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WO2004013171A3 - Multi-subunit protein production system in transgenic insect - Google Patents

Multi-subunit protein production system in transgenic insect Download PDF

Info

Publication number
WO2004013171A3
WO2004013171A3 PCT/GB2003/003319 GB0303319W WO2004013171A3 WO 2004013171 A3 WO2004013171 A3 WO 2004013171A3 GB 0303319 W GB0303319 W GB 0303319W WO 2004013171 A3 WO2004013171 A3 WO 2004013171A3
Authority
WO
WIPO (PCT)
Prior art keywords
subunit protein
insect
interest
expressing
production system
Prior art date
Application number
PCT/GB2003/003319
Other languages
French (fr)
Other versions
WO2004013171A2 (en
Inventor
Roger Craig
Charalambos Savakis
Apostolos Klinakis
Original Assignee
Minos Biosystems Ltd
Roger Craig
Charalambos Savakis
Apostolos Klinakis
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Minos Biosystems Ltd, Roger Craig, Charalambos Savakis, Apostolos Klinakis filed Critical Minos Biosystems Ltd
Priority to AU2003248979A priority Critical patent/AU2003248979A1/en
Publication of WO2004013171A2 publication Critical patent/WO2004013171A2/en
Publication of WO2004013171A3 publication Critical patent/WO2004013171A3/en

Links

Classifications

    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
    • A01K67/60—New or modified breeds of invertebrates
    • A01K67/61—Genetically modified invertebrates, e.g. transgenic or polyploid
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/42—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against immunoglobulins
    • C07K16/4283—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against immunoglobulins against an allotypic or isotypic determinant on Ig
    • C07K16/4291—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against immunoglobulins against an allotypic or isotypic determinant on Ig against IgE
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00—Genetically modified animals
    • A01K2217/05—Animals comprising random inserted nucleic acids (transgenic)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Genetics & Genomics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Environmental Sciences (AREA)
  • Zoology (AREA)
  • Animal Husbandry (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)

Abstract

The invention relates to a method for producing a multi-subunit protein of interest, comprising a method for producing a multi-subunit protein, comprising the steps of (a) transforming a first insect with a non-viral expression system capable of expressing at least one sub-unit, a variant or a fragment thereof, of a multi-subunit protein of interest in the larva of the insect; (b) transforming a second insect with a non-viral expression system capable of expressing at least one sub-unit, of a multi-subunit protein of interest in the larva of the insect; and (c) optionally, transforming a third or further insect with a non-viral expression system capable of expressing at least one sub-unit, of a multi­subunit protein of interest in the larva of the insect; and (d) crossing the insects described in steps a, b and c to produce larvae capable of expressing two or more of said at least one subunits, (e) culturing the larvae; and (f) isolating the multi-subunit protein of interest from the cultured larvae.
PCT/GB2003/003319 2002-08-02 2003-08-01 Multi-subunit protein production system in transgenic insect WO2004013171A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2003248979A AU2003248979A1 (en) 2002-08-02 2003-08-01 Multi-subunit protein production system in transgenic insect

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GBGB0218030.5A GB0218030D0 (en) 2002-08-02 2002-08-02 Multi-submit protein production system
GB0218030.5 2002-08-02

Publications (2)

Publication Number Publication Date
WO2004013171A2 WO2004013171A2 (en) 2004-02-12
WO2004013171A3 true WO2004013171A3 (en) 2004-06-10

Family

ID=9941679

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/GB2003/003319 WO2004013171A2 (en) 2002-08-02 2003-08-01 Multi-subunit protein production system in transgenic insect

Country Status (3)

Country Link
AU (1) AU2003248979A1 (en)
GB (1) GB0218030D0 (en)
WO (1) WO2004013171A2 (en)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SI2311874T1 (en) 2004-07-22 2017-12-29 Erasmus University Medical Center Rotterdam Department of Cell Biology and Genetics Binding molecules
CN104381221B (en) * 2014-10-11 2017-01-25 中国农业科学院植物保护研究所 Bactrocera dorsalis large-scale feeding method
AU2021237476A1 (en) * 2020-03-15 2022-09-29 Proteinea, Inc. Recombinant protein production in insects

