[go: up one dir, main page]
More Web Proxy on the site http://driver.im/

WO2000037663A3 - Plant transformation process - Google Patents

Plant transformation process Download PDF

Info

Publication number
WO2000037663A3
WO2000037663A3 PCT/US1999/030972 US9930972W WO0037663A3 WO 2000037663 A3 WO2000037663 A3 WO 2000037663A3 US 9930972 W US9930972 W US 9930972W WO 0037663 A3 WO0037663 A3 WO 0037663A3
Authority
WO
WIPO (PCT)
Prior art keywords
agrobacterium
seedlings
transgene
plant transformation
transformation process
Prior art date
Application number
PCT/US1999/030972
Other languages
French (fr)
Other versions
WO2000037663A2 (en
Inventor
Maria J Harrison
Anthony T Trieu
Original Assignee
Samuel Roberts Noble Found Inc
Maria J Harrison
Anthony T Trieu
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Samuel Roberts Noble Found Inc, Maria J Harrison, Anthony T Trieu filed Critical Samuel Roberts Noble Found Inc
Priority to AU25943/00A priority Critical patent/AU2594300A/en
Priority to CA002352488A priority patent/CA2352488A1/en
Priority to EP99968550A priority patent/EP1141356A2/en
Priority to JP2000589717A priority patent/JP2002533090A/en
Publication of WO2000037663A2 publication Critical patent/WO2000037663A2/en
Publication of WO2000037663A3 publication Critical patent/WO2000037663A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8202—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by biological means, e.g. cell mediated or natural vector
    • C12N15/8205—Agrobacterium mediated transformation

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Cell Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

A method of Agrobacterium-mediated genetic transformation using seedlings has been found which is applicable to dicots and monocots capable of being transformed by Agrobacterium. The transformation method utilizes vacuum-infiltration to introduce the Agrobacterium T-DNA carrying a gene of interest into the seedlings. Upon maturity, seeds collected from the infiltrated seedlings are germinated, and progeny carrying the transgene are selected. This transformation method produces progeny exhibiting stable inheritance of the transgene without the need for regeneration methods such as somatic embryogenesis or organogenesis.
PCT/US1999/030972 1998-12-23 1999-12-23 Plant transformation process WO2000037663A2 (en)

Priority Applications (4)

Application Number Priority Date Filing Date Title
AU25943/00A AU2594300A (en) 1998-12-23 1999-12-23 Plant transformation process
CA002352488A CA2352488A1 (en) 1998-12-23 1999-12-23 Plant transformation process
EP99968550A EP1141356A2 (en) 1998-12-23 1999-12-23 Plant transformation process
JP2000589717A JP2002533090A (en) 1998-12-23 1999-12-23 Plant transformation method

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US11371798P 1998-12-23 1998-12-23
US60/113,717 1998-12-23
US14537399P 1999-07-23 1999-07-23
US60/145,373 1999-07-23

Publications (2)

Publication Number Publication Date
WO2000037663A2 WO2000037663A2 (en) 2000-06-29
WO2000037663A3 true WO2000037663A3 (en) 2000-11-09

Family

ID=26811389

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1999/030972 WO2000037663A2 (en) 1998-12-23 1999-12-23 Plant transformation process

Country Status (5)

Country Link
EP (1) EP1141356A2 (en)
JP (1) JP2002533090A (en)
AU (1) AU2594300A (en)
CA (1) CA2352488A1 (en)
WO (1) WO2000037663A2 (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9574201B2 (en) 2010-06-09 2017-02-21 Bayer Cropscience Nv Methods and means to modify a plant genome at a nucleotide sequence commonly used in plant genome engineering

