US20120302626A1 - Microrna and use thereof in identification of b cell malignancies - Google Patents

Abstract

Disclosed are nucleic acid sequences, including microRNA sequences and cDNA sequences, as well as vectors, DNA libraries, microarrays, and recombinant cells comprising the nucleic acid sequences described herein. Methods of determining the B cell stage from which a B cell malignancy is derived. Methods of identifying B cell malignancies are also provided. Methods of diagnosing B cell malignancies are provided. Such methods comprise, in certain embodiments, detecting one or more microRNAs or cDNAs as disclosed herein.

Description

CROSS REFERENCE TO RELATED APPLICATIONS
• This application is related to and claims the benefit of priority of U.S. Provisional Application Ser. No. 61/266,733, filed Dec. 4, 2009, which is incorporated herein by, reference.
STATEMENT OF GOVERNMENT SUPPORT
• This invention was made with United States government support from the National Institutes of Health grant no. K12-CA-100639. The United States government has certain rights in this invention.
SEQUENCE LISTING
• The sequence listing is filed with the application in electronic format only and is incorporated by reference herein. The sequence listing text file “WO_ASFILED_SequenceListing.txt” was created on Dec. 3, 2010, and is 262,254 bytes in size.
BACKGROUND
• Naive B cells migrate through the circulation to lymphoid organs where they undergo the T cell-dependent germinal center reaction. Adaptive immunity is acquired as specific antigen-reactive germinal center B cells differentiate into the major effector B cells of the adaptive immune system: memory cells and plasma cells. See, e.g., FIG. 1A. Although the role of specific transcription factors in mature B cell differentiation has been examined (see Nutt et al. Nature. 1999; 401:556-562; Chang et al. Proc Natl Acad Sci USA. 1996; 93:6947-6952; Turner et al. Cell. 1994; 77:297-306; Shaffer et al., Immunity. 2004; 21:81-93; and Schebesta et al. Curr Opin Immunol. 2002; 14:216-223), mechanisms regulating such transcription factors during mature B cell differentiation are largely unknown.
• Many malignancies derived from mature B cells are known and are believed to constitute the majority of leukemias and lymphomas. Such malignancies appear to reflect defined stages of normal B cell differentiation. Diagnosis of leukemias and lymphomas can be particularly difficult because of their shared lineage. These cancers frequently display overlapping morphologies, genetic abnormalities, and expression of surface markers, which can complicate the diagnosis. However, the distinction of these tumors is clinically important because there are important differences in the treatments and expected response to treatment. Thus methods that improve the accuracy of their diagnosis should provide to improved outcomes for these patients.
• MicroRNAs are commonly 18-22 nucleotide-long RNA molecules that regulate expression of genes. There is an increasing recognition of the role of microRNAs in oncogenesis, lineage-selection, and immune cell function, including early B cell differentiation. See Calin et al. N Engl J Med. 2005; 353:1793-1801; O'Donnell et al. Nature. 2005; 435:839-843; Chen et al. Science. 2004; 303:83-86; Lim et al. Nature. 2005; 433:769-773; Li et al. Cell. 2007; 129:147-161; Xiao et al., Nat Immunol. 2008; 9:405-414; Baltimore et al. Nat Immunol. 2008; 9:839-845; and Ventura et al. Cell. 2008; 132:875-886. However, the full extent and function of microRNA expression during mature B cell differentiation and in B cell malignancies are not known.
• Correct diagnosis of B cell malignancies is important from both a clinical standpoint and from the standpoint of setting appropriate patient expectations. A misdiagnosed B cell malignancy may lead to an inappropriate therapy, which can unnecessarily endanger the patient's life and/or be an ineffective treatment for the B cell malignancy. As an example, the diagnostic distinction of Burkitt lymphoma (BL) from diffuse large B cell lymphomas (DLBCLs) can be difficult because of overlapping morphology, immunophenotype and cytogenetics. Burkitt lymphoma tumors are molecularly distinct from DLBCL, however. The difficulty and importance of obtaining the correct diagnosis in BL was highlighted by the experience of a multicenter clinical trial, CALGB trial#925119, in which nearly half of the 100 patients with an assigned diagnosis of BL were found to have another diagnosis upon further pathology review.
• If diagnosed and treated appropriately, nearly 80% of patients with BL can be cured with intensive (high dose) chemotherapy regimens. Thus, a misclassification of BL as DLBCL can result in a missed opportunity to cure the malignancy. On the other hand, misclassification of DLBCL as BL leads to unnecessarily morbidity from intensive chemotherapy regimens. Thus, methods that improve the diagnosis of BL, and other B cell malignancies, can provide better outcomes in patients.
• DLBCLs can also be subclassified into two different B cell malignancies, activated B-cell (ABC) DLBCL and germinal center B cell like (GCB) DLBCL. There are at least two important clinical applications for the molecular sub-grouping of DLBCL patients. First, the prognostic information could inform the choices and expectations of patients and their physicians. Second, the important molecular differences in these subgroups form the basis of testing different targeted therapies in these patients. The possibility of a differential response to therapy among ABC and GCB DLBCLs is supported by data that suggest that the benefit of receiving a proteosome inhibitor, bortozemib, is predominantly limited to those patients who have ABC DLBCL. However, the clinical distinction of the subgroups of DLBCL using immunohistochemistry is difficult with current methods distinguishing GCB DLBCL from non-GCB DLBCL with limited success.
• Mature B cell differentiation is important for the development of adaptive immunity. The process is also of interest because B cell malignancies are common and retain a number of features derived from their normal counterpart B cell subsets. Unlike other maturation pathways in the hematopoietic and other cell lineages, successive stages of mature B cells do not simply signify progressive differentiation away from the stem cell stage. Rather, each stage represents a specialized state with specific functions. Thus, germinal center (GC) cells interact with CD4 T cells and dendritic cells and undergo somatic hypermutation and Ig-heavy chain class-switching. On the other hand, plasma cells secrete immunoglobulin, while memory cells are primed to proliferate and differentiate into plasma cells upon repeat contact with antigen. The specialized functions demand a finely tuned program of gene regulation.
• MicroRNAs represent a novel class of biomarkers that provide new opportunities for clinical translation. First, intact microRNAs can be isolated from tissues preserved using standard methods, such as formalin fixed, paraffin embedded (FFPE) tissue. Thus, microRNA-based biomarkers could be easy to translate to clinical use. Second, microRNAs can be readily assayed using real-time PCR and other methods available in conventional pathology.
SUMMARY
• In an aspect the disclosure provides an isolated nucleic acid molecule having at least 80% sequence identity to any one of SEQ ID NOs: 763-1350 or 1565 or a complementary sequence thereof. Embodiments provide for sequence identity of at least 90% or 95%.
• In an aspect the disclosure provides an isolated nucleic acid molecule comprises any one of SEQ ID NOs: 763-1350 or 1565 or a complementary sequence thereof. Embodiments provide for isolated nucleic acid molecules comprising a primary miRNA, a precursor miRNA, a mature miRNA, or a DNA molecule coding therefore. Embodiments further provide for a cDNA molecule comprising sequence that corresponds to a miRNA sequence of any one of SEQ ID NOs: 763-1350 or 1565.
• Aspects of the disclosure provide compositions, pharmaceutical compositions, vectors, host cells, and DNA libraries comprising at least one nucleic acid molecule described herein.
• In an aspect the disclosure provides a method of determining the B cell stage of a B cell malignancy in a subject comprising determining the level of expression of at least one microRNA in a sample comprising a B cell taken from the subject relative the level of expression of the at least one microRNA in a control sample, wherein the at least one microRNA is selected from the microRNAs listed in Table 4.
• In an aspect the disclosure provides a method of identifying a B cell malignancy in a subject comprising determining the level of expression of at least one microRNA in a sample comprising a B cell taken from the subject relative the level of expression of the at least one microRNA in a control sample, wherein the at least one microRNA is selected from the microRNAs listed in Table 4.
• In an aspect the disclosure provides a method of diagnosing a B cell malignancy in a subject comprising determining the level of expression of at least one microRNA in a sample comprising a B cell taken from the subject relative the level of expression of the at least one microRNA in a control sample, wherein the at least one microRNA is selected from the microRNAs listed in Table 4.
• In an aspect the disclosure provides a method of identifying a B cell malignancy in a subject comprising determining the level of expression of at least one microRNA in a sample comprising a B cell taken from the subject relative the level of expression of the at least one microRNA in a control sample, wherein the at least one microRNA is selected from the microRNAs listed in any one of Tables 7 to 35.
• In an aspect the disclosure provides a method of diagnosing a B cell malignancy in a subject comprising determining the level of expression of at least one microRNA in a sample comprising a B cell taken from the subject relative to the level of expression of the at least one microRNA in a control sample, wherein the at least one microRNA is selected from the microRNAs listed in any one of Tables 7 to 35.
• Embodiments of these aspects provide for identification or diagnosis of a B cell malignancy selected from chronic lymphocytic leukemia, follicular lymphoma, Hodgkin's lymphoma, activated B-cell diffuse large B cell lymphoma (DLBCL), germinal center-like DLBCL, and Burkitt lymphoma.
• In an aspect the disclosure provides a method of determining whether a B cell malignancy in a sample is Burkitt lymphoma, activated B cell-like diffuse large B cell lymphoma (DLBCL), or germinal center-like DLBCL, comprising determining the level of expression of at least one microRNA in the sample relative to the level of expression of the at least one microRNA in a control sample, wherein the at least one microRNA is selected from the microRNAs listed in Table 10, column “BL miRNA list”; Table 14, column “BL High”; Table 11; Table 14, column “ABC High”; Table 10, column “GCB miRNA list”; Table 14, column “GCB High”; Table 32, or Table 35.
• In an aspect the disclosure provides a method of determining whether a B cell malignancy in a sample is Burkitt lymphoma, activated B cell-like diffuse large B cell lymphoma (DLBCL), or germinal center-like DLBCL, comprising determining the level of expression of at least one microRNA in the sample relative to the level of expression of the at least one microRNA in a control sample, wherein the at least one microRNA is selected from the microRNAs listed in at least one column labeled “GCBvsBL” or “GCBvsABC” in Table 7 or “ABCvsBL” in Table 8.
• In an aspect the disclosure provides a method of determining whether a B cell malignancy in a sample is activated B cell-like diffuse large B cell lymphoma (DLBCL), or germinal center-like DLBCL, comprising determining the level of expression of at least one microRNA in the sample relative to the level of expression of the at least one microRNA in a control sample, wherein the at least one microRNA is selected from the microRNAs listed in Table 35.
• In an aspect the disclosure provides a microarray comprising miRNA-specific probe oligonucleotides wherein at least one miRNA-specific probe oligonucleotide is specific for a sequence of SEQ ID NOs: 763-1350 or 1565, or any combination thereof.
• In an aspect the disclosure provides a kit comprising at least one primer sequence that can detect any one of SEQ ID NOs: 763-1350 or 1565, or a combination thereof.
• In an aspect the disclosure provides a kit comprising at least one isolated nucleic acid molecule having a sequence of any one of SEQ ID NOs: 1351-1564.
• In an aspect, the disclosure provides a method of identifying a B cell malignancy comprising determining the level of expression of at least one microRNA selected from the microRNAs listed in Table 4. In certain embodiments, a method comprises determining the level of expression of at least one microRNA selected from the microRNAs listed in Tables 7-35.
• Other aspects and embodiments will be apparent to one of skill in the art in light of the following detailed description.
DESCRIPTION OF THE FIGURES
• FIG. 1 shows that mature B cell subsets demonstrate distinct microRNA profiles. FIG. 1A is a diagram showing the overall schema of mature B cell differentiation. FIG. 1B shows selection of B cell subsets using flow cytometry. FIG. 1C shows the distinction between naive and memory B cells based on IgD and CD27 expression using flow cytometry. FIG. 1D shows relative expression of microRNA in the naive to germinal center B cell transition. FIG. 1E shows relative expression of mRNA in the naive to germinal center B cell transition. FIG. 1F shows relative expression of microRNA in the germinal center B cell to plasma cell transition. FIG. 1G shows relative expression of mRNA in the germinal center B cell to plasma cell transition. FIG. 1H shows relative expression of microRNA in the germinal center B cell to memory B cell transition. FIG. 1I shows relative expression of mRNA in the germinal center B cell to memory B cell transition. In FIGS. 1D, 1F, and 1H, miRNAs that were, on average, at least 2-fold differentially expressed at a false discovery rate of less than 5% are shown according to the color scale. In Figures and 1E, 1G, and 1I, mRNAs that were, on average, at least 2-fold differentially expressed at a false discovery rate of less than 1% are shown according to the color scale. FIG. 1J shows that expression of certain microRNA processing genes, DGCR8, DICER1, EIF2C2, DROSHA, and XP05 is unchanged among the B cell subsets (P>0.1 in all cases).
• FIG. 2 shows experimental validation of the interaction of miR-223, which is expressed highly in naive and memory B cells compared to germinal center B cells, and targets the transcription factor LM02. FIG. 2A shows base-pairing of the 3′UTR of the LM02 gene with nucleotides 1-8 of miR-223. This 8-mer is highly conserved across a number of species and serves as a potential binding site for miR-223. FIG. 2B shows the effects of over-expression of miR-223 in germinal center lymphoma-derived BJAB cells in 3 separate experiments. FIG. 2C shows the relative LM02 protein expression from a representative experiment (from 3 replicates) transfecting a scrambled control versus a precursor for miR-223 in BJAB cells. FIG. 2D shows average expression of LM02 relative to Actin over three Western blots of BJAB cells transfected with a scrambled control versus a precursor for miR-223. FIG. 2E shows luciferase activity in BJAB cells transfected with a vector comprising either a luciferase gene coupled to the 3 ′UTR of the LM02 gene or a luciferase gene coupled to the 3 ′UTR of the LM02 gene with the miR-223 binding site mutated, and cotransfected with miR-223.
• FIG. 3 shows experimental validation of the interaction of miR-9 and miR-30, which are expressed highly in germinal center B cells compared to plasma cells and target the transcription factor PRDM1. FIG. 3A shows base-pairing of the 3′UTR of the PRDM1 gene with the 5′ seed region of miR-9 and the miR-30 family. FIG. 3B shows the effects of over-expressing miR-9 and 2 members of the miR-30 family, miR-30b and miR-30d, in plasma cell myeloma-derived U266 cells in 3 separate experiments. FIG. 3C shows the relative PRDM1 protein expression from a representative experiment (from 3 replicates) transfecting a scrambled control versus a precursor for miR-9, miR-30b, or miR-30d in U266 cells. FIG. 3D shows the average expression of PRDM1 relative to Actin over three Western blots of U266 cells transfected with a scrambled control versus a precursor for miR-9, miR-30b, or miR-30d. (P<0.05 for miR-30b and miR-30d, P=0.08 for miR-9.) FIG. 3E shows repression of luciferase activity from the PRDM1 3′UTR construct by overexpression of miR-9, miR-30b, and miR-30d wild-type and mutant sequences.
• FIG. 4 shows that expression of microRNAs in normal B cells is conserved in certain B cell malignancies. FIG. 4A shows lineage prediction of both IgV mutated and unmutated chronic lymphocytic leukemia, germinal center B cell derived DLBCL, and Burkitt lymphoma based on differential expression of microRNAs in normal naïve B cells and germinal center B cells (microRNAs depicted in FIG. 1D). FIG. 4B shows miRNAs that were found to be differentially expressed (P<0.05) in malignant cells and normal cells as discussed in Example 5. FIG. 4C shows cloning frequency of miRNAs in unselected mature B cells (N=3) and certain B cell malignancies (N=42) from a previously published study (“sequencing data”), as discussed in Example 5. FIG. 4D shows differentially expressed miRNAs that distinguish Burkitt lymphoma, activated B cell-like (ABC) diffuse large B cell lymphoma (DLBCL), germinal center-like DLBCL (GCB DLBCL), and chronic lymphocytic leukemia. Predictor miRNAs from each pair-wise comparison that distinguish each entity are shown in the boxes.
• FIG. 5 shows the distribution of miRNAs present in B-cell subsets.
• FIG. 6 shows that certain microRNA targets are expressed at lower levels. FIG. 6A shows density plots of the expression frequency of predicted mRNA targets of miRNAs expressed highly in germinal center B cells compared to naive cells. FIG. 6B shows density plots of the expression frequency of predicted mRNA targets of miRNAs expressed highly in germinal center B cells compared to plasma cells. FIG. 6C shows density plots of the expression frequency of predicted mRNA targets of miRNAs expressed highly in the germinal center B cells compared to memory B cells.
• FIG. 7 shows that differentially expressed transcription factors are frequently microRNA targets. In FIG. 7A, the left panel indicates the proportion of transcription factors that are differentially expressed in the naive to germinal center B-cell transition that are also predicted targets of differentially expressed miRNAs. The right panel indicates the proportion of transcription factors that are not differentially expressed and also are predicted targets of differentially expressed miRNA in that stage-transition. The p-value indicates the results of a chi-squared test for the enrichment of predicted miRNA targets among the differentially expressed transcription factors. FIG. 7B shows a similar analysis of the germinal center to plasma cell transition. FIG. 7C shows a similar analysis of the germinal center to memory cell transition.
• FIG. 8 shows that Mybl transcript levels decrease with miR-223 overexpression. FIG. 8A shows base-pairing of the 3′UTR of the MYBL1 gene with nucleotides 2-8 of miR-223. This 7-mer is highly conserved across a number of species and serves as a potential binding site for miR-223. FIG. 8B shows the effects of over-expression of miR-223 in germinal center lymphoma-derived BJAB cells in 3 separate experiments.
• FIG. 9 shows the specificity of real-time PCR probes for members of the miR-30 family.
• FIG. 10 shows the results of leave one out cross validation applied to the predictors for Burkitt lymphoma, chronic lymphocytic leukemia, activated B-cell diffuse large B-cell lymphoma, and germinal center B-cell DLBCL.
• FIG. 11 shows differentially expressed miRNAs that distinguish activated B cell-like diffuse large B cell lymphoma (DLBCL), germinal center-like DLBCL (GCB DLBCL), Burkitt lymphoma, chronic lymphocytic leukemia, follicular lymphoma, and Hodgkin's lymphoma. Predictor miRNAs from each pair-wise comparison that distinguish each entity are shown in the boxes.
DETAILED DESCRIPTION
• All patent and non-patent literature references that are cited herein are incorporated herein by reference in their entirety.
• In a general sense, the disclosure relates to nucleic acid sequences, such as microRNAs (miRNA), as well as to the identification and analysis of microRNA expression levels and/or patterns in B cells. Through concomitant microRNA and mRNA profiling, the inventors have identified regulatory roles for microRNAs at each stage in mature B cell differentiation. This provides methods identifying microRNA-mediated regulation of oncogenes and key transcription factors in B cell differentiation. This work establishes the landscape of normal microRNA expression in mature B cells and its role in regulating normal B cell differentiation. Further, our work demonstrates that in contrast to the described down-regulation in other malignancies, stage-specific microRNAs are retained in B cell malignancies. The lineage of common B cell malignancies can be predicted based upon miRNA profiles of normal B cells, pointing to a role for microRNAs in the maintenance of mature B cell phenotypes in normal and malignant B cells.
• In an aspect, the disclosure relates to an isolated nucleic acid molecule comprising: (a) a nucleotide sequence as shown in Table 32; (b) a nucleotide sequence which is the complement of (a), (c) a nucleotide sequence comprising a sequence identity of at least 80%, (e.g., 80, 81, 82, 83, 84, 85, 86, 87, 88, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, or at least 99%), to a sequence of (a) or (b) and/or (d) a nucleotide sequence which hybridizes under stringent conditions to a sequence of (a), (b) and/or (c). In some embodiments, the identity of sequence (c) to a sequence of (a) or (b) is at least 90%. In other embodiments, the identity of sequence (c) to a sequence of (a) or (b) at least 95%. The percent identity can be calculated by any routine method used by one of skill in the art such as, for example, the methods described herein.
• In embodiments, the isolated nucleic acid molecule relates to a miRNA molecule and analogs thereof, a miRNA precursor molecule, or a primary miRNA molecule, as well as to DNA molecules encoding miRNA, miRNA precursor, or primary miRNA molecules. Accordingly, in such embodiments, the isolated nucleic acid molecule can function as a miRNA molecule under suitable conditions. Suitable conditions include, but are not limited to, various buffer systems that approximate physiologically relevant ionic concentrations and pHs, as well as physiological conditions.
• In some embodiments, the nucleic acid molecule comprises a sequence that hybridizes to a nucleotide sequence as shown in Table 32, a complementary sequence thereof or a nucleic acid molecule having at least 80% sequence identity under stringent hybridization conditions. The basic parameters affecting the choice of hybridization conditions and guidance for devising suitable conditions are set forth by Sambrook, et al. (See, 1989, Molecular Cloning: A Laboratory Manual, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y.; and Current Protocols in Molecular Biology, 1995, Ausubel et al., eds., John Wiley & Sons, Inc.), and can be readily determined by those of ordinary skill in the art based on, for example, the length and/or base composition of the DNA. Generally, stringent salt concentration will ordinarily be less than about 750 mM NaCl and 75 mM trisodium citrate, or less than about 500 mM NaCl and 50 mM trisodium citrate, or even less than about 250 mM NaCl and 25 mM trisodium citrate. High stringency hybridization conditions can be obtained by adding an amount of organic solvent (e.g., at least about 35% to about 50% formamide). Stringent temperature conditions will ordinarily include temperatures of at least about 30° C., (e.g., at least about 37° C., 42° C., 45° C., 50° C., or 55° C.). Varying additional parameters, such as hybridization time, the concentration of detergent (e.g., 0.1-1.0% sodium dodecyl sulfate (SDS)), and the inclusion or exclusion of carrier DNA (e.g., about 100-200 μg/ml denatured salmon sperm DNA (ssDNA)), are well known to those skilled in the art. Stringent hybridization conditions are known in the art and include non-limiting examples such as, washing for 1 hr in 300 mM NaCl, 30 mM trisodium citrate and 0.1% SDS at 45-50° C.; washing for 1 h in 300 mM NaCl, 30 mM trisodium citrate and 0.1% SDS at 45-50° C.; in 500 mM NaCl, 50 mM trisodium citrate, 1% SDS, 35% formamide, and 100 μg/ml denatured salmon sperm DNA (ssDNA) at 37° C.; or in 250 mM NaCl, 25 mM trisodium citrate, 1% SDS, 50% formamide, and 200 μg/ml ssDNA at 42° C. Useful variations on these conditions will be readily apparent to those skilled in the art.
• The isolated nucleic acid molecules provided herein suitably have a length of from about 18 to about 100 nucleotides. In embodiments wherein the isolated nucleic acid molecules are miRNAs, the lengths of the miRNAs are suitably in an expected range for the particular type of miRNA molecule. For example, mature miRNAs are typically from about 15 to about 28 nucleotides in length, and suitably have a length of about 19 to about 24 nucleotides (e.g., 19, 20, 21, 22, 23, or 24 nucleotides). Precursor miRNAs typically comprise the mature miRNA sequence and contain a stem-loop structure, suitably of length of about 50 to about 90 nucleotides (e.g., 50, 55, 60, 65, 70, 75, 80, 85, or 90 nucleotides). Primary miRNAs (e.g., a primary transcript comprising a precursor miRNA) can suitably have a length of greater than 100 nucleotides.
• The nucleic acid molecules can be provided in either a single-stranded or double-stranded form. Typically, a miRNA as such is identified as a single-stranded molecule, while the precursor miRNA is typically at least partially self-complementary and capable of forming double-stranded portions, e.g. stem- and loop-structures. DNA molecules encoding the miRNA and miRNA precursor molecules (e.g., expression vectors, cloning vectors, and the like) are typically double-stranded. The individual nucleic acids that comprise the isolated nucleic acid molecules can be selected from RNA, DNA, or nucleic acid analog molecules, such as chemically modified sugar (e.g., 2′-modified (2′-F, 2′-OMe, etc.) or backbone (e.g., phosphorothioates), or cap (e.g., 5′- and/or 3′-abasic groups) moieties of ribonucleotides or deoxyribonucleotides. Other nucleic acid analogs, such as peptide nucleic acids (PNA) or locked nucleic acids (LNA), are also suitable in various embodiments described herein. In some embodiments the nucleic acid molecules can comprise any combination of nucleic acid analog(s).
• While many of the nucleic acid molecules in the Tables are identified as RNA sequences, e.g., miRNAs, the disclosure of those sequences should be understood to encompass the corresponding DNA (e.g., cDNA) sequences, wherein the uracil (U) nucleotides of the disclosed RNAs are substituted by thymidine (T) nucleotides in the corresponding DNA. One of skill in the art is able to generate such DNA sequences (e.g., cDNA) through routine microbiological techniques known in the art such as, for example, reverse transcription using methods that incorporate the reverse transcriptase enzyme.
• In another aspect, the disclosure provides a recombinant expression vector comprising a recombinant nucleic acid sequence operatively linked to an expression control sequence, wherein expression of the recombinant nucleic acid sequence provides a miRNA sequence, a precursor miRNA sequence, or a primary miRNA sequence as described herein. The resulting sequence (e.g., primary or precursor miRNAs) can optionally be further processed to provide the miRNA sequence. In embodiments, the recombinant expression vector comprises at least one sequence in Table 32. Any suitable expression vector can be used such as, for example, a DNA vector (e.g., viral vector, plasmid, etc.). In some embodiments the expression vector is selected for expression in a eukaryotic cell such as, for example, a mammalian cell. One of skill in the art will be able to select an appropriate vector based on the particular application and/or expression system to be employed.
• Thus, embodiments provide nucleic acid constructs in the form of plasmids, vectors, transcription or expression cassettes which comprise at least one nucleotide sequence encoding a miRNA described herein, or fragments thereof, and a suitable promoter region. Suitable vectors can be chosen or constructed, which contain appropriate regulatory sequences, such as promoter sequences, terminator sequences, polyadenylation sequences, enhancer sequences, marker genes and other sequences as desired. Vectors can be plasmids, phage (e.g. phage, or phagemid) or viral (e.g. lentivirus, adenovirus, AAV) or any other appropriate vector. For further details see, for example, Molecular Cloning: a Laboratory Manual: 2nd edition, Sambrook et al., 1989, Cold Spring Harbor Laboratory Press.
• Relatedness of Nucleic Acid Molecules/Sequences
• The term “identity” refers to a relationship between the sequences of two or more two or more nucleic acid molecules, as determined by comparing the sequences. In the art, “identity” also means the degree of sequence relatedness between amino acid or nucleic acid molecule sequences, as the case may be, as determined by the match between strings of nucleotide or amino acid sequences. “Identity” measures the percent of identical matches between two or more sequences with gap alignments addressed by a particular mathematical model or computer programs (i.e., “algorithms”).
• Identity of related nucleic acid molecules can be readily calculated by known methods, including but not limited to those described in Computational Molecular Biology, Lesk, A. M., ed., Oxford University Press, New York, 1988; Biocomputing: Informatics and Genome Projects, Smith, D. W., ed., Academic Press, New York, 19933; Computer Analysis of Sequence Data, Part 1, Griffin, A. M., and Griffin, H. G., eds., Humana Press, New Jersey, 1994; Sequence Analysis in Molecular Biology, von Heinje, G., Academic Press, 1987; and Sequence Analysis Primer, Gribskov, M. and Devereux, J., eds., M. Stockton Press, New York, 1991; and Carillo, H., and Lipman, D., SIAM J. Applied Math., 48:1073 (1988).
• Non-limiting methods for determining identity are designed to give the largest match between the sequences tested. Methods to determine identity are codified in publicly available computer programs. Preferred computer program methods to determine identity between two sequences include, but are not limited to, the GCG program package, including GAP (Devereux, et al., Nucleic Acids Research 12:387 [1984]; Genetics Computer Group, University of Wisconsin, Madison, Wis.), BLASTN, and FASTA (Atschul et al., J. Molec. Biol. 215:403-410 [1990]). The BLAST X program is publicly available from the National Center for Biotechnology Information (NCBI) and other sources (BLAST Manual, Altschul] et al., NCB NLM NIH Bethesda, Md. 20894; Altschul et al., J. Mol. Biol. 215:403-410 [1990]). The well known Smith Waterman algorithm may also be used to determine identity.
• Exemplary parameters for nucleic acid molecule sequence comparison include the following:
• Algorithm: Needleman and Wunsch, J. Mol Biol. 48:443-453 (1970)
• Comparison matrix: matches=+10, mismatch=0
• Gap Penalty: 50
• Gap Length Penalty: 3
• The GAP program is also useful with the above parameters. The aforementioned parameters are the default parameters for nucleic acid molecule comparisons.
• Other exemplary algorithms, gap opening penalties, gap extension penalties, comparison matrices, thresholds of similarity, etc. can be used by those of skill in the art, including those set forth in the Program Manual, Wisconsin Package, Version 9, September 1997. The particular choices to be made will depend on the specific comparison to be made, such as DNA to DNA or RNA to DNA; and additionally, whether the comparison is between given pairs of sequences (in which case GAP or BestFit are generally preferred) or between one sequence and a large database of sequences (in which case FASTA or BLASTA are preferred).
• In an aspect, the disclosure provides a vector comprising the isolated polynucleotide as described herein such as, for example one or more of SEQ ID NOs 773-1046 or 1450-1542. In embodiments, the vector can be any type of vector that finds use as a vehicle to transfer foreign genetic material into a cell. Non-limiting examples of vectors include plasmids, viral vectors (e.g., derived from lentivirus, adenovirus, adeno-associated virus (AAV), retrovirus, etc.), bacteriophage, cosmids, and artificial chromosomes. In embodiments, the vector can be an expression (or expression constructs) for driving expression of the polynucleotide in a target cell. Vectors and methods for inserting them into a target cell are known in the art [See, e.g., Sambrook et al., 1989].
• In an aspect, the disclosure provides recombinant cells that comprise the vectors and/or polynucleotides described herein. The cells can be any cell suitable as a host for recombinant nucleic acid molecules, and selected based on well known techniques. Techniques for generating and maintaining recombinant cells are known in the art, such as those described in Sambrook et al., 1989.
• The term “B cell malignancy,” as used herein, refers to a malignancy derived from any stage of B cell, including, but not limited to, naïve cells, germinal center cells, memory B cells, and plasma cells. Examples of B cell malignancies include, but are not limited to, mantle cell lymphoma, follicular lymphoma, Hodgkin's lymphoma, Burkitt lymphoma, germinal center B-cell like diffuse large B cell lymphoma (DLBCL), chronic lymphocytic leukemia, small lymphocytic lymphoma, lymphoplasmacytic lymphoma, multiple myeloma, and activated B-cell like DLBCL.
• In an aspect, the disclosure provides a method of distinguishing B cell malignancies on the basis of the B-cell origin. In certain embodiments, methods of diagnosing B cell malignancies on the basis of the B-cell origin are provided. In certain such embodiments, a B cell malignancy is determined to be derived from a particular B-cell stage. The B-cell origin of a B cell malignancy may be determined, in certain embodiments, by detecting one or more microRNAs that can be used to distinguish B-cell stages. Certain exemplary B-cell stages include, but are not limited to, naïve cells, germinal center cells, memory B cells, and plasma cells. Certain exemplary microRNAs that can be used to distinguish B-cell stages are shown in Table 4. In various embodiments, the method comprises detecting at least one, at least two, at least five, at least 10, at least 20, at least 30, at least 50, at least 75, or at least 100 microRNAs.
