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WO2003008639A3 - Method for detecting a nucleic acid - Google Patents

Method for detecting a nucleic acid Download PDF

Info

Publication number
WO2003008639A3
WO2003008639A3 PCT/EP2002/007801 EP0207801W WO03008639A3 WO 2003008639 A3 WO2003008639 A3 WO 2003008639A3 EP 0207801 W EP0207801 W EP 0207801W WO 03008639 A3 WO03008639 A3 WO 03008639A3
Authority
WO
WIPO (PCT)
Prior art keywords
nucleic acid
detecting
fluorophore
acceptor
probe
Prior art date
Application number
PCT/EP2002/007801
Other languages
German (de)
French (fr)
Other versions
WO2003008639A2 (en
Inventor
Thomas Schmitt-John
Regina Plessow
Ralf Palmisano
Andreas Brockhinke
Juergen Weidner
Original Assignee
Praenadia Gmbh
Thomas Schmitt-John
Regina Plessow
Ralf Palmisano
Andreas Brockhinke
Juergen Weidner
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Praenadia Gmbh, Thomas Schmitt-John, Regina Plessow, Ralf Palmisano, Andreas Brockhinke, Juergen Weidner filed Critical Praenadia Gmbh
Publication of WO2003008639A2 publication Critical patent/WO2003008639A2/en
Publication of WO2003008639A3 publication Critical patent/WO2003008639A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6881—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for tissue or cell typing, e.g. human leukocyte antigen [HLA] probes

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Analytical Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

A method for detecting a nucleic acid, wherein the nucleic acid is hybridized with at least one first probe with a donor fluorophore and a second probe with an acceptor fluorphore and the emission spectrum of the donor fluorophore overlaps with the excitation spectrum of the acceptor fluorophore. The donor fluorophore is then selectively excited and the emission of the acceptor fluorophore is detected.
PCT/EP2002/007801 2001-07-12 2002-07-12 Method for detecting a nucleic acid WO2003008639A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE10133308A DE10133308A1 (en) 2001-07-12 2001-07-12 Identifying target cells, useful e.g. for prenatal diagnosis, comprises hybridizing a cellular nucleic acid with energy-transfer donor and acceptor probes
DE10133308.0 2001-07-12

Publications (2)

Publication Number Publication Date
WO2003008639A2 WO2003008639A2 (en) 2003-01-30
WO2003008639A3 true WO2003008639A3 (en) 2003-09-18

Family

ID=7691175

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2002/007801 WO2003008639A2 (en) 2001-07-12 2002-07-12 Method for detecting a nucleic acid

Country Status (2)

Country Link
DE (1) DE10133308A1 (en)
WO (1) WO2003008639A2 (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2008284024A1 (en) * 2007-08-03 2009-02-12 Novartis Ag In-situ hybridization to detect RNA and DNA markers

Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1995003431A1 (en) * 1993-07-19 1995-02-02 Aprogenex, Inc. Enriching and identifying fetal cells in maternal blood for in situ hybridization
EP0745690A2 (en) * 1995-05-12 1996-12-04 The Public Health Research Institute Of The City Of New York, Inc. Detectably labeled dual conformation oligonucleotide Probes, Assays and Kits
US5728527A (en) * 1993-07-20 1998-03-17 University Of Massachusetts Medical Center Detection of hybridized oligonocleotide probes in living cells
US5804386A (en) * 1997-01-15 1998-09-08 Incyte Pharmaceuticals, Inc. Sets of labeled energy transfer fluorescent primers and their use in multi component analysis
US5858649A (en) * 1992-07-17 1999-01-12 Aprogenex, Inc. Amplification of mRNA for distinguishing fetal cells in maternal blood
EP1067134A2 (en) * 1991-11-07 2001-01-10 Nanotronics, Inc. Hybridization of polynucleotides conjugated with chromophores and fluorophores to generate donor-to-donor energy transfer system
US6228592B1 (en) * 1999-05-12 2001-05-08 Laboratory Of Molecular Biophotonics Nucleic acid detection in cytoplasm
EP1172445A1 (en) * 2000-07-14 2002-01-16 Praenadia GmbH A method for direct genetic analysis of target cells by using fluorescence probes

Patent Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1067134A2 (en) * 1991-11-07 2001-01-10 Nanotronics, Inc. Hybridization of polynucleotides conjugated with chromophores and fluorophores to generate donor-to-donor energy transfer system
US5858649A (en) * 1992-07-17 1999-01-12 Aprogenex, Inc. Amplification of mRNA for distinguishing fetal cells in maternal blood
WO1995003431A1 (en) * 1993-07-19 1995-02-02 Aprogenex, Inc. Enriching and identifying fetal cells in maternal blood for in situ hybridization
US5728527A (en) * 1993-07-20 1998-03-17 University Of Massachusetts Medical Center Detection of hybridized oligonocleotide probes in living cells
EP0745690A2 (en) * 1995-05-12 1996-12-04 The Public Health Research Institute Of The City Of New York, Inc. Detectably labeled dual conformation oligonucleotide Probes, Assays and Kits
US5804386A (en) * 1997-01-15 1998-09-08 Incyte Pharmaceuticals, Inc. Sets of labeled energy transfer fluorescent primers and their use in multi component analysis
US6228592B1 (en) * 1999-05-12 2001-05-08 Laboratory Of Molecular Biophotonics Nucleic acid detection in cytoplasm
EP1172445A1 (en) * 2000-07-14 2002-01-16 Praenadia GmbH A method for direct genetic analysis of target cells by using fluorescence probes

Also Published As

Publication number Publication date
WO2003008639A2 (en) 2003-01-30
DE10133308A1 (en) 2003-01-23

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