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2724182A1 (en) * 1994-09-02 1996-03-08 Pasteur Institut OBTAINING RECOMBINANT MONOCLONAL ANTIBODY FROM ANTI-RHESUS HUMAN MONOCLONAL ANTIBODY, ITS INSECT CELL PRODUCTION, AND ITS USES
WO2001029204A2 (en) * 1999-10-19 2001-04-26 Minos Biosystems Limited Protein production system
US20020001824A1 (en) * 2000-03-18 2002-01-03 Eva-Maria Franken Ligand-gated anion channels of insects
WO2002040679A2 (en) * 2000-11-15 2002-05-23 Archer-Daniels-Midland Company Corynebacterium glutamicum promoters
WO2002040528A1 (en) * 2000-10-26 2002-05-23 Shanghai Institute Of Biochemistry, Chinese Academy Of Sciences A method of producing non-natural silk by bombyx mori
EP1210875A1 (en) * 2000-12-04 2002-06-05 Aventis CropScience GmbH Modulating gene expression in insects by using double-stranded RNA (dsRNA)

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2724182A1 (en) * 1994-09-02 1996-03-08 Pasteur Institut OBTAINING RECOMBINANT MONOCLONAL ANTIBODY FROM ANTI-RHESUS HUMAN MONOCLONAL ANTIBODY, ITS INSECT CELL PRODUCTION, AND ITS USES
WO2001029204A2 (en) * 1999-10-19 2001-04-26 Minos Biosystems Limited Protein production system
US20020001824A1 (en) * 2000-03-18 2002-01-03 Eva-Maria Franken Ligand-gated anion channels of insects
WO2002040528A1 (en) * 2000-10-26 2002-05-23 Shanghai Institute Of Biochemistry, Chinese Academy Of Sciences A method of producing non-natural silk by bombyx mori
WO2002040679A2 (en) * 2000-11-15 2002-05-23 Archer-Daniels-Midland Company Corynebacterium glutamicum promoters
EP1210875A1 (en) * 2000-12-04 2002-06-05 Aventis CropScience GmbH Modulating gene expression in insects by using double-stranded RNA (dsRNA)

Non-Patent Citations (6)

* Cited by examiner, † Cited by third party
Title
CHRISTOPHIDES G K ET AL: "Expression and function of the Drosophila melanogaster ADH in male Ceratitis capitata adults: a potential strategy for medfly genetic sexing based on gene-transfer technology", INSECT MOLECULAR BIOLOGY, BLACKWELL SCIENTIFIC, OXFORD,, GB, vol. 10, no. 3, June 2001 (2001-06-01), pages 249 - 254, XP002211078, ISSN: 0962-1075 *
DATABASE BIOSIS [online] BIOSCIENCES INFORMATION SERVICE, PHILADELPHIA, PA, US; 2001, ZHAO YUN ET AL: "Altering fibroin heavy chain gene of silkworm Bombyx mori by homologous recombination", XP002261258, Database accession no. PREV200100277793 *
D'AVINO P P ET AL: "ECTOPIC EXPRESSION SYSTEMS IN DROSOPHILA", METHODS IN ENZYMOLOGY, ACADEMIC PRESS INC, SAN DIEGO, CA, US, vol. 306, 1999, pages 129 - 142, XP001036598, ISSN: 0076-6879 *
LI T-R ET AL: "A CONDITIONAL RESCUE SYSTEM REVEALS ESSENTIAL FUNCTIONS FOR THE ECDYSONE RECEPTOR (ECR) GENE DURING MOLTING AND METAMORPHOSIS IN DROSOPHILA", DEVELOPMENT, COMPANY OF BIOLOGISTS, CAMBRIDGE,, GB, vol. 127, no. 13, 2000, pages 2897 - 2905, XP001041206, ISSN: 0950-1991 *
SHENGWU HUAXUE YU SHENGWU WULI XUEBAO, vol. 33, no. 1, 2001, pages 112 - 116, ISSN: 0582-9879 *
STEBBINS M J ET AL: "TETRACYCLINE-INDUCIBLE SYSTEMS FOR DROSOPHILA", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF USA, NATIONAL ACADEMY OF SCIENCE. WASHINGTON, US, vol. 98, no. 19, 11 September 2001 (2001-09-11), pages 10775 - 10780, XP001039651, ISSN: 0027-8424 *

Also Published As

Publication number Publication date
AU2003248979A8 (en) 2004-02-23
AU2003248979A1 (en) 2004-02-23
GB0218030D0 (en) 2002-09-11
WO2004013171A2 (en) 2004-02-12

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