US20160199534A1 - Dressing compositions and methods - Google Patents
Dressing compositions and methods Download PDFInfo
- Publication number
- US20160199534A1 US20160199534A1 US14/968,041 US201514968041A US2016199534A1 US 20160199534 A1 US20160199534 A1 US 20160199534A1 US 201514968041 A US201514968041 A US 201514968041A US 2016199534 A1 US2016199534 A1 US 2016199534A1
- Authority
- US
- United States
- Prior art keywords
- cross
- peg
- hydrogel
- dressing
- drug
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Abandoned
Links
Images
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L26/00—Chemical aspects of, or use of materials for, wound dressings or bandages in liquid, gel or powder form
- A61L26/0009—Chemical aspects of, or use of materials for, wound dressings or bandages in liquid, gel or powder form containing macromolecular materials
- A61L26/0052—Mixtures of macromolecular compounds
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L15/00—Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
- A61L15/16—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
- A61L15/22—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons containing macromolecular materials
- A61L15/26—Macromolecular compounds obtained otherwise than by reactions only involving carbon-to-carbon unsaturated bonds; Derivatives thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L15/00—Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
- A61L15/16—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
- A61L15/42—Use of materials characterised by their function or physical properties
- A61L15/44—Medicaments
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L26/00—Chemical aspects of, or use of materials for, wound dressings or bandages in liquid, gel or powder form
- A61L26/0061—Use of materials characterised by their function or physical properties
- A61L26/0066—Medicaments; Biocides
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L26/00—Chemical aspects of, or use of materials for, wound dressings or bandages in liquid, gel or powder form
- A61L26/0061—Use of materials characterised by their function or physical properties
- A61L26/0076—Sprayable compositions
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L26/00—Chemical aspects of, or use of materials for, wound dressings or bandages in liquid, gel or powder form
- A61L26/0061—Use of materials characterised by their function or physical properties
- A61L26/008—Hydrogels or hydrocolloids
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P17/00—Drugs for dermatological disorders
- A61P17/02—Drugs for dermatological disorders for treating wounds, ulcers, burns, scars, keloids, or the like
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/20—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices containing or releasing organic materials
- A61L2300/204—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices containing or releasing organic materials with nitrogen-containing functional groups, e.g. aminoxides, nitriles, guanidines
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/20—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices containing or releasing organic materials
- A61L2300/21—Acids
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/20—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices containing or releasing organic materials
- A61L2300/25—Peptides having up to 20 amino acids in a defined sequence
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/40—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices characterised by a specific therapeutic activity or mode of action
- A61L2300/402—Anaestetics, analgesics, e.g. lidocaine
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/40—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices characterised by a specific therapeutic activity or mode of action
- A61L2300/404—Biocides, antimicrobial agents, antiseptic agents
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/40—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices characterised by a specific therapeutic activity or mode of action
- A61L2300/41—Anti-inflammatory agents, e.g. NSAIDs
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/40—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices characterised by a specific therapeutic activity or mode of action
- A61L2300/412—Tissue-regenerating or healing or proliferative agents
- A61L2300/414—Growth factors
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/60—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices characterised by a special physical form
- A61L2300/602—Type of release, e.g. controlled, sustained, slow
Definitions
- hydrogel-based wound dressings for the promotion of wound healing is accepted clinical practice for wounds that have low to medium exudate. These dressings are typically applied to the skin as an adhesive bandage.
- Hydrogel-based wound dressings are cross-linked polymer gels in sheet form, having a gauze or an impervious polymer backing with an adhesive component provided for skin adhesion. Examples include, hydropolymer dressings impregnated with petroleum gauze or having water-resistant permeable polyurethane backing, paste dressings containing zinc oxide and calamine, waterproof foam dressing made of polyurethane film, gauze-based stretchable dressing, alginate-based dressings, collagen-based dressings and silver dressings.
- Hydrogel sheets are available from several commercial sources, including Tegagel (3M), Vigilon (Bard), Clearsite (Conmed Corporation), AQUASORB (DeRoyal), FLEXDERM (Bertek), NU-GEL (Johnson & Johnson), and CURAGEL (Kendall). These adhesive gauze or patch products, however, remain intact and have the disadvantage of being difficult to remove when peeling off from the skin.
- Hydrogels have also been employed to increase ocular residence time and enhance bioavailability for drugs applied to the eye. The hydrogels were found to provide better tolerability and less blurring of vision than ointments.
- Hydrogels used for ocular application are either pre-formed gels or are formed in situ.
- the pre-formed gels comprise, for example, cellulose derivatives, such as hydroxyethyl cellulose, hydroxymethyl cellulose, carboxymethyl cellulose; polyacrylic acids; cross-linked acrylic acid derivatives (carbomer); polyarcylamides; carbophil; gelatin; hyaluronic acid; polyvinyl alcohol; polyvinyl pyrrolidone; or xanthan gum.
- the in situ-forming gels typically comprise cellulose acetate phthalate, polaxomers, ethylene diamine derivative of polaxamine; psuedolatexes prepared by the incorporation of pilocarpine in cellulose acetate phthalate; various copolymers, such as PEO-PLLA and PEG-PLGA-PEG; cellulose acetophalate latex; Gelrite; carbopol; Matrigel; polyethylene oxide, polyoxypropylene, or gellan gum.
- PEO-PLLA and PEG-PLGA-PEG various copolymers, such as PEO-PLLA and PEG-PLGA-PEG
- cellulose acetophalate latex such as PEO-PLLA and PEG-PLGA-PEG
- Gelrite such as carbopol
- Matrigel polyethylene oxide, polyoxypropylene, or gellan gum.
- polyethylene oxide polyoxypropylene, or gellan gum.
- Thermally gelling polymers (Poloxamer, Pluronics, PEO-PLLA diblock copolymer, PEG-PLGA-PEG triblock copolymer, and Matrigel) have a disadvantage of gelling before administration due to temperature change during packaging or storage, and can require manipulation of the temperature before administration. Furthermore, many of these polymers (e.g., Poloxamer and Pluronics) form a hydrogel which is a viscous, but still flowing solution and therefore are not readily applicable for use at a particular site on body surfaces.
- a hydrogel which can form in situ and is readily soluble, i.e., has reversible cross-linkage, can provide certain advantages as a wound dressing or drug delivery device at the site of application. These advantages include being relatively easy and painless to remove from the site of application, as well as being highly conformable to the site of application, such as a wound, the eye, or surrounding tissue.
- an in situ-forming hydrogel that can be administered as a spray can provide an advantageous method of application to the site.
- a spray-on gel for the topical delivery of drug to the eye, and having the drug or drugs linked to RGD peptides, which exhibit adhesive properties, can enhance the ocular residence time for the linked drugs.
- the subject invention concerns compositions, drug delivery devices and methods relating to in situ-forming hydrogels useful to form a protective covering over a wound or damaged area of the skin, such as cut or abrasion, a surgical site, or a blistered surface resulting from disease or trauma, such as a burn.
- An in situ-forming hydrogel composition of the subject invention can also be useful as a carrier for a drug or drugs administered by topical or transdermal application, including ocular application.
- the hydrogels of the subject invention can be adherent, i.e., the composition, itself, can have adherent or adhesive properties.
- the subject invention concerns a wound or ocular dressing comprising a first component comprising a hydrophilic polymer having a sulfhydryl, thiol, or mercaptan moiety; and a second component comprising a cross-linker, said cross-linker forming reversible cross-linkages with the hydrophilic polymer.
- the first and second components form a material that adheres to skin of a mammal and acts as a wound dressing.
- the polymer is polyethylene glycol, and more preferably, the polyethylene glycol comprises a sulfhydryl, thiol, or mercaptan moiety to form disulfide bonds.
- the composition can also include a peptide, such as RGD peptide, the RGD peptide preferably being derivatized to the polyethylene glycol component.
- the composition can alternatively or additionally include a drug or combination of drugs or a growth factor.
- the subject invention also concerns a method of treatment for delivering a drug to a corneal surface of an eye. This method comprises the steps of:
- Removal of the hydrogel dressing preferably comprises reversing the cross-links using a reducing agent wherein the reducing agent is preferably cysteine or derivatives thereof, cysteine ethyl ester, cysteine methyl ester, gluthatione, cysteine hydrocholoride, dithiothretol, N-Ethylmalemide, phosphine derivatives tetrakis-hydroxymethyl phosphonium chloride and tris-diethylaminomethyl phosphine trialkylphosphine agents, such as Tris[2-carboxyethyl] phosphine and mercaptoethanols, 2,3-dimercapto-1-propanol, dinitrobenzoic acid, a thiol, a mercaptan, a sulfite or bisulfite or ammonium or sodium salts thereof, thioglycolic acid, thiolactic acid, cysteine, thioglycerol, thio
- the subject invention further includes a method of preparing a cross-linked hydrogel composition for application to the skin, said method comprising:
- a preferred embodiment of a composition of the subject invention comprises novel cross-linkers, such as RGD-derivatized PEG, and further can comprise RGD-linked drug.
- a hydrogel composition of the subject invention can comprise additional components or ingredients, including polyvinylpyrrolidone (PVP), propylene glycol, low molecular weight PEG ( ⁇ 6000 Da), glycerin, or cellulose derivatives such as hydroxypropyl cellulose, hydroxylpropyl methylcellulose, methylcellulose, or the like, as necessary to provide desired properties for the hydrogel in accordance with the functionalities as recognized in the art.
- PVP polyvinylpyrrolidone
- propylene glycol propylene glycol
- low molecular weight PEG ( ⁇ 6000 Da) low molecular weight PEG ( ⁇ 6000 Da)
- glycerin glycerin
- cellulose derivatives such as hydroxypropyl cellulose, hydroxylpropyl methylcellulose, methylcellulose, or the like
- the polymeric PEG component of the subject composition preferably comprises a sulfhydryl, thiol, or mercaptan moiety capable of forming a reversible disulfide bond or bridge for cross-linkage of the polymer.
- a preferred composition of the hydrogel according to the subject invention comprises a thiol-terminated PEG and several substances that are useful for cross-linking the thiol groups. Alternately, a PEG having a maleimide, thiopyridine or vinylsulfone termination can be used. At physiological pH and temperatures, cross-linking of PEG into a hydrogel can occur in about 1-3 minutes.
- PEG offers several other advantages, including its chemoselective properties, its capability to form reversible and non-reversible cross-links, its free thiol group for covalently linking to drug, its property of blocking proteolytic enzymes and immune system components that can cause an inflammatory reaction, and its commercial availability in numerous forms.
- the sulfhydryl or thiol or mercaptan-terminated PEG can reversibly bond to a sulfur-terminated moiety of a peptide, such as a peptide comprising the amino acid chain Arg-Gly-Asp (RGD) or a sulfur-containing amino acid, such as Cys.
- a peptide such as a peptide comprising the amino acid chain Arg-Gly-Asp (RGD) or a sulfur-containing amino acid, such as Cys.
- RGD Arg-Gly-Asp
- Cys sulfur-containing amino acid
- one preferred peptide used in accordance with the subject invention comprises Cys attached to the Asp amino acid of the RGD peptide.
- Use of a PEG-based polymer allows the hydrogel to be functionalized using these peptides to enhance the wound healing and the adhesive properties of the gel.
- Peptides typically used for this purpose include those having the sequence ‘Arg-Gly-Asp,’ or RGD, in cyclic or linear form.
- the heretofore undisclosed PEG polymers derivatized to include RGD peptide useful as cross-linker in a spray-on gel can advantageously provide bioadhesive and wound-healing properties to the formed hydrogel.
- the PEG and cross-linker can be provided as separate solutions, being mixed during administration to the site, for example, provided as streams of solutions from separate sources or containers and administered simultaneously, allowing the gel and cross-linker solutions to mix together during administration, so that the cross-linked gel matrix sets in less than 30 minutes, and preferably less than 10 minutes.
- the disulfide bonds of the resultant cross-linked hydrogel matrix can provide the support to maintain the integrity of the gel, as well as the capability to adsorb into the gel the exudate from the wound or other site.
- the cross-linking within the polymeric composition can be readily reversed using a reducing agent.
- Such spray-on hydrogels having reversible cross-links for advantageous application to skin have not been previously disclosed.
- a preferred object of the invention is to provide enhanced patient compliance for a wound dressing by applying a hydrogel having reversible cross-links, e.g., cross-links containing disulfide bridges, so that the hydrogel wound dressing can be readily washed off by dissolution of the hydrogel rather than physical removal, such as peeling off, of the intact dressing.
- a hydrogel having reversible cross-links e.g., cross-links containing disulfide bridges
- the drugs and other active components used in accordance with the subject invention can be dissolved or dispersed in the cross-linked polymeric matrix. Drugs which can provide anesthetic, antimicrobial, or wound healing properties are preferred for use in an embodiment directed to a protective covering for a wound. Alternatively, the drugs can be linked to an RGD peptide derivatized onto the PEG polymer.
- hydrogel cross-linked with RGD derivatized PEG cross-linker
- RGD derivatized PEG cross-linker can provide dermal retentive properties when applied to the skin, thereby providing prolonged release properties for the drug released from the hydrogel.
- This embodiment of a hydrogel of the subject invention can also enhance the cell adhesion of the drugs onto a corneal surface.
- the subject invention preferably comprises a hydrogel which is formulated to be applied or administered, preferably as a liquid and more preferably as a spray, wherein the formed gel comprises a reversibly cross-linked polymeric matrix or network.
- the cross-linking component is also preferably formulated as a liquid and more preferably as a spray.
- the hydrogel and cross-linking components are therefore preferably applied concomitantly as separate liquids and more preferably administered as a spray wherein the two liquids are mixed during the application thereof.
- the hydrogel is soluble, and can be dissolved and easily removed from the site rather than requiring the gel to be removed intact, e.g., peeled, from the skin.
- the spray formulation for the gel of the subject invention comprises cross-linked, water-soluble polyethylene glycol (PEG) polymers.
- PEG is advantageously a hydrogel-forming component that is well known for its safe and non-toxic properties.
- It is another object of the present invention to provide a spray-on hydrogel system comprising a dual-source nozzle, such as a dual barrel syringe or a pressurized spray can, capable of concomitant spraying of a stream of polymeric solution and a stream of cross-linker solution.
- This spray-on hydrogel system can provide advantageous topical delivery to the eye or skin of an in situ-forming hydrogel containing a drug or drugs.
- a further object of the invention is to provide a controlled-release drug delivery system comprising a hydrogel wherein the drug or drugs are delivered from or through the hydrogel composition for a sustained or extended period of time.
- the drug or drugs can be linked to RGD peptides incorporated into a PEG-based hydrogel. Linkage of drug to the RGD peptide component of the hydrogel can increase residence time in the ocular region or on the skin cells to increase their residence time at the site of application, thereby providing relatively high local concentrations and prolonged release and action of the drug or drugs.
- kits for applying a wound or ocular dressing or a hydrogel capable of delivering drug to the site of application can comprise a polymer-forming composition and a reversible cross-linking composition, wherein the polymer-forming and cross-linking compositions can be mixed to form a reversible, cross-linked hydrogel which rapidly gels to form a wound dressing or drug delivery device at the site of application.
- the kit can also include a composition containing drug, growth factor or other wound-healing enhancer, either separate from the polymer-forming composition and the cross-linking composition, or drug can be incorporated into either of these compositions.
- the kit can comprise a separately contained reducing agent to reverse the cross-linkage of the formed hydrogel, thereby providing a means for dissolving the hydrogel for its easy removal from the site without having to remove the hydrogel intact.
- FIG. 1 depicts formation of disufide bridges (cross-links) leading to hydrogel based on 8-armPEG-SH and 8-armPEG-S-TP (thiopyridine).
