WO2005074961A1

WO2005074961A1 - Body fat-controlling agent

Info

Publication number: WO2005074961A1
Application number: PCT/JP2005/001883
Authority: WO
Inventors: Toshiaki Waga; Yoko Akazome; Koichi Nakazato; Kouzen Sou; Atsushi Inoue; Yasuhiro Mizushima
Original assignee: Asahi Breweries, Ltd.
Priority date: 2004-02-10
Filing date: 2005-02-09
Publication date: 2005-08-18
Also published as: JPWO2005074961A1

Abstract

It is intended to provide a body fat-controlling agent obtained from hop, i.e., a body fat-controlling agent originating in a natural material which has effects of reducing body fat and inhibiting body fat accumulation without lowering the skeletal muscle amount or the inner organ weight. More specifically speaking, it is intended to obtain polyphenol from hop and utilize the same in a body fat-controlling agent, a muscular tension-elevating agent or a drink, a food and a drug for reducing body fat, inhibiting body fat accumulation and elevating muscular tension. Namely, a body fat-controlling agent containing, as the active ingredient, polyphenol which is efficacious in reducing body fat and/or inhibiting body fat accumulation and a drink, a food and a drug containing the same.

Description

Specification

Body fat regulator

Technical field

The present invention relates to a body fat regulator obtained from hops.

Background art

[0002] In recent years, the dietary habits of Japanese people have become remarkably westernized, and their calories are increasing. In particular, excessive intake of lipids has resulted in excessive accumulation of fats, causing obesity. Obesity is considered to be a risk factor for ischemic heart disease, stroke, diabetes, etc. soon after being complicated by hypertension, impaired glucose tolerance, hyperlipidemia, etc.From the viewpoint of preventing lifestyle-related diseases, obesity countermeasures are extremely important. is there. Moreover, it cannot be said that it is preferable from the viewpoint of beauty.

[0003] Most of the conventional methods for preventing and treating obesity are based on dietary restrictions and diet diets. However, dietary restrictions are accompanied by mental difficulties, and malnutrition can cause malnutrition. Or at risk of developing pathological symptoms such as anorexia nervosa. In addition, it is generally known that excessive diet and inhibition of digestive enzymes reduce the supply of nutrients to the body, and thus involve not only lean body fat but also a decrease in skeletal muscle mass. This decrease in skeletal muscle mass causes rebound after dieting. On the other hand, various drugs have been administered, but the side effects as well as the efficacy must be considered. As described above, the prevention and improvement of obesity in the past were not easy and simple. In particular, due to the recent trend of health consciousness, natural sources that reduce only body fat without decreasing skeletal muscle mass have been awaited.

[0004] On the other hand, hop (Humulus Lupulus) is a perennial plant of the dioecious and dioecious plant belonging to the family Araceae. Unfertilized hop female flowers (cones) are widely used throughout the world as a raw material for beer. Hop flowers have yellow lupulin granules, which contain the resin that causes the bitterness of beer and the essential oils that give the aroma. Hops have an effect of imparting bitterness and aroma to beer, improving foam retention, and suppressing the growth of various bacteria, but are known to have many other medicinal effects. For example, it has been reported that it has allergic disease control, cancer prevention, anti-osteoporosis effect, diabetes complication prevention, antidepressant effect, etc. It is. In addition, as an effect of preventing and improving obesity, the present applicant has reported that hop has an effect of suppressing weight gain in mice (Patent Document 1). It also reports that it has an inhibitory activity on lipase, an enzyme that degrades lipids, as an inhibitor of hop digestive enzymes (Patent Document 2).

Patent Document 1: Japanese Patent Application Laid-Open No. 2001-131080

Patent Document 2: Japanese Patent Application Laid-Open No. 2001-321166

Disclosure of the invention

Problems to be solved by the invention

[0005] However, the effect of the hops described in Patent Document 1 on suppressing weight gain in mice is not always limited to the reduction of body fat alone, but to the reduction of skeletal muscle mass and visceral weight. I cannot deny that I have lost more weight. In addition, the inhibitory activity of lipase, which is a lipid-degrading enzyme described in Patent Document 2, suppresses the absorption of lipid components in foods into the body, but the body fat is not a carbohydrate protein that can be separated from lipids alone. For example, lipase inhibition does not necessarily reduce body fat because it is easily biosynthesized. Therefore, it has not been reported that the polyphenol component contained in hops has the effect of reducing body fat and the effect of suppressing the accumulation of Z or body fat without reducing skeletal muscle mass and visceral weight. .

