleaning auxiliaries such as bleach additives and "stain-stick" or pre-treat types.
  • component or composition levels are in reference to the active level of that component or composition, and are exclusive of impurities, for example, residual solvents or by-products, which may be present in commercially available sources.
  • the enzymes of the present invention are expressed in terms of active protein level and are exclusive of impurities, for example, residual solvents or byproducts, which may be present in commercially available sources.
  • identity in the context of two polypeptide sequences refers to the residues in the two sequences that are the same when aligned for maximum correspondence, as measured using one of the following sequence comparison or analysis algorithms.
  • optimal alignment refers to the alignment giving the highest percent identity score.
  • Percent sequence identity “percent amino acid sequence identity,” with respect to two amino acid sequences, refer to the percentage of residues that are identical in the two sequences when the sequences are optimally aligned. Thus, 80% amino acid sequence identity means that 80% of the amino acids in two optimally aligned polypeptide sequences are identical. Alignment of the two polypeptide sequences may be conducted using the programs or algorithms (e.g., BLAST, ALIGN,
  • the composition comprises a fungal serine protease having at least 56%, 70%, 75%, 80%, 85%, 90%, 95%, 99%, or even complete identity to SEQ ID NO: 1.
  • SEQ ID NO: 1 is the amino acid sequence of a fungal serine protease derived from Trichoderma reesei strain QM9414.
  • the fungal serine protease is the endopeptidase from Trichoderma harzianum strain CAL25577 having the amino acid sequence of SEQ ID NO: 2, which has 81% identity to SEQ ID NO: 1.
  • the fungal serine protease is the trypsin precursor from Pyrenophora tritici-repentis strain Pt-lc-BFP having the amino acid sequence of SEQ ID NO: 3, which has 63% identity to SEQ ID NO: 1.
  • the fungal serine protease is the trypsin-like protease from Trichoderma harzianum strain CECT 2413 having the amino acid sequence of SEQ ID NO: 4, which has 65% identity to SEQ ID NO: 1.
  • the fungal serine proteases can be produced using standard biochemical means. For example, a procedure for the isolation of the fungal serine protease derived from Trichoderma reesei strain QM9414 defined by SEQ ID NO: 1, is found in Example 1 of this specification.
  • the fungal serine protease is a protein engineered variant of one of the four wild- type enzymes defined by SEQ ID NOS: 1-4, having at an amino acid sequence with at least 56% identity to SEQ ID NO: 1.
  • Protein engineered variants can be produced using standard procedures well-known to those skilled in the art. Multiple amino acid substitutions can be made and tested using known methods of mutagenesis, recombination and/or shuffling followed by a relevant screening procedure.
  • these methods involve simultaneously randomizing two or more positions in a polypeptide, or recombination/shuffling of different mutations followed by selecting a polypeptide for functionality, and then sequencing the mutagenized polypeptides to determine the spectrum of allowable substitutions at each position.
  • Other methods that can be used include phage display and region- directed mutagenesis. Mutagenesis/shuffling methods as disclosed above can be combined with high-throughput, automated screening methods to detect activity of cloned, mutagenized polypeptides in host cells. Mutagenized DNA molecules that encode active polypeptides can be recovered from the host cells and rapidly sequenced using modern equipment.
  • substitutions, insertions and deletions to the other regions of the polypeptide chain may be beneficial in enhancing performance of the enzyme in a cleaning and/or treatment composition.
  • substitutions to surface residues in order to change the charge of the enzyme and hence influence its deposition onto surfaces such as textiles, skin or hard surfaces.
  • Other changes may be beneficial in reducing the sensitivity of the enzyme to autolysis, or attack by other proteases, for example by substituting sites that are susceptible to proteolytic attack.
  • Other changes may be beneficial in reducing the sensitivity of the enzyme to denaturation by temperature, surfactant, chelating agent or bleaching agents.
  • a cleaning and/or treatment composition comprising a fungal serine protease selected from the group consisting of a fungal serine protease having:
  • SEQ ID NO: 1 a) SEQ ID NO: 1; b) a fungal serine protease having at least 56%, 70%, 75%, 80%, 85%, 90%, 95%, 99%, identity to SEQ ID NO: 1; and
  • said cleaning and/or treatment composition comprises, based on total composition weight, from about 0.00001% to about 2%, from about 0.0001% to about 1%, from about 0.0005% to about 1%, from about 0.001% to about 0.5% or even from about 0.002% to about 0.25% of said fungal serine protease.
  • said a fungal serine protease has at least 56%, 70%, 75%, 80%, 85%, 90%, 95%, 99%, identity to SEQ ID NO: 1 is selected from the group consisting of fungal serine proteases having SEQ ID NO: 2, SEQ ID NO: 3; SEQ ID NO: 4 and mixtures there of.
  • said additional cleaning material is being selected from the group consisting of surfactants, chelating agents, dye transfer inhibiting agents, dispersants, additional enzymes, and enzyme stabilizers, catalytic materials, bleaching agents, polymeric dispersing agents, clay soil removal/anti-redeposition agents, brighteners, suds suppressors, dyes, perfumes, perfume microcapsules, structure elasticizing agents, fabric softeners, carriers, hydro tropes, processing aids, solvents, pigments, hueing agents, photobleaches, structurants, and mixtures thereof.
  • said cleaning and/or treatment composition comprises an additional enzyme.
  • said additional enzyme is selected from the group consisting of hemicellulases, peroxidases, proteases, cellulases, xylanases, lipases, phospholipases, perhydrolases, esterases, cutinases, pectinases, mannanases, pectate lyases, keratinases, reductases, oxidoreductases, phenoloxidases, lipoxygenases, ligninases, pullulanases, tannases, pentosanases, glucanases, arabinosidases, hyaluronidase, chondroitinase, laccase, amylases, and mixtures thereof.
