WO1997009958A1

WO1997009958A1 - Cosmetic

Info

Publication number: WO1997009958A1
Application number: PCT/JP1996/002645
Authority: WO
Inventors: Nobuchika Urakabe; Keiichiro Okabe
Original assignee: Kabushiki Kaisya Advance
Priority date: 1995-09-14
Filing date: 1996-09-13
Publication date: 1997-03-20
Also published as: AU6945596A; JPH0977653A

Abstract

A cosmetic composition which is efficacious in normalizing the skin and protecting the skin from damages due to active oxygen and ultraviolet rays, thus solving the problems encountering in the conventional cosmetics. The composition contains amino acids or higher fatty acids essentially required by indigenous dermal bacteria as a proliferation factor for these bacteria, higher fatty acids as a growth inhibiting factor for noxious dermal bacteria, sebum or sweat components as a nutrient for indigenous dermal bacteria, and a base. The cosmetic can supply a 'sebaceous layer' similar to the natural one composed of various normal dermal bacteria and thus make the skin beautiful and healthy.

Description

Description and Cosmetic Technology

The present invention relates to a cosmetic composition, and more particularly to a normal human skin comprising one or more of a growth factor of a resident bacteria, a growth inhibitor of a skin harmful bacterium, and a nutrient of the resident bacteria. The present invention relates to a cosmetic composition which is effective for protecting the skin from damage caused by active oxygen and ultraviolet rays. Background art

Conventionally, cosmetics aimed at preventing or improving the dryness and roughness of the skin and protecting against damage by active oxygen and ultraviolet rays have the skin protective and self-cleansing effects of human sebum film such as cream. Simulated materials, those with skin activating effects containing raw materials extracted from natural sources, and those that protect skin from damage caused by ultraviolet rays containing materials that reflect or absorb ultraviolet rays have been actively used. I have.

However, artificially formulated cosmetics are far and far from those that are formulated by the natural function of the skin, and human skin has normal, greasy and dry greasy skin. There are four types of skin, dry skin, and the methods for preventing and improving skin dryness and roughness must be changed for each type.

In addition, cosmetics artificially formulated for the purpose of protecting against damage by active oxygen or ultraviolet rays have a limited action time. Furthermore, artificially formulated cosmetics have many problems in terms of their stability when compared to the situation where they are formed by the natural function of the skin. Therefore, the conventional cosmetics are used to normalize the skin, It has the disadvantage that a wide range of effects and security cannot be expected for the protection of obstacles. Disclosure of the invention

Therefore, an object of the present invention is to solve the above-mentioned problems of the conventional cosmetics and to provide a cosmetic composition effective for normalizing the skin J and protecting against damage by active oxygen and ultraviolet rays.

According to the present invention, it comprises one or more factors or components selected from among growth factors of skin-resident bacteria, growth-inhibiting factors of skin-harmful bacteria and nutrients of skin-resident bacteria, and a base. Provided is a cosmetic composition. BRIEF DESCRIPTION OF THE FIGURES

Hereinafter, the present invention will be described more specifically with reference to the drawings.

FIGS. 1 (a), (b) and (c) are graphs showing the effect of keratin or olive oil-containing medium on the growth of skin-resident bacteria.

FIG. 2 is a graph showing the effect of stearic acid and oleic acid on the growth of bacteria indigenous to skin.

FIGS. 3 (a) and 3 (b) are graphs showing the ultraviolet absorption spectrum of the cell component liquid and the sunscreen cream. BEST MODE FOR CARRYING OUT THE INVENTION

In view of the above situation, the present inventors have conducted intensive studies on a method that has a wide range of effects on skin normalization and protection against damage by active oxygen and ultraviolet rays. It has been clarified that normalization and further activation are important requirements.

Specifically, skin) Growth factors of resident bacteria, production of skin harmful bacteria Normalization and activity of the skin by spraying or applying to the skin a composition containing one or more factors or components selected from growth inhibitory factors and nutrients of the skin-resident bacteria The present inventors have recognized that the present invention has a remarkable effect on preventing damage caused by oxygen and ultraviolet rays, and have completed the present invention. Thus, the present invention is based on the finding that the present case of natural sebum membrane is nothing but skin resident bacteria and its metabolites, and skin resident bacteria are damaged by active oxygen and ultraviolet rays generated on the surface of skin I. This was done by paying attention to the defense.

That is, the cosmetic composition of the present invention comprises Propionibacterium acnes or Prophylactic bacterium acnes or Staphylococcus epidermidis. ) Such as arginine, isoleucine, leucine, methionine, phenylalanine, proline, and tripfa, which are essential amino acids as growth factors for skin-resident bacteria such as , Tyrosine, parin, and one or more of stearic acid and oleic acid, which are higher fatty acids.

In addition, the cosmetic composition of the present invention is characterized by containing a high fatty acid as a higher fatty acid as a growth inhibitory factor of skin harmful bacteria such as Staphylococcus aureus X (Staphylococcus aureus). It contains one or more acids, myristic acid, palmitoleic acid, linoleic acid, linolenic acid, arachidic acid, and arachidonic acid.