• In certain embodiments, a panel of microRNAs is selected that will allow determination of the B cell stage from which a B cell malignancy is derived. For example, in certain embodiments, two or more microRNAs from Table 4 are selected such that detection of the levels of those microRNAs in a B cell malignancy will indicate whether the B cell malignancy is derived from naïve, germinal center, plasma, or memory B cells. In various embodiments, the panel of microRNAs comprises at least one, at least two, at least five, at least 10, at least 20, at least 30, at least 50, at least 75, or at least 100 microRNAs from Table 4. One skilled in the art can select a suitable panel of microRNAs, including one or more microRNAs from Table 4, according to the intended use of the panel.
• As described throughout the disclosure, the methods herein can include detecting one or a plurality of miRNAs. When the term “at least” is used in association with a number (e.g., “at least 20”) that term will be understood to include 20 as well as optionally any integer after 20 and up to and including the total number of microRNAs disclosed herein.
• In some embodiments, a B cell malignancy derived from naïve cells is mantle cell lymphoma. In other embodiments, a B cell malignancy derived from germinal center cells includes, but is not limited to, follicular lymphoma, Hodgkin's lymphoma, Burkitt lymphoma, or germinal center B-cell like diffuse large B cell lymphoma (DLBCL). In other embodiments, a B cell malignancy derived from memory B cells includes, but is not limited to, chronic lymphocytic leukemia or small lymphocytic lymphoma. In some embodiments, a B cell malignancy derived from plasma cells includes, but is not limited to, multiple myeloma or activated B-cell DLBCL.
• Certain B cell malignancies can be difficult to distinguish using current methodologies. In extreme cases, almost any B cell malignancy can be confused with another. As illustrative examples, Burkitt lymphoma and DLBCLs are often confused. Similarly, mantle cell lymphoma and small lymphocytic lymphoma can also be confused. Burkitt lymphoma and germinal center DLBCL are both derived from germinal center cells, while activated B-cell DLBCL is derived from plasma cells. Thus, if a B cell malignancy appears to be Burkitt lymphoma or a DLBCL, in certain embodiments, microRNA analysis can be used to narrow down the B cell malignancy to either a germinal center cell-derived B cell malignancy or a plasma cell-derived B cell malignancy. If the B cell malignancy is plasma-cell derived, then it may be activated B-cell DLBCL.
• Thus, in certain embodiments, when the identity of a particular B cell malignancy has been narrowed down to two or more possible B cell malignancies, and at least two of those B cell malignancies are derived from different B cell stages, microRNAs that distinguish certain B cell stages can be used to further narrow down the identity of the B cell malignancy. In certain embodiments, microRNAs that distinguish certain B cell stages can be used to identify the B cell malignancy. One or more such microRNAs can be selected, in certain embodiments, from the microRNAs in Table 4. One skilled in the art can select a suitable set of microRNAs, including at least one microRNA from Table 4, for distinguishing particular B cell stages.
• In an aspect, the disclosure provides a method of identifying a B cell malignancy comprising detecting one or a plurality of microRNAs. In certain embodiments, the method can provide a diagnosis of a B cell malignancy. In certain embodiments, one or more microRNAs that are characteristic of a particular B cell malignancy are used to identify the B cell malignancy. In certain embodiments, the identity of the B cell malignancy is first narrowed down to a list of two or more particular B cell malignancies using, for example, tumor morphology and/or immunohistochemistry and/or microRNA detection, e.g., to determine the B cell stage from which the tumor is derived. Certain exemplary microRNAs that can be used to identify B cell malignancies are shown in the Tables (e.g., Tables 7-35).
• In certain embodiments, methods of identifying B cell malignancies comprise detecting one or more microRNAs from one or more of Tables 7 to 15, and Appendix B, Tables 16 to 30. That is, in certain embodiments, a panel of microRNAs is selected that will identify a B cell malignancy as being one of a particular selection of B cell malignancies. As a non-limiting example, a panel of microRNAs can be designed to identify a B cell malignancy as one of Burkitt lymphoma, ABC DLBCL, or GCB DLBCL. In certain such embodiments, the panel of microRNAs comprises at least one microRNA from Table 10, column “BL miRNA list” and/or Table 14, column “BL High”; at least one microRNA from Table 11 and/or Table 14, column “ABC High”; and at least one microRNA from Table 10, column “GCB miRNA list” and/or Table 14, column “GCB High”.
• When other methods indicate a particular identity for a B cell malignancy, in certain embodiments, microRNAs can be used to confirm that identification. Thus, for example, if a B cell malignancy is believed to be a Burkitt lymphoma, that identification can be confirmed by determining the expression level of one or more microRNAs listed in Table 10, column “BL miRNA list” and/or Table 14, column “BL High”. Similarly, if a B cell malignancy is believed to be ABC DLBCL, that identification can be confirmed by determining the expression level of one or more microRNAs listed in Table 11 and/or Table 14, column “ABC High”. If a B cell malignancy is believed to be GCB DLBCL, that identification can be confirmed by determining the expression level of one or more microRNAs listed in Table 10, column “GCB miRNA list” and/or Table 14, column “GCB High”. If a B cell malignancy is believed to be chronic lymphocytic leukemia, that identification can be confirmed by determining the expression level of one or more microRNAs listed in Table 12 and/or Table 15, column “CLL High”. If a B cell malignancy is believed to be Hodgkin's lymphoma, that identification can be confirmed by determining the expression level of one or more microRNAs listed in Table 13 and/or Table 15, column “HL High”. If a B cell malignancy is believed to be follicular lymphoma, that identification can be confirmed by determining the expression level of one or more microRNAs listed in Table 10, column “FL miRNA list” and/or Table 15, column “FL High”.
• In certain embodiments, when the identity of a B cell malignancy has been narrowed down to two B cell malignancies selected from Burkitt lymphoma, GCB DLBCL, ABC DLBCL, chronic lymphocytic leukemia, follicular lymphoma, and Hodgkin's lymphoma, the identity of the B cell malignancy can be determined by detecting one or more microRNAs from Tables 16 to 30.
• As discussed in the Examples, the miRNAs described herein as differentially expressed in a B cell malignancy have been identified with high confidence, and thus, identification of one miRNA is adequate to perform the methods of identification and diagnosis disclosed herein. Accordingly, in various embodiments, the methods can comprise detecting at least one, at least two, at least five, at least 10, at least 20, at least 30, or at least 50 microRNAs in order to narrow down the identity of, or identify, a B cell malignancy.
• As noted above, the treatment regimens and prognoses for the various B cell malignancies can differ significantly. Thus, determining the correct identity and/or origin of a B cell malignancy can be important for selecting an effective therapy and/or setting appropriate patient expectations.
• B cell malignancy samples may be obtained and prepared using methods known in the art. One skilled in the art can select an appropriate method of obtaining a B cell malignancy sample according to various parameters, such as the age, size, medical history, and/or identity of the patient. One skilled in the art can select an appropriate method of preparing a B cell malignancy sample for analysis according to the B cell malignancy sample source, size, quality, and/or intended use. For example, in certain embodiments, a B cell malignancy sample is prepared in a manner that preserves microRNAs in the sample as much as practicable under the circumstances.
• MicroRNAs can be detected using any method known in the art. Exemplary methods of detecting microRNAs include, but are not limited to, hybridization-based methods and amplification-based methods. Certain exemplary detection methods include, but are not limited to, arrays (including microarrays and bead-based arrays), in situ hybridization, Northern blotting, TaqMan probes, RT-PCR, real-time PCR, and direct sequencing. One skilled in the art can select a suitable detection method according to the sample source, size, quality, and/or particular application.
• In certain embodiments, real-time PCR is employed to determine the expression level of a microRNA. In some embodiments a miRNA is considered present in a subpopulation if the cycling time (CT) is less than 36 in all three biological replicates, and a CT greater than 36 is undetected.
• In certain embodiments, the expression level of a microRNA in a sample is determined relative to a control sample. A control sample may be selected, in various embodiments, because it is expected to have either high or low expression of the microRNA.
• In certain embodiments, the expression level of a microRNA may be normalized to the expression level of a polynucleotide that is expected to be expressed at similar levels in several different cell types and/or at constant levels in the cell type being analyzed.
• In certain embodiments, an identified miRNA from Tables 7-35 is used to distinguish one of the six exemplified B cell malignancies from the other malignancies. A “high” and a “low” in Tables 10 to 13 refer to at least a 2-fold difference in the expression of the identified miRNA when one lymphoma is compared to other lymphomas and benign lymph nodes.
• In embodiments, mRNA levels can be profiled by using a microarray. In some embodiments, array elements with median signal intensities of less than 7 log 2 units across samples are removed from analysis. In embodiments, a gene is considered for further analysis if it is on-average 2-fold or higher differentially expressed in a binary comparison of B cell subsets and expressed in at least one of the two B cell subsets being compared.
• In an embodiment, the level of at least one miRNA is measured by reverse transcribing RNA from a test sample obtained from a subject to provide a set of target oligodeoxynucleotides, hybridizing the target oligodeoxynucleotides to one or more miRNA-specific probe oligonucleotides (e.g., a microarray that comprises miRNA-specific probe oligonucleotides) to provide a hybridization profile for the test sample, and comparing the test sample hybridization profile to a hybridization profile generated from a control sample. An alteration in the signal of at least one miRNA in the test sample relative to the control sample is indicative of the subject either having, or being at risk for developing, a B-cell malignancy. In an embodiment, the signal of at least one miRNA is upregulated, relative to the signal generated from the control sample. In another embodiment, the signal of at least one miRNA is down-regulated, relative to the signal generated from the control sample. In some embodiments, the microarray comprises miRNA-specific probe oligonucleotides for a substantial portion of all known human miRNAs. In a further embodiment, the microarray comprises miRNA-specific probe oligonucleotides for one or more miRNAs selected from the group consisting of SEQ ID NOs: 763-1350, or 1565 and any combination thereof.
• The microarray can be prepared from gene-specific oligonucleotide probes generated from known miRNA sequences. The array may contain two different oligonucleotide probes for each miRNA, one containing the active, mature sequence and the other being specific for the precursor of the miRNA. The array may also contain controls, such as one or more mouse sequences differing from human orthologs by only a few bases, which can serve as controls for hybridization stringency conditions. tRNAs and other RNAs (e.g., rRNAs, mRNAs) from both species may also be printed on the microchip, providing an internal, relatively stable, positive control for specific hybridization. One or more appropriate controls for non-specific hybridization may also be included on the microchip. For this purpose, sequences are selected based upon the absence of any homology with any known miRNAs.
• The microarray may be fabricated using techniques known in the art. For example, probe oligonucleotides of an appropriate length, e.g., 40 nucleotides, are 5′-amine modified at position C6 and printed using commercially available microarray systems. Labeled cDNA corresponding to the target RNA sequence(s) is prepared by reverse transcribing the target RNA with labeled primer. Following first strand synthesis, the RNA/DNA hybrids are denatured to degrade the RNA templates. The labeled target cDNAs thus prepared are then hybridized to the microarray chip under typical hybridizing conditions. At positions on the array where the immobilized probe DNA recognizes a complementary target cDNA in the sample, hybridization occurs. The labeled target cDNA marks the exact position on the array where binding occurs, allowing automatic detection and quantification. The output consists of a list of hybridization events, indicating the relative abundance of specific cDNA sequences, and therefore the relative abundance of the corresponding complementary miRNAs, in the patient sample.
• In an aspect, the disclosure relates to kits. Such kits can be used in methods of identifying a miRNA or mRNA described herein; an expression level or expression pattern of one or more miRNA(s) or mRNA(s) described herein; and/or identifying a B-cell malignancy. In some embodiments the kit can provide a diagnosis of a B-cell type and or a B-cell malignancy. In some embodiments the kit can differentiate one B-cell malignancy from other B-cell malignancies (e.g., ABC-DLBCL from GBC-DLBCL), and can provide information useful to a medical professional regarding a preferred course of therapeutic treatment. Suitably, a kit can comprise an isolated nucleic acid molecule or a plurality of isolated nucleic acid molecules as described herein (e.g., a sequence complementary to any of the miRNAs disclosed in the Tables). In embodiments, the isolated nucleic acid molecule can comprise a sequence of one or more RT-PCR target sequences, primers directed thereto, or a sequence complementary thereto. The kit can also include adapter nucleic acid molecules (e.g., universal adapter molecules for attachment to expressed miRNAs/mRNAs for reverse transcription and amplification); appropriate buffer systems and reagents, detectable labels, an energy source (e.g., ATP), and other agents and components that can be used in performing analysis of miRNA expression (e.g., in RT-PCR, deep sequencing, or microarray-based methods). Kits also include instructions for use.
• It will be understood that any numerical value recited herein includes all values from the lower value to the upper value. For example, if a concentration range is stated as 1% to 50%, it is intended that values such as 2% to 40%, 10% to 30%, or 1% to 3%, etc., are expressly enumerated in this specification. These are only examples of what is specifically intended, and all possible combinations of numerical values between the lowest value and the highest value enumerated are to be considered to be expressly stated in this application.
• In an aspect, the disclosure provides a DNA library comprising one or more miRNA sequences from Tables 4-5, or 6-33. In a related aspect, the disclosure provides a method for generating such a DNA library. In an embodiment the library comprises a cDNA library that includes sequences derived from a sample of the miRNAs or, in addition or alternatively, the mRNA purified from a particular source such as, for example, a collection of cells, a particular tissue, or an entire organism. In embodiments, the source of the cDNA library is a B cell, such as a B cell in any stage (e.g., naïve, germinal center, memory, activated, or plasma, etc.) or a B cell malignancy (e.g., mantle cell lymphoma, follicular lymphoma, Hodgkin's lymphoma, Burkitt lymphoma, germinal center B-cell like diffuse large B cell lymphoma (DLBCL), chronic lymphocytic leukemia, small lymphocytic lymphoma, lymphoplasmacytic lymphoma, multiple myeloma, and activated B-cell like DLBCL). Typically, the isolated miRNA (or mRNA) is converted to a DNA template by reverse transcription, and comprises the cDNA version of the expressed RNA (e.g., miRNA or mRNA). Thus, a library can represent the cDNA version of the active “transcriptome” in a particular source under the physiological, developmental, or environmental conditions that existed when the miRNA/mRNA was purified.
• In an embodiment, the library comprises a miRNA sequence described in Tables 4-5; 7-33. In embodiments, the library comprises at least one of SEQ ID NOs 763-1350 or 1565, and any combination thereof. In an embodiment, the library comprises a collection of miRNA sequences comprising SEQ ID NOs 763-1350 or 1565. In embodiments the library can be used to identify and/or differentiate a B-cell malignancy from other B-cell malignancies. In such embodiments, the library comprises at least one miRNA sequence selected from those listed in any of Tables 4 or 7-35.
• As used herein, a “library” is a collection of DNA sequences that is stored and propagated in a population of microorganisms through standard molecular cloning processes. A DNA library can be of any type such as, for example, a cDNA library (formed from reverse-transcribed RNA) or a genomic library (formed from genomic DNA). The DNA library can be used in any routine application or technique known in the art (e.g., gene discovery; cloning of full-length cDNAs to identify/study gene function; miRNA/mRNA expression in different cells or tissues; splice variants in different cells or tissues) and, in some embodiments, can depend on the source of the original DNA fragments. In embodiments, the library can be used to isolate, characterize, and/or quantify the actively expressed miRNA is a population of cells such as, for example, B-cells or B-cell malignancies. In some embodiments, the library can be used to study miRNA-protein interactions or miRNA-based regulation of protein expression or activity.
• Any known method of library preparation can be used to make the library described herein, including the methods described in the detailed description and non-limiting Examples. Further general techniques can be based on the methods and techniques known in the art, (see, e.g., RNA Methodologies: A Laboratory Guide for Isolation and Characterization (R. E. Farrell, Academic Press, 1998); cDNA Library Protocols (Cowell & Austin, eds., Humana Press; Functional Genomics (Hunt & Livesey, eds., 2000); and the Annual Review of Genomics and Human Genetics (E. Lander, ed., yearly publications by Annual Reviews). Suitably, the nucleotide sequences of interest in a library are preserved as inserts in a plasmid or the genome of a bacteriophage that has been used to infect bacterial cells. There are differences in the cloning vectors and techniques used in library preparation, but in general each DNA fragment is uniquely inserted into a cloning vector and the pool of recombinant DNA molecules is then transferred into a population of bacteria or yeast such that each organism contains on average one construct (vector+insert). The DNA molecules are copied and propagated along with the population of organisms in culture (thus, effectively, “cloned”). Accordingly, in some embodiments, the term “library” can refer to a population of organisms, each of which carries a DNA molecule inserted into a cloning vector, or alternatively to the collection of all of the cloned vector molecules.
• An “increased level” of expression, as used herein, refers to a level of expression that is at least 2-fold greater than the level of expression in a control cell type or tissue. In various embodiments, the level of expression is at least 2.5-fold, at least 3-fold, at least 5-fold, or at least 10-fold, greater than the level of expression in a control cell. Exemplary control cells and tissues include, but are not limited to, normal cells, benign lymph nodes, and other B cell malignancies. In certain embodiments, benign lymph nodes are used as a control tissue. Such benign lymph node tissue contains a variety of cell types.
• A “decreased level” of expression, as used herein, refers to a level of expression that is less than 50% of the level of expression in a control cell.
• The term “differentially expressed” or “differential expression” relates to a difference in the observed or detected level of expression of a biomolecule such as, for example, nucleic acids (e.g., a polynucleotide, mRNA, miRNA, etc.) or amino acid sequence (e.g., protein, polypeptide, etc.) in a test sample relative to the observed or detected level of expression of the same biomolecule in a control sample or other reference (e.g., a previously established reference level). The difference in expression can be either an increase or a decrease in the expression of the biomolecule in the test sample relative to the control sample.
• The Examples that follow provide further illustration of certain aspects and embodiments described in the foregoing description. These illustrative Examples should not be interpreted as limiting the scope of the appended claims.
EXAMPLES Example 1 Materials and Methods
• Patient Sample Processing
• B cell populations were obtained from young patients undergoing routine tonsillectomy using a protocol approved by the Clinical Center at the National Institutes of Health. Patient tonsils were disaggregated and separated by Ficoll. The mononuclear cell layer was harvested, washed in PBS, and resuspended in ACK lysing buffer to remove small numbers of red blood cells. After a wash and resuspension with 10 ml of PBS with 10% Bovine Serum Albumin, cells were counted and 200 million were stained with fluorochrome-tagged monoclonal antibodies to CDI9, IgD, CD38 and CD27. The specific monoclonal antibodies employed were anti-CDI9-PE-Cy5.5, anti-IgD-FITC, anti-CD27-PE, and anti-CD38-APC, all from BD Biosciences and BD Pharmingen (San Jose Calif.). Cells were sorted using the MoFlo Cell sorter (Dako Cytomation, Colorado Springs, Colo.) into naive B cells (CDI9+IgD+CD2TCD38+), germinal center B cells (CDI9+IgD−CD38++), memory B cells (CDI9+IgD−CD27+CD38dim) and plasma cells (CDI9dimIgD−CD27++CD38+++). Three replicates of each B cell subset were obtained from separate patients. The sample purity was verified by FACS and found to be over 90% in all cases.
• Tumor specimens were obtained from patients who were examined under a protocol approved by the Duke University Medical Center Institutional Review Board. The pathologic diagnosis of the samples was verified prior to analysis. Samples from patients with diffuse large B cell lymphoma were further subclassified as described previously. See Hans et al. (2004) Blood 103: 275-282. Chronic lymphocytic leukemia samples were processed and purified as described previously. See Volkheimer et al. (2007) Blood 109: 1559-1567. Total RNA was extracted using the phenol-chloroform method to preserve miRNAs, using Ambion reagents.
• microRNA Profiling Using Multiplexed Real-Time PCR
• MiRNA expression profiling was conducted using the Applied Biosystems 384-well multiplexed real-time PCR assay using 400 ng of total RNA. Eight reactions, each containing 50 ng of RNA and a multiplex looped primer pool with endogenous small nucleolar (sno)-RNA controls, were used to reverse-transcribe the miRNAs in parallel fashion. Each completed reaction was loaded onto the 384-well plate per manufacturer's instructions, and real-time PCR was run on the ABI 7900HT Prism. For each 384-well plate, we used the automatically determined cycle-threshold (CT) using the SDS 2.2.1 software (Applied Biosystems). Consistent with manufacturer recommendations, we considered CT greater than 36 as undetected. A miRNA was considered to be present in a subpopulation if the CT was less than 36 in all three biological replicates. The probes deemed to be present were normalized to the average expression of a sno-RNA control. The expression values were calculated as 2^−ΔCT, then median centered to 500 and log 2-transformed.
• Gene Expression (mRNA) Profiling Using Microarrays
• Gene expression profiling and normalization were performed using methods identical to those we have described previously. See Dave et al. (2004) N. Engl. J. Med. 351: 2159-2169. Array elements with median signal intensities of less than 7 log 2 units across the samples were removed from analysis, in order to exclude poorly measured genes and genes not appreciably expressed in the samples. Genes that were on-average 2-fold or higher differentially expressed in a binary comparison of B cell subsets, and appreciably expressed in at least one of the 2 B cell subsets being compared, were selected for further analysis as described below. The data have been deposited in the publicly available Gene Expression Omnibus database (GSE12366).
• MiRNA Profiling Using Microarray
• MiRNA expression profiling from human B cell malignancies was conducted using up to 1 μg of total RNA from sample and reference (normal lymph node), which were labeled with Cy3 or Cy5 fluorescent dyes, using the miRNA/LNA labeling kit (Exiqon, Denmark). The fluorescently labeled samples were combined and hybridized to a miRNA microarray (v.10.0, Exiqon, Denmark), in a nitrogen atmosphere. The micro array slides were scanned with GenePix 4100 Scanner. The quantified signals were normalized using the global Lowess algorithm, using Genespring (Agilent) software. The intensity values for multiple spots were averaged and the normalized values were log 2-transformed. Missing values were replaced with the lowest value for analysis.
• MiRNA Target Prediction
• Annotated genes on the U133plus 2.0 array were matched to the miRNA target list downloaded from TargetScan (www.targetscan.org). For the purpose of this study, a target gene was defined by the presence of a seed sequence match (nucleotides 2-8) and conservation of the seed sequence and 3 ′UTR in humans, dog, rat, mouse and chicken. Additional conservation was examined in miRNA target genes selected for experimental validation. The distribution of the mRNA expression for these genes was plotted as a density plot using the Splus statistical software (Insightful Corporation). The difference in distribution between the B cell subsets was calculated using a two-sample, 1-sided Kolmogorov-Smirnov test to examine the hypothesis that being a miRNA target conferred repression in the appropriate population (consistent with the known biology of miRNA effects).
• The 3′UTRs of LM02, MYBL1 and PRDM1 were aligned using Blastz alignment of Human, Chimp, Mouse, Rat, Dog, Chicken, Frog (Xenopus) and Zebrafish, and were displayed using the UCSC genome browser. The conservation of miR-223 seed sequence and the 3′UTRs of LM02 and MYBL1, as well as that of the miR-30 family and miR-9 on PRDM1, were thus verified.
• Western Blot
• RIPA Lysis buffer (1× phosphate-buffered saline [PBS], 1 Nonidet P-40, 0.5% sodium deoxycholate, 0.1% SDS, 10 mM phenylmethylsulfonyl fluoride, 1 μg/mL aprotinin, and 100 mM sodium orthovanadate) was added to 750,000 cells and incubated on ice for 30 minutes. The mixture was spun down and the supernatant was transferred to a new tube as the whole cell extract. A total of 20 μg of cell lysate was separated on a 4-18% Tris-Bis NuPAGE gel (Invitrogen) and transferred using the iBlot transfer device (Invitrogen) program 3 for 7 minutes (LM02 detection) or program 2 for 6 minutes (PRDM1). The blots were probed using 1:200 mouse-anti-LM02 (Santa Cruz Biotechnologies SC-65736), 1:750 mouse-anti-Blimp-1 (Santa Cruz Biotechnologies SC-66015) or 1:5000 goat-anti-B-actin (Santa Cruz Biotechnologies SC-47778) for 1 hour at room temperature. The antibodies were detected using 1:10,000 goat-anti-mouse horseradish peroxidase conjugated antibodies (Santa Cruz Biotechnologies). Western Blotting Luminol Reagent (Santa Cruz Biotechnologies) was used to visualize the bands corresponding to each antibody.
• Single miRNA/mRNA Expression Using Real-Time Polymerase Chain Reaction (RT-PCR)
• With 10 ng of RNA per reaction, miRNAs of interest were reverse-transcribed with ABI individual stem-loop primers designed to detect only mature miRNA, and measured by Taqman real-time PCR normalized to the small nucleolar RNA, RNU48. In order to assess mRNA expression using RT-PCR, 1 μg of RNA was reverse-transcribed with the ABI High Capacity cDNA Reverse Transcription kit. Gene expression was measured with exon-spanning Taqman probes, and normalized to beta-2 micro globulin expression.
• Cell Culture
• BJAB and H929 were cultured in RPMI (Gibco) supplemented with 10% fetal bovine serum, and U266 was cultured in RPMI supplemented with 15% fetal bovine serum. 293T cells were grown in DMEM media (Gibco) with 10% FBS. All cell lines were grown in 37° C. humidified cell culture incubators with CO₂ maintained at 5%.
• MiRNA Functional Analysis
• MicroRNA Transfection
• miRNAs of interest were over-expressed in cell lines of interest by transfecting the appropriate miRNA precursors (Ambion) at 100 nanomoles using Amaxa's Nucleofector system. In particular, BJAB was transfected with Nucleofector solution T, program T-016, U266 with Nucleofector C, program X-005, and H929 with Nucleofector V, program T-001. 1.5 million cells were used per transfection and mixed with appropriate miRNA precursors (Ambion) for a concentration of 100 nM.
• Statistical Analysis
• Identifying Differentially Expressed miRNA and mRNA
• MiRNAs were considered to be differentially expressed if the mean signal was changed at least 2-fold and a false discovery rate (q) was less than 5% using Significance Analysis of Microarrays (SAM) with 1000 permutations. See Tusher et al. Proc Natl Acad Sci USA. 2001; 98:5116-5121.
• Differentially expressed genes (mRNA) in Naive versus Germinal Center, Germinal Center versus Plasma Cells, and Germinal Center versus Memory Cells comparisons were identified using SAM. Genes that were 2-fold differentially expressed at a false discovery rate (q) less than 1% with 1000 permutations were identified as significantly differentially expressed.
• Transcription Factors and miRNA Target Genes
• Transcription factors were identified based on the gene ontology (GO search term “transcription factor”) and matched to the probes of the Affymetrix U133plus 2.0 microarray. Of the total of 938 transcription factor genes thus identified, we selected 364 genes that were differentially expressed in at least one of the B cell stage transitions. We evaluated the breakdown of the differentially expressed transcription factors among miRNA targets versus non targets. The p-values were computed using a chi-square test separately in each B cell stage-transition.
• B Cell Malignancy Sample Classification
• The top 50 most differentially expressed miRNAs (P<0.01) in each pair-wise B cell malignancy type comparison were chosen as the initial predictor. Singular value decomposition was applied to reduce the list to 20 most informative miRNAs in each pair-wise comparison. See West et al. Proc Natl Acad Sci USA. 2001; 98:11462-11467. A Bayesian logistic regression was performed in Matlab (Mathworks) using the 20-predictor miRNAs for each pair-wise comparison. Each sample was tested using the microRNA-based predictor in a leave-one-out fashion to determine the accuracy of each prediction. For a sample to be classified as a particular B cell malignancy (or normal) type, it had to be predicted as such in every pair-wise comparison.
• Normal B Cell Stage Classification of B Cell Malignancies
• We constructed a Bayesian predictor to distinguish normal naive from germinal center B cells based on the 32 miRNAs depicted in FIG. 1D. We then applied the predictor without optimization to the microarray data generated for GCB DLCBL, Burkitt lymphoma and chronic lymphocytic leukemia to render a Bayesian prediction of lineage; i.e. naive versus germinal center B cell.
• Western Blot Quantitative Analysis
• Western blot scans were quantified using NIH ImageJ software. For each experiment, the ratios of protein of interest (LM02, PRDM1) to Actin were determined and mean centered to 100 across the experiment. The average and standard deviation of these values across the three experiments were calculated and displayed relative to the scrambled control expression.
• Luciferase Indicator Assay Quantitative Analysis
• Firefly luciferase reporter constructs were created in the pL/SV40/GL3 vector for the LM02 3′UTR and the LM02 3′UTR with the predicted miR-223 binding site mutated, as described below. Mature microRNA expression of a pL/CMV/eGFP vector coding for pri-miR-223 from the 3′UTR of EGFP of the vector was confirmed by Taqman-real time PCR in transfected 293T cells. gl3 activity was normalized in dual luciferase assays to pL/SV40/RLuc, with which it was cotransfected. The PRDM1 3′UTR was also cloned into the pL/SV40/GL3 vector. microRNA expression vectors and their respective seed sequence mutants were created for miR-9-2, miR-30b, and miR-30d.
• LM02
• The LM02 3′UTR was PCR-amplified from BJAB cDNA using primers 1 and 2 (SEQ ID NOs: 1 and 2, respectively) and ligated into the XhoI and XbaI sites of the previously described lentiviral vector pL/SV40/GL3, which expresses firefly luciferase. See Tusher et al. Proc Natl Acad Sci USA. 2001; 98:5116-5121. As a control, an LM02 3′UTR mutant was created using mutant PCR primers 3 and 4 and then outer primers 1 and 2. The resulting fragment was also placed into the XhoI and XbaI sites of pL/SV40/GL3. In this LM02 3′UTR mutant, the seed match predicted to bind to nucleotides 2-8 of miR-223 is converted from 5′AACUGAC 3′ to 5′AACAGUC 3′. To create a miR-223 expression vector, a ˜350 nucleotide-long fragment of pri-miR-223, encompassing the pre-miRNA stem loop in its middle, was PCR-amplified from genomic BJAB DNA with primers 6 and 7 (SEQ ID NOs. 6 and 7) and ligated into the XhoI and XbaI sites of the pL/CMV/eGFP vector. This pL/CMV/eGFP vector was generated by ligating a fragment containing the CMV promoter and the EGFP ORF into the BamHI and XhoI sites of the previously described lentiviral backbone pL. See Tusher et al. Proc Natl Acad Sci USA. 2001; 98:5116-5121. The expression of miR-223 from the 3′UTR of EGFP in the resulting vector was confirmed by Taqman real time PCR in transfected 293T cells.
• For luciferase indicator assays, 293T cells plated in 24 well plates were transfected using FUGENE6 as follows:

• TABLE 1
  Luciferase indicator assay compositions

  2.5 ng pL/SV40/GL3	2.5 ng pL/SV40/GL3/	2.5 ng pL/SV40/GL3/
  2.5 ng pL/SV40/RLuc	LMO2	LMO2 seed
  0.4 μg pL/CMV/	UTR	mut
  eGFP/miR-223	2.5 ng pL/SV40/RLuc	2.5 ng pL/SV40/RLuc
  2.5 ng pL/SV40/GL3	0.4 μg pL/CMV/	0.4 μg pL/CMV/eGFP/
  2.5 ng pL/SV40/RLuc	eGFP/miR-223	miR-223
  0.4 μg pL/CMV/eGFP	2.5 ng pL/SV40/	2.5 ng pL/SV40/GL3/
  	GL3/LMO2	LMO2 seed
  	UTR	mut
  	2.5 ng pL/SV40/RLuc	2.5 ng pL/SV40/RLuc
  	0.4 μg pL/CMV/eGFP	0.4 μg pL/CMV/eGFP

• Reporter expression was evaluated by dual luciferase assays (Promega) 48 hours post-transfection. Firefly Luciferase (GL3) to internal control Renilla Luciferase (RLuc) ratios from 293T cells transfected with pL/CMV/eGFP/miR-223 were divided by those obtained from 293T transfected with the pL/CMV/eGFP vector control. The average and standard deviation were taken across five experiments for the pL/SV40/gl3 empty, LM02, and LM02 mutant vectors.
• Firefly Luciferase (GL3) activity readings of the PRDM1 3′UTR construct were divided by internal control Renilla Luciferase (RLuc) activity readings. The average and standard deviation of these ratios across three experiments were calculated and scaled relative to the empty vector (pL/CMV/eGFP) transfection.

• TABLE 2
  LM02 primer sequences

  Primer
  SEQ ID	description	Sequence (5′ → 3′)
  1543	LMO2 3′UTR, FW	ATATCTCGAGGCCCGAGTCCCCGGGCATCTTTGG
  1544	LMO2 3′UTR, REV	ATATATCTAGACTACACACGACAAATACTTTG
  1545	LMO2 3′UTR seed	CAGCCCATCCATAGTAACAGTCATGATTAGCAGAAGAAAGG
  	mutant, FW
  1546	LMO2 3′UTR seed	CCTTTCTTCTGCTAATCATGACTGTTACTATGGATGGGCTG
  	mutant, REV
  1547	pri-mir-223, FW	ATATCTCGAGGGTCACATCTCCCAGGAAGATC
  1548	pri-mir-223, REV	ATATATCTAGAAGCACTCTCATGGTGTGTGTAG

• PRDM1
• The PRDM1 3′UTR was PCR-amplified from BJAB genomic DNA in two reactions using primer pairs 7 and 8 in one reaction, and 9 and 10 in another (SEQ ID NOs: 7 to 10, respectively). The two fragments were then ligated together into the XhoI and NotI sites of pL/SV40/GL3 to generate the entire PRDM1 3′UTR. microRNA expression vectors were created as described above for mir-9-2, mir-30b, and mir-30d with the primers listed below. Again, over-expression from the 3′UTR of EGFP in the resulting vector was confirmed by Taqman real-time PCR in transfected 293T cells. For the PRDM1 luciferase assays, we used the sequences listed below to PCR-mutate the seed sequence of the microRNA expression vectors rather than the PRDM1 3′UTR, which had numerous seed sequence binding sites.

• TABLE 3
  PRDM1 primer sequences

  Primer
  SEQ ID:	description	sequence
  1549	PRDM1 3′UTR piece 1, FW	AGAGACTCGAGGATTTTCAGAAAACACTTATTT
  1550	PRDM1 3′UTR piece 1, REV	TTGCTTCTCTAGAGGAGAAAC
  1551	PRDM1 3′UTR piece 2, FW	GTTTCTCCTCTAGAGAAGCAA
  1552	PRDM1 3′UTR piece 2, REV	AGAGAGCGGCCGCAGGGGAGAGACAAATTGCATTG
  1553	pri-mir-9-2, FW	AGAGACTCGAGATAAAAGGAGGAATCTTAAG
  1554	pri-mir-9-2, REV	AGAGAGCGGCCGCGAAAAAAACAAAACAAAAACAA
  1555	pri-mir-30b, FW	AGAGAGCGGCCGCCCGATTGAGTCTTGCCTCAT
  1556	pri-mir-30b, REV	AGAGAGAATTCAATGGTCTCACATTTCCAAC
  1557	pri-mir-30d, FW	AGAGAGCGGCCGCATGTCACAGCTATTGTTCAG
  1558	pri-mir-30d, REV	AGAGAGAATTCGCAGTAAAAGAATGCAGCTA
  1559	pri-mir-9-2 seed mutant, FW	GGAAGCGAGTTGTTATCTATGCTTATCTAGCTGTATGAGT
  1560	pri-mir-9-2 seed mutant, REV	ACTCATACAGCTAGATAAGCATAGATAACAACTCGCTTCC
  1561	pri-mir-30b seed mutant, FW	ACCAAGTTTCAGTTCATGTTAAGATCCTACACTCAGCTGT
  1562	pri-mir-30b seed mutant, REV	ACAGCTGAGTGTAGGATCTTAACATGAACTGAAACTTGGT
  1563	pri-mir-30d seed mutant, FW	CAGAAAGTCTGTTGTTGTTAAGATCCCCGACTGGAAGCTG
  1564	pri-mir-30d seed mutant, REV	CAGCTTCCAGTCGGGGATCTTAACAACAACAGACTTTCTG

• Luciferase assays were carried out in a manner similar to those described for LMO2.
• IgVH Mutation Status of Chronic Lymphocytic Leukemia Samples
• IgVH mutation status was determined as described in Volkheimer et al. (Blood. 2007; 109:1559-1567) using genomic DNA. In brief, genomic DNA was isolated from purified CLL cells and isolated using the GenElute Mammalian DNA extraction Kit from Sigma (St. Louis, Mo.) according to the manufacturer's instructions. DNA was amplified using nested PCR primers. PCR products were electrophoresed, purified, and sequenced using an automated DNA sequencer (Applied Biosystems, Foster City, Calif.) with the BigDye Terminator kit (Perkin Elmer, Boston, Mass.). Forward and reverse sequences were aligned into a single resolved sequence using Sequencher 4.1 software (Gene Codes Corporation, Ann Arbor, Mich.), and then aligned with germline sequences derived from DNA Plot on the V BASE directory website (http://vbase.mrc-cpe.cam.ac.uk/). The percent sequence identity was calculated by dividing the number of mutations from FR1 to FR3 by the total number of nucleotides in this region. Samples were considered somatically mutated if they had greater than 2% mutations in this region.
Example 2 Mature B Cell Stages Display Characteristic Patterns of MicroRNA Expression
• Mature B cell subsets can be defined by the expression of surface CD19, IgD, CD38, and CD27, and were obtained by fluorescence activated cell sorting of tonsils from young individuals undergoing routine tonsillectomy. See FIGS. 1B and 1C. Cells were previously gated on CD19 positive cells. Naive and memory B cells were distinguished from germinal center and plasma cells based on surface CD38 and IgD expression.
• To determine whether mature B cell subsets had unique patterns of microRNA (miRNA) expression, we used a 384-well multiplexed real time polymerase chain reaction (RT-PCR) assay (Applied Biosystems) that allowed measurement of all 365 miRNAs in miRBase 9.2. See Chen et al. Nucleic Acids Res. 2005; 33:e179; and He et al. Nature. 2007; 447:1130-1134. We detected a total of 113 unique miRNAs in the B cell populations. See Table 4. This detection frequency compares favorably to the identification of 71 unique miRNAs (45 miRNAs with more than one clone) through the examination of 3101 sequences cloned from unselected CD19-positive mature B cells. See Landgraf et al. Cell. 2007; 129:1401-1414. We identified differentially expressed miRNAs in mature B cell subsets using a false discovery rate of less than 5%. See FIGS. 1D, 1F, and 1H. The complete list of assayed miRNAs found to be expressed in the B cell populations is shown in Tables 4 and 5.
• In Table 4, normalized expression values for B-cell subsets are shown, along with significance analysis of microarrays q values. Empty microRNA expression cells indicate below-threshold values, defined as RT-QPCR Ct values greater than 36 or undetected. SAM q values greater than 5 were considered non-significant and not displayed.