  • said additional enzyme is selected from the group consisting of: first cycle lipases; cutinases; alpha- amylases; bacterial proteases; microbial-derived endoglucanases; and mixtures thereof.
  • said cleaning and/or treatment composition comprises a surfactant, selected from the group of: anionic surfactants selected from the group consisting of linear alkylbenzene-sulfonate (LAS), alcohol ethoxysulfate (AES), mid-branched alkyl sulfates (HSAS) and mixtures thereof; non ionic alcohol ethoxylates, amine oxides; and mixtures thereof.
  • LAS linear alkylbenzene-sulfonate
  • AES alcohol ethoxysulfate
  • HSAS mid-branched alkyl sulfates
  • said cleaning and/or treatment composition comprising a polymer, selected from the group consisting of polyacrylates; maleic/acrylic acid copolymers; cellulose-derived polymers; polyethyleneimine polymer; and mixtures thereof.
  • said cleaning and/or treatment composition comprises a fabric hueing agent selected from the group consisting of dyes; dye-clay conjugates comprising at least one cationic/basic dye and a smectite clay; and mixtures thereof.
  • said cleaning and/or treatment composition comprises, based on total product weight, from about 0.00003% to about 0.3% hueing agent.
  • said cleaning and/or treatment composition comprises, based on total product weight, less than 15% builder.
  • said cleaning and/or treatment composition is a multi-compartment unit dose.
  • said cleaning and/or treatment composition is in the form of a multi-compartment unit dose, wherein the fungal serine protease is in a different compartment to any additional enzymes and/or chelant.
  • said cleaning and/or treatment composition is a hand dishwashing or machine dishwashing composition.
  • said cleaning and/or treatment composition comprises, based on total cleaning and/or treatment composition weight, a total of no more than 20% water, a total of no more than 15% water or even a total of no more than 10% water.
  • said cleaning and/or treatment composition comprises based on total cleaning and/or treatment composition weight, from about 10% to about 70%, or even from about 20% to about 60% of a water-miscible organic solvent, said water-miscible organic solvent in one aspect having a molecular weight of greater than 70 Daltons, in one aspect said water-miscible organic solvent in one aspect having a molecular weight of greater than 70 Daltons to about 1000 Daltons.
  • said cleaning and/or treatment composition comprising a perfume microcapsule comprising a core and a shell that encapsulates said core, said perfume
  • microcapsule having a D[4,3] average particle of from about 0.01 microns to about 200 microns.
  • the aforementioned additional enzyme may be selected from the group consisting of: lipases, including "first cycle lipases" described in U.S. Patent 6,939,702 Bl (SEQ ID No.2 in the present specification being equivalent to SEQ ID NO:l in U.S. Patent 6,939,702 Bl), a variant of SEQ ID No. 2, a variant of SEQ ID No. 2 having at least 90% identity to SEQ ID No. 2 comprising a substitution of an electrically neutral or negatively charged amino acid with R or K at any of positions 3, 224, 229, 231 and 233, or even a variant comprising T231R and N233R mutations, such variant being sold under the tradename Lipex®; cutinases defined by E.C.
  • lipases including "first cycle lipases” described in U.S. Patent 6,939,702 Bl (SEQ ID No.2 in the present specification being equivalent to SEQ ID NO:l in U.S. Patent 6,939,702 Bl)
  • Class 3.1.1.73 preferably displaying at least 90%, or 95%, or most preferably at least 98% identity with a wild-type derived from one of Fusarium solani, Pseudomonas mendocina or Humicola insolens; alpha-amylases, including a variant of SEQ ID No. 3 (SEQ ID No. 3 corresponding to SEQ ID No. 2 in U.S. patent 5,856,164), a variant of SEQ ID No. 3 having at least 90% identity to SEQ ID No.
  • subtilisins (EC 3.4.21.62), including those derived from Bacillus lentus, B. alkalophilus, B. subtilis, B.
  • bacterial polypeptide endogenous to a member of the genus Bacillus which has a sequence of at least 90%, 94%, 97% and even 99% identity to SEQ ID NO:2 in US 2005/0112749 Al - such an enzyme being commercially available under the tradename CellucleanTM by Novozymes A/S, and mixtures thereof; oxidoreductases, for example oxidases such as glucose, choline or carbohydrate oxidases, oxygenases, catalases, peroxidases, like halo-, chloro-, bromo-, lignin-, glucose- or manganese-peroxidases, dioxygenases or laccases
  • oxidases such as glucose, choline or carbohydrate oxidases, oxygenases, catalases, peroxidases, like halo-, chloro-, bromo-, lignin-, glucose- or manganese-peroxidases, dioxygenases or laccases
  • Suitable commercial products are sold under the Guardzyme® and Denilite® ranges from Novozymes.
  • organic, for example, aromatic compounds are incorporated with the bleaching enzyme. While not being bound by theory, it is believed that these compounds interact with the bleaching enzyme to enhance the activity of the oxidoreductase (enhancer) or to facilitate the electron flow (mediator) between the oxidizing enzyme and the stain typically over strongly different redox potentials; perhydrolases which catalyse the formation of peracids from an ester substrate and peroxygen source.
  • Suitable perhydrolases include variants of the Mycobacterium smegmatis perhydrolase, variants of so-called CE-7 perhydrolases, and variants of wild-type subtilisin Carlsberg possessing perhydrolase activity.
  • any of the aspects of the cleaning and/or treatment compositions described in the present specification may comprise a surfactant, including a surfactant selected from the group of anionic surfactants including anionic surfactants selected from the group consisting of linear
  • alkylbenzene-sulfonate LAS
  • alcohol ethoxysulfate AES
  • HSAS mid-branched alkyl sulfates
  • non-ionic surfactants including alcohol ethoxylates, for example alcohol ethoxylates having a chain length of from 1 to 14 carbons, or 12 to 14 carbons