Furthermore, the cosmetic composition of the present invention contains one or more components contained in sebum and perspiration as a nutrient source of skin and resident bacteria.

The present inventors have conducted intensive studies on the number and type of bacteria resident on the skin, the components of the skin surface, the physiological and biochemical conditions of the skin, and the like for each age group of men and women. And elucidated.

Propionibacterium 'Aknes and Sphycococcus epidermidis are resident on the surface of human skin as typical bacteria. It was found that the average number of propionibacterial bacterium ranges from tens of thousands to hundreds of thousands and the number of Staphylococcus epidermidis ranges from hundreds to thousands per cm ^{2 of} skin.

These indigenous skin karada bacteria grow and repeat the growth using substances such as sebum and sweat, fats, proteins, and carbohydrates secreted or present in the skin surface layer as nutrient sources. At this time, bacteria indigenous to the skin produce a wide variety of metabolites, which emulsify sebum secreted from sebaceous glands and sweat secreted from sweat glands, and prevent drying and inflammation of the skin. It has been found that it protects against damage caused by ultraviolet rays and forms a layer that inhibits the growth of skin harmful bacteria (hereinafter referred to as the “sebum layer”).

With respect to the “sebum layer” constituted by the skin-resident bacteria, metabolites of the skin-resident bacteria, and substances present or secreted on the skin surface, the main functions that have been elucidated are as follows, for example.

(i) Emulsions of sebum and sweat and metabolites of skin frost-resident bacteria protect the skin from drying by suppressing the evaporation of water from the skin.

(ii) It acts as a barrier layer against chemical and physical obstacles constantly applied from the outside, protecting the skin from inflammation and damage.

(iii) Skin I Enzymes such as superoxide dismutase (SOD) and ribonuclease produced by indigenous bacteria protect skin from damage by eliminating the oxidizing power of active oxygen.

(iv) Skin-resident bacteria protect the skin from UV damage by directly absorbing UV light.

(V) Free fatty acids, which are produced by the metabolism of lipids by indigenous skin bacteria, mainly maintain the surface pH of the skin at a weak acidity (around pH 6) and inhibit the growth of skin harmful bacteria. Is involved in cleansing the skin by However, as a result of comparing and examining the data for men and women of each age group, the number of bacteria indigenous to the skin and the amount of sebum and sweat secreted in the surface layer of skin I with age increase as a result of the fact that the skin that maintains the healthy skin is healthy. It was found that the number of skin oils decreased and the “sebum layer” deteriorated, and that harmful bacteria in sebum multiplied and the healthy balance of skin flora was destroyed.

Therefore, one or more selected from among the decreasing growth factors of the indigenous skin bacteria and the growth inhibitory factors of the skin harmful bacteria, and at the same time, one or more of the sebum and sweat components, which are similarly reduced It was found that when the composition was blended into a composition and sprayed or applied, the roughened skin and inflammation were alleviated, and the skin was improved to be beautiful and healthy. Thus, the present invention was completed.

The mixing ratio (% by weight) of the growth factor of the resident bacteria to the cosmetic composition is in accordance with the natural composition of amino acids and higher fatty acids present in the surface layer of healthy skin. It is as follows.

Essential amino acids that are growth factors of skin-resident bacteria (arginine, isoleucine, leucine, methionine, phenylalanine, proline, tributphan, tyrosine, bali ), 0.01 to 10% by weight, more preferably 0.05 to 1% by weight, respectively, especially essential amino acid which is a growth factor of bacteria indigenous to the skin. As a group, keratin or the like containing all essential amino acids in a balanced manner except for tributphan can be used. If keratin is used, it is 0.01 to 50% by weight (more preferably 0.5 to 10% by weight).

When higher fatty acids (stearic acid and oleic acid), which are growth factors of skin-resident bacteria, are added, the amount is preferably 0.01 to 50% by weight, and more preferably 0.5 to 10%. % By weight.

The compounding ratio (% by weight) of the growth inhibitory factor of skin harmful bacteria to the cosmetic composition conforms to the natural composition of higher fatty acids present in the surface layer of healthy skin, and is usually as follows. Higher fatty acids (LA ゥ Phosphoric acid, myristic acid,. Luminoleic acid, linoleic acid, linolenic acid, arachidic acid, arachidonic acid) are each 0.05% by weight, more preferably 0.510% by weight. It is.

The proportion (% by weight) of the sebum and sweat components as a nutrient source of the resident bacteria in the cosmetics is based on the natural composition of the sebum and sweat components present in the surface layer of healthy skin I. But usually as follows

The components of sebum (triglyceride, wax ester, squalene, cholesterol ester, cholesterol) are each 0.050% by weight, more preferably 0.510% by weight. % By weight.

The components of sweat (water, chlorine, sodium, potassium, calcium, magnesium, nitrogen, ammonia, glucose, lactic acid) are each 0.0001 to 15% by weight, more preferably Is 0.052% by weight.

The above factors or components are listed in the Japanese Pharmacopoeia and the Standards for Cosmetic Ingredients, so their quality and standards conform to them.

