[go: up one dir, main page]
More Web Proxy on the site http://driver.im/

WO2001044852A3 - Microscopy method and device - Google Patents

Microscopy method and device Download PDF

Info

Publication number
WO2001044852A3
WO2001044852A3 PCT/EP2000/012120 EP0012120W WO0144852A3 WO 2001044852 A3 WO2001044852 A3 WO 2001044852A3 EP 0012120 W EP0012120 W EP 0012120W WO 0144852 A3 WO0144852 A3 WO 0144852A3
Authority
WO
WIPO (PCT)
Prior art keywords
image
sample
dimensions
recorded
focal plane
Prior art date
Application number
PCT/EP2000/012120
Other languages
German (de)
French (fr)
Other versions
WO2001044852A2 (en
Inventor
Achim Kirsch
Juergen Mueller
Original Assignee
Evotec Biosystems Ag
Achim Kirsch
Juergen Mueller
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Evotec Biosystems Ag, Achim Kirsch, Juergen Mueller filed Critical Evotec Biosystems Ag
Priority to DE20023019U priority Critical patent/DE20023019U1/en
Publication of WO2001044852A2 publication Critical patent/WO2001044852A2/en
Publication of WO2001044852A3 publication Critical patent/WO2001044852A3/en

Links

Classifications

    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/18—Arrangements with more than one light path, e.g. for comparing two specimens
    • G02B21/20—Binocular arrangements
    • G02B21/22—Stereoscopic arrangements

Landscapes

  • Physics & Mathematics (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Optics & Photonics (AREA)
  • Microscoopes, Condenser (AREA)

Abstract

The invention provides that in order to optically image a sample (140) in three-dimensions, the entire sample (140) is uniformly illuminated, a conventional first partial image is recorded, and the sample (140) is subjected to a laterally structured illumination in a focal plane. The illumination of the individual points of the focal plane is varied according to predetermined temporal sequences. A second partial image is recorded by detecting the light emitted by the object points that are not illuminated in the confocal plane. An image of the probe (140) that is spatially resolved in three-dimensions is established from the first and second partial images. The invention also relates to optical imaging systems used for carrying out this imaging method.
PCT/EP2000/012120 1999-12-15 2000-12-01 Microscopy method and device WO2001044852A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
DE20023019U DE20023019U1 (en) 1999-12-15 2000-12-01 Microscopy device

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE19960583.1 1999-12-15
DE1999160583 DE19960583A1 (en) 1999-12-15 1999-12-15 Method and device for microscopy

Publications (2)

Publication Number Publication Date
WO2001044852A2 WO2001044852A2 (en) 2001-06-21
WO2001044852A3 true WO2001044852A3 (en) 2001-12-06

Family

ID=7932808

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2000/012120 WO2001044852A2 (en) 1999-12-15 2000-12-01 Microscopy method and device

Country Status (2)

Country Link
DE (2) DE19960583A1 (en)
WO (1) WO2001044852A2 (en)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2001067155A1 (en) 2000-03-06 2001-09-13 Olympus Optical Co., Ltd. Pattern forming member applied to sectioning image observing device and sectioning image observing device using it
US6778724B2 (en) 2000-11-28 2004-08-17 The Regents Of The University Of California Optical switching and sorting of biological samples and microparticles transported in a micro-fluidic device, including integrated bio-chip devices
CA2536360C (en) 2003-08-28 2013-08-06 Celula, Inc. Methods and apparatus for sorting cells using an optical switch in a microfluidic channel network
DE102005040471B4 (en) * 2005-08-26 2007-06-21 Leica Microsystems (Schweiz) Ag microscope
US7593156B2 (en) 2005-08-26 2009-09-22 Leica Microsystems (Schweiz) Ag Microscope with micro-mirrors for optional deflection and/or beam splitting
DE102006022592B4 (en) 2006-05-15 2008-02-07 Leica Microsystems (Schweiz) Ag Microscope with lighting unit
DE102006022590C5 (en) * 2006-05-15 2010-05-12 Leica Microsystems (Schweiz) Ag Lighting unit for a microscope

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0911667A1 (en) * 1997-10-22 1999-04-28 Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V. Programmable spatially light modulated microscope and microscopy method