- FIG. 2 depicts formation of disufide bridges (cross-links) leading to hydrogel based on 4-armPEG-SH and 4-armPEG-S-TP (thiopyridine).
- FIG. 3 depicts formation of disufide bridges (cross-links) leading to hydrogel based on 8-armPEG-SH and 4-armPEG-S-TP (thiopyridine).
- FIG. 4 depicts formation of disufide bridges (cross-links) leading to hydrogel based on 4-armPEG-SH and 8-armPEG-S-TP (thiopyridine).
- FIG. 5 depicts formation of disufide bridges (cross-links) leading to hydrogel based on 4-armPEG-SH and H 2 O 2
- FIG. 6 depicts formation of disufide bridges (cross-links) leading to hydrogel based on 8-armPEG-SH and H 2 O 2 .
- FIG. 7 depicts formation of thioether bonds (cross-links) leading to hydrogel based on 8-armPEG-SH and BMPEO 3 .
- FIG. 8 depicts formation of thioether bonds (cross-links) leading to hydrogel based on 8-armPEG-SH and BMPEO 2 .
- FIG. 9 depicts formation of hydrogel from a dual barrel syringe containing a polymer component and a cross-lining component in the respective barrels.
- FIG. 10 depicts removal of the gel using the reducing agent solution to cleave the disulfide bonds.
- FIG. 11 shows the DSC results for the melting of the 8-arm PEG-S-TP product.
- FIG. 12 depicts attaching the RGD peptide on the 8-arm-Peg-SH.
- FIG. 13 shows the DSC results for the melting of the RGD attached at 5 sites of 8-arm PEG-S-TP.
- FIG. 14 shows the DSC results for the melting of the RGD attached at 2 sites of 8-arm PEG-S-TP.
- FIGS. 15A and 15B shows the Rheology of hydrogels of 8-arm Peg-SH and H 2 O 2 in buffer (A) and Rheology of hydrogel with additives (B).
- FIGS. 16A and 16B shows the Rheology of hydrogels of 8-arm Peg-SH and 8-arm Peg-S-TP in buffer (A) and Rheology of hydrogel with additives (B).
- FIG. 17 shows the ESI-MS spectrum of the Olvanil-Cysteine product.
- FIG. 18 shows the MALDI-TOF spectrum of the PEG-Olvanil-Cysteine product.
- the subject invention concerns a composition
- a composition comprising a drug or other active ingredient, a polymeric hydrogel-forming component and a cross-linker, providing in situ formation of the hydrogel when sprayed topically onto an area of the body.
- the subject hydrogel composition advantageously has bioadhesive properties and reversible cross-links.
- a preferred embodiment of the subject composition comprises a poly(ethylene glycol), or PEG, hydrogel that, when applied topically, provides an adhesive gel which adheres well to a body surface such as the cornea or the skin. Further, the subject composition can provide modulated drug release from the hydrogel so that drug can be released over a prolonged period of time, e.g., several hours or days, or up to about a week.
- the hydrogel composition of the subject invention preferably comprises RGD peptide-derivatized components, including (a) RGD peptide linked to the PEG to provide cell adhesive and wound-healing properties for the composition, or (b) RGD peptide linked to a drug or drugs used in the compositions to provide increased retention of drug at site of application and for prolonged release of drug to promote or enhance wound healing.
- RGD peptide it is meant a peptide comprising RGD.
- the polymeric hydrogel and cross-linker are formulated as separate solutions and concomitantly introduced, e.g., sprayed, onto the target site such that the polymer and cross-linker solutions adequately mix to form in situ a cross-linked hydrogel network or matrix.
- Active drug or drugs can be incorporated into one or both of the solutions, and are preferably linked to the hydrogel forming polymer through an RGD peptide linkage wherein the RGD peptide is incorporated or derivatized into the polymer.
- the cross-linked hydrogel so formed can provide a protective barrier on an injured or affected area, thereby serving as a wound dressing, or can provide a composition for topical drug delivery.
- the hydrogel base comprises PEG (polyethylene glycol) or PEG derivatives.
- PEG polyethylene glycol
- a hydrogel formed from PEG is advantageously flexible, elastic and strong, enabling attachment on the eye, skin and or injured parts thereof.
- certain derivatized PEGs such as PEGs derivatized with an RGD peptide, can enhance wound healing, anchor onto the injured site and deliver the drugs for extended periods of time ranging from hours, to days, or up to about a week.
- a PEG-based hydrogel is highly permeable, allowing diffusion of incorporated drugs, salts, water and gases. Satisfactory gel formation can be achieved using a ratio of polymer to cross-linker from about 5:1 to about 1:5.
- the concentration of PEG can be varied from about 2% to about 30% (w/w) in the hydrogel, and in certain embodiments is preferably about 8% to about 10%.
- the polymer and cross-linker are preferably dissolved in buffer in the pH range 4-9 and preferably in the pH range 5-8 to obtain the gels.
- the hydrogel composition such as PEG-based composition
- the drugs can be either physically entrapped or modified drugs with cleavable bonds can be physically incorporated or covalently linked into the hydrogel to provide controlled release, which is otherwise not possible for highly hydrophilic drugs which traverse easily through the gel.
- a composition according to the present invention can also be applied to skin for burns associated with fire, sunburn and chemical irritants, as well as physical injuries such as bed sores by providing a scaffold for seeding and repair of the damaged skin.
- a distinctive feature of the hydrogel of the subject invention is the formulation of reversible cross-linking such that the hydrogel matrix can be dissolved and readily washed off. This feature can advantageously minimize disruption of newly formed skin when removing the reversibly cross-linked hydrogel dressing, offering advantages over the typical gel bandages, which are required to be physically removed intact, e.g., by peeling off of the dressing, which can cause discomfort or further trauma to the wound site.
- the hydrogel composition can further comprise one or more drugs for delivering drug for treatment at the site.
- the drug or drugs can be physically entrapped within the matrix of the formed hydrogel or can be covalently linked to hydrogel, such as by the RGD peptide.
- Such drug or drugs can be wound healing enhancers, such as RGD peptides, antiseptics or antibiotics, anti-inflammatories, anesthetics, pain relievers, or drugs useful for in situ treatment, such as drugs for treating glaucoma at an ocular site, and growth factors.
- These drugs include, but are not limited to lidocaine, benzocaine, butamben, dibucaine, oxybuprocaine, pramoxine, proparacaine, proxymetacaine, novocaine, procaine, tetracaine, doxycycline, minocycline, oxytetracycline, sancycline, dedimethylamino tetracycline, indomethacin, diclofenac, ibuprofen, naproxen, ketoprofen, dexamethasone, a vallinoid, olvanil, capsaicin, benzalkonium chloride, an antiglaucoma medication, pilocarpine, timolol, levobunolol, betaxolol, or carbacol.
- the invention is not limited to the use of the drugs mentioned above and can be extended to other therapeutic agents which aid in wound healing processes when used for topical skin delivery or drugs used in ocular treatments
- a hydrogel of the subject invention can include a growth factor for promoting wound healing.
- the growth factors useful in accordance with the subject invention include the cytokines such as epidermal growth factor (EGF), including all members of the EGF family of proteins having one or more repeats of the conserved amino acid sequence: CX7CX4-5CX10-13CXCX8GXRC (where X represents any amino acid), transforming growth factor alpha (TGF-alpha), Transforming Growth Factor beta (TGF-b), keratinocyte growth factor (KGF-2), fibroblast growth factor fibronectin, fibrinogen, Granulocyte-Monocyte Colony Stimulating Factor (GM-CSF) and platelet-derived growth factor (PDGF).
- EGF epidermal growth factor
- TGF-alpha transforming growth factor alpha
- TGF-b Transforming Growth Factor beta
- KGF-2 keratinocyte growth factor
- GM-CSF Granulocyte-Monocyte Colony Stimulating Factor
- the subject invention preferably comprises a hydrogel which is formulated to be applied or administered, preferably as a liquid and more preferably as a spray, wherein the formed hydrogel comprises a reversibly cross-linked polymeric matrix or network.
- the hydrogel is soluble, and can be dissolved and easily removed from the site rather than requiring the gel to be removed intact, e.g., peeled, from the skin.
- the cross-linking within the polymeric composition can be readily reversed using a reducing agent.
- substances that have the ability to reduce other substances are said to be reductive and are known as reducing agents, reductants, or reducers.
- reducing agents can transfer electrons to another substance, and is thereby, itself, oxidized. Because reducing agents “donate” electrons, they are also called electron donors.
- Thiol-disulfide exchange is the principal reaction by which disulfide bonds are formed and rearranged. Disulfide reshuffling is a faster reaction.
- Thiol-disulfide exchange is a chemical reaction in which a thiolate group S— attacks a sulfur atom of a disulfide bond —S—S—. The original disulfide bond is broken, and its other sulfur atom is released as a new thiolate, carrying away the negative charge. Meanwhile, a new disulfide bond forms between the attacking thiolate and the original sulfur atom.
- the transition state of the reaction is a linear arrangement of the three sulfur atoms, in which the charge of the attacking thiolate is shared equally.
- the protonated thiol form —SH is unreactive, i.e., thiols cannot attack disulfide bonds, only thiolates.
- the thiolate of a redox reagent such as glutathione or dithiothreitol attacks the disulfide bond.
- the several reducing agents that either block or reverse the disulfide bridges forming the cross-linkage in accordance with the subject invention include cysteine, cysteine ethyl ester, and cysteine methyl ester, gluthatione, cysteine hydrocholoride, dithiothretol, N-Ethylmalemide, phosphine derivatives tetrakis-hydroxymethyl phosphonium chloride and tris-diethylaminomethyl phosphine trialkylphosphine agents, such as Tris[2-carboxyethyl] phosphine and mercaptoethanols, 2,3-dimercapto-1-propanol, and dinitrobenzoic acid.
- cysteine cysteine ethyl ester
- cysteine methyl ester gluthatione
- cysteine hydrocholoride dithiothretol
- N-Ethylmalemide phosphine derivatives tetrakis-hydroxy
- reducing agents appropriate for use in accordance with the subject invention are not limited to these and can be any compound having thiols or mercaptan groups as well as sulfites and/or bisulfites.
- Mercaptans and thiols which can be used to reverse the disulfide linkages in a reversibly cross-linked hydrogel of the subject invention include thioglycolic acid, thiolactic acid, cysteine, thioglycerol, thioglycolic hydrazide, thioglycolamide, glycerol monothioglycolate, beta-mercapto-propionic acid, N-hydroxyethyl mercapto-acetamide, N-methyl mercapto-acetamide, beta-mercapto-ethylamine, beta-mercapto-propionamide, 2-mercapto-ethanesulfonic acid, dimercapto-adipic acid, dithiothreitol, homocysteine
- Preferred reducing agents of the present invention are cysteine or cysteine derivatives and glutathione.
- Various concentrations of these agents will be effective in degrading the hydrogel matrix. The higher the concentration, the faster the degradation. However, the lowest effective concentration is preferably used in order to minimize adverse tissue reactions.
- a hydrogel composition of the present invention is further suitable for application as an ocular gel.
- a hydrogel composition of the subject invention can comprise an RGD-containing peptide having bioadhesive properties, linked to the polymeric (e.g., PEG) component for enhancing the adhesion to the cells.
- RGD-linked drugs incorporated in the gels provide prolonged ocular residence times, enhancing the ocular bioavailability of the drugs.
- compositions, components thereof, and properties of embodiments of the subject invention are provided hereinbelow.
- the hydrogel network or matrix composition is obtained by formation of disulfide bridges or formation of thioether bonds in the PEG (polyethylene glycol) having a thiol group (reduced sulfhydryls groups ‘SH’) at each terminus.
- PEG polyethylene glycol
- SH reduced sulfhydryls groups
- BMPEO 2 bis-maleimido diethylene glycol
- BMPEO 3 bis-maleimido triethylene glycol
- BMOE Bis-Maleimidoethane
- BMH bismaleimidohexane
- the PEG polymers having thiol terminus groups include 2-, 3-, 4-, 8-arm or multiple arm thiol PEGs in the molecular weight range of 2,000 to 100,000 Da.
- the hydrogels can be obtained by cross-linking the PEG having a maleimide or thiopyridine terminated groups with compounds having thiol functionality.
- One of the embodiments of the present invention includes the gels based on thiol terminated PEGs (e.g., 8-arm-PEG-SH) cross-linked with H 2 O 2 .
- the in situ forming hydrogel was obtained by spraying the solution of the thiol terminated PEG and the cross linker: H 2 O 2 , in phosphate buffer pH 8 from a dual barrel syringe to obtain the hydrogel.
- Formulations according to the subject invention having various concentrations of hydrogel component, mixed with varying volumes of 3% H 2 O 2 as a cross-linker, and their relatively rapid gelling time, are shown in Tables 1-3.
- Another embodiment of the present invention includes hydrogels of thiol terminated PEGs (e.g. 8-arm-PEG-SH) cross-linked with maleimide cross-linkers.
- the in situ forming hydrogel was obtained by mixing the solutions of the thiol terminated PEG and the cross-linker having a maleimide termination BMPEO 3 (bis-maleimido triethylene glycol) in phosphate buffer pH 5.38 and 7.4 to obtain the hydrogels.
- BMPEO 3 bis-maleimido triethylene glycol
- Yet another embodiment of the present invention is a gel based on the 4-arm-PEG-SH and 8-arm-PEG-SH cross-linked with 4-arm and 8-arm-PEG respectively having thiopyridine terminal groups.
- the thiol terminated PEG (4 and 8-arm PEG-SH) is treated with three fold excess of dithiodipyridine (aldrithiol) in alcohol under mild acidic conditions overnight at room temperature.
- the product, a thiopyridine terminated PEG (4 arm PEG-S-TP and 8-arm PEG-S-TP) so obtained is purified using the size exclusion chromatography. This PEG-S-TP product is used for the gel formation.
- the in situ forming hydrogel was obtained by spraying the solution of the thiol terminated PEG and the cross-linker: having a thiopyridine termination developed in-house, from a dual barrel syringe to obtain the hydrogel.
- the solution of polymer and cross-linker were made in phosphate buffer pH 8.
- the thiopyridine terminated cross-linker was obtained from the 4- and 8-arm thiol terminated PEG, respectively, as also shown in Table 5.
- the concentration of the polymer solution and the cross-linker was varied to yield the hydrogel almost instantaneously, as shown in Table 6.
- the hydrogel is obtained by the formation of the disulfide bridges either inter or intramolecular in the thiol terminated PEG.
- one of the embodiments of the present invention discloses the use of the peptide sequence, Arg-Gly-Asp (RGD), which is naturally present in many proteins involved in adhesion of cells to other cells and to basement membrane. As a result of better contact, this provides better transfer of a drug from the gel to the site of application. Furthermore, the presence of RGD sequences can be recognized by cellular receptors, thereby serving as attachment sites on the corneal epithelial cells or keratinocytes cells on the skin. The RGD is known to accelerate skin and wound repair.
- RGD peptide comprising the ‘Arg-Gly-Asp’ sequence, such as the linear peptide Arg-Gly-Asp-Cys, Gly-Arg-Gly-Asp-Ser, Gly-Arg-Gly-Asp-Ser-Pro, or as the cyclic peptide Cyclo-Arg-Gly-Asp-Try-Lys, were used to enhance the adhesion of the gel on the cells and were synthesized as described below.
- the hydrogel was obtained by spraying the solution of thiol terminated PEG (8-armPEG-SH) and the cross-linker consisting of PEG having partial thiopyridine and RGD terminal groups (RGDC-8-armPEG-S-TP), in phosphate buffer pH 8.
- the hydrogel composition is shown in Table 7.