The cosmetic composition of the present invention can contain, as a base and an additive, any components other than the above-described factors or components as long as the effects of the present invention are not impaired. Ingredients normally included in cosmetic compositions, such as ethanol, oily ingredients, humectants, thickeners, preservatives, emulsifiers, medicinal ingredients, powders, fragrances, emulsion stabilizers, pH adjusters, etc. can do.

Specifically, the oily components include liquid paraffin, petrolatum, raffin wax, skullane, mitsurou, carnauba baro, olive oil, and lano Examples include phosphorus, higher alcohols, fatty acids, synthetic ester oils of higher alcohols and fatty acids, and silicone oils, and humectants such as sorbitol, xylitol, and grease. Serine, multi-tool, propylene call, 1,3—butylene recall, 14 butylene Examples of the thickening agent include alcohol, sodium lipidone carboxylate, lactic acid, sodium lactate, polyoxypropylene fatty acid ester, and polyethylene glycol. Examples include water-soluble polymers such as ruboxyvinyl polymer, ruboxymethylcellulose, polyvinyl alcohol, carrageenan, and gelatin, and electrolytes such as sodium chloride and potassium chloride. Examples of emulsifying agents include urea, methyl paraben, ethyl paraben, propyl paraben, butyl paraben, sodium benzoate, and the like. Examples of emulsifying agents include polyoxyethylene alkyl ether, polyoxyethylene fatty acid ester, and polyoxyethylene. Sorbitan fatty acid ester, glycerin fatty acid ester, polyglycerin fatty acid ester And non-ionic surfactants such as polyoxyethylene glycol glycerin fatty acid ester, polyoxyethylene hydrogenated castor oil, and polyoxyethylene sorbitol fatty acid ethylene, and the like. Recital, My power, kaolin, silica, penite, vermiculite, zinc white, mica, mica titan, titanium chloride, magnesium oxide, zirconium oxide, barium sulfide, bengala

, Iron oxide, ultramarine and the like, and examples of the pH adjusting agent include buffering agents such as sodium lactic acid-lactate and sodium monocitrate. Also, various active ingredients include alantoin, vitamin E derivative, glycyrrhizin, ascorbic acid derivative, kojic acid, arbutin, and the like. Addition of an acetic acid derivative, a brassentia extract, an anti-inflammatory agent, quinine, various plant extracts, and the like can improve the melanin-suppressing effect. Furthermore, by adding various ultraviolet absorbing or reflecting substances, it is possible to obtain a cosmetic that enhances the preventive and therapeutic effects of sunburn.

In general, the compounding amount is, for example, 1 to ²⁰ mg per 1 cm2 of the skin surface in the case of a cream or ointment, and the same in the case of a liquid preparation. Preferably, the amount is up to 10 mg, but it is not limited to this. In addition, the cosmetic of the present invention can be used for makeup cosmetics such as cosmetics such as lotions, creams, emulsions, and packs, and foundations, lipsticks, and almost reds. It can be widely applied to cosmetics for hair, such as cosmetics, shampoos, rinses, and hair tonics, and other cosmetics.

Furthermore, the growth factor of the indigenous skin bacteria and the growth inhibitory factor of the skin-harmful bacteria according to the present invention can be applied not only to cosmetics but also to products in related fields such as various chemicals and drugs.

The cosmetics of the present invention, by virtue of the skin if bacterial flora that differs from one human to another, proliferate and activate skin Iff resident bacteria and inhibit the growth of skin harmful bacteria, and therefore, have a healthy skin flora. It can provide a "sebum layer" that is closer to nature than it is composed. In addition, it proliferates the skin flora specific to each human. ■ It is much safer than adding skin bacteria from the outside because it activates.

This “sebum layer” protects the skin from dryness, acts as a barrier layer against externally applied chemical and physical damage, protects against damage caused by active oxygen and ultraviolet rays, and further protects the skin. Good skin protection, such as inhibiting the growth of harmful bacteria. · Achieves self-cleansing, making skin beautiful and healthy. Example

Next, the present invention will be described with reference to Examples, but it goes without saying that the present invention is not limited to these Examples.

First, essential amino acids as growth factors of bacteria resident in the skin according to the present invention will be described.

Example 1 [Aminoacid requirement of skin-resident bacteria]

Propionibacterium akunes isolated from healthy adults (Hereinafter referred to as “Pa”).

(Propionibacterium granulosumJ ^ F "Pg" and when

Table 1 shows the results of examining the amino acid requirement of Kocani Papasginichitopu in the evening.

Table 1 Amino acid requirement of bacteria indigenous to skin

Pa Pg Se Amino acid KS81 NN48 Y067

E; Required (in Zena Mi Amino Acids containing medium 37, 48 - growth (0 D ₆₀ 0 value after 72 hr) carbonochloridate 25%)

Pa; Propionibacterium acnes, Pg: Propionibacterium granulosum, Se; Sufi Filococcus epidermidice, KS81, NN48, Y067 ; "Pa" in media containing strain code total a Mi Amino acids, Rpgj, and Te 37 "Se", was the growth rate at 72 hours 48 hours (0 D ₆ .. value) 100% In contrast, the growth rate when each amino acid was depleted was 25 %, The amino acid was defined as an essential amino acid. Based on the results of this experiment, essential amino acids as growth factors for skin-resident bacteria include arginine, isoleucine, leucine, methionine, phenylalanine, proline, and triline. Butphan, tyrosin, and balin were found. Methionine, phenylalanine, and tyrosin were essential for the genus Propionibacterium, and proline was essential for the genus Sukkoficus.