• TABLE 4
  MicroRNAs detected in at least one B-cell subset

  					qNaive	qGerminal	qGerminal
  		Germinal			vs	Center	Center
  	Naïve	Center	Plasma Cell	Memory	Germinal	vs	vs
  	average	average	average	average	Center	Plasma Cell	Memory

  hsa-let-7a	11.7	9.2		11.2			1.4
  hsa-let-7b	10.3	9.0	7.7	11.3			0.0
  hsa-let-7c	7.3			7.2
  hsa-let-7d	9.0	9.0		10.0
  hsa-let-7f	10.3	7.6	8.4	10.2			0.0
  hsa-let-7g	13.1	10.3	10.5	14.0	0.0		0.0
  hsa-miR-100				7.4
  hsa-miR-101	8.4			10.0
  hsa-miR-103	9.7	11.4	9.8	10.8	0.0	0.0
  hsa-miR-106b	11.7	12.5	11.6	12.7
  hsa-miR-125a			6.7	8.3
  hsa-miR-125b		6.3		7.3
  hsa-miR-130b	7.8	9.8	8.6	10.2	3.6	4.5
  hsa-miR-132		7.5		8.9
  hsa-miR-133b	7.7	7.7	7.2	7.3
  hsa-miR-140	10.8	12.0	11.1	12.0	4.7
  hsa-miR-141	7.0			6.2
  hsa-miR-142-3p	16.9	17.0	15.3	18.0		1.1
  hsa-miR-142-5p	11.7	11.0	9.8	13.4			2.1
  hsa-miR-146a	10.1	14.0	14.0	14.6	0.0
  hsa-miR-146b	9.2	9.2	8.1	10.5			2.1
  hsa-miR-148a	6.7	9.4	11.9	9.0	0.0
  hsa-miR-148b	6.4			8.5
  hsa-miR-151	8.2	9.6	6.6	9.0	1.5	0.0
  hsa-miR-152	6.4		9.7	6.6
  hsa-miR-155	14.2	14.6	15.0	15.6
  hsa-miR-15a	10.2	10.1	7.8	10.8		2.0
  hsa-miR-15b	11.2	14.0	12.6	12.9	0.0	2.8
  hsa-miR-16	16.7	17.3	17.1	17.1
  hsa-miR-17-3p				9.1
  hsa-miR-17-5p	8.8	11.5	8.9	9.4	0.0	0.0	0.0
  hsa-miR-181b	9.0	11.7	10.8	9.3	0.0		0.0
  hsa-miR-181d	8.2	10.6	8.5	9.0	0.0	1.1
  hsa-miR-182			8.7	8.7
  hsa-miR-186	8.8	9.2	8.7	9.7
  hsa-miR-18a	5.7	8.9	6.8	7.4	0.0	0.0
  hsa-miR-191	11.9	12.7	12.4	13.3
  hsa-miR-192	9.1	9.0	8.8	10.1
  hsa-miR-193b				6.6
  hsa-miR-194	6.8	7.4	4.9	9.5		2.0	4.7
  hsa-miR-195	10.3	8.7	8.8	9.2
  hsa-miR-197	9.7	10.5	8.7	11.0		0.0
  hsa-miR-199a*				7.0
  hsa-miR-19a	9.7	12.8	11.9	11.1	0.0
  hsa-miR-19b	14.0	15.6	14.9	15.0	0.8
  hsa-miR-200a	6.5			6.8
  hsa-miR-200b				7.5
  hsa-miR-200c	9.0	9.6		9.7
  hsa-miR-203	7.9			7.6
  hsa-miR-20a	12.5	14.9	12.2	13.8	0.0	0.0
  hsa-miR-20b	8.4	11.0	8.0	8.5	0.0	1.1	0.0
  hsa-miR-21	12.6	13.0	11.2	15.1		2.8	2.1
  hsa-miR-210	9.2	11.1	10.7	10.2	0.8
  hsa-miR-214				7.6
  hsa-miR-22			8.3	7.4
  hsa-miR-221				6.9
  hsa-miR-222	11.4	11.6	7.8	12.7		0.0	4.7
  hsa-miR-223	13.7	10.4	9.6	14.6	0.0		0.0
  hsa-miR-23a				8.7
  hsa-miR-23b				6.2
  hsa-miR-24	11.7	11.8	12.8	13.4			2.1
  hsa-miR-25	9.7	10.8	8.8	11.2		2.8
  hsa-miR-26a	14.7	13.6	14.2	16.3			0.0
  hsa-miR-26b	12.6	12.1	12.0	14.1			3.4
  hsa-miR-27a		6.5		11.1			0.0
  hsa-miR-27b				7.2
  hsa-miR-28	9.4	13.3	8.4	10.3	0.0	0.0	0.0
  hsa-miR-296	6.9	8.1	6.6	7.9	0.0	2.0
  hsa-miR-29a	13.4	11.7	13.5	15.0			0.0
  hsa-miR-29c	11.2	8.7	12.3	13.3	1.5	2.6	0.0
  hsa-miR-301	6.0	9.6	7.7	7.9	0.0	0.0
  hsa-miR-30a-3p		8.2
  hsa-miR-30a-5p	11.9	12.6	11.3	13.0		2.0
  hsa-miR-30b	11.7	12.6	11.2	13.0		2.8
  hsa-miR-30c	13.0	13.9	12.7	14.3		4.5
  hsa-miR-30d	10.8	11.9	10.1	12.1	3.8	0.0
  hsa-miR-30e-3p	8.3	9.0	6.8	9.9		0.0
  hsa-miR-30e-5p	12.5	8.1	10.2	14.8			0.0
  hsa-miR-31				9.0
  hsa-miR-32	8.4		7.0	10.5
  hsa-miR-320	10.1	8.2	10.3	10.5	1.5	2.6	0.0
  hsa-miR-324-3p	8.8	9.9	9.0	9.5
  hsa-miR-324-5p	8.0			7.8
  hsa-miR-328	8.1	8.6	7.4	8.7		2.8
  hsa-miR-331	9.4	12.6	12.0	10.8	0.0		2.6
  hsa-miR-335	6.3			6.9
  hsa-miR-339	7.3		5.7	8.9
  hsa-miR-342	12.7	12.5	10.6	13.1		0.0
  hsa-miR-345	6.8	7.1	7.8	8.6			3.4
  hsa-miR-361				8.3
  hsa-miR-365	6.0	7.8	8.3	8.4	0.0
  hsa-miR-374	8.0	8.9	10.4	9.8
  hsa-miR-423	8.0	9.7	7.6	8.7	0.0	0.0
  hsa-miR-425				6.9
  hsa-miR-425-5p	8.7	11.6	9.7	10.2	0.0	0.0
  hsa-miR-484	11.5	12.3	12.9	12.0
  hsa-miR-486	7.0	8.8	9.9	9.6	3.8
  hsa-miR-532	7.0			7.8
  hsa-miR-545			5.5	6.3
  hsa-miR-572		7.7		6.8
  hsa-miR-629	6.7	7.3	7.2	9.0			1.4
  hsa-miR-646		6.0		7.3
  hsa-miR-650		8.8	11.4	8.0
  hsa-miR-659		7.6	6.0	6.8
  hsa-miR-660	9.5		8.6	10.1
  hsa-miR-7	6.1			5.8
  hsa-miR-9		7.8	6.8	6.2		2.8
  hsa-miR-9*			6.4	6.5
  hsa-miR-92	14.5	15.8	14.2	15.5	3.6	0.0
  hsa-miR-93	11.5	14.6	10.5	12.3	0.0	0.0	0.0
  hsa-miR-98	6.3	5.0	5.0	7.8			2.1
  hsa-miR-99a				7.3
  hsa-miR-99b				6.8

• TABLE 5
  miRNAs measured using the multiplex RT-PCR assay, but not consistently
  detected in any B-cell population

  hsa-let-7e	hsa-miR-383	hsa-miR-376a	hsa-miR-566
  hsa-miR-I0a	hsa-miR-198	hsa-miR-376b	hsa-miR-551b
  hsa-miR-I0b	hsa-miR-224	hsa-miR-380-5p	hsa-miR-569
  hsa-miR-34a	hsa-miR-299-5p	hsa-miR-410	hsa-miR-570
  hsa-miR-34b	hsa-miR-409-5p	hsa-miR-412	hsa-miR-548a
  hsa-miR-34c	hsa-miR-432	hsa-miR-432	hsa-miR-586
  hsa-miR-107	hsa-miR-433	hsa-miR-512-5p	hsa-miR-587
  hsa-miR-181c	hsa-miR-485-5p	hsa-miR-199a	hsa-miR-548b
  hsa-miR-215	hsa-miR-489	hsa-miR-199b	hsa-miR-588
  hsa-miR-218	hsa-miR-494	hsa-miR-219	hsa-miR-589
  hsa-miR-372	hsa-miR-506	hsa-miR-323	hsa-miR-550
  hsa-miR-375	hsa-miR-508	hsa-miR-338	hsa-miR-591
  hsa-miR-378	hsa-miR-521	hsa-miR-368	hsa-miR-593
  hsa-miR-137	hsa-miR-134	hsa-miR-373	hsa-miR-596
  hsa-miR-200a	hsa-miR-147	hsa-miR-373	hsa-miR-597
  hsa-miR-I	hsa-miR-149	hsa-miR-382	hsa-miR-622
  hsa-miR-183	hsa-miR-153	hsa-miR-424	hsa-miR-599
  hsa-miR-302a	hsa-miR-187	hsa-miR-448	hsa-miR-600
  hsa-miR-302c	hsa-miR-190	hsa-miR-450	hsa-miR-624
  hsa-miR-302d	hsa-miR-193a	hsa-miR-451	hsa-miR-601
  hsa-miR-367	hsa-miR-196a	hsa-miR-452	hsa-miR-626
  hsa-miR-369-5p	hsa-miR-196b	hsa-miR-452	hsa-miR-548d
  hsa-miR-449	hsa-miR-205	hsa-miR-453	hsa-miR-639
  hsa-miR-497	hsa-miR-208	hsa-miR-485-3p	hsa-miR-613
  hsa-miR-501	hsa-miR-213	hsa-miR-488	hsa-miR-614
  hsa-miR-509	hsa-miR-220	hsa-miR-490	hsa-miR-615
  hsa-miR-510	hsa-miR-325	hsa-miR-492	hsa-miR-616
  hsa-miR-511	hsa-miR-326	hsa-miR-493	hsa-miR-548c
  hsa-miR-514	hsa-miR-337	hsa-miR-503	hsa-miR-617
  hsa-miR-515-3p	hsa-miR-340	hsa-miR-504	hsa-miR-642
  hsa-miR-515-5p	hsa-miR-380-3p	hsa-miR-505	hsa-miR-618
  hsa-miR-517a	hsa-miR-422b	hsa-miR-507	hsa-miR-644
  hsa-miR-517b	hsa-miR-422a	hsa-miR-513	hsa-miR-647
  hsa-miR-517c	hsa-miR-429	hsa-miR-516-5p	hsa-miR-649
  hsa-miR-518a	hsa-miR-491	hsa-miR-517	hsa-miR-661
  hsa-miR-518b	hsa-miR-496	hsa-miR-518c	hsa-miR-662
  hsa-miR-518c	hsa-miR-500	hsa-miR-518f	hsa-miR-449b
  hsa-miR-518d	hsa-miR-502	hsa-miR-519b	hsa-miR-653
  hsa-miR-518e	hsa-miR-105	hsa-miR-519c	hsa-miR-411
  hsa-miR-520a	hsa-miR-122a	hsa-miR-519d	hsa-miR-654
  hsa-miR-520b	hsa-miR-124a	hsa-miR-51ge	hsa-miR-575
  hsa-miR-520c	hsa-miR-126	hsa-miR-522	hsa-miR-576
  hsa-miR-520d	hsa-miR-128b	hsa-miR-523	hsa-miR-578
  hsa-miR-520e	hsa-miR-129	hsa-miR-524	hsa-miR-579
  hsa-miR-520f	hsa-miR-130a	hsa-miR-526b	hsa-miR-580
  hsa-miR-520g	hsa-miR-139	hsa-miR-96	hsa-miR-585
  hsa-miR-520h	hsa-miR-143	hsa-miR-651	hsa-miR-
  			512-3p
  hsa-miR-95	hsa-miR-145	hsa-miR-376a	hsa-miR-631
  hsa-miR-126	hsa-miR-182	hsa-miR-542-5p	hsa-miR-363
  hsa-miR-127	hsa-miR-185	hsa-miR-544	hsa-miR-487b
  hsa-miR-133a	hsa-miR-189	hsa-miR-656	hsa-miR-645
  hsa-miR-135a	hsa-miR-18b	hsa-miR-549	hsa-miR-556
  hsa-miR-135b	hsa-miR-202	hsa-miR-657	hsa-miR-558
  hsa-miR-184	hsa-miR-202	hsa-miR-658	hsa-miR-627
  hsa-miR-204	hsa-miR-299-3p	hsa-miR-652	hsa-miR-630
  hsa-miR-206	hsa-miR-302a	hsa-miR-551a	hsa-miR-603
  hsa-miR-211	hsa-miR-302b	hsa-miR-552	hsa-miR-606
  hsa-miR-216	hsa-miR-302b	hsa-miR-553	hsa-miR-607
  hsa-miR-217	hsa-miR-302c	hsa-miR-554	hsa-miR-608
  hsa-miR-330	hsa-miR-329	hsa-miR-555	hsa-miR-609
  hsa-miR-371	hsa-miR-33	hsa-miR-562	hsa-miR-633
  hsa-miR-379	hsa-miR-362	hsa-miR-563	hsa-miR-565*
  hsa-miR-381	hsa-miR-369-3p	hsa-miR-564	hsa-miR-594*
  miRNAs marked with * were not used in analyses because they have been reclassified as non-miRNAs.

• The B cell subsets were profiled for gene expression at the whole genome level, as described previously. See Dave et al. N Engl J Med. 2004; 351:2159-2169. At each stage, we identified differentially expressed genes as those genes with a mean two-fold difference in expression and a false-discovery rate of less than 1%. See FIGS. 1E, 1G, and 1I. Genes that we found to be differentially expressed in each stage-transition were consistent with previous studies that examined gene expression in B cell subsets using microarrays with fewer probes, an overlap that was found to be highly statistically significant (P<0.001, chi-squared test). See, e.g., Klein et al. Proc Natl Acad Sci USA. 2003; 100:2639-2644; and Shaffer et al. Immunity. 2001; 15: 375-385.
• In the naïve→germinal center (GC) B cell transition, we identified 32 miRNAs that were differentially expressed. Interestingly, all but 4 miRNAs were found to be expressed more highly in GC cells than in naive B cells. See FIG. 1D. We confirmed the mRNA expression patterns of several genes that are known to be differentially expressed in the transition including BCL6, MME, MYBL1, as well as LM02. See FIG. 1E. LM02 was found to be expressed more highly in germinal center B cells compared to both naive B cells and memory B cells. See FIGS. 1E and 1I. In the GC→plasma cell transition, we found 33 miRNAs that were differentially expressed. Once again, we noted a striking asymmetry, with all but 2 miRNAs found to be expressed highly in GC cells, but down-regulated in plasma cells. See FIG. 1F. We also confirmed that the plasma cell-specific genes, PRDM1 (FIG. 1G), XBP1 and IRF4 were highly differentially expressed in our experiments. In the GC→memory B cell transition, there was a preponderance of the 27 significant miRNAs expressed at higher levels in memory cells, See FIG. 1H. Five miRNAs were expressed highly in GC cells compared to all the other B cell types. These included 3 members of the miR-17˜92 cluster (miR-17-5p, miR-20b, miR-93), as well as miR-28 and miR-181b.
• The expression pattern of all the miRNAs that were measurable in at least one of the B cell subsets is summarized in FIG. 5. Notably, there were no differences in the expression of genes involved in miRNA processing, including DICER1, DROSHA, XP05 (exportin5), EIF2C2 (ag02) and DGCR8, among the B cell subsets. See FIG. 1J.
• Separately, we examined the expression of predicted target genes of differentially expressed microRNAs. We also found that predicted mRNA target genes of microRNAs expressed highly in GC cells were expressed at lower levels in GC cells compared to other stages. See FIG. 6 and Appendix A. FIG. 6A shows density plots of the expression frequency of predicted mRNA targets of microRNAs expressed highly in germinal center B cells compared to naive cells. mRNAs from FIG. 1E that were also predicted targets of the miRNAs (N=830) were plotted for both naive cells and germinal center B cells. The depicted p-value was calculated using a 1-sided Kolmogorov-Smirnov test. FIG. 6B shows density plots of the expression frequency of predicted mRNA targets of miRNAs expressed highly in germinal center B cells compared to plasma cells. mRNAs from FIG. 1G that were also predicted targets of the miRNAs (N=1098) were plotted for both plasma cells and germinal center B cells. The depicted p-value was calculated using a 1-sided Kolmogorov-Smirnov test. FIG. 6C shows density plots of the expression frequency of predicted mRNA targets of miRNAs expressed highly in the germinal center B cells compared to memory B cells. mRNAs from FIG. 1I that were also predicted targets of the miRNAs (N=269) were plotted for both naive cells and germinal center B cells. The depicted p-value was calculated using a 1-sided Kolmogorov-Smirnov test.
• In the naïve to germinal center transition (see FIG. 6A), genes with at least a two-fold change in expression were analyzed by SAM using a false discovery rate of less than 1% (Larsson et. al, BMC Bioinformatics. 2005; 6:129), depicted in FIG. 1E. From that list of significantly differentially expressed genes, we determined those that were predicted targets of miRNAs (defined as genes with 3′UTR sequence complementarity to microRNA nucleotides 2-7) that were expressed more highly in germinal center cells. The gene expression distribution of those mRNA targets was graphed for naïve B Cells (blue curve) and germinal center B cells (orange curve). Thus, in FIG. 6A, we show genes differentially expressed between naïve and germinal center B cells that are also predicted targets of miRNA expressed more highly in germinal center B cells. We observe that the expression of these microRNA target genes is lower in germinal center B cells compared to naïve B cells.
• A similar analysis was carried out for the transitions from germinal center to plasma cell (FIG. 6B) and germinal center to memory cell (FIG. 6C). In all three cases examined, the distribution of germinal center miRNA target gene expression is statistically significantly lower (leftward shift of orange curve), which suggests a possible gene regulatory role for the miRNAs that are more highly expressed in germinal center cells. We note that the observed lower expression of the miRNA target genes could, however, also be caused by other factors such as downstream effects of particular transcription factors. The complete set of genes plotted in FIG. 6 is listed in Appendix A.
• Finally, we found that a higher proportion of differentially expressed transcription factors are predicted microRNA targets. See FIG. 7. Table 6 shows differentially expressed transcription factors, with the average level of expression for each in naïve, germinal center, plasma, and memory cells. Transcription factors for which the significance analysis showed a q value of 0 are indicated.

• TABLE 6
  Differentially-expressed transcription factors

  						Naïve		GC
  			Germinal	Plasma		vs	GC	vs
  		Naïve	Center	Cell	Memory	GC: q	vs PC: q	Mem: q
  ID	NAME	average	average	average	average	value	value	value

  1	AHR	8.9	10.33	8.36	10.38	0	0
  2	APBB2	6.86	7.87	7.35	6.39	0		0
  3	ARID1A	12.3	12.22	11.11	12.17		0
  4	ARID3A	8.03	6.9	9.81	8.76	0	0	0
  5	ARNTL	9.47	8.4	8.23	9.7	0		0
  6	ATF7	7.83	8.97	8.23	8.44	0
  7	BACH1	10.68	9.97	11.76	10.59		0
  8	BHLHB3	11.11	8.05	13.26	13.26	0	0	0
  9	BPTF	12.23	12.46	11.34	11.86		0
  10	BTG1	15.88	14.95	13.94	15.28		0
  11	CASP8AP2	10.17	10.55	9.44	10.22		0
  12	CBL	11.07	11.69	10.26	11.04		0
  13	CBX4	12.23	12.36	13.69	12.68		0
  14	CCNE1	7.86	9.18	8.87	7.66	0		0
  15	CDH1	5.85	3.85	10.26	4.91		0
  16	CITED2	9.11	9.88	12.56	9.22		0
  17	CLOCK	9.85	7.68	9.91	9.62	0	0	0
  18	CREB5	4.91	5.46	7.24	6.95		0
  19	CREBL1	7.53	6.97	8.06	8.09			0
  20	CREBL2	10.18	9.95	11.1	10.79		0
  21	CSDA	12.12	8.59	10.1	8.98	0	0
  22	DEK	12.73	14.09	10.29	13.15	0	0
  23	DLX2	6.08	4.66	7.04	7.08		0	0
  24	DR1	11.83	11.97	10.65	12.1		0
  25	DTX1	10.98	11.6	10.55	9.62		0	0
  26	DYRK1B	6.32	5.92	7.61	4.96		0
  27	E2F1	7.01	8.37	7.95	7.21	0		0
  28	EGR1	11.92	13.24	13.77	12.75	0
  29	EGR2	10.21	10.43	8.07	10.4		0
  30	EGR3	11.59	9.35	8.24	10.62	0	0	0
  31	ELF1	12.39	12.7	11.34	12.55		0
  32	ELL	7.12	7.83	9.17	7.59		0
  33	ELL2	9.25	8.23	13.6	9.27		0
  34	EPAS1	7.1	7.02	8.39	7.5		0
  35	ETS1	14.25	14.4	12.62	14.17		0
  36	ETS2	6.37	7.5	5.66	4.9	0	0	0
  37	ETV1	8.19	6.9	7.38	7.06	0
  38	ETV4	5.99	5.42	7.15	6.09		0
  39	ETV6	10.36	7.14	8.89	10.43	0	0	0
  40	FHL2	4.82	7.77	6.82	5.65	0		0
  41	FLNA	9.95	9.2	8.57	11.03			0
  42	FOS	12.35	11.25	13.34	11.23	0	0
  43	FOSB	10.13	7.21	11.05	10.79	0	0	0
  44	FOXC1	6.81	5.84	7.39	6.59		0
  45	FOXF2	7.05	4.83	6.37	5.84	0
  46	FOXJ2	10.01	8.38	9.97	9.85	0	0	0
  47	FOXK2	8.94	8.4	9.63	8.71		0
  48	GATA3	8.05	7.76	8.51	9.6			0
  49	GATA6	7.08	5.41	7.59	6.26	0	0
  50	GCN5L2	10.18	8.97	8.9	10.36	0		0
  51	GLI2	6.93	7.26	7.52	6.09			0
  52	GPX3	6.72	5.92	7.61	5.58		0
  53	HCLS1	13.94	14.42	13.12	13.92		0
  54	HIPK2	9.36	8.66	11.04	10.85		0	0
  55	HMGA1	11.26	12.47	10.36	10.86	0	0	0
  56	HMGB1	15.47	16.36	14.98	14.97		0	0
  57	HMGB2	12.96	15.78	12.73	13.36	0	0	0
  58	HOXA1	5.36	7.7	7.23	7.64	0
  59	HOXA5	7.03	8.32	8.36	7.54	0
  60	HOXB4	8.2	7.58	9.27	8.01		0
  61	HOXB9	7.4	6.2	8.41	6.87		0
  62	HOXC11	6.6	6.4	7.88	6.21		0
  63	HOXC8	7.05	5.48	6.88	6.71	0
  64	ID4	6.49	5.93	7.79	6.41		0
  65	ILF3	11.26	11.94	10.71	10.99		0
  66	IRAK1	12.19	11.48	12.8	11.96		0
  67	IRF4	11.16	9.28	13.66	10.51	0	0	0
  68	ISL1	6.98	5.91	7.6	6.11		0
  69	JAZF1	11.93	10.65	7.49	11.31	0	0
  70	JMJD1C	12.34	12.34	11.11	12.56		0
  71	JUN	12.14	9.34	12.7	12.16	0	0	0
  72	JUNB	11.62	9.72	10.84	11.37	0		0
  73	KLF11	8.08	5.94	6.88	8.39	0		0
  74	KLF2	12.61	9.1	11.7	12.61	0	0	0
  75	KLF4	10.04	7.57	9.09	7.49	0	0
  76	KLF5	7.08	5.26	6.14	5.07	0
  77	KLF6	12.06	11.33	9.24	12.40		0	0
  78	KLF7	9.36	8.35	8.88	9.8			0
  79	KLF9	9.47	7.34	6.64	9.27	0		0
  80	LITAF	12.52	10.18	11.82	12.49	0	0	0
  81	LMO1	6.86	4.75	7.16	6.88		0
  82	MAF	8.05	9.95	8.25	10.83	0	0
  83	MAFB	6.58	7.3	8.56	7.29		0
  84	MAML3	8.05	10.85	9.07	7.04	0	0	0
  85	MDFIC	12.06	13.67	13.6	13.08	0
  86	MEIS2	7.45	7.15	7.56	5.29			0
  87	MEN1	10.79	11.33	10.49	10.23			0
  88	MITF	7.51	8.05	8.01	6.41			0
  89	MTF1	10.08	9.92	8.86	10.5		0
  90	MXD1	9.07	7.59	9.62	8.9	0	0	0
  91	MXI1	10.63	7.85	11.1	9.42	0	0	0
  92	MYB	6.97	8.62	5.61	7.99	0	0
  93	MYBL1	9.68	14.13	10.02	9.72	0	0	0
  94	MYBL2	9.07	12.41	9.12	8.52	0	0	0
  95	NAT14	6.62	7.66	7.33	7.63	0
  96	NF1	9.23	10.46	11.21	9.88	0
  97	NFAT5	11.71	12.09	10.72	11.77		0
  98	NFATC4	6.36	7.92	8.96	5.71	0		0
  99	NFIX	8.09	7.43	8.67	7.37		0
  100	NFKB1	11.91	11.9	10.7	11.55		0
  101	NFYC	10.2	10.27	9.03	10.03		0
  102	NR3C2	8.85	5.58	4.91	8.41	0		0
  103	NR6A1	9.1	6.03	6.42	7.62	0		0
  104	NRIP1	11.79	10.3	10.33	11.20	0
  105	PAX3	7.01	5.55	5.01	5.96	0
  106	PHF1	11.85	10.69	12.26	11.45	0	0
  107	POU3F1	6.08	7.52	6.86	7.52	0
  108	POU4F1	5.62	8.85	8.46	7.53	0		0
  109	POU4F2	7.09	5.72	7.21	5.1	0	0
  110	PPARD	11.23	9.98	10.29	10.69	0
  111	PPARG	7.79	7.22	6.74	5.47			0
  112	PPARGC1B	6.89	6.09	6.51	7.91			0
  113	PRDM1	8.49	9.15	14.1	9.33		0
  114	PRDM4	10.48	9.81	9.76	10.83			0
  115	PROX1	7.18	6.38	7.89	5.54		0
  116	RAN	13.46	14.57	12.39	13.78	0	0
  117	RSF1	10.05	9.1	9.56	10.18			0
  118	RUNX1T1	7.37	5.95	6.63	6.34	0
  119	RUNX2	8.75	7.91	10.22	9.75		0	0
  120	RUNX3	11.8	11.13	10.26	12.47			0
  121	RXRA	9.07	7.15	8.81	8.32	0	0	0
  122	SAP30	8.22	9.91	9.28	8.33	0		0
  123	SCMH1	8.48	9.69	9.65	8.62	0		0
  124	SCML1	10.32	8.48	7.81	7.91	0
  125	SCML2	10.33	8.76	9.27	8.96	0
  126	SF1	11.72	11.44	10.36	11.37		0
  127	SIAH2	10.35	12.34	10.57	9.90	0	0	0
  128	SLC2A4RG	8.25	8.98	7.4	8.91		0
  129	SMAD1	5.91	8.1	6.34	7.33	0	0
  130	SMAD2	10.45	11.57	10.8	10.98	0
  131	SMAD3	10.72	9.23	7.74	10.54	0	0	0
  132	SMARCA2	11.14	10.12	10.79	11.19	0		0
  133	SOLH	8.78	7.88	8.7	9.03			0
  134	SOX4	8.99	9.14	9.17	10.2			0
  135	SOX5	9.3	10.47	7.33	9.08	0	0	0
  136	SOX9	6.72	8.42	8.18	7.03	0		0
  137	SP4	11.26	10.14	9.58	11.75	0		0
  138	SRCAP	8.58	8.06	6.96	8.38		0
  139	SREBF1	8.23	8.36	6.56	5.89		0	0
  140	STAT5B	10.35	10.95	9.73	10.7		0
  141	SUFU	7.12	6.03	7.84	7.62		0	0
  142	SUPT16H	11.12	11.35	9.6	10.71		0
  143	SUPT3H	7.77	6.3	8.05	8.35	0	0	0
  144	TARDBP	12.31	12.72	11.49	11.91		0
  145	TBP	10.55	10.14	9.09	10.38		0
  146	TBX3	5.67	6.05	7.89	5.53		0
  147	TCEA1	13.31	15	14.05	13.6	0		0
  148	TCERG1	12.29	12.5	11.46	12.04		0
  149	TCF7	10.21	10.82	9.97	12.61			0
  150	TFAP2A	6.7	5.54	8.26	6.88		0
  151	TFAP4	7.28	5.29	5.42	5.13	0
  152	TFDP1	10.95	12.9	10.49	11.36	0	0	0
  153	TFEB	11.56	11.15	9.49	11.77		0
  154	THRA	7.41	7.26	8.48	7.05		0
  155	THRB	7.45	7.51	6.11	6.29		0	0
  156	TLE1	9.19	7.74	9.63	9.13	0	0	0
  157	TMF1	10.76	9.68	10.71	10.42	0
  158	TSC22D3	13.22	11.26	12.67	13.4	0	0	0
  159	UHRF1	9.23	12.22	8.72	8.96	0	0	0
  160	VEZF1	10.98	11.45	9.94	10.81		0
  161	XBP1	10.1	10.74	15.84	10.65		0
  162	YBX1	14.79	15.31	14.2	14.53		0
  163	YWHAH	8.94	10.82	9.09	9.36	0	0	0
  164	YWHAZ	13.38	13.62	12.61	13.62		0
  165	ZFP36L1	14.07	12.89	10.09	13.87	0	0
  166	ZHX3	8.29	7.76	7.47	6.66			0
  167	ZNF207	12.29	13.51	12.69	12.54	0
  168	ZNF217	11.68	10.77	9.73	11.61		0
  169	ZNF219	6.13	6.11	8.42	6.48		0
  170	ZNF238	13.05	11.27	10.08	12.54	0	0	0
  171	ZNF3	8.86	7.27	8.75	8.89	0	0	0
  172	ZNF367	7.97	10.99	8.04	8.22	0	0	0
  173	ZNF398	10.39	9.28	10.47	10.25	0	0