[0006] An object of the present invention is to develop a body fat regulator having an action of reducing body fat and an action of suppressing the accumulation of Z or body fat, without reducing skeletal muscle mass and visceral weight! In particular, to provide a body fat regulator derived from natural products.

Means for solving the problem

[0007] The inventors of the present invention have conducted intensive studies on the above-mentioned problems, and as a result, from many natural products, polyphenols derived from hops, which are the raw materials for beer, have a structure that does not reduce the amount of skeletal muscle and visceral weight. They have found that they have an effect of suppressing fat reduction and accumulation, and based on this finding, they have completed the present invention.

[0008] That is, the first aspect of the present invention relates to a body fat regulator comprising a hop-derived polyphenol as an active ingredient, which is effective for reducing body fat and suppressing Z or accumulation.

[0009] A second aspect of the present invention is that the hop-derived polyphenol has a high content of procyan-gin. The present invention relates to the body fat regulator according to the first aspect.

[0010] A third aspect of the present invention relates to the body fat adjustor according to the second aspect, wherein the procyazine is a product obtained by polymerizing a catechin body.

[0011] A fourth aspect of the present invention relates to the body fat regulator according to any one of the first to third aspects, wherein the body fat is a visceral fat.

A fifth aspect of the present invention relates to the body fat regulator according to any one of the first to third aspects, wherein the body fat is subcutaneous fat.

[0013] A sixth aspect of the present invention relates to the body fat regulator according to any one of the first to third aspects, wherein the body fat is full fat.

[0014] A seventh aspect of the present invention relates to a food or drink containing the body fat regulator according to any one of the first to sixth aspects.

An eighth aspect of the present invention relates to a medicine containing the body fat regulator according to any one of the first to sixth aspects.

A ninth aspect of the present invention relates to a method for using a hop-derived polyphenol that is effective for reducing body fat and suppressing Z or accumulation for the production of a body fat regulator.

[0017] The hop-derived polyphenol (hereinafter referred to as "hop polyphenol") in the present invention is, for example, extracted from hops with alcohol or the like, then clarified and passed through a styrenedibutylbenzene-based synthetic adsorption resin. It is obtained by adsorbing the polyphenol component by liquefaction, washing with water to completely remove saccharides and organic acids, and eluting with aqueous ethanol. The hop polyphenol obtained in this way has an effect of reducing body fat and suppressing Z or accumulation, and thus has an effect of preventing diseases caused by obesity, maintaining health, etc. It is added to food and drink as a functional food or formulated as a pharmaceutical. It also has the function of enhancing muscle and muscle tension, preventing muscle attenuation and increasing muscle and muscle tension.

As the hop polyphenol component, flavonoid glycosides such as high molecular weight procyan-dine, rutin, and isoquercitrin polymerized with a catechin body occupy most.

[0019] In the present invention, the "body fat regulator" refers to a substance that suppresses the accumulation of energy as body fat caused by excessive energy absorption, and uses this energy for activities such as muscles and internal organs. It refers to the action of converting into energy and reducing excess body fat, which regulates the amount of body fat and suppresses the increase. Therefore, the adjustment of body fat prevents unnecessary accumulation of body fat, which does not mean a dangerous action such as suppressing energy absorption more than necessary or reducing body fat and making it lean. This is because, as shown in the Examples below, when the group to which the body fat regulator of the present invention was administered was bred on a high-nutrition diet, the average body weight was not different from that of the non-administration group, but the body fat as a body fat was not changed. This is evident from the fact that fat is reduced, while skeletal muscle mass and visceral weight are not reduced. Therefore, the body fat regulator of the present invention suppresses fat accumulation and acts to prevent obesity when administered in a growing condition, and when administered in a condition that is already obese, produces fat muscle and the like. Promotes conversion to protein and works to improve obesity.

[0020] The term "muscle tension enhancement" refers to an increase in muscle mass or an increase in muscle tension (force exerted by muscle). "Muscle attenuation" refers to a decrease in muscle mass or a decrease in muscle tension, and includes muscle atrophy, muscle loss, and muscle fatigue.

[0021] The body fat regulator of the present invention can be made into a formulation together with commonly used carriers, auxiliaries, additives, and the like, and can be used as a pharmaceutical in a product such as an oral product according to a conventional method. Or mixed with food materials to produce food and drink.

[0022] In addition, administration as a medicament reduces body fat and eliminates obesity. Ingestion as a food or drink can be used as a health food or functional food to prevent or maintain health due to obesity. Used.

