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WO1989000202A3 - Procede de production de nouveaux biopolymeres de polyester - Google Patents

Procede de production de nouveaux biopolymeres de polyester Download PDF

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Publication number
WO1989000202A3
WO1989000202A3 PCT/US1988/002227 US8802227W WO8900202A3 WO 1989000202 A3 WO1989000202 A3 WO 1989000202A3 US 8802227 W US8802227 W US 8802227W WO 8900202 A3 WO8900202 A3 WO 8900202A3
Authority
WO
WIPO (PCT)
Prior art keywords
genes
polymers
phb
biopolymers
physical
Prior art date
Application number
PCT/US1988/002227
Other languages
English (en)
Other versions
WO1989000202A2 (fr
Inventor
Oliver P Peoples
Anthony J Sinskey
Original Assignee
Massachusetts Inst Technology
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Massachusetts Inst Technology filed Critical Massachusetts Inst Technology
Publication of WO1989000202A2 publication Critical patent/WO1989000202A2/fr
Publication of WO1989000202A3 publication Critical patent/WO1989000202A3/fr

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1025—Acyltransferases (2.3)
    • C12N9/1029—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/62—Carboxylic acid esters
    • C12P7/625—Polyesters of hydroxy carboxylic acids

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Polyesters Or Polycarbonates (AREA)

Abstract

Procédé permettant de réguler la synthèse de biopolymères par détermination de la génétique et de l'enzymologie de la biosynthèse de polyhydroxybutyrate (PHB) au stade moléculaire. Les enzymes et gènes purifiés constituent les moyens permettant de mettre au point de nouveaux biopolymères de type PHB ayant des squelettes de polyester. Les objectifs spécifiques sont 1) de commander la longueur de la chaine des polymères produits dans des procédés de fermentation par manipulation génétique, 2) d'introduire différents monomères dans les polymères afin de produire des copolymères ayant différentes propriétés physiques, et 3) d'examiner les propriétés physiques/rhéologiques de ces nouveaux biopolymères afin de mettre au point davantage de critères de conception au stade moléculaire. Le procédé permettant la mise au point de synthèse biopolymère consiste : à isoler et caractériser des gènes pour les enzymes dans la trajectoire synthétique (beta-cetothiolase, réductase d'acétoacétyle CoA et synthétase PHB), à cloner les gènes dans un vecteur(s), à placer le vecteur(s) sous commande d'activateurs régulés, à exprimer les gènes, à déterminer la fonction et l'utilisation d'autres facteurs tels que la spécificité du substrat dans la production et la composition de polymères, et à isoler ainsi qu'à procéder à l'analyse physique et chimique des polymères ainsi obtenus.
PCT/US1988/002227 1987-06-29 1988-06-27 Procede de production de nouveaux biopolymeres de polyester WO1989000202A2 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US6769587A 1987-06-29 1987-06-29
US067,695 1987-06-29

Publications (2)

Publication Number Publication Date
WO1989000202A2 WO1989000202A2 (fr) 1989-01-12
WO1989000202A3 true WO1989000202A3 (fr) 1989-02-09

Family

ID=22077747

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1988/002227 WO1989000202A2 (fr) 1987-06-29 1988-06-27 Procede de production de nouveaux biopolymeres de polyester

Country Status (4)

Country Link
EP (3) EP0688865B1 (fr)
AT (1) ATE212052T1 (fr)
DE (1) DE3856512T2 (fr)
WO (1) WO1989000202A2 (fr)