Example 3 MiR-223 Regulates LM02 in the Naive˜Germinal Center and Germinal Center˜Memory Cell Transitions
• MiR-223 has previously been described as being important in the commitment to myeloid lineage. See Chen et al. Science. 2004; 303:83-86; Johnnidis et al. Nature. 2008; 451:1125-1129. We found miR-223 to be expressed at nearly 8-fold higher levels in both naive and memory cells compared to GC cells. This miRNA has a highly conserved sequence complementarity to the 3 ′UTR of 2 transcription factors that are expressed highly during GC cell differentiation: LM02 (FIG. 2A) and MYBL1 (FIG. 8A). Higher expression of miR-223 in the naive B cell stage could inhibit the untimely expression of these transcription factors until the cell is ready to undergo the germinal center reaction.
• We evaluated the effects of miR-223 expression on its predicted target gene, LM02, by transfecting precursors of miR-223 into a cell line derived from GC cell lymphoma cells (B1AB). Over-expression of miR-223 resulted in a consistent down-regulation of LM02 at the transcript level compared to a transfection with a scrambled control with no sequence complementarity to the human genome. See FIG. 2B. In that figure, the blue bars depict expression of LM02 24 hours after transfection with a scrambled control that does not possess complementarity to the human genome. The orange bars depict the expression of LM02 24 hours after transfection with a precursor for miR-223. The expression of LM02 was consistently lower in the cells treated with the miR-223 precursor, and the results were statistically significant (P<0.05 in all cases, student's t-test). There was no effect on the expression of a non-target control, beta-2 micro globulin in these experiments (data not shown). Over-expression of miR-223 also resulted in a consistent down-regulation of LM02 at the protein level compared to a transfection with a scrambled control with no sequence complementarity to the human genome. See FIG. 2C. We quantified the results of 3 separate experiments examining LM02 protein expression and found consistent down-regulation of LM02 in cells treated with miR-223 compared to cells transfected with scrambled controls. See FIG. 2D. Those results that were statistically significant (P<0.05, student's t-test). The extent of down-regulation of LM02 mRNA and protein by miR-223 was comparable, suggesting that miR-223 regulation of LM02 occurs predominantly at the mRNA level. Similarly, over-expression of miR-223 resulted in a down-regulation of MYBL1 transcripts. See FIG. 8B. In that figure, the blue bars depict expression of MYBL1 mRNA 24 hours after transfection with a scrambled control that does not possess complementarity to the human genome. The orange bars depict the expression of Mybl1 24 hours after transfection with a precursor for miR-223. The expression of LM02 was consistently lower in the cells treated with the miR-223 precursor (P<0.05 in all cases).
• As additional validation, we investigated whether the miR-223 had a direct effect on LM02 by cloning the 3′UTR sequence of LM02 3′ to the firefly luciferase ORF (Fluc). See Gottwein et al. Nature. 2007; 450:1096-1099. The resulting constructs and the unmodified vector were co-transfected into 293T cells along with a Renilla luciferase internal control and pL-CMV-eGFP constructs expressing either no miRNA or miR-223. Fluc expression from constructs bearing LM02 3′ UTR sequences were differentially down-regulated by miR-223 compared to those with mutated seed sequences; the seed sequence mutant construct had consistently diminished miR-223 repression compared to the wild-type construct in 5 separate experiments. See FIG. 2E. Those results were statistically significant (P<0.05, student's t-test). These observations provide evidence for an inhibitory role for miR-223 in regulating the transcription factor LM02.
• Activation of LM02 has been associated with the development of leukemia in patients undergoing gene therapy. Hacein-Bey-Abina et al. Science. 2003; 302:415-419. On the other hand, higher expression of PRDM1 alone is sufficient to induce plasma cell-differentiation. Turner et al. Cell. 1994; 77:297-306. Inappropriate expression of such genes must be effectively turned off for a cell to maintain its state. This mode of regulation is reflected in the effects of miR-223, miR-9 and miR-30, which turn off the inappropriate expression of LM02 and PRDM1 and might promote state maintenance and inhibition of lymphomagenesis.
• On the other hand, our data also identify a number of instances in which miRNAs are co-expressed with their predicted targets. It is possible that such interactions within the cell help to stabilize a defined expression level by dampening fluctuations. For example, in GC cells, we found that miR-181b was strongly co-expressed with its predicted target, BCL6. Such interactions could also be important in B cell stage maintenance and curbing the oncogenic potential of genes involved in B cell differentiation. See Cattoretti et al. Cancer Cell. 2005; 7:445-455; Dorsett et al. Immunity. 2008; 28:630-638.
Example 4 MiR-9 and the miR-30 Family Regulate PRDM1 (blimp-1) in the Germinal Center→Plasma Cell Transition
• In the GC→plasma cell transition, we observed that several members of the miR-30 family were expressed at 2-fold or higher levels in GC cells. See FIG. 1F. The transcription factor PRDM1 is an essential regulator of plasma cell differentiation. Martins et al. Annu Rev Immunol. 2008; 26:133-169. The miR-30 family comprises 5 members (miR-30a, 30b, 30c, 30d and 30e), of which 4 (all except 30e) were found to be expressed at higher levels in GC cells compared to plasma cells. Control transfection experiments documented good specificity of the RT-PCR probes for individual members of the miR-30 family with no discernible cross-hybridization. See FIG. 9. Three separate transfection experiments using high concentrations of miR-30b precursors are shown in the left panel with measurement of miR-30b and miR-30d. Similar experiments were performed with precursors for miR-30d (right panel).
• The 3′UTR of PRDM1 contains 3 highly conserved binding sites complementary to the seed sequence of members of the miR-30 family, as well as 3 binding sites for the seed sequence of miR-9, 2 of which are highly conserved across multiple species. See FIG. 3A. The 3′UTR region of PRDM1 complementary to miR-30 is shown in red. The 3 sites are complementary to nucleotides 2-8 (UTR position 408), nucleotides 1-8 (UTR position 2370) and nucleotides 2-8 (UTR position 2383) on the miRNA, respectively. The 3′UTR region of PRDM1 complementary to miR-9 is shown in green. The 3 sites are complementary to nucleotides 1-7 (UTR position 1459), nucleotides 2-8 (UTR position 2108) and nucleotides 2-8 (UTR position 2323) on the miRNA respectively. These sites are highly conserved across a number of species, with the exception of one miR-9 site (UTR position 1459) that is only present in humans.
• To evaluate the effects of the miR-30 family and miR-9 on PRDM1 expression in plasma cells, we introduced precursors of miR-9, miR-30b and miR-30d into the U266 multiple myeloma (plasma cell) cell line. Overexpression of miR-30 family members miR-30b and miR-30d, as well as miR-9, had no effect on PRDM1 at the mRNA level. See FIG. 3B. In that figure, the blue bars depict expression of PRDM1 24 hours after transfection with a scrambled control with no complementarity to the human genome. The magenta bars depict the expression of PRDM1 24 hours after transfection with a hairpin precursor for miR-30b, while the red bars depict the expression of PRDM1 24 hours after transfection with a hairpin precursor for miR-30d. The green bars depict the expression of PRDM1 24 hours after transfection with a hairpin precursor for miR-9. By contrast, there was a consistent down-regulation of PRDM1 at the protein level. See FIGS. 3C and 3D. Those results were statistically significant in each case (P<0.05, student's t-test), except for the transfections with the precursor to miR-9 (P=0.08, student's t-test). Overexpression of each of those microRNAs had an average knockdown effect of around 40%. These exclusively post-transcriptional effects of miR-9 and miR-30 on PRDM1 expression are consistent with one mechanism of miRNA regulation that has been described previously in other systems. See, e.g., O'Donnell et al. Nature. 2005; 435:839-843; Gottwein et al. Nature. 2007; 450:1096-1099; Selbach et al. Nature. 2008; 455:58-63; and Back et al. Nature. 2008; 455:64-71. There was no effect on the expression of a non-target control (Actin).
• Additionally, luciferase reporter activity of the PRDM1 3′UTR construct was decreased by overexpression of miR-9, miR-30b, and miR-30d, but not their respective seed sequence mutants. See FIG. 3E, which shows the average of three experiments. The down-regulation of the luciferase reporter signal and its restoration in the mutant constructs was found to be statistically significant in each of the 3 microRNAs: miR-9, miR-30b and miR-30d (P<0.05, student's t-test). The luciferase reporter activity level was rescued to the activity level of the empty vector control when the seed sequence of the microRNAs was mutated.
• The combined effect of 5 different microRNA species (miR-30a, miR-30b, miR-30c, miR-30d and miR-9) is likely to be more potent than that of a single microRNA. The role of mutual repression of BCL6 and PRDM1 in the germinal center to plasma cell differentiation as been described previously. See Martins et al. Immunol. 2008; 26:133-169. Our data suggest that microRNAs may bolster the effects of BCL6 in the inhibition of PRDM1.
• Our data show that members of the miR-17˜92 family are consistently expressed in GC cells and may play a role in mature B cell differentiation. Interestingly, the miR-17˜92 family has been implicated in early B cell differentiation and mice lacking the loci that encode these miRNAs have arrested early B cell development. See Ventura et al. Cell. 2008; 132:875-886. The expression patterns of the miR-17˜92 family suggest that the regulatory motifs embedded in the interaction of this miRNA family and its targets might have an additional function in regulating mature B cell differentiation.
• A striking observation in this study is the high degree of asymmetry observed in relative expression of miRNAs in GC cells compared to naive and plasma cells. At least 2 hypotheses could account for these findings. First, miRNA expression may promote a highly regulated state that enables GC cells to interact with T cells and antigen presenting cells, and to leave the GC cells poised for differentiation into memory or plasma cells. Second, miRNAs expressed highly in naive and plasma cells may be underrepresented in current miRNA libraries. Such libraries are often constructed from lymph nodes, which are typically enriched in GC cells. High throughput sequencing of sorted populations of B cells could reveal novel miRNAs that are highly expressed in those populations. Interestingly, a larger number of miRNAs were highly expressed in memory cells compared to GC cells. This observation might stem from the fact that memory cells are known to be heterogeneous (Sanz et al. Semin Immunol. 2008; 20:67-82) and standard methods used to select memory cells may capture a diverse group of memory subpopulations.
Example 5 MiRNAs and B Cell Malignancies
• To examine the expression of B cell stage-specific microRNAs in B cell malignancies, we undertook miRNA profiling of 75 tissue samples derived from normal lymph nodes (N=5) as well as patients with B cell malignancies including the molecular subsets of diffuse large B cell lymphoma (DLBCL; see Rosenwald et al. N Engl J Med. 2002; 346:1937-1947), germinal center B cell-like (GCB) DLBCL (N=20) and activated B cell-like (ABC) DLBCL (N=20), as well as cases of IgV mutated and unmutated chronic lymphocytic leukemia (N=20) and Burkitt lymphoma (N=10).
• We constructed a Bayesian predictor from miRNAs that distinguished normal memory B cells from GC cells. See FIG. 1H. We tested the predictor in the B cell malignancies derived from germinal center B cells (Burkitt lymphoma and GCB DLBCL) along with chronic lymphocytic leukemia, which is thought to arise from memory B cells. See Klein et al. J Exp Med. 2001; 194:1625-1638. Using leave one out cross-validation, we found that the predictor constructed from miRNAs expressed in normal B cells was able to correctly identify the B cell specific stage of the B cell malignancy type in over 95% of the cases. See FIG. 4A.
• An interesting aspect regarding the role of miRNAs in malignancies is their reported down-regulation in a number of malignancies compared to normal cells from the same lineage. See Lu et al. Nature. 2005; 435:834-838. To further examine this effect in B cell malignancies, we examined the expression of 113 miRNAs that we had identified in normal B cells. See Table 4, above. Of the 113 miRNAs, 103 were detected using the microarrays that we used to profile B cell malignancies and normal lymph nodes. We applied a 2-sided student's t-test to evaluate the relative expression of those 103 miRNAs in B cell malignancies (N=60) and normal lymph nodes (N=5). 34 miRNAs were differentially expressed (P<0.05) at higher levels in malignant cells and 9 miRNAs were expressed more highly in normal cells. 60 miRNAs were not differentially expressed. See FIG. 4B.
• As additional validation, we examined miRNA cloning frequencies for sequences cloned from normal and malignant B cells. See Landgraf et al. Cell. 2007; 129:1401-1414. MiRNAs for which a sequence was identified in at least 2 of the 3 available normal B cell samples were used in the analysis. We applied a 2-sided student's t-test (P<0.05) to compare the differential cloning frequency of the miRNAs between normal B cells (N=3) and a variety of mature B cell malignancy patient samples and cell lines (N=42). In all, we found 56 miRNAs that were consistently expressed in normal B cells. We found 13 of those 56 miRNAs were differentially expressed (P<0.05) between normal and malignant B cells, of which 12 miRNAs were expressed more highly in malignant cells and 1 miRNA was expressed more highly in normal cells. See FIG. 4C. In order to avoid effects from tumor-infiltrating non-malignant cells, we repeated the analysis with 20 chronic lymphocytic leukemia samples in the malignant group. The results were similar to those obtained with the larger set of malignancies (data not shown).
• These results demonstrate that miRNAs are not down-regulated in B cell malignancies compared to normal B cells and that normal B cell stage-specific miRNAs are maintained in B cell malignancies.
• MicroRNA profiling also revealed that each B cell malignancy type had a distinctive pattern of miRNA expression. See FIG. 4D. In order to evaluate the ability of miRNA profiles to distinguish different B cell malignancy types, we constructed Bayesian predictors from the most highly differentially expressed miRNAs for each pair-wise comparison. See Tables 7, 8, and 9.

• TABLE 7
  Predictor microRNAs that distinguish germinal center B-cell (GCB)
  DLBCL from Burkitt Lymphoma, activated B-cell (ABC) DLBCL, and
  chronic lymphocytic leukemia

  	GCBvsBL	GCBvsABC	GCBvsCLL
  	hsa-miR-146a	hsa-miR-142-3p	hsa-miR-126
  	hsa-miR-154	hsa-miR-16	hsa-miR-130a
  	hsa-miR-155	hsa-miR-184	hsa-miR-I0b
  	hsa-miR-184	hsa-miR-191	hsa-miR-154
  	hsa-miR-29b	hsa-miR-19a	hsa-miR-193b
  	hsa-miR-29c	hsa-miR-19b	hsa-miR-199a-3p
  	hsa-miR-363	hsa-miR-299-5p	hsa-miR-365
  	hsa-miR-503	hsa-miR-32	hsa-miR-99b
  	hsa-miR-519c-5p	hsa-miR-30e*	hsa-miR-143
  	hsa-miR-301a	hsa-miR-151-5p	hsa-miR-585
  	hsa-miR-152	hsa-miR-583	hsa-miR-193a-5p
  	hsa-miR-30b*	mghv-miR-MI-7-5p	hsa-miR-886-5p
  	hsa-miR-590-5p	hsa-miR-142-5p	hsa-miR-I00
  	hsa-miR-149*	hsa-miR-106b	hsa-miR-768-5p
  	hsa-miR-300	hsa-miR-30e	hsa-miR-145
  	hsa-miR-625*	hsa-miR-140-3p	hsa-miR-943
  	kshv-miR-K12-3	hsa-miR-20a	hsa-miR-371-5p
  	hsa-miR-28-5p	hsa-miR-526b*	hsa-miR-675
  	hsa-miR-25*	hsa-miR-28-5p	hsa-miR-150
  	ebv-miR-BHRFI-2	hsa-miR-30c	hsa-miR-181a
  	BL = Burkitt lymphoma,
  	ABC = activated B-cell DLBCL,
  	CLL = chronic lymphocytic lymphoma

• TABLE 8
  Predictor microRNAs that distinguish activated B-cell (ABC) DLBCL
  from Burkitt Lymphoma and chronic lymphocytic leukemia

  	ABCvsBL	ABCvsCLL
  	hsa-miR-182	hsa-miR-193b
  	hsa-miR-377	hsa-miR-99b
  	hsa-miR-660	hsa-miR-551a
  	hsa-miR-595	mghv-miR-MI-7-3p
  	ebv-miR-BARTI0	hsa-miR-585
  	hsa-miR-532-5p	hsa-miR-617
  	hsa-miR-200c*	hsa-miR-629*
  	hsa-miR-362-3p	hsa-miR-575
  	hsa-miR-455-3p	kshv-miR-K12-6-5p
  	hsa-miR-128	hsa-miR-193a-5p
  	hsa-miR-21*	hsa-miR-30e
  	hsa-miR-589	hsa-miR-296-3p
  	hsa-miR-135a*	hsa-miR-518b
  	hsa-miR-532-3p	hsa-miR-492
  	hsa-miR-548d-5p	hsa-miR-220c
  	hsa-miR-652	hsa-miR-326
  	hsa-miR-150*	hsa-miR-671-5p
  	hsa-miR-330-5p	hsa-miR-340*
  	hsa-miR-339-3p	hsa-miR-150
  	hsa-miR-502-3p	hsa-miR-193b*
  	BL = Burkitt lymphoma,
  	CLL = chronic lymphocytic lymphoma.

• TABLE 9
  Predictor microRNAs that distinguish Burkitt lymphoma from chronic
  lymphocytic leukemia
  BLvsCLL

  	hsa-miR-130b
  	hsa-miR-154
  	hsa-miR-155
  	hsa-miR-29b
  	hsa-miR-29c
  	hsa-miR-637
  	hsa-miR-658
  	hsa-miR-193a-5p
  	hsa-miR-886-5p
  	hsa-miR-768-5p
  	hsa-miR-101
  	hsa-miR-933
  	hsa-miR-371-5p
  	hsa-miR-675
  	hsa-miR-150
  	hsa-miR-874
  	hsa-miR-181a
  	hsa-miR-30c
  	ebv-miR-BHRFI-2
  	hsa-miR-628-3p
  	CLL = chronic lymphocytic lymphoma

• We tested the performance of the predictor using leave-one-out cross-validation applied to the predictors for Burkitt lymphoma, chronic lymphocytic leukemia, activated B-cell diffuse large B-cell lymphoma, and germinal center B-cell DLBCL. For a sample prediction to be correct, it had to be classified correctly in each pair-wise comparison with all remaining entities. We found it to be over 90% accurate in the identification of each entity. See FIG. 10.
• We next expanded the analysis to include follicular lymphoma and Hodgkin's lymphoma. We included the samples from the earlier study and we undertook miRNA profiling of the following additional samples: normal lymph nodes (N=5) and patients with germinal center B cell-like (GCB) DLBCL (N=10), activated B cell-like (ABC) DLBCL (N=10), chronic lymphocytic leukemia (N=10), Burkitt lymphoma (N=10), follicular lymphoma (N=10), and Hodgkin's lymphoma (N=9). As before, microRNA profiling revealed that each B cell malignancy type had a distinctive pattern of miRNA expression. See FIG. 11.
• From that data, we identified microRNAs that can be used to distinguish each of the six B cell malignancies analyzed from the other five. Lists of those microRNAs, and whether they are expressed in high levels or low levels relative to the other 5 B cell malignancies, are shown in Tables 10 through 13. In certain embodiments, each of those microRNAs could be used to distinguish one of the six B cell malignancies from the other five. High and low in the table refer to at least a 2 fold difference when compared to other lymphomas and benign lymph nodes.

• TABLE 10
  Predictor microRNAs that distinguish Burkitt lymphoma, germinal center B-cell
  (GCB) DLBCL, and follicular lymphoma from all other B cell malignancies in the study

  	BL vs		GCB vs		FL vs
  BL miRNA list	others	GCB miRNA list	others	FL miRNA list	others
  hsa-miR-106a	High	hsa-miR-93/mmu-miR-	High	hsa-miR-96/mmu-miR-	High
  hsa-miR-17/mmu-	High	93/rno-miR-93		96/rno-miR-96
  miR-17/rno-miR-17-		hsa-miR-886-3p	High	hsa-miR-138/mmu-miR-	High
  5p/rno-miR-17		hsa-miR-365/mmu-miR-	High	138/rno-miR-138
  hsa-miR-628-3p	High	365/rno-miR-365		hsa-miR-342-5p/mmu-	High
  hsa-miR-193a-5p	High	hsa-miR-378/mmu-miR-	High	miR-342-5p/rno-miR-342-
  hsa-miR-371-5p	High	378/rno-miR-378		5p
  hsa-miR-20a/mmu-	High	hsa-miR-320/mmu-miR-	High	hsa-miR-337-3p	High
  miR-20a/rno-miR-20a		320/rno-miR-320		hsa-miR-301a/mmu-miR-	High
  hsa-miR-23a/mmu-	Low	hsa-miR-103/mmu-miR-	High	301a/rno-miR-301a
  miR-23a/rno-miR-23a		103/rno-miR-103		hsa-miR-922	High
  hsa-miR-27a/mmu-	Low	hsa-miR-637	High	hsa-miR-744/mmu-miR-	High
  miR-27a/rno-miR-27a		hsa-miR-148a/mmu-	High	744
  hsa-miR-34b/mmu-	Low	miR-148a		hsa-miR-221*	High
  miR-34b-3p		hsa-miR-199a-3p/hsa-	High	hsa-miR-215	High
  hsa-miR-21/mmu-	Low	miR-199b-3p/mmu-miR-		hsa-miR-197/mmu-miR-	High
  miR-21/rno-miR-21		199a-3p/mmu-miR-		197
  hsa-miR-29a/mmu-	Low	199b/rno-miR-199a-3p		hsa-miR-92b/mmu-miR-	High
  miR-29a/rno-miR-29a		hsa-miR-126*/mmu-	High	92b/rno-miR-92b
  hsa-let-7b/mmu-let-	Low	miR-126-5p/rno-miR-		hsa-miR-218-2*/mmu-	High
  7b/rno-let-7b		126*		miR-218-2*/rno-miR-
  hsa-let-7f/mmu-let-	Low	kshv-miR-K12-6-3p	High	218*
  7f/rno-let-7f		hsa-miR-125a-5p/mmu-	High	hsa-miR-636	High
  hsa-miR-29b/mmu-	Low	miR-125a-5p/rno-miR-		hsa-miR-600	High
  miR-29b/rno-miR-		125a-5p		kshv-miR-K12-7	High
  29b		hsa-miR-551b/mmu-	Low	hsa-miR-194/mmu-miR-	High
  hsa-miR-549	Low	miR-551b/rno-miR-551b		194/rno-miR-194
  hsa-miR-374a	Low	hsa-miR-923	Low	hsa-miR-524-5p	High
  hsa-miR-513a-5p	Low			hsa-miR-22*/mmu-miR-	High
  hsa-miR-148a/mmu-	Low			22*/rno-miR-22*
  miR-148a				hsa-miR-34c-5p/mmu-	High
  hsa-miR-223/mmu-	Low			miR-34c/rno-miR-34c
  miR-223/rno-miR-223				hsa-miR-151-3p	High
  hsa-miR-138-	Low			hsa-miR-425/mmu-miR-	High
  1*/mmu-miR-				425/rno-miR-425
  138*/rno-miR-138*				ebv-miR-BART16	High
  hsa-miR-342-	Low			hsa-miR-20b*	High
  3p/mmu-miR-342-				hsa-miR-574-3p/mmu-	High
  3p/rno-miR-342-3p				miR-574-3p
  hsa-miR-146b-	Low			hsa-miR-155*	High
  5p/mmu-miR-				hsa-miR-374b*	High
  146b/rno-miR-146b				hsa-miR-497/mmu-miR-	High
  hsa-miR-195/mmu-	Low			497/rno-miR-497
  miR-195/rno-miR-195				hsa-miR-708/mmu-miR-	High
  hsa-miR-103/mmu-	Low			708/rno-miR-708
  miR-103/rno-miR-103				hsa-miR-92a/mmu-miR-	High
  hsa-miR-888*	Low			92a/rno-miR-92a
  hsa-miR-363/mmu-	Low			hsa-miR-361-3p	High
  miR-363/rno-miR-363				hsa-miR-513a-3p	High
  hsa-miR-140-	Low			hsa-miR-934	High
  3p/mmu-miR-				hsa-miR-363*/rno-miR-	High
  140*/rno-miR-140*				363*
  hsa-miR-191/mmu-	Low			mghv-miR-M1-3	High
  miR-191/rno-miR-191				hsa-miR-129*	High
  hsa-miR-768-5p	Low			hsa-miR-148b/mmu-miR-	High
  hsa-miR-222/mmu-	Low			148b/rno-miR-148b-3p
  miR-222/rno-miR-222				hsa-miR-493	High
  hsa-miR-668/mmu-	Low			hsa-miR-151-5p/mmu-	High
  miR-668				miR-151-5p/rno-miR-151
  hsa-miR-221/mmu-	Low			hsa-miR-146b-3p	High
  miR-221/rno-miR-221				hsa-miR-886-5p	High
  hsa-miR-24-1*/mmu-	Low			hsa-miR-331-3p/mmu-	High
  miR-24-1*/rno-miR-				miR-331-3p/rno-miR-331
  24-1*				hsa-miR-574-5p/mmu-	High
  hsa-miR-29c/mmu-	Low			miR-574-5p
  miR-29c/rno-miR-29c				hsa-miR-424	High
  hsa-miR-146a/mmu-	Low			hsa-miR-152/mmu-miR-	High
  miR-146a/rno-miR-				152/rno-miR-152
  146a				hsa-miR-302a/mmu-miR-	High
  hsa-miR-154/mmu-	Low			302a
  miR-154/rno-miR-154				hsa-miR-181a/mmu-miR-	High
  hsa-miR-215	Low			181a/rno-miR-181a
  hsa-miR-487b/mmu-	Low			hsa-miR-509-5p	High
  miR-487b/rno-miR-				hsa-let-7d/mmu-let-	High
  487b				7d/rno-let-7d
  hsa-miR-155	Low			hsa-miR-18b	High
  				hsa-miR-620	High
  				hsa-miR-18a/mmu-miR-	High
  				18a/rno-miR-18a
  				hsa-miR-298	High
  				hsa-miR-98/mmu-miR-	High
  				98/rno-miR-98
  				hsa-miR-576-3p	High
  				hsa-miR-374b/mmu-miR-	High
  				374/rno-miR-374
  				hsa-miR-32*	High
  				hsa-miR-302d*	High
  				ebv-miR-BART17-5p	High
  				hsa-miR-620	High
  				hsa-miR-665	High
  				hsa-miR-185/mmu-miR-	High
  				185/rno-miR-185
  				hsa-miR-34b/mmu-miR-	High
  				34b-3p
  				hsa-let-7e/mmu-let-	High
  				7e/rno-let-7e

• TABLE 11
  Predictor microRNAs that distinguish activated B-cell (ABC) DLBCL
  from all other B cell malignancies in the study

  		ABC vs
  	ABC miRNA list	others
  	hsa-miR-126/mmu-miR-	High
  	126-3p/rno-miR-126
  	hsa-miR-125b/mmu-miR-	High
  	125b-5p/rno-miR-125b-5p
  	hsa-miR-145/mmu-miR-	High
  	145/rno-miR-145
  	hsa-miR-22/mmu-miR-	High
  	22/rno-miR-22
  	hsa-miR-21/mmu-miR-	High
  	21/rno-miR-21
  	hsa-miR-199a-3p/hsa-miR-	High
  	199b-3p/mmu-miR-199a-
  	3p/mmu-miR-199b/rno-
  	miR-199a-3p
  	hsa-miR-24/mmu-miR-	High
  	24/rno-miR-24
  	hsa-miR-143/mmu-miR-	High
  	143/rno-miR-143
  	hsa-miR-23b/mmu-miR-	High
  	23b/rno-miR-23b
  	hsa-miR-23a/mmu-miR-	High
  	23a/rno-miR-23a
  	hsa-miR-142-3p/mmu-miR-	High
  	142-3p/rno-miR-142-3p
  	hsa-let-7a/mmu-let-7a/rno-	High
  	let-7a
  	hsa-miR-146b-5p/mmu-	High
  	miR-146b/rno-miR-146b
  	hsa-miR-27a/mmu-miR-	High
  	27a/rno-miR-27a
  	hsa-miR-30a/mmu-miR-	High
  	30a/rno-miR-30a
  	hsa-miR-27b/mmu-miR-	High
  	27b/rno-miR-27b
  	hsa-let-7c/mmu-let-7c/rno-	High
  	let-7c
  	hsa-miR-921	Low
  	ebv-miR-BHRF1-2	Low
  	hsa-miR-199a-5p/mmu-	Low
  	miR-199a-5p/rno-miR-
  	199a-5p
  	hsa-miR-768-5p	Low
  	hsa-miR-491-3p	Low
  	hsa-miR-185/mmu-miR-	Low
  	185/rno-miR-185
  	ebv-miR-BART17-5p	Low
  	hsa-miR-32*	Low
  	hsa-miR-335/mmu-miR-	Low
  	335-5p/rno-miR-335
  	hsa-miR-149*	Low
  	hsa-miR-576-3p	Low
  	hsa-miR-214/mmu-miR-	Low
  	214/rno-miR-214
  	hsa-miR-184/mmu-miR-	Low
  	184/rno-miR-184
  	hsa-miR-520d-5p	Low
  	hsa-miR-518c*	Low
  	hsa-miR-801/mmu-miR-801	Low
  	hsa-miR-298	Low
  	hsa-miR-634	Low
  	hsa-miR-583	Low
  	hsa-miR-187*	Low
  	hsa-miR-30d/mmu-miR-	Low
  	30d/rno-miR-30d
  	hsa-miR-129-5p/mmu-miR-	Low
  	129-5p/rno-miR-129
  	hsa-miR-300	Low
  	hsa-miR-620	Low
  	hsa-miR-	Low
  	130b*/mmu-miR-
  	130b*
  	hsa-miR-20b/mmu-	Low
  	miR-20b/rno-miR-
  	20b-5p
  	kshv-miR-K12-3	Low
  	hsa-miR-28-3p/rno-	Low
  	miR-28*
  	hsa-miR-557	Low
  	hsa-miR-150/mmu-	Low
  	miR-150/rno-miR-
  	150
  	hsa-miR-98/mmu-	Low
  	miR-98/rno-miR-98
  	hsa-miR-486-	Low
  	hsa-miR-518a-
  	5p/hsa-miR-527
  	hsa-miR-302d*	Low
  	hsa-miR-516a-5p	Low
  	hsa-miR-148b/mmu-	Low
  	miR-148b/rno-miR-
  	148b-3p
  	hsa-miR-25*	Low
  	hsa-miR-374b/mmu-	Low
  	miR-374/rno-miR-
  	374
  	hsa-miR-638	Low
  	hsa-miR-302a/mmu-	Low
  	miR-302a
  	hsa-miR-99b*/mmu-	Low
  	miR-99b*/rno-miR-
  	99b*
  	hsa-miR-29c*/mmu-	Low
  	miR-29c*/rno-miR-
  	29c*
  	hsa-miR-138/mmu-	Low
  	miR-138/rno-miR-
  	138
  	hsa-miR-766	Low
  	hsa-miR-488	Low
  	hsa-miR-498	Low
  	hsa-miR-339-	Low
  	5p/mmu-miR-339-
  	5p/rno-miR-339-5p
  	hsa-miR-193b*	Low
  	hsa-miR-299-	Low
  	5p/mmu-miR-
  	299*/rno-miR-299
  	ebv-miR-BART8*	Low
  	hsa-miR-107/mmu-	Low
  	miR-107/rno-miR-
  	107
  	hsa-miR-519e*	Low
  	hsa-miR-146b-3p	Low
  	hsa-miR-552	Low
  	hsa-miR-509-5p	Low
  	hsa-miR-574-	Low
  	5p/mmu-miR-574-
  	5p
  	hsa-miR-524-5p	Low
  	mghv-miR-M1-7-5p	Low
  	hsa-miR-659	Low
  	hcmv-miR-UL148D	Low
  	hsa-miR-92a/mmu-	Low
  	miR-92a/rno-miR-
  	92a
  	hsa-miR-	Low
  	30e*/mmu-miR-
  	30e*/rno-miR-
  	30e*
  	hsa-miR-	Low
  	183*/mmu-miR-
  	183*
  	hsa-miR-144*	Low
  	hsa-miR-574-	Low
  	3p/mmu-miR-574-
  	3p
  	hsa-miR-889	Low
  	hsa-miR-525-5p	Low
  	kshv-miR-K12-8	Low
  	hsa-miR-32/mmu-	Low
  	miR-32/rno-miR-
  	32
  	hsa-miR-938	Low
  	hsa-miR-198	Low
  	hsa-miR-	Low
  	186/mmu-miR-
  	186/rno-miR-186
  	hsa-miR-	Low
  	18a/mmu-miR-
  	18a/rno-miR-18a
  	hsa-miR-516b	Low
  	hsa-miR-625*	Low
  	hsa-miR-551b*	Low
  	hsa-miR-885-5p	Low
  	hsa-miR-891a	Low
  	hsa-miR-	Low
  	340/mmu-miR-
  	340-5p/rno-miR-
  	340-5p
  	hsa-let-7d/mmu-	Low
  	let-7d/rno-let-7d
  	hsa-miR-151-	Low
  	5p/mmu-miR-151-
  	5p/rno-miR-151
  	hsa-miR-18b	Low
  	ebv-miR-BHRF1-1	Low
  	hsa-miR-510	Low
  	hsa-miR-625	Low
  	mghv-miR-M1-8	Low
  	ebv-miR-BART19-	Low
  	3p
  	hsa-miR-147	Low
  	hsa-miR-28-	Low
  	5p/mmu-miR-
  	28/rno-miR-28
  	ebv-miR-BART13	Low
  	hsa-miR-25/mmu-	Low
  	miR-25/rno-miR-
  	25
  	hsa-miR-519d	Low
  	hsa-miR-361-	Low
  	5p/mmu-miR-
  	361/rno-miR-361
  	hsa-miR-331-	Low
  	3p/mmu-miR-331-
  	3p/rno-miR-331
  	hsa-miR-423-	Low
  	3p/mmu-miR-423-
  	3p/rno-miR-423
  	hsa-miR-93/mmu-	Low
  	miR-93/rno-miR-
  	93

• TABLE 12
  Predictor microRNAs that distinguish chronic lymphocytic leukemia
  from all other B cell malignancies in the study

