[go: up one dir, main page]
More Web Proxy on the site http://driver.im/

WO2002057997A8 - Method and arrangement for segmenting white blood cells in a digital colour image - Google Patents

Method and arrangement for segmenting white blood cells in a digital colour image

Info

Publication number
WO2002057997A8
WO2002057997A8 PCT/SE2002/000050 SE0200050W WO02057997A8 WO 2002057997 A8 WO2002057997 A8 WO 2002057997A8 SE 0200050 W SE0200050 W SE 0200050W WO 02057997 A8 WO02057997 A8 WO 02057997A8
Authority
WO
WIPO (PCT)
Prior art keywords
blood cells
white blood
arrangement
pixels
images
Prior art date
Application number
PCT/SE2002/000050
Other languages
French (fr)
Other versions
WO2002057997A1 (en
Inventor
Bjoern Nilsson
Original Assignee
Cellavision Ab
Bjoern Nilsson
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from SE0100142A external-priority patent/SE518457C2/en
Application filed by Cellavision Ab, Bjoern Nilsson filed Critical Cellavision Ab
Publication of WO2002057997A1 publication Critical patent/WO2002057997A1/en
Publication of WO2002057997A8 publication Critical patent/WO2002057997A8/en

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N15/1429—Signal processing
    • G01N15/1433—Signal processing using image recognition
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/483—Physical analysis of biological material
    • G01N33/487—Physical analysis of biological material of liquid biological material
    • G01N33/49—Blood
    • G01N33/491—Blood by separating the blood components
    • G—PHYSICS
    • G06—COMPUTING; CALCULATING OR COUNTING
    • G06V—IMAGE OR VIDEO RECOGNITION OR UNDERSTANDING
    • G06V20/00—Scenes; Scene-specific elements
    • G06V20/60—Type of objects
    • G06V20/69—Microscopic objects, e.g. biological cells or cellular parts
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/01—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials specially adapted for biological cells, e.g. blood cells
    • G01N2015/012—Red blood cells
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/01—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials specially adapted for biological cells, e.g. blood cells
    • G01N2015/016—White blood cells
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/01—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials specially adapted for biological cells, e.g. blood cells
    • G01N2015/018—Platelets
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N15/1468—Optical investigation techniques, e.g. flow cytometry with spatial resolution of the texture or inner structure of the particle
    • G01N2015/1472—Optical investigation techniques, e.g. flow cytometry with spatial resolution of the texture or inner structure of the particle with colour
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N2015/1488—Methods for deciding
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N2015/1493—Particle size

Landscapes

  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Physics & Mathematics (AREA)
  • General Physics & Mathematics (AREA)
  • General Health & Medical Sciences (AREA)
  • Pathology (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Hematology (AREA)
  • Biochemistry (AREA)
  • Analytical Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Food Science & Technology (AREA)
  • Urology & Nephrology (AREA)
  • Ecology (AREA)
  • Multimedia (AREA)
  • Theoretical Computer Science (AREA)
  • Signal Processing (AREA)
  • Dispersion Chemistry (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Image Processing (AREA)
  • Image Analysis (AREA)

Abstract

The invention relates to a method for segmenting white blood cells in a series of images with at least one digital color image that has pixels with at least two color components, where red blood cells are also to be found in the series of images. The method comprises identifying a first and a second set of pixels in the series of images that depict red and while blood cells respectively using a first and a second rough measure; identifying first and second functions that give an approximate description of the relationship between the intensities of the two color components for pixels in the first and second set respectively; and determining whether a given pixel depicts white blood cells or not, on the basis of the given pixel's intensities of the two color components and the first and second functions.
PCT/SE2002/000050 2001-01-18 2002-01-17 Method and arrangement for segmenting white blood cells in a digital colour image WO2002057997A1 (en)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
SE0100142A SE518457C2 (en) 2001-01-18 2001-01-18 Segmenting method for objects in a first category in a series of images with at least one digital microscope color image that depicts biological material for analyzing peripheral blood and bone marrow
SE0100142-9 2001-01-18
US29031601P 2001-05-11 2001-05-11
US60/290,316 2001-05-11

Publications (2)

Publication Number Publication Date
WO2002057997A1 WO2002057997A1 (en) 2002-07-25
WO2002057997A8 true WO2002057997A8 (en) 2004-05-27

Family

ID=26655375

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/SE2002/000050 WO2002057997A1 (en) 2001-01-18 2002-01-17 Method and arrangement for segmenting white blood cells in a digital colour image

Country Status (1)

