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WO2009011259A1 - 新規ｄｎａ断片およびそれを含む組換えベクター、それらによって形質転換された形質転換体、ならびにそれらの利用 - Google Patents

新規ｄｎａ断片およびそれを含む組換えベクター、それらによって形質転換された形質転換体、ならびにそれらの利用 Download PDF

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Publication number
WO2009011259A1
WO2009011259A1 PCT/JP2008/062392 JP2008062392W WO2009011259A1 WO 2009011259 A1 WO2009011259 A1 WO 2009011259A1 JP 2008062392 W JP2008062392 W JP 2008062392W WO 2009011259 A1 WO2009011259 A1 WO 2009011259A1
Authority
WO
WIPO (PCT)
Prior art keywords
same
dna fragment
recombinant vector
utilization
vector containing
Prior art date
Application number
PCT/JP2008/062392
Other languages
English (en)
French (fr)
Inventor
Yuji Hatada
Yukari Ohta
Yuko Hidaka
Nobuyuki Nakamura
Original Assignee
Japan Agency For Marine-Earth Science And Technology
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Japan Agency For Marine-Earth Science And Technology filed Critical Japan Agency For Marine-Earth Science And Technology
Priority to US12/452,648 priority Critical patent/US8354248B2/en
Priority to EP08778001.1A priority patent/EP2189531B1/en
Priority to DK08778001.1T priority patent/DK2189531T3/da
Priority to CN2008800245124A priority patent/CN101827937B/zh
Publication of WO2009011259A1 publication Critical patent/WO2009011259A1/ja

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/75—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Bacillus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
    • C12N9/2437—Cellulases (3.2.1.4; 3.2.1.74; 3.2.1.91; 3.2.1.150)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2468—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1) acting on beta-galactose-glycoside bonds, e.g. carrageenases (3.2.1.83; 3.2.1.157); beta-agarase (3.2.1.81)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01004—Cellulase (3.2.1.4), i.e. endo-1,4-beta-glucanase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01081—Beta-agarase (3.2.1.81)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

　本発明は、特定の遺伝子調節領域を単独、または該遺伝子調節領域およびシグナルペプチドをコードする遺伝子を含むＤＮＡ断片、該ＤＮＡ断片を含む組換えベクター、該組換えベクターを含む形質転換体、並びに該形質転換体を用いて組換えタンパク質を製造する方法に関する。本発明によれば、組換えタンパク質の種類に関係なく、タンパク質を大量かつ効率よく生産することができる。
PCT/JP2008/062392 2007-07-13 2008-07-09 新規ｄｎａ断片およびそれを含む組換えベクター、それらによって形質転換された形質転換体、ならびにそれらの利用 WO2009011259A1 (ja)

Priority Applications (4)

Application Number Priority Date Filing Date Title
US12/452,648 US8354248B2 (en) 2007-07-13 2008-07-09 Promoter-encoding DNA fragment, recombinant vector, recombiant transformant, and uses thereof
EP08778001.1A EP2189531B1 (en) 2007-07-13 2008-07-09 Novel dna fragment, recombinant vector containing the same, transformant transformed therewith and utilization of the same
DK08778001.1T DK2189531T3 (da) 2007-07-13 2008-07-09 Nyt DNA-fragment, rekombinant vektor indeholdende dette, transformant transformeret dermed samt dennes anvendelse
CN2008800245124A CN101827937B (zh) 2007-07-13 2008-07-09 新型dna片段与含有该片段的重组载体、利用它们转化得到的转化体以及它们的应用

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2007-183934 2007-07-13
JP2007183934A JP5126879B2 (ja) 2007-07-13 2007-07-13 新規ｄｎａ断片およびそれを含む組換えベクター、それらによって形質転換された形質転換体、ならびにそれらの利用

Publications (1)

Publication Number Publication Date
WO2009011259A1 true WO2009011259A1 (ja) 2009-01-22

Family

ID=40259596

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/JP2008/062392 WO2009011259A1 (ja) 2007-07-13 2008-07-09 新規ｄｎａ断片およびそれを含む組換えベクター、それらによって形質転換された形質転換体、ならびにそれらの利用

Country Status (6)

Country Link
US (1) US8354248B2 (ja)
EP (1) EP2189531B1 (ja)
JP (1) JP5126879B2 (ja)
CN (2) CN103710343B (ja)
DK (1) DK2189531T3 (ja)
WO (1) WO2009011259A1 (ja)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104388411B (zh) * 2014-12-03 2017-02-22 福州大学 一种琼胶酶及其基因和应用
WO2017038698A1 (ja) * 2015-08-28 2017-03-09 京セラ株式会社 光電変換装置
CN107354167A (zh) * 2017-07-11 2017-11-17 北京工业大学 高效降解油脂功能基因载体及其构建和应用
JP6650546B1 (ja) 2019-08-01 2020-02-19 日本食品化工株式会社 α−１，６−グルコシル転移反応を触媒する活性を有するタンパク質
JP6959411B1 (ja) 2020-07-31 2021-11-02 日本食品化工株式会社 糖のエピメリ化反応触媒用の酵素剤、エピメリ化反応生成物の製造方法およびエピメリ化反応生成物
CN114480207B (zh) * 2022-02-22 2023-09-22 青岛蔚蓝赛德生物科技有限公司 一种太平洋芽孢杆菌及其降解污废水中硫化物的应用

Citations (7)

