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WO2010010096A3 - Procédé de production de plantes haploïdes, haploïdes doublées et/ou dihaploïdes, par gynogenèse - Google Patents

Procédé de production de plantes haploïdes, haploïdes doublées et/ou dihaploïdes, par gynogenèse Download PDF

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Publication number
WO2010010096A3
WO2010010096A3 PCT/EP2009/059376 EP2009059376W WO2010010096A3 WO 2010010096 A3 WO2010010096 A3 WO 2010010096A3 EP 2009059376 W EP2009059376 W EP 2009059376W WO 2010010096 A3 WO2010010096 A3 WO 2010010096A3
Authority
WO
WIPO (PCT)
Prior art keywords
haploid
plants
embryos
dihaploid
producing
Prior art date
Application number
PCT/EP2009/059376
Other languages
English (en)
Other versions
WO2010010096A2 (fr
Inventor
Muriel Archipiano
Danièle HOSEMANS
Eric Lionneton
Agnès Vermeulen
Original Assignee
Clause
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Clause filed Critical Clause
Priority to US13/055,210 priority Critical patent/US8969658B2/en
Priority to MX2011000828A priority patent/MX2011000828A/es
Priority to ES201190006A priority patent/ES2403155B1/es
Publication of WO2010010096A2 publication Critical patent/WO2010010096A2/fr
Publication of WO2010010096A3 publication Critical patent/WO2010010096A3/fr

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Classifications

    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
    • A01H1/02—Methods or apparatus for hybridisation; Artificial pollination ; Fertility
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
    • A01H1/06—Processes for producing mutations, e.g. treatment with chemicals or with radiation
    • A01H1/08—Methods for producing changes in chromosome number
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H5/00—Angiosperms, i.e. flowering plants, characterised by their plant parts; Angiosperms characterised otherwise than by their botanic taxonomy
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/13—Plant traits

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Developmental Biology & Embryology (AREA)
  • Environmental Sciences (AREA)
  • Botany (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Cell Biology (AREA)
  • Biotechnology (AREA)
  • Engineering & Computer Science (AREA)
  • Physiology (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)

Abstract

L'invention concerne un procédé de production de plantes haploïdes H, haploïdes doublées HD et/ou dihaploïdes DH, les HD et DH étant homozygotes ou essentiellement homozygotes, ce procédé étant du type de ceux relevant de la technique de gynogenèse induite par du pollen irradié. Ce procédé comprend une étape d'irradiation du matériel reproducteur du parent mâle à une dose comprise entre 160 et 190 Gamma Ray et/ou une étape de sélection des plantes haploïdes H et/ou DH par utilisation de marqueur(s) moléculaire(s). L'invention concerne également un procédé de production de plantes haploïdes, haploïdes doublées et/ou dihaploïdes homozygotes comprenant une étape de détermination de la (ou des) dose(s) d'irradiation adéquate(s) pour augmenter les rendements desdites plantes en fonction de facteurs multiples donnés comme l'espèce végétale, les génotypes du parent mâle et du parent femelle, les conditions climatiques et physiologiques, le moment de la récolte des fruits, le niveau de croissance des embryons recueillis en vue de leur culture, le niveau de développement des embryons mis en culture. Par ailleurs, l'invention concerne le(s) marqueur(s) moléculaire(s) utilisé(s) dans l'étape de sélection ainsi que les embryons haploïdes, les embryons dihaploïdes obtenus par le procédé de l'invention, la descendance et les semences des plantes obtenues par le procédé de l'invention.
PCT/EP2009/059376 2008-07-21 2009-07-21 Procédé de production de plantes haploïdes, haploïdes doublées et/ou dihaploïdes, par gynogenèse WO2010010096A2 (fr)

Priority Applications (3)

Application Number Priority Date Filing Date Title
US13/055,210 US8969658B2 (en) 2008-07-21 2009-07-21 Method for producing haploid, doubled haploid and/or dihaploid plants by gynogenesis
MX2011000828A MX2011000828A (es) 2008-07-21 2009-07-21 Procedimiento de produccion de plantas haploides, dobles haploides y/o dihaploides, por ginogenesis.
ES201190006A ES2403155B1 (es) 2008-07-21 2009-07-21 Procedimiento de producción de plantas haploides, dobles haploides y/o dihaploides, por ginogénesis

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
FR0854952A FR2933842A1 (fr) 2008-07-21 2008-07-21 Procede de production de plantes haploides, haploides doublees et/ou dihaploides, par gynogenese
FR0854952 2008-07-21

Publications (2)

Publication Number Publication Date
WO2010010096A2 WO2010010096A2 (fr) 2010-01-28
WO2010010096A3 true WO2010010096A3 (fr) 2010-04-22

Family

ID=40417601

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2009/059376 WO2010010096A2 (fr) 2008-07-21 2009-07-21 Procédé de production de plantes haploïdes, haploïdes doublées et/ou dihaploïdes, par gynogenèse

Country Status (5)