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0485803B1 (en) * 1990-11-10 1996-05-01 Grosskopf, Rudolf, Dr.-Ing. Optical scanning device with confocal beam path using a light source array and a detector array
US5587832A (en) * 1993-10-20 1996-12-24 Biophysica Technologies, Inc. Spatially light modulated confocal microscope and method
DE9406545U1 (en) * 1994-04-20 1994-11-03 Max-Planck-Gesellschaft zur Förderung der Wissenschaften eV, 14195 Berlin Contrast device for microscopy
GB9603788D0 (en) * 1996-02-22 1996-04-24 Isis Innovation Confocal microscope
DE19824460A1 (en) * 1998-05-30 1999-12-02 Zeiss Carl Jena Gmbh Arrangement and method for the microscopic generation of object images

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0911667A1 (en) * 1997-10-22 1999-04-28 Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V. Programmable spatially light modulated microscope and microscopy method

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
JUSKAITIS R ET AL: "EFFICIENT REAL-TIME CONFOCAL MICROSCOPY WITH WHITE LIGHT SOURCES", NATURE,GB,MACMILLAN JOURNALS LTD. LONDON, vol. 383, 31 October 1996 (1996-10-31), pages 804 - 806, XP000877459, ISSN: 0028-0836 *
NEIL M A A ET AL: "Real time 3D fluorescence microscopy by two beam interference illumination", OPTICS COMMUNICATIONS,NL,NORTH-HOLLAND PUBLISHING CO. AMSTERDAM, vol. 153, no. 1-3, 15 July 1998 (1998-07-15), pages 1 - 4, XP004146317, ISSN: 0030-4018 *

Also Published As

Publication number Publication date
WO2001044852A2 (en) 2001-06-21
DE19960583A1 (en) 2001-07-05
DE20023019U1 (en) 2002-11-07

Similar Documents

Publication Publication Date Title
DE69720458D1 (en) Programmable spatially light-modulated microscope and microscopy method
CA2351086A1 (en) High contrast, low distortion optical acquisition system for image capturing
DK1267707T3 (en) Method and system for characterization and cartography of tissue lesions
CA2263360A1 (en) Fingerprint imaging apparatus with auxiliary lens
AU5672296A (en) Method of observing objects under low levels of illumination and a device for carrying out the said method
DE60133916D1 (en) OPTICAL CONFOCUS SENSING DEVICE
DE60023156D1 (en) METHOD AND DEVICE FOR REDUCING THE TRAPEZER DISTORTION AND IMPROVING THE IMAGE DYE IN AN OPTICAL IMAGE ASSEMBLY SYSTEM
DE69802514D1 (en) IMAGING SYSTEM AND METHOD FOR MICROSCOPY
TW200626887A (en) Optical inspection of test surfaces
CA2246698A1 (en) Confocal microscope
WO1998010371A3 (en) Image sensor
EP0997768A3 (en) Illumination device and projection type display apparatus
CA2089079A1 (en) Machine vision surface characterization system
ATE304184T1 (en) IMAGING METHOD AND APPARATUS WITH LIGHT GUIDE BUNDLE AND SPATIAL LIGHT MODULATOR
WO2006030430A3 (en) Microscope system and method
WO2003025656A1 (en) Digital control scanning method and apparatus
WO2001044852A3 (en) Microscopy method and device
AU2003221505A1 (en) Sample carrier comprising integrated optics
DK1051660T3 (en) Apparatus as well as the method of capturing a subject
WO1997015855A3 (en) Optical instrument
US20130162801A1 (en) Microscope
US20040145804A1 (en) Microscope lens and the use of a microscope lens of this type in a microscope
CA2210801A1 (en) Method and apparatus for three-dimensional microscopy with enhanced depth resolution
FR2807830B1 (en) OPTOELECTRONIC THREE-DIMENSIONAL SHAPE ACQUISITION DEVICE
ATE425451T1 (en) ENGINE BEARING INSPECTION SYSTEM

Legal Events

Date Code Title Description
AK Designated states

Kind code of ref document: A2

Designated state(s): JP US

AL Designated countries for regional patents

Kind code of ref document: A2

Designated state(s): AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE TR

121 Ep: the epo has been informed by wipo that ep was designated in this application
DFPE Request for preliminary examination filed prior to expiration of 19th month from priority date (pct application filed before 20040101)
AK Designated states

Kind code of ref document: A3

Designated state(s): JP US

AL Designated countries for regional patents

Kind code of ref document: A3

Designated state(s): AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE TR

WWE Wipo information: entry into national phase

Ref document number: 2000989939

Country of ref document: EP

DFPE Request for preliminary examination filed prior to expiration of 19th month from priority date (pct application filed before 20040101)
WWW Wipo information: withdrawn in national office

Ref document number: 2000989939

Country of ref document: EP

122 Ep: pct application non-entry in european phase
NENP Non-entry into the national phase

Ref country code: JP