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WO2009020142A1 - リガンド－レセプター結合阻害活性の測定方法 - Google Patents

リガンド－レセプター結合阻害活性の測定方法 Download PDF

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Publication number
WO2009020142A1
WO2009020142A1 PCT/JP2008/064091 JP2008064091W WO2009020142A1 WO 2009020142 A1 WO2009020142 A1 WO 2009020142A1 JP 2008064091 W JP2008064091 W JP 2008064091W WO 2009020142 A1 WO2009020142 A1 WO 2009020142A1
Authority
WO
WIPO (PCT)
Prior art keywords
ligand
inhibitory activity
receptor
measurement
receptor binding
Prior art date
Application number
PCT/JP2008/064091
Other languages
English (en)
French (fr)
Inventor
Yasushi Shimonaka
Original Assignee
Chugai Seiyaku Kabushiki Kaisha
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Chugai Seiyaku Kabushiki Kaisha filed Critical Chugai Seiyaku Kabushiki Kaisha
Priority to EP08792247.2A priority Critical patent/EP2187214B1/en
Priority to ES08792247.2T priority patent/ES2550158T3/es
Priority to US12/672,237 priority patent/US8796039B2/en
Priority to JP2009526470A priority patent/JP5373610B2/ja
Publication of WO2009020142A1 publication Critical patent/WO2009020142A1/ja

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/566—Immunoassay; Biospecific binding assay; Materials therefor using specific carrier or receptor proteins as ligand binding reagents where possible specific carrier or receptor proteins are classified with their target compounds
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54353—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals with ligand attached to the carrier via a chemical coupling agent
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6854—Immunoglobulins
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/74—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving hormones or other non-cytokine intercellular protein regulatory factors such as growth factors, including receptors to hormones and growth factors
    • G01N33/746—Erythropoetin
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2500/00—Screening for compounds of potential therapeutic value

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Pathology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Physics & Mathematics (AREA)
  • Endocrinology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)

Abstract

　被験物質のリガンドと該リガンドのレセプターの結合阻害活性の有無および／または強さを測定する方法であって、以下の（１）～（３）の工程を有する方法： （１）該被験物質又はリガンドのいずれかを固相化する工程 （２）該被験物質とリガンドを、該リガンドに対するレセプターの存在下又は非存在下で一定時間接触させる工程 （３）レセプターの存在下と非存在下における被験物質とリガンドの結合量を比較する工程。
PCT/JP2008/064091 2007-08-06 2008-08-06 リガンド－レセプター結合阻害活性の測定方法 WO2009020142A1 (ja)

Priority Applications (4)

Application Number Priority Date Filing Date Title
EP08792247.2A EP2187214B1 (en) 2007-08-06 2008-08-06 Method for measuring inhibitory activity on ligand-receptor binding
ES08792247.2T ES2550158T3 (es) 2007-08-06 2008-08-06 Método para medir la actividad inhibidora sobre la unión ligando-receptor
US12/672,237 US8796039B2 (en) 2007-08-06 2008-08-06 Method for measuring inhibitory activity on ligand-receptor binding
JP2009526470A JP5373610B2 (ja) 2007-08-06 2008-08-06 リガンド−レセプター結合阻害活性の測定方法

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2007-204303 2007-08-06
JP2007204303 2007-08-06

Publications (1)

Publication Number Publication Date
WO2009020142A1 true WO2009020142A1 (ja) 2009-02-12

Family

ID=40341374

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/JP2008/064091 WO2009020142A1 (ja) 2007-08-06 2008-08-06 リガンド－レセプター結合阻害活性の測定方法

Country Status (5)

Country Link
US (1) US8796039B2 (ja)
EP (1) EP2187214B1 (ja)
JP (1) JP5373610B2 (ja)
ES (1) ES2550158T3 (ja)
WO (1) WO2009020142A1 (ja)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2016536587A (ja) * 2013-10-31 2016-11-24 リジェネロン・ファーマシューティカルズ・インコーポレイテッド 中和抗体を検出するための競合リガンド結合アッセイ

