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WO2012048890A1 - Dispositif de tête de sonde et procédé permettant d'établir des diagnostics plus précis - Google Patents

Dispositif de tête de sonde et procédé permettant d'établir des diagnostics plus précis Download PDF

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Publication number
WO2012048890A1
WO2012048890A1 PCT/EP2011/005169 EP2011005169W WO2012048890A1 WO 2012048890 A1 WO2012048890 A1 WO 2012048890A1 EP 2011005169 W EP2011005169 W EP 2011005169W WO 2012048890 A1 WO2012048890 A1 WO 2012048890A1
Authority
WO
WIPO (PCT)
Prior art keywords
probe head
head according
light
detector
light source
Prior art date
Application number
PCT/EP2011/005169
Other languages
English (en)
Inventor
Sune Svanberg
Mikkel Brydegaard
Original Assignee
Spectracure Ab
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Spectracure Ab filed Critical Spectracure Ab
Publication of WO2012048890A1 publication Critical patent/WO2012048890A1/fr

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Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
    • A61B5/00—Measuring for diagnostic purposes; Identification of persons
    • A61B5/0059—Measuring for diagnostic purposes; Identification of persons using light, e.g. diagnosis by transillumination, diascopy, fluorescence

Definitions

  • TITLE Probe head device and method for enhanced
  • This invention pertains in general to the field of non-invasive diagnosis of suspected dermatology lesions, such as skin neoplasms, neurodegeneration, diabetes, chronic kidney disease or premature aging of skin. More particularly the invention relates obtaining and analysing multispectral images or spectral databases from
  • the invention relates to a hand-held probe head to covering a surface area to be investigated using multi-spectral imaging .
  • optical spectroscopy is an effective method for characterisation of composition of material. It also known that spectroscopy is can be used for diagnosis of various conditions, for example of medical nature.
  • multi-spectral imaging may be performed.
  • relevant information may be extracted and shown, for example as a false-colour image.
  • Two phenomena may be used for multi-spectral imaging either reflectance where the objects colours are measured or fluoresces.
  • Fluorosensor Employing Light-Emitting Ultraviolet Diodes as Excitation Sources discloses different systems and devices for diagnosis of lesions, i.e. skin cancer, employing optical fibres wherein the illumination and the collected light are transmitted in the same optical fibre or in a bundle of optical fibres.
  • An important application is to diagnose human skin tissue to discover lesions e.g. rodent ulcer or squamous cell carcinoma. Changes may be discovered using auto- fluorescence or by employing more specific spectroscopic signals from supplied photosensitizers .
  • AGE-signals are related to a number of illnesses, such as neurodegeneration and/or diabetes and/or chronic kidney disease.
  • the diagnostics of malignant tumours is a clinical challenge - frequently a tissue sample (biopsy) has to be taken for microscopic evaluation by an experienced
  • LEDs light emitting diodes
  • LEDs have become readily available at low cost.
  • LEDs are known to have an emission profile being temperature dependent (i.e. unstable) which may cause issues with artefacts which is an issue when used for diagnosis .
  • embodiments of the present invention preferably seek to mitigate, alleviate or eliminate one or more deficiencies, disadvantages or issues in the art, such as the above-identified, singly or in any combination by providing a device and a method that may be used to investigate or diagnosing suspected lesions, according to the appended patent claims.
  • the invention is designed for non-invasive
  • the invention measures the surface optical
  • the hand held probe head is configured to detect backscattered spectra from sequentially illumination of a surface or detect the backscattered light using multispectral imaging. This could be conducted either separately or in combination.
  • a probe head for topical illumination of a tissue sample and collection of backscattered light therefrom.
  • the probe head comprising, a body having a distal end and a proximal end, the distal end arranged for apposition to a surface of the sample for covering a surface area thereof, a light source mounted elevated and inclined in the body for sequentially illuminating a central portion of the surface area, and a detector at said proximal end perpendicularly positioned to detect backscattered light from the central portion.
  • a method for topical illumination of a tissue sample and collection of backscattered light therefrom comprising, providing a body comprising a light source and a detector, positioning the body apposition to the sample for covering a surface area thereof,
  • a dermatology lesion such as neoplasms and/or neurodegeneration and/or diabetes and/or chronic kidney disease and/or premature aging of skin.
  • Some embodiments of the invention also provide for a hand device wherein optical fibers are not employed for transmitting light to and from a tissues site. Instead the light sources and preferably the detector are integrated into the hand-held device. This will lead to less intensity losses than if fibers were being used.
  • Some embodiments of the invention also provide for a hand held device wherein the illumination and detection of light is performed without being in contact with the sample. This is especially an advantage when investigating or diagnosing skin tissue, since artifacts and/or
  • Some embodiments of the invention also provide for a hand-held device utilizing temperature stabilization of a LED emission profile from the current-voltage
  • Some embodiments of the invention also provide for a hand-held device able to investigate or diagnosing for example a suspected dermatology lesion in a present state. It should be emphasized that the term
