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WO1999038986A3 - Riboflavin biosynthesis genes from plants and uses thereof - Google Patents

Riboflavin biosynthesis genes from plants and uses thereof Download PDF

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Publication number
WO1999038986A3
WO1999038986A3 PCT/EP1999/000556 EP9900556W WO9938986A3 WO 1999038986 A3 WO1999038986 A3 WO 1999038986A3 EP 9900556 W EP9900556 W EP 9900556W WO 9938986 A3 WO9938986 A3 WO 9938986A3
Authority
WO
WIPO (PCT)
Prior art keywords
plant
riboflavin biosynthesis
biosynthesis genes
riboflavin
synthase
Prior art date
Application number
PCT/EP1999/000556
Other languages
French (fr)
Other versions
WO1999038986A2 (en
Inventor
Charles David Guyer
Marie Ann Johnson
Sandra Lynn Volrath
Sandra Alice Brunn
Eric Russell Ward
Original Assignee
Novartis Ag
Novartis Erfind Verwalt Gmbh
Charles David Guyer
Marie Ann Johnson
Sandra Lynn Volrath
Sandra Alice Brunn
Eric Russell Ward
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Novartis Ag, Novartis Erfind Verwalt Gmbh, Charles David Guyer, Marie Ann Johnson, Sandra Lynn Volrath, Sandra Alice Brunn, Eric Russell Ward filed Critical Novartis Ag
Priority to BR9908213-6A priority Critical patent/BR9908213A/en
Priority to AU27202/99A priority patent/AU744487B2/en
Priority to IL13730399A priority patent/IL137303A0/en
Priority to HU0101278A priority patent/HUP0101278A3/en
Priority to CA002318522A priority patent/CA2318522A1/en
Priority to PL99342805A priority patent/PL342805A1/en
Priority to EP99907444A priority patent/EP1051504A2/en
Priority to JP2000529444A priority patent/JP2002501753A/en
Publication of WO1999038986A2 publication Critical patent/WO1999038986A2/en
Publication of WO1999038986A3 publication Critical patent/WO1999038986A3/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1085—Transferases (2.) transferring alkyl or aryl groups other than methyl groups (2.5)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8274—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for herbicide resistance
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Nutrition Science (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

The present invention provides plant riboflavin biosynthesis genes, including a gene that encodes the β subunit of the plant riboflavin synthase enzyme complex (lumazine synthase) and a gene that encodes the bifunctional enzyme GTP cyclohydrolase II/DHBP synthase. Also disclosed are the recombinant production of these plant riboflavin biosynthesis enzymes in heterologous hosts, screening chemicals for herbicidal activity using these recombinantly produced enzymes, and the use of thereby identified herbicidal chemicals to suppress the growth of undesired vegetation. Furthermore, the present invention provides methods for the development of herbicide tolerance in plants, plant tissues, plant seeds and plant cells using the riboflavin biosynthesis genes of the invention.
PCT/EP1999/000556 1998-01-30 1999-01-28 Riboflavin biosynthesis genes from plants and uses thereof WO1999038986A2 (en)

Priority Applications (8)

Application Number Priority Date Filing Date Title
BR9908213-6A BR9908213A (en) 1998-01-30 1999-01-28 Genes of riboflavin biosynthesis from plants and their uses
AU27202/99A AU744487B2 (en) 1998-01-30 1999-01-28 Riboflavin biosynthesis genes from plants and uses thereof
IL13730399A IL137303A0 (en) 1998-01-30 1999-01-28 Ribloflavin biosynthesis genes from plants and uses thereof
HU0101278A HUP0101278A3 (en) 1998-01-30 1999-01-28 Riboflavin biosynthesis genes from plants and uses thereof
CA002318522A CA2318522A1 (en) 1998-01-30 1999-01-28 Riboflavin biosynthesis genes from plants and uses thereof
PL99342805A PL342805A1 (en) 1998-01-30 1999-01-28 Genes of riboflavin biosynthesis from plants and their applications
EP99907444A EP1051504A2 (en) 1998-01-30 1999-01-28 Riboflavin biosynthesis genes from plants and uses thereof
JP2000529444A JP2002501753A (en) 1998-01-30 1999-01-28 Riboflavin biosynthesis genes from plants and uses thereof

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US10981098P 1998-01-30 1998-01-30
US60/109,810 1998-01-30

Publications (2)

Publication Number Publication Date
WO1999038986A2 WO1999038986A2 (en) 1999-08-05
WO1999038986A3 true WO1999038986A3 (en) 1999-09-23

