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WO2004093645A3 - Mutants de transposase tn5 et leur utilisation - Google Patents

Mutants de transposase tn5 et leur utilisation Download PDF

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Publication number
WO2004093645A3
WO2004093645A3 PCT/US2004/011879 US2004011879W WO2004093645A3 WO 2004093645 A3 WO2004093645 A3 WO 2004093645A3 US 2004011879 W US2004011879 W US 2004011879W WO 2004093645 A3 WO2004093645 A3 WO 2004093645A3
Authority
WO
WIPO (PCT)
Prior art keywords
tnp
wild
mutants
type
transposase
Prior art date
Application number
PCT/US2004/011879
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English (en)
Other versions
WO2004093645A2 (fr
Inventor
William S Reznikoff
Mindy M Steinger-White
Jeremy D Metzler
Original Assignee
Wisconsin Alumni Res Found
William S Reznikoff
Mindy M Steinger-White
Jeremy D Metzler
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Wisconsin Alumni Res Found, William S Reznikoff, Mindy M Steinger-White, Jeremy D Metzler filed Critical Wisconsin Alumni Res Found
Publication of WO2004093645A2 publication Critical patent/WO2004093645A2/fr
Publication of WO2004093645A3 publication Critical patent/WO2004093645A3/fr

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases RNAses, DNAses

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Peptides Or Proteins (AREA)

Abstract

L'invention concerne des mutants de transposase (Tnp) Tn5 présentant une activité de transposase plus élevée que celle de la Tnp du type sauvage. Les mutants de TnpTn5 diffèrent de la Tnp du type sauvage aux positions d'acide aminé 54, 242 et 372 et présentent, par rapport à la Tnp du type sauvage, une avidité supérieure vis-à-vis d'une séquence d'extrémité extérieure de Tn5 du type sauvage telle que définie par SEQ ID NO:3 et/ou une séquence d'extrémité extérieure de Tn5 modifiée telle que définie par SEQ ID NO:5. L'invention concerne également différents systèmes et procédés pour l'utilisation des mutants de Tnp à des fins de transposition in vitro ou in vivo.
PCT/US2004/011879 2003-04-17 2004-04-16 Mutants de transposase tn5 et leur utilisation WO2004093645A2 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US46347003P 2003-04-17 2003-04-17
US60/463,470 2003-04-17

Publications (2)

Publication Number Publication Date
WO2004093645A2 WO2004093645A2 (fr) 2004-11-04
WO2004093645A3 true WO2004093645A3 (fr) 2005-03-31

Family

ID=33310782

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2004/011879 WO2004093645A2 (fr) 2003-04-17 2004-04-16 Mutants de transposase tn5 et leur utilisation

Country Status (2)

Country Link
US (1) US7083980B2 (fr)
WO (1) WO2004093645A2 (fr)

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Citations (3)

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US5965443A (en) * 1996-09-09 1999-10-12 Wisconsin Alumni Research Foundation System for in vitro transposition
US6159736A (en) * 1998-09-23 2000-12-12 Wisconsin Alumni Research Foundation Method for making insertional mutations using a Tn5 synaptic complex
US6406896B1 (en) * 1999-08-02 2002-06-18 Wisconsin Alumni Research Foundation Transposase enzyme and method for use

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Patent Citations (3)

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US5965443A (en) * 1996-09-09 1999-10-12 Wisconsin Alumni Research Foundation System for in vitro transposition
US6159736A (en) * 1998-09-23 2000-12-12 Wisconsin Alumni Research Foundation Method for making insertional mutations using a Tn5 synaptic complex
US6406896B1 (en) * 1999-08-02 2002-06-18 Wisconsin Alumni Research Foundation Transposase enzyme and method for use

Also Published As

Publication number Publication date
US20040235103A1 (en) 2004-11-25
US7083980B2 (en) 2006-08-01
WO2004093645A2 (fr) 2004-11-04

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