  • Fig. la and b illustrate that a LED diode's
  • Fig. 2 is showing a vertical cross-section of an exemplary embodiment of a probe head
  • Fig.3 is showing a horizontal cross-section of an exemplary embodiment of a probe head
  • Fig. 4 is showing a 3D illustration of an exemplary body of a probe head. DESCRIPTION OF THE PREFERRED EMBODIMENTS
  • the following description focuses on an embodiment of the present invention applicable to a hand held probe head for non-invasive diagnosis of suspected dermatology lesions and in particular to a multi spectral imaging device.
  • a probe head having illuminating and detecting
  • the probe head may be used to non-invasive measure a skin surface' s real optical characteristic in its present state.
  • the probe head may be used to investigate or diagnosing easily accessible locations, such as human skin. For the invented probe head there are no fibres being in contact with the tissue surface (i.e. skin) for
  • distortions in the spectral signal may also originate from skin humidity which may change the coupling of a spectral signal, especially utilizing fibres in contact with a skin surface .
  • Fig. 2 a vertical cross-section of an exemplary embodiment of a probe head 1 is illustrated.
  • the probe head is made of a body 19 being cupped to create an open-end cavity 8.
  • the cavity 8 becomes an enclosed space.
  • the surface area 7 being covered by the cavity 8 is the surface area 7 to be investigated or diagnosed.
  • the body 10 is having bores 9 to hold light sources 13, 20.
  • Light source 13 is here illustrating a LED or laser diode and light source 20 is illustrating a broad banded light source for reflectance measurements.
  • the light source 13 may be a narrow banded light-source having an emission of from 245 nm and up to about 7 micrometres and an emission width preferable of 10 to 20 nm.
  • the narrow emission may be at 355, 375 or 395 nm.
  • a probe head 1 may in some embodiments only comprise of one or a plurality of narrow light sources 13.
  • the probe head 1 may comprise of only one or a plurality of broad banded light sources 20. Additionally and/or
  • the probe head 1 may comprise any combinations thereof.
  • the detector 11 is here illustrated as a fibre connectable to an external spectrometer or detector.
  • the detector may be connected to the body 19 using a contact 12, such as a SMA contact.
  • the detector 11 may be an integrated to the body 19.
  • the integrated detector may for example be a CCD or CMOS for multispectral imaging.
  • the detector 11 may be an integrated LED or diode detector.
  • a layer 18 being a thermal and/or electric isolating material may be arranged between the body 19 and the surface of sample 17.
  • Layer 18 may be a changeable contact matrix.
  • the light sources 13, 20 are arranged elevated and inclined or angled in relation to the surface area 7. Thereby may at least a portion 16 of the surface area 7 be illuminated by the light sources 13, 20. Portion 16 may be a central part of the surface 7.
  • the detector 11 may be arranged perpendicular to collect backscattered light from the illuminated portion 16. This arrangement is an
  • illuminating light may cause the specular light to not hit the detection surface of the detector.
  • the narrow banded light sources 13 may be used as an excitation source to obtain fluorescence.
  • a short-pass filter 14 may be fitted in front of the light source 13. The short pass filter may eliminate emission components in the light source 13 which may fall within the wavelength range of the detected backscattered fluorescence light.
  • a long-pass filter may be fitted in front of the detector to block excitation light, such as Ultraviolet light, but permit fluorescence light to pass.
  • polarisation filter may be used in front of lights sources and/or detector .
  • a horizontal cross-section of an exemplary embodiment of a probe head 1 is illustrated.
  • a probe head 1 may have more than one narrow-band light 13a-c sources and/or more than one broadband light source 20a-c. If more than one narrow band light source 13a-c is used, each may have a different narrow emission.
  • each narrow band light source 13a-c may be fitted with an optical filter 14a-c.
  • Lights sources 13a-c, 20a-c may be arranged elevated and inclined or angled in a "ceiling" of a cavity.
  • the Light sources 13a-c, 20a-c should preferably be configured to direct emission of light towards the same portion 16 of the sample area 7 (for reference see Fig 2) .
  • a detector 11 is centrally and perpendicularly arranged to detect the backscattered light from the illuminated portion of the surface being covered by body 19.
  • the body 11 may be fitted with a heat sink 23 for temperature stabilisation of the probe head 1.
  • a Peltier cooling element may also be mounted for increasing the temperature stabilisation of the probe head 1.
  • FIG. 3 a 3D illustration of an exemplary body 49 of a probe head 1 is shown.
  • a detector may be fitted in a central bore 41.
  • the central bore 41 may in some
  • inventions also be configured with an element 51 for positioning a long-pass optical filter in front of the detector.
  • the body 49 may also have bores for receiving light sources 43, 50.
  • the light source receiving bores 43, 50 may be arranged around central bore 41.
  • the light source receiving bores 43, 50 may be configured to direct the emitted light to the same spot being a portion of a surface area covered by the body 49.
  • the bores 43 for receiving narrow banded light sources may be configured with an element 44 for positioning a short-pass optical filter in front of the light source.
  • the bores 43 for receiving narrow banded light sources may be configured with an element 44 for positioning a short-pass optical filter in front of the light source.
  • the body 49 may be configured to hold a heat sink at position 53.
  • LEDs are a cheap but there emission spectra may be affected by the temperature of the diode. This may give difficulties to evaluate data.
