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WO2002082057A3 - Split focusing cytometer - Google Patents

Split focusing cytometer Download PDF

Info

Publication number
WO2002082057A3
WO2002082057A3 PCT/US2002/010508 US0210508W WO02082057A3 WO 2002082057 A3 WO2002082057 A3 WO 2002082057A3 US 0210508 W US0210508 W US 0210508W WO 02082057 A3 WO02082057 A3 WO 02082057A3
Authority
WO
WIPO (PCT)
Prior art keywords
focusing cytometer
split focusing
split
cytometer
white
Prior art date
Application number
PCT/US2002/010508
Other languages
French (fr)
Other versions
WO2002082057A2 (en
Inventor
Bernard H Weigl
Ronald L Bardell
C Frederick Battrell
Original Assignee
Micronics Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Micronics Inc filed Critical Micronics Inc
Priority to DE60227649T priority Critical patent/DE60227649D1/en
Priority to EP02719428A priority patent/EP1377811B1/en
Priority to JP2002579778A priority patent/JP3949056B2/en
Publication of WO2002082057A2 publication Critical patent/WO2002082057A2/en
Publication of WO2002082057A3 publication Critical patent/WO2002082057A3/en

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    • G01N15/05—Investigating sedimentation of particle suspensions in blood
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    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/40—Concentrating samples
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    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/40—Concentrating samples
    • G01N1/4077—Concentrating samples by other techniques involving separation of suspended solids
    • G01N2001/4094—Concentrating samples by other techniques involving separation of suspended solids using ultrasound
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/02—Investigating particle size or size distribution
    • G01N2015/0288—Sorting the particles
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N15/1404—Handling flow, e.g. hydrodynamic focusing
    • G01N15/1409—Handling samples, e.g. injecting samples
    • G01N2015/1411—Features of sheath fluids
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    • G01N15/10—Investigating individual particles
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    • G01N15/1404—Handling flow, e.g. hydrodynamic focusing
    • G01N2015/1413—Hydrodynamic focussing
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    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N15/1434—Optical arrangements
    • G01N2015/144—Imaging characterised by its optical setup
    • G—PHYSICS
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    • G01N15/10—Investigating individual particles
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    • G01N2015/1486—Counting the particles
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    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N2035/00178—Special arrangements of analysers
    • G01N2035/00237—Handling microquantities of analyte, e.g. microvalves, capillary networks
    • G01N2035/00247—Microvalves
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/25—Chemistry: analytical and immunological testing including sample preparation
    • Y10T436/25375—Liberation or purification of sample or separation of material from a sample [e.g., filtering, centrifuging, etc.]
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/25—Chemistry: analytical and immunological testing including sample preparation
    • Y10T436/2575—Volumetric liquid transfer

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  • Chemical & Material Sciences (AREA)
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  • Molecular Biology (AREA)
  • Urology & Nephrology (AREA)
  • Automatic Analysis And Handling Materials Therefor (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Multiple-Way Valves (AREA)
  • Sampling And Sample Adjustment (AREA)
  • Check Valves (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Separation Using Semi-Permeable Membranes (AREA)
  • External Artificial Organs (AREA)

Abstract

A microcytometer which combines lysing and cytometry into a unified system that achieves blood lysis and white blood cell count in a single device. The device focuses the white cells into a thin ribbon which is then focused into a single stream for analysis.
PCT/US2002/010508 2001-04-03 2002-04-03 Split focusing cytometer WO2002082057A2 (en)

Priority Applications (3)

Application Number Priority Date Filing Date Title
DE60227649T DE60227649D1 (en) 2001-04-03 2002-04-03 ISOLATED FOCUSING CYTOMETER
EP02719428A EP1377811B1 (en) 2001-04-03 2002-04-03 Split focusing cytometer
JP2002579778A JP3949056B2 (en) 2001-04-03 2002-04-03 Split concentration cytometer

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US28111401P 2001-04-03 2001-04-03
US60/281,114 2001-04-03

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WO2002082057A2 WO2002082057A2 (en) 2002-10-17
WO2002082057A3 true WO2002082057A3 (en) 2003-02-13

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PCT/US2002/010508 WO2002082057A2 (en) 2001-04-03 2002-04-03 Split focusing cytometer
PCT/US2002/010509 WO2002081934A2 (en) 2001-04-03 2002-04-03 Pneumatic valve interface for use in microfluidic structures

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PCT/US2002/010509 WO2002081934A2 (en) 2001-04-03 2002-04-03 Pneumatic valve interface for use in microfluidic structures

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US (8) US6674525B2 (en)
EP (2) EP1377821A2 (en)
JP (2) JP3949056B2 (en)
AT (1) ATE401566T1 (en)
DE (1) DE60227649D1 (en)
WO (2) WO2002082057A2 (en)

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