WO2003050265A3 - Compositions and methods for normalizing assays - Google Patents
Compositions and methods for normalizing assays Download PDFInfo
- Publication number
- WO2003050265A3 WO2003050265A3 PCT/US2002/039671 US0239671W WO03050265A3 WO 2003050265 A3 WO2003050265 A3 WO 2003050265A3 US 0239671 W US0239671 W US 0239671W WO 03050265 A3 WO03050265 A3 WO 03050265A3
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- methods
- assays
- normalizing
- compositions
- enzyme activity
- Prior art date
Links
- 238000000034 method Methods 0.000 title abstract 4
- 238000003556 assay Methods 0.000 title abstract 2
- 239000000203 mixture Substances 0.000 title abstract 2
- 108090000790 Enzymes Proteins 0.000 abstract 2
- 102000004190 Enzymes Human genes 0.000 abstract 2
- 230000000694 effects Effects 0.000 abstract 2
- 230000014509 gene expression Effects 0.000 abstract 2
- 230000002255 enzymatic effect Effects 0.000 abstract 1
- 230000017730 intein-mediated protein splicing Effects 0.000 abstract 1
- 238000010606 normalization Methods 0.000 abstract 1
- 108020004707 nucleic acids Proteins 0.000 abstract 1
- 150000007523 nucleic acids Chemical class 0.000 abstract 1
- 102000039446 nucleic acids Human genes 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/62—DNA sequences coding for fusion proteins
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K2217/00—Genetically modified animals
- A01K2217/05—Animals comprising random inserted nucleic acids (transgenic)
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/20—Fusion polypeptide containing a tag with affinity for a non-protein ligand
- C07K2319/21—Fusion polypeptide containing a tag with affinity for a non-protein ligand containing a His-tag
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/60—Fusion polypeptide containing spectroscopic/fluorescent detection, e.g. green fluorescent protein [GFP]
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
- C07K2319/74—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/90—Fusion polypeptide containing a motif for post-translational modification
- C07K2319/92—Fusion polypeptide containing a motif for post-translational modification containing an intein ("protein splicing")domain
Landscapes
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Plant Pathology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Medicinal Chemistry (AREA)
- Enzymes And Modification Thereof (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Peptides Or Proteins (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
Priority Applications (4)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP2003551287A JP2005512526A (en) | 2001-12-10 | 2002-12-10 | Compositions and methods for standardizing quantitation |
AU2002360565A AU2002360565A1 (en) | 2001-12-10 | 2002-12-10 | Compositions and methods for normalizing assays |
CA002472054A CA2472054A1 (en) | 2001-12-10 | 2002-12-10 | Compositions and methods for normalizing assays |
EP02795831A EP1453952A2 (en) | 2001-12-10 | 2002-12-10 | Compositions and methods for normalizing assays |
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US33983701P | 2001-12-10 | 2001-12-10 | |
US60/339,837 | 2001-12-10 |
Publications (2)
Publication Number | Publication Date |
---|---|
WO2003050265A2 WO2003050265A2 (en) | 2003-06-19 |
WO2003050265A3 true WO2003050265A3 (en) | 2004-02-19 |
Family
ID=23330827
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/US2002/039671 WO2003050265A2 (en) | 2001-12-10 | 2002-12-10 | Compositions and methods for normalizing assays |
Country Status (5)
Country | Link |
---|---|
EP (1) | EP1453952A2 (en) |
JP (1) | JP2005512526A (en) |
AU (1) | AU2002360565A1 (en) |
CA (1) | CA2472054A1 (en) |
WO (1) | WO2003050265A2 (en) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN102712681A (en) * | 2009-11-06 | 2012-10-03 | 谷万达公司 | Intein-modified enzymes, their production and industrial applications |
Families Citing this family (9)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
ES2626067T3 (en) | 2002-01-08 | 2017-07-21 | Agrivida, Inc. | Transgenic plants that express proteins modified with SPIC or intein and related method |
CA2531494A1 (en) * | 2003-07-11 | 2005-01-20 | Novozymes A/S | Method of screening for improved specific activity of enzymes |
US10407742B2 (en) | 2009-11-06 | 2019-09-10 | Agrivida, Inc. | Intein-modified enzymes, their production and industrial applications |
US20130071884A1 (en) | 2009-11-06 | 2013-03-21 | Agrivida, Inc. | Plants expressing cell wall degrading enzymes and expression vectors |
US9464333B2 (en) | 2009-11-06 | 2016-10-11 | Agrivida, Inc. | Intein-modified enzymes, their production and industrial applications |
US9598700B2 (en) | 2010-06-25 | 2017-03-21 | Agrivida, Inc. | Methods and compositions for processing biomass with elevated levels of starch |
US10443068B2 (en) | 2010-06-25 | 2019-10-15 | Agrivida, Inc. | Plants with engineered endogenous genes |
AU2012225487B2 (en) | 2011-03-07 | 2017-04-06 | Agrivida, Inc. | Consolidated pretreatment and hydrolysis of plant biomass expressing cell wall degrading enzymes |
CN111999272A (en) * | 2020-08-13 | 2020-11-27 | 桂林医学院 | Kanamycin detection method |
Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20020177691A1 (en) * | 2001-03-20 | 2002-11-28 | Scott Charles P. | Trans inteins for protein domain shuffling and biopolymerization |
US6562576B2 (en) * | 2001-01-04 | 2003-05-13 | Myriad Genetics, Inc. | Yeast two-hybrid system and use thereof |
-
2002
- 2002-12-10 JP JP2003551287A patent/JP2005512526A/en active Pending
- 2002-12-10 AU AU2002360565A patent/AU2002360565A1/en not_active Abandoned
- 2002-12-10 WO PCT/US2002/039671 patent/WO2003050265A2/en not_active Application Discontinuation
- 2002-12-10 CA CA002472054A patent/CA2472054A1/en not_active Abandoned
- 2002-12-10 EP EP02795831A patent/EP1453952A2/en not_active Withdrawn
Patent Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6562576B2 (en) * | 2001-01-04 | 2003-05-13 | Myriad Genetics, Inc. | Yeast two-hybrid system and use thereof |
US20020177691A1 (en) * | 2001-03-20 | 2002-11-28 | Scott Charles P. | Trans inteins for protein domain shuffling and biopolymerization |
Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN102712681A (en) * | 2009-11-06 | 2012-10-03 | 谷万达公司 | Intein-modified enzymes, their production and industrial applications |
CN102712681B (en) * | 2009-11-06 | 2016-07-06 | 谷万达公司 | Intein modification enzyme and preparation thereof and industrial use |
CN102712682B (en) * | 2009-11-06 | 2016-07-06 | 谷万达公司 | Intein modification enzyme and preparation thereof and industrial use |
Also Published As
Publication number | Publication date |
---|---|
AU2002360565A8 (en) | 2003-06-23 |
AU2002360565A1 (en) | 2003-06-23 |
WO2003050265A2 (en) | 2003-06-19 |
CA2472054A1 (en) | 2003-06-19 |
EP1453952A2 (en) | 2004-09-08 |
JP2005512526A (en) | 2005-05-12 |
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