Families Citing this family (27)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2277592T3 (en) * 2000-08-03 2007-07-16 Japan Tobacco Inc. METHOD FOR STIMULATING THE EFFECTIVENESS OF INTRODUCTION OF GENES IN VEGETABLE CELLS.
CN1273604C (en) * 2000-08-03 2006-09-06 日本烟草产业株式会社 Method of improving gene transfer efficiency into plant cells
CN1149918C (en) * 2001-02-26 2004-05-19 山西省农业生物技术研究中心 Method for transferring agrobacterium mediated plant germination seed gene
KR100859715B1 (en) * 2002-07-16 2008-09-23 독립행정법인농업생물자원연구소 Device for electroporation including the use of depressurization/pressurization
JP5554414B2 (en) 2009-09-22 2014-07-23 メディカゴ インコーポレイテッド Method for preparing plant-derived VLPs
MX2012007681A (en) 2009-12-31 2013-01-29 Pioneer Hi Bred Int Engineering plant resistance to diseases caused by pathogens.
MY180803A (en) 2010-06-02 2020-12-09 Evolva Inc Recombinant production of steviol glycosides
EP2635684B1 (en) 2010-11-04 2016-10-26 Medicago Inc. Plant expression system
EP2655626B1 (en) * 2010-12-22 2018-04-11 Philip Morris Products S.a.s. Method and system for the vacuum infiltration of plants
TWI620816B (en) 2011-03-23 2018-04-11 苜蓿股份有限公司 Method of recovering plant-derived proteins
CN102212553A (en) * 2011-04-15 2011-10-12 山西省农业科学院生物技术研究中心 Ultrasonic-assisted agrobacterium-mediated plant germination seed gene transformation method
SG10201606565XA (en) 2011-08-08 2016-10-28 Evolva Sa Recombinant production of steviol glycosides
US9957540B2 (en) 2013-02-06 2018-05-01 Evolva Sa Methods for improved production of Rebaudioside D and Rebaudioside M
CA2900882A1 (en) 2013-02-11 2014-08-14 Evolva Sa Efficient production of steviol glycosides in recombinants hosts
ES2912291T3 (en) 2014-03-27 2022-05-25 Medicago Inc Modified CPMV Enhancer Items
JP6788582B2 (en) 2014-07-11 2020-11-25 メディカゴ インコーポレイテッド Modification of protein production in plants
US10421983B2 (en) 2014-08-11 2019-09-24 Evolva Sa Production of steviol glycosides in recombinant hosts
WO2016038095A2 (en) 2014-09-09 2016-03-17 Evolva Sa Production of steviol glycosides in recombinant hosts
CA2973674A1 (en) 2015-01-30 2016-08-04 Evolva Sa Production of steviol glycosides in recombinant hosts
CN115851470A (en) 2015-03-16 2023-03-28 帝斯曼知识产权资产管理有限公司 UDP-glycosyltransferase
MX2017016366A (en) 2015-07-02 2018-05-22 Medicago Inc Jasmonic acid pathway activator.
AU2016307066A1 (en) 2015-08-07 2018-02-08 Evolva Sa Production of steviol glycosides in recombinant hosts
JP2019513392A (en) 2016-04-13 2019-05-30 エヴォルヴァ エスアー．Ｅｖｏｌｖａ Ｓａ． Production of steviol glycosides in recombinant hosts
EP3458599A1 (en) 2016-05-16 2019-03-27 Evolva SA Production of steviol glycosides in recombinant hosts
WO2018083338A1 (en) 2016-11-07 2018-05-11 Evolva Sa Production of steviol glycosides in recombinant hosts
US20220396803A1 (en) 2021-06-09 2022-12-15 Nant Holdings Ip, Llc Methods And Systems For Producing A Protein Of Interest In A Plant
CN114657206B (en) * 2022-03-17 2024-03-22 中国热带农业科学院热带作物品种资源研究所 Genetic transformation method of stylosanthes guianensis

Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1989005859A1 (en) * 1987-12-21 1989-06-29 The Upjohn Company Agrobacterium mediated transformation of germinating plant seeds
WO1994002620A2 (en) * 1992-07-27 1994-02-03 Pioneer Hi-Bred International, Inc. An improved method of agrobacterium-mediated transformation of cultured soybean cells
WO1995028423A1 (en) * 1994-04-13 1995-10-26 The General Hospital Corporation Rps gene family, primers, probes, and detection methods
US5569597A (en) * 1985-05-13 1996-10-29 Ciba Geigy Corp. Methods of inserting viral DNA into plant material
WO1997048814A2 (en) * 1996-06-21 1997-12-24 Monsanto Company Methods for the production of stably-transformed, fertile wheat employing agrobacterium-mediated transformation and compositions derived therefrom
WO1998013503A1 (en) * 1996-09-23 1998-04-02 F.B. Investments Pty. Ltd. A plant and method of modification
WO2000022149A1 (en) * 1998-10-15 2000-04-20 Protein Research Trust Transformation process

Patent Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5569597A (en) * 1985-05-13 1996-10-29 Ciba Geigy Corp. Methods of inserting viral DNA into plant material
WO1989005859A1 (en) * 1987-12-21 1989-06-29 The Upjohn Company Agrobacterium mediated transformation of germinating plant seeds
WO1994002620A2 (en) * 1992-07-27 1994-02-03 Pioneer Hi-Bred International, Inc. An improved method of agrobacterium-mediated transformation of cultured soybean cells
WO1995028423A1 (en) * 1994-04-13 1995-10-26 The General Hospital Corporation Rps gene family, primers, probes, and detection methods
WO1997048814A2 (en) * 1996-06-21 1997-12-24 Monsanto Company Methods for the production of stably-transformed, fertile wheat employing agrobacterium-mediated transformation and compositions derived therefrom
WO1998013503A1 (en) * 1996-09-23 1998-04-02 F.B. Investments Pty. Ltd. A plant and method of modification
WO2000022149A1 (en) * 1998-10-15 2000-04-20 Protein Research Trust Transformation process