The other amino acids were essential for the genus Propionibatatum and Staphylococcus coccus II.

Example 2 [Effect of Essential Amino Acid on the Growth of Bacteria Resident in Skin Frost]

Keratin derived from human keratin is used as a growth factor source containing an essential amino acid as a growth factor of skin-resident bacteria in a well-balanced manner, and skin-resident bacteria `` Pa '', rpgj, And the proliferation effect of “Se” was confirmed. The amino acid composition of keratin derived from human keratin is shown in Table 2, which contains a balanced balance of essential amino acids of all skin-resident bacteria except for tributphan. o

Table 2 Amino acid composition of keratin derived from human keratin

Vachipuaisurofugumegirito 1 C

(Residue ziooo residue)

Evening skin

Job 22

There's 46

Fine runner 93

Five

Fungus Nin 34

Non 29

0

20

End 57

(Other amino acids)

Araninno 71

Spartic acid 98

/ 2 Sistine 29

Acid 158

116

N 16

57

72

In this example, the PYG broth medium (medium composition: g Z 1>; triptycase 1, yeast extract 0.2, glucose 2, NaCl 2.5, NaH ₂ P043, K2HPO47. Since cystine hydrochloride 0.2, pH 6.8) is used, the insufficient tributane is added to the medium with triptycase (containing 0.9% tributane) and yeast extract. (Containing 1.0% tributane). Fig. 1 shows the growth of each skin-resident bacterium when keratin was added to the medium and when keratin was not added (control). Fig. 1 (a) is a propionate battery, ATCC 11827 standard strain), and Fig. 1 (b) is a propionium powder, granulosum (collected from healthy individuals) NN48, Fig. 1 ( c) shows the case of Staphylococcus epidermidis Y067 (collected from healthy people). When keratin was added, “Pa”, rpgj, and “Se” showed a clear proliferation effect as compared with the case where keratin was not added. Also, skin resident thin Compared to the case of adding the same amount of olive oil, which is a nutrient source of bacteria,

Keratin exhibited an excellent growth-promoting effect on all the growths of “Pa”, “Pg”, and “Se”.

In addition, higher fatty acids as growth factors of skin-resident bacteria according to the present invention will be described.

Example 3 [Effects of stearic acid and oleic acid on the growth of bacteria resident on skin]

A typical skin-resident bacterium, “Paj standard bacterial propioni bacterium”, was obtained by adding gZml of various higher fatty acids to a PYG pros medium (the medium composition was the same as in Example 2) and culturing at 37 ° C for 48 hours. The growth results of ATCC 11827 are shown in Fig. 2. Among various higher fatty acids, stearic acid and oleic acid showed a clear “Pa” growth effect. Next, higher fatty acids as growth inhibitory factors for skin harmful bacteria according to the present invention will be described.

Example 4 (Effect of Higher Fatty Acid on Growth of Skin Harmful Bacteria)

GA agar plate to which various higher fatty acids are added stepwise from 5 mgZml to 0.010 mg / ml (in 74.0 g / 1; peptone 10.0, soybean peptone 3.0, proteose peptone 10.0, digested serum powder 13.5, yeast extract) 5.0, Meat Extract 2.2, Liver Extract Powder 1.2, Bactose Sugar 3.0, Cadmium Dihydrogen Phosphate 3.0, Sodium Chloride 3.0, Soluble Starch 5.0, L-cistine Hydrochloride 0.3, Nattothioglycolic Acid Li Umm 0.3, agar 15.0, to pH7.1), skin harmful, which is a typical bacterium bacteria scan evening _ I - Lock dregs * § window Reus (hereinafter referred to as "Sa" of) the field of 10 ⁸ Zml bacterial solution Table 3 shows the results of confirming the growth-inhibiting effect of the cells coated with a wire and cultured aerobically at 37 ° C for 24 hours. Table 3 Growth inhibitory effect of higher fatty acids on skin harmful bacteria

(1) Sufu 1 Loco, 2 Cas' Aureus NN47

1 2) Suco Rococcus' Aureus _67]

+ Multiplication-no multiplication

GAM agar plates to 10 ³ bacteria solution streaking smear, aerobic culture (37 ° C 24hr)

12: 0 laurate, 14: 0 Mi Risuchin acid, 16: 0 ⁰ palmitic acid,) 6: 1 see train acid, 18: 0 stearic phosphate, 18: 1 Orein acid, 18: 2 Reno one le acid, 18: 3 Linolenic acid,

200 arachidic acid, 20: 4 arachidonic acid Among various higher fatty acids, lauric acid, myristic acid, palmitoleic acid, linoleic acid, linolenic acid, arachidic acid, When arachidonic acid was 2.5 mg / ml or less, it exhibited the growth inhibitory effect of “Sa”. In particular, it inhibited growth at low concentrations against two strains, namely, rauric acid, palmitoleic acid and linolenic acid “Sa”. Furthermore, the sebum component as a nutrient source of the skin It resident bacteria according to the present invention

The growth effect of bacteria that are indigenous to captive skin when, and sweat components are added will be described.