The invention's effect

[0023] Since the body fat regulator of the present invention contains hop polyphenol, which is derived from natural products, as an active ingredient, it has low side effects and is highly safe for the living body. In addition, it does not reduce skeletal muscle mass and visceral weight and is excellent in the action of reducing excess body fat and suppressing the accumulation of excess energy in the form of body fat. Products or pharmaceuticals have high safety and are very useful for preventing and improving obesity. Brief Description of Drawings

FIG. 1 is a diagram showing an increase in volume during a breeding period.

FIG. 2 is a view showing an average food consumption per day. BEST MODE FOR CARRYING OUT THE INVENTION

[0025] The hop which is a raw material of the present invention is a perennial plant of dioecious and dioecious species belonging to the family Araceae which is widely used as a raw material for beer throughout the world, and its unfertilized female flower has matured. It is a cone. This hop is used in the form of hop bracts, which are the source of bitterness of hop cones or beer. There is no problem using raw materials such as

Hop Power As a method for extracting polyphenol, a raw material such as hop bracts or hop cones or hop pellets containing hop bracts is mixed with water or a water such as alcohol, acetone, acetonitrile or the like of 50 v / v% or less. Extract with an aqueous solution of an organic solvent that is miscible with water. A preferred example is water or ethanol containing 50 vZv% or less of water-containing ethanol. The ratio between the raw material and the extraction solvent is preferably about 1:20 to 100 (weight ratio). The extraction is preferably performed at about 195 ° C. under stirring for about 20 to 60 minutes. An extract is obtained by filtration. If necessary, a filter aid such as perlite can be used.

[0027] The extract thus obtained is treated with a gel-type synthetic adsorbent and Z or an ultrafiltration membrane.

[0028] The treatment with the gel-type synthetic adsorbent will be described. With respect to the extract of the raw material obtained in the above-mentioned extraction step, the gel-type synthetic adsorbent is adsorbed on the gel-type synthetic adsorbent, and the gel-type synthetic adsorbent is adsorbed with an aqueous solution of water or ethanol, preferably an ethanol solution of 11 OvZv%. A washing step of washing the adhesive and an elution step of eluting the adsorbed fraction from the gel-type synthetic adsorbent with an aqueous ethanol solution or ethanol of 30 vZv% or more to obtain a body fat regulator

[0029] The adsorption step is a step of cooling the same extraction solution to a room temperature of about 15 to 30 ° C, passing the solution through a column filled with a gel-type synthetic adsorbent, and allowing the adsorbent to adsorb the body fat regulator. It is. At that time, if necessary, in order to increase the adsorption efficiency, it is desirable to reduce the organic solvent concentration of the extract to 10% or less in advance by concentration under reduced pressure or the like. Examples of the material of the gel-type synthetic adsorbent include hydrophilic bur polymer, hydroxypropyl dextran, and styrylene benzene polymer. The liquid passing time is preferably set so that the SV value is between 0.5 and 100. The SV value referred to here is the following formula This is the value defined by 1.

[0030] [number 1]

3 value = [Pouring amount (L)] Z {[Resin amount (L)] X [Pouring time (h)]}

[0031] The washing step is a step of washing the gel-type synthetic adsorbent holding the body fat regulator, and by this step, it is possible to remove contaminants and to further improve the purification degree of the body fat regulator. . As a solvent used for washing, water or an aqueous solution of 110 to 110 vZv% of ethanol is preferable, and it is preferable to wash by passing a solvent amount of about 110 to 10 times the amount of the resin.

[0032] The elution step is a step of desorbing and eluting the body fat adjuster from the gel-type synthetic adsorbent holding the body fat adjuster, and using a solvent used for elution such as hydrous alcohol, hydrous acetone, or hydrous acetonitrile. Particularly preferred examples include 30 vZv% or more of an aqueous ethanol solution or ethanol. It is desirable that the flow rate of the elution solvent be about 116 times the amount of the resin.

[0033] The obtained eluate is concentrated under reduced pressure, and the solvent is removed by a usual method such as freeze-drying or spray-drying to obtain a body fat regulator as a powder. At the time of concentration under reduced pressure, a solvent such as alcohol, acetone, or acetonitrile can be recovered and reused. The body fat regulator obtained in this manner is an odorless flesh-colored, brown or pale yellow powder that has a slightly bitter taste, and the body fat regulator itself adsorbs to the gel-type synthetic adsorbent, and It is a polyphenol-like substance that does not permeate through an ultrafiltration membrane with a molecular weight cut-off of 1,000 or more.

[0034] The yield is 0.5-20. OwZw% in terms of hop bract weight, and 0.5% in terms of hop cone weight.