- a spray-on hydrogel was obtained by spraying the solution of thiol terminated PEG (8-arm PEG-SH) and along with the cross-linker solution consisting of (a) thiopyridine terminated PEG (8-arm PEG-S-TP) and (b) PEG having partial thiopyridine and RGD terminal groups (RGDC-8-armPEG-S-TP).
- the solution of polymer and cross-linker were mixed in phosphate buffer pH 8.
- the hydrogel results instantaneously.
- the hydrogel compositions are shown in Table 8.
- a spray-on hydrogel was obtained by dissolving the thiol terminated PEG (4-arm-PEG-SH and 8-arm PEG-SH) and the thiopyridine terminated PEG (4-arm-PEG-S-TP and 8-arm PEG-S-TP) (Formulation in accordance with Example 1c), and an additive comprising a solution of polyvinyl pyrrolidone (PVP) in phosphate buffer pH 8.
- PVP polyvinyl pyrrolidone
- the concentration of PVP was varied from 1.5-2% w/v as shown in Table 9.
- Two spray-on hydrogels were obtained by dissolving the thiol terminated PEG (8-arm PEG-SH) and the thiopyridine terminated PEG (8-arm PEG-S-TP) (in accordance with a formulation of Example 1c) with additives comprising (a) a solution of 2% w/v polyvinylpyrrolidone (PVP) and 5% v/v of glycerin in phosphate buffer pH 8, and (b) by solution of 2% w/v polyvinyl pyrrolidone (PVP), 5% v/v of glycerin and 5% v/v of polyethylene glycol (MW 600) in phosphate buffer pH 8.
- additives comprising (a) a solution of 2% w/v polyvinylpyrrolidone (PVP) and 5% v/v of glycerin in phosphate buffer pH 8
- PVP polyvinylpyrrolidone
- PVP polyvinyl pyrroli
- a spray-on hydrogel was obtained by dissolving the thiol terminated PEG (4-arm-PEG-SH and 8-arm PEG-SH) and the thiopyridine terminated PEG (4-arm-PEG-S-TP and 8-arm PEG-S-TP) in a solution of polyvinylpyrrolidone (PVP) and hydroxypropyl methylcellulose (HPMC) in phosphate buffer pH 8.
- PVP polyvinylpyrrolidone
- HPMC hydroxypropyl methylcellulose
- the drugs Lidocaine and Doxycycline Hyclate were incorporated in the hydrogel by dissolving the drugs in the polymer solution (8-arm PEG-SH) as shown in Table 12.
- the drug incorporated polymer solution was cross-linked using the thiopyridine terminated PEG (8-arm PEG-S-TP) in phosphate buffer pH 8.
- the drug incorporated polymer solution was cross-linked using the thiopyridine terminated PEG (8-arm PEG-S-TP) in a solution of 2% w/v polyvinyl pyrrolidone (PVP) and 5% v/v of glycerin in phosphate buffer pH 8 as shown in Table 12.
- PVP polyvinyl pyrrolidone
- the drug Doxycycline Hyclate was incorporated in the hydrogel by dissolving the drug in the polymer (8-arm-PEG-SH) solution in phosphate buffer pH 8.
- the drug incorporated polymer solution was cross-linked using the H 2 O 2 solution as shown in Table 13.
- the drug indomethacin was incorporated in the hydrogel by dissolving the drug in the polymer solution (8-arm PEG-SH) as shown in Table 14.
- the drug incorporated polymer solution was cross-linked using the thiopyridine terminated PEG (8-arm PEG-S-TP) in phosphate buffer pH 8.
- the drug incorporated polymer solution was cross-linked using the thiopyridine terminated PEG (8-arm PEG-S-TP) in a solution of 2% w/v polyvinylpyrrolidone (PVP) and 5% v/v of glycerin in phosphate buffer pH 8 as shown in Table 14.
- PVP polyvinylpyrrolidone
- Doxycycline-RGD-PEG was incorporated in the hydrogel by dissolving the same in the polymer solution (8-arm PEG-SH) as shown in Table 15.
- the drug incorporated polymer solution was cross-linked using the thiopyridine terminated PEG (8-arm PEG-S-TP) in phosphate buffer pH 8.
- the drug incorporated polymer solution was cross-linked using the thiopyridine terminated PEG (8-arm PEG-S-TP) in a solution of 2% w/v polyvinylpyrrolidone (PVP) and 5% v/v of glycerin in phosphate buffer pH 8 as shown in Table 15.
- the Doxycycline-RGD-PEG component was synthesized and the synthetic procedure is given below.
- PEG 10-20 KDa
- PEG 10-20 KDa
- thiol termination 2,4 and 8-arm Peg-SH
- the amount of Doxycycline-RGD can be taken proportional to the number of arms/sites at which it is required to be appended on the 4 or 8-arm PEG-SH.
- PEG 10-20 KDa
- PEG-RGD 2,4 and 8-arm Peg-SH
- the amount of indomethacin-PEG-RGD can be taken proportional to the number of arms/sites at which it is required to be appended on the 4 or 8-arm PEG-SH.
- the drug olvanil was be incorporated in the hydrogel by dispersing the drug in the polymer solution (8-arm PEG-SH).
- the drug incorporated polymer solution was cross-linked using the thiopyridine terminated PEG (8-arm PEG-S-TP) in phosphate buffer pH 8.
- the drug incorporated polymer solution was cross-linked using the thiopyridine terminated PEG (8-arm PEG-S-TP), both the polymer and cross-linker were dissolved in a solution of 2% w/v polyvinylpyrrolidone (PVP) and 5% v/v of glycerin in phosphate buffer pH 8 as shown in Table 15.
- PVP polyvinylpyrrolidone
- the PEG-Olvanil-Cysteine was incorporated in the hydrogel by dissolving the same in the polymer solution (8-arm PEG-SH).
- the drug incorporated polymer solution was cross-linked using the thiopyridine terminated PEG (8-arm PEG-S-TP) in phosphate buffer pH 8.
- the drug incorporated polymer solution was cross-linked using the thiopyridine terminated PEG (8-arm PEG-S-TP) in a solution of 2% w/v polyvinylpyrrolidone (PVP) and 5% v/v of glycerin in phosphate buffer pH 8.
- PVP polyvinylpyrrolidone
- the PEG-Olvanil-Cysteine component was synthesized and the synthesis procedure is given below.
- Step 1 The 3-fold excess of Fmoc-Cysteine(S-Trt)-COOH was reacted with Olvanil in presence of diisopropylcarbodiimide under basic conditions by adding pyridine in dimethyl formamide. The formation of the Olvanil-Cysteine product was analyzed using ESI-MS as shown in FIG. 17 .
- Step 2 Olvanil-Cys(trt) ester was linked to 5 kDa-PEG-NHS in the presence of N N-Diisopropylethylamine by dissolving in dimethyl formamide by stirring overnight.
- the product so obtained was purified by Size exclusion chromatography using G-25 Sephadex beads. The formation of the product was analyzed using MALDI-TOF as shown in FIG. 18 .
- One of the embodiments of the present invention includes a therapeutic agent or drug, such as lidocaine (a topical anesthetic), benzalkonium chloride (a topical antiseptic), olvanil (an anti-inflammatory agent), doxycycline (an antibiotic), pilocarpine or protease inhibitors incorporated into the hydrogel composition.
- a therapeutic agent or drug such as lidocaine (a topical anesthetic), benzalkonium chloride (a topical antiseptic), olvanil (an anti-inflammatory agent), doxycycline (an antibiotic), pilocarpine or protease inhibitors incorporated into the hydrogel composition.
- Formulations incorporating the drugs into the hydrogel composition are shown in Tables 12-15.
- drugs linked to RGD peptide appended onto PEG were incorporated in the hydrogel composition, e.g., doxycycline as disclosed in Example 5.
- D. The RGD peptide, comprising the ‘Arg-Gly-Asp’ sequence, such as the liner peptide or the cyclic peptides are disclosed.
- linear peptide examples include Arg-Gly-Asp-Cys, Gly-Arg-Gly-Asp-Ser, Gly-Arg-Gly-Asp-Ser-Pro, and the cyclic peptide include, Cyclo-Arg-Gly-Asp-Try-Lys but are not so limited, and can be extended to any peptide having the sequence ‘Arg-Gly-Asp’.
- prodrugs are designed to provide cell adhesive and retentive properties to enhance binding to corneal epithelial cells or to the extracellular matrix of the injured skin for slow-release the active drug.
- the reversible nature of the disulfide bridges to enable easy wash-off of the gel was established in-vitro and in-vivo using a solution of reducing agent like Glutathione.
- concentration of Glutathione was varied from 1-5% w/v in phosphate buffer pH 8.
- the hydrogels having the varied compositions are shown in Table 16 and they showed a reversible nature of the disulfide linkages resulting in the hydrogel to convert from gel to sol.
- the hydrogel having a composition 6% w/v of 8-arm-PEG-SH and 8-arm-PEG-S-TP was sprayed on the mice and thereafter the hydrogel was washed off using a solution of Glutathione having a concentration 5% w/v as shown in FIG. 10 .
- the composition By exposure to or application of a reducing agent, such as cysteine or glutathione, to the cross-linked hydrogel composition, the composition can be completely dissolved and washed away.
- a reducing agent such as cysteine or glutathione
- One of the embodiments of the present invention is to show the reversible nature of the cross-links and easy wash off of the gel. This is exemplified in the present invention using the normal skin of mice and using in vitro experiments ( FIG. 10 ).
- a preferred hydrogel composition of the subject invention must be strong and flexible. It should not dry out too fast nor swell/shrink excessively. These properties were tested using rheology instrumentation and manual inspection of the gel.
- One of the embodiments of the present invention comprises 0.2-5% w/v polyvinyl pyrolidone (PVP), 0.2-5% w/v cellulose derivatives, such as hydroxypropyl methylcellulose, hydroxypropyl cellulose, 0.5-5% v/v PEG(6000 Da) and 0.4-25% v/v glycerin.
- phospholipids such as soybean phospholipids, eggyolk phospholipids, lecithins, soy lecithins, sphingomyelins, phosphatidylcholine, phosphatidylglycerol, phosphatidic acid, phosphatidylethanolamine, phosphatidyl serines, and PEG-ylated phospholipids.
- this formulation When sprayed on a Petri dish, this formulation produces a gel that forms a uniform layer without running of excess liquid ( FIG. 9 ). The gel may be peeled from the dish without tearing. Furthermore, the gel appears to be useful for up to 4 days. Based on these physical properties, a gel having a formulation as shown in Tables 9 or 10 or 11 may serve as the entire wound dressing when applied to skin, obviating the use of a gauze bandage.
- Rheological measurements were performed on a 4 and 6% w/v gel of 8-arm PEG-SH cross-linked by H 2 O 2 using a Rheometrics rheometer using RSI orchestrator software at 37° C. with cone plate geometry (plate diameter of 25 mm, gap of 3 mm and 2° angle). Samples were equilibrated on the plate for 5 min to reach the running temperature before each measurement. All rheological determinations were made at least in triplicate for each hydrogel using separate samples. Rheological test parameters like storage/elasticity (G′) and loss (G′′) moduli were obtained under dynamic conditions of non-destructive oscillatory tests.
- G′ storage/elasticity
- G′′ loss
- the hydrogels of 8-arm Peg-SH and H 2 O 2 formed in phosphate buffer and phosphate buffer containing 2% w/v polyvinyl pyrrolidone, 5% v/v glycerin and 5% v/v polyethylene glycol (MW 600) was measured. The results for the rheology are shown in FIG. 15 .
- hydrogels of 8-arm PEG-SH cross-linked by 8-arm PEG-S-TP formed in phosphate buffer and phosphate buffer containing 2% w/v polyvinyl pyrrolidone, 5% v/v glycerin and 5% v/v polyethylene glycol (MW 600) was measured. The results for the rheology are shown in FIG. 16 .
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Life Sciences & Earth Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Materials Engineering (AREA)
- Epidemiology (AREA)
- Hematology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Dispersion Chemistry (AREA)
- Medicinal Chemistry (AREA)
- Organic Chemistry (AREA)
- Dermatology (AREA)
- General Chemical & Material Sciences (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Pharmacology & Pharmacy (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Medicinal Preparation (AREA)
- Polymers & Plastics (AREA)
Abstract
Described is a spray-on hydrogel comprising water-soluble PEG polymers that cross-link in situ to form a hydrogel such that the cross-links are reversible. The hydrogel can be useful as a drug delivery composition, wound dressing or surgery adjuvant. Polyethylene glycol polymer and cross-linker solutions are sprayed simultaneously through a common orifice. Cross-linking via formation of thioether or disulfide bonds is initiated upon mixing, providing rapid gelation. The hydrogel components can be derivatized with RGD peptides or analogs thereof to promote retention in/on a body compartment such as the skin, surface of the eye, or a mucosa such as the vaginal mucosa. The cross-links are reversed using a reducing solution enabling easy removal of the hydrogel by dissolution. Processes for preparation of the cross-linker, RGD derivatized PEG and RGD-linked agents are also disclosed.
Description
- The use of hydrogel-based wound dressings for the promotion of wound healing is accepted clinical practice for wounds that have low to medium exudate. These dressings are typically applied to the skin as an adhesive bandage. Hydrogel-based wound dressings are cross-linked polymer gels in sheet form, having a gauze or an impervious polymer backing with an adhesive component provided for skin adhesion. Examples include, hydropolymer dressings impregnated with petroleum gauze or having water-resistant permeable polyurethane backing, paste dressings containing zinc oxide and calamine, waterproof foam dressing made of polyurethane film, gauze-based stretchable dressing, alginate-based dressings, collagen-based dressings and silver dressings. Hydrogel sheets are available from several commercial sources, including Tegagel (3M), Vigilon (Bard), Clearsite (Conmed Corporation), AQUASORB (DeRoyal), FLEXDERM (Bertek), NU-GEL (Johnson & Johnson), and CURAGEL (Kendall). These adhesive gauze or patch products, however, remain intact and have the disadvantage of being difficult to remove when peeling off from the skin.
- Hydrogels have also been employed to increase ocular residence time and enhance bioavailability for drugs applied to the eye. The hydrogels were found to provide better tolerability and less blurring of vision than ointments. Hydrogels used for ocular application are either pre-formed gels or are formed in situ. The pre-formed gels comprise, for example, cellulose derivatives, such as hydroxyethyl cellulose, hydroxymethyl cellulose, carboxymethyl cellulose; polyacrylic acids; cross-linked acrylic acid derivatives (carbomer); polyarcylamides; carbophil; gelatin; hyaluronic acid; polyvinyl alcohol; polyvinyl pyrrolidone; or xanthan gum.
- The in situ-forming gels typically comprise cellulose acetate phthalate, polaxomers, ethylene diamine derivative of polaxamine; psuedolatexes prepared by the incorporation of pilocarpine in cellulose acetate phthalate; various copolymers, such as PEO-PLLA and PEG-PLGA-PEG; cellulose acetophalate latex; Gelrite; carbopol; Matrigel; polyethylene oxide, polyoxypropylene, or gellan gum. However, most require a high (>20%) polymer concentration for in situ gel formation. Thermally gelling polymers (Poloxamer, Pluronics, PEO-PLLA diblock copolymer, PEG-PLGA-PEG triblock copolymer, and Matrigel) have a disadvantage of gelling before administration due to temperature change during packaging or storage, and can require manipulation of the temperature before administration. Furthermore, many of these polymers (e.g., Poloxamer and Pluronics) form a hydrogel which is a viscous, but still flowing solution and therefore are not readily applicable for use at a particular site on body surfaces.
- An in situ gelling polyvinyl alcohol (PVA)-based, fast cross-linking hydrogel system in the form of a spray, and suitable as a wound dressing, has been disclosed by Bohl Masters, et al., Wound Repair and Regeneration 10 (5), 286-294, 2002; and Bourke, et al., AAPS PharmSci 2003; 5(4) article 33. The PVA is functionalized by reacting with the acrylamide derivatives and are cross-linked by UV irradiation. The hydrogel provides a protective barrier on the skin but the cross-linking is irreversible. Accordingly, these hydrogel systems are not readily soluble and have to be peeled off to be removed them from the site.