Example 5 [Effects of sebum component and sweat component on skin-resident bacteria]

The sebum component and sweat component were added to the PYG broth medium (the medium composition was the same as in Example 2) at the ratios shown in Table 4, and the results were compared with the case where no sebum component and sweat component were added. The growth promotion rate of "Pa" (determined by the size of the colony) increased by about 20%, the growth promotion rate of "Se" increased by about 30%, and the growth of sebum and sweat components indigenous bacteria on skin The effect was shown.

Table 4 Sebum and sweat components

The growth effects and associated activation of the skin-resident bacteria described above provide a more natural `` sebum layer '' composed of healthy skin flora, protects the skin from drying, and adds external chemistry.・ Working as barrier layer against physical obstacles, preventing damage due to active oxygen and ultraviolet rays, and also good skin protection such as inhibiting the growth of skin harmful bacteria Achieves action and makes skin beautiful and healthy.

Skin protection of healthy skin bacteria flora · Among the self-cleansing effects, the most important protective effects against active oxygen and ultraviolet rays are described.

Reactive oxygen (〇 2 ―) etc. are used for peroxidation of lipids, gene cleavage, protein It is considered to be a cause of degeneration, etc., and is one of the factors that promote tissue damage and aging. In particular, the surface of the skin It is constantly exposed to the outside air, so it is in an environment where active oxygen is likely to be generated. It was shown that the indigenous skin bacteria, which normally inhabit and grow on the surface of the skin, scavenge active oxygen by enzymes such as superoxide dismutase (hereinafter referred to as SOD) and power tarase. o

Example 6 (Measurement of SOD activity of resident bacteria)

The SOD activity produced inside and outside the cells of “Pa” and “Se”, which are representatives of skin-resident bacteria, was measured. The SOD activity was measured by the NBT reduction method (Newborn Chemistry Laboratory Course 5, “Biooxidation and Drug Metabolism”, Tokyo Chemical Dojin; 1992). When NBT (Nitroble-tetrazolium) is added to a system in which xanthine is allowed to act on xanthine oxidase to constantly produce 0 _2- , the NBT is reduced by 〇 _2- and color is developed. Is measured by the absorbance at a wavelength of 560 nm, the concentration of O _2- can be measured. Therefore, in this experimental example, the SOD activity can be measured with a degree of decrease in the absorbance, as compared with the control containing no indigenous skin bacteria. Tables 5 and 6 show the extracellular SOD activity and the intracellular SOD activity actually measured with a standard strain of skin-resident bacteria. Table 5 ^ ¾¾¾ Extracellular cells at the target 戦 SOD ^:

Unit: IU / ml Staphylococcus · Epidermidi Propio-kuterium · Before cultivation Culture of IA 12012 ¾iijf Culture of funkness dish 11827 GAM supernatant 1

X 1/4 X 1/2 X 1

0.00 ± 0.08 0.14 ± 0.01 0.23 ± 0.09 0.48 ± 0.10 0.01 ± 0.06 6 Skin Activity of Intracellular SDS Extract of Indigenous Bacterial Standard Strain

“Se”, which grows on the skin surface, showed SOD activity outside and inside the cells. "Pa" which grows deeper than "Se" showed SOD activity in the cells. This indicates that “Se”, which grows on the skin surface affected by a large amount of reactive oxygen, produces and responds to SOD outside the cells.

Table 7 shows the results of measuring the SOD activity of 10 “Pa” strains and 9 “Se” strains isolated from healthy individuals.

Table 7 Rin Λ ^ ¾ ^ 0 Intracellular germ attack ¾WJ

(n = 3, once for each culture tube)

Lin code Recovered cells SOD extract of culture supernatant

MM. SOD activity (4 protein

(mg / τηme (IU / mg cells) (m / m £)

Kuteriumakunessu

10.5 ± 1.4 one 0.24 ± 0.08 1.37 ± 0.02 5.1 ± 0.6 8.0 ± 2.1 one 0.38 ± 0.28 0.35 ± 0.02 8.6 ± 0.7 6.9 ± 0.4 -0.23 ± 0.10 0.45 ± 0.02 12.0 ± 2.5 17.2 ± 0.8 one 0.16 ± 0.02 0.38 ± 0.08 28.8 ± 6.8 15.0 ± 1.5 one 0.16 ± 0.00 1.20 ± 0.17 5.1 ± 1.4 8.3 ± 1.1 — 0.10 ± 0.19 0.29 ± 0.09 15.9 ± 5.112.9 ± 1.0 0.09 ± 0.10 0.83 ± 0.07 6.9 ± 2.1 10.8 ± 0.7 one 0.17 ± 0.04 0.54 ± 0.06 11.7 ± 3 15.5 ± 6.6 1 0.14 ± 0.08 0.60 ± 0.34 13.8 ± 9.0 13.5 ± 1.4 1 0.15 ± 0.10 1.38 ± 0.11 5.7 ± 0.6