5— 15. OwZw%. The gel-type synthetic adsorbent used can be repeatedly used after washing with an aqueous alcohol solution of 80 vZv% or more, an aqueous solution of sodium hydroxide of about 0.05 N, or the like.

Next, the treatment by the ultrafiltration membrane will be described. The hop extract obtained in the above extraction step is treated with an ultrafiltration membrane having a molecular weight cut-off of 1,000 or more. If necessary, the extract may be concentrated under reduced pressure to reduce the concentration of the organic solvent. The recovered organic solvent can be reused. Materials for the membrane include cellulose, cellulose acetate, polysulfone, polypropylene, polyester, polyether sulfone, PVDF, etc. Any material can be used without particular limitation as long as it is usually used as a material for an ultrafiltration membrane. Also, if the molecular weight cut-off is 1,000 or more, it can be used without any problem.If a membrane with a high molecular weight cut-off is used, the yield will be extremely reduced. An ultrafiltration membrane having a molecular weight cut-off of 10,000 to 20,000 is suitable because the time required is long. In addition, the treatment is desirably performed until the amount of the remaining liquid is about lZio-lZioo at the start of the treatment, which is a force depending on the type of the extraction solvent and the ratio of the extraction solvent to the hop or hop bract. The pressure at that time depends on the ultrafiltration membrane and the filtration device, but is preferably about 0.1 to 10. Okg / cm ² . If necessary, once treated, dilute the remaining solution again with an appropriate solvent such as water, and re-process similarly to increase the degree of purification.

[0036] The solvent of the obtained upper remaining liquid is removed by a conventional method such as concentration, freeze-drying, or spray-drying to obtain a body fat regulator as a powder. Further, at the time of concentration under reduced pressure, a solvent such as alcohol, acetone, and acetonitrile can be recovered and reused. The body fat regulator obtained in this manner is an odorless flesh-colored, brown or pale yellow powder that has a slightly bitter taste, and the body fat regulator itself is adsorbed by the gel-type synthetic adsorbent, and It is a polyphenol-like substance that does not permeate through an ultrafiltration membrane with a molecular weight cut-off of 1,000 or more.

[0037] The yield is 0.5-20. OwZw% in terms of hop bract weight, and 0.5% in terms of hop cone weight.

5— 15. OwZw%.

[0038] Since the active bodies of the body fat regulator obtained by the treatment with the gel-type synthetic adsorbent or the treatment with the ultrafiltration membrane are the same polyphenols, they are obtained by the treatment with the gel-type synthetic adsorbent. The obtained body fat regulator can be dissolved in an appropriate solvent such as an aqueous alcohol solution and treated with an ultrafiltration membrane to further improve the degree of purification of the polyphenols as the active substance. The reverse is also possible. Needless to say, a sufficiently useful body fat regulator can be obtained by a method using a gel-type synthetic adsorbent or a method using an ultrafiltration membrane alone.

[0039] The composition of the hop polyphenol obtained in the present invention is mainly composed of a polymer procyazine which is obtained by polymerizing a force techin body as a polymer polyphenol conjugate, and in addition, rutin, It also contains flavonoid glycosides such as isoquercitrin, and these components are effective in reducing body fat and suppressing accumulation. Furthermore, it is also effective in enhancing muscle and muscle tension.

Hop Force The extracted polyphenol can be formulated together with commonly used carriers, auxiliaries, additives, etc., and can be made into an oral product according to a conventional method and used as a pharmaceutical. It can be mixed with ingredients to make food and drink.

[0041] Pharmaceuticals include tablets, capsules, granules, syrups and the like as oral preparations. When these products are administered to the human body as pharmaceuticals, the force varies depending on the type of preparation, processing conditions, symptoms of the recipient, physical condition, height, weight, etc. Preferably, 0.1-80 mgZkg body weight is administered once or several times a day to achieve its effect sufficiently.

[0042] Pharmaceuticals containing the body fat regulator of the present invention can be prepared by a usual method using an inert, non-toxic, pharmaceutically suitable excipient or solvent, and a usual formulation such as a tablet. , Capsules, dragees, pills, tablets, fine granules, aerosols, syrups, emulsions, suspensions and solutions. The therapeutically effective compound can be present in each case in a concentration of about 0.5 to 90% by weight, based on the total formulation, ie in an amount sufficient to achieve the above-mentioned effects. Combinations are prepared, for example, by extending the active compound with solvents and / or excipients, if appropriate with emulsifiers and Z or suspending agents. If water is used as diluent, organic solvents can be used as auxiliary solvents, if appropriate. Auxiliaries include, for example, water, non-toxic organic solvents such as paraffin (eg petroleum fractions), vegetable oils (eg peanut oil, sesame oil) and alcohols (eg ethanol and glycerin), excipients such as powders. Natural minerals (eg clay, alumina, talc and chalk), powdered synthetic minerals (eg highly dispersible silica and silicates), sugars (eg sucrose, ratatose and dextrose), emulsifiers (eg polyoxyethylene fatty acid esters and Polyoxyethylene fatty alcohol ethers, alkyl sulfonates, aryl sulfonates), suspending agents (eg, lignin sulfite waste liquor, methylcellulose, starch and polyvinylpyrrolidone) and lubricants (eg, magnesium stearate, talc, Stearic acid and sodium lauryl sulfate) It is below.

