[go: up one dir, main page]
More Web Proxy on the site http://driver.im/

US20180024142A1 - Immunoassays for high positively charged proteins - Google Patents

Immunoassays for high positively charged proteins Download PDF

Info

Publication number
US20180024142A1
US20180024142A1 US15/547,080 US201615547080A US2018024142A1 US 20180024142 A1 US20180024142 A1 US 20180024142A1 US 201615547080 A US201615547080 A US 201615547080A US 2018024142 A1 US2018024142 A1 US 2018024142A1
Authority
US
United States
Prior art keywords
synovial fluid
column
sample
protein
human synovial
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
US15/547,080
Other languages
English (en)
Inventor
Elisa Bertotti
Valeria Castagna
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Ares Trading SA
Original Assignee
Ares Trading SA
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Ares Trading SA filed Critical Ares Trading SA
Publication of US20180024142A1 publication Critical patent/US20180024142A1/en
Assigned to ARES TRADING S.A. reassignment ARES TRADING S.A. ASSIGNMENT OF ASSIGNORS INTEREST (SEE DOCUMENT FOR DETAILS). Assignors: CASTAGNA, Valeria, BERTOTTI, Elisa
Abandoned legal-status Critical Current

Links

Images

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/5306—Improving reaction conditions, e.g. reduction of non-specific binding, promotion of specific binding
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C12Q1/40—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving amylase
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54306—Solid-phase reaction mechanisms
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54393—Improving reaction conditions or stability, e.g. by coating or irradiation of surface, by reduction of non-specific binding, by promotion of specific binding
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6887—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids from muscle, cartilage or connective tissue
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/0099—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor comprising robots or similar manipulators
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
    • G01N35/1095—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices for supplying the samples to flow-through analysers
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N2035/00465—Separating and mixing arrangements
    • G01N2035/00495—Centrifuges
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/435—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans
    • G01N2333/475—Assays involving growth factors
    • G01N2333/50—Fibroblast growth factors [FGF]
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2800/00—Detection or diagnosis of diseases
    • G01N2800/10—Musculoskeletal or connective tissue disorders
    • G01N2800/101—Diffuse connective tissue disease, e.g. Sjögren, Wegener's granulomatosis
    • G01N2800/102—Arthritis; Rheumatoid arthritis, i.e. inflammation of peripheral joints

Definitions

  • the capture reagent (sprifermin) was labelled with different ratios of biotin and used to capture the antibody in comparison with the unlabelled antigen.
  • Sprifermin is a highly positively charged protein, therefore a high aspecific binding of the analyte to the column (for example CD column of Gyrolab technology) was observed.
  • the chosen capturing reagent was sprifermin labelled with 1:2 to 1:20 ratio of biotin (such as 1:2, 1:10 or 1:20), preferably 1:5 to 1:15 ratio of biotin or even preferably 1:10 ratio of biotin.

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Food Science & Technology (AREA)
  • Cell Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Robotics (AREA)
  • Peptides Or Proteins (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
US15/547,080 2015-01-29 2016-01-28 Immunoassays for high positively charged proteins Abandoned US20180024142A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP15153117 2015-01-29
EP15153117.5 2015-01-29
PCT/EP2016/051808 WO2016120387A1 (en) 2015-01-29 2016-01-28 Immunoassays for high positively charged proteins

Related Parent Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2016/051808 A-371-Of-International WO2016120387A1 (en) 2015-01-29 2016-01-28 Immunoassays for high positively charged proteins

Related Child Applications (1)

Application Number Title Priority Date Filing Date
US18/164,275 Continuation US20230408529A1 (en) 2015-01-29 2023-02-03 Immunoassays for high positively charged proteins

Publications (1)

Publication Number Publication Date
US20180024142A1 true US20180024142A1 (en) 2018-01-25

Family

ID=52434614

Family Applications (2)

Application Number Title Priority Date Filing Date
US15/547,080 Abandoned US20180024142A1 (en) 2015-01-29 2016-01-28 Immunoassays for high positively charged proteins
US18/164,275 Pending US20230408529A1 (en) 2015-01-29 2023-02-03 Immunoassays for high positively charged proteins

Family Applications After (1)

