[go: up one dir, main page]
More Web Proxy on the site http://driver.im/

WO2009044085A3 - Oligonucleotides, utilisation, methode de detection et kit permettant de diganostiquer la presence du gene e1 du virus de chikungunya - Google Patents

Oligonucleotides, utilisation, methode de detection et kit permettant de diganostiquer la presence du gene e1 du virus de chikungunya Download PDF

Info

Publication number
WO2009044085A3
WO2009044085A3 PCT/FR2008/051625 FR2008051625W WO2009044085A3 WO 2009044085 A3 WO2009044085 A3 WO 2009044085A3 FR 2008051625 W FR2008051625 W FR 2008051625W WO 2009044085 A3 WO2009044085 A3 WO 2009044085A3
Authority
WO
WIPO (PCT)
Prior art keywords
oligonucleotides
detection
gene
chikungunya virus
diagnosing
Prior art date
Application number
PCT/FR2008/051625
Other languages
English (en)
Other versions
WO2009044085A2 (fr
Inventor
Jean-Noel Telles
Original Assignee
Biomerieux Sa
Jean-Noel Telles
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Biomerieux Sa, Jean-Noel Telles filed Critical Biomerieux Sa
Priority to EP08835332A priority Critical patent/EP2185731A2/fr
Priority to US12/673,688 priority patent/US20100216117A1/en
Publication of WO2009044085A2 publication Critical patent/WO2009044085A2/fr
Publication of WO2009044085A3 publication Critical patent/WO2009044085A3/fr

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • C12Q1/701—Specific hybridization probes
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
    • Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Virology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

La présente invention concerne des oligonucléotides destinés à permettre l'amplification et la détection d'une séquence cible localisée dans le gène El du virus du Chikungunya. Ces oligonucléotides ont une longueur comprise entre 10 et 50 nucléotides et comprennent au moins un fragment de 10 nucléotides consécutifs issus des séquences suivantes : SEQ ID No. 1 : 5 ' -CTCTTACCGGGTTTGTTGC-3 '; SEQ ID No. 2 : 5 ' -GCCTGGACACCTTTCGAC-3 ' ou leur séquence complémentaire. L'invention concerne également l'oligonucléotide permettant la détection des amplicons, l'utilisation de ces oligonucléotides, une méthode de détection et un kit permettant de diagnostiquer la présence du gène El du virus du Chikungunya. L'invention trouve une application préférentielle dans le domaine du diagnostic.
PCT/FR2008/051625 2007-09-14 2008-09-11 Oligonucleotides, utilisation, methode de detection et kit permettant de diganostiquer la presence du gene e1 du virus de chikungunya WO2009044085A2 (fr)

Priority Applications (2)

Application Number Priority Date Filing Date Title
EP08835332A EP2185731A2 (fr) 2007-09-14 2008-09-11 Oligonucleotides, utilisation, methode de detection et kit permettant de diganostiquer la presence du gene e1 du virus de chikungunya
US12/673,688 US20100216117A1 (en) 2007-09-14 2008-09-11 Oligonucleotides, use, method of detection and kit for diagnosing the presence of the chikungunya virus e1 gene

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
FR0757598A FR2921064A1 (fr) 2007-09-14 2007-09-14 Oligonucleotides, utilisation, methode de detection et kit permettant de diagnostiquer la presence du gene e1 du virus du chikungunya
FR0757598 2007-09-14

Publications (2)

Publication Number Publication Date
WO2009044085A2 WO2009044085A2 (fr) 2009-04-09
WO2009044085A3 true WO2009044085A3 (fr) 2009-05-28

Family

ID=39099895

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/FR2008/051625 WO2009044085A2 (fr) 2007-09-14 2008-09-11 Oligonucleotides, utilisation, methode de detection et kit permettant de diganostiquer la presence du gene e1 du virus de chikungunya

Country Status (4)

Country Link
US (1) US20100216117A1 (fr)
EP (1) EP2185731A2 (fr)
FR (1) FR2921064A1 (fr)
WO (1) WO2009044085A2 (fr)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3392351A1 (fr) * 2008-04-21 2018-10-24 Gen-Probe Incorporated Procédé de détection de virus du chikungunya
WO2010071610A1 (fr) * 2008-12-19 2010-06-24 Agency For Science, Technology And Research (A*Star) Marqueurs biologiques de chikungunya sévère
MX2011008970A (es) * 2009-02-25 2012-03-06 Bigtec Private Ltd Sondas e iniciadores para la deteccion de chikungunya.
CN101935715B (zh) * 2010-09-08 2012-02-22 中国检验检疫科学研究院 实时荧光定量pcr检测基孔肯亚病毒核酸的方法
CN110607401A (zh) * 2019-10-10 2019-12-24 中国检验检疫科学研究院 一种快速检测基孔肯雅病毒的试剂盒
BR102020016662A2 (pt) * 2020-08-14 2022-03-03 Fundação Oswaldo Cruz Dispositivo portátil, método de leitura para detecção molecular automatizada de patógenos por lamp, controlado por aplicativo de smartphone e seus usos