  • amine oxides and mixtures thereof.
  • any of the aspects of the cleaning and/or treatment compositions described in the present specification may comprise a polymer, including polymers selected from the group consisting of polyacrylates, maleic/acrylic acid copolymers, cellulose-derived polymers, including
  • carboxymethylcellulose and methyl hydroxyethylcellulose carboxymethylcellulose and methyl hydroxyethylcellulose, polyethyleneimine polymers and mixtures thereof.
  • any of the aspects of said cleaning and/or treatment compositions described in the present specification may comprise a builder selected from the group consisting of citric acid, C12-C18 fatty acid, aluminosilicates, including zeolites A, X and/or Y, sodium tripolyphosphate and mixtures thereof.
  • a builder selected from the group consisting of citric acid, C12-C18 fatty acid, aluminosilicates, including zeolites A, X and/or Y, sodium tripolyphosphate and mixtures thereof.
  • any of the aspects of the cleaning and/or treatment compositions described in the present specification may comprise a fabric hueing agent selected from the group consisting of direct violet 7 , direct violet 9 , direct violet 11, direct violet 26, direct violet 31, direct violet 35, direct violet 40, direct violet 41, direct violet 51, direct violet 66, direct violet 99, acid violet 50, acid blue 9, acid violet 17, acid black 1, acid red 17, acid blue 29, solvent violet 13, disperse violet 27 disperse violet 26, disperse violet 28, disperse violet 63 and disperse violet 77, basic blue 16, basic blue 65, basic blue 66, basic blue 67, basic blue 71, basic blue 159, basic violet 19, basic violet 35, basic violet 38, basic violet 48; basic blue 3 , basic blue 75, basic blue 95, basic blue 122, basic blue 124, basic blue 141, thiazolium dyes, reactive blue 19, reactive blue 163, reactive blue 182, reactive blue 96, polymeric dyes such as Liquitint® Violet DD (Milliken), Liquitint® Violet CT (Milliken
  • hueing dye-photobleach conjugates such as the conjugate of sulphonated zinc phthalocyanine with direct violet 99.
  • a particularly preferred hueing agent is a combination of acid red 52 and acid blue 80, or the combination of direct violet 9 and solvent violet 13, dye-clay conjugates comprising at least one cationic basic dye and a smectite clay and mixtures thereof.
  • any of the aspects of the cleaning and/or treatment compositions described in the present specification may comprise, based on total product weight, from about 0% to about 3%, from about 0.0001% to about 0.5%, or even from about 0.0005% to about 0.3% photobleach and/or from about 0.00003% to about 0.3%, from about 0.00008% to about 0.05%, or even from about 0.0001% to about 0.04% hueing agent.
  • any of the aspects of the cleaning and/or treatment compositions described in the present specification may comprise any combinations of materials and parameters disclosed herein.
  • the cleaning and/or treatment compositions described in the present specification may comprise multiple materials, for example, enzymes, surfactants, polymers builders and fabric hueing agents.
  • Enzymes suitable for use in the present cleaning and/or treatment compositions can be obtained from Genencor International, Palo Alto, California, U.S. A; Novozymes A/S, Bagsvaerd, Denmark; Sigma-Aldrich Company Ltd, Dorset, UK; and AB Enzymes, Darmstadt, Germany.
  • Surfactants suitable for use in the present cleaning and/or treatment compositions can be obtained from Stepan, Northfield, Illinois, USA; Huntsman, Salt Lake City, Utah, USA; Procter & Gamble Chemicals, Cincinnati, Ohio, USA.
  • Builders suitable for use in the present cleaning and/or treatment compositions can be obtained from Rhodia, Paris, France; Industrial Zeolite (UK) Ltd, Grays, Essex, UK; Koma, Nestemica, Czech Republic.
  • Polymers suitable for use in the present cleaning and/or treatment compositions can be obtained from BASF, Ludwigshafen, Germany, CP Kelco, Arnhem, Netherlands.
  • Photobleaches suitable for use in the present cleaning and/or treatment compositions can be obtained from Aldrich, Milwaukee, Wisconsin, USA; Frontier Scientific, Logan, Utah, USA; Ciba Specialty Chemicals, Basel, Switzerland; BASF, Ludwigshafen, Germany; Lamberti S.p.A, Gallarate, Italy; Dayglo Color Corporation, Mumbai, India; Organic Dyestuffs Corp., East Buffalo, Rhode Island, USA.
  • Hueing agents suitable for use in the present cleaning and/or treatment compositions can be obtained from Aldrich, Milwaukee, Wisconsin, USA; Ciba Specialty Chemicals, Basel, Switzerland; BASF, Ludwigshafen, Germany; Dayglo Color Corporation, Mumbai, India;
  • adjuncts illustrated hereinafter are suitable for use in the instant compositions and may be desirably incorporated in certain embodiments of the invention, for example to assist or enhance cleaning performance, for treatment of the substrate to be cleaned, or to modify the aesthetics of the cleaning composition as is the case with perfumes, colorants, dyes or the like.
  • Such adjunct are in addition to the materials already disclosed for use in the cleaning and/or treatment compositions described in the present specification. The precise nature of these additional components, and levels of incorporation thereof, will depend on the physical form of the composition and the nature of the cleaning operation for which it is to be used.
  • Suitable adjunct materials include, but are not limited to, additional surfactants, additional builders, additional polymers, additional hueing agents, additional photobleaches, chelating agents, dye transfer inhibiting agents, dispersants, additional enzymes, and enzyme stabilizers, catalytic materials, bleach activators, hydrogen peroxide, sources of hydrogen peroxide, preformed peracids, polymeric dispersing agents, clay soil removal/anti-redeposition agents, brighteners, suds suppressors, dyes, perfumes, structure elasticizing agents, fabric softeners, carriers, hydro tropes, processing aids, solvents, additional hueing agents, structurants and/or pigments.
  • suitable examples of such other adjuncts and levels of use are found in U.S. Patent Nos. 5,576,282, 6,306,812 Bl and 6,326,348 Bl that are incorporated by reference.
