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WO2002096198A3 - Methods for the identification of inhibitors of 3-oxo-5-alpha-steroid 4-dehydrogenase expression or activity in plants - Google Patents

Methods for the identification of inhibitors of 3-oxo-5-alpha-steroid 4-dehydrogenase expression or activity in plants Download PDF

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Publication number
WO2002096198A3
WO2002096198A3 PCT/US2002/016786 US0216786W WO02096198A3 WO 2002096198 A3 WO2002096198 A3 WO 2002096198A3 US 0216786 W US0216786 W US 0216786W WO 02096198 A3 WO02096198 A3 WO 02096198A3
Authority
WO
WIPO (PCT)
Prior art keywords
det2
identification
activity
methods
steroid
Prior art date
Application number
PCT/US2002/016786
Other languages
French (fr)
Other versions
WO2002096198A2 (en
Inventor
Robert Ascenzi
Douglas Boyes
Neil Hoffman
Keith Davis
Adel Zayed
Rao Mulpuri
Jeffrey Woessner
Jorn Gorlach
Carol Hamilton
Kenneth Phillips
Original Assignee
Paradigm Genetics Inc
Robert Ascenzi
Douglas Boyes
Neil Hoffman
Keith Davis
Adel Zayed
Rao Mulpuri
Jeffrey Woessner
Jorn Gorlach
Carol Hamilton
Kenneth Phillips
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Paradigm Genetics Inc, Robert Ascenzi, Douglas Boyes, Neil Hoffman, Keith Davis, Adel Zayed, Rao Mulpuri, Jeffrey Woessner, Jorn Gorlach, Carol Hamilton, Kenneth Phillips filed Critical Paradigm Genetics Inc
Priority to AU2002312111A priority Critical patent/AU2002312111A1/en
Priority to EP02739465A priority patent/EP1407270A4/en
Publication of WO2002096198A2 publication Critical patent/WO2002096198A2/en
Priority to US10/717,844 priority patent/US20040265789A1/en
Publication of WO2002096198A3 publication Critical patent/WO2002096198A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/26—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase
    • C12Q1/32—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase involving dehydrogenase
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/573—Immunoassay; Biospecific binding assay; Materials therefor for enzymes or isoenzymes
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2500/00—Screening for compounds of potential therapeutic value
    • G01N2500/04—Screening involving studying the effect of compounds C directly on molecule A (e.g. C are potential ligands for a receptor A, or potential substrates for an enzyme A)
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2800/00—Detection or diagnosis of diseases
    • G01N2800/52—Predicting or monitoring the response to treatment, e.g. for selection of therapy based on assay results in personalised medicine; Prognosis

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Zoology (AREA)
  • General Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Microbiology (AREA)
  • Urology & Nephrology (AREA)
  • Analytical Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Hematology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • General Engineering & Computer Science (AREA)
  • Cell Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Steroid Compounds (AREA)

Abstract

The present inventors have discovered that 3-oxo-5-alpha-steroid 4-dehydrogenase (DET2) is essential for plant growth. Specifically, the inhibition of DET2 gene expression in plant seedlings resulted in seedlings that are shorter than controls and chlorotic. Thus, DET2 can be used as a target for the identification of herbicides. Accordingly, the present invention provides methods for the identification of compounds that inhibit DET2 expression or activity, comprising: contacting a compound with a DET2 and detecting the presence and/or absence of binding between said compound and said DET2, or detecting a decrease in DET2 expression or activity. The methods of the invention are useful for the identification of herbicides.
PCT/US2002/016786 2001-05-30 2002-05-30 Methods for the identification of inhibitors of 3-oxo-5-alpha-steroid 4-dehydrogenase expression or activity in plants WO2002096198A2 (en)

Priority Applications (3)

Application Number Priority Date Filing Date Title
AU2002312111A AU2002312111A1 (en) 2001-05-30 2002-05-30 Methods for the identification of inhibitors of 3-oxo-5-alpha-steroid 4-dehydrogenase expression or activity in plants
EP02739465A EP1407270A4 (en) 2001-05-30 2002-05-30 Methods for the identification of inhibitors of 3-oxo-5-alpha-steroid 4-dehydrogenase expression or activity in plants
US10/717,844 US20040265789A1 (en) 2002-05-30 2003-11-20 Methods for the identification of inhibitors of 3-oxo-5-alpha-steroid 4-dehydrogenase expression or activity in plants