- A hydrogel which can form in situ and is readily soluble, i.e., has reversible cross-linkage, can provide certain advantages as a wound dressing or drug delivery device at the site of application. These advantages include being relatively easy and painless to remove from the site of application, as well as being highly conformable to the site of application, such as a wound, the eye, or surrounding tissue. In addition, an in situ-forming hydrogel that can be administered as a spray can provide an advantageous method of application to the site. A spray-on gel for the topical delivery of drug to the eye, and having the drug or drugs linked to RGD peptides, which exhibit adhesive properties, can enhance the ocular residence time for the linked drugs.
- The subject invention concerns compositions, drug delivery devices and methods relating to in situ-forming hydrogels useful to form a protective covering over a wound or damaged area of the skin, such as cut or abrasion, a surgical site, or a blistered surface resulting from disease or trauma, such as a burn. An in situ-forming hydrogel composition of the subject invention can also be useful as a carrier for a drug or drugs administered by topical or transdermal application, including ocular application. The hydrogels of the subject invention can be adherent, i.e., the composition, itself, can have adherent or adhesive properties.
- Thus, the subject invention concerns a wound or ocular dressing comprising a first component comprising a hydrophilic polymer having a sulfhydryl, thiol, or mercaptan moiety; and a second component comprising a cross-linker, said cross-linker forming reversible cross-linkages with the hydrophilic polymer. The first and second components form a material that adheres to skin of a mammal and acts as a wound dressing. Preferably, the polymer is polyethylene glycol, and more preferably, the polyethylene glycol comprises a sulfhydryl, thiol, or mercaptan moiety to form disulfide bonds. The composition can also include a peptide, such as RGD peptide, the RGD peptide preferably being derivatized to the polyethylene glycol component. The composition can alternatively or additionally include a drug or combination of drugs or a growth factor.
- The subject invention also concerns a method of treatment for delivering a drug to a corneal surface of an eye. This method comprises the steps of:
-
- a. providing a reversibly cross-linked hydrogel composition of claim 14, and
- b. removing the hydrogel by dissolving said cross-links in the hydrogel composition.
- Removal of the hydrogel dressing preferably comprises reversing the cross-links using a reducing agent wherein the reducing agent is preferably cysteine or derivatives thereof, cysteine ethyl ester, cysteine methyl ester, gluthatione, cysteine hydrocholoride, dithiothretol, N-Ethylmalemide, phosphine derivatives tetrakis-hydroxymethyl phosphonium chloride and tris-diethylaminomethyl phosphine trialkylphosphine agents, such as Tris[2-carboxyethyl] phosphine and mercaptoethanols, 2,3-dimercapto-1-propanol, dinitrobenzoic acid, a thiol, a mercaptan, a sulfite or bisulfite or ammonium or sodium salts thereof, thioglycolic acid, thiolactic acid, cysteine, thioglycerol, thioglycolic hydrazide, thioglycolamide, glycerol monothioglycolate, beta-mercapto-propionic acid, N-hydroxyethyl mercapto-acetamide, N-methyl mercapto-acetamide, beta-mercapto-ethylamine, beta-mercapto-propionamide, 2-mercapto-ethanesulfonic acid, dimercapto-adipic acid, dithiothreitol, homocysteinethiolactone, and a polythiol derivative formed by the addition of cysteamine onto a maleic anhydride-alkylvinylether copolymer, and is most preferably glutathione or cysteine.
- The subject invention further includes a method of preparing a cross-linked hydrogel composition for application to the skin, said method comprising:
-
- a. providing a polymer in solution,
- b. providing in a separate solution a cross-linker that forms reversible cross-links,
- c. administering both solutions concomitantly from at least one nozzle permitting mixing of the polymer and cross-linking solutions in order to provide rapid gelation of the reversibly cross-linked hydrogel at the site of administration.
- A preferred embodiment of a composition of the subject invention comprises novel cross-linkers, such as RGD-derivatized PEG, and further can comprise RGD-linked drug. A hydrogel composition of the subject invention can comprise additional components or ingredients, including polyvinylpyrrolidone (PVP), propylene glycol, low molecular weight PEG (<6000 Da), glycerin, or cellulose derivatives such as hydroxypropyl cellulose, hydroxylpropyl methylcellulose, methylcellulose, or the like, as necessary to provide desired properties for the hydrogel in accordance with the functionalities as recognized in the art.
- The polymeric PEG component of the subject composition preferably comprises a sulfhydryl, thiol, or mercaptan moiety capable of forming a reversible disulfide bond or bridge for cross-linkage of the polymer. A preferred composition of the hydrogel according to the subject invention comprises a thiol-terminated PEG and several substances that are useful for cross-linking the thiol groups. Alternately, a PEG having a maleimide, thiopyridine or vinylsulfone termination can be used. At physiological pH and temperatures, cross-linking of PEG into a hydrogel can occur in about 1-3 minutes. The use of PEG offers several other advantages, including its chemoselective properties, its capability to form reversible and non-reversible cross-links, its free thiol group for covalently linking to drug, its property of blocking proteolytic enzymes and immune system components that can cause an inflammatory reaction, and its commercial availability in numerous forms.
- More preferably, the sulfhydryl or thiol or mercaptan-terminated PEG can reversibly bond to a sulfur-terminated moiety of a peptide, such as a peptide comprising the amino acid chain Arg-Gly-Asp (RGD) or a sulfur-containing amino acid, such as Cys. Thus, one preferred peptide used in accordance with the subject invention comprises Cys attached to the Asp amino acid of the RGD peptide. Use of a PEG-based polymer allows the hydrogel to be functionalized using these peptides to enhance the wound healing and the adhesive properties of the gel. Peptides typically used for this purpose include those having the sequence ‘Arg-Gly-Asp,’ or RGD, in cyclic or linear form. The heretofore undisclosed PEG polymers derivatized to include RGD peptide useful as cross-linker in a spray-on gel can advantageously provide bioadhesive and wound-healing properties to the formed hydrogel.
- In a preferred wound dressing embodiment of the subject invention, the PEG and cross-linker can be provided as separate solutions, being mixed during administration to the site, for example, provided as streams of solutions from separate sources or containers and administered simultaneously, allowing the gel and cross-linker solutions to mix together during administration, so that the cross-linked gel matrix sets in less than 30 minutes, and preferably less than 10 minutes. The disulfide bonds of the resultant cross-linked hydrogel matrix can provide the support to maintain the integrity of the gel, as well as the capability to adsorb into the gel the exudate from the wound or other site. The cross-linking within the polymeric composition can be readily reversed using a reducing agent. Such spray-on hydrogels having reversible cross-links for advantageous application to skin have not been previously disclosed. A preferred object of the invention is to provide enhanced patient compliance for a wound dressing by applying a hydrogel having reversible cross-links, e.g., cross-links containing disulfide bridges, so that the hydrogel wound dressing can be readily washed off by dissolution of the hydrogel rather than physical removal, such as peeling off, of the intact dressing.
- The drugs and other active components used in accordance with the subject invention can be dissolved or dispersed in the cross-linked polymeric matrix. Drugs which can provide anesthetic, antimicrobial, or wound healing properties are preferred for use in an embodiment directed to a protective covering for a wound. Alternatively, the drugs can be linked to an RGD peptide derivatized onto the PEG polymer.
- Another feature of the present invention is that hydrogel, cross-linked with RGD derivatized PEG cross-linker, can provide dermal retentive properties when applied to the skin, thereby providing prolonged release properties for the drug released from the hydrogel. This embodiment of a hydrogel of the subject invention can also enhance the cell adhesion of the drugs onto a corneal surface.
- The subject invention preferably comprises a hydrogel which is formulated to be applied or administered, preferably as a liquid and more preferably as a spray, wherein the formed gel comprises a reversibly cross-linked polymeric matrix or network. The cross-linking component is also preferably formulated as a liquid and more preferably as a spray. The hydrogel and cross-linking components are therefore preferably applied concomitantly as separate liquids and more preferably administered as a spray wherein the two liquids are mixed during the application thereof.
- By comprising a reversible cross-link, the hydrogel is soluble, and can be dissolved and easily removed from the site rather than requiring the gel to be removed intact, e.g., peeled, from the skin. More preferably, the spray formulation for the gel of the subject invention comprises cross-linked, water-soluble polyethylene glycol (PEG) polymers. PEG is advantageously a hydrogel-forming component that is well known for its safe and non-toxic properties.
- It is yet another object of the present invention to provide a rapidly gelling hydrogel network which can be sprayed into the eye to treat inflammation, allergic response or to treat infection. Further, the spray gel can be applied onto a wound or other traumatized area of the skin to aid the healing process. Preferably, gelation should occur in less than 30 minutes, more preferably within about 10 minutes, and most preferably in less than about 4 minutes. It is still another object of the invention to provide an in situ-forming hydrogel which results in gelation in less than one minute.
- It is another object of the present invention to provide a spray-on hydrogel system comprising a dual-source nozzle, such as a dual barrel syringe or a pressurized spray can, capable of concomitant spraying of a stream of polymeric solution and a stream of cross-linker solution. This spray-on hydrogel system can provide advantageous topical delivery to the eye or skin of an in situ-forming hydrogel containing a drug or drugs.
- A further object of the invention is to provide a controlled-release drug delivery system comprising a hydrogel wherein the drug or drugs are delivered from or through the hydrogel composition for a sustained or extended period of time. Preferably, the drug or drugs can be linked to RGD peptides incorporated into a PEG-based hydrogel. Linkage of drug to the RGD peptide component of the hydrogel can increase residence time in the ocular region or on the skin cells to increase their residence time at the site of application, thereby providing relatively high local concentrations and prolonged release and action of the drug or drugs.
- Yet another object of the invention is to provide a kit for applying a wound or ocular dressing or a hydrogel capable of delivering drug to the site of application. The kit can comprise a polymer-forming composition and a reversible cross-linking composition, wherein the polymer-forming and cross-linking compositions can be mixed to form a reversible, cross-linked hydrogel which rapidly gels to form a wound dressing or drug delivery device at the site of application. The kit can also include a composition containing drug, growth factor or other wound-healing enhancer, either separate from the polymer-forming composition and the cross-linking composition, or drug can be incorporated into either of these compositions. In addition, the kit can comprise a separately contained reducing agent to reverse the cross-linkage of the formed hydrogel, thereby providing a means for dissolving the hydrogel for its easy removal from the site without having to remove the hydrogel intact.
-
FIG. 1 depicts formation of disufide bridges (cross-links) leading to hydrogel based on 8-armPEG-SH and 8-armPEG-S-TP (thiopyridine). -
FIG. 2 depicts formation of disufide bridges (cross-links) leading to hydrogel based on 4-armPEG-SH and 4-armPEG-S-TP (thiopyridine). -
FIG. 3 depicts formation of disufide bridges (cross-links) leading to hydrogel based on 8-armPEG-SH and 4-armPEG-S-TP (thiopyridine). -
FIG. 4 depicts formation of disufide bridges (cross-links) leading to hydrogel based on 4-armPEG-SH and 8-armPEG-S-TP (thiopyridine). -
FIG. 5 depicts formation of disufide bridges (cross-links) leading to hydrogel based on 4-armPEG-SH and H2O2 -
FIG. 6 depicts formation of disufide bridges (cross-links) leading to hydrogel based on 8-armPEG-SH and H2O2. -
FIG. 7 depicts formation of thioether bonds (cross-links) leading to hydrogel based on 8-armPEG-SH and BMPEO3. -
FIG. 8 depicts formation of thioether bonds (cross-links) leading to hydrogel based on 8-armPEG-SH and BMPEO2. -
FIG. 9 depicts formation of hydrogel from a dual barrel syringe containing a polymer component and a cross-lining component in the respective barrels. -
FIG. 10 depicts removal of the gel using the reducing agent solution to cleave the disulfide bonds. -
FIG. 11 shows the DSC results for the melting of the 8-arm PEG-S-TP product. -
FIG. 12 depicts attaching the RGD peptide on the 8-arm-Peg-SH. -
FIG. 13 shows the DSC results for the melting of the RGD attached at 5 sites of 8-arm PEG-S-TP. -
FIG. 14 shows the DSC results for the melting of the RGD attached at 2 sites of 8-arm PEG-S-TP. -
FIGS. 15A and 15B shows the Rheology of hydrogels of 8-arm Peg-SH and H2O2 in buffer (A) and Rheology of hydrogel with additives (B). -
FIGS. 16A and 16B shows the Rheology of hydrogels of 8-arm Peg-SH and 8-arm Peg-S-TP in buffer (A) and Rheology of hydrogel with additives (B). -
FIG. 17 shows the ESI-MS spectrum of the Olvanil-Cysteine product. -
FIG. 18 shows the MALDI-TOF spectrum of the PEG-Olvanil-Cysteine product. - The subject invention concerns a composition comprising a drug or other active ingredient, a polymeric hydrogel-forming component and a cross-linker, providing in situ formation of the hydrogel when sprayed topically onto an area of the body. The subject hydrogel composition advantageously has bioadhesive properties and reversible cross-links.
- A preferred embodiment of the subject composition comprises a poly(ethylene glycol), or PEG, hydrogel that, when applied topically, provides an adhesive gel which adheres well to a body surface such as the cornea or the skin. Further, the subject composition can provide modulated drug release from the hydrogel so that drug can be released over a prolonged period of time, e.g., several hours or days, or up to about a week. The hydrogel composition of the subject invention preferably comprises RGD peptide-derivatized components, including (a) RGD peptide linked to the PEG to provide cell adhesive and wound-healing properties for the composition, or (b) RGD peptide linked to a drug or drugs used in the compositions to provide increased retention of drug at site of application and for prolonged release of drug to promote or enhance wound healing. By “RGD peptide”, it is meant a peptide comprising RGD.
- In a preferred use, the polymeric hydrogel and cross-linker are formulated as separate solutions and concomitantly introduced, e.g., sprayed, onto the target site such that the polymer and cross-linker solutions adequately mix to form in situ a cross-linked hydrogel network or matrix. Active drug or drugs can be incorporated into one or both of the solutions, and are preferably linked to the hydrogel forming polymer through an RGD peptide linkage wherein the RGD peptide is incorporated or derivatized into the polymer. The cross-linked hydrogel so formed can provide a protective barrier on an injured or affected area, thereby serving as a wound dressing, or can provide a composition for topical drug delivery.
- Preferably, the hydrogel base comprises PEG (polyethylene glycol) or PEG derivatives. A hydrogel formed from PEG is advantageously flexible, elastic and strong, enabling attachment on the eye, skin and or injured parts thereof. In addition, certain derivatized PEGs, such as PEGs derivatized with an RGD peptide, can enhance wound healing, anchor onto the injured site and deliver the drugs for extended periods of time ranging from hours, to days, or up to about a week. A PEG-based hydrogel is highly permeable, allowing diffusion of incorporated drugs, salts, water and gases. Satisfactory gel formation can be achieved using a ratio of polymer to cross-linker from about 5:1 to about 1:5. The concentration of PEG can be varied from about 2% to about 30% (w/w) in the hydrogel, and in certain embodiments is preferably about 8% to about 10%. The polymer and cross-linker are preferably dissolved in buffer in the pH range 4-9 and preferably in the pH range 5-8 to obtain the gels.
- The hydrogel composition, such as PEG-based composition, can provide a platform technology suitable for use with various types of drugs to be delivered. The drugs can be either physically entrapped or modified drugs with cleavable bonds can be physically incorporated or covalently linked into the hydrogel to provide controlled release, which is otherwise not possible for highly hydrophilic drugs which traverse easily through the gel.
