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WO2001032886A3 - Starch branching enzymes - Google Patents

Starch branching enzymes Download PDF

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Publication number
WO2001032886A3
WO2001032886A3 PCT/CA2000/001276 CA0001276W WO0132886A3 WO 2001032886 A3 WO2001032886 A3 WO 2001032886A3 CA 0001276 W CA0001276 W CA 0001276W WO 0132886 A3 WO0132886 A3 WO 0132886A3
Authority
WO
WIPO (PCT)
Prior art keywords
enzyme
starch branching
branching enzymes
wheat
starch granules
Prior art date
Application number
PCT/CA2000/001276
Other languages
French (fr)
Other versions
WO2001032886A2 (en
Inventor
Monica Baga
Mingsheng Peng
Ramesh B Nair
Anne Repellin
Ming Gao
Pierre Hucl
Graham J Scoles
Ravindra N Chibbar
Original Assignee
Ca Nat Research Council
Monica Baga
Mingsheng Peng
Ramesh B Nair
Anne Repellin
Ming Gao
Pierre Hucl
Graham J Scoles
Ravindra N Chibbar
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Ca Nat Research Council, Monica Baga, Mingsheng Peng, Ramesh B Nair, Anne Repellin, Ming Gao, Pierre Hucl, Graham J Scoles, Ravindra N Chibbar filed Critical Ca Nat Research Council
Priority to US10/110,777 priority Critical patent/US7041484B1/en
Priority to AU11227/01A priority patent/AU1122701A/en
Priority to CA002389390A priority patent/CA2389390A1/en
Publication of WO2001032886A2 publication Critical patent/WO2001032886A2/en
Publication of WO2001032886A3 publication Critical patent/WO2001032886A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C12N9/1051—Hexosyltransferases (2.4.1)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8245—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving modified carbohydrate or sugar alcohol metabolism, e.g. starch biosynthesis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C12N9/1051—Hexosyltransferases (2.4.1)
    • C12N9/107—1,4-Alpha-glucan branching enzyme (2.4.1.18)

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Nutrition Science (AREA)
  • Cell Biology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Botany (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

The invention provides a novel starch branching enzyme that is bound to A-type starch granules in wheat, barley, rye or triticale. The enzyme is not substantially associated with B-type starch granules. A cDNA sequence encoding an isoform of the enzyme has been isolated from the wheat cultivar Fielder and deduced amino acid sequence has been determined.
PCT/CA2000/001276 1999-10-29 2000-10-27 Starch branching enzymes WO2001032886A2 (en)

Priority Applications (3)

Application Number Priority Date Filing Date Title
US10/110,777 US7041484B1 (en) 1999-10-29 2000-10-27 Starch branching enzymes
AU11227/01A AU1122701A (en) 1999-10-29 2000-10-27 Starch branching enzymes
CA002389390A CA2389390A1 (en) 1999-10-29 2000-10-27 Starch branching enzymes

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US16214499P 1999-10-29 1999-10-29
US60/162,144 1999-10-29

Publications (2)

Publication Number Publication Date
WO2001032886A2 WO2001032886A2 (en) 2001-05-10
WO2001032886A3 true WO2001032886A3 (en) 2001-11-29

Family

ID=22584342

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/CA2000/001276 WO2001032886A2 (en) 1999-10-29 2000-10-27 Starch branching enzymes

Country Status (3)

Country Link
AU (1) AU1122701A (en)
CA (1) CA2389390A1 (en)
WO (1) WO2001032886A2 (en)

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AUPQ005299A0 (en) 1999-04-29 1999-05-27 Commonwealth Scientific And Industrial Research Organisation Novel genes encoding wheat starch synthases and uses therefor
ES2380362T3 (en) 2000-11-09 2012-05-11 Commonwealth Scientific And Industrial Research Organisation Barley with reduced activity in SSII and products containing starch with reduced amylopectin content
AUPS219802A0 (en) 2002-05-09 2002-06-06 Commonwealth Scientific And Industrial Research Organisation Barley with altered branching enzyme activity and starch and starch containing products with a reduced amylopectin content
US7482116B2 (en) 2002-06-07 2009-01-27 Dna Genotek Inc. Compositions and methods for obtaining nucleic acids from sputum
AU2004252186B8 (en) 2003-06-30 2010-06-24 Commonwealth Scientific And Industrial Research Organisation Wheat with altered branching enzyme activity and starch and starch containing products derived therefrom
JP2007511207A (en) 2003-10-27 2007-05-10 コモンウェルス サイエンティフィック アンド インダストリアル リサーチ オーガナイゼイション Rice with starch with increased amylose ratio and its products
US7700139B2 (en) 2004-12-30 2010-04-20 Commonwealth Scientific And Industrial Research Organization Method and means for improving bowel health
US7993686B2 (en) 2004-12-30 2011-08-09 Commonwealth Scientific And Industrial Organisation Method and means for improving bowel health
CA2653883C (en) 2008-07-17 2022-04-05 Colin Leslie Dow Jenkins High fructan cereal plants
EP2458974A4 (en) 2009-07-30 2013-04-03 Commw Scient Ind Res Org BARLEY AND ITS USES
BR112013011025B1 (en) 2010-11-04 2021-03-02 Arista Cereal Technologies Pty Ltd method for producing wheat flour, wheat flour, food ingredient, food product, food production method and starch production method
WO2012103594A1 (en) 2011-02-03 2012-08-09 Commonwealth Scientific And Industrial Research Organisation Barley with modified ssiii
PL2764106T3 (en) 2011-10-04 2019-09-30 Arcadia Biosciences Inc. Wheat with increased resistant starch levels
WO2013063653A1 (en) 2011-11-04 2013-05-10 Arista Cereal Technologies Pty Limited High amylose wheat - ii