  • One way of overcome this is to mount the LEDs on a temperature stabilising heat sink.
  • Another alternative is to monitor the real light emission which may be used for a compensation of the spectral shift.
  • the inventor has shown (illustrated in fig. la and lb) that a diode's temperature and thereby the real
  • emission profile may be established from a diode's current- voltage characteristic.
  • the plots 5 and 6 in fig la and b illustrates how the current-voltage characteristics change for a LED having different temperatures T. Thus this current voltage characteristic may be used for
  • the current- voltage characteristic may easily be determined from the drive circuit of the diode.
  • LED with an emission width of 10-20nm may replace relatively expensive narrow laser diodes .
  • LEDs make it possible to employ a number of LEDS as light sources for sequentially excitation of fluorescence using a plurality of various wavelengths. By using more than one LED each with a different emission wavelength, more
  • LEDS Another possibility with LEDS is to employ broad-banded white-light LEDS for investigation of reflection characteristics (colour- characteristics) by using spectroscopy.
  • At least a portion of the surface covered by the probe head is illuminated with light in an oblique angle. Thereby may specular reflexions
  • Specular reflexions are tangible when surfaces are humid.
  • a plurality of light sources may be used sequentially where backscattered light may be collected for each of the light sources.
  • the collection of light may be conducted in a spectral mode using a detector.
  • more than one detector may be used to collect the backscattered light.
  • the detector may be at least one fibre connected to the entrance slit of an external spectrometer.
  • the detection may be conducted in a multi-spectral mode using a CCD or CMOS detector, such as a camera.
  • a CCD or CMOS detector such as a camera.
  • the detector may register backscattered light from
  • the collected backscattered light may be reflected light.
  • the collected backscattered light may be fluorescence light.
  • laser diodes or LEDs may be modulated very fast using their drive current. Phase-shifts and demodulation of the detected light may then be employed to investigate the life-time of studied molecules' excited states. Further, diffusion processes within a sample may be investigated which may enhance diagnostic and
  • the body of the probe head is configured to
  • the shape and/or configuration of the body ensure that background light or ambient light is debarred from the enclosed cavity which is obtained when the body is positioned to cover an area of a surface to be investigated.
  • the light sources may illuminate the portion of the surface area to be investigated from the "ceiling" and/or the walls of the cavity.
  • the body of the probe may be made of a material having optical characteristics to allow an investigator (i.e. a medical practitioner) to see through the body of the probe head while the body still debar interfering background light or ambient light.
  • the contact surface between the body of the probe head and the surface may in some embodiments be a layer of a thermal and/or electrical isolating material to isolate the body of the probe head from the surface. This may increase the temperature stability of the emission profile of the light sources.
  • the layer may be a contact matrix which may be exchanged between each investigation.
  • the layer may be of a material easy to clean or made aseptic. If necessary the material of the isolating layer may be of a material which could be sterilised.
  • LEDs Light-Emitting Diods
  • the LEDs may have emission wavelengths ranging from 330 nm to 1.2 micrometers to illuminate a surface where for example a suspected tumor is located.
  • the surface may be sequentially imaged from reflected light by activating LEDs, one after the other. For each LED recording an image using a suitable imaging system with a suitable detector. A normal digital camera operating in black-and white mode, optionally with the IR filter removed, may be used.
  • the LEDs may irradiate the surface to be studied inside a small enclosure or cavity, typically an openen-ended can-shaped enclosure or cupped body, where the opening is pressed against the tissue to block out ambient light.
  • a rubber or disposable cushion material may provide the light-tight coupling to the tissue.
  • the detector i.e. a camera
  • the detector may be placed with its optical aperture in the opposite end of the enclosure or cavity.
  • the LEDs should be appropriately shielded to avoid stray light from the LEDs entering the detector directly. Preferably only light reflection from the surface should be collected by the detector.
  • this type of image recording would extend the spectral range of the eye (400-700 nm) substantially both towards short wavelengths, and in particular to near-IR
  • a plurality of images may be treated using
  • UV-LEDs may also be used in combination with
  • fluorescence images may be included in the analysis to isolate the sought after features .
  • the multispectral imaging device as described herein may be incorporated in a hand-held unit, which the dermatologist may hold against a suspicious skin area .
  • a device according to the present invention may in some
  • configurations also be used for other tasks, such as industrial inspection, detection of forgery in forensic sciences, to visualize early signs of agricultural diseases in leaves, components for circuit cards etc.
  • the invented device may be coupled to a compact separate computer for image analyses.
  • the device may have an integrated dedicated processor in the hand-held unit, providing real-time display of the features being under investigation. While LED:s are very suitable devices for the illumination of the sample, other light sources such as filtered incandescent lamps, diode lasers, semiconductor laser etc may be anticipated for the illumination .