- A composition according to the present invention can also be applied to skin for burns associated with fire, sunburn and chemical irritants, as well as physical injuries such as bed sores by providing a scaffold for seeding and repair of the damaged skin. A distinctive feature of the hydrogel of the subject invention is the formulation of reversible cross-linking such that the hydrogel matrix can be dissolved and readily washed off. This feature can advantageously minimize disruption of newly formed skin when removing the reversibly cross-linked hydrogel dressing, offering advantages over the typical gel bandages, which are required to be physically removed intact, e.g., by peeling off of the dressing, which can cause discomfort or further trauma to the wound site.
- The hydrogel composition can further comprise one or more drugs for delivering drug for treatment at the site. The drug or drugs can be physically entrapped within the matrix of the formed hydrogel or can be covalently linked to hydrogel, such as by the RGD peptide. Such drug or drugs can be wound healing enhancers, such as RGD peptides, antiseptics or antibiotics, anti-inflammatories, anesthetics, pain relievers, or drugs useful for in situ treatment, such as drugs for treating glaucoma at an ocular site, and growth factors. These drugs include, but are not limited to lidocaine, benzocaine, butamben, dibucaine, oxybuprocaine, pramoxine, proparacaine, proxymetacaine, novocaine, procaine, tetracaine, doxycycline, minocycline, oxytetracycline, sancycline, dedimethylamino tetracycline, indomethacin, diclofenac, ibuprofen, naproxen, ketoprofen, dexamethasone, a vallinoid, olvanil, capsaicin, benzalkonium chloride, an antiglaucoma medication, pilocarpine, timolol, levobunolol, betaxolol, or carbacol. The invention is not limited to the use of the drugs mentioned above and can be extended to other therapeutic agents which aid in wound healing processes when used for topical skin delivery or drugs used in ocular treatments, such as drugs used to treat glaucoma.
- Alternatively, or in addition to comprising a drug or drugs, a hydrogel of the subject invention can include a growth factor for promoting wound healing. The growth factors useful in accordance with the subject invention include the cytokines such as epidermal growth factor (EGF), including all members of the EGF family of proteins having one or more repeats of the conserved amino acid sequence: CX7CX4-5CX10-13CXCX8GXRC (where X represents any amino acid), transforming growth factor alpha (TGF-alpha), Transforming Growth Factor beta (TGF-b), keratinocyte growth factor (KGF-2), fibroblast growth factor fibronectin, fibrinogen, Granulocyte-Monocyte Colony Stimulating Factor (GM-CSF) and platelet-derived growth factor (PDGF).
- The subject invention preferably comprises a hydrogel which is formulated to be applied or administered, preferably as a liquid and more preferably as a spray, wherein the formed hydrogel comprises a reversibly cross-linked polymeric matrix or network. By comprising a reversible cross-link, the hydrogel is soluble, and can be dissolved and easily removed from the site rather than requiring the gel to be removed intact, e.g., peeled, from the skin. The cross-linking within the polymeric composition can be readily reversed using a reducing agent. For purposes of the subject invention, substances that have the ability to reduce other substances are said to be reductive and are known as reducing agents, reductants, or reducers. As is well-known in the art, reducing agents can transfer electrons to another substance, and is thereby, itself, oxidized. Because reducing agents “donate” electrons, they are also called electron donors.
- The reduction of a typical disulfide bond, as in an embodiment of the subject invention, proceeds by two sequential thiol-disulfide exchange reactions. Thiol-disulfide exchange is the principal reaction by which disulfide bonds are formed and rearranged. Disulfide reshuffling is a faster reaction. Thiol-disulfide exchange is a chemical reaction in which a thiolate group S— attacks a sulfur atom of a disulfide bond —S—S—. The original disulfide bond is broken, and its other sulfur atom is released as a new thiolate, carrying away the negative charge. Meanwhile, a new disulfide bond forms between the attacking thiolate and the original sulfur atom. The transition state of the reaction is a linear arrangement of the three sulfur atoms, in which the charge of the attacking thiolate is shared equally. The protonated thiol form —SH is unreactive, i.e., thiols cannot attack disulfide bonds, only thiolates. Typically, the thiolate of a redox reagent such as glutathione or dithiothreitol attacks the disulfide bond.
- The several reducing agents that either block or reverse the disulfide bridges forming the cross-linkage in accordance with the subject invention include cysteine, cysteine ethyl ester, and cysteine methyl ester, gluthatione, cysteine hydrocholoride, dithiothretol, N-Ethylmalemide, phosphine derivatives tetrakis-hydroxymethyl phosphonium chloride and tris-diethylaminomethyl phosphine trialkylphosphine agents, such as Tris[2-carboxyethyl] phosphine and mercaptoethanols, 2,3-dimercapto-1-propanol, and dinitrobenzoic acid. The reducing agents appropriate for use in accordance with the subject invention are not limited to these and can be any compound having thiols or mercaptan groups as well as sulfites and/or bisulfites. Mercaptans and thiols which can be used to reverse the disulfide linkages in a reversibly cross-linked hydrogel of the subject invention include thioglycolic acid, thiolactic acid, cysteine, thioglycerol, thioglycolic hydrazide, thioglycolamide, glycerol monothioglycolate, beta-mercapto-propionic acid, N-hydroxyethyl mercapto-acetamide, N-methyl mercapto-acetamide, beta-mercapto-ethylamine, beta-mercapto-propionamide, 2-mercapto-ethanesulfonic acid, dimercapto-adipic acid, dithiothreitol, homocysteinethiolactone, cysteine derivatives, and polythiol derivatives formed by the addition of cysteamine onto a maleic anhydride-alkylvinylether copolymer. The sulfites and/or bisulfites which can be used are the sodium and ammonium salts.
- Preferred reducing agents of the present invention are cysteine or cysteine derivatives and glutathione. Various concentrations of these agents will be effective in degrading the hydrogel matrix. The higher the concentration, the faster the degradation. However, the lowest effective concentration is preferably used in order to minimize adverse tissue reactions.
- A hydrogel composition of the present invention is further suitable for application as an ocular gel. A hydrogel composition of the subject invention can comprise an RGD-containing peptide having bioadhesive properties, linked to the polymeric (e.g., PEG) component for enhancing the adhesion to the cells. Further, RGD-linked drugs incorporated in the gels provide prolonged ocular residence times, enhancing the ocular bioavailability of the drugs.
- Examples of the compositions, components thereof, and properties of embodiments of the subject invention are provided hereinbelow
- The hydrogel network or matrix composition is obtained by formation of disulfide bridges or formation of thioether bonds in the PEG (polyethylene glycol) having a thiol group (reduced sulfhydryls groups ‘SH’) at each terminus. The cross-linking of PEG with thiol terminal groups is achieved by reacting the same with H2O2, maleimide cross-linkers (like bis-maleimido di/triethylene glycol derivatives eg. BMPEO2 (bis-maleimido diethylene glycol) and BMPEO3 (bis-maleimido triethylene glycol) BMOE (Bis-Maleimidoethane), BMH (bismaleimidohexane)) or PEG having a thiopyridine (TP) groups at the terminus or PEG having vinyl sulfone terminal groups or any other compound capable of forming the disulfide or thioether bonds with ‘SH’ terminated PEGs. The PEG polymers having thiol terminus groups include 2-, 3-, 4-, 8-arm or multiple arm thiol PEGs in the molecular weight range of 2,000 to 100,000 Da. Alternately the hydrogels can be obtained by cross-linking the PEG having a maleimide or thiopyridine terminated groups with compounds having thiol functionality.
- A. Thiol-Terminated Polymer and Hydrogen Peroxide Cross-Linker
- One of the embodiments of the present invention includes the gels based on thiol terminated PEGs (e.g., 8-arm-PEG-SH) cross-linked with H2O2. The in situ forming hydrogel was obtained by spraying the solution of the thiol terminated PEG and the cross linker: H2O2, in
phosphate buffer pH 8 from a dual barrel syringe to obtain the hydrogel. - Formulations according to the subject invention, having various concentrations of hydrogel component, mixed with varying volumes of 3% H2O2 as a cross-linker, and their relatively rapid gelling time, are shown in Tables 1-3.
-
TABLE 1 Hydrogel (6% w/v) with H2O2 as cross-linker pH = 8 8 PBS Equiv- Arm- Buffer 3 % H 202alence of Gelling PEG- Volume Volume H 202 for Excess of Time SH In μL In μL one SH H 202 in Sec 10 mg 166 μL 33.2 μL 6.6 5.6 30 (0.9 μL H202) 10 mg 166 μL 16.6 μL 3.3 2.3 33 (0.45 μL H202) 10 mg 166 μL 8.3 μL 1.65 0.65 36 (0.22 μL H202) 10 mg 166 μL 4.15 μL 0.82 (−0.18) 40 (0.11 μL H202) -
TABLE 2 Hydrogel (3% w/v) with H2O2 as cross-linker pH = 8- 8 PBS arm- Buffer 3 % H 202Equiv- PEG- Volume Volume alence of Excess of Gelling SH In μL In μL H202 H 202Time 5 mg 166 μL 33.2 μL 13.2 12.2 32 Sec (0.9 μL H202) 5 mg 166 μL 16.6 μL 6.6 5.6 50 Sec (0.45 μL H202) 5 mg 166 μL 8.3 μL 3.3 2.3 68 Sec (0.22 μL H202) 5 mg 166 μL 4.15 μL 1.65 0.65 2.4 min (0.11 μL H202) -
TABLE 3 Hydrogel (1.5% w/v) with H2O2 as cross-linker pH = 8- 8 PBS arm- Buffer 3 % H 202Equiv- PEG- Volume Volume alence of Excess of Gelling SH In μL In μL H202 H 202Time 2.5 mg 166 μL 33.2 μL 26.4 25.4 38 Sec (0.9 μL H202) 2.5 mg 166 μL 16.6 μL 13.2 12.2 110 Sec (0.45 μL H202) 2.5 mg 166 μL 8.3 μL 6.6 5.6 4.20 min (0.22 μL H202) 2.5 mg 166 μL 4.15 μL 3.3 2.3 6.0 min (0.11 μL H202) - B. Thiol Terminated Polymer and Maleimide Cross-Linker.
- Another embodiment of the present invention includes hydrogels of thiol terminated PEGs (e.g. 8-arm-PEG-SH) cross-linked with maleimide cross-linkers. The in situ forming hydrogel was obtained by mixing the solutions of the thiol terminated PEG and the cross-linker having a maleimide termination BMPEO3 (bis-maleimido triethylene glycol) in phosphate buffer pH 5.38 and 7.4 to obtain the hydrogels. The hydrogel forms by the formation of the thioether bonds and gels almost instantaneously as shown in Table 4.
-
TABLE 4 Hydrogel (4% w/v) with BM(PEO)3 as cross-linker 8-Arm- BM(PEO)3 PB Glycerin Gelling S. No PEG-SH wt pH % Time 1 8 mg 2 mg 5.38 25 10 sec 2 8 mg 2 mg 5.38 50 10 sec 3 8 mg 2 mg 7.4 25 2 sec 4 8 mg 2 mg 7.4 50 2 sec - C. Sulfhydryl-Terminated Polymer and Thiopyridine Cross-Linker.
- Yet another embodiment of the present invention is a gel based on the 4-arm-PEG-SH and 8-arm-PEG-SH cross-linked with 4-arm and 8-arm-PEG respectively having thiopyridine terminal groups. The thiol terminated PEG (4 and 8-arm PEG-SH) is treated with three fold excess of dithiodipyridine (aldrithiol) in alcohol under mild acidic conditions overnight at room temperature. The product, a thiopyridine terminated PEG (4 arm PEG-S-TP and 8-arm PEG-S-TP) so obtained is purified using the size exclusion chromatography. This PEG-S-TP product is used for the gel formation.
- NMR for the Thiopyridine Terminated PEG.
- PEG-SH: 1H-NMR (CDCl3, 500 MHz) δ 3.50 (t, 2H, J=2, 4 Hz, OCH2) 3.60-3.67 (m, nH, OCH2—CH2—O)
PEG-S-TP: 1H-NMR (CDCl3, 500 MHz) δ 3.17 (t, 1H, J=2, 4 Hz, —CH—S-TP) 3.50 (t, 2H, J=2, 4 Hz, OCH2) 3.60-3.67 (m, nH, OCH2—CH2—O), 3.8 (t, 1H, J=2, 4 Hz, —CH—S-TP) 7.64 (t, 1H, J=2.4 Hz, Ar) 8.40 (t, 1H, J=2.6 Hz, Ar) 8.47 (d, 1H, J=3 Hz, Ar) 8.62 (d, 1H, J=3 Hz, Ar). - The in situ forming hydrogel was obtained by spraying the solution of the thiol terminated PEG and the cross-linker: having a thiopyridine termination developed in-house, from a dual barrel syringe to obtain the hydrogel. The solution of polymer and cross-linker were made in
phosphate buffer pH 8. The formulation comprising PEG with 4 and 8 terminal thiol groups, respectively, was used for the formation of hydrogel as shown in Table 5. The thiopyridine terminated cross-linker was obtained from the 4- and 8-arm thiol terminated PEG, respectively, as also shown in Table 5. -
TABLE 5 Hydrogel with PEG-S-TP as cross-linker Concen- tration of polymer Gelation S. No Composition (% w/v) Ratio Time 1 4-arm-PEG-S- TP + 8 1:1 15-30 sec 4-arm-PEG-SH 6 1:1 15-30 sec 5 1:1 15-30 sec 2 8-arm-PEG-S- TP + 8 1:1 15-30 sec 8-arm-PEG-SH 6 1:1 15-30 sec 5 1:1 15-30 sec 3 8-arm-PEG-S- TP + 8 1:1 15 sec 4-arm PEG-SH 6 1:1 15 sec 5 1:1 15 sec 4 4-arm-PEG-S- TP + 8 1:1 15 sec 8-arm PEG-SH 6 1:1 15 sec 5 1:1 15 sec - The concentration of the polymer solution and the cross-linker was varied to yield the hydrogel almost instantaneously, as shown in Table 6. The hydrogel is obtained by the formation of the disulfide bridges either inter or intramolecular in the thiol terminated PEG.
-
TABLE 6 Hydrogel with PEG-S-TP as cross-linker (different ratios) 8-arm PEG-SH (compound A) + 8-arm PEG-S-TP (compound B) Compound Ratio Gelling S. No % w/v A: B time 1 8 1:1 20-30 sec 2 8 2:1 20-30 sec 3 8 1:2 20-30 sec 4 6 1:1 30 sec 5 6 2:1 30 sec 6 6 1:2 30 sec 7 5 1:1 30 sec 8 5 2:1 30 sec 9 5 1:2 30 sec - To enhance the adhesion of the gel to eye and the skin the cell adhesive component is incorporated in the gel. Accordingly, one of the embodiments of the present invention discloses the use of the peptide sequence, Arg-Gly-Asp (RGD), which is naturally present in many proteins involved in adhesion of cells to other cells and to basement membrane. As a result of better contact, this provides better transfer of a drug from the gel to the site of application. Furthermore, the presence of RGD sequences can be recognized by cellular receptors, thereby serving as attachment sites on the corneal epithelial cells or keratinocytes cells on the skin. The RGD is known to accelerate skin and wound repair.
- RGD peptide comprising the ‘Arg-Gly-Asp’ sequence, such as the linear peptide Arg-Gly-Asp-Cys, Gly-Arg-Gly-Asp-Ser, Gly-Arg-Gly-Asp-Ser-Pro, or as the cyclic peptide Cyclo-Arg-Gly-Asp-Try-Lys, were used to enhance the adhesion of the gel on the cells and were synthesized as described below.