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE102010029760B4 (de) * 2010-06-07 2019-02-21 Robert Bosch Gmbh Bauelement mit einer Durchkontaktierung und Verfahren zu seiner Herstellung
US9402890B2 (en) * 2011-02-01 2016-08-02 Uab Research Foundation Methods and compositions for pseudoinfectious alphaviruses
US8961995B2 (en) * 2012-09-20 2015-02-24 Uab Research Foundation Methods and compositions for alphavirus replicons

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH06501103A (ja) * 1991-06-20 1994-01-27 ベー・エル・アー・ハー・エム・エス・ディアグノスティカ　ゲーエムベーハー 生物学的液体中の抗体を決定する免疫学的分析方法とその方法を実施するキット
JPH09500215A (ja) * 1993-08-20 1997-01-07 ベー．アール．アー．ハー．エム．エス ディアグノス ティカ ゲーエムベーハー 被分析成分を測定する方法及び患者血清中の抗−ｔｓｈ受容体自己抗体の測定へのその使用
JP2003177131A (ja) * 2001-12-11 2003-06-27 Olympus Optical Co Ltd 生物学的な結合親和性を検出する方法

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH08228769A (ja) * 1994-12-27 1996-09-10 Tosoh Corp 組換えヒト甲状腺刺激ホルモンレセプターを発現するミエローマ細胞株
GB9909661D0 (en) 1998-06-06 1999-06-23 Rsr Ltd Assays for TSH receptor autoantibodies
DE10113042B4 (de) * 2001-03-09 2006-12-21 Bernhard-Nocht-Institut für Tropenmedizin HIV-Inhibitor Screening-System
WO2003018632A2 (en) * 2001-08-23 2003-03-06 Rsr Limited Epitope regions of a thyrotrophin (tsh) receptor, uses thereof and antibodies thereto

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH06501103A (ja) * 1991-06-20 1994-01-27 ベー・エル・アー・ハー・エム・エス・ディアグノスティカ　ゲーエムベーハー 生物学的液体中の抗体を決定する免疫学的分析方法とその方法を実施するキット
JPH09500215A (ja) * 1993-08-20 1997-01-07 ベー．アール．アー．ハー．エム．エス ディアグノス ティカ ゲーエムベーハー 被分析成分を測定する方法及び患者血清中の抗−ｔｓｈ受容体自己抗体の測定へのその使用
JP2003177131A (ja) * 2001-12-11 2003-06-27 Olympus Optical Co Ltd 生物学的な結合親和性を検出する方法

Non-Patent Citations (6)

* Cited by examiner, † Cited by third party
Title
JOURNAL OF IMMUNOLOGICAL METHODS, vol. 283, 2003, pages 317 - 329
JOURNAL OF IMMUNOLOGICAL METHODS, vol. 300, 2005, pages 179 - 191
N ENGL J MED., vol. 346, no. 7, 14 February 2002 (2002-02-14), pages 469 - 75
NEPHROL DIAL TRANSPLANT, vol. 20, no. 4, 2005, pages 16 - 22
See also references of EP2187214A4 *
YU. Y. ET AL.: "A novel method for detecting neutralizing antibodies against therapeutic proteins by measuring gene expression", J.IMMUNOL.METHODS, vol. 316, 2006, pages 8 - 17, XP028017630 *

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2016536587A (ja) * 2013-10-31 2016-11-24 リジェネロン・ファーマシューティカルズ・インコーポレイテッド 中和抗体を検出するための競合リガンド結合アッセイ
US10261094B2 (en) 2013-10-31 2019-04-16 Regeneron Pharmaceuticals, Inc. Competitive ligand binding assay for detecting neutralizing antibodies

Also Published As

Publication number Publication date
US20110207233A1 (en) 2011-08-25
EP2187214A1 (en) 2010-05-19
EP2187214A4 (en) 2010-08-11
EP2187214B1 (en) 2015-10-07
ES2550158T3 (es) 2015-11-04
JP5373610B2 (ja) 2013-12-18
US8796039B2 (en) 2014-08-05
JPWO2009020142A1 (ja) 2010-11-04

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