  		CLL vs
  	CLL miRNA list	others
  	hsa-miR-	High
  	30e*/mmu-miR-
  	30e*/rno-miR-30e*
  	hsa-miR-32/mmu-	High
  	miR-32/rno-miR-32
  	hsa-let-7g/mmu-	High
  	let-7g
  	hsa-miR-186/mmu-	High
  	miR-186/rno-miR-
  	186
  	hsa-miR-140-	High
  	5p/mmu-miR-
  	140/rno-miR-140
  	hsa-miR-	High
  	196a*/mmu-miR-
  	196a*/rno-miR-
  	196a*
  	hsa-miR-	High
  	487b/mmu-miR-
  	487b/rno-miR-487b
  	hsa-miR-150/mmu-	High
  	miR-150/rno-miR-
  	150
  	hsa-miR-147	High
  	hsa-miR-486-	High
  	5p/mmu-miR-486
  	hsa-miR-144*	High
  	hsa-miR-154/mmu-	High
  	miR-154/rno-miR-
  	154
  	hsa-miR-28-	High
  	5p/mmu-miR-
  	28/rno-miR-28
  	hsa-miR-299-	High
  	5p/mmu-miR-
  	299*/rno-miR-299
  	hsa-miR-33a/mmu-	High
  	miR-33/rno-miR-33
  	hsa-miR-363/mmu-	High
  	miR-363/rno-miR-
  	363
  	hsa-miR-891a	High
  	hsa-miR-768-5p	High
  	hsa-miR-361-	High
  	5p/mmu-miR-
  	361/rno-miR-361
  	hsa-miR-519d	High
  	hsa-miR-335/mmu-	High
  	miR-335-5p/rno-
  	miR-335
  	hsa-miR-668/mmu-	High
  	miR-668
  	hsa-let-7f/mmu-let-	High
  	7f/rno-let-7f
  	hsa-miR-24-	High
  	1*/mmu-miR-24-
  	1*/rno-miR-24-1*
  	hsa-miR-223/mmu-	High
  	miR-223/rno-miR-
  	223
  	hsa-miR-140-	High
  	3p/mmu-miR-
  	140*/rno-miR-140*
  	hsa-miR-144/mmu-	High
  	miR-144/rno-miR-
  	144
  	hsa-miR-638	High
  	hsa-miR-30d/mmu-	High
  	miR-30d/rno-miR-
  	30d
  	hsa-miR-423-	High
  	3p/mmu-miR-423-
  	3p/rno-miR-423
  	hsa-miR-155	High
  	hsa-miR-101/mmu-	High
  	miR-101a/rno-miR-
  	101a
  	hsa-miR-20b/mmu-	High
  	miR-20b/rno-miR-
  	20b-5p
  	hsa-miR-374a	High
  	hsa-miR-25/mmu-	High
  	miR-25/rno-miR-25
  	hsa-miR-199a-	High
  	5p/mmu-miR-199a-
  	5p/rno-miR-199a-
  	5p
  	hsa-miR-649	High
  	hsa-miR-191/mmu-	High
  	miR-191/rno-miR-
  	191
  	hsa-miR-30e/mmu-	High
  	miR-30e/rno-miR-
  	30e
  	hsa-miR-107/mmu-	High
  	miR-107/rno-miR-
  	107
  	hsa-miR-93/mmu-	High
  	miR-93/rno-miR-93
  	hsa-miR-29c/mmu-	High
  	miR-29c/rno-miR-
  	29c
  	hsa-miR-541*	High
  	hsa-miR-888*	High
  	hsa-miR-549	High
  	hsa-miR-19a/mmu-	High
  	miR-19a/rno-miR-
  	19a
  	hsa-miR-342-	High
  	3p/mmu-miR-342-
  	3p/rno-miR-342-3p
  	hsa-miR-142-	High
  	5p/mmu-miR-142-
  	5p/rno-miR-142-5p
  	hsa-miR-801/mmu-	High
  	miR-801
  	hsa-let-7i/mmu-let-	High
  	7i/rno-let-7i
  	hsa-miR-26a/mmu-	High
  	miR-26a/rno-miR-
  	26a
  	hsa-miR-15a/mmu-	High
  	miR-15a
  	hsa-miR-195/mmu-	High
  	miR-195/rno-miR-
  	195
  	hsa-miR-	High
  	106b/mmu-miR-
  	106b/rno-miR-106b
  	hsa-miR-26b/mmu-	High
  	miR-26b/rno-miR-
  	26b
  	hsa-miR-15b/mmu-	High
  	miR-15b/rno-miR-
  	15b
  	hsa-miR-222/mmu-	High
  	miR-222/rno-miR-
  	222
  	hsa-miR-185/mmu-	High
  	miR-185/rno-miR-
  	185
  	hsa-miR-550	High
  	hsa-let-7e/mmu-	Low
  	let-7e/rno-let-7e
  	hsa-miR-24/mmu-	Low
  	miR-24/rno-miR-24
  	hsa-miR-30c-	Low
  	2*/mmu-miR-30c-
  	2*/rno-miR-30c-2*
  	hsa-miR-765	Low
  	mghv-miR-M1-4	Low
  	hsa-miR-933	Low
  	hsa-miR-620	Low
  	hsa-miR-30b*	Low
  	hsa-miR-658	Low
  	hsa-miR-10a/mmu-	Low
  	miR-10a/rno-miR-
  	10a-5p
  	hsa-miR-665	Low
  	hsa-miR-185*	Low
  	hsa-miR-503	Low
  	hsa-miR-126*/mmu-miR-	Low
  	126-5p/rno-miR-126*
  	hsa-miR-10b/mmu-miR-	Low
  	10b/rno-miR-10b
  	hsa-miR-628-3p	Low
  	hsa-miR-422a	Low
  	hsa-miR-193a-5p	Low
  	hsa-miR-143/mmu-miR-	Low
  	143/rno-miR-143
  	hsa-miR-371-5p	Low
  	hsa-miR-100/mmu-miR-	Low
  	100/rno-miR-100
  	hsa-miR-365/mmu-miR-	Low
  	365/rno-miR-365
  	hsa-miR-145/mmu-miR-	Low
  	145/rno-miR-145
  	kshv-miR-K12-6-3p	Low
  	ebv-miR-BART6-3p	Low
  	hsa-miR-220c	Low
  	hsa-miR-519c-5p/hsa-miR-	Low
  	519b-5p/hsa-miR-523*/hsa-
  	miR-518e*/hsa-miR-
  	522*/hsa-miR-519a*
  	hsa-miR-130a/mmu-miR-	Low
  	130a/rno-miR-130a
  	hsa-miR-424	Low
  	hsa-miR-483-5p	Low
  	hsa-miR-193b	Low
  	hsa-miR-637	Low
  	hsa-miR-920	Low
  	hsa-miR-488	Low
  	ebv-miR-BHRF1-2	Low
  	hsa-miR-526b	Low
  	hsa-miR-126/mmu-miR-126-	Low
  	3p/rno-miR-126
  	hsa-miR-943	Low
  	hsa-miR-199a-3p/hsa-miR-	Low
  	199b-3p/mmu-miR-199a-
  	3p/mmu-miR-199b/rno-miR-
  	199a-3p
  	mghv-miR-M1-3	Low
  	hsa-miR-934	Low
  	hsa-miR-886-5p	Low
  	hsa-miR-200b*/mmu-miR-	Low
  	200b*
  	hsa-miR-485-3p/mmu-miR-	Low
  	485*
  	hsa-miR-181a/mmu-miR-	Low
  	181a/rno-miR-181a
  	hsa-miR-125b/mmu-miR-	Low
  	125b-5p/rno-miR-125b-5p

• TABLE 13
  Predictor microRNAs that distinguish Hodgkin's lympoma from all
  other B cell malignancies in the study

  		HL vs
  	HL miRNA list	others
  	hsa-miR-338-	High
  	5p/mmu-miR-338-
  	5p/rno-miR-338*
  	hsa-miR-433/mmu-	High
  	miR-433/rno-miR-
  	433
  	hsa-miR-552	High
  	hsa-miR-202	High
  	hsa-miR-299-3p	High
  	hsa-miR-509-3-5p	High
  	hsa-miR-490-5p	High
  	hsa-miR-508-5p	High
  	hsa-miR-181a-2*	High
  	hsa-miR-663	High
  	hsa-miR-326/mmu-	High
  	miR-326/rno-miR-
  	326
  	hsa-miR-542-	High
  	3p/mmu-miR-542-
  	3p/rno-miR-542-3p
  	hsa-miR-492	High
  	hsa-miR-584	High
  	hsa-miR-654-5p	High
  	ebv-miR-BART20-3p	High
  	hsa-miR-542-	High
  	5p/mmu-miR-542-
  	5p/rno-miR-542-5p
  	ebv-miR-BART9*	High
  	hsa-miR-124/mmu-	High
  	miR-124/rno-miR-
  	124
  	hsa-miR-551a	High
  	hsa-miR-208a/mmu-	High
  	miR-208a/rno-miR-
  	208
  	hsa-miR-220b	High
  	hsa-miR-615-	High
  	3p/mmu-miR-615-3p
  	hsa-miR-	High
  	135a*/mmu-miR-
  	135a*
  	hiv1-miR-H1	High
  	hsa-miR-124*/mmu-	High
  	miR-124*/rno-miR-
  	124*
  	hsa-miR-502-5p	High
  	hsa-miR-92b*	High
  	hsa-miR-518a-3p	High
  	hsa-miR-377*	High
  	hsa-miR-125a-	High
  	3p/mmu-miR-125a-
  	3p/rno-miR-125a-3p
  	hsa-miR-30c-	High
  	1*/mmu-miR-30c-
  	1*/rno-miR-30c-1*
  	hsa-miR-650	High
  	hsa-miR-629	High
  	hsa-miR-296-	High
  	3p/mmu-miR-296-
  	3p/rno-miR-296
  	hsa-miR-425*/mmu-	High
  	miR-425*
  	hsa-miR-514	High
  	hsa-miR-519e	High
  	hsa-miR-938	High
  	hsa-miR-340*/mmu-	High
  	miR-340-3p/rno-
  	miR-340-3p
  	hsa-miR-657	High
  	hsa-miR-9*/mmu-	High
  	miR-9*/rno-miR-9*
  	ebv-miR-BART7*	High
  	hsa-miR-612	High
  	hsa-miR-640	High
  	hsa-miR-623	High
  	hsa-miR-99b*/mmu-	High
  	miR-99b*/rno-miR-
  	99b*
  	hsa-miR-645	High
  	hsa-miR-484/mmu-	High
  	miR-484/rno-miR-
  	484
  	hsa-miR-376a*	High
  	hsa-miR-345	High
  	hsa-miR-586	High
  	hsa-miR-622	High
  	hsa-miR-206/mmu-	High
  	miR-206/rno-miR-
  	206
  	hcmv-miR-US25-1*	High
  	hsa-miR-302c*	High
  	hsa-miR-	High
  	106b*/mmu-miR-
  	106b*/rno-miR-
  	106b*
  	hsa-miR-500	High
  	hsa-miR-890	High
  	hsa-miR-10a*/mmu-	High
  	miR-10a*/rno-miR-
  	10a-3p
  	kshv-miR-K12-1	High
  	hsa-miR-629*	High
  	hsa-miR-193b*	High
  	ebv-miR-BHRF1-3	High
  	hsa-miR-183/mmu-	High
  	miR-183/rno-miR-
  	183
  	hsa-let-7b*/mmu-	High
  	let-7b*/rno-let-7b*
  	hsa-miR-409-	High
  	5p/mmu-miR-409-
  	5p/rno-miR-409-5p
  	hsa-miR-585	High
  	hsa-miR-526b*	High
  	hsa-miR-337-3p	High
  	hsa-miR-212/mmu-	High
  	miR-212/rno-miR-
  	212
  	hsa-miR-548b-3p	High
  	hcmv-miR-UL112	High
  	hsa-miR-601	High
  	hsa-let-7d*/mmu-	High
  	let-7d*/rno-let-7d*
  	hsa-miR-181b/mmu-	High
  	miR-181b/rno-miR-
  	181b
  	hsa-miR-195*	High
  	kshv-miR-K12-5	High
  	hsa-miR-500*	High
  	hsa-miR-24-	High
  	2*/mmu-miR-24-
  	2*/rno-miR-24-2*
  	hsa-miR-382/mmu-	High
  	miR-382/rno-miR-
  	382
  	ebv-miR-BART8*	High
  	hsa-miR-125b-	High
  	2*/rno-miR-125b*
  	hsa-miR-194*	High
  	hsa-miR-297/mmu-	High
  	miR-297a
  	hsa-miR-610	High
  	hsa-miR-575	High
  	hsa-miR-21*	High
  	hsa-miR-936	High
  	kshv-miR-K12-6-5p	High
  	hsa-miR-553	High
  	hsa-miR-652/mmu-	High
  	miR-652/rno-miR-
  	652
  	hsa-miR-877/mmu-	High
  	miR-877/rno-miR-
  	877
  	hsa-miR-526a/hsa-	High
  	miR-520c-5p/hsa-
  	miR-518d-5p
  	hsa-miR-122*	High
  	hsa-miR-576-5p	High
  	mghv-miR-M1-6	High
  	hsa-miR-551b*	High
  	hsa-miR-125b-	High
  	1*/mmu-miR-125b-
  	3p/rno-miR-125b-
  	3p
  	hsa-miR-137/mmu-	High
  	miR-137/rno-miR-
  	137
  	ebv-miR-BART18-3p	High
  	hsa-miR-452	High
  	hsa-miR-23a*/rno-	High
  	miR-23a*
  	hsa-miR-617	High
  	hsa-miR-550*	High
  	hsa-miR-557	High
  	hsa-miR-331-	High
  	5p/mmu-miR-331-
  	5p
  	hsa-miR-296-	High
  	5p/mmu-miR-296-
  	5p/rno-miR-296*
  	mghv-miR-M1-2	High
  	ebv-miR-BART6-3p	High
  	hsa-miR-518b	High
  	hsa-miR-99b/mmu-	High
  	miR-99b/rno-miR-
  	99b
  	hsa-miR-525-5p	High
  	hsa-miR-589	High
  	hsa-miR-7-2*	High
  	hsa-miR-490-	High
  	3p/mmu-miR-490
  	hsa-miR-	High
  	150*/mmu-miR-
  	150*
  	hsa-miR-17*/rno-	High
  	miR-17-3p
  	hsa-miR-509-3p	High
  	ebv-miR-BHRF1-1	High
  	hsa-miR-	High
  	183*/mmu-miR-
  	183*
  	hsa-miR-635	High
  	hsa-miR-	High
  	130b/mmu-miR-
  	130b/rno-miR-130b
  	mghv-miR-M1-8	High
  	hsa-miR-887	High
  	hsa-miR-210/mmu-	High
  	miR-210/rno-miR-
  	210
  	hsa-miR-766	High
  	hsa-miR-671-	High
  	5p/mmu-miR-671-
  	5p
  	hsa-miR-659	High
  	hsa-miR-330-	High
  	5p/mmu-miR-
  	330/rno-miR-330
  	hsa-miR-323-	High
  	3p/mmu-miR-323-
  	3p/rno-miR-323
  	ebv-miR-BART13	High
  	ebv-miR-BART5	High
  	hsa-miR-602	High
  	hcmv-miR-UL148D	High
  	hsa-miR-373*	High
  	hsa-miR-526b	High
  	hsa-miR-328/mmu-	High
  	miR-328/rno-miR-
  	328
  	hsa-miR-874/mmu-	High
  	miR-874/rno-miR-
  	874
  	ebv-miR-BART19-3p	High
  	hsa-miR-595	High
  	hsa-miR-889	High
  	mghv-miR-M1-7-5p	High
  	hsa-miR-483-5p	High
  	hsa-miR-	High
  	487b/mmu-miR-
  	487b/rno-miR-487b
  	hsa-miR-675	High
  	hsa-miR-220c	High
  	mghv-miR-M1-7-3p	High
  	hsa-miR-485-	High
  	3p/mmu-miR-485*
  	hsa-miR-198	High
  	hsa-miR-483-3p	High
  	hcmv-miR-UL70-3p	High
  	hsa-miR-149/mmu-	High
  	miR-149
  	hsa-miR-516a-5p	High
  	hsa-miR-	High
  	145*/mmu-miR-
  	145*
  	hsa-miR-656	High
  	hsa-miR-502-3p	High
  	hsa-miR-29c*/mmu-miR-	High
  	29c*/rno-miR-29c*
  	hsa-miR-937	High
  	hsa-miR-515-5p	High
  	hsa-miR-153/mmu-miR-	High
  	153/rno-miR-153
  	hsa-miR-519e*	High
  	hsa-miR-128/mmu-miR-	High
  	128/rno-miR-128
  	hsa-miR-516b	High
  	hsa-miR-532-5p/mmu-miR-	High
  	532-5p/rno-miR-532-5p
  	kshv-miR-K12-8	High
  	hsa-miR-455-3p	High
  	hsa-miR-27a*/mmu-miR-	High
  	27a*/rno-miR-27a*
  	hsa-miR-510	High
  	hsa-miR-505/rno-miR-505	High
  	hsa-miR-187*	High
  	hsa-miR-498	High
  	hsa-miR-625	High
  	hsa-miR-129-5p/mmu-miR-	High
  	129-5p/rno-miR-129
  	ebv-miR-BHRF1-2	High
  	hsa-miR-143*	High
  	kshv-miR-K12-3	High
  	hsa-miR-660	High
  	hsa-miR-25*	High
  	hsa-miR-29a*/mmu-miR-	High
  	29a*/rno-miR-29a*
  	hsa-miR-422a	High
  	hsa-miR-518a-5p/hsa-miR-	High
  	527
  	hsa-miR-519c-5p/hsa-miR-	High
  	519b-5p/hsa-miR-
  	523*/hsa-miR-518e*/hsa-
  	miR-522*/hsa-miR-519a*
  	hsa-miR-28-3p/rno-miR-28*	High
  	hsa-miR-300	High
  	hsa-miR-130a/mmu-miR-	High
  	130a/rno-miR-130a
  	hsa-miR-583	High
  	hsa-miR-149*	High
  	hsa-miR-184/mmu-miR-	High
  	184/rno-miR-184
  	hsa-miR-625*	High
  	hsa-miR-99a/mmu-miR-	High
  	99a/rno-miR-99a
  	hsa-miR-199b-5p	High
  	hsa-miR-513a-5p	High
  	hsa-miR-494/mmu-miR-	High
  	494/rno-miR-494
  	mghv-miR-M1-4	High
  	hsa-miR-634	High
  	hsa-miR-923	High
  	hsa-miR-503	High
  	ebv-miR-BART2-3p	High
  	hsa-miR-520d-5p	High
  	hsa-miR-30b*	High
  	hsa-miR-30c-2*/mmu-miR-	High
  	30c-2*/rno-miR-30c-2*
  	hsa-miR-658	High
  	hsa-miR-921	High
  	hsa-miR-423-5p/mmu-miR-	High
  	423-5p
  	hsa-miR-933	High
  	hsa-miR-23b/mmu-miR-	Low
  	23b/rno-miR-23b
  	hsa-miR-27b/mmu-miR-	Low
  	27b/rno-miR-27b
  	hsa-miR-550	Low
  	hsa-let-7a/mmu-let-7a/rno-	Low
  	let-7a
  	hsa-miR-24/mmu-miR-	Low
  	24/rno-miR-24
  	hsa-miR-451/mmu-miR-	Low
  	451/rno-miR-451
  	hsa-miR-30a/mmu-miR-	Low
  	30a/rno-miR-30a
  	hsa-miR-20b/mmu-miR-	Low
  	20b/rno-miR-20b-5p
  	hsa-miR-26a/mmu-miR-	Low
  	26a/rno-miR-26a
  	hsa-miR-26b/mmu-miR-	Low
  	26b/rno-miR-26b
  	hsa-miR-101/mmu-miR-	Low
  	101a/rno-miR-101a
  	hsa-miR-106b/mmu-miR-	Low
  	106b/rno-miR-106b
  	hsa-miR-16/mmu-miR-	Low
  	16/rno-miR-16
  	hsa-miR-29b/mmu-miR-	Low
  	29b/rno-miR-29b
  	hsa-miR-768-3p	Low
  	hsa-miR-30e/mmu-miR-	Low
  	30e/rno-miR-30e
  	hsa-miR-106a	Low
  	hsa-miR-142-5p/mmu-miR-	Low
  	142-5p/rno-miR-142-5p
  	hsa-miR-144/mmu-miR-	Low
  	144/rno-miR-144
  	hsa-miR-17/mmu-miR-	Low
  	17/rno-miR-17-5p/rno-miR-
  	17
  	hsa-miR-15b/mmu-miR-	Low
  	15b/rno-miR-15b
  	hsa-miR-30c/mmu-miR-	Low
  	30c/rno-miR-30c
  	hsa-miR-142-3p/mmu-miR-	Low
  	142-3p/rno-miR-142-3p
  	hsa-miR-20a/mmu-miR-	Low
  	20a/rno-miR-20a
  	hsa-miR-30b/mmu-miR-	Low
  	30b/rno-miR-30b-5p
  	hsa-miR-19a/mmu-miR-	Low
  	19a/rno-miR-19a
  	hsa-miR-19b/mmu-miR-	Low
  	19b/rno-miR-19b

• From the data in Tables 10 through 13, we identified subsets of microRNAs that are sufficient to distinguish each of the six B cell malignancies from the other five. We selected only microRNAs that are expressed more highly in the selected B cell malignancy. Those microRNAs are listed in Tables 14 and 15. In certain embodiments, each of the microRNAs listed in Tables 14 and 15 can be used to distinguish one B cell malignancy from the other five.

• TABLE 14
  Predictor microRNAs that distinguish activated B-cell (ABC) DLBCL, germinal
  center B-cell like (GCB) DLBCL, and Burkitt lymphoma

  ABC High	GCB High	BL High
  hsa-miR-22/mmu-miR-22/rno-miR-	hsa-miR-93/mmu-miR-93/rno-miR-93	hsa-miR-628-
  22	hsa-miR-103/mmu-miR-103/rno-miR-103	3p
  hsa-miR-21/mmu-miR-21/rno-miR-	hsa-miR-320/mmu-miR-320/rno-miR-320
  21	hsa-miR-125a-5p/mmu-miR-125a-5p/rno-miR-
  hsa-miR-24/mmu-miR-24/rno-miR-	125a-5p
  24
  hsa-miR-23b/mmu-miR-23b/rno-
  miR-23b
  hsa-miR-23a/mmu-miR-23a/rno-
  miR-23a
  hsa-let-7a/mmu-let-7a/rno-let-7a
  hsa-let-7c/mmu-let-7c/rno-let-7c

• TABLE 15
  Predictor microRNAs that distinguish chromic lymphocytic leukemia, follicular
  lymphoma, and Hodgkin's lymphoma

  CLL High	FL High	HL High
  hsa-miR-32/mmu-miR-32/rno-	hsa-miR-152/mmu-miR-	hsa-miR-498
  miR-32	152/rno-miR-152	hsa-miR-525-5p
  hsa-miR-150/mmu-miR-150/rno-	hsa-miR-885-5p	hsa-miR-551b*
  miR-150	hsv1-miR-H1	hsa-miR-340*/mmu-miR-340-
  hsa-miR-140-5p/mmu-miR-	hsa-miR-548d-5p	3p/rno-miR-340-3p
  140/rno-miR-140	hsa-miR-488	hsa-miR-494/mmu-miR-494/rno-
  hsa-let-7g/mmu-let-7g	ebv-miR-BART16	miR-494
  hsa-miR-154/mmu-miR-154/rno-	hsa-miR-22*/mmu-miR-	hsa-miR-183*/mmu-miR-183*
  miR-154	22*/rno-miR-22*	hsa-miR-659
  hsa-miR-486-5p/mmu-miR-486	hsa-miR-513a-3p	hsa-miR-193b*
  hsa-miR-101/mmu-miR-	hsa-miR-708/mmu-miR-	hsa-miR-766
  101a/rno-miR-101a	708/rno-miR-708	hsa-miR-516a-5p
  hsa-miR-30e/mmu-miR-30e/rno-	hsa-miR-425/mmu-miR-	hsa-miR-125b-1*/mmu-miR-
  miR-30e	425/rno-miR-425	125b-3p/rno-miR-125b-3p
  hsa-miR-768-5p	hsa-miR-337-3p	ebv-miR-BART6-3p
  hsa-miR-363/mmu-miR-363/rno-	ebv-miR-BART17-5p	ebv-miR-BART8*
  miR-363	hsa-miR-221*	hsa-miR-509-3-5p
  hsa-miR-668/mmu-miR-668	hsa-miR-92b/mmu-miR-	hsa-miR-602
  hsa-miR-147	92b/rno-miR-92b	ebv-miR-BHRF1-1
  hsa-miR-196a*/mmu-miR-	hsa-miR-197/mmu-miR-197	mghv-miR-M1-2
  196a*/rno-miR-196a*	hsa-miR-32*	hsa-miR-145*/mmu-miR-145*
  hsa-miR-142-5p/mmu-miR-142-	hsa-miR-342-5p/mmu-miR-	hsa-miR-296-5p/mmu-miR-296-
  5p/rno-miR-142-5p	342-5p/rno-miR-342-5p	5p/rno-miR-296*
  hsa-miR-199a-5p/mmu-miR-	hsa-miR-524-5p	hsa-miR-17*/rno-miR-17-3p
  199a-5p/rno-miR-199a-5p	hsa-miR-34c-5p/mmu-miR-	hsa-miR-452
  hsa-miR-24-1*/mmu-miR-24-	34c/rno-miR-34c	hsa-miR-326/mmu-miR-326/rno-
  1*/rno-miR-24-1*	hsa-let-7e/mmu-let-7e/rno-let-	miR-326
  hsa-miR-891a	7e	hsa-miR-652/mmu-miR-652/rno-
  hsa-miR-550	hsa-miR-151-3p	miR-652
  hsa-miR-801/mmu-miR-801	hsa-miR-744/mmu-miR-744	hsa-miR-623
  hsa-miR-549	hsa-miR-574-3p/mmu-miR-	hsa-miR-194*
  hsa-miR-888*	574-3p	hsa-miR-125a-3p/mmu-miR-
  	hsa-miR-600	125a-3p/rno-miR-125a-3p
  	hsa-miR-20b*	hsa-miR-10a*/mmu-miR-
  	hsa-miR-194/mmu-miR-	10a*/rno-miR-10a-3p
  	194/rno-miR-194	hsa-miR-519e
  	hsa-miR-363*/rno-miR-363*	hsa-miR-502-5p
  	hsa-miR-155*	hsa-miR-124*/mmu-miR-
  	hsa-miR-34b/mmu-miR-34b-3p	124*/rno-miR-124*
  	hsa-miR-922	hsa-miR-345
  	hsa-miR-497/mmu-miR-	hsa-miR-584
  	497/rno-miR-497	hsa-miR-650
  	hsa-miR-493	hsa-miR-202
  	hsa-miR-138/mmu-miR-	hsa-miR-548b-3p
  	138/rno-miR-138	hsa-miR-492
  	hsa-miR-215	hsa-miR-135a*/mmu-miR-135a*
  	hsa-miR-302a/mmu-miR-302a	ebv-miR-BART20-3p
  	hsa-miR-96/mmu-miR-96/rno-	hsa-miR-586
  	miR-96	hsa-miR-338-5p/mmu-miR-338-
  	hsa-miR-218-2*/mmu-miR-	5p/rno-miR-338*
  	218-2*/rno-miR-218*	hsa-miR-92b*
  	kshv-miR-K12-7	hiv1-miR-H1
  	hsa-miR-301a/mmu-miR-	hsa-miR-508-5p
  	301a/rno-miR-301a	hsa-miR-542-5p/mmu-miR-542-
  		5p/rno-miR-542-5p
  		hsa-miR-490-5p
  		hsa-miR-663
  		hsa-miR-433/mmu-miR-433/rno-
  		miR-433

• Finally, we identified sets of microRNAs that can distinguish between each pair of B cell malignancies in the study. Lists of those microRNAs are shown in Appendix B, Tables 16 to 30. In certain embodiments, each of the listed microRNAs is sufficient to distinguish between the two B cell malignancies listed in the table description.
• The consistent expression of a number of miRNAs in a diverse set of B cell malignances suggests a role for miRNAs in the maintenance of tumor phenotype. Assays for stage-specific B cell markers such as BCL6, a marker for GC cells, are essential in the clinical diagnosis of B cell malignancies. Our data suggest that stage-specific biology in B cell malignancies is retained at the miRNA level. Recent work has demonstrated the utility of gene expression profiling in reliably distinguishing closely related B cell malignancies. See Hummel et al. N Engl J Med. 2006; 354:2419-2430; Dave et al. N Engl J Med. 2006; 354:2431-2442. However, clinical translation of gene expression profiling has proved to be difficult because of the need for freshly frozen tissue. Since intact miRNAs can be isolated from tissues preserved using standard methods (see, e.g., Doleshal et al. J Mol Diagn. 2008; 10:203-211; Xi et al. Rna. 2007; 13:1668-1674), diagnostic methods based upon miRNA profiles could be fairly easy to translate to clinical use.
• Interestingly, in contrast to a previous study (Lu et al. Nature 2005; 435:834-838), we did not note a decrease in the expression of the total number or overall expression levels of miRNAs in B cell malignancies compared to normal lymph nodes. Although B cell malignancies maintain the expression of a number of stage-specific miRNAs, their miRNA expression patterns are clearly deranged compared to normal lymph nodes. The consequences of altered miRNA expression in B cell tumors would be important to explore in future studies.
• In conclusion, our study demonstrates that mature B cell subsets have distinct patterns of microRNA expression, suggesting a role for miRNAs in B cell differentiation. We provide experimental evidence that transcription factors such as LM02 and PRDM1 are direct targets of differentially expressed miRNAs. B cell malignancies demonstrate a distinct pattern of miRNA expression that could be useful in distinguishing morphologically identical subtypes of these tumors. The conserved expression of stage-specific microRNAs in normal and malignant B cells suggests a role for microRNAs in the maintenance of the mature B cell phenotype.
Example 6 Differential Expression of miRNAs in ABC DLBCL Vs. GBC DLBCL Malignancies
• As discussed above, microRNAs have shown promise as biomarkers in a number of malignancies. Diffuse large B cell lymphoma (DLBCL) is the most common form of lymphoma and is known to comprise at least 2 molecularly distinct subgroups with different responses to standard therapy. These two distinct subgroups are typically identified as ABC DLBCL and GC (or GCB) DLBCL.
• However, the current methods used to distinguish GCB from non GCB DLBCL are limited fashion and can yield inconsistent results. While gene expression profiling remains the gold standard for distinguishing these 2 molecular subgroups it is not routinely performed in clinical laboratories. In an effort to expand and improve the existing analytical options we sought to develop microRNA-based assays. We created RNA libraries from 31 different samples and performed deep sequencing analysis to identify the present miRNAs.
• Small RNA Library Creation and Deep Sequencing
• Total RNA was extracted from the 31 samples using the phenol-chloroform method to preserve microRNAs, using Ambion reagents. Total RNA (typically 5 μg) from each sample was run on denaturing polyacrylamide-urea gels. The ˜17-25 nucleotide RNAs were excised from the gel, ligated to sequencing adaptors on both ends and reverse transcribed. The resulting cDNA library was PCR-amplified for 15 cycles and gel purified on 6% acrylamide gel. The gel-purified amplicon quality and quantity were analyzed on a 6% acrylamide gel relative to oligonucleotides of known concentration and size. 120 μl of 1-4 μM library were loaded on to the Illumina cluster station, where DNA molecules were attached to high-density universal adaptors in the flow cells and amplified. The DNA clusters generated via this process were sequenced with sequencing-by-synthesis technology, where successive high-resolution images of the four-color fluorescence excitation dependent on the base incorporated during each cycle were captured. Sequencing reads were generated for each of the 31 samples and base calls were rendered using Illumina software. All the primary sequencing data and gene expression data is publicly available through the GEO archive through accession GSE22898.
• The small RNA libraries from the 31 samples which were subjected to massively parallel, high-throughput sequencing using the Illumina platform generated a total of 328 million separate reads. Our approach to analyzing the sequences and discovering microRNAs broadly follows a previously described method (see, Zheng, Q., et al. GOEAST: a web-based software toolkit for Gene Ontology enrichment analysis, Nucleic acids Res. 2008; 36 (Web Server issue: W358-363). All bioinformatics analyses were performed using a cluster of 1024 Linux computer nodes. Preprocessing was carried out using locally written Shell and Perl scripts.
• From the raw sequences generated by high-throughput sequencing, the 3′- and 5′-adaptor sequences were trimmed. Low quality sequences were identified as those sequencing reads that contained stretches of consecutive identical bases or uncalled nucleotides (N) in the first 12 bases and sequencing reads shorter than 17 nucleotides. To minimize redundancy, reads were initially curtailed to the first 22 nucleotides and identical sequences were represented with a single fasta entry for analysis. Each unique sequence was mapped to the reference genome (Ensembl, build 50) and reads were filtered such that only perfect alignments (full length, 100% identity) were retained. Reads that aligned to more than five positions in the genome and reads that overlapped with the UCSC RNA genes were identified and excluded from microRNA analysis.
• Identification and Analysis of the Captured miRNA Sequences
• Sequences that occurred 20 or more times in at least one sample were consolidated and annotated for the 31 samples. Genomic loci that overlapped with microRNAs described in miRBase (version 13) were identified as known microRNAs (see Table 31). The remaining genomic loci were identified as encoding candidate novel microRNAs (see Table 32).
• The vast majority (96%) of the candidate novel microRNAs were found in more than one sample, with only a small minority of microRNAs were expressed exclusively in a specific B cell subset or malignancy. Many of the microRNAs that we identified in normal and malignant B cells were expressed at ten-fold or higher levels in these non B cell cases. These findings suggest that the microRNAs we have identified are broadly expressed and may have roles in a number of diverse tissue types.
• miRNA Profiling Using Real-Time PCR
• miRNA expression profiling was conducted using the Applied Biosystems 384-well multiplexed real-time PCR assay using 400 ng of total RNA. Eight reactions, each containing 50 ng of RNA and a multiplex looped primer pool with endogenous small nucleolar (sno)-RNA controls, were used to reverse-transcribe the miRNAs in parallel fashion (see Tables 33 and 34 for primers). The completed reactions were loaded onto the 384-well plate per manufacturer's instructions, and real-time PCR was run on the ABI 7900HT Prism instrument. For each 384-well plate, we used the automatically determined cycle-threshold (C_T) using the SDS 2.2.1 software (Applied Biosystems). Consistent with manufacturer recommendations, a C_T greater than 35 was treated as undetected. The probes deemed to be present were normalized to the average expression of a sno-RNA control. The expression values were calculated as 2^−ΔCT, then median centered to 500 and log 2-transformed.
• For further validation of the deep sequencing results, we selected candidate novel microRNAs (see Table 34) that were detectably measured in at the sequencing data from least one of four diffuse large B cell lymphoma (DLBCL) cases. Using stem-loop reverse transcription (Ashburner, M., et al., Nat Genet. (2000); 25(1):25-29) for quantitative PCR, we tested the expression of the candidate microRNAs in 101 primary tumors from patients with DLBCL and found that about 92% were detectably measured by real-time PCR in at least 10% of these cases, suggesting that real-time PCR reproducibly identifies microRNAs that are expressed in lymphomas. We also used real-time PCR to measure the expression of known microRNAs (see Table 33) in the same 101 samples and found that over 90% of these were also detected in at least 10% of the cases using real-time PCR. We found that six of the seven RT-PCR constructs that targeted RNA hairpins that had low probability of being a microRNA resulted in no detectable signal. These results suggest that our assays have high specificity for microRNAs and that the computational predictions based on our sequencing data correctly identified microRNAs.
• Differentiating ABC DLBCL Vs. GCB DLBCL Malignancies
• Gene expression profiling of patients with DLBCL has demonstrated that the tumors comprise at least two distinct diseases with different response rates to standard chemotherapy regimens (Chen, C., et al., Nucleic Acids Res. (2005); 33(20):e179). We hypothesized that microRNAs might be used to make this clinically important distinction for which gene expression profiling remains the gold standard. We performed gene expression profiling on 101 DLBCL cases and further subdivided these cases into the molecular subgroups.
• Tumor samples from 101 patients with diffuse large B cell lymphoma were obtained at the time of diagnosis and freshly frozen. These cases were profiled using Affymetrix Gene 1.0 ST arrays. The molecular subgroups were distinguished using a Bayesian approach described previously (Ambros, V., et al., RNA (2003);9(3):277-279).
• We found that 25 microRNAs with the highest t-statistic were equally efficacious as the gene expression profiling in differentiating the two groups of DLBCL with over 95% overlap between the classifications rendered by the two methods, using leave out one cross-validation (see Table 35). Interestingly, a subset of these 25 predictor microRNAs was candidate novel microRNAs, suggesting a biological and clinical relevance for these candidate novel microRNAs in DLBCL tumors.
• Our work provides an exhaustive identification of the microRNAs in normal and malignant B cells; that is a prerequisite to the delineation of their role. Further, we have developed a comprehensive framework that spans the identification of microRNAs from deep sequencing data to measuring their expression using real-time PCR and validating their expression in primary human tumors.
• It is also conceivable that some of the low-abundance microRNAs that we have identified in our study may be expressed at higher levels in other development stages or in other cell types. This notion is confirmed by our examination of the novel microRNAs in non B cell data. For instance, a number of the microRNAs that we discovered were also present at 10-fold or higher levels in cell lines derived from breast cancer and cervical cancer, suggesting that the microRNAs that we have discovered in B cells have broad biological significance.
• Deep Sequencing Reveals a Novel miRNA Cluster that Regulates the TGF-β Pathway
• Although microRNAs appear to be distributed throughout the genome, a number of microRNAs have been found in clusters such as miR-17-92 that are transcribed from a single primary transcript and cleaved into the individual microRNAs by the enzyme DROSHA. We found 2 separate clusters of candidate novel microRNAs on chromosome 9 and chromosome 14 (within the IgH locus), respectively. The first cluster was previously annotated as a hypothetical gene LOC100130622, and subsequently discarded from Refseq when no associated protein was identified. Our data demonstrate that this cluster (miR-2355), conserved only in primates, encodes 6 separate microRNAs: has-miR-2355a-1, has-miR-2356-1, has-miR-2355a-2, has-miR-2356-2, hsa-miR2355a-3, and hsa-miR-2355b (see Table 32 for sequence).
• In order to evaluate whether the microRNAs encoded in these clusters originate from the same primary transcript, we took KMS12 multiple myeloma cells which express these microRNAs and used siRNA to knock-down the expression of the microRNA processing enzyme Drosha. This enzyme acts at the first step of microRNA processing by cleaving microRNA precursors from the primary transcript. We found that decreased Drosha expression was associated with increased accumulation of primary transcripts of both the miR-17-92 cluster as well as the novel miR-2355 cluster. MicroRNAs from miR-2355 cluster were found to be expressed more highly in normal germinal center (GC) B cells compared to memory cells.
• The microRNAs of this cluster all share the same seed sequence, suggesting that they target the same genes. Among the computationally predicted targets of this microRNA cluster, we identified SMAD2 and SMAD3 which are well known mediators of the TGF-beta signaling pathway. We noted that gene expression of both SMAD2 and SMAD3 in our set of 101 DLBCLs were inversely correlated with this cluster (P<0.001, correlation test). Gene set enrichment analysis revealed that expression of the TGF-beta pathway in DLBCL samples varied inversely with the expression of the microRNA cluster, with a higher expression of the microRNA associated with a lower expression of the pathway (P<10⁻⁶), which has been noted as a factor in the biology of these tumors.