Families Citing this family (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5245023A (en) 1987-06-29 1993-09-14 Massachusetts Institute Of Technology Method for producing novel polyester biopolymers
US5334520A (en) * 1990-05-25 1994-08-02 Center For Innovative Technology Production of poly-beta-hydroxybutyrate in transformed escherichia coli
ATE172497T1 (de) * 1989-07-10 1998-11-15 Massachusetts Inst Technology Verfahren zur herstellung von polyester- biopolymeren
AU7979691A (en) * 1990-05-25 1991-12-31 Governors Of The University Of Alberta, The Hyperproduction of poly-hydroxyalkanoates during exponential growth by mutant strains of azotobacter vinelandii
GB9115245D0 (en) * 1991-07-16 1991-08-28 Ici Plc Production of polyalkanoate
US5569595A (en) * 1991-09-27 1996-10-29 Center For Innovative Technology Production of poly-β-hydroxybutyrate in prokaryotic host cells
CA2127807A1 (fr) * 1992-11-20 1994-06-09 John Maliyakal Plants de coton transgeniques produisant un bioplastique heterologue
US5811272A (en) * 1996-07-26 1998-09-22 Massachusetts Institute Of Technology Method for controlling molecular weight of polyhydroxyalkanoates
US6143952A (en) * 1998-03-31 2000-11-07 Regents Of The University Of Minnesota Modified pseudomonas oleovorans phaC1 nucleic acids encoding bispecific polyhydroxyalkanoate polymerase
US6103956A (en) * 1998-03-31 2000-08-15 Regents Of The University Of Minnesota Polyhydroxyalkanoate synthesis in plants
AU5247999A (en) * 1998-07-30 2000-02-21 Metabolix, Inc. Production of block copolymers of polyhydroxyalkanoates in biological systems
IT1311922B1 (it) * 1999-04-13 2002-03-20 Nicox Sa Composti farmaceutici.
US20090246430A1 (en) 2008-03-28 2009-10-01 The Coca-Cola Company Bio-based polyethylene terephthalate polymer and method of making same
WO2010101698A2 (fr) 2009-03-03 2010-09-10 The Coca-Cola Company Emballage en poly(téréphtalate d'éthylène) d'origine biologique et son procédé de fabrication
US10072120B2 (en) 2016-12-02 2018-09-11 International Business Machines Corporation Functionalized polyhydroxyalkanoate materials formed from an unsaturated polyhydroxyalkanoate material
US10081706B2 (en) 2017-01-03 2018-09-25 International Business Machines Corporation Side-chain-functionalized polyhydroxyalkanoate materials

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0069497A2 (fr) * 1981-07-07 1983-01-12 Imperial Chemical Industries Plc Copolyesters de l'acide 3-hydroxybutyrique et procédé de leur préparation
EP0202442A2 (fr) * 1985-04-19 1986-11-26 Peter Lázár Dispositif pour commander l'élément chauffant d'un appareil à relier

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3275610A (en) * 1964-03-24 1966-09-27 Mobil Oil Corp Microbial synthesis of polymers

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0069497A2 (fr) * 1981-07-07 1983-01-12 Imperial Chemical Industries Plc Copolyesters de l'acide 3-hydroxybutyrique et procédé de leur préparation
EP0202442A2 (fr) * 1985-04-19 1986-11-26 Peter Lázár Dispositif pour commander l'élément chauffant d'un appareil à relier

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
Chemical Abstracts, vol. 106, no. 5, 02 February 1987, Columbus, OHIO, US, Peoples, Oliver P et al.: "Biosynthetic thiolase from Zooloea ramigera.III. Isolation and characterization of the structural gene", Page 118, Abstract 28324q, & J. Biol. Chem., 1987, 262(1), 97-102 (ENG.) *
European Journal Biochemistry, Vol. 174, 1988, Olivier Ploux et al., "The NADPH-linked acetoacetyl-CoA reductase from Zooloea ramigera", pp 177-182. *
Protein Engineering, 1987, William J. Rutter et al., "Redesigning Proteins via Genetic Engineering", Dale L. Oxender and C. Fred Fox, Alan R. Liss, Inc., New York, pp 257-267. *

Also Published As

Publication number Publication date
EP1229123A3 (fr) 2004-11-03
DE3856512T2 (de) 2002-08-08
EP0688865A1 (fr) 1995-12-27
EP1229123A2 (fr) 2002-08-07
ATE212052T1 (de) 2002-02-15
EP0329770A1 (fr) 1989-08-30
EP0688865B1 (fr) 2002-01-16
WO1989000202A2 (fr) 1989-01-12
DE3856512D1 (de) 2002-02-21

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