[0043] Administration is performed in a usual manner, preferably by a force used orally or parenterally. . In that special case, it can also be carried out lingually or intravenously. Injectable media is in particular water, which contains stabilizers and solubilizers customary for injection solutions and Z or buffers. Such additives include, for example, tartrate buffer, borate buffer, ethanol, dimethyl sulfoxide, complexing agents (eg, ethylenediaminetetraacetic acid), high molecular weight polymers for adjusting viscosity (eg, liquid polyethylene oxide). ) Or a polyethylene derivative of hydrogenated sorbitan. In the case of oral administration, particularly in the case of aqueous suspensions, flavoring agents or coloring agents can be added to the active conjugates together with the above-mentioned auxiliary agents.

[0044] The food and drink containing the body fat regulator of the present invention may be in the form of the above-mentioned preparation, but may be in any food form such as solid food, semi-liquid food, gel food, and beverage. For example, soft drinks, teas, coffee, soups, liqueurs, low-malt beer, milk, whey drinks, lactic acid drinks, jelly drinks, candy (candy), chewing gum, chocolate, gummy, using any commonly used base material , Yogurt, ice cream, rice crackers, cookies, etc. In the form of these foods and the like, the required amount can be added to each food raw material and processed and manufactured by a general manufacturing method. The preferred blending amount at this time is not particularly limited, but is 0.01 to 50% by weight, preferably 0.1 to 50% by weight in consideration of the characteristics, taste, intake, safety, economy, etc. of various foods and drinks. It is advisable to mix 1 to 10% by weight at the appropriate stage of the manufacturing process according to the purpose.

[0045] Foods and drinks containing the body fat regulator of the present invention are used for disease prevention, health maintenance, muscle strengthening, and the like, and the intake amount is not particularly limited, but is 0.1 per day. Ingested as processed goods containing 1,1Og, preferably 11Lg.

When adding a body fat adjuster to these foods and drinks, the body fat adjuster may be added in powder form, but preferably the body fat adjuster is added to a 12% aqueous solution or alcohol. An aqueous solution is preferably added as an alcohol solution.

[0047] In addition, the food or beverage containing the body fat accumulation inhibitor of the present invention may contain various components depending on the form of the food.

[0048] The various components referred to herein include starch, polysaccharides such as corn starch, dextrin, sucrose, glucose, fructose, maltose, stevioside, corn syrup, lactose, nicotinamide, calcium pantothenate, calcium salts, vitamins Group B, Aspar Tame, xylitol, sorbitol, sorbitan fatty acid ester, L-ascorbic acid, heart tocopherol, sodium erythorbic acid, citric acid, tartaric acid, malic acid, succinic acid, lactic acid, gum arabic, carrageenan, pectin, amino acids, yeast extract, glycerin Fatty acid esters, sucrose fatty acid esters, glycerin, propylene glycol, casein, gelatin, agar, pigments, fragrances, preservatives and the like.

Example

Hereinafter, the present invention will be described in more detail with reference to Examples, but the present invention is not limited to these Examples.

[Example 1] (Preparation of body fat regulator from hop cones using gel-type synthetic adsorbent)

20 g of hop cones were ground in a mortar and extracted with 2 L of water at 95 ° C for 40 minutes with stirring. After filtration, the mixture was allowed to cool, and the extract was passed through a column filled with 80 ml of hydrophilic BURU polymer resin (Toyopearl HW40, manufactured by Tosoichi Co., Ltd.) over 2 hours (SV = 12.5). It was washed with a 5% aqueous ethanol solution. Further, 400 ml of an 80% aqueous ethanol solution was passed through the column, and the eluate was collected and freeze-dried to obtain 800 mg of a body fat regulator as a pale yellow powder having an odorless and slightly bitter taste. Hop power yield was 4%.