Non-Patent Citations (11)

* Cited by examiner, † Cited by third party
Title
BECHTOLD NICOLE ET AL: "In planta Agrobacterium mediated gene transfer by infiltration of adult Arabidopsis thaliana plants.", COMPTES RENDUS DE L'ACADEMIE DES SCIENCES SERIE III SCIENCES DE LA VIE 1993, vol. 316, no. 10, 1993, pages 1194 - 1199, XP000907460, ISSN: 0764-4469 *
BIOLOGIA PLANTARUM (PRAGUE) 1997-1998, vol. 40, no. 1, 1997, pages 33 - 41, ISSN: 0006-3134 *
CHEE P P ET AL: "TRANSFORMATION OF SOYBEAN GLYCINE-MAX BY INFECTING GERMINATING SEEDS WITH AGROBACTERIUM-TUMEFACIENS", PLANT PHYSIOLOGY (BETHESDA) 1989, vol. 91, no. 3, 1989, pages 1212 - 1218, XP002140336, ISSN: 0032-0889 *
DANHOF LINDA R ET AL: "Transformation of Arabidopsis by vacuum infiltration: A demonstration.", ANNUAL MEETING OF THE AMERICAN SOCIETY OF PLANT PHYSIOLOGISTS;SAN ANTONIO, TEXAS, USA; JULY 27-31, 1996, vol. 111, no. 2 SUPPL., 1996, Plant Physiology (Rockville) 1996, pages 164, XP002140332, ISSN: 0032-0889 *
DATABASE BIOSIS [online] BIOSCIENCES INFORMATION SERVICE, PHILADELPHIA, PA, US; 1995, BECHTOLD N ET AL: "In planta Agrobacterium-mediated transformation of adult Arabidopsis thaliana plants by vacuum infiltration.", XP002140338, Database accession no. PREV199698573015 *
DATABASE BIOSIS [online] BIOSCIENCES INFORMATION SERVICE, PHILADELPHIA, PA, US; 1996, TRIEU ANTHONY T ET AL: "Rapid transformation of Medicago truncatula: Regeneration via shoot organogenesis.", XP002140339, Database accession no. PREV199799329039 *
DATABASE BIOSIS [online] BIOSCIENCES INFORMATION SERVICE, PHILADELPHIA, PA, US; 1997, RAKOUSKY S ET AL: "Transient beta-glucuronidase activity after infiltration of Arabidopsis thaliana by Agrobacterium tumefaciens.", XP002140337, Database accession no. PREV199800165996 *
GENE TRANSFER TO PLANTS. 1995 SPRINGER-VERLAG;SPRINGER-VERLAG NEW YORK, INC. HEIDELBERGER PLATZ 3, D-1000 BERLIN, GERMANY; 175 FIFTH AVENUE, NEW YORK, NEW YORK 10010, USA, 1995, pages 19 - 23, ISBN: 3-540-58406-4 *
PLANT CELL REPORTS 1996, vol. 16, no. 1-2, 1996, pages 6 - 11, ISSN: 0721-7714 *
TRIEU ANTHONY T ET AL: "Approaches to improve the efficiency of transformation and regeneration of Medicago truncatula.", ANNUAL MEETING OF THE AMERICAN SOCIETY OF PLANT PHYSIOLOGISTS;CHARLOTTE, NORTH CAROLINA, USA; JULY 29-AUGUST 2, 1995, vol. 108, no. 2 SUPPL., 1995, Plant Physiology (Rockville) 1995, pages 57, XP002140335, ISSN: 0032-0889 *
WENCK ALLAN ET AL: "Frequent collinear long transfer of DNA inclusive of the whole binary vector during Agrobacterium-mediated transformation.", PLANT MOLECULAR BIOLOGY 1997, vol. 34, no. 6, 1997, pages 913 - 922, XP002140334, ISSN: 0167-4412 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9574201B2 (en) 2010-06-09 2017-02-21 Bayer Cropscience Nv Methods and means to modify a plant genome at a nucleotide sequence commonly used in plant genome engineering

Also Published As

Publication number Publication date
EP1141356A2 (en) 2001-10-10
JP2002533090A (en) 2002-10-08
CA2352488A1 (en) 2000-06-29
WO2000037663A2 (en) 2000-06-29
AU2594300A (en) 2000-07-12