11.9 one 0.16 0.74 11.4

Cockasepidermidis

4.4 ± 0.3 1.06 ± 0.09 0.72 ± 0.16 81.9 ± 8.0 118 ± 33

4.9 ± 0.9 0.96 ± 0.35 0.40 ± 0.04 37.5 ± 1.9 94 ± 6

5.4 ± 0.4 0.27 ± 0.05 0.55 ± 0.06 12.4 ± 3.4 22 ± 6

5.3 ± 0 5 0.38 ± 0.17 0.63 ± 0.03 20.0 ± 7.9 32 ± 14

5.4 ± 0.4 0.60 ± 0.13 0.66 ± 0.03 29.2 ± 3.4 44 ± 6

5.6 ± 0.4 0.82 ± 0.18 0.75 ± 0.04 49.3 ± 8.9 66 ± 10

5.1 ± 1.8 3.30 ± 0.51 0.69 ± 0.02 32.2 ± 7.0 47 ± 11

4.9 ± 2.0 4.93 ± 0.26 0.86 ± 0.02 36.4 ± 1.2 42 ± 1

3.6 ± 1.0 8.24 ± 0.36 1.20 ± 0.30 594 ± 627 447 ± 427

5.0 2.28 0.72 99.2 101.3 "Se", which grows on the skin surface, showed about 10 times the SOD activity compared to "Pa", which grew deep in the skin surface. The SOD activity of “Pa” varied among individuals, with 5.1 IUZmg protein in low individuals and 28.8 IUZmg protein in high individuals. The SOD activity of “Se” was even more individualized, with 22 IUZmg protein in low individuals and 447 lUZmg protein in high individuals. Although there are individual differences, the SOD activity showed the same tendency as the standard strain.

Based on the above, it was found that skin-resident bacteria have SOD activity that eliminates active oxygen that damages the skin, and that there are individual differences (differences in strains). Significance of activation is shown Thus, it can be said that the growth and activation of the skin-resident bacteria according to the present invention have an effect of increasing the ability to protect the skin from damage by active oxygen.

Ultraviolet rays in sunlight are divided into UVA (long wavelength ultraviolet), UVB (medium wavelength ultraviolet), and UVC (short wavelength ultraviolet), but UVC is blocked by ozone and oxygen and does not reach the surface of the earth. UVA, one of the ultraviolet rays that reach the surface of the earth, penetrates into the dermis and causes stains. On the other hand, UVB causes inflammation on the skin surface and causes pigmentation that causes stains and freckles. It has been clarified that the skin / resident bacteria protect against the damage caused by ultraviolet rays by absorbing ultraviolet rays, which can damage the skin and promote aging.

Example 7 (Ultraviolet absorption action of resident bacteria)

Dilute the cell components of the skin-resident bacterium “Se” to prepare a cell component solution (dilute the 72.3 mgZ ml stock solution 3,000-fold to make it 2.4 g / ml). Clean cream (commercially available summer time clean face; ingredients paraben, cenorole, stearyl alcohol, tocopherol acetate, triethanolamine) , Fragrance, and aloe extract) were similarly diluted (5,400-fold dilution adjustment) and the amount of ultraviolet absorption was measured. The control sunscreen cream produces the same erythema as uncoated at double dose irradiation of SPF12U). The bacterial cell solution and the sunscreen cream solution were each irradiated with ultraviolet rays (Toshiba FL20S / E, distance 25 cm), and the UVA and UVB UV absorption spectra at that time are shown in Fig. 3. Table 8 shows the results. Fig. 3 (a) shows the case of the cell component, Fig. 3 (b) shows the case of the sunscreen clean, and the cell component solution was mixed with the UVA and UVB. It showed an absorption rate of about 90% of the solution. Table 8 Ultraviolet absorption rate (%) of cell component liquid and sans clean

UVA UVB

Cell component liquid 89 94

Sunscreen cream 100 100 In the case of the sunscreen cream, the absorption efficiency decreases over time.However, in the case of indigenous skin-captive bacteria that grow on the skin layer, It constantly grows and protects you from UV damage. It can be said that the growth and activation of skin-resident bacteria according to the present invention have an effect of increasing the ability to protect the skin from ultraviolet damage.

Next, formulation examples of the cosmetic according to the present invention will be described, but the present invention is not limited thereto.