  • adjunct ingredients are not essential to Applicants' compositions.
  • certain embodiments of Applicants' compositions do not contain one or more of the following adjuncts materials: additional surfactants, additional builders, additional polymers, additional photobleaches, chelating agents, dye transfer inhibiting agents, dispersants, additional enzymes, and enzyme stabilizers, catalytic materials, bleach activators, hydrogen peroxide, sources of hydrogen peroxide, preformed peracids, polymeric dispersing agents, clay soil removal/anti- redeposition agents, brighteners, suds suppressors, dyes, perfumes, structure elasticizing agents, fabric softeners, carriers, hydrotropes, processing aids, solvents, additional hueing agents, structurants and/or pigments.
  • one or more adjuncts may be present as detailed below:
  • the cleaning compositions of the present invention may comprise one or more bleaching agents.
  • Suitable bleaching agents other than bleaching catalysts include photobleaches, bleach activators, hydrogen peroxide, sources of hydrogen peroxide, pre-formed peracids and mixtures thereof.
  • the compositions of the present invention may comprise from about 0.1% to about 50% or even from about 0.1% to about 25% bleaching agent by weight of the subject cleaning composition.
  • suitable bleaching agents include:
  • photobleaches being selected from the group consisting of xanthene dyes and mixtures thereof; sulfonated zinc phthalocyanine, sulfonated aluminium phthalocyanine, Eosin Y, Phoxine B, Rose Bengal, C.I. Food Red 14 and mixtures thereof; water soluble phthalocyanine;
  • Suitable preformed peracids include, but are not limited to, compounds selected from the group consisting of percarboxylic acids (for example phthalimidoperoxycaproic acid) and salts, percarbonic acids and salts, perimidic acids and salts, peroxymonosulfuric acids and salts, for example, Oxone®, and mixtures thereof.
  • percarboxylic acids for example phthalimidoperoxycaproic acid
  • salts percarbonic acids and salts
  • perimidic acids and salts peroxymonosulfuric acids and salts
  • Oxone® peroxymonosulfuric acids and salts
  • inorganic perhydrate salts including alkali metal salts such as sodium salts of perborate (usually mono- or tetra-hydrate), percarbonate, persulphate, perphosphate, persilicate salts and mixtures thereof.
  • the inorganic perhydrate salts are selected from the group consisting of sodium salts of perborate, percarbonate and mixtures thereof.
  • inorganic perhydrate salts are typically present in amounts of from 0.05 to 40 wt%, or 1 to 30 wt% of the overall composition and are typically incorporated into such compositions as a crystalline solid that may be coated. Suitable coatings include, inorganic salts such as alkali metal silicate, carbonate or borate salts or mixtures thereof, or organic materials such as water-soluble or dispersible polymers, waxes, oils or fatty soaps; and
  • suitable leaving groups are benzoic acid and derivatives thereof - especially benzene sulphonate.
  • Suitable bleach activators include dodecanoyl oxybenzene sulphonate, decanoyl oxybenzene sulphonate, decanoyl oxybenzoic acid or salts thereof, 3,5,5-trimethyl hexanoyloxybenzene sulphonate, tetraacetyl ethylene diamine (TAED) and nonanoyloxybenzene sulphonate (NOBS).
  • TAED tetraacetyl ethylene diamine
  • NOBS nonanoyloxybenzene sulphonate
  • Suitable bleach activators are also disclosed in WO 98/17767. While any suitable bleach activator may be employed, in one aspect of the invention the subject cleaning composition may comprise NOBS, TAED or mixtures thereof.
  • the peracid and/or bleach activator is generally present in the composition in an amount of from about 0.1 to about 60 wt%, from about 0.5 to about 40 wt % or even from about 0.6 to about 10 wt% based on the composition.
  • One or more hydrophobic peracids or precursors thereof may be used in combination with one or more hydrophilic peracid or precursor thereof.
  • the amounts of hydrogen peroxide source and peracid or bleach activator may be selected such that the molar ratio of available oxygen (from the peroxide source) to peracid is from 1:1 to 35:1, or even 2:1 to 10:1.
  • the cleaning compositions according to the present invention may comprise a surfactant or surfactant system wherein the surfactant can be selected from nonionic surfactants, anionic surfactants, cationic surfactants, ampholytic surfactants, zwitterionic surfactants, semi-polar nonionic surfactants and mixtures thereof.
  • surfactant is typically present at a level of from about 0.1% to about 60%, from about 1% to about 50% or even from about 5% to about 40% by weight of the subject composition.
  • the cleaning compositions of the present invention may comprise one or more detergent builders or builder systems.
  • Builders include, but are not limited to, the alkali metal, ammonium and alkanolammonium salts of polyphosphates, alkali metal silicates, alkaline earth and alkali metal carbonates, aluminosilicate builders and polycarboxylate compounds, ether hydroxypolycarboxylates, copolymers of maleic anhydride with ethylene or vinyl methyl ether, 1, 3, 5-trihydroxy benzene-2, 4, 6-trisulphonic acid, and carboxymethyloxysuccinic acid, the various alkali metal, ammonium and substituted ammonium salts of polyacetic acids such as ethylenediamine tetraacetic acid and nitrilotriacetic acid, as well as polycarboxylates such as mellitic acid, succinic acid, citric acid, oxydisuccinic acid, polymaleic acid, benzene 1,3,5-
  • the cleaning compositions herein may contain a chelating agent.
  • Suitable chelating agents include copper, iron and/or manganese chelating agents and mixtures thereof.
  • the subject composition may comprise from about 0.005% to about 15% or even from about 3.0% to about 10% chelating agent by weight of the subject composition.
  • the cleaning compositions of the present invention may also include one or more dye transfer inhibiting agents.
  • Suitable polymeric dye transfer inhibiting agents include, but are not limited to, polyvinylpyrrolidone polymers, polyamine N- oxide polymers, copolymers of N-vinylpyrrolidone and N-vinylimidazole, polyvinyloxazolidones and polyvinylimidazoles or mixtures thereof.