Country Link
US (1) US8969658B2 (fr)
ES (1) ES2403155B1 (fr)
FR (1) FR2933842A1 (fr)
MX (1) MX2011000828A (fr)
WO (1) WO2010010096A2 (fr)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102919185A (zh) * 2012-11-27 2013-02-13 北京市水产科学研究所 一种团头鲂精子诱导锦鲤雌核发育的方法
CN107630100A (zh) * 2016-07-15 2018-01-26 天津市农业质量标准与检测技术研究所 一种基于est‑ssr标记的甜瓜杂交种“玉玲珑”种子纯度鉴定的方法
EP3716756A4 (fr) * 2017-11-29 2021-09-15 Weedout Ltd. Compositions, kits et procédés de lutte contre les mauvaises herbes du genre amaranthus
WO2019215581A1 (fr) 2018-05-06 2019-11-14 Weedout Ltd. Procédés de lutte contre les mauvaises herbes du genre amaranthus
WO2020084586A1 (fr) 2018-10-25 2020-04-30 Weedout Ltd. Procédés d'inhibition de la croissance de mauvaises herbes
CN110278868A (zh) * 2019-05-17 2019-09-27 云南农业大学 一种基于带芽茎段为外植体的黑籽南瓜组培繁殖方法
CN110305800B (zh) * 2019-07-24 2023-03-21 河南省农业科学院植物营养与资源环境研究所 一种食用菌单倍体的无菌快速分离方法
CN111837948B (zh) * 2020-06-24 2022-09-23 中国农业科学院郑州果树研究所 利用西瓜未授粉子房培养获得双单倍体植株的方法
CN113785772A (zh) * 2021-08-30 2021-12-14 江苏省农业科学院 一种葫芦单倍体胚诱导的方法
WO2024036102A2 (fr) * 2022-08-08 2024-02-15 Verda Bio Llc Procédés de sélection de plantes cannabaceae

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2004072259A2 (fr) * 2003-02-11 2004-08-26 Dow Agrosciences Llc Genes fad2 et fad3 modifies dans brassica et detection moleculaire associee assistee par marqueurs

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2004072259A2 (fr) * 2003-02-11 2004-08-26 Dow Agrosciences Llc Genes fad2 et fad3 modifies dans brassica et detection moleculaire associee assistee par marqueurs

Non-Patent Citations (7)

* Cited by examiner, † Cited by third party
Title
DATABASE EMBL [online] 18 February 2004 (2004-02-18), "Cucurbita pepo omega-6 fatty acid desaturase (FAD2) mRNA, complete cds.", XP002550301, retrieved from EBI accession no. EMBL:AY525163 Database accession no. AY525163 *
FROELICHER YANN ET AL: "Induced parthenogenesis in mandarin for haploid production: induction procedures and genetic analysis of plantlets", PLANT CELL REPORTS, vol. 26, no. 7, July 2007 (2007-07-01), pages 937 - 944, XP002519882, ISSN: 0721-7714 *
GONZALO M J ET AL: "Simple-sequence repeat markers used in merging linkage maps of melon (Cucumis melo L.)", THEORETICAL AND APPLIED GENETICS, vol. 110, no. 5, March 2005 (2005-03-01), pages 802 - 811, XP002519884, ISSN: 0040-5752 *
KURTAR E S ET AL: "Obtention of haploid embryos and plants through irradiated pollen technique in squash (Cucurbita pepo L.)", EUPHYTICA, vol. 127, no. 3, 2002, pages 335 - 344, XP002558905, ISSN: 0014-2336 *
SAUTON A ET AL: "INDUCTION OF GYNOGENETIC HAPLOID PLANTS IN MUSKMELON CUCUMIS-MELO L. BY USE OF IRRADIATED POLLEN", AGRONOMIE, PARIS, FR, vol. 7, no. 2, 1 January 1987 (1987-01-01), pages 141 - 147, XP008103758, ISSN: 0249-5627 *
SHALABY TAREK A: "Factors affecting haploid induction through in vitro gynogenesis in summer squash (Cucurbita pepo L.)", SCIENTIA HORTICULTURAE (AMSTERDAM), vol. 115, no. 1, December 2007 (2007-12-01), pages 1 - 6, XP022340589, ISSN: 0304-4238 *
TANG FEIYU ET AL: "In vitro production of haploid and doubled haploid plants from pollinated ovaries of maize (Zea mays)", PLANT CELL TISSUE AND ORGAN CULTURE, vol. 84, no. 2, February 2006 (2006-02-01), pages 233 - 237, XP002519883, ISSN: 0167-6857 *

Also Published As

Publication number Publication date
ES2403155B1 (es) 2014-07-15
ES2403155A2 (es) 2013-05-14
US20110214201A1 (en) 2011-09-01
WO2010010096A2 (fr) 2010-01-28
US8969658B2 (en) 2015-03-03
ES2403155R1 (es) 2013-09-19
MX2011000828A (es) 2011-07-28
FR2933842A1 (fr) 2010-01-22

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