Formulation Example 1 (Lotion)

(% By weight)

Glycerin 3

Polyquinethylene monooleate 1.5

Ethanol 10

Pyrrolidone sodium ruponate Na 2

Fragrance

Keratin 2

Oleic acid 2

Lauric acid 2

Trig receride 3

Lactic acid-sodium lactate 0.5

Purified water balance

100

Prescription example 2 (Claim) (weight%)

Vaseline 4

Evening evening 0.5

Sol Bitan Sesquiole 2

Liquid lanolin 4

Solid paraffin 4

Butyl paraben 0.1

Methylparaben 0.1

Perfume 0.2

Keratin 2

Oleic acid 2

Palmitoleic acid 2

Square 10

Cholesterol ester 0.6

Lactic acid 1

Purified water balance

100

In this formulation, squalene is an unsaturated hydrocarbon and is unstable and difficult to use as a raw material for cosmetics. Example 1 in which squalane with similar biological effects was prescribed

For each of the above formulation examples, 0.2 ml of the base agar medium (03.5 cm dish; yeast extract, cystine hydrochloride, It is excluding Ranio Tray Nsan'na Application Benefits © beam, Application Benefits Puchikaze (BBC) 1.5% hard Toekisu (Nissui) 0.5% glyceryl Li down 1%, 0.2% saline, K ₂ HP0 ₄ 0.2%, promok Resin purple 0.002%, agar 1.5%, pH 6.8, 3ml), apply and absorb, and apply a suspension of normal bacteria suspended from 頰頰唐頰皮皮Cultures were performed, and the growth of colonies of bacteria with normal skin frost was compared and examined by number and size.

Kawaosore indigenous bacteria harvesting, and 20 times stronger abraded with sterile cotton swab soaked in l cm ² sites頰部in saline diluent _{_{(KH 2 P0 4 0.5%,}} Na 2 HP0 4 0.4%: Tsu A method was used in which the bacteria were suspended and suspended in 80 ml of 0.1% in water, 0.03% of cystine hydrochloride, pH 6.8, 2 ml). Tsumugikin floating suspension is 2 serially diluted 10-fold dilution method in the et, each dilution of the liquid 0.05ml was applied on the flat plate, 37 Te, 4 days, anaerobic glove box culture (C0 _2: 10 %, N 2: 80%, H 2: 10%, steel wool method).

In the control lacking the growth factor, hardly any colonies of skin frost indigenous bacteria were observed, and in the control having the growth factor of the skin indigenous bacteria according to the present invention, 10 ^{4 to} 10 ⁶ : f. u / cm ² (c. f. u: colony forming unit) The growth corresponding to the skin area is observed, and the growth factor and the nutrient source according to the present invention are well-balanced. Approximately 1.8 times larger colonies were observed. These colonies were uniform in appearance and all were “Pa” from biochemical characterization (Bergy's Manual of Determinative Bacter iology). In parallel with this, the suspension of diluted skin-resident bacteria collected from the previous skin was transferred to various separation plate media [Triptosoy agar, Mannit salt agar (Nissui Pharmaceutical), potato depot. After aerobic culture (37 ° C, 3-7 days) applied to Kistrose Agar Medium (Nissui Pharmaceutical Co., Ltd.), colonies of bacteria indigenous to captive skin were found in the control lacking growth factors. However, in the case of the present invention having a growth factor of a resident bacterial bacterium, growth corresponding to a skin area of 10 ² to 10 ⁴ cfu Zcm ² was observed. In addition, when the growth factor and the nutrient source according to the present invention are well-balanced, about 1.5 A twice larger colony was observed. These colonies are uniform in appearance

From the biochemical properties test (Bergy's Manual of Determinative Bacteriology), all were “Se”. The above results show the effect of the present test on the growth promotion of growth factors and nutrients of skin-resident bacteria according to the present invention on the growth of skin-resident bacteria "Pa" and "Se". It can be said that.

Test example 2

Actually, after washing the face every morning with a lotion and a cream containing growth factors and nutrients of skin-resident bacteria, and growth-inhibitory factors of skin harmful bacteria, the skin bacteria on the face (頰) are used continuously. The effects on flora and skin were observed. Twenty healthy women (19 to 59 years old) were selected from volunteers, skin bacteria were collected from the same area as in Test Example 1, and skin microbiota was analyzed for 4 weeks. In parallel with this, sebum was collected from the area adjacent to the skin bacteria collection with a cotton swab and analyzed for free fatty acids by gas chromatography. Table 9 shows the results of changes in the number of germs in the skin due to the use of lotion and cream.

Table 9 Changes in the number of skin bacteria with the use of lotion and cream

(Subjects: 20 healthy women, average value from 19 years old to 59 years old) Number of detected colonies (ciu / cm ² ) Fine 前 Before use 2 weeks after use 4 weeks after use Total number of bacteria 5.2X10 ⁵ 8.6X10 ⁵ 9.3X10 ⁵ Bed opening Pionipaku Ϊ Centrum 'Ryo chroma coordinates 5.2X 10 ^{^⁵} 8.6X10 ⁵ 9.3X10 ⁵ Bed [pi Pio two Pakuteriumu Grad [pi -. Sam detection limit below the detection limit below the detection limit or less Sutafi Py »Nokasu' Ebiderumiteisu 0.7X10 ^⁴ ι.οχιο ⁴ 1.8X 10 ⁴ Sutafi ϋ Lactococcus' Kiyabi ί chair ^{^{1. lxlO 3 0.5X10 3 0.1 X 10}} 3 scan evening Fi Π U 'y scum' Ryo Ureusu detection limit below the detection limit below the detection limit or less microphone α Lactococcus detection limit below the detection limit Less than detection limit below Streptococcus-mechas below detection limit below detection limit below detection limit Bacillus below detection limit below detection limit below detection limit below detection limit below detection limit below detection limit