  • the dye transfer inhibiting agents may be present at levels from about 0.0001% to about 10%, from about 0.01% to about 5% or even from about 0.1% to about 3% by weight of the composition.
  • Brighteners - The cleaning compositions of the present invention can also contain additional components that may tint articles being cleaned, such as fluorescent brighteners.
  • Suitable fluorescent brightener levels include lower levels of from about 0.01, from about 0.05, from about 0.1 or even from about 0.2 wt % to upper levels of 0.5 or even 0.75 wt %.
  • compositions of the present invention can also contain dispersants.
  • Suitable water-soluble organic materials include the homo- or co-polymeric acids or their salts, in which the polycarboxylic acid comprises at least two carboxyl radicals separated from each other by not more than two carbon atoms.
  • Enzymes - The cleaning compositions can comprise one or more enzymes which provide cleaning performance and/or fabric care benefits.
  • suitable enzymes include, but are not limited to, hemicellulases, peroxidases, proteases, cellulases, xylanases, lipases, phospholipases, esterases, cutinases, pectinases, mannanases, pectate lyases, keratinases, reductases, oxidases, phenoloxidases, lipoxygenases, ligninases, pullulanases, tannases, pentosanases, glucanases, arabinosidases, hyaluronidase, chondroitinase, laccase, amylases, or mixtures thereof.
  • a typical combination is an enzyme cocktail that may comprise, for example, a protease and lipase in conjunction with amylase.
  • the aforementioned additional enzymes may be present at levels from about 0.00001% to about 2%, from about 0.0001% to about 1% or even from about 0.001% to about 0.5% enzyme protein by weight of the composition.
  • Enzyme Stabilizers - Enzymes for use in detergents can be stabilized by various techniques.
  • the enzymes employed herein can be stabilized by the presence of water-soluble sources of calcium and/or magnesium ions in the finished compositions that provide such ions to the enzymes.
  • a reversible protease inhibitor such as a boron compound, for example, 4-formyl-phenylboronic acid can be added to further improve stability.
  • Catalytic Metal Complexes - Applicants' cleaning compositions may include catalytic metal complexes.
  • One type of metal-containing bleach catalyst is a catalyst system comprising a transition metal cation of defined bleach catalytic activity, such as copper, iron, titanium, ruthenium, tungsten, molybdenum, or manganese cations, an auxiliary metal cation having little or no bleach catalytic activity, such as zinc or aluminum cations, and a sequestrate having defined stability constants for the catalytic and auxiliary metal cations, particularly ethylenediaminetetraacetic acid, ethylenediaminetetra(methylenephosphonic acid) and water- soluble salts thereof.
  • Such catalysts are disclosed in U.S. 4,430,243.
  • compositions herein can be catalyzed by means of a manganese compound.
  • a manganese compound Such compounds and levels of use are well known in the art and include, for example, the manganese-based catalysts disclosed in U.S. 5,576,282.
  • Cobalt bleach catalysts useful herein are known, and are described, for example, in U.S. 5,597,936; U.S. 5,595,967. Such cobalt catalysts are readily prepared by known procedures, such as taught for example in U.S. 5,597,936, and U.S. 5,595,967.
  • compositions herein may also suitably include a transition metal complex of ligands such as bispidones (WO 05/042532 Al) and/or macropolycyclic rigid ligands - abbreviated as "MRLs".
  • ligands such as bispidones (WO 05/042532 Al) and/or macropolycyclic rigid ligands - abbreviated as "MRLs”.
  • MRLs macropolycyclic rigid ligands
  • Suitable transition-metals in the instant transition-metal bleach catalyst include, for example, manganese, iron and chromium.
  • Suitable MRLs include 5,12-diethyl-l,5,8,12- tetraazabicyclo[6.6.2]hexadecane.
  • Suitable transition metal MRLs are readily prepared by known procedures, such as taught for example in WO 00/32601, and U.S. 6,225,464.
  • Solvents - Suitable solvents include water and other solvents such as lipophilic fluids.
  • suitable lipophilic fluids include siloxanes, other silicones, hydrocarbons, glycol ethers, glycerine derivatives such as glycerine ethers, perfluorinated amines, perfluorinated and hydrofluoroether solvents, low-volatility nonfluorinated organic solvents, diol solvents, other environmentally-friendly solvents and mixtures thereof.
  • compositions of the present invention can be formulated into any suitable form and prepared by any process chosen by the formulator, non-limiting examples of which are described in Applicants' examples and in U.S. 4,990,280; U.S. 20030087791A1; U.S. 20030087790A1; U.S. 20050003983A1;
  • the present invention includes a method for cleaning a situs inter alia a surface or fabric.
  • Such method includes the steps of contacting an embodiment of Applicants' cleaning composition, in neat form or diluted in a wash liquor, with at least a portion of a surface or fabric then optionally rinsing and optionally drying such surface or fabric.
  • the surface or fabric may be subjected to a washing step prior to the aforementioned rinsing step.
  • washing includes but is not limited to, scrubbing, and mechanical agitation. Drying of such surfaces or fabrics may be accomplished by any one of the common means employed either in domestic or industrial settings.
  • Such means include but are not limited to forced air or still air drying at ambient or elevated temperatures at pressures between 5 and 0.01 atmospheres in the presence or absence of electromagnetic radiation, including sunlight, infrared, ultraviolet and microwave irradiation.
  • said drying may be accomplished at temperatures above ambient by employing an iron wherein, for example, said fabric may be in direct contact with said iron for relatively short or even extended periods of time and wherein pressure may be exerted beyond that otherwise normally present due to gravitational force.