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US29439501P 2001-05-30 2001-05-30
US60/294,395 2001-05-30

Publications (2)

Publication Number Publication Date
WO2002096198A2 WO2002096198A2 (en) 2002-12-05
WO2002096198A3 true WO2002096198A3 (en) 2004-02-12

Family

ID=23133227

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2002/016786 WO2002096198A2 (en) 2001-05-30 2002-05-30 Methods for the identification of inhibitors of 3-oxo-5-alpha-steroid 4-dehydrogenase expression or activity in plants

Country Status (3)

Country Link
EP (1) EP1407270A4 (en)
AU (1) AU2002312111A1 (en)
WO (1) WO2002096198A2 (en)

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5730964A (en) * 1994-07-21 1998-03-24 Merck & Co., Inc. Method of treating sweat-related conditions
US6187811B1 (en) * 1998-10-28 2001-02-13 Lipogenics, Inc. Methods for treating benign prostatic hyperplasia using tocotrienols

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5952545A (en) * 1996-03-27 1999-09-14 Max-Planck-Gesellschaft Zur Forderung Der Wissenschaften E.V. Nucleic acid molecules encoding cytochrome P450-type proteins involved in the brassinosteroid synthesis in plants
US6143950A (en) * 1996-04-18 2000-11-07 The Salk Institute For Biological Studies Plant steroid 5α reductase, DET2
ITFI970193A1 (en) * 1997-08-01 1999-02-01 Applied Research Systems USE OF BENZO (C) QUINOLYZINE DERIVATIVES AS REGULATORS OF PLANT GROWTH AND COMPOSITIONS FOR AGRICULTURAL USE CONTAINING SUCH
JP2002518054A (en) * 1998-06-24 2002-06-25 シンジェンタ パーティシペーションズ アクチェンゲゼルシャフト Method for screening herbicidal compounds using AIR synthetase from Arabidopsis thaliana
WO2000053782A2 (en) * 1999-03-05 2000-09-14 Syngenta Participations Ag Herbicide target genes and methods
EP1204756A2 (en) * 1999-07-30 2002-05-15 Bayer Aktiengesellschaft Method of determining the activity of 1-deoxy-d-xylulose-5-phosphate reductoisomerase and 1-deoxy-d-xylulose-5-phosphate synthase

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5730964A (en) * 1994-07-21 1998-03-24 Merck & Co., Inc. Method of treating sweat-related conditions
US6187811B1 (en) * 1998-10-28 2001-02-13 Lipogenics, Inc. Methods for treating benign prostatic hyperplasia using tocotrienols

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
LEVY M.A. ET AL.: "Interaction between rat prostatic steroid 5alpha-reductase and 3-carboxy-17beta-substituted steroids: novel mechanism of enzyme inhibition", J. STEROID BIOCHEM., vol. 34, no. 1-6, September 1989 (1989-09-01), pages 571 - 575, XP002961849 *
LIN X. ET AL.: "Sequence and analysis of chromosome 2 of the plant arabidopsis thaliana", NATURE, vol. 402, 16 December 1999 (1999-12-16), pages 761 - 768, XP002952930 *
See also references of EP1407270A4 *
SINGH S.: "Light and cytokinin regulation of leaf senescence and hypocotyl growth in det-2 mutant and wild-type arabidopsis thaliana plants: a laboratory exercise", PLANT PHYSIOLOGY, vol. 114, no. 3, SUPPL., August 1997 (1997-08-01), pages 316, ABSTRACT NO. 1662, XP002961883 *

Also Published As

Publication number Publication date
WO2002096198A2 (en) 2002-12-05
EP1407270A4 (en) 2005-05-25
EP1407270A2 (en) 2004-04-14
AU2002312111A1 (en) 2002-12-09

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