[Example 2] (Preparation of body fat regulator from hop bract with gel-type synthetic adsorbent)

20 g of hop bracts were extracted with 600 ml of a 50% aqueous ethanol solution at 80 ° C. for 40 minutes with stirring. After filtration, the solution was concentrated under reduced pressure to a volume of 300 ml, and the concentrated solution was passed through a column filled with 80 ml of styrene dibutyl benzene resin (Sepabeads 825, manufactured by Mitsubishi Iridaku Co., Ltd.) over 1 hour (SV = 3.75), poured and washed with 400 ml of water. Further, 400 ml of an 80% aqueous ethanol solution was passed through the column, and the eluate was collected and freeze-dried to obtain 1.6 g of a body fat regulator as a pale yellow powder having an odorless, slightly bitter taste. Obtained. Hop bract yield was 8%.

[Example 3] (Preparation of body fat regulator from hop cone using ultrafiltration membrane)

20 g of hop cones were ground in a mortar and extracted with 2 L of water at 95 ° C for 40 minutes with stirring. After filtration, the mixture was allowed to cool, and the extract was treated with an ultrafiltration membrane (XM50, manufactured by Amicon) having a molecular weight cut off of 50,000 at 1.0 kg / cm ² at room temperature until the volume became 20 ml. The obtained upper residue was evaporated to dryness under reduced pressure to obtain 200 mg of a body fat regulator as a pale yellow powder having an odorless and slightly bitter taste. The yield from hops was 1%.

[Example 4] (Preparation of body fat regulator of hop bracts by ultrafiltration membrane)

20 g of hop bracts were extracted with 600 ml of a 50% aqueous ethanol solution at 80 ° C. for 40 minutes with stirring. After filtration, the extract was treated with an ultrafiltration membrane (YM10 manufactured by Amicon) having a cut-off molecular weight of 10,000 until it reached 60 ml at 3. Okg / cm ² at room temperature. The obtained upper residue was freeze-dried to obtain 0.8 g of a body fat regulator as a pale yellow powder having an odorless and slightly bitter taste. The yield from hop bracts was 4%.

[Test Example 1] (Effect of suppressing body fat accumulation (rat))

With respect to the body fat regulator obtained in Example 1, the inhibitory effect on body fat accumulation was examined using Wistar male rats (11 weeks old).

[0055] <Subject, feed, intake method>

36 male Wistar rats (11 weeks old) were preliminarily reared for 1 week, and rats without any abnormalities were divided into three groups so that the body weight would be the same between each group. 2% by weight of the body fat regulator obtained in Example 1 was added to an animal feed (manufactured by Oriental Yeast Co., Ltd.), and the well-kneaded feed was fed and fed freely for 3 weeks. Rats were weighed over time. In parallel with this, as a control group, rats in the second group were bred for 3 weeks with the above-mentioned diet for experimental animals only, and the body weight of the rats was similarly measured over time. The weight gain during the breeding period is shown in FIG. 1 in comparison with the control group. Figure 2 shows the daily food consumption of rats in each group during the breeding period. The feed composition of the experimental animal feed (manufactured by Oriental Yeast Co., Ltd.) was based on the standard purified feed AIN-93M published in the US National Nutrition Research Institute (AIN) shown in Table 1.

[Table 1] Feed (AIN—93 M) composition

After 3 weeks of breeding, the heart, liver, kidney, internal organs, soleus, soleus, plantaris, and gastrocnemius of the rats in the test and control groups were excised and weighed. It was measured. The results are shown in Table 2 in comparison with the control group. In addition, the tissue weight and visceral fat weight shown in Table 2 represent those corrected for the body weight after dissection.

[Table 2]

Tissue weight

*: P <0.05 V s Control outlet

[0059] <Result>

As a result, as shown in FIG. 1 and FIG. 2, there was no difference in body weight change and food consumption between the test group and the control group. In addition, each tissue weight corrected by post-dissection weight As shown in Table 2, the skeletal muscles of the lower limbs (the soleus, plantar, and gastrocnemius), liver, heart, and liver showed no difference in organ weight between the test group and the control group. Visceral fat content was reduced in the test group. This indicates that the body fat regulator containing hop polyphenols suppresses the accumulation of body fat without suppressing weight gain and without reducing skeletal muscle mass and organ weight. In other words, it can be seen that it has the effect of changing the form of energy storage to muscles other than fat.

[0060] [Example 5] (Tablets)

150 g of a body fat regulator of hop polyphenol obtained in Example 2, 90 g of ratatose and 17 g of corn starch were mixed, and the mixture was granulated together with a paste prepared from 70 g of corn starch. The obtained granules were mixed well with magnesium stearate lg, and the mixture was tableted with a tableting machine to produce 1,000 tablets.