  • said drying may be accomplished at temperatures above ambient by employing a dryer. Apparatus for drying fabric is well known and it is frequently referred to as a clothes dryer.
  • the cleaning compositions of the present invention are ideally suited for use in laundry applications.
  • the present invention includes a method for laundering a fabric.
  • the method comprises the steps of contacting a fabric to be laundered with a said cleaning laundry solution comprising at least one embodiment of Applicants' cleaning composition, cleaning additive or mixture thereof.
  • the fabric may comprise most any fabric capable of being laundered in normal consumer use conditions.
  • the solution preferably has a pH of from about 8 to about 10.5.
  • the compositions may be employed at concentrations of from about 500 ppm to about 15,000 ppm in solution.
  • the water temperatures typically range from about 5 °C to about 90 °C.
  • the water to fabric ratio is typically from about 1: 1 to about 30:1.
  • a method of treating and/or cleaning a surface or fabric comprising the steps of optionally washing and/or rinsing said surface or fabric, contacting said surface or fabric with any cleaning and/or treatment composition disclosed herein, then optionally washing and/or rinsing said surface and/or fabric then optionally letting drying said surface or fabric dry and/or actively drying said surface or fabric, is disclosed.
  • a stock culture of parent Trichoderma reesei stain QM9414 is cultivated on 3.9% potato agar slants, in a modified minimal medium with glucose as sole carbon source and increased ammonium sulfate concentration in order to avoid exhaustion of nitrogen in the medium.
  • the parent Trichoderma. reesei strain QM9414 is grown in 750ml Erlenmeyer flasks in this modified medium. Erlenmeyer flasks containing 150 ml of the medium are inoculated with spores from 7-day-old culture. Fed-batch cultivations are carried out at 30°C with shaking at 200 rpm.
  • the glucose concentration is monitored daily and supplemented to 30 ⁇ 4-0 g/1.
  • the pH is adjusted to 6.0 daily by the addition of 10% NaOH.
  • the mycelia are removed by centrifugation and the supernatants are concentrated and then stored at -20°C until analysis.
  • the 25 kDa protease is purified from 8 days culture filtrate by ion exchange
  • Granular laundry detergent compositions designed for hand washing or top-loading washing machines.
  • Granular laundry detergent compositions designed for front-loading automatic washing machines.
  • any of the above compositions is used to launder fabrics at a concentration of 7000 to 10000 ppm in water, 20-90 °C, and a 5:1 watencloth ratio.
  • the typical pH is about 10.
  • the fabrics are then dried.
  • the fabrics are actively dried using a dryer.
  • the fabrics are actively dried using an iron.
  • the fabrics are merely allowed to dry on a line wherein they are exposed to air and optionally sunlight.
  • Random graft copolymer is a polyvinyl acetate grafted polyethylene oxide copolymer having a polyethylene oxide backbone and multiple polyvinyl acetate side chains.
  • the molecular weight of the polyethylene oxide backbone is about 6000 and the weight ratio of the polyethylene oxide to polyvinyl acetate is about 40 to 60 and no more than 1 grafting point per 50 ethylene oxide units.
  • Linear alkylbenzenesulfonate having an average aliphatic carbon chain length Cn-Ci 2 supplied by Stepan, Northfield, Illinois, USA
  • AE3S is C12-15 alkyl ethoxy (3) sulfate supplied by Stepan, Northfield, Illinois, USA
  • AE7 is C12-15 alcohol ethoxylate, with an average degree of ethoxylation of 7, supplied by
  • AE9 is C12-13 alcohol ethoxylate, with an average degree of ethoxylation of 9, supplied by
  • HSAS is a mid-branched primary alkyl sulfate with carbon chain length of about 16-17
  • Sodium tripolyphosphate is supplied by Rhodia, Paris, France
  • Zeolite A is supplied by Industrial Zeolite (UK) Ltd, Grays, Essex, UK
  • Polyacrylate MW 4500 is supplied by BASF, Ludwigshafen, Germany
  • Carboxymethyl cellulose is Finnfix® V supplied by CP Kelco, Arnhem, Netherlands Suitable chelants are, for example, diethylenetetraamine pentaacetic acid (DTP A) supplied by Dow Chemical, Midland, Michigan, USA or Hydroxyethane di phosphonate
  • Whitezyme® are all products of Novozymes, Bagsvaerd, Denmark.
  • Bacterial protease (examples 8-13) described in US 6,312,936 Bl supplied by Genencor International, Palo Alto, California, USA
  • Bacterial protease (examples 14-20) described in US 4,760,025 is supplied by Genencor International, Palo Alto, California, USA
  • Fluorescent Brightener 1 is Tinopal® AMS
  • Fluorescent Brightener 2 is Tinopal® CBS- X
  • Sulphonated zinc phthalocyanine and Direct Violet 9 is Pergasol® Violet BN-Z all supplied by Ciba Specialty Chemicals, Basel, Switzerland
  • Sodium perborate is supplied by Degussa, Hanau, Germany
  • NOBS is sodium nonanoyloxybenzenesulfonate, supplied by Future Fuels, Batesville, Arkansas, USA
  • TAED is tetraacetylethylenediamine, supplied under the Peractive® brand name by Clariant GmbH, Sulzbach, Germany
  • S-ACMC is carboxymethylcellulose conjugated with C.I. Reactive Blue 19, sold by Megazyme, Wicklow, Ireland under the product name AZO-CM-CELLULOSE, product code S- ACMC.
  • Soil release agent is Repel-o-tex® PF, supplied by Rhodia, Paris, France
  • Acrylic Acid/Maleic Acid Copolymer is molecular weight 70,000 and acrylate:maleate ratio 70:30, supplied by BASF, Ludwigshafen, Germany
  • HEDP Hydroxyethane di phosphonate
  • HSAS mid-branched alkyl sulfate as disclosed in US 6,020,303 and US 6,060,443 C12-14 dimethyl Amine Oxide is supplied by Procter & Gamble Chemicals, Cincinnati, Ohio, USA
  • Liquitint® Violet CT is supplied by Milliken, Spartanburg, South Carolina, USA
  • the dimensions and values disclosed herein are not to be understood as being strictly limited to the exact numerical values recited. Instead, unless otherwise specified, each such dimension is intended to mean both the recited value and a functionally equivalent range surrounding that value. For example, a dimension disclosed as “40 mm” is intended to mean “about 40 mm”.