Similar Documents

Publication Publication Date Title
WO2000037663A3 (en) Plant transformation process
WO2000063400A3 (en) Plant transformation process
KR102461443B1 (en) Methods and compositions for improving plant traits
WO1998049332A8 (en) Agrobacterium mediated transformation of sorghum
Manders et al. Transformation of passionfruit (Passiflora edulis fv flavicarpa Degener.) using Agrobacterium tumefaciens
WO2003003816A3 (en) Methods for enhancing segregation of transgenes in plants and compositions thereof
WO2000012735A3 (en) Transgene assay using stable agrobacterium rhizogenes transformation
Sirohi et al. CRISPR/Cas9 system: A potential tool for genetic improvement in floricultural crops
WO2001059086A3 (en) Methods and constructs for agrobacterium-mediated plant transformation
Bao et al. CRISPR/Cas9-based gene editing in soybean
GB9321183D0 (en) A method of plant transformation
CA2294211A1 (en) Method of increasing fruit size in a plant
AU4570397A (en) Transgenic lemnaceae
Song et al. Far‐red light inhibits lateral bud growth mainly through enhancing apical dominance independently of strigolactone synthesis in tomato
JP2022505440A (en) Dual guide RNA for CRISPR / Cas genome editing in plant cells
CN114277036A (en) Duck grass vernalization gene DgPAPS4 and application thereof
Rafsanjani et al. Transformation efficiency of calcium phosphate nanoparticles for genetic manipulation of Cichorium intybus L.
CA2411649A1 (en) Ires enabled gene trapping in plants
Fladung Xylem-specific overexpression of the GIBBERELLIN ACID 20 OXIDASE gene (GA20-OXIDASE) from pine in hybrid poplar (Populus tremula L.× P. alba L.) revealed reliable increase in growth and biomass production just in a single-copy-line
Parks et al. Composite plants for a composite plant: an efficient protocol for root studies in the sunflower using composite plants approach
CA2361143A1 (en) Transformation and regeneration of allium plants
WO2004035731A3 (en) Increasing host plant susceptibility to agrobacterium infection by overexpression of the arabidopsis vip1 gene
AU5213699A (en) (agrobacterium)-mediated transformation of turfgrass
DE10115761A1 (en) Preparing transgenic monocotyledonous plants for producing cereals, comprises timing the stages for optimal proliferative and regenerative capacity without use of marker DNA
CA2406820A1 (en) Improvement of flower morphology of plants by targeting mads-box gene

Legal Events

Date Code Title Description
AK Designated states

Kind code of ref document: A2

Designated state(s): AE AL AM AT AU AZ BA BB BG BR BY CA CH CN CR CU CZ DE DK DM EE ES FI GB GD GE GH GM HR HU ID IL IN IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MA MD MG MK MN MW MX NO NZ PL PT RO RU SD SE SG SI SK SL TJ TM TR TT TZ UA UG US UZ VN YU ZA ZW

AL Designated countries for regional patents

Kind code of ref document: A2

Designated state(s): GH GM KE LS MW SD SL SZ TZ UG ZW AM AZ BY KG KZ MD RU TJ TM AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE BF BJ CF CG CI CM GA GN GW ML MR NE SN TD TG

121 Ep: the epo has been informed by wipo that ep was designated in this application
DFPE Request for preliminary examination filed prior to expiration of 19th month from priority date (pct application filed before 20040101)

Free format text: (EXCEPT US)

AK Designated states

Kind code of ref document: A3

Designated state(s): AE AL AM AT AU AZ BA BB BG BR BY CA CH CN CR CU CZ DE DK DM EE ES FI GB GD GE GH GM HR HU ID IL IN IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MA MD MG MK MN MW MX NO NZ PL PT RO RU SD SE SG SI SK SL TJ TM TR TT TZ UA UG US UZ VN YU ZA ZW

AL Designated countries for regional patents

Kind code of ref document: A3

Designated state(s): GH GM KE LS MW SD SL SZ TZ UG ZW AM AZ BY KG KZ MD RU TJ TM AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE BF BJ CF CG CI CM GA GN GW ML MR NE SN TD TG

WWE Wipo information: entry into national phase

Ref document number: 09601979

Country of ref document: US

ENP Entry into the national phase

Ref document number: 2352488

Country of ref document: CA

Ref country code: CA

Ref document number: 2352488

Kind code of ref document: A

Format of ref document f/p: F

WWE Wipo information: entry into national phase

Ref document number: 512252

Country of ref document: NZ

ENP Entry into the national phase

Ref country code: JP

Ref document number: 2000 589717

Kind code of ref document: A

Format of ref document f/p: F

WWE Wipo information: entry into national phase

Ref document number: PA/a/2001/006531

Country of ref document: MX

WWE Wipo information: entry into national phase

Ref document number: 25943/00

Country of ref document: AU

WWE Wipo information: entry into national phase

Ref document number: 1999968550

Country of ref document: EP

WWP Wipo information: published in national office

Ref document number: 1999968550

Country of ref document: EP

REG Reference to national code

Ref country code: DE

Ref legal event code: 8642

WWW Wipo information: withdrawn in national office

Ref document number: 1999968550

Country of ref document: EP