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1994009144A1 (en) * 1992-10-14 1994-04-28 Zeneca Limited Novel plants and processes for obtaining them
WO1997022703A2 (en) * 1995-12-20 1997-06-26 E.I. Du Pont De Nemours And Company Novel starches via modification of expression of starch biosynthetic enzyme genes
WO1998014601A1 (en) * 1996-09-30 1998-04-09 Exseed Genetics L.L.C. Encapsulation of polypeptides within the starch matrix
WO1999014314A1 (en) * 1997-09-12 1999-03-25 Commonwealth Scientific And Industrial Research Organisation Regulation of gene expression in plants

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1994009144A1 (en) * 1992-10-14 1994-04-28 Zeneca Limited Novel plants and processes for obtaining them
WO1997022703A2 (en) * 1995-12-20 1997-06-26 E.I. Du Pont De Nemours And Company Novel starches via modification of expression of starch biosynthetic enzyme genes
WO1998014601A1 (en) * 1996-09-30 1998-04-09 Exseed Genetics L.L.C. Encapsulation of polypeptides within the starch matrix
WO1999014314A1 (en) * 1997-09-12 1999-03-25 Commonwealth Scientific And Industrial Research Organisation Regulation of gene expression in plants

Non-Patent Citations (13)

* Cited by examiner, † Cited by third party
Title
BAGA M ET AL: "WHEAT STARCH MODIFICATION THROUGH BIOTECHNOLOGY", STARCH STARKE,DE,WILEY-VCH VERLAG, WEINHEIM, vol. 51, no. 4, April 1999 (1999-04-01), pages 111 - 116, XP000828602, ISSN: 0038-9056 *
BAGA MONICA ET AL: "A starch-branching enzyme gene in wheat produces alternatively spliced transcripts.", PLANT MOLECULAR BIOLOGY, vol. 40, no. 6, August 1999 (1999-08-01), pages 1019 - 1030, XP002172355, ISSN: 0167-4412 *
BAGA MONICA ET AL: "Isolation of a cDNA encoding a granule-bound 152-kilodalton starch-branching enzyme in wheat.", PLANT PHYSIOLOGY (ROCKVILLE), vol. 124, no. 1, September 2000 (2000-09-01), pages 253 - 263, XP002172352, ISSN: 0032-0889 *
DATABASE EMBL 14 May 1999 (1999-05-14), RAHMAN, S., ET AL.: "Triticum aestivum starch branching enzyme-I (SBE-I) mRNA, complete cds.", XP002172360 *
DATABASE EMBL 3 July 1997 (1997-07-03), RAHMAN S., ET AL.: "Triticum aestivum starch branching enzyme I (wSBE I-D2) mRNA, complete cds.", XP002172359 *
DATABASE EMBL 9 April 1997 (1997-04-09), REPELLIN, A., ET AL.: "T.aestivum mRNA for starch branching enzyme I", XP002172358 *
MU-FORSTER C ET AL: "PHYSICAL ASSOCIATION OF STARCH BIOSYNTHETIC ENZYMES WITH STARCH GRANULES OF MAIZE ENDOSPERM", PLANT PHYSIOLOGY,US,AMERICAN SOCIETY OF PLANT PHYSIOLOGISTS, ROCKVILLE, MD, vol. 111, 1996, pages 821 - 829, XP002056414, ISSN: 0032-0889 *
PENG MINGSHENG ET AL: "Starch-branching enzymes preferentially associated with A-type starch granules in wheat endosperm.", PLANT PHYSIOLOGY (ROCKVILLE), vol. 124, no. 1, September 2000 (2000-09-01), pages 265 - 272, XP002172353, ISSN: 0032-0889 *
RAHMAN SADEQUR ET AL: "The major proteins of wheat endosperm starch granules.", AUSTRALIAN JOURNAL OF PLANT PHYSIOLOGY, vol. 22, no. 5, 1995, pages 793 - 803, XP002043127, ISSN: 0310-7841 *
RAHMAN, S., ET AL.: "Characterisation of a gene encoding wheat endosperm branching enzyme-I", THEOR. APPL. GENET., vol. 98, 1999, pages 156 - 163, XP001010371 *
REPELLIN, A., ET AL.: "Isolation of a Starch Branching Enzyme I cDNA from a Wheat Endosperm library (Accession No. Y12320) (PGR97-094).", PLANT GENE REGISTER PGR97-094 PLANT PHYSIOL. 114: 1135, 1997, XP002172354 *
SCHOFIELD J D ET AL: "WHEAT STARCH GRANULE PROTEINS AND THEIR TECHNOLOGICAL SIGNIFICANCE", 89573-523-7. 1987, 1987, BOURNEMOUTH, ENGLAND, UK, 1986. VIII+523P. ELLIS HORWOOD LTD.: CHICHESTER, ENGLAND, UK (DIST BY VCH VERLAGSGESELLSCHAFT: WEINHEIM, WEST GERMANY; VCH PUBLISHERS: NEW YORK, NEW YORK, USA). ILLUS. ISBN 0, pages 407 - 420, XP001014172 *
TAKAOKA MOTOKO ET AL: "Structural characterization of high molecular weight starch granule-bound proteins in wheat (Triticum aestivum L.).", JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, vol. 45, no. 8, 1997, pages 2929 - 2934, XP002172356, ISSN: 0021-8561 *

Also Published As

Publication number Publication date
AU1122701A (en) 2001-05-14
WO2001032886A2 (en) 2001-05-10
CA2389390A1 (en) 2001-05-10

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