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Detergent Compositions (AREA)

Abstract

Cette invention concerne des compositions, comprenant certaines sérine protéases fongiques, et des procédés de fabrication et d'utilisation de telles compositions, comprenant l'utilisation de telles compositions pour nettoyer et/ou traiter un site.
PCT/US2010/054670 2010-10-29 2010-10-29 Compositions de nettoyage et/ou de traitement comprenant une sérine protéase fongique WO2012057781A1 (fr)

Priority Applications (3)

Application Number Priority Date Filing Date Title
PCT/US2010/054670 WO2012057781A1 (fr) 2010-10-29 2010-10-29 Compositions de nettoyage et/ou de traitement comprenant une sérine protéase fongique
US13/278,201 US20120108488A1 (en) 2010-10-29 2011-10-21 Cleaning And/Or Treatment Compositions
US14/833,516 US20150353870A1 (en) 2010-10-29 2015-08-24 Cleaning and/or treatment compositions

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/US2010/054670 WO2012057781A1 (fr) 2010-10-29 2010-10-29 Compositions de nettoyage et/ou de traitement comprenant une sérine protéase fongique

Publications (1)

Publication Number Publication Date
WO2012057781A1 true WO2012057781A1 (fr) 2012-05-03

Family

ID=44624884

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2010/054670 WO2012057781A1 (fr) 2010-10-29 2010-10-29 Compositions de nettoyage et/ou de traitement comprenant une sérine protéase fongique

Country Status (1)

Country Link
WO (1) WO2012057781A1 (fr)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2712915A1 (fr) * 2012-10-01 2014-04-02 The Procter and Gamble Company Procédés de traitement d'une surface et compositions pour utilisation dudit procédé
WO2016090623A1 (fr) * 2014-12-12 2016-06-16 The Procter & Gamble Company Composition nettoyante liquide
CN107002000A (zh) * 2014-12-12 2017-08-01 宝洁公司 液体清洁组合物

Citations (25)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4430243A (en) 1981-08-08 1984-02-07 The Procter & Gamble Company Bleach catalyst compositions and use thereof in laundry bleaching and detergent compositions
US4760025A (en) 1984-05-29 1988-07-26 Genencor, Inc. Modified enzymes and methods for making same
US4990280A (en) 1988-03-14 1991-02-05 Danochemo A/S Photoactivator dye composition for detergent use
US5576282A (en) 1995-09-11 1996-11-19 The Procter & Gamble Company Color-safe bleach boosters, compositions and laundry methods employing same
US5595967A (en) 1995-02-03 1997-01-21 The Procter & Gamble Company Detergent compositions comprising multiperacid-forming bleach activators
US5597936A (en) 1995-06-16 1997-01-28 The Procter & Gamble Company Method for manufacturing cobalt catalysts
US5679630A (en) 1993-10-14 1997-10-21 The Procter & Gamble Company Protease-containing cleaning compositions
WO1998017767A1 (fr) 1996-10-18 1998-04-30 The Procter & Gamble Company Compositions detergentes
US5856164A (en) 1994-03-29 1999-01-05 Novo Nordisk A/S Alkaline bacillus amylase
US6020303A (en) 1996-04-16 2000-02-01 The Procter & Gamble Company Mid-chain branched surfactants
US6060443A (en) 1996-04-16 2000-05-09 The Procter & Gamble Company Mid-chain branched alkyl sulfate surfactants
WO2000032601A2 (fr) 1998-11-30 2000-06-08 The Procter & Gamble Company Procede de preparation de tetraaza macrocycles pontes transversalement
US6187576B1 (en) 1997-10-13 2001-02-13 Novo Nordisk A/S α-amylase mutants
US6225464B1 (en) 1997-03-07 2001-05-01 The Procter & Gamble Company Methods of making cross-bridged macropolycycles
US6306812B1 (en) 1997-03-07 2001-10-23 Procter & Gamble Company, The Bleach compositions containing metal bleach catalyst, and bleach activators and/or organic percarboxylic acids
US6312936B1 (en) 1997-10-23 2001-11-06 Genencor International, Inc. Multiply-substituted protease variants
US6326348B1 (en) 1996-04-16 2001-12-04 The Procter & Gamble Co. Detergent compositions containing selected mid-chain branched surfactants
US20030087790A1 (en) 2001-08-20 2003-05-08 Unilever Home & Personal Care Usa, Division Of Conopco, Inc. Photobleach speckle and laundry detergent compositions containing it
US20030087791A1 (en) 2001-08-20 2003-05-08 Unilever Home & Personal Care Usa, Division Of Conopco, Inc. Photobleach speckle and laundry detergent compositions containing it
EP1347045A1 (fr) * 2000-12-01 2003-09-24 Newbiotechnic, S.A. Enzyme a activite proteolytique
US20050003983A1 (en) 2002-09-11 2005-01-06 Kim Dong Gyu Complex salt for anti-spotting detergents
WO2005042532A1 (fr) 2003-10-31 2005-05-12 Unilever Plc Ligands derives de bispidone et complexes de ceux-ci utilises pour un blanchiment catalytique
US20050112749A1 (en) 2001-06-06 2005-05-26 Helle Outtrup Endo-beta-1,4-glucanase from bacillus
US6939702B1 (en) 1999-03-31 2005-09-06 Novozymes A/S Lipase variant
WO2011075677A2 (fr) * 2009-12-18 2011-06-23 Novozymes, Inc. Procédés de production de polypeptides dans des mutants de trichoderma présentant une carence en protéase