(Note) Below detection limit: <1 xio ² After the start of use, the total number of bacteria showed an increase of about 1.7 times after 2 weeks and about 1.8 times after 4 weeks, which is consistent with the tendency of the most predominant skin It indigenous bacteria `` Pa ''. ing. This skin bacterial count is in the normal range on the skin of healthy subjects and does not indicate abnormal growth. It can be said that this increasing tendency indicates that the proliferation of the resident bacteria "Pa" was promoted.

"Se", the next dominant bacterium, showed a similar increase tendency to "Pa", about 1.4 times after 2 weeks and about 2.6 times after 4 weeks. The number of bacteria of this “Se” is also in the normal range in the skin of healthy subjects and does not indicate abnormal growth. This increasing trend indicates that the growth of skin-resident bacteria “Se” was promoted.

Although a small number of Staphylococcus capitis was detected, the number of phytococcus capitis showed a decreasing trend. In this test, little other skin I bacteria were detected on the selection plates. Table 10 shows changes in the composition of free fatty acids by using lotion and cream.

Table 10 and changes in skin ¾ ^ ¾1§1 »due to use of cream

: ^ «Average value of 20 people) Ratio to fine i»

After two weeks before use creams used 4 weeks total删麵100% 100% 100% Lauric acid _{(C 12: 0) 3 3} 5 myristic acid (C, 4: 0) 9 7 7

No. Palmitic acid _{(C I6: 0) 40 35} 26 palmitic Train acid (ClB: 1) 9 16 18 Stearic acid (C, 8: 0) 20 20 22 Orein acid _{(C 18: 1) 11 12} 14 linoleic赠(C _I8 : 2) 1 1

Linolenic acid ( _C18 : 3)

Arachidic acid (C ₂ : 0) 1 1 1 1 Arachidonic acid (C ₂₀ : 4) 6 5 7 The free fatty acid composition was analyzed by gas chromatography after separating the collected sebum by TLC and methylating it. In the test of this, Lau-phosphate (C _{I 2:} 0) from Araki Don acid: individual differences observed in the total amount of free fatty acids up _{(C 2. 4), 20.8ngZcm} 2 with less human, many people Was 137.8 ng / cm ² . The proportion of various free fatty acids in the total amount of free fatty acids after use, Parumichi phosphate (C _16: 0) indicates a decreasing tendency, Pulse Mi Tray phosphate (C _{1 6: 1)} and the O ray emission Acid ( _C18 : 1) showed an increasing trend. However, these changes are indicators of the lybase activity of the proliferating skin-resident bacteria, and palmitoleic acid inhibits the growth of the skin harmful bacterium “Sa” at low concentrations (Table 3). Also, considering that oleic acid promotes the growth of “Paj and“ Se ”, bacteria indigenous to the skin exist between the bacteria indigenous to the captive skin and the skin in the formation of the“ sebum layer ”. It can be evaluated as a result of being actively involved in maintaining the homeostasis of the ecosystem.

Claims

The scope of the claims

1. A cosmetic composition comprising essential amino acids of skin-resident bacteria as growth factors of skin-resident bacteria and a base.

2. The essential amino acids of the skin-resident bacteria as the growth factors are arginine, isoleucine, leucine, methionine, and phenylalanine.

2. The cosmetic composition according to claim 1, wherein the cosmetic composition is at least one selected from the group consisting of, proline, tritophan, thicin, and parine.

3. A cosmetic composition comprising a higher fatty acid as a growth factor for bacteria resident on the skin and a base.

4. The cosmetic composition according to claim 3, wherein the higher fatty acid as the growth factor is stearic acid, oleic acid or a mixture thereof.

5. A cosmetic composition comprising a higher fatty acid that inhibits the growth of skin harmful bacteria and a base.

6. The higher fatty acids as the growth inhibitory factors are rauric acid, myristic acid, palmitoleic acid, linoleic acid, linolenic acid, arachidic acid and arachidic acid. 6. The cosmetic composition according to claim 5, wherein the cosmetic composition is at least one selected from the group consisting of donic acid.

7. A cosmetic composition comprising a sebum or sweat component and a base as a nutrient source for bacteria resident on the skin.

8. The sebum component as a nutrient source for the skin-resident bacteria was selected from the group consisting of triglycerides, wax esters, squalene, cholesterol esters, and cholesterol. At least one of the components of sweat is at least one selected from the group consisting of water, chlorine, sodium, potassium, calcium, magnesium, nitrogen, ammonia, glucose, and lactic acid 8. The cosmetic composition according to claim 7, wherein the cosmetic composition is a cosmetic composition.

9. A cosmetic composition comprising at least one of the factors or components described in Claims 2, 4, 6, and 8 and a base.