- Synthesis of the RGD Derivatized PEG Cross-Linker:
-
- Step 1: The thiol terminated PEG (8-arm PEG-SH) (1 g) was treated with three fold excess of dithiodipyridine (aldrithiol) (3 g) in alcohol (methanol, 20 ml) under mild acidic conditions overnight at room temperature. The DSC results for the melting of the 8-arm PEG-S-TP product are shown in
FIG. 11 . - Step 2: The thiopyridine terminated PEG obtained from
Step 1 was reacted with the RGD peptide having Arg-Gly-Asp-Cys sequence (FIG. 12 ). The thiopyridine terminated PEG was reacted with the RGD peptides in alcohol under mild basic conditions. The amount of RGD is taken proportional to the sites at which it is required to be appended e.g., the 8-arm PEG-TP (200 mg) was reacted with 1 equivalent of RGD to appended the RGD on one arm (36 mg), and the 8-arm PEG-TP (200 mg) was reacted with 3 equivalents of RGD (108 mg) to appended the RGD on three arms. The DSC for the RGD-PEG-S-TP is shown inFIGS. 13 and 14 .
- Step 1: The thiol terminated PEG (8-arm PEG-SH) (1 g) was treated with three fold excess of dithiodipyridine (aldrithiol) (3 g) in alcohol (methanol, 20 ml) under mild acidic conditions overnight at room temperature. The DSC results for the melting of the 8-arm PEG-S-TP product are shown in
- The hydrogel was obtained by spraying the solution of thiol terminated PEG (8-armPEG-SH) and the cross-linker consisting of PEG having partial thiopyridine and RGD terminal groups (RGDC-8-armPEG-S-TP), in
phosphate buffer pH 8. The hydrogel composition is shown in Table 7. -
TABLE 7 Hydrogel compositions having Adhesive RGD peptide % w/v of polymers in PB Gelling S. No. Polymers (pH 8) Ratio time 1 8-armPEG- SH + 5 1:1 ~10 min 2 RGDC-8-armPEG-STP 6 1:1 ~10 min 3 (5arm) 8 1:1 ~6 min 4 8-armPEG- SH + 5 1:1 ~6 min 5 RGDC-8-armPEG-STP 6 1:1 ~5 min 6 (2arm) 8 1:1 ~5 min 7 8-armPEG- SH + 5 1:1 ~4 min 8 RGDC-8-armPEG-STP 6 1:1 ~3 min 9 (1arm) 8 1:1 ~3 min - In another embodiment of the invention, a spray-on hydrogel was obtained by spraying the solution of thiol terminated PEG (8-arm PEG-SH) and along with the cross-linker solution consisting of (a) thiopyridine terminated PEG (8-arm PEG-S-TP) and (b) PEG having partial thiopyridine and RGD terminal groups (RGDC-8-armPEG-S-TP). The solution of polymer and cross-linker were mixed in
phosphate buffer pH 8. The hydrogel results instantaneously. The hydrogel compositions are shown in Table 8. -
TABLE 8 Hydrogel compositions with Adhesive (RGD peptide) % w/v polymer A and (B + C) in Ratio Ratio Gelling Polymer A Polymer B Polymer C PB (pH 8) A:B + C B:C time 8-armPEG- RGDC-8- 8-arm PEG- 5 1:1 1:1 ~30-40 sec SH arm PEG- STP 6 1:1 1:1 ~30-40 sec STP (5arm) 8-armPEG- RGDC-8- 8-arm PEG- 5 1:1 1:1 ~30-40 sec SH arm PEG- STP 6 1:1 1:1 ~30-40 sec STP (2arm) 8-armPEG- RGDC-8- 8-arm PEG- 5 1:1 1:1 ~30-40 sec SH arm PEG- STP 6 1:1 1:1 ~30-40 sec STP (1arm) - A spray-on hydrogel was obtained by dissolving the thiol terminated PEG (4-arm-PEG-SH and 8-arm PEG-SH) and the thiopyridine terminated PEG (4-arm-PEG-S-TP and 8-arm PEG-S-TP) (Formulation in accordance with Example 1c), and an additive comprising a solution of polyvinyl pyrrolidone (PVP) in
phosphate buffer pH 8. The concentration of PVP was varied from 1.5-2% w/v as shown in Table 9. -
TABLE 9 Hydrogel Compositions with additive PVP Conc. Conc. Of Ratio of Of PVP polymers S. No Compositions polymers (% w/v) (% w/v) 1 4-arm-PEG-S-TP + 1:1 — 5 4-arm-PEG-SH 8-arm-PEG-S-TP + 1:1 — 5 8-arm-PEG-SH 8-arm-PEG-S-TP + 1:1 — 5 4-arm PEG- SH 2 4-arm-PEG-S-TP + 1:1 1.5 5 4-arm-PEG-SH 8-arm-PEG-S-TP + 1:1 8-arm-PEG-SH 8-arm-PEG-S-TP + 1:1 4-arm PEG-SH 3 4-arm-PEG-S-TP + 1:1 2 5 4-arm-PEG-SH 8-arm-PEG-S-TP + 1:1 8-arm-PEG-SH 8-arm-PEG-S-TP + 1:1 4-arm PEG-SH - Two spray-on hydrogels were obtained by dissolving the thiol terminated PEG (8-arm PEG-SH) and the thiopyridine terminated PEG (8-arm PEG-S-TP) (in accordance with a formulation of Example 1c) with additives comprising (a) a solution of 2% w/v polyvinylpyrrolidone (PVP) and 5% v/v of glycerin in
phosphate buffer pH 8, and (b) by solution of 2% w/v polyvinyl pyrrolidone (PVP), 5% v/v of glycerin and 5% v/v of polyethylene glycol (MW 600) inphosphate buffer pH 8. These are shown in Table 10. -
TABLE 10 Hydrogel Compositions with additive Glycerin and PEG (MW 600) S. No 1Conc. Of 8 arm PEG Conc. Of Ratio of (MW 20,000) Glycerin Compositions polymers (% w/v) (% v/v) 8-arm-PEG-S-TP + 1:1 6 5 8-arm-PEG-SH Dissolved in Phosphate buffer pH 8 containing PVP (2% w/v)) S. No 2Conc. Of Conc. Of PEG (MW 600) 8 arm PEG (% v/v) Ratio of (MW 20,000) (+Glycerin Compositions polymers (% w/v) 5% v/v) 8-arm-PEG-S-TP + 1:1 6 5 8-arm-PEG-SH Dissolved in Phosphate buffer pH 8 containing PVP (2% w/v) - A spray-on hydrogel was obtained by dissolving the thiol terminated PEG (4-arm-PEG-SH and 8-arm PEG-SH) and the thiopyridine terminated PEG (4-arm-PEG-S-TP and 8-arm PEG-S-TP) in a solution of polyvinylpyrrolidone (PVP) and hydroxypropyl methylcellulose (HPMC) in
phosphate buffer pH 8. The concentration of PVP was varied from 1.5-2% w/v as shown in Table 11. -
TABLE 11 Hydrogel Compositions with additives PVP and HPMC Conc. Conc. Conc. Of Ratio of Of PVP Of HPMC polymers S. No Compositions polymers (% w/v) (% w/v) (% w/v) 1 4-arm-PEG-S-TP + 1:1 1.5 0.5 5 4-arm-PEG-SH 8-arm-PEG-S-TP + 1:1 8-arm-PEG-SH 8-arm-PEG-S-TP + 1:1 4-arm PEG- SH 2 4-arm-PEG-S-TP + 1:1 2 1.0 5 4-arm-PEG-SH 8-arm-PEG-S-TP + 1:1 8-arm-PEG-SH 8-arm-PEG-S-TP + 1:1 4-arm PEG-SH - A. Lidocaine and Doxycycline Hyclate.
- The drugs Lidocaine and Doxycycline Hyclate were incorporated in the hydrogel by dissolving the drugs in the polymer solution (8-arm PEG-SH) as shown in Table 12. The drug incorporated polymer solution was cross-linked using the thiopyridine terminated PEG (8-arm PEG-S-TP) in
phosphate buffer pH 8. Also, The drug incorporated polymer solution was cross-linked using the thiopyridine terminated PEG (8-arm PEG-S-TP) in a solution of 2% w/v polyvinyl pyrrolidone (PVP) and 5% v/v of glycerin inphosphate buffer pH 8 as shown in Table 12. -
TABLE 12 Hydrogel Compositions with Doxycycline Hyclate, Lidocaine HCL and Benzalkonium Chloride Conc. Ratio of Of PEG Drugs S. No Compositions polymers (% w/v) % w/ v 1 8-arm-PEG-S-TP + 8-arm- 1:1 6 2.5% Lidocaine PEG-SH HCL and (Dissolved in phosphate 0.15% buffer pH 8) Benzalkonium HCL 2 8-arm-PEG-S-TP + 8-arm- 1:1 6 2.5% Lidocaine PEG-SH HCL and Dissolved in Phosphate 0.15 % buffer pH 8 containing Benzalkonium Other additives: HCL PVP (2% w/v) PEG Mw 600 (5% v/v) Glycerin (5% v/v) 3 8-arm-PEG-S-TP + 8-arm- 1:1 8 0.34% PEG-SH Doxycycline (Dissolved in phosphate hyclate buffer pH 8) 4 8-arm-PEG-S-TP + 8-arm- 1:1 8 0.34% PEG-SH Doxycycline Dissolved in Phosphate hyclate buffer pH 8 containingOther additives: PVP (2% w/v) PEG Mw 600 (5% v/v) Glycerin (5% v/v) - B. Doxycycline
- In another embodiment, the drug Doxycycline Hyclate was incorporated in the hydrogel by dissolving the drug in the polymer (8-arm-PEG-SH) solution in
phosphate buffer pH 8. The drug incorporated polymer solution was cross-linked using the H2O2 solution as shown in Table 13. -
TABLE 13 Hydrogel compositions cross-linked by H 202 with Doxycycline HyclateWt of pH = 8 PBS 8-arm- Buffer 3 % H 202Equiv- Doxycycline PEG-SH Volume Volume alent of Hyclate (mg) In μL In μL H 202 % w/ v 8 mg 200 μL 1.8 μL 0.5 0 8 mg 200 μL 1.8 μL 0.5 0.5 8 mg 200 μL 1.8 μL 0.5 0.25 8 mg 200 μL 1.8 μL 0.5 0.12 12 mg 200 μL 5.4 μL 0.5 0.5 12 mg 200 μL 5.4 μL 0.5 0.25 12 mg 200 μL 5.4 μL 0.5 0.122 12 mg 200 μL 5.4 μL 0.5 0.061 16 mg 200 μL 5.4 μL 0.5 0.5 16 mg 200 μL 5.4 μL 0.5 0.25 16 mg 200 μL 5.4 μL 0.5 0.122 - C. Indomethacin.
- The drug indomethacin was incorporated in the hydrogel by dissolving the drug in the polymer solution (8-arm PEG-SH) as shown in Table 14. The drug incorporated polymer solution was cross-linked using the thiopyridine terminated PEG (8-arm PEG-S-TP) in
phosphate buffer pH 8. Also, The drug incorporated polymer solution was cross-linked using the thiopyridine terminated PEG (8-arm PEG-S-TP) in a solution of 2% w/v polyvinylpyrrolidone (PVP) and 5% v/v of glycerin inphosphate buffer pH 8 as shown in Table 14. - Synthesis of the RGD Linked to Indomethacin
-
- Step 1: The RGD peptide having sequence Arg-Gly-Asp-Cys would be treated with three fold excess of dithiodipyridine (aldrithiol) in alcohol under mild acidic conditions overnight at room temperature, to obtain the protected peptide.
- Step 2: The indomethacin (1 eq) would be linked thiopyridine protected peptide (1 eq) in the presence of coupling agents coupling agents 4-dimethylaminopyridine and 1(3-dimethylaminopropyl) 3-ethylcarbodiimide (1 eq) in solution of dry dimethylformamide and using hydroxyl-terminated PEG as a spacer. The reaction would be carried out overnight at room temperature and the product would be separated by size exclusion chromatography using
Sephadex LH 60 packing. The indomethacin-PEG-RGD conjugate would be linked to the 8-arm PEG-SH through the disulfide bond formation at the cysteine terminal of the peptide in phosphate buffer (pH 7.4) with stirring overnight and the product would be obtained by lyophilization of the reaction mixture for 12-24 hrs.
-
TABLE 14 Hydrogel Compositions with Indomethacin Conc. Ratio of Of PEG Drugs S. No Compositions polymers (% w/v) % w/ v 1 8-arm-PEG-S-TP + 8- 1:1 6 0.4% arm-PEG-SH Indomethacin (Dissolved in phosphate buffer pH 8) 2 8-arm-PEG-S-TP + 8- 1:1 6 0.4% arm-PEG-SH Indomethacin Dissolved in Phosphate buffer pH 8containing Other additives: PVP (2% w/v) PEG Mw 600 (5% v/v) Glycerin (5% v/v) - D. Doxycycline Conjugated to RGD.
- Doxycycline-RGD-PEG was incorporated in the hydrogel by dissolving the same in the polymer solution (8-arm PEG-SH) as shown in Table 15. The drug incorporated polymer solution was cross-linked using the thiopyridine terminated PEG (8-arm PEG-S-TP) in
phosphate buffer pH 8. Also, The drug incorporated polymer solution was cross-linked using the thiopyridine terminated PEG (8-arm PEG-S-TP) in a solution of 2% w/v polyvinylpyrrolidone (PVP) and 5% v/v of glycerin inphosphate buffer pH 8 as shown in Table 15. The Doxycycline-RGD-PEG component was synthesized and the synthetic procedure is given below. - Synthesis of the RGD-Linked Drug Composition
-
- Step 1: The RGD peptide having sequence Arg-Gly-Asp-Cys was treated with three fold excess of dithiodipyridine (aldrithiol) in alcohol under mild acidic conditions overnight at room temperature. To obtain the protected peptide
- Step 2: The Doxycycline (1 eq) was linked thiopyridine protected peptide (1 eq) in the presence of coupling agents 4-dimethylaminopyridine and 1(3-dimethylaminopropyl) 3-ethylcarbodiimide (1 eq) in solution of dry dimethylformamide. The reaction was carried out overnight at room temperature and the product was separated by size exclusion chromatography using
Sephadex LH 60 packing. The PEG-ylation of Doxycycline-RGD conjugate was carried out using 8-arm PEG-SH in phosphate buffer (pH 7.4) with stirring overnight. The ratio of Doxycycline-RGD to 8-arm PEG-SH was taken (1:1 per arm). The Doxycycline-RGD-PEG so synthesized was obtained by lyophilization of the reaction mixture for 12-24 hrs.
- The different sizes of PEG (10-20 KDa) and with different number of thiol termination (2,4 and 8-arm Peg-SH) can be used. Further, the amount of Doxycycline-RGD can be taken proportional to the number of arms/sites at which it is required to be appended on the 4 or 8-arm PEG-SH.
-
TABLE 15 Hydrogel Compositions with Doxycycline-RGD-PEG Conc. Ratio of Of PEG Drugs S. No Compositions polymers (% w/v) % w/ v 1 8-arm-PEG-S-TP + 8- 1:1 6 Doxycycline- arm-PEG-SH RGD-PEG (Dissolved in (equivalent phosphate buffer pH 8) to 0.34%) 2 8-arm-PEG-S-TP + 8- 1:1 6 Doxycycline- arm-PEG-SH RGD-PEG Dissolved in (equivalent Phosphate buffer pH 8to 0.34%) containing Other additives: PVP (2% w/v) PEG Mw 600 (5% v/v) Glycerin (5% v/v) - The different sizes of PEG (10-20 KDa) and with different number of thiol termination (2,4 and 8-arm Peg-SH) can be used. Further, the amount of indomethacin-PEG-RGD can be taken proportional to the number of arms/sites at which it is required to be appended on the 4 or 8-arm PEG-SH.
