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WO2002024900A1 - Method of detecting contaminating rice seeds - Google Patents

Method of detecting contaminating rice seeds Download PDF

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Publication number
WO2002024900A1
WO2002024900A1 PCT/JP2001/008038 JP0108038W WO0224900A1 WO 2002024900 A1 WO2002024900 A1 WO 2002024900A1 JP 0108038 W JP0108038 W JP 0108038W WO 0224900 A1 WO0224900 A1 WO 0224900A1
Authority
WO
WIPO (PCT)
Prior art keywords
type genome
japonica
genome domain
rice seeds
domain
Prior art date
Application number
PCT/JP2001/008038
Other languages
French (fr)
Japanese (ja)
Inventor
Naoto Nitta
Toshio Yamamoto
Original Assignee
Japan Tobacco Inc.
Syngenta Limited
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Japan Tobacco Inc., Syngenta Limited filed Critical Japan Tobacco Inc.
Priority to AU2001288050A priority Critical patent/AU2001288050A1/en
Publication of WO2002024900A1 publication Critical patent/WO2002024900A1/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/6895—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for plants, fungi or algae
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/13—Plant traits
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/156—Polymorphic or mutational markers

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Immunology (AREA)
  • Mycology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Botany (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

A method of detecting japonica cultivar rice seeds having no indica type genome domain which contaminate japonica cultivar rice seeds having the indica type genome domain is provided. This method comprises: i) constructing a pair of primers for amplifying the above-described japonica type genome domain or a part thereof based on the base sequence in the japonica type genome domain corresponding to the above-described indica type genome domain; ii) effecting a nucleic acid amplification reaction by using one or more japonica cultivar rice seed genomic DNAs as template(s); and iii) then judging that japonica cultivar rice seeds having no indica type genome domain contaminate, in case where nucleic acid amplification product(s) are detected. Moreover, an improved cetyltrimethylammonium bromide (CTAB) method is provided. This method comprises suspending ground brown rice grains in a buffer for CTAB extraction and then centrifuging at a low temperature to thereby remove starch.
PCT/JP2001/008038 2000-09-22 2001-09-17 Method of detecting contaminating rice seeds WO2002024900A1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2001288050A AU2001288050A1 (en) 2000-09-22 2001-09-17 Method of detecting contaminating rice seeds

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2000/288610 2000-09-22
JP2000288610 2000-09-22

Publications (1)

Publication Number Publication Date
WO2002024900A1 true WO2002024900A1 (en) 2002-03-28

Family

ID=18772172

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/JP2001/008038 WO2002024900A1 (en) 2000-09-22 2001-09-17 Method of detecting contaminating rice seeds

Country Status (2)

Country Link
AU (1) AU2001288050A1 (en)
WO (1) WO2002024900A1 (en)

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS6394942A (en) * 1985-10-30 1988-04-26 フアウエ−　コンビナ−ト　シユテルケ　ウント　カルトフエルフエレ−ドルンク Method for simultaneously obtaining wheat flour and wheat gluten
JPH10286093A (en) * 1997-04-15 1998-10-27 Mitsui Chem Inc Judgment and purity examination of hybrid seed

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS6394942A (en) * 1985-10-30 1988-04-26 フアウエ−　コンビナ−ト　シユテルケ　ウント　カルトフエルフエレ−ドルンク Method for simultaneously obtaining wheat flour and wheat gluten
JPH10286093A (en) * 1997-04-15 1998-10-27 Mitsui Chem Inc Judgment and purity examination of hybrid seed

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
KAZUHIRO KIKUCHI ET AL.: "A rapid and easy-handling procedure for isolation of DNA from rice, arabidopsis and tobacco", PLANT BIOTECHNOLOGY, vol. 15, no. 1, 1998, pages 45 - 48, XP002907450 *
M.G. MURRAY ET AL.: "Rapid isolation of high molecular weight plant DNA", NUCLEIC ACIDS RESEARCH, vol. 8, no. 19, 1998, pages 4321 - 4325, XP002907449 *
MASAKAZU KASHIWABARA: "Shimaha karebyou teikou-sei no ryoushitsu, ryoushoku aji suito hinshu, (Iwata 3 gou) oyobi (Iawata 5 gou) no ikusei", IKUSHUGAKU ZASSHI, vol. 47, SEPARATE VOL. 1, 1997, pages 174, XP002907451 *
N. KURATA ET AL.: "A 300 kilobase interval genetic map of rice including 883 expressed sequences", NATURE GENETICS, vol. 8, no. 4, 1994, pages 365 - 372, XP002907448 *
Y. HAYANO SAITO ET AL.: "Localization of the rice stripe disease resistance gene, Stv-b', by graphical genotyping and linkage analyses with molecular markers", THCOR. APPL. GENET., vol. 96, no. 8, 1998, pages 1044 - 1049, XP002907447 *

Also Published As

Publication number Publication date
AU2001288050A1 (en) 2002-04-02

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