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WO2008139591A1 - Method of producing recombinant aprotinin - Google Patents

Method of producing recombinant aprotinin Download PDF

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Publication number
WO2008139591A1
WO2008139591A1 PCT/JP2007/059714 JP2007059714W WO2008139591A1 WO 2008139591 A1 WO2008139591 A1 WO 2008139591A1 JP 2007059714 W JP2007059714 W JP 2007059714W WO 2008139591 A1 WO2008139591 A1 WO 2008139591A1
Authority
WO
WIPO (PCT)
Prior art keywords
aprotinin
producing
recombinant aprotinin
yeast
recombinant
Prior art date
Application number
PCT/JP2007/059714
Other languages
French (fr)
Japanese (ja)
Inventor
Akihiro Meta
Hiroshi Nakatake
Takayuki Imamura
Chikateru Nozaki
Original Assignee
Juridical Foundation The Chemo-Sero-Therapeutic Research Institute
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Juridical Foundation The Chemo-Sero-Therapeutic Research Institute filed Critical Juridical Foundation The Chemo-Sero-Therapeutic Research Institute
Priority to PCT/JP2007/059714 priority Critical patent/WO2008139591A1/en
Publication of WO2008139591A1 publication Critical patent/WO2008139591A1/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/81—Protease inhibitors
    • C07K14/8107—Endopeptidase (E.C. 3.4.21-99) inhibitors
    • C07K14/811—Serine protease (E.C. 3.4.21) inhibitors
    • C07K14/8114—Kunitz type inhibitors
    • C07K14/8117—Bovine/basic pancreatic trypsin inhibitor (BPTI, aprotinin)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

It is intended to provide a yeast producing a recombinant aprotinin which has a native form N-terminal amino acid sequence and a method of preparing the same. Namely, an expression cassette for producing a recombinant aprotinin having native form N-terminal amino acid sequence which comprises a nucleic acid fragment having an α-mating factor signal sequence, an aprotinin structural gene and an ADH1 terminator attached to the downstream of a PRB1 promoter; a yeast producing a recombinant aprotinin in which the above-described expression cassette has been integrated; and a method of producing a recombinant aprotinin which comprises using the above-described yeast.
PCT/JP2007/059714 2007-05-11 2007-05-11 Method of producing recombinant aprotinin WO2008139591A1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
PCT/JP2007/059714 WO2008139591A1 (en) 2007-05-11 2007-05-11 Method of producing recombinant aprotinin

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/JP2007/059714 WO2008139591A1 (en) 2007-05-11 2007-05-11 Method of producing recombinant aprotinin

Publications (1)

Publication Number Publication Date
WO2008139591A1 true WO2008139591A1 (en) 2008-11-20

Family

ID=40001822

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/JP2007/059714 WO2008139591A1 (en) 2007-05-11 2007-05-11 Method of producing recombinant aprotinin

Country Status (1)

Country Link
WO (1) WO2008139591A1 (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2018110641A1 (en) * 2016-12-14 2018-06-21 国立大学法人山口大学 Method for overexpressing protein in yeast

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS59210888A (en) * 1983-02-22 1984-11-29 チロン・コ−ポレイシヨン Yeast developing vector and use thereof
JPH08163988A (en) * 1994-05-18 1996-06-25 Bayer Ag Method of preparing recombinant aprotinin having natural n-terminal sequence and variant of recombinant aprotinin
JP2007116983A (en) * 2005-10-27 2007-05-17 Chemo Sero Therapeut Res Inst Method for producing recombinant aprotinin

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS59210888A (en) * 1983-02-22 1984-11-29 チロン・コ−ポレイシヨン Yeast developing vector and use thereof
JPH08163988A (en) * 1994-05-18 1996-06-25 Bayer Ag Method of preparing recombinant aprotinin having natural n-terminal sequence and variant of recombinant aprotinin
JP2007116983A (en) * 2005-10-27 2007-05-17 Chemo Sero Therapeut Res Inst Method for producing recombinant aprotinin

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
FINNIS C. ET AL.: "Expression of recombinant platelet-derived endothelial cell growth factor in the yeast saccharomyces cerevisae", YEAST, vol. 8, 1992, pages 57 - 60, XP003024579 *
SLEEP D. ET AL.: "Saccharomyces cerevisiae strains that overexpress heterologous proteins", BIO./TECHNOLOGY, vol. 9, 1991, pages 183 - 187, XP002085129 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2018110641A1 (en) * 2016-12-14 2018-06-21 国立大学法人山口大学 Method for overexpressing protein in yeast

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