Application Number Title Priority Date Filing Date
US18/164,275 Pending US20230408529A1 (en) 2015-01-29 2023-02-03 Immunoassays for high positively charged proteins

Country Status (9)

Country Link
US (2) US20180024142A1 (de)
EP (1) EP3250925B1 (de)
JP (1) JP6787904B2 (de)
CN (1) CN107209177A (de)
AU (2) AU2016211950A1 (de)
CA (1) CA2971315C (de)
ES (1) ES2736948T3 (de)
IL (1) IL253518B (de)
WO (1) WO2016120387A1 (de)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US11709155B2 (en) 2017-09-18 2023-07-25 Waters Technologies Corporation Use of vapor deposition coated flow paths for improved chromatography of metal interacting analytes
US11709156B2 (en) 2017-09-18 2023-07-25 Waters Technologies Corporation Use of vapor deposition coated flow paths for improved analytical analysis
JP2020204917A (ja) * 2019-06-17 2020-12-24 株式会社アクロスケール 糖タンパク質等の分析サービス提供クラウドシステム
US11918936B2 (en) 2020-01-17 2024-03-05 Waters Technologies Corporation Performance and dynamic range for oligonucleotide bioanalysis through reduction of non specific binding
WO2024084807A1 (ja) * 2022-10-19 2024-04-25 学校法人東邦大学 線維芽細胞増殖因子１８の測定方法及び測定用試薬

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20120171723A1 (en) * 1996-10-16 2012-07-05 Deisher Theresa A Fgf homologs compositions and uses thereof

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2258788T3 (es) 1996-10-16 2006-09-01 Zymogenetics, Inc. Homologos del factor de crecimiento de fibroblastos.
ATE515515T1 (de) * 2004-03-30 2011-07-15 Glaxo Group Ltd Immunglobuline gegen menschlisches osm
CA2592261C (en) 2004-12-10 2015-11-24 Zymogenetics Inc. Fgf18 production in prokaryotic hosts
UA95461C2 (en) * 2005-09-14 2011-08-10 Арес Трейдинг С.А. Method for quantitative determination of poloxamers
KR101419961B1 (ko) 2006-08-25 2014-07-30 아레스 트레이딩 에스.에이. Ｆｇｆ-18 로 연골 질환의 치료
US7611705B2 (en) * 2007-06-15 2009-11-03 National Cheng Kung University Anti-IL-20 antibody and its use in treating IL-20 associated inflammatory diseases
EP2473637B1 (de) * 2009-09-03 2017-03-29 F. Hoffmann-La Roche AG Verfahren zur behandlung, diagnose und überwachung rheumatoider arthritis
US20140052020A1 (en) * 2012-08-15 2014-02-20 University Of Florida Research Foundation, Inc. Magnetic apparatus and methods of use

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20120171723A1 (en) * 1996-10-16 2012-07-05 Deisher Theresa A Fgf homologs compositions and uses thereof

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
EMD Millipore (2013, retrieved from https://www.google.com/search?source=hp&ei=xS0-XOWLAajJ5gKHxKiYBg&q=an4472en00&btnK=Google+Search&oq=an4472en00&gs_l=psy-ab.3...1618.14305..14757...0.0..0.173.1473.0j10......0....1..gws-wiz.....0..0j0i131j0i10j0i30j0i5i30j0i10i30j0i5i10i30.QhsAJluUhSM) *
Gyrolab Assay Development Guide (2014, retrieved from url://cdn2.hubspot.net/hubfs/378579/3-Gyros/User%20Zone/Gyrolab-assay-developement-guide.pdf). *
Jaydav et al. (Journal of Immunological Methods, 2012;386, pp22-30) *
Roman et. al. (Journal of Pharmacological and Toxicological Methods, 2001; vol. 63, pp227-235) *
Roman Journal of Pharmacological and Toxicological Methods, 2001; vol. 63, pp227-235 *