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2005026388A2 (fr) * 2003-09-15 2005-03-24 Cenix Bioscience Gmbh Utilisation de genes eucaryotes agissant sur la separation de la chromatine pour diagnostiquer et traiter des maladies proliferatives
WO2007105111A2 (fr) * 2006-03-15 2007-09-20 Institut Pasteur Nouvelles souches isolées et purifiées du virus du chikungunya et leurs polynucléotides et séquences polypeptidiques, et leurs applications diagnostiques et immunogéniques

Family Cites Families (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4672040A (en) * 1983-05-12 1987-06-09 Advanced Magnetics, Inc. Magnetic particles for use in separations
US4683195A (en) * 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US4683202A (en) * 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US4800159A (en) * 1986-02-07 1989-01-24 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences
US5750338A (en) * 1986-10-23 1998-05-12 Amoco Corporation Target and background capture methods with amplification for affinity assays
US5234809A (en) * 1989-03-23 1993-08-10 Akzo N.V. Process for isolating nucleic acid
CA2020958C (fr) * 1989-07-11 2005-01-11 Daniel L. Kacian Methodes d'amplification de sequences d'acide nucleique
ZA936015B (en) * 1992-08-24 1994-03-10 Akzo Nv Elimination of false negatives in nuleic acid detection.
FR2749082B1 (fr) * 1996-05-24 1998-06-26 Bio Merieux Particules superparamagnetiques et monodispersees
FR2768743B1 (fr) * 1997-09-23 2001-09-14 Bio Merieux Procede de lyse de micro-organisme
FR2773416B1 (fr) * 1998-01-06 2000-02-11 Bio Merieux Particules magnetiques perfectionnees, leurs procedes d'obtention et leurs utilisations dans la separation de molecules
FR2781500B1 (fr) * 1998-07-23 2000-09-08 Bio Merieux Dispositif perfectionne et procede de lyse de micro-organismes
US6235502B1 (en) * 1998-09-18 2001-05-22 Molecular Staging Inc. Methods for selectively isolating DNA using rolling circle amplification
FR2791697B1 (fr) * 1999-04-01 2003-02-28 Biomerieux Sa Appareil et procede de lyse par ultrasons de cellules biologiques

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2005026388A2 (fr) * 2003-09-15 2005-03-24 Cenix Bioscience Gmbh Utilisation de genes eucaryotes agissant sur la separation de la chromatine pour diagnostiquer et traiter des maladies proliferatives
WO2007105111A2 (fr) * 2006-03-15 2007-09-20 Institut Pasteur Nouvelles souches isolées et purifiées du virus du chikungunya et leurs polynucléotides et séquences polypeptidiques, et leurs applications diagnostiques et immunogéniques

Non-Patent Citations (8)

* Cited by examiner, † Cited by third party
Title
DATABASE GENBANK 14 January 2003 (2003-01-14), KHAN A.H ET AL: "Chikungunya virus strain S27 prototype, complete genome", XP002458292, accession no. AF369024 *
EDWARDS ET AL: "Molecular diagnosis and analysis of Chikungunya virus", JOURNAL OF CLINICAL VIROLOGY, ELSEVIER, AMSTERDAM,, NL, vol. 39, no. 4, 19 July 2007 (2007-07-19), pages 271 - 275, XP022164805, ISSN: 1386-6532 *
HASEBE F ET AL: "Combined detection and genotyping of Chikungunya virus by a specific reverse transcription-polymerase chain reaction", JOURNAL OF MEDICAL VIROLOGY, vol. 67, no. 3, July 2002 (2002-07-01), pages 370 - 374, XP002470888, ISSN: 0146-6615 *
KHAN AFJAL HOSSAIN ET AL: "Complete nucleotide sequence of chikungunya virus and evidence for an internal polyadenylation site", JOURNAL OF GENERAL VIROLOGY, SOCIETY FOR GENERAL MICROBIOLOGY, SPENCERS WOOD, GB, vol. 83, no. Pt 12, December 2002 (2002-12-01), pages 3075 - 3084, XP002458289, ISSN: 0022-1317 *
PARIDA M M ET AL: "Rapid and real-time detection of Chikungunya virus by reverse transcription loop-mediated isothermal amplification assay.", JOURNAL OF CLINICAL MICROBIOLOGY FEB 2007, vol. 45, no. 2, February 2007 (2007-02-01), pages 351 - 357, XP002521990, ISSN: 0095-1137 *
PASTORINO B ET AL: "Development of a TaqMan RT-PCR assay without RNA extraction step for the detection and quantification of African Chikungunya viruses", JOURNAL OF VIROLOGICAL METHODS, AMSTERDAM, NL, vol. 124, no. 1-2, March 2005 (2005-03-01), pages 65 - 71, XP004714877, ISSN: 0166-0934 *
SANTHOSH ET AL: "Development and evaluation of SYBR Green I-based one-step real-time RT-PCR assay for detection and quantification of Chikungunya virus", JOURNAL OF CLINICAL VIROLOGY, ELSEVIER, AMSTERDAM,, NL, vol. 39, no. 3, 15 June 2007 (2007-06-15), pages 188 - 193, XP022118546, ISSN: 1386-6532 *
SMITS H L ET AL: "APPLICATION OF THE NASBA NUCLEIC ACID AMPLIFICATION METHOD FOR THE DETECTION OF HUMAN PAPILLOMAVIRUS TYPE 16 E6-E7 TRANSCRIPTS", JOURNAL OF VIROLOGICAL METHODS, vol. 54, no. 1, 1995, pages 75 - 81, XP009015131, ISSN: 0166-0934 *