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Medical Informatics (AREA)
  • Biophysics (AREA)
  • Pathology (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Heart & Thoracic Surgery (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Surgery (AREA)
  • Animal Behavior & Ethology (AREA)
  • General Health & Medical Sciences (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)

Abstract

La présente invention concerne une tête de sonde destinée à éclairer de manière topique un échantillon de tissu et à collecter la lumière rétrodiffusée de celui-ci. La tête de sonde comprend un corps ayant une extrémité distale et une extrémité proximale, l'extrémité distale étant configurée pour être apposée sur une surface de l'échantillon de manière à couvrir une aire de surface de celui-ci, une source de lumière montée élevée et inclinée dans ledit corps pour séquentiellement éclairer une partie centrale de l'aire de surface, un détecteur au niveau d'une extrémité proximale et positionné perpendiculairement pour détecter la lumière rétrodiffusée de la partie centrale.
PCT/EP2011/005169 2010-10-14 2011-10-14 Dispositif de tête de sonde et procédé permettant d'établir des diagnostics plus précis WO2012048890A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US39297910P 2010-10-14 2010-10-14
US61/392,979 2010-10-14

Publications (1)

Publication Number Publication Date
WO2012048890A1 true WO2012048890A1 (fr) 2012-04-19

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Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AT514987A1 (de) * 2013-11-05 2015-05-15 H & P Trading Gmbh Messkopf
WO2016063063A1 (fr) * 2014-10-22 2016-04-28 University Hospitals Of Leicester Nhs Trust Appareil de diagnostic
WO2017194993A1 (fr) * 2016-05-10 2017-11-16 Synaptive Medical (Barbados) Inc. Imagerie multispectrale synchronisée

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE2630486A1 (de) * 1976-07-07 1978-01-19 Dornier System Gmbh Narkoseueberwachungssensor
US6122042A (en) * 1997-02-07 2000-09-19 Wunderman; Irwin Devices and methods for optically identifying characteristics of material objects

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE2630486A1 (de) * 1976-07-07 1978-01-19 Dornier System Gmbh Narkoseueberwachungssensor
US6122042A (en) * 1997-02-07 2000-09-19 Wunderman; Irwin Devices and methods for optically identifying characteristics of material objects

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
MIKKEL BRYDEGAARD ET AL: "Broad-band multispectral microscope for imaging transmission spectroscopy employing an array of light-emitting diodes", AMERICAN JOURNAL OF PHYSICS, AMERICAN ASSOCIATION OF PHYSICS TEACHERS, US, vol. 77, no. 2, 1 February 2009 (2009-02-01), pages 104 - 110, XP009156522, ISSN: 0002-9505 *

Cited By (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AT514987A1 (de) * 2013-11-05 2015-05-15 H & P Trading Gmbh Messkopf
AT514987B1 (de) * 2013-11-05 2015-07-15 H & P Trading Gmbh Messkopf
WO2016063063A1 (fr) * 2014-10-22 2016-04-28 University Hospitals Of Leicester Nhs Trust Appareil de diagnostic
WO2017194993A1 (fr) * 2016-05-10 2017-11-16 Synaptive Medical (Barbados) Inc. Imagerie multispectrale synchronisée
GB2566844A (en) * 2016-05-10 2019-03-27 Synaptive Medical Barbados Inc Multispectral synchronized imaging
US11013414B2 (en) 2016-05-10 2021-05-25 Synaptive Medical Inc. Multispectral synchronized imaging
GB2566844B (en) * 2016-05-10 2022-01-05 Synaptive Medical Inc Multispectral synchronized imaging

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