Family

ID=22329685

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP1999/000556 WO1999038986A2 (en) 1998-01-30 1999-01-28 Riboflavin biosynthesis genes from plants and uses thereof

Country Status (17)

Country Link
EP (1) EP1051504A2 (en)
JP (1) JP2002501753A (en)
KR (1) KR20010082509A (en)
CN (1) CN1289369A (en)
AU (1) AU744487B2 (en)
BR (1) BR9908213A (en)
CA (1) CA2318522A1 (en)
CZ (1) CZ20002750A3 (en)
HU (1) HUP0101278A3 (en)
ID (1) ID25910A (en)
IL (1) IL137303A0 (en)
IN (1) IN2000CH00209A (en)
MX (1) MXPA00007432A (en)
PL (1) PL342805A1 (en)
TR (1) TR200002193T2 (en)
WO (1) WO1999038986A2 (en)
ZA (1) ZA99716B (en)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
IL140442A0 (en) * 1998-08-12 2002-02-10 Maxygen Inc Dna shuffling to produce herbicide selective crops
WO2000040744A1 (en) * 1998-12-15 2000-07-13 Adelbert Bacher Method for screening for inhibitors of riboflavin biosynthesis
DE19910102B4 (en) * 1999-03-08 2006-06-01 Fischer, Markus, Dr.rer.nat. Protein conjugates, methods, vectors, proteins and DNA for their production, their use, and medicines and vaccines containing the same
DE19942175A1 (en) * 1999-09-03 2001-03-08 Adelbert Bacher Method for finding inhibitors of riboflavin biosynthesis
EP1276883A2 (en) * 2000-04-18 2003-01-22 Syngenta Participations AG Amino acid:glyoxylate aminotransferase genes from plants and uses thereof
CN110964769B (en) * 2019-11-29 2022-11-11 河南巨龙生物工程股份有限公司 Method for improving yield of riboflavin produced by fermenting bacillus subtilis

Non-Patent Citations (6)

* Cited by examiner, † Cited by third party
Title
DE LOO, F.J., ET AL .: "expressed sequence tags from developing castor seeds", EMBL SEQUENCE DATA LIBRARY, 18 March 1995 (1995-03-18), heidelberg, germany, XP002110953 *
DEPREZ,T., ET AL.: "The Arabidopsis thaliana transcribed genome: the GDR cDNA program", EMBL SEQUENCE DATA LIBRARY, 6 June 1994 (1994-06-06), heidelberg, germany, XP002110952 *
HERZ, S.W., ET AL.: "biosynthesis of riboflavin in plants. The ribA gene of Arabidopsis thaliana specifies a bifunctional GTP cyclohydrolase II/3,4-dihydroxy-2butanone-4-phosphate synthase - unpublished", EMBL SEQUENCE DATA LIBRARY, 1 October 1997 (1997-10-01), heidelberg, germany, XP002110950 *
HERZ, S.W., ET AL.: "unpublished", EMBL SEQUENCE DATA LIBRARY, 29 October 1997 (1997-10-29), HEIDELBERG, GERMANY, XP002110951 *
KOBAYASHI,M., ET AL.: "isolation of cDNAs encoding GTP cyclohydrolase II from Arabidopsis thaliana", GENE, vol. 160, 1995, pages 303 - 304, XP002110949 *
STEMMER W P C: "RAPID EVOLUTION OF A PROTEIN IN VITRO BY DNA SHUFFLING", NATURE, vol. 370, 4 August 1994 (1994-08-04), pages 389 - 391, XP002912216, ISSN: 0028-0836 *

Also Published As

Publication number Publication date
WO1999038986A2 (en) 1999-08-05
ZA99716B (en) 1999-07-30
JP2002501753A (en) 2002-01-22
MXPA00007432A (en) 2001-02-01
BR9908213A (en) 2000-11-28
CZ20002750A3 (en) 2000-11-15
HUP0101278A2 (en) 2001-08-28
AU744487B2 (en) 2002-02-28
EP1051504A2 (en) 2000-11-15
IL137303A0 (en) 2001-07-24
KR20010082509A (en) 2001-08-30
ID25910A (en) 2000-11-09
CA2318522A1 (en) 1999-08-05
AU2720299A (en) 1999-08-16
IN2000CH00209A (en) 2005-05-20
TR200002193T2 (en) 2001-05-21
HUP0101278A3 (en) 2003-04-28
CN1289369A (en) 2001-03-28
PL342805A1 (en) 2001-07-02

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