[Comparative Example 1] (Tablet)

140 g of ratatose and 17 g of corn starch were mixed, and this mixture was granulated together with a paste prepared in advance from 70 g of corn starch. Magnesium stearate (lg) was added to the obtained granules, mixed well, and the mixture was tableted with a tableting machine to produce 1,000 tablets.

[Test Example 2] (Body fat reducing action of body fat regulator (human))

Next, the tablet obtained in Example 5 was ingested by humans and subjected to a clinical test to examine the body fat reducing effect of the body fat regulator extracted from hop bracts.

[0063] <Target>

In a preliminary study conducted four weeks prior to the start of the study, 50 male volunteers who were voluntary volunteers to participate in this study and who had a body mass index of 22 and a BMI ≤ 30 among paid volunteers Selected. However, those taking drugs or health foods that may affect lipid metabolism, those who have a history of food allergies, 200 ml within 1 month from the start of the study, or 400 ml within 3 months Those who collected blood (such as blood donation) that exceeded the threshold, and those judged by the investigator to be unsuitable for study participation were excluded from the study. Based on the results of the preliminary tests (blood and physical tests) and CT tests performed before ingestion, physicians who did not directly participate in the test Blood pressure, neutral Fat, total fat area (TFA), visceral fat area (VFA) and subcutaneous fat area (SFA), West Z hips, etc. were divided into two groups so as to have the same background. In addition, this study was conducted in accordance with the intent of the Declaration of Helsinki, with sufficient explanation of the research content and methods to the subjects, and with written consent.

[0064] <Tablet pill>

The ingestible tablet is a test tablet (150 mg, 1 tablet) containing the body fat adjuster obtained in Example 5 and the body fat adjuster obtained in Comparative Example 1! Pana!とした Control tablets (hereinafter referred to as placebo tablets). Before conducting the study, the investigator confirmed that the test tablet and the placebo tablet were indistinguishable due to sensory aspects such as flavor and aroma and packaging.

[0065] <Ingestion method and schedule>

The study was a parallel two-group comparative study employing a double-blind method by random assignment. The study period was set for a total of 20 weeks: 4 weeks of pre-observation, 12 weeks of ingestion, and 4 weeks of post-observation after ingestion. The test subjects were divided into two groups and consumed test tablets in the following manner.

Test group: Each test tablet (150 mgZ, 1 tablet) was ingested before dinner (4 tablets each, total 4 tablets Z days). Control group: Placebo tablets (150 mgZ, 1 tablet) were each taken before dinner (4 tablets each, 4 tablets Z days). The subjects were instructed not to change their daily life, such as eating habits, smoking and exercise, except taking test tablets or placebo tablets every day.

[0066] <CT examination>

All subjects underwent abdominal fat analysis by CT scan. The analysis was performed using an L4ZL5 abdominal CT tomography image of the intervertebral disc, and a total fat area (TFA) using the PC software "Fat Scan" (manufactured by N2 System Co., Ltd.). The visceral fat area (VFA) and subcutaneous fat area (SFA) were determined.

[0067] The test was conducted twice in total, from the start of ingestion and 12 weeks after ingestion (end of ingestion). In principle, the test was performed within 4 days before and after the test. On the test day, the test was performed after at least 4 hours of fasting and 2 hours of fasting.

[0068] In addition to the CT inspection, the waist was also measured. Measurement starts at the start of intake and intake 4 Weekly, 8 weeks after ingestion, 12 weeks after ingestion (end of ingestion), 5 times after the post-observation period.

[0069] <Result>

These results are shown in Table 3 in comparison with the control group. A two-way analysis of variance showed a significant difference in waist between the test group and the control group (ANOVA: waist p <0.05).

[0070] Further, in the transition of visceral fat area in all subjects, the test group showed a decreasing tendency 12 weeks after ingestion, while the control group showed an increasing tendency.

[0071] In the transition of the total fat area in all subjects, the test group showed a decreasing tendency 12 weeks after ingestion, while the control group showed an increasing tendency.

[0072] From the above results, it was confirmed that hop polyphenol can be expected to have a cosmetic effect by tightening the waist and has an effect of reducing body fat.