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Publication number Priority date Publication date Assignee Title
EP0441361A2 (en) 1990-02-09 1991-08-14 Yamasa Shoyu Kabushiki Kaisha Process for producing foreign protein in escherichia coli
JPH05184372A (ja) 1991-10-04 1993-07-27 Nippon Suisan Kaisha Ltd 枯草菌によるストレプトリジンｏの製法及びそのための遺伝子組換え体
JPH06217781A (ja) * 1993-01-22 1994-08-09 Kao Corp 新規ｄｎａ断片、これを保有するプラスミドベクター及び組換え微生物並びにこれを用いる蛋白質の製造法
JP2000152784A (ja) * 1998-11-18 2000-06-06 Kao Corp タンパク質の製造法
JP2003000283A (ja) * 1998-03-31 2003-01-07 Itoham Foods Inc 発現ベクターの構築方法及びそれを介した天然型外来ポリペプチドの製造方法
JP2003052389A (ja) * 2001-06-06 2003-02-25 Takeda Chem Ind Ltd 新規プロモーター
JP2007183934A (ja) 2005-12-12 2007-07-19 Filippos Nikiforou メッセージシステムにおけるメッセージの自動インキュベーションおよび回復

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EP0151760A1 (en) * 1984-01-06 1985-08-21 Takeda Chemical Industries, Ltd. Novel DNA and use thereof
US4977089A (en) * 1987-01-30 1990-12-11 Eli Lilly And Company Vector comprising signal peptide-encoding DNA for use in Bacillus and other microorganisms
GB9006400D0 (en) * 1990-03-22 1990-05-23 Ciba Geigy Ag Bacterial vectors
US6506595B2 (en) * 1998-03-31 2003-01-14 Itoham Foods Inc. DNAs encoding new fusion proteins and processes for preparing useful polypeptides through expression of the DNAs
AU2001241125A1 (en) 2000-03-14 2001-09-24 Itoham Foods Inc. Process for producing polypeptide having disulfide bond
EP1400593A4 (en) * 2001-06-06 2005-04-06 Takeda Pharmaceutical NEW PROMOTER

Patent Citations (9)

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Publication number Priority date Publication date Assignee Title
EP0441361A2 (en) 1990-02-09 1991-08-14 Yamasa Shoyu Kabushiki Kaisha Process for producing foreign protein in escherichia coli
US5304471A (en) 1990-02-09 1994-04-19 Yamasa Shoyu Kabushiki Kaisha Process for producing foreign protein in Escherichia coli
JP2988951B2 (ja) 1990-02-09 1999-12-13 ヤマサ醤油株式会社 大腸菌における異種蛋白質の製造法
JPH05184372A (ja) 1991-10-04 1993-07-27 Nippon Suisan Kaisha Ltd 枯草菌によるストレプトリジンｏの製法及びそのための遺伝子組換え体
JPH06217781A (ja) * 1993-01-22 1994-08-09 Kao Corp 新規ｄｎａ断片、これを保有するプラスミドベクター及び組換え微生物並びにこれを用いる蛋白質の製造法
JP2003000283A (ja) * 1998-03-31 2003-01-07 Itoham Foods Inc 発現ベクターの構築方法及びそれを介した天然型外来ポリペプチドの製造方法
JP2000152784A (ja) * 1998-11-18 2000-06-06 Kao Corp タンパク質の製造法
JP2003052389A (ja) * 2001-06-06 2003-02-25 Takeda Chem Ind Ltd 新規プロモーター
JP2007183934A (ja) 2005-12-12 2007-07-19 Filippos Nikiforou メッセージシステムにおけるメッセージの自動インキュベーションおよび回復

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"Current Protocols in Molecular Biology", vol. 1-38, 1997, JOHN WILEY & SONS
"Molecular Cloning", 1989, COLD SPRING HARBOR LAB.
"Molecular Cloning: A Laboratory Manual", 1989, COLD SPRING HARBOR LABORATORY, COLD SPRING HARBOR
FUKUMORI ET AL., INT J SYST EVOL MICROBIOL., vol. 55, 2005, pages 2309 - 15
HATADA, Y. ET AL.: "Hyperproduction and Application of r-Agarase to Enzymatic Enhancement of Antioxidant Activity of Porphyran.", J. AGRIC. FOOD CHEM., vol. 54, 2006, pages 9895 - 9900, XP002577070 *
J. GEN. MICROBIOL, vol. 132, 1986, pages 2329 - 2335
OHTA, Y. ET AL.: "Enzymatic properties and nucleotide and amino acid sequences of a thermostable beta-agarase from the novel marine isolate, JAMB-A94.", BIOSCI. BIOTECHNOL. BIOCHEM., vol. 68, no. 5, 2004, pages 1073 - 1081, XP008129951 *
See also references of EP2189531A4 *

Also Published As

Publication number Publication date
CN101827937B (zh) 2013-09-11
EP2189531A1 (en) 2010-05-26
US20110014677A1 (en) 2011-01-20
JP5126879B2 (ja) 2013-01-23
US8354248B2 (en) 2013-01-15
CN103710343A (zh) 2014-04-09
EP2189531B1 (en) 2014-08-20
EP2189531A4 (en) 2010-09-08
DK2189531T3 (da) 2014-09-01
CN101827937A (zh) 2010-09-08
JP2009017841A (ja) 2009-01-29
CN103710343B (zh) 2016-01-20

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