Also Published As

Publication number Publication date
EP3250925A1 (de) 2017-12-06
EP3250925B1 (de) 2019-04-10
US20230408529A1 (en) 2023-12-21
JP2018509604A (ja) 2018-04-05
CN107209177A (zh) 2017-09-26
ES2736948T3 (es) 2020-01-09
JP6787904B2 (ja) 2020-11-18
IL253518B (en) 2020-06-30
CA2971315A1 (en) 2016-08-04
AU2016211950A1 (en) 2017-07-13
WO2016120387A1 (en) 2016-08-04
AU2021266315A1 (en) 2021-12-09
CA2971315C (en) 2023-02-14
IL253518A0 (en) 2017-09-28

Similar Documents

Publication Publication Date Title
US20230408529A1 (en) Immunoassays for high positively charged proteins
US9759732B2 (en) Assays for detecting the presence or amount of an anti-drug antibody
US9945847B2 (en) Immunoassays using over-labeled fluorescent probes
CN101358969A (zh) 用单一特异性捕获剂定量检测分析物的新方法
US20200132604A1 (en) Methods of determining protein structure using two-photon fluorescence measurements
JP6226976B2 (ja) 多重特異性結合物を検出するための方法
Leary et al. Bioanalytical platform comparison using a generic human IgG PK assay format
US20180128826A1 (en) Method for re-using test probe and reagents in an immunoassay
US11327072B2 (en) Analyte detection immunoassay
WO2005094539A2 (en) Reagents, methods and kits for the universal rapid immuno-detection
Thompson et al. High-throughput quantitation of Fc-containing recombinant proteins in cell culture supernatant by fluorescence polarization spectroscopy
JP4197393B2 (ja) ＩｇＡ腎症の検査法
Dita Enzyme-Linked Immunosorbent Assay (ELISA): A Narrative Literature Review
US20120107844A1 (en) Immunosorbent assay support and method of use
CN107505459A (zh) 定量检测人h‑fabp的时间分辨荧光免疫层析试纸条、试剂盒及其制备方法
CN109781972A (zh) 一种免疫定量检测方法和应用
Zhang et al. Development of a novel capillary electrophoresis-based approach for detection of anti-drug antibody responses
JP6157862B2 (ja) 標的物質−レセプター複合体と遊離レセプターとを分離する方法
EP1346219A1 (de) Analyseverfahren und reagens für die verwendung davon
Banaei et al. PK and PD Ligand-Binding Assays in Large Molecule Drug Development
Kokojka et al. Rapid and specific screening of monoclonal antibodies in hybridoma supernatants by an enzyme-based dipstick immunoassay
TIP ELISA technical guide and protocols
CN115524481A (zh) 通过荧光免疫层析检测技术测定单克隆抗体亲和力的方法
Carroll Development of bead injection methodology for immunoassays

Legal Events

Date Code Title Description
AS Assignment

Owner name: ARES TRADING S.A., SWITZERLAND

Free format text: ASSIGNMENT OF ASSIGNORS INTEREST;ASSIGNORS:BERTOTTI, ELISA;CASTAGNA, VALERIA;SIGNING DATES FROM 20180202 TO 20180222;REEL/FRAME:045071/0658

STPP Information on status: patent application and granting procedure in general

Free format text: NON FINAL ACTION MAILED

STPP Information on status: patent application and granting procedure in general

Free format text: RESPONSE TO NON-FINAL OFFICE ACTION ENTERED AND FORWARDED TO EXAMINER

STPP Information on status: patent application and granting procedure in general

Free format text: FINAL REJECTION MAILED

STPP Information on status: patent application and granting procedure in general

Free format text: DOCKETED NEW CASE - READY FOR EXAMINATION

STPP Information on status: patent application and granting procedure in general

Free format text: NON FINAL ACTION MAILED

STPP Information on status: patent application and granting procedure in general

Free format text: RESPONSE TO NON-FINAL OFFICE ACTION ENTERED AND FORWARDED TO EXAMINER

STPP Information on status: patent application and granting procedure in general

Free format text: FINAL REJECTION MAILED

STPP Information on status: patent application and granting procedure in general

Free format text: DOCKETED NEW CASE - READY FOR EXAMINATION

STPP Information on status: patent application and granting procedure in general

Free format text: NON FINAL ACTION MAILED

STCB Information on status: application discontinuation

Free format text: ABANDONED -- FAILURE TO RESPOND TO AN OFFICE ACTION