• TABLE 31
  miRNAs identified by deep sequencing analysis.

  		Putative
  		Mature/		miRBase	miRBase
  SEQ		Minor		Mature/	Mature/Minor	miRBase	SEQ
  ID	Mature Sequence	miRNA	miRBase	Minor	Accession	Mature/Minor	ID
  NO.	Captured	ID	ID	ID	Number	Sequence	NO.
  1	CUGCGCAAGCUACUGCCUUGCU	hsa-let-	hsa-let-	hsa-let-	MIMAT0004585	CUGCGCAAGCUACUGCCUUGCU	393
  		7i*	7i	7i*
  2	CCACGGAUGUUUGAGCAUGUGC	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0004516	ACGGAUGUUUGAGCAUGUGCUA	394
  		105-1*	105-1	105*
  3	CCACGGAUGUUUGAGCAUGUGC	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0004516	ACGGAUGUUUGAGCAUGUGCUA	395
  		105-2*	105-2	105*
  4	UACUGCAAUGUAAGCACUUCUU	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0004517	CUGCAAUGUAAGCACUUCUUAC	396
  		106a*	106a	106a*
  5	CAUUAUUACUUUUGGUACGCG	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0000444	CAUUAUUACUUUUGGUACGCG	397
  		126*	126	126*
  6	AAGCCCUUACCCCAAAAAGUAU	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0004548	AAGCCCUUACCCCAAAAAGUAU	398
  		129*	129-1	129*
  7	AAGCCCUUACCCCAAAAAGCAU	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0004605	AAGCCCUUACCCCAAAAAGCAU	399
  		129-2*	129-2	129-3p
  8	UCUACAGUGCACGUGUCUCCAG	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0000250	UCUACAGUGCACGUGUCUCCAG	400
  		139-5p	139	139-5p
  9	CUGGUACAGGCCUGGGGGACAG	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0004610	CUGGUACAGGCCUGGGGGACAG	401
  		150*	150	150*
  10	ACUGCAGUGAAGGCACUUGUAG	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0000071	ACUGCAGUGAAGGCACUUGUAG	402
  		17*	17	17*
  11	UGAAUUACCGAAGGGCCAUAA	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0004560	GUGAAUUACCGAAGGGCCAUAA	403
  		183*	183	183*
  12	AGGGGCUGGCUUUCCUCUGGUC	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0004611	AGGGGCUGGCUUUCCUCUGGUC	404
  		185*	185	185*
  13	ACUGCCCUAAGUGCUCCUUCUGG	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0002891	ACUGCCCUAAGUGCUCCUUCUGG	405
  		18a*	18a	18a*
  14	AACUGGCCUACAAAGUCCCAGU	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0000459	AACUGGCCUACAAAGUCCCAGU	406
  		193a-3p	193a	193a-3p
  15	CGGGGUUUUGAGGGCGAGAUGA	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0004767	CGGGGUUUUGAGGGCGAGAUGA	407
  		193b*	193b	193b*
  16	CCCAGUGUUCAGACUACCUGUUC	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0000231	CCCAGUGUUCAGACUACCUGUUC	408
  		199a-2*	199a-2	199a-5p
  17	UAGUUUUGCAUAGUUGCACUAC	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0004490	AGUUUUGCAUAGUUGCACUACA	409
  		19a*	19a	19a*
  18	AGUUUUGCAGGUUUGCAUCCAGC	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0004491	AGUUUUGCAGGUUUGCAUCCAGC	410
  		19b-1*	19b-1	19b-1*
  19	ACUGUAGUAUGGGCACUUCCAG	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0004752	ACUGUAGUAUGGGCACUUCCAG	411
  		20b*	20b	20b*
  20	ACCUGGCAUACAAUGUAGAUUU	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0004568	ACCUGGCAUACAAUGUAGAUUU	412
  		221*	221	221*
  21	GGGUUCCUGGCAUGCUGAUUU	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0004587	UGGGUUCCUGGCAUGCUGAUUU	413
  		23b*	23b	23b*
  22	AGGCGGAGACUUGGGCAAUUG	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0004498	AGGCGGAGACUUGGGCAAUUG	414
  		25*	25	25*
  23	AGAGCUUAGCUGAUUGGUGAAC	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0004588	AGAGCUUAGCUGAUUGGUGAAC	415
  		27b*	27b	27b*
  24	CUGGGAGGUGGAUGUUUACUUC	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0004589	CUGGGAGGUGGAUGUUUACUUC	416
  		30b*	30b	30b*
  25	CUGGGAGAGGGUUGUUUACUCC	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0004674	CUGGGAGAGGGUUGUUUACUCC	417
  		30c-1*	30c-1	30c-1*
  26	CUGGGAGAAGGCUGUUUACUCU	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0004550	CUGGGAGAAGGCUGUUUACUCU	418
  		30c-2*	30c-2	30c-2*
  27	CUUUCAGUCGGAUGUUUACAGC	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0000693	CUUUCAGUCGGAUGUUUACAGC	419
  		30e*	30e	30e*
  28	UCCCUGUCCUCCAGGAGCUCACG	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0000764	UCCCUGUCCUCCAGGAGCUCACG	420
  		339-5p	339	339-5p
  29	UCUCACACAGAAAUCGCACCCGU	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0000753	UCUCACACAGAAAUCGCACCCGU	421
  		342-3p	342	342-3p
  30	AAUCAGCAAGUAUACUGCCCUA	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0004557	CAAUCAGCAAGUAUACUGCCCU	422
  		34a*	34a	34a*
  31	UCCCCCAGGUGUGAUUCUGAUUU	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0004682	UCCCCCAGGUGUGAUUCUGAUUU	423
  		361-3p	361	361-3p
  32	AAUCCUUGGAACCUAGGUGUGAGU	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0000705	AAUCCUUGGAACCUAGGUGUGAGU	424
  		362-5p	362	362-5p
  33	AGGGACUUUCAGGGGCAGCUGU	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0009199	AGGGACUUUCAGGGGCAGCUGU	425
  		365-2*	365-2	365*
  34	CUUAUCAGAUUGUAUUGUAAUU	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0004688	CUUAUCAGAUUGUAUUGUAAUU	426
  		374a*	374a	374a*
  35	CUUAGCAGGUUGUAUUAUCAUU	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0004956	CUUAGCAGGUUGUAUUAUCAUU	427
  		374b*	374b	374b*
  36	AGGUUACCCGAGCAACUUUGCAU	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0001638	AGGUUACCCGAGCAACUUUGCAU	428
  		409-5p	409	409-5p
  37	CAAAACGUGAGGCGCUGCUAU	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0004749	CAAAACGUGAGGCGCUGCUAU	429
  		424*	424	424*
  38	UAUGUGCCUUUGGACUACAUCG	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0003150	UAUGUGCCUUUGGACUACAUCG	430
  		455-5p	455	455-5p
  39	GUCAUACACGGCUCUCCUCUCU	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0002176	GUCAUACACGGCUCUCCUCUCU	431
  		485-3p	485	485-3p
  40	UGUCUUACUCCCUCAGGCACAU	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0003257	UGUCUUACUCCCUCAGGCACAU	432
  		550-1*	550-1	550*
  41	UGUCUUACUCCCUCAGGCACAU	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0003257	UGUCUUACUCCCUCAGGCACAU	433
  		550-2*	550-2	550*
  42	GAAAUCAAGCGUGGGUGAGACC	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0004794	GAAAUCAAGCGUGGGUGAGACC	434
  		551b*	551b	551b*
  43	AUUCUAAUUUCUCCACGUCUUU	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0003241	AUUCUAAUUUCUCCACGUCUUU	435
  		576-5p	576	576-5p
  44	UCAGAACAAAUGCCGGUUCCCAGA	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0003256	UCAGAACAAAUGCCGGUUCCCAGA	436
  		589*	589	589*
  45	GAGCUUAUUCAUAAAAGUGCAG	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0003258	GAGCUUAUUCAUAAAAGUGCAG	437
  		590-5p	590	590-5p
  46	GGGGGUCCCCGGUGCUCGGAUC	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0004804	GGGGGUCCCCGGUGCUCGGAUC	438
  		615-5p	615	615-5p
  47	GACUAUAGAACUUUCCCCCUCA	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0004808	GACUAUAGAACUUUCCCCCUCA	439
  		625*	625	625*
  48	UGGUGGGCCGCAGAACAUGUGC	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0003330	UGGUGGGCCGCAGAACAUGUGC	440
  		654-5p	654	654-5p
  49	AGGAAGCCCUGGAGGGGCUGGAG	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0003880	AGGAAGCCCUGGAGGGGCUGGAG	441
  		671-5p	671	671-5p
  50	AACUAGACUGUGAGCUUCUAGA	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0004927	CAACUAGACUGUGAGCUUCUAG	442
  		708*	708	708*
  51	CAACAAAUCACAGUCUGCCAUA	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0004553	CAACAAAUCACAGUCUGCCAUA	443
  		7-1*	7-1	7-1*
  52	AUAAAGCUAGAUAACCGAAAGU	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0000442	AUAAAGCUAGAUAACCGAAAGU	444
  		9-1*	9-1	9*
  53	AUAAAGCUAGAUAACCGAAAGU	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0000442	AUAAAGCUAGAUAACCGAAAGU	445
  		9-2*	9-2	9*
  54	AGGGACGGGACGCGGUGCAGUG	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0004792	AGGGACGGGACGCGGUGCAGUG	446
  		92b*	92b	92b*
  55	AUAAAGCUAGAUAACCGAAAGU	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0000442	AUAAAGCUAGAUAACCGAAAGU	447
  		9-3*	9-3	9*
  56	UGAGGUAGUAGGUUGUAUAGUU	hsa-let-	hsa-let-	hsa-let-	MIMAT0000062	UGAGGUAGUAGGUUGUAUAGUU	448
  		7a-2	7a-2	7a
  57	UGAGGUAGUAGGUUGUAUAGUU	hsa-let-	hsa-let-	hsa-let-	MIMAT0000062	UGAGGUAGUAGGUUGUAUAGUU	449
  		7a-3	7a-3	7a
  58	UGAGGUAGUAGGUUGUAUGGUU	hsa-let-	hsa-let-	hsa-let-	MIMAT0000064	UGAGGUAGUAGGUUGUAUGGUU	450
  		7c	7c	7c
  59	UGAGGUAGGAGGUUGUAUAGUU	hsa-let-	hsa-let-	hsa-let-	MIMAT0000066	UGAGGUAGGAGGUUGUAUAGUU	451
  		7e	7e	7e
  60	UGAGGUAGUAGAUUGUAUAGUU	hsa-let-	hsa-let-	hsa-let-	MIMAT0000067	UGAGGUAGUAGAUUGUAUAGUU	452
  		7f-1	7f-1	7f
  61	UGAGGUAGUAGUUUGUACAGUU	hsa-let-	hsa-let-	hsa-let-	MIMAT0000414	UGAGGUAGUAGUUUGUACAGUU	453
  		7g	7g	7g
  62	UGAGGUAGUAGUUUGUGCUGUU	hsa-let-	hsa-let-	hsa-let-	MIMAT0000415	UGAGGUAGUAGUUUGUGCUGUU	454
  		7i	7i	7i
  63	AACCCGUAGAUCCGAACUUGUG	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0000098	AACCCGUAGAUCCGAACUUGUG	455
  		100	100	100
  64	UACAGUACUGUGAUAACUGAA	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0000099	UACAGUACUGUGAUAACUGAA	456
  		101-1	101-1	101
  65	GUACAGUACUGUGAUAACUGAA	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0000099	UACAGUACUGUGAUAACUGAA	457
  		101-2	101-2	101
  66	AGCAGCAUUGUACAGGGCUAUGA	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0000101	AGCAGCAUUGUACAGGGCUAUGA	458
  		103-1	103-1	103
  67	AGCAGCAUUGUACAGGGCUAUGA	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0000101	AGCAGCAUUGUACAGGGCUAUGA	4589
  		103-2	103-2	103
  68	AAAUGCUCAGACUCCUGUGGUG	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0000102	UCAAAUGCUCAGACUCCUGUGGU	460
  		105-1	105-1	105
  69	AAAUGCUCAGACUCCUGUGGUG	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0000102	UCAAAUGCUCAGACUCCUGUGGU	461
  		105-2	105-2	105
  70	AAAAGUGCUUACAGUGCAGGUAG	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0000103	AAAAGUGCUUACAGUGCAGGUAG	462
  		106a	106a	106a
  71	UAAAGUGCUGACAGUGCAGAU	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0000680	UAAAGUGCUGACAGUGCAGAU	463
  		106b	106b	106b
  72	AGCAGCAUUGUACAGGGCUAUCA	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0000104	AGCAGCAUUGUACAGGGCUAUCA	464
  		107	107	107
  73	UACCCUGUAGAUCCGAAUUUGUG	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0000253	UACCCUGUAGAUCCGAAUUUGUG	465
  		10a	10a	10a
  74	UACCCUGUAGAACCGAAUUUGUG	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0000254	UACCCUGUAGAACCGAAUUUGUG	466
  		10b	10b	10b
  75	UGGAAUGUAAAGAAGUAUGUAU	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0000416	UGGAAUGUAAAGAAGUAUGUAU	467
  		1-1	1-1	1
  76	UGGAAUGUAAAGAAGUAUGUAU	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0000416	UGGAAUGUAAAGAAGUAUGUAU	468
  		1-2	1-2	1
  77	UGGAGUGUGACAAUGGUGUUUG	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0000421	UGGAGUGUGACAAUGGUGUUUG	469
  		122	122	122
  78	UAAGGCACGCGGUGAAUGCC	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0000422	UAAGGCACGCGGUGAAUGCC	470
  		124-1	124-1	124
  79	UAAGGCACGCGGUGAAUGCC	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0000422	UAAGGCACGCGGUGAAUGCC	471
  		124-2	124-2	124
  80	UAAGGCACGCGGUGAAUGCC	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0000422	UAAGGCACGCGGUGAAUGCC	472
  		124-3	124-3	124
  81	ACCCGUCCCGUUCGUCCCCGGA	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0005899	ACCCGUCCCGUUCGUCCCCGGA	473
  		1247	1247	1247
  82	ACGGUGCUGGAUGUGGCCUUU	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0005902	ACGGUGCUGGAUGUGGCCUUU	474
  		1250	1250	1250
  83	AGAAGGAAAUUGAAUUCAUUUA	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0005944	AGAAGGAAAUUGAAUUCAUUUA	475
  		1252	1252	1252
  84	AGCCUGGAAGCUGGAGCCUGCAGU	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0005905	AGCCUGGAAGCUGGAGCCUGCAGU	476
  		1254	1254	1254
  85	AGGAUGAGCAAAGAAAGUAGAUU	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0005906	AGGAUGAGCAAAGAAAGUAGAUU	477
  		1255a	1255a	1255a
  86	AGGCAUUGACUUCUCACUAGCU	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0005907	AGGCAUUGACUUCUCACUAGCU	478
  		1256	1256	1256
  87	CGUACCGUGAGUAAUAAUGCG	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0000445	UCGUACCGUGAGUAAUAAUGCG	479
  		126	126	126
  88	AUGGGUGAAUUUGUAGAAGGAU	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0005914	AUGGGUGAAUUUGUAGAAGGAU	480
  		1262	1262	1262
  89	AUGGUACCCUGGCAUACUGAGU	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0005915	AUGGUACCCUGGCAUACUGAGU	481
  		1263	1263	1263
  90	CAGGAUGUGGUCAAGUGUUGUU	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0005918	CAGGAUGUGGUCAAGUGUUGUU	482
  		1265	1265	1265
  91	CCUCAGGGCUGUAGAACAGGGCU	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0005920	CCUCAGGGCUGUAGAACAGGGCU	483
  		1266	1266	1266
  92	CUGGACUGAGCCGUGCUACUGG	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0005923	CUGGACUGAGCCGUGCUACUGG	484
  		1269	1269	1269
  93	CUGGAGAUAUGGAAGAGCUGUGU	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0005924	CUGGAGAUAUGGAAGAGCUGUGU	485
  		1270	1270	1270
  94	CUUGGCACCUAGCAAGCACUCA	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0005796	CUUGGCACCUAGCAAGCACUCA	486
  		1271	1271	1271
  95	UCGGAUCCGUCUGAGCUUGGCU	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0000446	UCGGAUCCGUCUGAGCUUGGCU	487
  		127-3p	127	127-3p
  96	UACGUAGAUAUAUAUGUAUUUU	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0005933	UACGUAGAUAUAUAUGUAUUUU	488
  		1277	1277	1277
  97	UAGUACUGUGCAUAUCAUCUAU	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0005936	UAGUACUGUGCAUAUCAUCUAU	489
  		1278	1278	1278
  98	UCACAGUGAACCGGUCUCUUU	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0000424	UCACAGUGAACCGGUCUCUUU	490
  		128-1	128-1	128
  99	UCACAGUGAACCGGUCUCUUU	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0000424	UCACAGUGAACCGGUCUCUUU	491
  		128-2	128-2	128
  100	CUUUUUGCGGUCUGGGCUUGC	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0000242	CUUUUUGCGGUCUGGGCUUGC	492
  		129-1	129-1	129-5p
  101	CUUUUUGCGGUCUGGGCUUGC	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0000242	CUUUUUGCGGUCUGGGCUUGC	493
  		129-2	129-2	129-5p
  102	UGUGAGGUUGGCAUUGUUGUCU	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0005884	UGUGAGGUUGGCAUUGUUGUCU	494
  		1294	1294	1294
  103	UUAGGCCGCAGAUCUGGGUGA	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0005885	UUAGGCCGCAGAUCUGGGUGA	495
  		1295	1295	1295
  104	UUCAUUCGGCUGUCCAGAUGUA	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0005800	UUCAUUCGGCUGUCCAGAUGUA		496
  		1298	1298	1298
  105	UUGCAGCUGCCUGGGAGUGACUUC	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0005797	UUGCAGCUGCCUGGGAGUGACUUC	497
  		1301	1301	1301
  106	CGGUUUGAGGCUACAGUGAGAU	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0005892	UUUGAGGCUACAGUGAGAUGUG	498
  		1304	1304	1304
  107	ACGUUGGCUCUGGUGGUG	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0005950	ACGUUGGCUCUGGUGGUG	499
  		1306	1306	1306
  108	ACUCGGCGUGGCGUCGGUCGUG	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0005951	ACUCGGCGUGGCGUCGGUCGUG	500
  		1307	1307	1307
  109	CAGUGCAAUGUUAAAAGGGCAU	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0000425	CAGUGCAAUGUUAAAAGGGCAU	501
  		130a	130a	130a
  110	CAGUGCAAUGAUGAAAGGGCAU	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0000691	CAGUGCAAUGAUGAAAGGGCAU	502
  		130b	130b	130b
  111	UAACAGUCUACAGCCAUGGUCG	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0000426	UAACAGUCUACAGCCAUGGUCG	503
  		132	132	132
  112	ACCGUGGCUUUCGAUUGUUACU	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0004594	ACCGUGGCUUUCGAUUGUUACU	504
  		132*	132	132*
  113	UGUGACUGGUUGACCAGAGGGG	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0000447	UGUGACUGGUUGACCAGAGGGG	505
  		134	134	134
  114	UAUGGCUUUUCAUUCCUAUGUGA	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0000758	UAUGGCUUUUCAUUCCUAUGUGA	506
  		135b	135b	135b
  115	ACUCCAUUUGUUUUGAUGAUGGA	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0000448	ACUCCAUUUGUUUUGAUGAUGGA	507
  		136	136	136
  116	AGCUGGUGUUGUGAAUCAGGCCG	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0000430	AGCUGGUGUUGUGAAUCAGGCCG	508
  		138-1	138-1	138
  117	AGCUGGUGUUGUGAAUCAGGCCG	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0000430	AGCUGGUGUUGUGAAUCAGGCCG	509
  		138-2	138-2	138
  118	UGGAGACGCGGCCCUGUUGGAG	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0004552	GGAGACGCGGCCCUGUUGGAGU	510
  		139-3p	139	139-3p
  119	UACCACAGGGUAGAACCACGG	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0004597	UACCACAGGGUAGAACCACGG	511
  		140-3p	140	140-3p
  120	UAACACUGUCUGGUAAAGAUGG	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0000432	UAACACUGUCUGGUAAAGAUGG	512
  		141	141	141
  121	CCCAUAAAGUAGAAAGCACU	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0000433	CAUAAAGUAGAAAGCACUACU	513
  		142	142	142-5p
  122	UGAGAUGAAGCACUGUAGCUC	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0000435	UGAGAUGAAGCACUGUAGCUC	514
  		143	143	143
  123	GGAUAUCAUCAUAUACUGUAAG	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0004600	GGAUAUCAUCAUAUACUGUAAG	515
  		144	144	144*
  124	GUCCAGUUUUCCCAGGAAUCCCU	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0000437	GUCCAGUUUUCCCAGGAAUCCCU	516
  		145	145	145
  125	UGAGAACUGAAUUCCAUGGGUU	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0000449	UGAGAACUGAAUUCCAUGGGUU	517
  		146a	146a	146a
  126	UGAGAACUGAAUUCCAUAGGCU	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0002809	UGAGAACUGAAUUCCAUAGGCU	518
  		146b	146b	146b-5p
  127	UCAGUGCACUACAGAACUUUGU	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0000243	UCAGUGCACUACAGAACUUUGU	519
  		148a	148a	148a
  128	UCAGUGCAUCACAGAACUUUGU	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0000759	UCAGUGCAUCACAGAACUUUGU	520
  		148b	148b	148b
  129	UCUCCCAACCCUUGUACCAGUG	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0000451	UCUCCCAACCCUUGUACCAGUG	521
  		150	150	150
  130	CUAGACUGAAGCUCCUUGAGG	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0000757	CUAGACUGAAGCUCCUUGAGG	522
  		151-3p	151	151-3p
  131	UCAGUGCAUGACAGAACUUGG	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0000438	UCAGUGCAUGACAGAACUUGG	523
  		152	152	152
  132	UUAAUGCUAAUCGUGAUAGGGGU	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0000646	UUAAUGCUAAUCGUGAUAGGGGU	524
  		155	155	155
  133	UAGCAGCACAUAAUGGUUUGUG	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0000068	UAGCAGCACAUAAUGGUUUGUG	525
  		15a	15a	15a
  134	UAGCAGCACAUCAUGGUUUACA	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0000417	UAGCAGCACAUCAUGGUUUACA	526
  		15b	15b	15b
  135	UAGCAGCACGUAAAUAUUGGCG	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0000069	UAGCAGCACGUAAAUAUUGGCG	527
  		16-1	16-1	16
  136	UAGCAGCACGUAAAUAUUGGCG	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0000069	UAGCAGCACGUAAAUAUUGGCG	528
  		16-2	16-2	16
  137	CAAAGUGCUUACAGUGCAGGUAG	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0000070	CAAAGUGCUUACAGUGCAGGUAG	529
  		17	17	17
  138	AACAUUCAACGCUGUCGGUGAGU	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0000256	AACAUUCAACGCUGUCGGUGAGU	530
  		181a-1	181a-1	181a
  139	AACAUUCAACGCUGUCGGUGAGU	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0000256	AACAUUCAACGCUGUCGGUGAGU	531
  		181a-2	181a-2	181a
  140	AACAUUCAUUGCUGUCGGUGGGU	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0000257	AACAUUCAUUGCUGUCGGUGGGU	532
  		181b-1	181b-1	181b
  141	AACAUUCAUUGCUGUCGGUGGGU	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0000257	AACAUUCAUUGCUGUCGGUGGGU	533
  		181b-2	181b-2	181b
  142	AACAUUCAACCUGUCGGUGAGU	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0000258	AACAUUCAACCUGUCGGUGAGU	534
  		181c	181c	181c
  143	AACAUUCAUUGUUGUCGGUGGGU	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0002821	AACAUUCAUUGUUGUCGGUGGGU	535
  		181d	181d	181d
  144	UUUGGCAAUGGUAGAACUCACACU	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0000259	UUUGGCAAUGGUAGAACUCACACU	536
  		182	182	182
  145	UAUGGCACUGGUAGAAUUCACU	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0000261	UAUGGCACUGGUAGAAUUCACU	537
  		183	183	183
  146	UGGACGGAGAACUGAUAAGGGU	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0000454	UGGACGGAGAACUGAUAAGGGU	538
  		184	184	184
  147	UGGAGAGAAAGGCAGUUCCUGA	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0000455	UGGAGAGAAAGGCAGUUCCUGA	539
  		185	185	185
  148	CAAAGAAUUCUCCUUUUGGGCU	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0000456	CAAAGAAUUCUCCUUUUGGGCU	540
  		186	186	186
  149	CAUCCCUUGCAUGGUGGAGGG	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0000457	CAUCCCUUGCAUGGUGGAGGG	541
  		188-5p	188	188-5p
  150	UAAGGUGCAUCUAGUGCAGAUAG	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0000072	UAAGGUGCAUCUAGUGCAGAUAG	542
  		18a	18a	18a
  151	CAACGGAAUCCCAAAAGCAGCUG	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0000440	CAACGGAAUCCCAAAAGCAGCUG	543
  		191	191	191
  152	CUGACCUAUGAAUUGACAGCC	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0000222	CUGACCUAUGAAUUGACAGCC	544
  		192	192	192
  153	UGGGUCUUUGCGGGCGAGAUGA	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0004614	UGGGUCUUUGCGGGCGAGAUGA	545
  		193a-5p	193a	193a-5p
  154	AACUGGCCCUCAAAGUCCCGCU	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0002819	AACUGGCCCUCAAAGUCCCGCU	546
  		193b	193b	193b
  155	UGUAACAGCAACUCCAUGUGGA	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0000460	UGUAACAGCAACUCCAUGUGGA	547
  		194-1	194-1	194
  156	UAGCAGCACAGAAAUAUUGGC	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0000461	UAGCAGCACAGAAAUAUUGGC	548
  		195	195	195
  157	UAGGUAGUUUCAUGUUGUUGGG	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0000226	UAGGUAGUUUCAUGUUGUUGGG	549
  		196a-1	196a-1	196a
  158	UAGGUAGUUUCAUGUUGUUGGG	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0000226	UAGGUAGUUUCAUGUUGUUGGG	550
  		196a-2	196a-2	196a
  159	UAGGUAGUUUCCUGUUGUUGGG	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0001080	UAGGUAGUUUCCUGUUGUUGGG	551
  		196b	196b	196b
  160	UUCACCACCUUCUCCACCCAGC	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0000227	UUCACCACCUUCUCCACCCAGC	552
  		197	197	197
  161	ACAGUAGUCUGCACAUUGGUUA	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0000232	ACAGUAGUCUGCACAUUGGUUA	553
  		199a-1	199a-1	199a-3p
  162	ACAGUAGUCUGCACAUUGGUUA	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0000232	ACAGUAGUCUGCACAUUGGUUA	554
  		199a-2	199a-2	199a-3p
  163	ACAGUAGUCUGCACAUUGGUUA	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0004563	ACAGUAGUCUGCACAUUGGUUA	555
  		199b	199b	199b-3p
  164	UGUGCAAAUCUAUGCAAAACUGA	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0000073	UGUGCAAAUCUAUGCAAAACUGA	556
  		19a	19a	19a
  165	UGUGCAAAUCCAUGCAAAACUGA	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0000074	UGUGCAAAUCCAUGCAAAACUGA	557
  		19b-1	19b-1	19b
  166	UGUGCAAAUCCAUGCAAAACUGA	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0000074	UGUGCAAAUCCAUGCAAAACUGA	558
  		19b-2	19b-2	19b
  167	UAACACUGUCUGGUAACGAUGU	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0000682	UAACACUGUCUGGUAACGAUGU	559
  		200a	200a	200a
  168	UAAUACUGCCUGGUAAUGAUGA	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0000318	UAAUACUGCCUGGUAAUGAUGA	560
  		200b	200b	200b
  169	UAAUACUGCCGGGUAAUGAUGGA	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0000617	UAAUACUGCCGGGUAAUGAUGGA	561
  		200c	200c	200c
  170	UUCCUAUGCAUAUACUUCUUUG	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0002810	UUCCUAUGCAUAUACUUCUUUG	562
  		202*	202	202*
  171	GUGAAAUGUUUAGGACCACUAG	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0000264	GUGAAAUGUUUAGGACCACUAG	563
  		203	203	203
  172	UUCCCUUUGUCAUCCUAUGCCU	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0000265	UUCCCUUUGUCAUCCUAUGCCU	564
  		204	204	204
  173	UCCUUCAUUCCACCGGAGUCUG	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0000266	UCCUUCAUUCCACCGGAGUCUG	565
  		205	205	205
  174	UGGAAUGUAAGGAAGUGUGUGG	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0000462	UGGAAUGUAAGGAAGUGUGUGG	566
  		206	206	206
  175	UAAAGUGCUUAUAGUGCAGGUAG	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0000075	UAAAGUGCUUAUAGUGCAGGUAG	567
  		20a	20a	20a
  176	CAAAGUGCUCAUAGUGCAGGUAG	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0001413	CAAAGUGCUCAUAGUGCAGGUAG	568
  		20b	20b	20b
  177	UAGCUUAUCAGACUGAUGUUGA	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0000076	UAGCUUAUCAGACUGAUGUUGA	569
  		21	21	21
  178	CUGUGCGUGUGACAGCGGCUGA	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0000267	CUGUGCGUGUGACAGCGGCUGA	570
  		210	210	210
  179	UAACAGUCUCCAGUCACGGCC	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0000269	UAACAGUCUCCAGUCACGGCC	571
  		212	212	212
  180	ACAGCAGGCACAGACAGGCAGU	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0000271	ACAGCAGGCACAGACAGGCAGU	572
  		214	214	214
  181	UGACCUAUGAAUUGACAG	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0000272	AUGACCUAUGAAUUGACAGAC	573
  		215	215	215
  182	AAAUCUCUGCAGGCAAAUGUGA	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0004959	AAAUCUCUGCAGGCAAAUGUGA	574
  		216b	216b	216b
  183	AUACUGCAUCAGGAACUGAUUG	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0000274	UACUGCAUCAGGAACUGAUUGGA	575
  		217	217	217
  184	AGAGUUGAGUCUGGACGUCCCG	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0004567	AGAGUUGAGUCUGGACGUCCCG	576
  		219-1	219-1	219-1-3p
  185	AAGCUGCCAGUUGAAGAACUGU	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0000077	AAGCUGCCAGUUGAAGAACUGU	577
  		22	22	22
  186	AGCUACAUUGUCUGCUGGGUUUC	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0000278	AGCUACAUUGUCUGCUGGGUUUC	578
  		221	221	221
  187	AGCUACAUCUGGCUACUGGGU	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0000279	AGCUACAUCUGGCUACUGGGU	579
  		222	222	222
  188	CGUGUAUUUGACAAGCUGAGUU	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0004570	CGUGUAUUUGACAAGCUGAGUU	580
  		223*	223	223*
  189	CAAGUCACUAGUGGUUCCGUU	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0000281	CAAGUCACUAGUGGUUCCGUU	581
  		224	224	224
  190	AUCACAUUGCCAGGGAUUUCC	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0000078	AUCACAUUGCCAGGGAUUUCC	582
  		23a	23a	23a
  191	AUCACAUUGCCAGGGAUUACC	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0000418	AUCACAUUGCCAGGGAUUACC	583
  		23b	23b	23b
  192	UGGCUCAGUUCAGCAGGAACAG	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0000080	UGGCUCAGUUCAGCAGGAACAG	584
  		24-1	24-1	24
  193	UGGCUCAGUUCAGCAGGAACAG	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0000080	UGGCUCAGUUCAGCAGGAACAG	585
  		24-2	24-2	24
  194	CAUUGCACUUGUCUCGGUCUGA	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0000081	CAUUGCACUUGUCUCGGUCUGA	586
  		25	25	25
  195	UUCAAGUAAUCCAGGAUAGGCU	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0000082	UUCAAGUAAUCCAGGAUAGGCU	587
  		26a-1	26a-1	26a
  196	UUCAAGUAAUCCAGGAUAGGCU	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0000082	UUCAAGUAAUCCAGGAUAGGCU	588
  		26a-2	26a-2	26a
  197	UUCAAGUAAUUCAGGAUAGGU	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0000083	UUCAAGUAAUUCAGGAUAGGU	589
  		26b	26b	26b
  198	UUCACAGUGGCUAAGUUCCGC	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0000084	UUCACAGUGGCUAAGUUCCGC	590
  		27a	27a	27a
  199	UUCACAGUGGCUAAGUUCUGC	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0000419	UUCACAGUGGCUAAGUUCUGC	591
  		27b	27b	27b
  200	CACUAGAUUGUGAGCUCCUGGA	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0004502	CACUAGAUUGUGAGCUCCUGGA	592
  		28	28	28-3p
  201	GAGGGUUGGGUGGAGGCUCUCC	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0004679	GAGGGUUGGGUGGAGGCUCUCC	593
  		296-3p	296	296-3p
  202	AUGGUUUACCGUCCCACAUACA	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0002890	UGGUUUACCGUCCCACAUACAU	594
  		299-5p	299	299-5p
  203	UAGCACCAUCUGAAAUCGGUUA	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0000086	UAGCACCAUCUGAAAUCGGUUA	595
  		29a	29a	29a
  204	UAGCACCAUUUGAAAUCAGUGUU	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0000100	UAGCACCAUUUGAAAUCAGUGUU	596
  		29b-1	29b-1	29b
  205	UAGCACCAUUUGAAAUCAGUGUU	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0000100	UAGCACCAUUUGAAAUCAGUGUU	597
  		29b-2	29b-2	29b
  206	UAGCACCAUUUGAAAUCGGUUA	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0000681	UAGCACCAUUUGAAAUCGGUUA	598
  		29c	29c	29c
  207	CAGUGCAAUGAUAUUGUCAAAGC	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0004958	CAGUGCAAUGAUAUUGUCAAAGC	599
  		301b	301b	301b
  208	UGUAAACAUCCUCGACUGGAAG	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0000087	UGUAAACAUCCUCGACUGGAAG	600
  		30a	30a	30a
  209	UGUAAACAUCCUACACUCAGCU	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0000420	UGUAAACAUCCUACACUCAGCU	601
  		30b	30b	30b
  210	UGUAAACAUCCUACACUCUCAGC	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0000244	UGUAAACAUCCUACACUCUCAGC	602
  		30c-1	30c-1	30c
  211	UGUAAACAUCCUACACUCUCAGC	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0000244	UGUAAACAUCCUACACUCUCAGC	603
  		30c-2	30c-2	30c
  212	UGUAAACAUCCCCGACUGGAAG	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0000245	UGUAAACAUCCCCGACUGGAAG	604
  		30d	30d		30d
  213	UGUAAACAUCCUUGACUGGAAG	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0000692	UGUAAACAUCCUUGACUGGAAG	605
  		30e	30e	30e
  214	AGGCAAGAUGCUGGCAUAGCU	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0000089	AGGCAAGAUGCUGGCAUAGCU	606
  		31	31	31
  215	UAUUGCACAUUACUAAGUUGCA	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0000090	UAUUGCACAUUACUAAGUUGCA	607
  		32	32	32
  216	AAAAGCUGGGUUGAGAGGGCGA	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0000510	AAAAGCUGGGUUGAGAGGGCGA	608
  		320a	320a	320a
  217	AAAAGCUGGGUUGAGAGGGCAA	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0005792	AAAAGCUGGGUUGAGAGGGCAA	609
  		320b-1	320b-1	320b
  218	AAAAGCUGGGUUGAGAGGGCAA	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0005792	AAAAGCUGGGUUGAGAGGGCAA	610
  		320b-2	320b-2	320b
  219	AAAAGCUGGGUUGAGAGGGU	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0005793	AAAAGCUGGGUUGAGAGGGU	611
  		320c-1	320c-1	320c
  220	AAAAGCUGGGUUGAGAGGGU	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0005793	AAAAGCUGGGUUGAGAGGGU	612
  		320c-2	320c-2	320c
  221	AAAAGCUGGGUUGAGAGGA	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0006764	AAAAGCUGGGUUGAGAGGA	613
  		320d-1	320d-1	320d
  222	AAAAGCUGGGUUGAGAGGA	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0006764	AAAAGCUGGGUUGAGAGGA	614
  		320d-2	320d-2	320d
  223	CACAUUACACGGUCGACCUCU	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0000755	CACAUUACACGGUCGACCUCU	615
  		323	323	323-3p
  224	CGCAUCCCCUAGGGCAUUGGUGU	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0000761	CGCAUCCCCUAGGGCAUUGGUGU	616
  		324	324	5324-p
  225	CUGGCCCUCUCUGCCCUUCCGU	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0000752	CUGGCCCUCUCUGCCCUUCCGU	617
  		328	328	328
  226	GCAAAGCACACGGCCUGCAGAGA	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0000751	GCAAAGCACACGGCCUGCAGAGA	618
  		330	330	330-3p
  227	GCCCCUGGGCCUAUCCUAGAA	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0000760	GCCCCUGGGCCUAUCCUAGAA	619
  		331	331	331-3p
  228	UCAAGAGCAAUAACGAAAAAUGU	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0000765	UCAAGAGCAAUAACGAAAAAUGU	620
  		335	335	335
  229	UCCAGCAUCAGUGAUUUUGUUG	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0000763	UCCAGCAUCAGUGAUUUUGUUG	621
  		338	338	338-3p
  230	UGAGCGCCUCGACGACAGAGCCG	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0004702	UGAGCGCCUCGACGACAGAGCCG	622
  		339-3p	339	339-3p
  231	GUGCAUUGUAGUUGCAUUGCA	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0000091	GUGCAUUGUAGUUGCAUUGCA	623
  		33a	33a	33a
  232	GUGCAUUGCUGUUGCAUUGC	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0003301	GUGCAUUGCUGUUGCAUUGC	624
  		33b	33b	33b
  233	UUAUAAAGCAAUGAGACUGAUU	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0004692	UUAUAAAGCAAUGAGACUGAUU	625
  		340	340	340
  234	AGGGGUGCUAUCUGUGAUUGA	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0004694	AGGGGUGCUAUCUGUGAUUGA	626
  		342-5p	342	342-5p
  235	GCUGACUCCUAGUCCAGGGCUC	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0000772	GCUGACUCCUAGUCCAGGGCUC	627
  		345	345	345
  236	UGGCAGUGUCUUAGCUGGUUGU	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0000255	UGGCAGUGUCUUAGCUGGUUGU	628
  		34a	34a	34a
  237	AGGCAGUGUCAUUAGCUGAUUG	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0000685	UAGGCAGUGUCAUUAGCUGAUUG	629
  		34b*	34b	34b*
  238	AGGCAGUGUAGUUAGCUGAUUGC	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0000686	AGGCAGUGUAGUUAGCUGAUUGC	630
  		34c-5p	34c	34c-5p
  239	UUAUCAGAAUCUCCAGGGGUAC	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0000703	UUAUCAGAAUCUCCAGGGGUAC	631
  		361-5p	361	361-5p
  240	AACACACCUAUUCAAGGAUUCA	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0004683	AACACACCUAUUCAAGGAUUCA	632
  		362-3p	362	362-3p
  241	AAUUGCACGGUAUCCAUCUGUA	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0000707	AAUUGCACGGUAUCCAUCUGUA	633
  		363	363	363
  242	UAAUGCCCCUAAAAAUCCUUAU	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0000710	UAAUGCCCCUAAAAAUCCUUAU	634
  		365-2	365-2	365
  243	AAUAAUACAUGGUUGAUCUUU	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0000721	AAUAAUACAUGGUUGAUCUUU	635
  		369	369	369-3p
  244	GCCUGCUGGGGUGGAACCUGGU	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0000722	GCCUGCUGGGGUGGAACCUGGU	636
  		370	370	370
  245	ACUCAAACUGUGGGGGCACU	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0004687	ACUCAAACUGUGGGGGCACU	637
  		371	371	371-5p
  246	UUAUAAUACAACCUGAUAAGUG	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0000727	UUAUAAUACAACCUGAUAAGUG	638
  		374a	374a	374a
  247	AUAUAAUACAACCUGCUAAGUG	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0004955	AUAUAAUACAACCUGCUAAGUG	639
  		374b	374b	374b
  248	UUUGUUCGUUCGGCUCGCGUGA	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0000728	UUUGUUCGUUCGGCUCGCGUGA	640
  		375	375	375
  249	AGAGGUUGCCCUUGGUGAAUUC	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0004689	AGAGGUUGCCCUUGGUGAAUUC	641
  		377*	377	377*
  250	ACUGGACUUGGAGUCAGAAGG	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0000732	ACUGGACUUGGAGUCAGAAGG	642
  		378	378	378
  251	UGGUAGACUAUGGAACGUAGG	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0000733	UGGUAGACUAUGGAACGUAGG	643
  		379	379	379
  252	UAUACAAGGGCAAGCUCUCUGU	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0000736	UAUACAAGGGCAAGCUCUCUGU	644
  		381	381	381
  253	GAAGUUGUUCGUGGUGGAUUCG	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0000737	GAAGUUGUUCGUGGUGGAUUCG	645
  		382	382	382
  254	AGAUCAGAAGGUGAUUGUGGCU	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0000738	AGAUCAGAAGGUGAUUGUGGCU	646
  		383	383	383
  255	CGAAUGUUGCUCGGUGAACCCC	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0001639	GAAUGUUGCUCGGUGAACCCCU	647
  		409-3p	409	409-3p
  256	AAUAUAACACAGAUGGCCUGU	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0002171	AAUAUAACACAGAUGGCCUGU	648
  		410	410	410
  257	AUAGUAGACCGUAUAGCGUACG	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0003329	UAGUAGACCGUAUAGCGUACG	649
  		411	411	411
  258	AUCAACAGACAUUAAUUGGGCGC	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0003339	AUCAACAGACAUUAAUUGGGCGC	650
  		421	421	421
  259	UGAGGGGCAGAGAGCGAGACUUU	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0004748	UGAGGGGCAGAGAGCGAGACUUU	651
  		423	423	423-5p
  260	CAGCAGCAAUUCAUGUUUUGAA	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0001341	CAGCAGCAAUUCAUGUUUUGAA	652
  		424	424	424
  261	UAAUACUGUCUGGUAAAACCGU	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0001536	UAAUACUGUCUGGUAAAACCGU	653
  		429	429	429
  262	UCUUGGAGUAGGUCAUUGGGUGG	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0002814	UCUUGGAGUAGGUCAUUGGGUGG	654
  		432	432	432
  263	AUCAUGAUGGGCUCCUCGGUGU	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0001627	AUCAUGAUGGGCUCCUCGGUGU	655
  		433	433	433
  264	UUGCAUAUGUAGGAUGUCCCAU	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0001532	UUGCAUAUGUAGGAUGUCCCAU	656
  		448	448	448
  265	UGGCAGUGUAUUGUUAGCUGGU	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0001541	UGGCAGUGUAUUGUUAGCUGGU	657
  		449a	449a	449a
  266	AGGCAGUGUAUUGUUAGCUGGC	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0003327	AGGCAGUGUAUUGUUAGCUGGC	658
  		449b	449b	449b
  267	UUUUGCGAUGUGUUCCUAAUAU	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0001545	UUUUGCGAUGUGUUCCUAAUAU	659
  		450a-1	450a-1	450a
  268	UUUUGCGAUGUGUUCCUAAUAU	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0001545	UUUUGCGAUGUGUUCCUAAUAU	660
  		450a-2	450a-2	450a
  269	UUUUGCAAUAUGUUCCUGAAUA	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0004909	UUUUGCAAUAUGUUCCUGAAUA	661
  		450b-5p	450b	450b-5p
  270	AACUGUUUGCAGAGGAAACUGA	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0001635	AACUGUUUGCAGAGGAAACUGA	662
  		452	452	452
  271	UAGUGCAAUAUUGCUUAUAGGGU	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0003885	UAGUGCAAUAUUGCUUAUAGGGU	663
  		454	454	454
  272	GCAGUCCAUGGGCAUAUACAC	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0004784	GCAGUCCAUGGGCAUAUACAC	664
  		455-3p	455	455-3p
  273	AAGACGGGAGGAAAGAAGGGAG	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0004761	AAGACGGGAGGAAAGAAGGGAG	665
  		483-5p	483	483-5p
  274	UCAGGCUCAGUCCCCUCCCGAU	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0002174	UCAGGCUCAGUCCCCUCCCGAU	666
  		484	484	484
  275	AGAGGCUGGCCGUGAUGAAUUC	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0002175	AGAGGCUGGCCGUGAUGAAUUC	667
  		485-5p	485	485-5p
  276	UCCUGUACUGAGCUGCCCCGAG	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0002177	UCCUGUACUGAGCUGCCCCGAG	668
  		486-5p	486	486-5p
  277	AAUCGUACAGGGUCAUCCACUU	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0003180	AAUCGUACAGGGUCAUCCACUU	669
  		487b	487b	487b
  278	CCCAGAUAAUGGCACUCUCAA	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0002804	CCCAGAUAAUGGCACUCUCAA	670
  		488*	488	488*
  279	UUGUACAUGGUAGGCUUUCAUU	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0002813	UUGUACAUGGUAGGCUUUCAUU	671
  		493*	493	493*
  280	UGAAACAUACACGGGAAACCUC	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0002816	UGAAACAUACACGGGAAACCUC	672
  		494	494	494
  281	AAACAAACAUGGUGCACUUCUU	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0002817	AAACAAACAUGGUGCACUUCUU	673
  		495	495	495
  282	CAGCAGCACACUGUGGUUUGU	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0002820	CAGCAGCACACUGUGGUUUGU	674
  		497	497	497
  283	UUAAGACUUGCAGUGAUGUUU	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0002870	UUAAGACUUGCAGUGAUGUUU	675
  		499-5p	499	499-5p
  284	AUGCACCUGGGCAAGGAUUCUG	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0002871	AUGCACCUGGGCAAGGAUUCUG	676
  		500*	500	500*
  285	AAUGCACCCGGGCAAGGAUUCU	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0004774	AAUGCACCCGGGCAAGGAUUCU	677
  		501-3p	501	501-3p
  286	AAUGCACCUGGGCAAGGAUUCA	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0004775	AAUGCACCUGGGCAAGGAUUCA	678
  		502-3p	502	502-3p
  287	UAGCAGCGGGAACAGUUCUGCAG	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0002874	UAGCAGCGGGAACAGUUCUGCAG	679
  		503	503	503
  288	AGACCCUGGUCUGCACUCUAUC	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0002875	AGACCCUGGUCUGCACUCUAUC	680
  		504	504	504
  289	GGGAGCCAGGAAGUAUUGAUGU	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0004776	GGGAGCCAGGAAGUAUUGAUGU	681
  		505*	505	505*
  290	UGAUUGUAGCCUUUUGGAGUAGA	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0002880	UGAUUGUAGCCUUUUGGAGUAGA	682
  		508-3p	508	508-3p
  291	UACUGCAGACGUGGCAAUCAUG	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0004975	UACUGCAGACGUGGCAAUCAUG	683
  		509-3-5p	509-3	509-3-5p
  292	UUCACAGGGAGGUGUCAU	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0002877	UUCACAGGGAGGUGUCAU	684
  		513a-1	513a-1	513a-5p
  293	UUCACAGGGAGGUGUCAU	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0002877	UUCACAGGGAGGUGUCAU	685
  		513a-2	513a-2	513a-5p
  294	UUCACAAGGAGGUGUCAUUUAU	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0005788	UUCACAAGGAGGUGUCAUUUAU	686
  		513b	513b	513b
  295	UUCUCAAGGAGGUGUCGUUUAU	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0005789	UUCUCAAGGAGGUGUCGUUUAU	687
  		513c	513c	513c
  296	CAUGCCUUGAGUGUAGGACCGU	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0002888	CAUGCCUUGAGUGUAGGACCGU	688
  		532	532	532-5p
  297	UGUGACAGAUUGAUAACUGAAA	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0003389	UGUGACAGAUUGAUAACUGAAA	689
  		542-3p	542	542-3p
  298	AAACAUUCGCGGUGCACUUCUU	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0004954	AAACAUUCGCGGUGCACUUCUU	690
  		543	543	543
  299	CAAAACUGGCAAUUACUUUUGC	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0003251	CAAAACUGGCAAUUACUUUUGC	691
  		548a-1	548a-1	548a-3p
  300	CAAAACUGGCAAUUACUUUUGC	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0003251	CAAAACUGGCAAUUACUUUUGC	692
  		548a-2	548a-2	548a-3p
  301	CAAAACUGGCAAUUACUUUUGC	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0003251	CAAAACUGGCAAUUACUUUUGC	693
  		548a-3	548a-3	548a-3p
  302	CAAGAACCUCAGUUGCUUUUGU	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0003254	CAAGAACCUCAGUUGCUUUUGU	694
  		548b-3p	548b	548b-3p
  303	AAAAACUGAGACUACUUUUGCA	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0005874	AAAAACUGAGACUACUUUUGCA	695
  		548e	548e	548e
  304	AAAAGUAAUCGCGGUUUUUGUC	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0005928	AAAAGUAAUCGCGGUUUUUGUC	696
  		548h-1	548h-1	548h
  305	AAAAGUAAUCGCGGUUUUUGUC	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0005928	AAAAGUAAUCGCGGUUUUUGUC	697
  		548h-2	548h-2	548h
  306	AAAAGUAAUCGCGGUUUUUGUC	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0005928	AAAAGUAAUCGCGGUUUUUGUC	698
  		548h-3	548h-3	548h
  307	AAAAGUAAUCGCGGUUUUUGUC	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0005928	AAAAGUAAUCGCGGUUUUUGUC	699
  		548h-4	548h-4	548h
  308	AAAAGUAAUUGCGGUCUUUGGU	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0005875	AAAAGUAAUUGCGGUCUUUGGU	700
  		548j	548j	548j
  309	AAAAGUACUUGCGGAUUUUGCU	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0005882	AAAAGUACUUGCGGAUUUUGCU	701
  		548k	548k	548k
  310	AAAAGUAUUUGCGGGUUUUGUC	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0005889	AAAAGUAUUUGCGGGUUUUGUC	702
  		548l	548l	548l
  311	CAAAAGUAAUUGUGGAUUUUGU	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0005916	CAAAAGUAAUUGUGGAUUUUGU	703
  		548n	548n	548n
  312	AGUGCCUGAGGGAGUAAGAGCCC	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0004800	AGUGCCUGAGGGAGUAAGAGCCC	704
  		550-1	550-1	550
  313	AGUGCCUGAGGGAGUAAGAGCCC	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0004800	AGUGCCUGAGGGAGUAAGAGCCC	705
  		550-2	550-2	550
  314	GCGACCCAUACUUGGUUUCAG	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0003233	GCGACCCAUACUUGGUUUCAG	706
  		551b	551b	551b
  315	CACGCUCAUGCACACACCCACA	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0003239	CACGCUCAUGCACACACCCACA	707
  		574-3p	574	574-3p
  316	AAGAUGUGGAAAAAUUGGAAUC	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0004796	AAGAUGUGGAAAAAUUGGAAUC	708
  		576-3p	576	576-3p
  317	GUAGAUAAAAUAUUGGUACCUG	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0003242	UAGAUAAAAUAUUGGUACCUG	709
  		577	577	577
  318	UAACUGGUUGAACAACUGAACC	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0004797	UAACUGGUUGAACAACUGAACC	710
  		582-3p	582	582-3p
  319	UUACAGUUGUUCAACCAGUUACU	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0003247	UUACAGUUGUUCAACCAGUUACU	711
  		582-5p	582	582-5p
  320	UUAUGGUUUGCCUGGGACUGAG	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0003249	UUAUGGUUUGCCUGGGACUGAG	712
  		584	584	584
  321	UGAGAACCACGUCUGCUCUGAG	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0004799	UGAGAACCACGUCUGCUCUGAG	713
  		589	589	589
  322	UAAUUUUAUGUAUAAGCUAGU	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0004801	UAAUUUUAUGUAUAAGCUAGU	714
  		590-3p	590	590-3p
  323	UACGUCAUCGUUGUCAUCGUCA	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0003266	UACGUCAUCGUUGUCAUCGUCA	715
  		598	598	598
  324	UCCGAGCCUGGGUCUCCCUCUU	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0003283	UCCGAGCCUGGGUCUCCCUCUU	716
  		615-3p	615	615-3p
  325	AAGUCAUUGGAGGGUUUGAGCA	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0004805	AGUCAUUGGAGGGUUUGAGCAG	717
  		616	616	616
  326	AAACUCUACUUGUCCUUCUGAGU	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0003287	AAACUCUACUUGUCCUUCUGAGU	718
  		618	618	618
  327	AGGGGGAAAGUUCUAUAGUCC	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0003294	AGGGGGAAAGUUCUAUAGUCC	719
  		625	625	625
  328	AUGCUGACAUAUUUACUAGAGG	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0004809	AUGCUGACAUAUUUACUAGAGG	720
  		628-5p	628	628-5p
  329	UGGGUUUACGUUGGGAGAACU	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0004810	UGGGUUUACGUUGGGAGAACU	721
  		629	629	629
  330	AAAGACAUAGGAUAGAGUCACCUC	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0003311	AAAGACAUAGGAUAGAGUCACCUC	722
  		641	641	641
  331	ACACUUGUAUGCUAGCUCAGGU	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0003313	ACUUGUAUGCUAGCUCAGGUAG	723
  		643	643	643
  332	UUUAGGAUAAGCUUGACUUUUG	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0003321	UUUAGGAUAAGCUUGACUUUUG	724
  		651	651	651
  333	AAUGGCGCCACUAGGGUUGUG	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0003322	AAUGGCGCCACUAGGGUUGUG	725
  		652	652	652
  334	UAUGUCUGCUGACCAUCACCUU	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0004814	UAUGUCUGCUGACCAUCACCUU	726
  		654-3p	654	654-3p
  335	UACCCAUUGCAUAUCGGAGUUG	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0003338	UACCCAUUGCAUAUCGGAGUUG	727
  		660	660	660
  336	UCCGGUUCUCAGGGCUCCACC	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0004819	UCCGGUUCUCAGGGCUCCACC	728
  		671-3p	671	671-3p
  337	AAGGAGCUUACAAUCUAGCUGGG	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0004926	AAGGAGCUUACAAUCUAGCUGGG	729
  		708	708	708
  338	UGGAAGACUAGUGAUUUUGUUGU	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0000252	UGGAAGACUAGUGAUUUUGUUGU	730
  		7-1	7-1	7
  339	UGGAAGACUAGUGAUUUUGUUGU	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0000252	UGGAAGACUAGUGAUUUUGUUGU	731
  		7-2	7-2	7
  340	UGGAAGACUAGUGAUUUUGUUGU	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0000252	UGGAAGACUAGUGAUUUUGUUGU	732
  		7-3	7-3	7
  341	CGGCUCUGGGUCUGUGGGGA	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0004957	CGGCUCUGGGUCUGUGGGGA	733
  		760	760	760
  342	UGCACCAUGGUUGUCUGAGCAUG	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0003882	UGCACCAUGGUUGUCUGAGCAUG	734
  		767-5p	767	767-5p
  343	UGAGACCUCUGGGUUCUGAGCU	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0003886	UGAGACCUCUGGGUUCUGAGCU	735
  		769-5p	769	769-5p
  344	GCAGGAACUUGUGAGUCUCCU	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0004953	GCAGGAACUUGUGAGUCUCCU	736
  		873	873	873
  345	CUGCCCUGGCCCGAGGGACCGA	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0004911	CUGCCCUGGCCCGAGGGACCGA	737
  		874	874	874
  346	GUAGAGGAGAUGGCGCAGGG	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0004949	GUAGAGGAGAUGGCGCAGGG	738
  		877	877	877
  347	GUGAACGGGCGCCAUCCCGAGG	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0004951	GUGAACGGGCGCCAUCCCGAGG	739
  		887	887	887
  348	UUAAUAUCGGACAACCAUUGU	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0004921	UUAAUAUCGGACAACCAUUGU	740
  		889	889	889
  349	UGCAACGAACCUGAGCCACUGA	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0004902	UGCAACGAACCUGAGCCACUGA	741
  		891a	891a	891a
  350	CACUGUGUCCUUUCUGCGUAG	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0004907	CACUGUGUCCUUUCUGCGUAG	742
  		892a	892a	892a
  351	UCUUUGGUUAUCUAGCUGUAUGA	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0000441	UCUUUGGUUAUCUAGCUGUAUGA	743
  		9-1	9-1	9
  352	UCUUUGGUUAUCUAGCUGUAUGA	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0000441	UCUUUGGUUAUCUAGCUGUAUGA	744
  		9-2	9-2	9
  353	UAUUGCACUUGUCCCGGCCUGU	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0000092	UAUUGCACUUGUCCCGGCCUGU	745
  		92a-1	92a-1	92a
  354	UAUUGCACUUGUCCCGGCCUGU	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0000092	UAUUGCACUUGUCCCGGCCUGU	746
  		92a-2	92a-2	92a
  355	UAUUGCACUCGUCCCGGCCUCC	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0003218	UAUUGCACUCGUCCCGGCCUCC	747
  		92b	92b	92b
  356	CAAAGUGCUGUUCGUGCAGGUAG	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0000093	CAAAGUGCUGUUCGUGCAGGUAG	748
  		93	93	93
  357	UCUUUGGUUAUCUAGCUGUAUGA	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0000441	UCUUUGGUUAUCUAGCUGUAUGA	749
  		9-3	9-3	9
  358	AAGGCAGGGCCCCCGCUCCCC	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0004983	AAGGCAGGGCCCCCGCUCCCC	750
  		940	940	940
  359	UUCUCUGUUUUGGCCAUGUGUG	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0004985	UCUUCUCUGUUUUGGCCAUGUG	751
  		942	942	942
  360	AAAUUAUUGUACAUCGGAUGAG	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0004987	AAAUUAUUGUACAUCGGAUGAG	752
  		944	944	944
  361	UUCAACGGGUAUUUAUUGAGCA	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0000094	UUCAACGGGUAUUUAUUGAGCA	753
  		95	95	95
  362	UUUGGCACUAGCACAUUUUUGCU	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0000095	UUUGGCACUAGCACAUUUUUGCU	754
  		96	96	96
  363	UGAGGUAGUAAGUUGUAUUGUU	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0000096	UGAGGUAGUAAGUUGUAUUGUU	755
  		98	98	98
  364	AACCCGUAGAUCCGAUCUUGUG	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0000097	AACCCGUAGAUCCGAUCUUGUG	756
  		99a	99a	99a
  365	CACCCGUAGAACCGACCUUGCG	hsa-mir-	hsa-mir-	hsa-miR-	MIMAT0000689	CACCCGUAGAACCGACCUUGCG	757
  		99b	99b	99b
  366	AACAUAGAGGAAAUUCCACGU	hsa-mir-	hsa-mir-	hsa-mir-		AACAUAGAGGAAAUUCCACGU	758
  		376c	376c	376c
  367	AUCAUAGAGGAAAAUCCAUGUU	hsa-mir-	hsa-mir-	hsa-mir-		AUCAUAGAGGAAAAUCCAUGUU	759
  		376b	376b	376b
  368	UGGUGGGCCGCAGAACAUGUGC	hsa-mir-	hsa-mir-	hsa-mir-		UGGUGGGCCGCAGAACAUGUGC	760
  		654	654	654
  369	AUCAUAGAGGAAAAUCCACGU	hsa-mir-	hsa-mir-	hsa-mir-		AUCAUAGAGGAAAAUCCACGU	761
  		376a-2	376a-2	376a-2
  370	AUCAUAGAGGAAAAUCCACGU	hsa-mir-	hsa-mir-	hsa-mir-		AUCAUAGAGGAAAAUCCACGU	762
  		376a-1	376a-1	376a-1
  371	AAAACCGUCUAGUUACAGUUGU	hsa-mir-
  		1537
  372	AUAUACAGGGGGAGACUCUCAU	hsa-mir-
  		1185-2
  373	CCUAGAAUGGGGAUUGUGGG	hsa-mir-
  		1301*
  374	AGCGAGACCUCAACUCUACAAU	hsa-mir-
  		1303
  375	UCGACCGGACCUCGACCGGCUC	hsa-mir-
  		1307*
  376	AUGUAGGGAUGGAAGCCAUGAA	hsa-mir-
  		135a-2
  377	CUCACUGAACAAUGAAUGCAA	hsa-mir-
  		181b-1*
  378	CGGGUAGAGAGGGCAGUGGGAG	hsa-mir-
  		197*
  379	GCUGGGAAGGCAAAGGGACGU	hsa-mir-
  		204*
  380	ACCUUGGCUCUAGACUGCUUAC	hsa-mir-
  		212*
  381	GCUCUGACUUUAUUGCACUACU	hsa-mir-
  		301a
  382	AGGGACUUUUGGGGGCAGAUGU	hsa-mir-
  		365-1
  383	UUGGGGACAUUUUGCAUUCAU	hsa-mir-
  		450a-2*
  384	AAUGUGUAGCAAAAGACAGA	hsa-mir-
  		511-1-3p
  385	AAUGUGUAGCAAAAGACAGA	hsa-mir-
  		511-2-3p
  386	AUCAUACAAGGACAAUUUCUUU	hsa-mir-
  		539
  387	AAAGGUAAUUGCAGUUUUUCCC	hsa-mir-
  		570
  388	UCGCGGUUUGUGCCAGAUGACG	hsa-mir-
  		579
  389	AGAAGGCACUAUGAGAUUUAGA	hsa-mir-
  		605
  390	AGACACAUUUGGAGAGGGAACC	hsa-mir-
  		642
  391	AGGACCUUCCCUGAACCAAGGA	hsa-mir-
  		659
  392	AUAUACAGGGGGAGACUCUUAU	hsa-mir-
  		1185-1