Country Link
WO (1) WO2002057997A1 (en)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SE528697C2 (en) 2005-03-11 2007-01-30 Hemocue Ab Volumetric determination of the number of white blood cells in a blood sample
EP2012254B1 (en) * 2007-07-06 2010-09-08 PerkinElmer Cellular Technologies Germany GmbH Method for quantifying an underlying property of a multitude of samples
JP5466825B2 (en) * 2008-01-23 2014-04-09 シスメックス株式会社 Cell image processing system, cell image display system, and cell image display method
US8274656B2 (en) * 2010-06-30 2012-09-25 Luminex Corporation Apparatus, system, and method for increasing measurement accuracy in a particle imaging device
CN108269259A (en) * 2017-12-15 2018-07-10 上海市第六人民医院 Image partition method based on Sections of Bone Marrow fluorescent marker
EP4279901A1 (en) * 2022-05-17 2023-11-22 MEON Functional Diagnostics GmbH Method and apparatus for classifying white blood cells

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4882284A (en) * 1987-04-13 1989-11-21 Ortho Pharmaceutical Corporation Method for quantitating and differentiating white blood cells
US5764792A (en) * 1996-01-19 1998-06-09 Oncor, Inc. Method and apparatus for processing images

Also Published As

Publication number Publication date
WO2002057997A1 (en) 2002-07-25

Similar Documents

Publication Publication Date Title
CN101080022B (en) Image forming apparatus
WO2001013263A3 (en) System and method for performing region-based image retrieval using color-based segmentation.
AU3328299A (en) Method and arrangement for objective assessment of video quality
EP1359543A3 (en) Method for detecting subject matter regions in images
EP1918871A3 (en) Image enhancement method and system
EP1137257A3 (en) Method of and apparatus for image processing
EP0929190A3 (en) Method and apparatus for correcting luminance and chrominance data in digital color images
EP1720128A3 (en) Identifying intrinsic pixel colors in a region of uncertain pixels
EP1079599A3 (en) Image processing apparatus with image region determining function
SG118191A1 (en) Method and system for contrast enhancement of digital video
EP1202217A3 (en) Increasing the spatial resolution of an image using co-registered image of higher resolution
WO2003003300A3 (en) Automatic digital picture enhancement
EP1863269A3 (en) Fade detector for digital video
EP1227439A3 (en) Method of calculating noise from a digital image utilizing color cross correlation statistics
EP1185082A3 (en) Method and apparatus for correcting white balance
EP1215886A3 (en) Automatic color saturation enhancement
WO2002087258A3 (en) Image processing apparatus for and method of improving an image and an image display apparatus comprising the image processing apparatus
EP1439490A3 (en) Shoot suppression in image Enhancement
EP0930775A3 (en) Image processing method and apparatus
EP0923052A3 (en) Image encoding/decoding technique
WO2002057997A8 (en) Method and arrangement for segmenting white blood cells in a digital colour image
WO2002005212A3 (en) Image enhancement
EP1475746A3 (en) Image manipulation according to pixel type
GB9925902D0 (en) Colour image restoration with anti-alias
EP1223552A3 (en) System and method for detecting text edges in digital document images

Legal Events

Date Code Title Description
AK Designated states

Kind code of ref document: A1

Designated state(s): AE AG AL AM AT AT AU AZ BA BB BG BR BY BZ CA CH CN CO CR CU CZ CZ DE DE DK DK DM DZ EC EE EE ES FI FI GB GD GE GH GM HR HU ID IL IN IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MA MD MG MK MN MW MX MZ NO NZ OM PH PL PT RO RU SD SE SG SI SK SK SL TJ TM TN TR TT TZ UA UG US UZ VN YU ZA ZM ZW

AL Designated countries for regional patents

Kind code of ref document: A1

Designated state(s): GH GM KE LS MW MZ SD SL SZ TZ UG ZM ZW AM AZ BY KG KZ MD RU TJ TM AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE TR BF BJ CF CG CI CM GA GN GQ GW ML MR NE SN TD TG

DFPE Request for preliminary examination filed prior to expiration of 19th month from priority date (pct application filed before 20040101)
121 Ep: the epo has been informed by wipo that ep was designated in this application
REG Reference to national code

Ref country code: DE

Ref legal event code: 8642

122 Ep: pct application non-entry in european phase
CFP Corrected version of a pamphlet front page
CR1 Correction of entry in section i

Free format text: IN PCT GAZETTE 30/2002 DUE TO A TECHNICAL PROBLEMAT THE TIME OF INTERNATIONAL PUBLICATION, SOME INFORMATION WAS MISSING UNDER (81). THE MISSING INFORMATION NOW APPEARS IN THE CORRECTED VERSION

NENP Non-entry into the national phase

Ref country code: JP

WWW Wipo information: withdrawn in national office

Country of ref document: JP