[0073] [Table 3]

Group Intake condition Item Ingestion start date Intake 4 weeks after ingestion 8 weeks after ingestion 1 week after ingestion After observation period Test tablet (Polywest size (cm) 85.7 ± 6.5 86.3 ± 6.3 85.5 ± 6.2 85.8 ± 6.1 85.0 ± 6.2 Phenol Contained

Waist size ((cm) 0.0 ± 0.0 0.6 ± 2.8 -0.1 ± 2.1 0.1 ± 2.2-1 0.7 ± 1.9 tablets) before dinner

4 tablets (total of 4 tablets) Visceral fat area (cm ² ) 90.3 ± 39.0 11 88.6 ± 41.1 ― Test group

/ Day) Ingestion l Visceral fat area (cm ² ) 0.0 ± 0.0 1-1.7 ± 11.7 1 Total fat area (cm ² ) 255.3 ± 74.5 ― 1 247.4 ± 82.6 ― Total fat area * ³ (cm ² ) 0.0 ± 0.0 One-7.9 ± 24.4 One control tablet (Blast width (cm) 85.7 ± 6.0 85.7 ± 6.2 86.9 ± 6.8 85.9 ± 6.5 85.7 ± 6.4 Sebo tablet)

^ West size "(cm) 0.0 ± 0.0 0.1 ± 1.2 1.3 ± 2.6 0.2 ± 1.8 0.0 ± 1.8 4 tablets before meals (total

4 tablets / day) Ingestion Visceral fat area (cm ² ) 93.1 ± 44.5 1 98.2 ± 41.0 1 Control group

Visceral fat area * ² (cm ² ) 0.0 ± 0.0 1 5.0 ± 18.7 ― Whole visceral fat area (cm ² ) 267.5 ± 76.5 ― 279.3 ± 74 ・ 7 1 l Total fat area * ³ (cm ² ) 0.0 ± 0.0 1 1 11.8 ± 30.6 1 Note 1) ρ <0.05 (ANOVA)

Note 2) ρ <0.1 (paired t-test)

* 1) Waist dimensions Waist dimensions after each intake period-West dimensions on the disclosure date of intake

* 2)] Visceral fat area = Visceral fat area after each intake period-Visceral fat area on the disclosure date of intake

* 3) ^ Total fat area = Total fat area after each intake period Total fat area on the disclosure date of intake

[Example 6] (Tablets, capsules)

55.5 g of body fat regulator obtained in Example 1, 41. Og of crystalline cellulose, 2. Og of fine-grained diacid, 2. Og, and 1.5 g of sucrose fatty acid ester Total 100. Og Each of the above parts by weight Were uniformly mixed to obtain tablets and capsules according to a conventional method.

[Example 7] (Powder, granule)

The body fat regulator obtained in Example 1 (20. Og), starch (30.0 g), and lactose (50. Og) totaled 100. Og. The above parts by weight were uniformly mixed to obtain powders and granules according to a conventional method.

[Example 8] (Beverage)

0.45 g of the body fat regulator obtained in Example 1, apple clear concentrated juice 15.0 g, fructose 5.0 g, citrate 0.2 g, flavor 2.0 g, pigment 0.15 g, sodium ascorbate 0. A beverage was produced with the composition of each of the above components in 05 g, 77.15 ml of water.

[Example 9] (candy)

20.0 g of sucrose, 70.0 g of starch syrup (75% solid content), 9.5 g of water, 0.45 g of coloring agent, 0.04 g of fragrance, 0.011 g of body fat regulator obtained in Example 1 in total 100 0.0g Each of the above parts by weight was used to make candy according to a conventional method.

[0078] [Example 10] (Cookie)

32.0 g of flour, 16.0 g of whole egg, 19.0 g of knotter, 25.0 g of sugar, 7.2 g of water, 0.2 g of baking powder, 0.6 g of body fat regulator obtained in Example 1 in total of 100. 0 g Each of the above parts by weight of each component was used as a cookie according to a conventional method.

Claims

The scope of the claims

[1] A body fat regulator comprising hop-derived polyphenol as an active ingredient, which is effective in reducing body fat and suppressing Z or accumulation.

[2] The body fat regulator according to claim 1, wherein the hop-derived polyphenol has a high content of procyanidin.

[3] The body fat regulator according to claim 2, wherein the procyazine is a polymerized catechin body.

4. The body fat regulator according to claim 1, wherein the body fat is visceral fat.

5. The body fat regulator according to claim 1, wherein the body fat is subcutaneous fat.

6. The body fat regulator according to claim 1, wherein the body fat is a total fat.

[7] A food or drink containing the body fat regulator according to any one of claims 1 to 6.

[8] A drug containing the body fat regulator according to any one of claims 1 to 6.

[9] A method of using a hop-derived polyphenol that is effective in reducing body fat and / or suppressing accumulation for the production of a body fat regulator.