- E. Olvanil.
- The drug olvanil was be incorporated in the hydrogel by dispersing the drug in the polymer solution (8-arm PEG-SH). The drug incorporated polymer solution was cross-linked using the thiopyridine terminated PEG (8-arm PEG-S-TP) in
phosphate buffer pH 8. Also, the drug incorporated polymer solution was cross-linked using the thiopyridine terminated PEG (8-arm PEG-S-TP), both the polymer and cross-linker were dissolved in a solution of 2% w/v polyvinylpyrrolidone (PVP) and 5% v/v of glycerin inphosphate buffer pH 8 as shown in Table 15. - The PEG-Olvanil-Cysteine was incorporated in the hydrogel by dissolving the same in the polymer solution (8-arm PEG-SH). The drug incorporated polymer solution was cross-linked using the thiopyridine terminated PEG (8-arm PEG-S-TP) in
phosphate buffer pH 8. Also, the drug incorporated polymer solution was cross-linked using the thiopyridine terminated PEG (8-arm PEG-S-TP) in a solution of 2% w/v polyvinylpyrrolidone (PVP) and 5% v/v of glycerin inphosphate buffer pH 8. - The PEG-Olvanil-Cysteine component was synthesized and the synthesis procedure is given below.
- Synthesis of the PEG-Olvanil-Cysteine
- Step 1: The 3-fold excess of Fmoc-Cysteine(S-Trt)-COOH was reacted with Olvanil in presence of diisopropylcarbodiimide under basic conditions by adding pyridine in dimethyl formamide. The formation of the Olvanil-Cysteine product was analyzed using ESI-MS as shown in
FIG. 17 . - Step 2: Olvanil-Cys(trt) ester was linked to 5 kDa-PEG-NHS in the presence of N N-Diisopropylethylamine by dissolving in dimethyl formamide by stirring overnight. The product so obtained was purified by Size exclusion chromatography using G-25 Sephadex beads. The formation of the product was analyzed using MALDI-TOF as shown in
FIG. 18 . - One of the embodiments of the present invention includes a therapeutic agent or drug, such as lidocaine (a topical anesthetic), benzalkonium chloride (a topical antiseptic), olvanil (an anti-inflammatory agent), doxycycline (an antibiotic), pilocarpine or protease inhibitors incorporated into the hydrogel composition.
- Formulations incorporating the drugs into the hydrogel composition are shown in Tables 12-15. For prolonged retention of drugs at the site of application, drugs linked to RGD peptide appended onto PEG were incorporated in the hydrogel composition, e.g., doxycycline as disclosed in Example 5. D. The RGD peptide, comprising the ‘Arg-Gly-Asp’ sequence, such as the liner peptide or the cyclic peptides are disclosed. The examples of the linear peptide include Arg-Gly-Asp-Cys, Gly-Arg-Gly-Asp-Ser, Gly-Arg-Gly-Asp-Ser-Pro, and the cyclic peptide include, Cyclo-Arg-Gly-Asp-Try-Lys but are not so limited, and can be extended to any peptide having the sequence ‘Arg-Gly-Asp’. These prodrugs are designed to provide cell adhesive and retentive properties to enhance binding to corneal epithelial cells or to the extracellular matrix of the injured skin for slow-release the active drug.
- The reversible nature of the disulfide bridges to enable easy wash-off of the gel was established in-vitro and in-vivo using a solution of reducing agent like Glutathione. The concentration of Glutathione was varied from 1-5% w/v in
phosphate buffer pH 8. The hydrogels having the varied compositions are shown in Table 16 and they showed a reversible nature of the disulfide linkages resulting in the hydrogel to convert from gel to sol. - The hydrogel having a composition 6% w/v of 8-arm-PEG-SH and 8-arm-PEG-S-TP was sprayed on the mice and thereafter the hydrogel was washed off using a solution of Glutathione having a
concentration 5% w/v as shown inFIG. 10 . -
TABLE 16 Evaluation of concentration of Glutathione required to reverse the disulfide cross-links in hydrogel Conc. Of Ratio of polymers Conc. of Glutathione (% w/v) S.No Composition polymers (% w/v) 1 3 5 1 4-arm-PEG-S-TP + 1:1 5 30 min 20 min 10-15 min 4-arm-PEG-SH 1:1 6 30 min 20-25 min 15 min 1:1 8 40-45 min 25-30 min 15-20 min 2 8-arm-PEG-S-TP + 1:1 5 40-45 min 20-25 min 15 min 8-arm-PEG-SH 1:1 6 Not investigated 20-25 min 15-20 min 1:1 8 Not investigated 30-35 min 15-20 min 3 8-arm-PEG-S-TP + 1:1 5 35-40 min 15 min 10-12 min 4-arm PEG-SH 1:1 6 Not investigated 15-20 min 10-12 min 1:1 8 Not investigated 15-20 min 10-15 min 4 4-arm-PEG-S-TP + 1:1 5 30-40 min 15 min 10-12 min 8-arm PEG-SH 1:1 6 Not investigated 15 min 10-12 min 1:1 8 Not investigated 20-30 min 15 min - By exposure to or application of a reducing agent, such as cysteine or glutathione, to the cross-linked hydrogel composition, the composition can be completely dissolved and washed away. One of the embodiments of the present invention is to show the reversible nature of the cross-links and easy wash off of the gel. This is exemplified in the present invention using the normal skin of mice and using in vitro experiments (
FIG. 10 ). - A preferred hydrogel composition of the subject invention must be strong and flexible. It should not dry out too fast nor swell/shrink excessively. These properties were tested using rheology instrumentation and manual inspection of the gel. One of the embodiments of the present invention comprises 0.2-5% w/v polyvinyl pyrolidone (PVP), 0.2-5% w/v cellulose derivatives, such as hydroxypropyl methylcellulose, hydroxypropyl cellulose, 0.5-5% v/v PEG(6000 Da) and 0.4-25% v/v glycerin. Other additives that can be included in the hydrogel composition include phospholipids such as soybean phospholipids, eggyolk phospholipids, lecithins, soy lecithins, sphingomyelins, phosphatidylcholine, phosphatidylglycerol, phosphatidic acid, phosphatidylethanolamine, phosphatidyl serines, and PEG-ylated phospholipids. When sprayed on a Petri dish, this formulation produces a gel that forms a uniform layer without running of excess liquid (
FIG. 9 ). The gel may be peeled from the dish without tearing. Furthermore, the gel appears to be useful for up to 4 days. Based on these physical properties, a gel having a formulation as shown in Tables 9 or 10 or 11 may serve as the entire wound dressing when applied to skin, obviating the use of a gauze bandage. - Rheological measurements were performed on a 4 and 6% w/v gel of 8-arm PEG-SH cross-linked by H2O2 using a Rheometrics rheometer using RSI orchestrator software at 37° C. with cone plate geometry (plate diameter of 25 mm, gap of 3 mm and 2° angle). Samples were equilibrated on the plate for 5 min to reach the running temperature before each measurement. All rheological determinations were made at least in triplicate for each hydrogel using separate samples. Rheological test parameters like storage/elasticity (G′) and loss (G″) moduli were obtained under dynamic conditions of non-destructive oscillatory tests. The hydrogels of 8-arm Peg-SH and H2O2 formed in phosphate buffer and phosphate buffer containing 2% w/v polyvinyl pyrrolidone, 5% v/v glycerin and 5% v/v polyethylene glycol (MW 600) was measured. The results for the rheology are shown in
FIG. 15 . - Additional rheological measurements were performed on a 5% w/v gel of 8-arm PEG-SH cross-linked by 8-arm PEG-S-TP using a Rheometrics rheometer using RSI orchestrator software at 37° C. with cone plate geometry (plate diameter of 25 mm, gap of 3 mm and 2° angle). Samples were equilibrated on the plate for 5 min to reach the running temperature before each measurement. All rheological determinations were made at least in triplicate for each hydrogel using separate samples. Rheological test parameters like storage/elasticity (G′) and loss (G″) moduli were obtained under dynamic conditions of non-destructive oscillatory tests. The hydrogels of 8-arm PEG-SH cross-linked by 8-arm PEG-S-TP formed in phosphate buffer and phosphate buffer containing 2% w/v polyvinyl pyrrolidone, 5% v/v glycerin and 5% v/v polyethylene glycol (MW 600) was measured. The results for the rheology are shown in
FIG. 16 .
Claims (46)
1. A wound dressing, comprising:
a first component comprising a hydrophilic polymer having a sulfhydryl, thiol, or mercaptan moiety; and
a second component comprising a cross-linker, said cross-linker forming reversible cross-linkages with the hydrophilic polymer;
wherein the first and second components form a material that adheres to skin of a mammal and acts as a wound dressing.
2. The wound dressing of claim 1 , wherein the polymer is polyethylene glycol.
3. The wound dressing of claim 2 , wherein the polyethylene glycol comprising the sulfhydryl, thiol, or mercaptan moiety forms disulfide bonds.
4. The wound dressing of claim 1 , wherein the composition further comprises a drug or a combination of drugs.
5. The wound dressing of claim 1 wherein the polyethylene glycol is derivatized to contain peptide comprising RGD.
6. The wound dressing of claim 5 wherein a drug or drugs are conjugated to the peptide comprising RGD.
7. The wound dressing of claim 5 wherein the peptide comprising RGD further comprises cysteine.
8. The wound dressing of claim 5 wherein the peptide comprising RGD is a linear peptide having an amino acid sequence selected from the group consisting of Arg-Gly-Asp-Cys, Gly-Arg-Gly-Asp-Ser, and Gly-Arg-Gly-Asp-Ser-Pro.
9. The wound dressing of claim 1 , wherein the cross-linker is selected from the group consisting of H2O2, a maleimide terminated polyethylene glycol, and a thiopyridine terminated polyethylene glycol.
10. The wound dressing of claim 9 wherein the maleimide terminated polyethylene glycol is selected from the group consisting of bis-maleimido diethylene glycol and bis-maleimido triethylene glycol.
11. The wound dressing of claim 9 wherein the thiopyridine terminated polyethylene glycol is obtained by reacting the 2-dithiopyridine or 4-dithiopyridine with thiol terminated polyethylene glycol.
12. The wound dressing of claim 1 , wherein the composition further comprises an additive selected from the group consisting of polyvinyl pyrrolidone, polyethylene oxide, cellulose derivatives such as methyl cellulose, hydroxypropyl methylcelluose, hydroxypropyl cellulose, humectants such as glycerin, propylene glycol, polyethylene glycols having molecular weight in the range 100-10000, propylene glycol derivatives, and phospholipids.
13. The wound dressing of claim 1 wherein the composition provides controlled release of said drug.
14. An ocular dressing, comprising:
a first component comprising a hydrophilic polymer having a sulfhydryl, thiol, or mercaptan moiety; and
a second component comprising a cross-linker, said cross-linker forming reversible cross-linkages with the hydrophilic polymer;
wherein the first and second components form a material that adheres to the ocular surface of an eye of a mammal.
15. The composition of claim 14 wherein the ocular dressing comprises a drug or combination of drugs for topical administration of the drug or drugs to the eye.
16. The ocular dressing of claim 14 , wherein the polymer is polyethylene glycol.
17. The ocular dressing of claim 14 , wherein the polyethylene glycol comprising the sulfhydryl, thiol, or mercaptan moiety forms disulfide bonds.
18. The ocular dressing of claim 14 wherein the polyethylene glycol is derivatized to contain a peptide comprising RGD.
19. The ocular dressing of claim 18 wherein a drug or drugs are conjugated to the peptide comprising RGD.
20. The ocular dressing of claim 18 wherein the peptide comprising RGD further comprises cysteine.
21. The ocular dressing of claim 18 wherein the peptide comprising RGD is a linear peptide having an amino acid sequence selected from the group consisting of Arg-Gly-Asp-Cys, Gly-Arg-Gly-Asp-Ser, and Gly-Arg-Gly-Asp-Ser-Pro.
22. The ocular dressing of claim 14 , wherein the cross-linker is selected from the group consisting of H2O2, a maleimide terminated polyethylene glycol, and a thiopyridine terminated polyethylene glycol.
23. The ocular dressing of claim 22 wherein the maleimide terminated polyethylene glycol is selected from the group consisting of bis-maleimido diethylene glycol and bis-maleimido triethylene glycol.
24. The ocular dressing of claim 22 wherein the thiopyridine terminated polyethylene glycol is obtained by reacting the 2-dithiopyridine or 4-dithiopyridine with thiol terminated polyethylene glycol.
25. The ocular dressing of claim 14 , wherein the composition further comprises an additive selected from the group consisting of polyvinyl pyrrolidone, polyethylene oxide, cellulose derivatives such as methyl cellulose, hydroxypropyl methylcellulose, hydroxypropyl cellulose, humectants such as glycerin, propylene glycol, polyethylene glycols having molecular weight in the range 100-10000, propylene glycol derivatives, and phospholipids.
26. The ocular of claim 14 wherein the composition provides controlled release of said drug.
27. A drug delivery device for delivering drug for treatment of the eye, said drug delivery device comprising a composition of claim 14 .
28. A method of treatment for promoting healing of a wound on the skin, said method comprising the steps of:
a. applying a reversibly cross-linked hydrogel composition of claim 1 to the wound, and
b. removing the hydrogel by dissolving said cross-links in the hydrogel composition.
29. A method of treatment for delivering a drug to a corneal surface of an eye, said method comprising the steps of:
a. providing a reversibly cross-linked hydrogel composition of claim 14 , and
b. removing the hydrogel by dissolving said cross-links in the hydrogel composition.
30. The method of claim 28 or 29 , wherein removal of the hydrogel dressing comprises reversing the cross-links using a reducing agent.
31. The method of claim 30 wherein the reducing agent is selected from the group consisting of cysteine and derivatives thereof, cysteine ethyl ester, cysteine methyl ester, gluthatione, cysteine hydrocholoride, dithiothretol, N-Ethylmalemide, phosphine derivatives tetrakis-hydroxymethyl phosphonium chloride and tris-diethylaminomethyl phosphine trialkylphosphine agents, such as Tris[2-carboxyethyl] phosphine and mercaptoethanols, 2,3-dimercapto-1-propanol, dinitrobenzoic acid, a thiol, a mercaptan, a sulfite or bisulfite or ammonium or sodium salts thereof, thioglycolic acid, thiolactic acid, cysteine, thioglycerol, thioglycolic hydrazide, thioglycolamide, glycerol monothioglycolate, beta-mercapto-propionic acid, N-hydroxyethyl mercapto-acetamide, N-methyl mercapto-acetamide, beta-mercapto-ethylamine, beta-mercapto-propionamide, 2-mercapto-ethanesulfonic acid, dimercapto-adipic acid, dithiothreitol, homocysteinethiolactone, and a polythiol derivative formed by the addition of cysteamine onto a maleic anhydride-alkylvinylether copolymer.
32. The method of claim 30 wherein the reducing agent is glutathione or cysteine.
33. The dressing of claim 4 or 15 comprising a drug selected from the group consisting of a topical anesthetic, antibiotic, antiseptic, analgesic, anti-inflammatory and a wound healing therapeutic agent.
34. The composition of claim 33 wherein the drug is lidocaine, benzocaine, butamben, dibucaine, oxybuprocaine, pramoxine, proparacaine, proxymetacaine, novocaine, procaine, tetracaine, doxycycline, minocycline, oxytetracycline, sancycline, dedimethylamino tetracycline, indomethacin, diclofenac, ibuprofen, naproxen, ketoprofen, dexamethasone, a vallinoid, olvanil, capsaicin, benzalkonium chloride, an antiglaucoma medication, pilocarpine, timolol, levobunolol, betaxolol, or carbacol.