Patent Citations (25)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4430243A (en) 1981-08-08 1984-02-07 The Procter & Gamble Company Bleach catalyst compositions and use thereof in laundry bleaching and detergent compositions
US4760025A (en) 1984-05-29 1988-07-26 Genencor, Inc. Modified enzymes and methods for making same
US4990280A (en) 1988-03-14 1991-02-05 Danochemo A/S Photoactivator dye composition for detergent use
US5679630A (en) 1993-10-14 1997-10-21 The Procter & Gamble Company Protease-containing cleaning compositions
US5856164A (en) 1994-03-29 1999-01-05 Novo Nordisk A/S Alkaline bacillus amylase
US5595967A (en) 1995-02-03 1997-01-21 The Procter & Gamble Company Detergent compositions comprising multiperacid-forming bleach activators
US5597936A (en) 1995-06-16 1997-01-28 The Procter & Gamble Company Method for manufacturing cobalt catalysts
US5576282A (en) 1995-09-11 1996-11-19 The Procter & Gamble Company Color-safe bleach boosters, compositions and laundry methods employing same
US6020303A (en) 1996-04-16 2000-02-01 The Procter & Gamble Company Mid-chain branched surfactants
US6060443A (en) 1996-04-16 2000-05-09 The Procter & Gamble Company Mid-chain branched alkyl sulfate surfactants
US6326348B1 (en) 1996-04-16 2001-12-04 The Procter & Gamble Co. Detergent compositions containing selected mid-chain branched surfactants
WO1998017767A1 (fr) 1996-10-18 1998-04-30 The Procter & Gamble Company Compositions detergentes
US6306812B1 (en) 1997-03-07 2001-10-23 Procter & Gamble Company, The Bleach compositions containing metal bleach catalyst, and bleach activators and/or organic percarboxylic acids
US6225464B1 (en) 1997-03-07 2001-05-01 The Procter & Gamble Company Methods of making cross-bridged macropolycycles
US6187576B1 (en) 1997-10-13 2001-02-13 Novo Nordisk A/S α-amylase mutants
US6312936B1 (en) 1997-10-23 2001-11-06 Genencor International, Inc. Multiply-substituted protease variants
WO2000032601A2 (fr) 1998-11-30 2000-06-08 The Procter & Gamble Company Procede de preparation de tetraaza macrocycles pontes transversalement
US6939702B1 (en) 1999-03-31 2005-09-06 Novozymes A/S Lipase variant
EP1347045A1 (fr) * 2000-12-01 2003-09-24 Newbiotechnic, S.A. Enzyme a activite proteolytique
US20050112749A1 (en) 2001-06-06 2005-05-26 Helle Outtrup Endo-beta-1,4-glucanase from bacillus
US20030087790A1 (en) 2001-08-20 2003-05-08 Unilever Home & Personal Care Usa, Division Of Conopco, Inc. Photobleach speckle and laundry detergent compositions containing it
US20030087791A1 (en) 2001-08-20 2003-05-08 Unilever Home & Personal Care Usa, Division Of Conopco, Inc. Photobleach speckle and laundry detergent compositions containing it
US20050003983A1 (en) 2002-09-11 2005-01-06 Kim Dong Gyu Complex salt for anti-spotting detergents
WO2005042532A1 (fr) 2003-10-31 2005-05-12 Unilever Plc Ligands derives de bispidone et complexes de ceux-ci utilises pour un blanchiment catalytique
WO2011075677A2 (fr) * 2009-12-18 2011-06-23 Novozymes, Inc. Procédés de production de polypeptides dans des mutants de trichoderma présentant une carence en protéase

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
BELÉN SUÁREZ M ET AL: "Characterization of genes encoding novel peptidases in the biocontrol fungus Trichoderma harzianum CECT 2413 using the TrichoEST functional genomics approach", CURRENT GENETICS ; EUKARYOTES WITH EMPHASIS ON YEASTS, FUNGI, PROTISTS, CELL ORGANELLES, SPRINGER, BERLIN, DE, vol. 51, no. 5, 6 April 2007 (2007-04-06), pages 331 - 342, XP019515006, ISSN: 1432-0983, DOI: 10.1007/S00294-007-0130-5 *
D. DIENES ET AL., ENZYME AND MICROBIAL TECHNOLOGY, vol. 40, 2007, pages 1087
DATABASE UniProt [Online] 1 July 2008 (2008-07-01), "SubName: Full=Trypsin;", XP002661161, retrieved from EBI accession no. UNIPROT:B2WMB3 Database accession no. B2WMB3 *
DATABASE UniProt [online] 29 May 2007 (2007-05-29), "SubName: Full=Serin endopeptidase;", retrieved from EBI accession no. UNIPROT:A4V8W4 Database accession no. A4V8W4 *

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2712915A1 (fr) * 2012-10-01 2014-04-02 The Procter and Gamble Company Procédés de traitement d'une surface et compositions pour utilisation dudit procédé
WO2014055245A1 (fr) * 2012-10-01 2014-04-10 The Procter & Gamble Company Procédés de traitement d'une surface et compositions destinées à être utilisées dans ceux-ci
WO2016090623A1 (fr) * 2014-12-12 2016-06-16 The Procter & Gamble Company Composition nettoyante liquide
CN107002000A (zh) * 2014-12-12 2017-08-01 宝洁公司 液体清洁组合物

Similar Documents

Publication Publication Date Title
US20120108488A1 (en) Cleaning And/Or Treatment Compositions
EP3101110B1 (fr) Enzyme et compositions contenant un agent azurant les tissus
EP1876227B2 (fr) Compositions de lavage
US20110039751A1 (en) Cleaning and/or treatment compositions
CN106414698B (zh) 洗涤剂组合物
EP2285944B1 (fr) Compositions de detergents liquides
US8309338B2 (en) Polypeptides having endoglucanase activity and polynucleotides encoding same
US20070179076A1 (en) Detergent composition
JP5698217B2 (ja) 新規真菌プロテアーゼおよびその使用
MX2011002157A (es) Composiciones de limpieza y/o tratamiento.
US20140038876A1 (en) Detergent Compositions Comprising Mettaloproteases
WO2014055245A1 (fr) Procédés de traitement d'une surface et compositions destinées à être utilisées dans ceux-ci
CN108350400B (zh) 衣物洗涤方法，多肽的用途和洗涤剂组合物
WO1998010060A1 (fr) Variantes de peroxydase
WO2012057781A1 (fr) Compositions de nettoyage et/ou de traitement comprenant une sérine protéase fongique
WO2011026154A2 (fr) Compositions de nettoyage et/ou de traitement
CN113166747A (zh) 蛋白酶变体及其用途
EP4038170A1 (fr) Polypeptides comprenant au moins deux domaines de liaison aux hydrates de carbone
HUE032793T2 (en) Detergent compositions

Legal Events

Date Code Title Description
121 Ep: the epo has been informed by wipo that ep was designated in this application

Ref document number: 10773515

Country of ref document: EP

Kind code of ref document: A1

NENP Non-entry into the national phase

Ref country code: DE

122 Ep: pct application non-entry in european phase

Ref document number: 10773515

Country of ref document: EP

Kind code of ref document: A1