Also Published As

Publication number Publication date
US20100216117A1 (en) 2010-08-26
FR2921064A1 (fr) 2009-03-20
EP2185731A2 (fr) 2010-05-19
WO2009044085A2 (fr) 2009-04-09

Similar Documents

Publication Publication Date Title
WO2001081622A3 (fr) Diagnostic de maladies associees a la reparation de l'adn
WO2001068912A3 (fr) Diagnostic d'affections associees a des genes suppresseurs de tumeurs et a des oncogenes
WO2002002807A3 (fr) Diagnostic des maladies associees a la signalisation cellulaire
WO2007087451A3 (fr) Compositions et procedes d’accroissement des interferences arn discriminatoires
WO2009115861A8 (fr) Typage et sous-typage moleculaire de salmonella par identification des sequences nucleotidiques variables des loci crispr
WO2009019657A3 (fr) Moyens de diagnostic et de traitement de la polyarthrite rhumatoïde
WO2012092569A3 (fr) Compositions contenant des acides nucléiques immunostimulants et méthodes associées
WO2009044085A3 (fr) Oligonucleotides, utilisation, methode de detection et kit permettant de diganostiquer la presence du gene e1 du virus de chikungunya
WO2007104318A3 (fr) Oligonucleotides comprenant des paires de signalisation et des nucleotides hydrophobes, balises sans tige, pour la detection d'acides nucleiques, de l'etat de methylation et de mutants d'acides nucleiques
WO2006106316A3 (fr) Amorces polynucleotidiques
PL2210954T3 (pl) Określanie stopnia metylacji DNA
EP2090652A4 (fr) Procede de detection du virus latent du houblon, ensemble d'amorces et necessaire destines a cette detection
WO2002046454A3 (fr) Diagnostic de pathologies associees a l'angiogenese
WO2011042901A8 (fr) Polynucléotides de bactériophage de brucella et utilisations correspondantes
WO2010034487A3 (fr) Moyen d’inhibition de l’expression d’orc-1
WO2007121946A3 (fr) Moyens pour inhiber l'expression de cd31
FR2902430B1 (fr) Oligonucleotides, utilisation, methode de detection et kit permettant de diagnostiquer la presence des genes h5 et n1 du virus d'influenza a
WO2005079173A3 (fr) Polynucleotide a polymorphisme a nucleotide unique associe a un cancer du colon, microreseau et trousse de diagnostic le comprenant, et methode de diagnostic d'un cancer du colon utilisant ledit polynucleotide
WO2007115344A8 (fr) Groupe d'oligonucléotides
WO2006123800A3 (fr) Element d'induction d'interference arn et son utilisation
WO2010131768A3 (fr) Marqueur d'adn unique à un plant d'erianthus spp.
MY175723A (en) Oligonucleotides and a method for detecting and/or quantifying human metapneumovirus (hmpv)

Legal Events

Date Code Title Description
121 Ep: the epo has been informed by wipo that ep was designated in this application

Ref document number: 08835332

Country of ref document: EP

Kind code of ref document: A2

WWE Wipo information: entry into national phase

Ref document number: 12673688

Country of ref document: US

WWE Wipo information: entry into national phase

Ref document number: 2008835332

Country of ref document: EP

NENP Non-entry into the national phase

Ref country code: DE