35. The dressing of claim 1 or 14 wherein the dressing further comprises a growth factor.
36. The dressing of claim 35 wherein the growth factor is selected from the group consisting of a cytokine, epidermal growth factor (EGF), an EGF protein having one or more repeats of the conserved amino acid sequence: CX7CX4-5CX10-13CXCX8GXRC (where X represents any amino acid), transforming growth factor alpha (TGF-alpha), transforming growth factor beta (TGF-b), keratinocyte growth factor (KGF-2), fibroblast growth factor, fibronectin, fibrinogen, Granulocyte-Monocyte Colony Stimulating Factor (GM-CSF) and platelet-derived growth factor (PDGF).
37. A method of preparing a cross-linked hydrogel composition for application to the skin, said method comprising:
a. providing a polymer in solution,
b. providing in a separate solution a cross-linker that forms reversible cross-links,
c. administering both solutions concomitantly from at least one nozzle permitting mixing of the polymer and cross-linking solutions in order to provide rapid gelation of the reversibly cross-linked hydrogel at the site of administration.
38. The method of claim 37 wherein the rapid gelation occurs within less than 3 minutes.
39. The method of claim 37 wherein the solutions are administered as a spray.
40. The method of claim 37 wherein the method comprises administering the polymer and cross-linking solutions from a dual-barreled syringe.
41. The dressing of claim 1 or 14 wherein the hydrogel is bioadhesive.
42. The dressing of claim 1 or 14 wherein the ratio of polymer to cross-linker is in the range of about 5:1 to 1:5 w/w.
43. The dressing of claim 1 or 14 wherein polymer and cross-linker are provided as a solution having a concentration of less than 30% w/v.
44. A wound or ocular dressing kit, comprising
a first component comprising a hydrophilic polymer having a sulfhydryl, thiol, or mercaptan moiety;
a second component comprising a cross-linker capable of forming a reversible cross-linkages with the hydrophilic polymer,
said first and second components being supplied as separate solutions and being mixed together during administration to a wound site such that a reversible cross-linked gel matrix forms as a wound dressing on the surface of the wound in less than about 30 minutes.
45. The kit of claim 44 further comprising a drug, a growth factor, or both.
46. The kit of claim 44 further comprising a reducing agent for reversing the reversible cross-links of the formed hydrogel for dissolution and removal of the dressing from a wound.
Priority Applications (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US14/968,041 US20160199534A1 (en) | 2007-04-24 | 2015-12-14 | Dressing compositions and methods |
US15/168,542 US9763968B2 (en) | 2004-12-22 | 2016-05-31 | Hydrogel formulation for dermal and ocular delivery |
Applications Claiming Priority (4)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US92591007P | 2007-04-24 | 2007-04-24 | |
PCT/US2008/005246 WO2008133918A1 (en) | 2007-04-24 | 2008-04-23 | Dressing compositions and methods |
US45099510A | 2010-10-27 | 2010-10-27 | |
US14/968,041 US20160199534A1 (en) | 2007-04-24 | 2015-12-14 | Dressing compositions and methods |
Related Parent Applications (3)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/US2008/005246 Continuation WO2008133918A1 (en) | 2004-12-22 | 2008-04-23 | Dressing compositions and methods |
US12/450,995 Continuation US9211358B2 (en) | 2007-04-24 | 2008-04-23 | Dressing compositions and methods |
US15/168,542 Continuation US9763968B2 (en) | 2004-12-22 | 2016-05-31 | Hydrogel formulation for dermal and ocular delivery |
Related Child Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
US15/168,542 Continuation-In-Part US9763968B2 (en) | 2004-12-22 | 2016-05-31 | Hydrogel formulation for dermal and ocular delivery |
Publications (1)
Publication Number | Publication Date |
---|---|
US20160199534A1 true US20160199534A1 (en) | 2016-07-14 |
Family
ID=39925973
Family Applications (2)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
US12/450,995 Active 2032-04-13 US9211358B2 (en) | 2007-04-24 | 2008-04-23 | Dressing compositions and methods |
US14/968,041 Abandoned US20160199534A1 (en) | 2004-12-22 | 2015-12-14 | Dressing compositions and methods |
Family Applications Before (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
US12/450,995 Active 2032-04-13 US9211358B2 (en) | 2007-04-24 | 2008-04-23 | Dressing compositions and methods |
Country Status (2)
Country | Link |
---|---|
US (2) | US9211358B2 (en) |
WO (1) | WO2008133918A1 (en) |
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE102020211389A1 (en) | 2020-09-10 | 2022-03-10 | Beiersdorf Aktiengesellschaft | Wound closure preparation containing active ingredients |
WO2024022671A1 (en) * | 2022-07-26 | 2024-02-01 | Beiersdorf Ag | Method for protecting the skin when removing bonded skin coverings |
Families Citing this family (33)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2006069344A2 (en) | 2004-12-22 | 2006-06-29 | Rutgers, The State University Of New Jersey | Controlled release hydrogels |
GB0722820D0 (en) | 2007-11-21 | 2008-01-02 | Smith & Nephew | Vacuum assisted wound dressing |
HUE043133T2 (en) | 2007-11-21 | 2019-07-29 | Smith & Nephew | Wound dressing |
WO2009067711A2 (en) | 2007-11-21 | 2009-05-28 | T.J. Smith & Nephew, Limited | Suction device and dressing |
US11253399B2 (en) | 2007-12-06 | 2022-02-22 | Smith & Nephew Plc | Wound filling apparatuses and methods |
GB0723875D0 (en) | 2007-12-06 | 2008-01-16 | Smith & Nephew | Wound management |
US20130096518A1 (en) | 2007-12-06 | 2013-04-18 | Smith & Nephew Plc | Wound filling apparatuses and methods |
GB0803564D0 (en) | 2008-02-27 | 2008-04-02 | Smith & Nephew | Fluid collection |
JP2010024223A (en) * | 2008-06-16 | 2010-02-04 | Teikoku Seiyaku Co Ltd | Oxybuprocaine-containing analgesic/antipruritic preparation for external use |
US9061095B2 (en) | 2010-04-27 | 2015-06-23 | Smith & Nephew Plc | Wound dressing and method of use |
GB201011173D0 (en) | 2010-07-02 | 2010-08-18 | Smith & Nephew | Provision of wound filler |
US20140086975A1 (en) * | 2010-10-15 | 2014-03-27 | Rutgers, The State University Of New Jersey | Hydrogel formulation for dermal and ocular delivery |
GB201020005D0 (en) | 2010-11-25 | 2011-01-12 | Smith & Nephew | Composition 1-1 |
EP2643412B1 (en) | 2010-11-25 | 2016-08-17 | Smith & Nephew PLC | Composition i-ii and products and uses thereof |
US20150159066A1 (en) | 2011-11-25 | 2015-06-11 | Smith & Nephew Plc | Composition, apparatus, kit and method and uses thereof |
US20130149351A1 (en) * | 2011-12-09 | 2013-06-13 | University Of Southern California | Polymer scaffolds and their use in the treatment of vision loss |
EP3611205A1 (en) * | 2012-10-24 | 2020-02-19 | KCI Licensing, Inc. | Sulfhydryl-functionalized polymeric compositions for medical devices |
US9987391B2 (en) | 2013-03-13 | 2018-06-05 | St. Jude Medical Coordination Center Bvba | Injectable hydrogels |
US20160120706A1 (en) | 2013-03-15 | 2016-05-05 | Smith & Nephew Plc | Wound dressing sealant and use thereof |
US9993577B2 (en) | 2013-07-01 | 2018-06-12 | Trustees Of Boston University | Dissolvable hydrogel compositions for wound management and methods of use |
US9579257B2 (en) | 2013-08-20 | 2017-02-28 | Anutra Medical, Inc. | Haptic feedback and audible output syringe |
USD763433S1 (en) | 2014-06-06 | 2016-08-09 | Anutra Medical, Inc. | Delivery system cassette |
USD774182S1 (en) | 2014-06-06 | 2016-12-13 | Anutra Medical, Inc. | Anesthetic delivery device |
USD750768S1 (en) | 2014-06-06 | 2016-03-01 | Anutra Medical, Inc. | Fluid administration syringe |
EP3250163B1 (en) | 2015-01-27 | 2023-07-12 | Medivance Incorporated | Medical pad for thermotherapy |
US11896724B2 (en) * | 2019-10-07 | 2024-02-13 | Arizona Board Of Regents On Behalf Of Arizona State University | Bioactive polymeric dressing for accelerated wound closure |
CN115038465A (en) * | 2019-12-06 | 2022-09-09 | 南洋理工大学 | Inflammation-reactive anti-inflammatory hydrogel |
WO2021140465A1 (en) * | 2020-01-07 | 2021-07-15 | Kci Licensing, Inc. | Wound dressings with acid-induced growth factor release |
CN111234263A (en) * | 2020-01-14 | 2020-06-05 | 浙江大学 | Preparation method of injectable polyethylene glycol active hydrogel |
DE102021200975B4 (en) * | 2021-02-03 | 2022-11-24 | Beiersdorf Aktiengesellschaft | Biologically-based wound closure preparation |
CN113265050B (en) * | 2021-04-30 | 2022-08-23 | 浙江大学 | Degradable high polymer material, self-assembled nano composite and application |
CN115177783B (en) * | 2022-06-27 | 2024-01-23 | 中国人民解放军空军军医大学 | Double-load intelligent hydrogel |
CN115645626B (en) * | 2022-09-07 | 2024-01-09 | 青岛科技大学 | High-strength antibacterial purslane hydrogel and preparation method thereof |
Citations (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20020076443A1 (en) * | 2000-06-19 | 2002-06-20 | Stanley Stein | Multiple phase cross-linked compositions and uses thereof |
US20030012818A1 (en) * | 2001-04-25 | 2003-01-16 | Eidgenossische Technische Hochschule Zurich And Universitat Zurich | Drug delivery matrices to enhance wound healing |
US20030166833A1 (en) * | 2001-12-18 | 2003-09-04 | Eidgenossische Technische Hochschule Zurich | Growth factor modified protein matrices for tissue engineering |
US6818018B1 (en) * | 1998-08-14 | 2004-11-16 | Incept Llc | In situ polymerizable hydrogels |
US20050256030A1 (en) * | 2004-02-23 | 2005-11-17 | Bainian Feng | Heterocyclic self-immolative linkers and conjugates |
US20080253987A1 (en) * | 2007-04-13 | 2008-10-16 | Kuros Biosurgery Ag | Polymeric tissue sealant |
Family Cites Families (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6565842B1 (en) * | 1995-06-07 | 2003-05-20 | American Bioscience, Inc. | Crosslinkable polypeptide compositions |
US6514535B2 (en) * | 1999-05-21 | 2003-02-04 | Noveon Ip Holdings Corp. | Bioadhesive hydrogels with functionalized degradable crosslinks |
AU6901000A (en) * | 1999-08-10 | 2001-03-05 | Acorda Therapeutics, Inc. | Bioartificial device for propagation of tissue, preparation and uses thereof |
US7811480B2 (en) * | 2004-03-04 | 2010-10-12 | Transitions Optical, Inc. | Photochromic optical article |
-
2008
- 2008-04-23 WO PCT/US2008/005246 patent/WO2008133918A1/en active Application Filing
- 2008-04-23 US US12/450,995 patent/US9211358B2/en active Active
-
2015
- 2015-12-14 US US14/968,041 patent/US20160199534A1/en not_active Abandoned
Patent Citations (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6818018B1 (en) * | 1998-08-14 | 2004-11-16 | Incept Llc | In situ polymerizable hydrogels |
US20020076443A1 (en) * | 2000-06-19 | 2002-06-20 | Stanley Stein | Multiple phase cross-linked compositions and uses thereof |
US20030012818A1 (en) * | 2001-04-25 | 2003-01-16 | Eidgenossische Technische Hochschule Zurich And Universitat Zurich | Drug delivery matrices to enhance wound healing |
US20030166833A1 (en) * | 2001-12-18 | 2003-09-04 | Eidgenossische Technische Hochschule Zurich | Growth factor modified protein matrices for tissue engineering |
US20050256030A1 (en) * | 2004-02-23 | 2005-11-17 | Bainian Feng | Heterocyclic self-immolative linkers and conjugates |
US20080253987A1 (en) * | 2007-04-13 | 2008-10-16 | Kuros Biosurgery Ag | Polymeric tissue sealant |
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE102020211389A1 (en) | 2020-09-10 | 2022-03-10 | Beiersdorf Aktiengesellschaft | Wound closure preparation containing active ingredients |
WO2024022671A1 (en) * | 2022-07-26 | 2024-02-01 | Beiersdorf Ag | Method for protecting the skin when removing bonded skin coverings |
Also Published As
Publication number | Publication date |
---|---|
WO2008133918A1 (en) | 2008-11-06 |
US9211358B2 (en) | 2015-12-15 |
US20110033503A1 (en) | 2011-02-10 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
US9211358B2 (en) | Dressing compositions and methods | |
ES2526700T3 (en) | Adhesive composition | |
Valenta et al. | The use of polymers for dermal and transdermal delivery | |
ES2272586T3 (en) | PREPARATION WITH PHARMACEUTICAL VEHICLE APPLICABLE TO MUCOSE SURFACES. | |
ES2381639T3 (en) | Polymeric fabric sealant | |
JP5523114B2 (en) | Coating formulations that prevent and reduce scarring | |
AU2008342920B2 (en) | Topical application and formulation of erythropoietin for skin wound healing | |
AU2014229273B2 (en) | Materials for gastrointestinal obstruction prevention | |
JP2009508991A (en) | Polymer composition and methods for making and using the same | |
EP2867298A1 (en) | Biocompatible polyacrylate compositions and methods of use | |
JP5646507B2 (en) | Hyaluronic acid-containing composition for treating wounds, scars, and post-surgical adhesion formation | |
US5980883A (en) | Polymer gel for medical use | |
US20160338969A1 (en) | Biocompatible polyacrylate compositions and methods of use | |
JP2022023110A (en) | Bimodal treatment methods and compositions for gastrointestinal lesions with active bleeding | |
CA2425206A1 (en) | Treatment of trauma, and other indications | |
ES2305897T3 (en) | SURFACE PROTECTION OF EXPOSED BIOLOGICAL FABRICS. | |
US20030007951A1 (en) | Treatment of trauma | |
RU2578969C1 (en) | Agent for treating damage to external body tissues (versions) and method of producing same | |
CN115403760A (en) | Amino acid modified polymer for preventing sticking and application thereof | |
CN116063584A (en) | Chitosan-gallic acid copolymer and application thereof in preparation of hydrogel and wound repair | |
KR100483227B1 (en) | Fushidine acid external spray composition | |
EA001077B1 (en) | Use of component b as cicatrizant comprising its pharmaceutical composition and method of treatment | |
JP2002527458A (en) | Method and composition for forming a protective film and / or medicinal film in situ on body tissue | |
MXPA00003491A (en) | Method and compositions for in situ |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
STPP | Information on status: patent application and granting procedure in general |
Free format text: RESPONSE TO NON-FINAL OFFICE ACTION ENTERED AND FORWARDED TO EXAMINER |
|
STPP | Information on status: patent application and granting procedure in general |
Free format text: NON FINAL ACTION MAILED |
|
STCB | Information on status: application discontinuation |
Free format text: ABANDONED -- FAILURE TO RESPOND TO AN OFFICE ACTION |
|
AS | Assignment |
Owner name: NIH - DEITR, MARYLAND Free format text: CONFIRMATORY LICENSE;ASSIGNOR:RUTGERS, THE STATE UNIVERSITY OF N.J.;REEL/FRAME:056044/0604 Effective date: 20210422 |