• TABLE 32
  Novel miRNAs identified by deep sequencing analysis.

  SEQ		Temporary		SEQ
  ID	Mature	Assigned	miRBase	ID
  NO.	Sequence Captured	miRNA ID	ID	NO.	miRNA Precursor Sequence

  763	CCAAAACUGCAGUUACUUUUG	has-mir-548o-	pending	1057	UggUgcaaaagUaaUUgcggUUUUUgccaUUaaaagUaaUgc
  		2			ggCCAAAACUGCAGUUACUUUUGcaccc
  764	GAGCCUGGAAGCUGGAGCCUGC	hsa-mir-1254-	hsa-mir-	1058	cUGAGCCUGGAAGCUGGAGCCUGCagUgagcUaUgaUca
  		2	1254-2		UgUcccUgUacUcUagccUgggca
  765	CGGGCGUGGUGGUGGGGGUG	hsa-mir-1268b	hsa-mir-	1059	accCGGGCGUGGUGGUGGGGGUGggUgccUgUaaUUcca
  			1268b		gcUagUUggga
  766	UCGAGGAGCUCACAGUCUAGA	hsa-miR-151-	hsa-mir-	1060	agUcUcUcUUcagggcUcccgagacacagaaacagacaccUgccc
  		5p-2	151b		UCGAGGAGCUCACAGUCUAGAca
  767	UAAGGUGCAUCUAGUGCAGUU	hsa-mir-18b-	hsa-mir-	1061	UcUUgUgUUAAGGUGCAUCUAGUGCAGUUagUgaagcag
  		2	18b		cUUagaaUcUacUgcccUaaaUgccccUUcUggcacaggc
  768	GAGGGUUGGGUGGAGGC	hsa-miR-296-	hsa-miR-	1062	cUgccUccaccccgccUggccUgacccagccagggcUcUagGAGG
  		2	296-2		GUUGGGUGGAGGCaa
  769	AUCAUAGAGGAAAAUCCAUGUU	hsa-mir-376b-	hsa-mir-	1063	UaaaacgUggaUaUUccUUcUaUgUUUacgUgaUUccUggU
  		2	376b		UaAUCAUAGAGGAAAAUCCAUGUUUUc
  770	AACAUAGAGGAAAUUCCACGU	hsa-mir-376c-	hsa-mir-	1064	UaaaaggUggaUaUUccUUcUaUgUUUaUgUUaUUUaUgg
  		2	376c		UUaAACAUAGAGGAAAUUCCACGUUUUc
  771	ACUGGACUUGGAGUCAGAAA	hsa-mir-378b-	hsa-mir-	1065	acUgUUUcUgUccUUgUUcUUgUUgUUaUUACUGGACU
  		1	378d-1		UGGAGUCAGAAAcagg
  772	ACUGGACUUGGAGUCAGAAA	hsa-mir-378b-	hsa-mir-	1066	aggagagaacACUGGACUUGGAGUCAGAAAacUUUcaUcc
  		2	378d-2		aagUcaUUcccUgcUcUaagUcccaUUUcUgUUcca
  773	ACUGGACUUGGAGUCAGGA	hsa-miR-378c	hsa-mir-	1067	cUgacUccagUgUccaggccaggggcagacagUggacagagaacag
  			378e		UgcccaagaccACUGGACUUGGAGUCAGGAcaU
  774	AGGCAGUGUAUUGCUAGCGGCU	hsa-mir-449c	hsa-mir-	1068	UcaggUAGGCAGUGUAUUGCUAGCGGCUgUUaaUgaUU
  			449c		UUaacagUUgcUagUUgcacUccUcUcUgU
  775	UGCACCCAGGCAAGGAUUCUGC	hsa-mir-500-	hsa-mir-	1069	gUUcccccUcUcUaaUccUUgcUaccUgggUgagagUgcUUUc
  		2	500b		UgaaUgcagUGCACCCAGGCAAGGAUUCUGCaagggggag
  					U
  776	UAAUCCUUGCUACCUGGGUGAG	hsa-mir-500-	has-mir-	1070	gUUcccccUcUcUAAUCCUUGCUACCUGGGUGAGagUgc
  		2*	500b		UUUcUgaaUgcagUgcacccaggcaaggaUUcUgcaagggggag
  					U
  777	AAAAGUAAUCGCGGUUUUUGUC	hsa-mir-548h-	hsa-mir-	1071	acAAAAGUAAUCGCGGUUUUUGUCaUUacUUUUaacUg
  		5	548h-5		UaaaaaccacggUUgcUUUUgc
  778	AAAAGUAAUUGUGGAUUUUGCU	hsa-mir-548r-	hsa-mir-	1072	aUgUUggUgcAAAAGUAAUUGUGGAUUUUGCUaUUacU
  		1	548ab		UgUaUUUaUUUgUaaUgcaaaacccgcaaUUagUUUUgcacc
  					aacc
  779	AAAAGUAAUUGUGGAUUUUGCU	hsa-mir-548r-	has-mir-	1073	UgUUggUgcAAAAGUAAUUGUGGAUUUUGCUaUUacU
  		2	548ab		UgUaUUUaUUUgUaaUgcaaaacccgcaaUUagUUUUgcacc
  					aacc
  780	UGUCUUACUCCCUCAGGCACAU	hsa-miR-550-	pending	1074	cUggUgcagUgccUgagggagUaagagUccUgUUgUUgUaaga
  		3*			UagUGUCUUACUCCCUCAGGCACAUcUccaa
  781	GGUGGGCUUCCCGGAGGG	hsa-mir-2221	hsa-mir-	1075	gaaaacaaccaGGUGGGCUUCCCGGAGGGcggaacacccagc
  			4417		cccagcaUccagggcUcaccUaccacgUUUg
  782	GAAGCGGUGGCUGGGCUG	hsa-mir-2222	pending	1076	gaggcggcccUagcgccaUUUUgUgggagcGAAGCGGUGGCU
  					GGGCUGcgcUUg
  783	CACUGCAGGACUCAGCAG	hsa-mir-2223	hsa-mir-	1077	UggUUUUUgcUcUgagUgaccgUggUggUUgUgggagUCAC
  			4418		UGCAGGACUCAGCAGgaaUUc
  784	UGAGGGAGGAGACUGCA	hsa-mir-2224	hsa-mir-	1078	UggUggUgUgUgccUgUagUcUUagcUacUcgggaggcUGAG
  			4419a		GGAGGAGACUGCAgUgagUggaggUcacgccacUg
  785	ACUGGACUUGGAGCCAGAAG	hsa-mir-2225	hsa-mir-	1079	gUcaggUccUggacUcccaUagUUUUcaggcUgcUaaacaacag
  			378f		aacgagcACUGGACUUGGAGCCAGAAGUcUUggg
  786	GUCACUGAUGUCUGUAGCUGAG	hsa-mir-2226	hsa-mir-	1080	cUcUUggUaUgaacaUcUgUgUgUUcaUgUcUcUcUgUgca
  			4420		caggggacgagaGUCACUGAUGUCUGUAGCUGAGac
  787	GAUGAGGAUGGAUAGCAAGGAA	hsa-mir-2227	hsa-mir-	1081	cUggccUcUgUgccUggaUacUUUaUacgUgUaaUUgUGAU
  			3605		GAGGAUGGAUAGCAAGGAAgccgc
  788	ACCUGUCUGUGGAAAGGAGCUA	hsa-mir-2228	hsa-mir-	1082	cUgggUcUccUUUcUgcUgagagUUgaacacUUgUUgggaca
  			4421		ACCUGUCUGUGGAAAGGAGCUAccUac
  789	AAAAGCAUCAGGAAGUACCCA	hsa-mir-2229	hsa-mir-	1083	agUUcUUcUgcagacAAAAGCAUCAGGAAGUACCCAccaU
  			4422		gUaccagUgggcccUUcUUgaUgcUcUUgaUUgcagaggagcc
  790	UGCCUGGAACAUAGUAGGGACU	hsa-mir-2230-	hsa-mir-	1084	UccUUUaUUgagUcccUacUaUgUUccaggcaccUacgaUacc
  		1	3116-2		cagUGCCUGGAACAUAGUAGGGACUcaaUaaagU
  791	UGCCUGGAACAUAGUAGGGACU	hsa-mir-2230-	hsa-mir-	1085	ccUUUaUUgagUcccUacUaUgUUccaggcaccUacgaUaccc
  		2	3116-1		agUGCCUGGAACAUAGUAGGGACUcaaUaaagU
  792	UGGAUUAAAAACAAUGGAGG	hsa-mir-2231	pending	1086	cgccUccaUgUUUcagcaUcUaUgUcaUgggcUUggUccUgga
  					gUGGAUUAAAAACAAUGGAGGU
  793	AUAGGCACCAAAAAGCAACAA	hsa-mir-2232	hsa-mir-	1087	aUcaUgUacUgcagUUgccUUUUUgUUcccaUgcUgUUUaa
  			4423		gccUagcAUAGGCACCAAAAAGCAACAAcagUaUgUgaa
  794	ACUGGGCUUGGAGUCAGAAG	hsa-mir-2233	hsa-mir-	1088	cACUGGGCUUGGAGUCAGAAGaccUggcUccagcccagcUc
  			378g		gUaUUaggUUggUgcaaaagUUaUUgUggUUUUUgcUaUU
  795	CAAAAACCGGCAAUUACUUUUG	hsa-mir-2234	hsa-mir-	1089	UUUUUUUaaUggCAAAAACCGGCAAUUACUUUUGcacU
  			548ac		aaccUagUag
  796	GUCAAAUGAAGGGCUGAUCACG	hsa-mir-2235	pending	1090	aaagUGUCAAAUGAAGGGCUGAUCACGaaaUagcgcaUU
  					agcUcUUUUUUUgaaaacUUg
  797	AAAGGGAGGAUUUGCUUAGAAGG	hsa-mir-2236	pending	1091	gacUggcUacgUagUUcgggcaaaUccUccaaaagggAAAGGG
  	AUGG				AGGAUUUGCUUAGAAGGAUGGcgcUcc
  798	AGAGUUAACUCAAAAUGGACUA	hsa-mir-2237	hsa-mir-	1092	cUUacaUcacacacAGAGUUAACUCAAAAUGGACUAaUU
  			4424		UUUccacUagUUagUccaUUUcaagUUaacUcUgUgUgUga
  					UgUagU
  799	UGUUGGGAUUCAGCAGGACCAU	hsa-mir-2238	hsa-mir-	1093	gUgcUUUacaUgaaUggUcccaUUgaaUcccaacagcUUUgcg
  			4425		aagUgUUGUUGGGAUUCAGCAGGACCAUUcgUgUaaag
  					Uaa
  800	UAAAUAGAGUAGGCAAAGGACA	hsa-mir-2239	hsa-mir-	1094	UaaaUggUUaUgUccUUUgccUaUUcUaUUUaagacacccU
  			3121		gUaccUUAAAUAGAGUAGGCAAAGGACAgaaacaUUUU
  801	AGAGUCGAGAGUGGGAGAAGAG	hsa-mir-2240	pending	1095	gcAGAGUCGAGAGUGGGAGAAGAGcggagcgUgUgagcag
  					UacUgcggccUccUcUccUcUccUaaccUcgcUcUc
  802	GAAGAUGGACGUACUUU	hsa-mir-2241	hsa-mir-	1096	agUUGAAGAUGGACGUACUUUgUcUgacUacaaUaUUca
  			4426		aaaggagUcUacUcUUcaUcUUg
  803	UCUGAAUAGAGUCUGAAGAGU	hsa-mir-2242	hsa-mir-	1097	gaagccUcUUggggcUUaUUUagacaaUggUUUcaUcaUUUc
  			4427		gUCUGAAUAGAGUCUGAAGAGUcUUU
  804	CAAGGAGACGGGAACAUGGAGC	hsa-mir-2243	hsa-mir-	1098	UUggcaggUgccaUgUUgccUgcUccUUacUgUacacgUggcU
  			4428		ggCAAGGAGACGGGAACAUGGAGCcgccaU
  805	AAGAGGAAGAAAUGGCUGGUUC	hsa-mir-2244	hsa-mir-	1099	aggaagAAGAGGAAGAAAUGGCUGGUUCUcaggUgaaUg
  			3916		UgUcUgggUUcaggggaUgUgUcUccUcUUUUcU
  806	UUCGCGGGCGAAGGCAAAGUC	hsa-mir-2245	hsa-mir-	1100	ggcgggcUUCGCGGGCGAAGGCAAAGUCgaUUUccaaaag
  			3124		UgacUUUccUcacUcccgUgaagUcggcg
  807	AAAAGCUGGGCUGAGAGGCG	hsa-mir-2246	hsa-mir-	1101	agggagAAAAGCUGGGCUGAGAGGCGacUggUgUcUaaU
  			4429		UUgUUUgUcUcUccaacUcagacUgccUggccca
  808	AUGGCCAAAACUGCAGUUAUUU	hsa-mir-2247	hsa-mir-	1102	cUgcaaaaaUaaUUgcagUUUUUgccaUUaUUUUUaaUaa
  			548s		UUaUaaUaAUGGCCAAAACUGCAGUUAUUUUUgcac
  809	UAGUGGAUGAUGCACUCUGUGC	hsa-mir-2248	hsa-mir-	1103	cUacUUccagUAGUGGAUGAUGCACUCUGUGCagggccaa
  			3681		cUgUgcacacagUgcUUcaUccacUacUggaagUgU
  810	AGGCUGGAGUGAGCGGAG	hsa-mir-2249	hsa-mir-	1104	gUgAGGCUGGAGUGAGCGGAGaUcgUaccacUgcacUcca
  			4430		accUggUga
  811	GAAAACGACAAUGACUUUUGCA	hsa-mir-2250	hsa-mir-	1105	cUgUUaggUUggUgcaaaagUaaUUgUggUUUUUgaaagUa
  			548ad		acUUggcGAAAACGACAAUGACUUUUGCAccaaUcUaaUa
  					c
  812	GCGACUCUGAAAACUAGAAGGU	hsa-mir-2251	hsa-mir-	1106	UggUUUGCGACUCUGAAAACUAGAAGGUUUaUgacUgg
  			4431		gcaUUUcUcacccaaUgcccaaUaUUgaacUUUcUagUUgUc
  					agagUcaUUaaccc
  813	AGAAGGCUGGAGCGCGGCGGU	hsa-mir-2252	pending	1107	gcacUgcggUUcUgaggccgUUacUccggcUUcUccaUagaggg
  					cggAGAAGGCUGGAGCGCGGCGGUga
  814	AAAGACUCUGCAAGAUGCCU	hsa-mir-2253	hsa-mir-	1108	gcaUcUUgcagagccgUUccaaUgcgacaccUcUagagUgUcaU
  			4432		ccccUagaaUgUcaccUUggAAAGACUCUGCAAGAUGCCU
  815	ACAGGAGUGGGGGUGGGACAU	hsa-mir-2254	hsa-mir-	1109	caUccUccUUacgUcccaccccccacUccUgUUUcUggUgaaaU
  			4433		aUUcaaACAGGAGUGGGGGUGGGACAUaaggaggaUa
  816	AGGAGAAGUAAAGUAGAA	hsa-mir-2255	hsa-mir-	1110	UcacUUUAGGAGAAGUAAAGUAGAAcUUUggUUUUcaa
  			4434		cUUUUccUacagUgU
  817	AUGGCCAGAGCUCACACAGAGG	hsa-mir-2256	hsa-mir-	1111	aggcagcaaAUGGCCAGAGCUCACACAGAGGgaUgagUgca
  			4435-1		cUUcaccUgcagUgUgacUcagcaggccaacagaUgcUa
  818	GCAGGACAGGCAGAAGUGGAU	hsa-mir-2257	hsa-mir-	1112	gccUcacUUUUccacUUaUgccUgcccUgccccUcgaaUcUgcU
  			4436		ccacgaUUUggGCAGGACAGGCAGAAGUGGAUaagUgagg
  					a
  819	AUCAGGGCUUGUGGAAUGGGAA	hsa-mir-2258	hsa-mir-	1113	ggcccAUCAGGGCUUGUGGAAUGGGAAggagaagggacgc
  			3127		UUccccUUcUgcaggccUgcUgggUg
  820	AUGGCCAGAGCUCACACAGAGG	hsa-mir-2259	hsa-mir-	1114	gcaaAUGGCCAGAGCUCACACAGAGGgaUgagUgcacUUc
  			4435-2		accUgcagUgUgacUcagcaggccaacagaUgcU
  821	UGAGGAUAUGGCAGGGAAGGGG	hsa-mir-2260	hsa-mir-	1115	acgUggUGAGGAUAUGGCAGGGAAGGGGagUUUcccUc
  			3679		UaUUcccUUccccccagUaaUcUUcaUcaUgc
  822	AGGAGGUUGGUGUGGAUU	hsa-mir-2261	pending	1116	UaUgAGGAGGUUGGUGUGGAUUcUgUUgaagaaaaagaa
  					ggggaacacUaaUUUUccaUU
  823	UUGGAAGACAUGGAGCAUGAGG	hsa-mir-2262	pending	1117	UUUUGGAAGACAUGGAGCAUGAGGUaagUgccUagaUcc
  					UcaaaccacUUgccUccaccUaUgcUUccaggU
  824	UCUGGCAAGUAAAAAACUCUCA	hsa-mir-2263	hsa-mir-	1118	cUUccUCUGGCAAGUAAAAAACUCUCAUUUUccUUaaaa
  			3128		aaUgagagUUUUUUacUUgcaaUaggaaa
  825	UGGGCUCAGGGUACAAAGGUU	hsa-mir-2264	hsa-mir-	1119	acUUUgUgcaUUgggUccacaaggaggggaUgacccUUgUGG
  			4437		GCUCAGGGUACAAAGGUU
  826	CAAAAACUGCAAUUACUUUCA	hsa-mir-2265-	hsa-mir-	1120	gcagUUUUUgccaUUaagUUgcggUUUUUgccaUUaUaaUg
  		1	548ae-1		gCAAAAACUGCAAUUACUUUCAcaccUgc
  827	CAAAAACUGCAAUUACUUUCA	hsa-mir-2265-	hsa-mir-	1121	UgUgcaaaagUaaUUgUggUUUUUgUcaUUUaaaagUaaUg
  		2	548ae-2		gCAAAAACUGCAAUUACUUUCAcacc
  828	GCUGCACCGGAGACUGGGUAA	hsa-mir-2266-	hsa-mir-	1122	acUUgUcaUgUcUUacccagUcUccggUgcagccUgUUgUcaa
  		1	3130-1		gGCUGCACCGGAGACUGGGUAAgacaUgacaagc
  829	GCUGCACCGGAGACUGGGUAA	hsa-mir-2266-	hsa-mir-	1123	UgUcUUacccagUcUccggUgcagccUUgacaacagGCUGCAC
  		2	3130-2		CGGAGACUGGGUAAgacaUgacaagUU
  830	AUCCCCAGAUACAAUGGACAAU	hsa-mir-2267	hsa-mir-	1124	cgUgUcAUCCCCAGAUACAAUGGACAAUaUgcUaUUaUa
  			2355		aUcgUaUggcaUUgUccUUgcUgUUUggagaUaaUacU
  831	AUUGUCCUUGCUGUUUGGAGAU	hsa-mir-2267*	pending	1125	cgUgUcaUccccagaUacaaUggacaaUaUgcUaUUaUaaUcg
  					UaUggcAUUGUCCUUGCUGUUUGGAGAUaaUacU
  832	CACAGGCUUAGAAAAGACAGU	hsa-mir-2268	hsa-mir-	1126	UaagUgUaaacUUaaggacUgUcUUUUcUaagccUgUgccUU
  			4438		gccUUUccUUUggCACAGGCUUAGAAAAGACAGUcUUUa
  					agUUUacacUUc
  833	UCGAGGACUGGUGGAAGGGCCU	hsa-mir-2269	hsa-mir-	1127	UcUcagagUCGAGGACUGGUGGAAGGGCCUUUccccUca
  			3131		gaccaaggcccUggccccagcUUcUUcUcagagU
  834	GUGACUGAUACCUUGGAGGCAU	hsa-mir-2270	hsa-mir-	1128	ccaGUGACUGAUACCUUGGAGGCAUUUUaUcUaagaUac
  			4439		acacaaagcaaaUgccUcUaaggUaUcagUUUaccaggcca
  835	AGGGCUGGACUCAGCGGCGGAG	hsa-mir-2271	pending	1129	gcgcagAGGGCUGGACUCAGCGGCGGAGcUggcUgcUggc
  					cUcagUUcUgccUcUgUccaggUccUUgUga
  836	UGUCGUGGGGCUUGCUGGCUUG	hsa-mir-2272	hsa-mir-	1130	cUcUcaccaagcaagUgcagUggggcUUgcUggcUUgcaccgUg
  			4440		acUcccUcUcaccaagcaagUGUCGUGGGGCUUGCUGGCU
  					UGcacUgUgaagaU
  837	ACAGGGAGGAGAUUGUA	hsa-mir-2273	hsa-mir-	1131	cagagUcUccUUcgUgUacagggaggagacUgUacgUgagagaU
  			4441		agUcagaUccgcaUgUUagagcagagUcUccUUcgUgUACAG
  					GGAGGAGAUUGUAc
  838	ACUGGACUUGGAGGCAGAA	hsa-mir-2274	hsa-mir-	1132	UggUcaUUgagUcUUcaaggcUagUggaaagagcACUGGACU
  			378b		UGGAGGCAGAAagaccc
  839	GCCGGACAAGAGGGAGG	hsa-mir-2275	hsa-mir-	1133	gcgcccUcccUcUcUccccggUgUgcaaaUgUgUgUgUgcggUg
  			4442		UUaUGCCGGACAAGAGGGAGGUg
  840	AUACACAUACACGCAACACACA	hsa-mir-2276	hsa-mir-	1134	aUgUgUgUgUaUaUgUgUgUUgcaUgUgUgUaUaUgUgUg
  			466		UaUaUaUgUacacAUACACAUACACGCAACACACAUaUa
  					UacaUgcac
  841	UUGGAGGCGUGGGUUUU	hsa-mir-2277	hsa-mir-	1135	ggUgggggUUGGAGGCGUGGGUUUUagaaccUaUcccUU
  			4443		UcUagcccUgagca
  842	AAUUCCCUUGUAGAUAACCCGG	hsa-mir-2278	hsa-mir-	1136	cgaUcacUagaUUaUcUacaagggaaUUUUUUUUUaaUUU
  			3938		aaaaAAUUCCCUUGUAGAUAACCCGGUggUca
  843	AAGACUGGAGACAAAGUGGGAG	hsa-mir-2279	pending	1137	CACCACCAAAAUCUCCAGGGGCAUCGUUGAAAUCGUA
  					AGGGAUGUGCAGCUCAUUAAGACUGGAGACAAAGUG
  					GGAG
  844	CUGACUGAAUAGGUAGGGUCAU	hsa-mir-2280	hsa-mir-	1138	aaaCUGACUGAAUAGGUAGGGUCAUUUUUcUgUgacUg
  			3136		cacaUggcccaaccUaUUcagUUagUUc
  845	CUCGAGUUGGAAGAGGCG	hsa-mir-2281	hsa-mir-	1139	gUgacgacUggccccgccUcUUccUcUcggUcccaUaUUgaaCU
  			4444		CGAGUUGGAAGAGGCGagUccggUcUcaaa
  846	AGAUUGUUUCUUUUGCCGUGCA	hsa-mir-2282	hsa-mir-	1140	UUccUgcAGAUUGUUUCUUUUGCCGUGCAagUUUaagU
  			4445		UUUUgcacggcaaaagaaacaaUccagagggU
  847	CACGGCAAAAGAAACAAUCCA	hsa-mir-2282*	has-mir-	1141	UUccUgcagaUUgUUUcUUUUgccgUgcaagUUUaagUUU
  			4445		UUgCACGGCAAAAGAAACAAUCCAgagggU
  848	CAGGGCUGGCAGUGACAUGGGU	hsa-mir-2283	hsa-mir-	1142	cUggUccaUUUcccUgccaUUcccUUggcUUcaaUUUacUcc
  			4446		CAGGGCUGGCAGUGACAUGGGUcaa
  849	GGUGGGGGCUGUUGUUU	hsa-mir-2284	hsa-mir-	1143	gUUcUagagcaUggUUUcUcaUcaUUUgcacUacUgaUacU
  			4447		UggggUcagaUaaUUgUUUgUGGUGGGGGCUGUUGUUU
  					gcaUUgUaggaU
  850	UGGGGAGGUGUGGAGUCAGCAU	hsa-mir-2285	pending	1144	gggcaUGGGGAGGUGUGGAGUCAGCAUggggcUaggaggc
  					cccgcgcUgacccgccUUcUccgcagcUg
  					accUgagcaccaUUUacUgagUccUUUgUUcUcUacUagUUU
  851	GCAGAGAACAAAGGACUCAGU	hsa-mir-2286	hsa-mir-	1145	gUagUagUUcgUaGCAGAGAACAAAGGACUCAGUaaaUg
  			3919		gUgcUcagga
  852	GGCUCCUUGGUCUAGGGGUA	hsa-mir-2287	hsa-mir-	1146	aggagUgaccaaaagacaagagUgcgagccUUcUaUUaUgcccag
  			4448		acagggccaccagagGGCUCCUUGGUCUAGGGGUAaUgcca
  853	CGUCCCGGGGCUGCGCGAGGCA	hsa-mir-2288	hsa-mir-	1147	agcagcccUcggcggcccggggggcgggcggcggUgccCGUCCCG
  			4449		GGGCUGCGCGAGGCAcaggcg
  854	AAAGGUAAUUGUGGUUUCUGC	hsa-mir-2289-	hsa-mir-	1148	gUgcAAAGGUAAUUGUGGUUUCUGCUUUUaaaggUaaU
  		1	548ag-1		ggcaaaUaUUacaUUUacUUUUgcacca
  855	AAAGGUAAUUGUGGUUUCUGC	hsa-mir-2289-	hsa-mir-	1149	UgcAAAGGUAAUUGUGGUUUCUGCcaUUgaaagUaaagg
  		2	548ag-2		caagaaccUcaaUUaccUUUgcagc
  856	UGGGGAUUUGGAGAAGUGGUGA	hsa-mir-2290	hsa-mir-	1150	UgUcUGGGGAUUUGGAGAAGUGGUGAgcgcaggUcUUU
  			4450		ggcaccaUcUccccUggUcccUUggcU
  857	AAAAGUGAUUGCAGUGUUUG	hsa-mir-2291	hsa-mir-	1151	aggUUggUgcAAAAGUGAUUGCAGUGUUUGccaaUaaaa
  			548ah		gUaaUgacaaaaacUgcagUUacUUUUgcaccagccc
  858	UGGUAGAGCUGAGGACA	hsa-mir-2292	hsa-mir-	1152	UcUgUaccUcagcUUUgcUcccaaccaaccacUUccacaUgUU
  			4451		UUgcUGGUAGAGCUGAGGACAgc
  859	UUGAAUUCUUGGCCUUAAGUGAU	hsa-mir-2293	hsa-mir-	1153	UggaUcacUUgaggccaagagUgcaaggcUgUagUgUgcacagc
  			4452		cUUGAAUUCUUGGCCUUAAGUGAUccc
  860	UAGGAGCUCAACAGAUGCCUGU	hsa-mir-2294	hsa-mir-	1154	UcagagUAGGAGCUCAACAGAUGCCUGUUgacUgaaUaa
  			3139		UaaacaggUaUcgcaggagcUUUUgUUaUgU
  861	AGCUUUUGGGAAUUCAGGUAG	hsa-mir-2295	hsa-mir-	1155	UgUccUcUUgaggUaccUgaaUUaccaaaagcUUUaUgUaUU
  			3140		cUgaagUUaUUgaaaaUaagAGCUUUUGGGAAUUCAGGU
  					AGUUcaggagUgacU
  862	GAGCUUGGUCUGUAGCGGUU	hsa-mir-2296	hsa-mir-	1156	UggaGAGCUUGGUCUGUAGCGGUUUccUUggggcaggUg
  			4453		gggacUgcUccUUUgggaggaaggaggaggcccaggccgcgUcUU
  					cagg
  863	GGAUCCGAGUCACGGCACCA	hsa-mir-2297	hsa-mir-	1157	ccGGAUCCGAGUCACGGCACCAaaUUUcaUgcgUgUccgU
  			4454		gUgaagagaccacca
  864	CAAAAGUGAUCGUGGUUUUUG	hsa-mir-2298	hsa-mir-	1158	gUggUgCAAAAGUGAUCGUGGUUUUUGcaaUUUUUUaa
  			548t		UgacaaaaaccacaaUUacUUUUgcaccaa
  865	AGGGUGUGUGUGUUUUU	hsa-mir-2299	hsa-mir-	1159	agaAGGGUGUGUGUGUUUUUccUgagaaUaagagaaggaa
  			4455		ggacagccaaaUUcUUca
  866	CCUGGUGGCUUCCUUUU	hsa-mir-2300	hsa-mir-	1160	aUgaaCCUGGUGGCUUCCUUUUcUgggaggaagUUagggU
  			4456		Uca
  867	UCACAAGGUAUUGACUGGCGUA	hsa-mir-2301	hsa-mir-	1161	ggagUacUccagUcaaUaccgUgUgagUUagaaaagcUcaaUU
  			4457		CACAAGGUAUUGACUGGCGUAUUca
  868	AGAGGUAGGUGUGGAAGAA	hsa-mir-2302	hsa-mir-	1162	gagcgcacAGAGGUAGGUGUGGAAGAAagUgaaacacUaU
  			4458		UUUaggUUUUagUUacacUcUgcUgUggUgUgcUg
  869	CCAGGAGGCGGAGGAGGUGGAG	hsa-mir-2303	hsa-mir-	1163	acCCAGGAGGCGGAGGAGGUGGAGgUUgcagUgagccaag
  			4459		aUcgUggcacUgacUccagccUgggg
  870	GUGGAGGACUGAGAAGGUGAG	hsa-mir-2304	pending	1164	aUgaUGUGGAGGACUGAGAAGGUGAGgcagUUUUgcccc
  					gUgcUgccUUccaccggUUaagaccUccaaaaUcga
  871	ACUGACAGGAGAGCAUUUUGA	hsa-mir-2305	hsa-mir-	1165	ggacaaaaUUaaaaUgcUcUUcUgUcaUUgUaaUagUUcaUa
  			3660		UgggcACUGACAGGAGAGCAUUUUGAcUUUgUca
  872	AGCGCGGGCUGAGCGCUGCCAG	hsa-mir-2306	hsa-mir-	1166	ggUUcacUggUcgUgcUUccUgcgggcUgAGCGCGGGCUGA
  			2277		GCGCUGCCAGUcagcg
  873	AAGCUCGGGCGCUCCGGCUGU	hsa-mir-2307	pending	1167	gcUcagUcagcUgggccgccUcagcUcUcggagUaggAAGCUCG
  					GGCGCUCCGGCUGUaaggagcc
  874	AUAGUGGUUGUGAAUUUACCUU	hsa-mir-2308	hsa-mir-	1168	gUUUUUUgcccAUAGUGGUUGUGAAUUUACCUUcUccU
  			4460		cUUUgcagUgaUaaaggaggUaaaUUcacaaccacUgUgggcag
  					aaac
  875	GAUUGAGACUAGUAGGGCUAGGC	hsa-mir-2309	hsa-mir-	1169	gagUaggcUUaggUUaUgUacgUagUcUaggccaUacgUgUU
  			4461		ggaGAUUGAGACUAGUAGGGCUAGGCcUacUg
  876	CUACCCCAGGAUGCCAGCAUAG	hsa-mir-2310	pending	1171	aUagcUggUUggcaUUcUggcccUggUUcaUgccaacUcUUg
  					UgUUgaCUACCCCAGGAUGCCAGCAUAGUUgc
  877	ACUGGACUUGGUGUCAGAUGG	hsa-mir-2311	hsa-mir-	1171	acaggaacACUGGACUUGGUGUCAGAUGGgaUgagcccUg
  			378h		gcUcUgUUUccUagcagcaaUcUgaUcUUgagcUagUcacUgg
  878	GACAAUGAUGAGAAGACCUGAG	hsa-mir-2312	pending	1172	gggGACAAUGAUGAGAAGACCUGAGgaUUUgcagccccca
  					gcccUgggUUcaagUcccagcUcUaccccUUcUUggcccc
  879	ACCGCUCGAUCUUGGGACC	hsa-mir-2313	pending	1173	gUUUcACCGCUCGAUCUUGGGACCcaccgcUgcccUcagcU
  					ccgagUccagggcgaggUaagggcUggagUcgggcagga
  880	UAGUGGAUGAUGGAGACUCGGU	hsa-mir-2314	hsa-mir-	1174	aUUgaggcacUgggUAGUGGAUGAUGGAGACUCGGUacc
  			3691		cacUgcUgagggUggggaccaagUcUgcgUcaUccUcUccUcag
  					UgccUcaaa
  881	CUCGGGAGCGUUAGAGAUGGA	hsa-mir-2315	pending	1175	gUCUCGGGAGCGUUAGAGAUGGAgacUaacgUcUUccaa
  					gggagaUUgcgUcUccacUUUcacccUggUacUgagag
  882	GGCUGGAGCGAGUGCAGUGGUG	hsa-mir-2316	hsa-mir-	1176	UgcccaGGCUGGAGCGAGUGCAGUGGUGcagUcagUccU
  			3135b		agcUcacUgcagccUcgaacUccUgggcU
  883	UCAGGUGUGGAAACUGAGGCAG	hsa-mir-2317	hsa-mir-	1177	UUUUCAGGUGUGGAAACUGAGGCAGgaggcagUgaagUa
  			3934		acUUgcUcaggUUgcacagcUgggaagU
  884	UGACACGGAGGGUGGCUUGGGAA	hsa-mir-2318	hsa-mir-	1178	cUUcccagcUgcccUaagUcaggagUggcUUUccUGACACGG
  			4462		AGGGUGGCUUGGGAAa
  885	GAAGAUGGUGCUGUGCUGAGGA	hsa-mir-2319	pending	1179	ggUGAAGAUGGUGCUGUGCUGAGGAaaggggaUgcagagc
  					ccUgcccagcaccaccaccUccUaUg
  886	AAAGACUGCAAUUACUUUUGCG	hsa-mir-2320	hsa-mir-	1180	aUggUgcaaaagUaaUgUggUUUUUUUcUUUacUUUUaaU
  			548u		ggcAAAGACUGCAAUUACUUUUGCGcca
  887	GAGACUGGGGUGGGGCC	hsa-mir-2321	hsa-mir-	1181	aaUagaUUaUUggUcaccaccUccagUUUcUgaaUUUgUGA
  			4463		GACUGGGGUGGGGCCUgagaaUUUgc
  888	AAGGUUUGGAUAGAUGCAAUA	hsa-mir-2322	hsa-mir-	1182	ggaaccUUagUAAGGUUUGGAUAGAUGCAAUAaagUaUg
  			4464		UccacagcUgaaaggacaUacUUUaUUgcaUgUaUccaaaccU
  					UacUaaUUca
  889	AAAGGUAAUUGCAGUUUUUCCC	hsa-mir-2323	hsa-mir-	1183	gUaUUaggUUggUgcAAAGGUAAUUGCAGUUUUUCCCa
  			548ai		UUUaaaaUaUggaaaaaaaaaUcacaaUUacUUUUgcaUcaa
  					ccUaaUaa
  890	AGGGGACCAAAGAGAUAUAUAG	hsa-mir-2324	hsa-mir-	1184	gaaacUacacUUUaAGGGGACCAAAGAGAUAUAUAGaUa
  			3144		UcagcUaccUaUaUaccUgUUcggUcUcUUUaaagUgUagU
  					UUa
  891	UAAAAACUGCAAUUACUUUUA	hsa-mir-2325-	hsa-mir-	1185	aUUggUgUaaaagUaaUUgcaggUUaUgccaUUaaaagUaaU
  		1	548aj-1		ggUAAAAACUGCAAUUACUUUUAcacUaac
  892	UAAAAACUGCAAUUACUUUUA	hsa-mir-2325-	hsa-mir-	1186	aaggUaUUaggUUggUgcaaaagUaaUUgcagUUUUUgcUa
  		2	548aj-2		UUacUUUUaaUggUAAAAACUGCAAUUACUUUUAcacca
  					accUaaUaUUUa
  893	CUCAAGUAGUCUGACCAGGGGA	hsa-mir-2326	hsa-mir-	1187	caUgUgUccccUggcacgcUaUUUgaggUUUacUaUggaacC
  			4465		UCAAGUAGUCUGACCAGGGGAcacaUga
  894	UCUGGCUGAGGAGGAAGUGGAG	hsa-mir-2327-	pending	1188	gcUcUagUagccacagccaUccccUagagggaUCUGGCUGAGG
  		1			AGGAAGUGGAGg
  895	UCUGGCUGAGGAGGAAGUGGAG	hsa-mir-2327-	pending	1189	ccgccUcagUggcUUccUccacagccaccUccggagggaUCUGGC
  		2			UGAGGAGGAAGUGGAGgUgUcacUgg
  896	GGCGACAAAACGAGACCCUGU	hsa-mir-2328	hsa-mir-	1190	cUgGGCGACAAAACGAGACCCUGUcUUUUUUUUUUUc
  			1273c		UgagacagagUcUcgUUcUgUUgcccaa
  897	GGGUGCGGGCCGGCGGGG	hsa-mir-2329	hsa-mir-	1191	acgcGGGUGCGGGCCGGCGGGGUagaagccacccggcccggc
  			4466		ccggcccggcga
  898	CCUGCUGGUCAGGAGUGGAUAC	hsa-mir-2330	hsa-mir-	1192	gccaUUCCUGCUGGUCAGGAGUGGAUACUggagcaaUag
  			3692		aUacagUUccacacUgacacUgcagaagUgga
  899	CAUGCUAGGAUAGAAAGAAUGG	hsa-mir-2331	hsa-mir-	1193	aUUUUcUUUgcUaagUcccUUcUUUcUaUccUagUaUaac
  			3146		UUgaagaaUUcaaaUagUCAUGCUAGGAUAGAAAGAAUG
  					GgacUUggccagggaagaa
  900	AAUGUGGAAGUGGUCUGAGGCA	hsa-mir-2332	pending	1194	gaaUagaaagAAUGUGGAAGUGGUCUGAGGCAUaUagag
  					UaUaUgccaagaacacUaccaUa
  901	GCAAAGUGAUGAGUAAUACUGG	hsa-mir-2333	hsa-mir-	1195	acagUaacUUUUaUUcUcaUUUUccUUUUcUcUaccUUgU
  			3609		agagaaGCAAAGUGAUGAGUAAUACUGGcUgg
  902	UGGCGGCGGUAGUUAUGGGCUU	hsa-mir-2334	hsa-mir-	1196	UggUGGCGGCGGUAGUUAUGGGCUUcUcUUUcUcacca
  			4467		gcagccccUgggccgccgccUcccU
  903	AGAGCAGAAGGAUGAGAU	hsa-mir-2335	hsa-mir-	1197	agUcUUcUccUggggcUUUggUggcUaUggUUgacUgggccac
  			4468		UcAGAGCAGAAGGAUGAGAUg
  904	UGGAAUGGCCUGAAGGUGGA	hsa-mir-2336	pending	1198	ccUggcagcccUcUggccUagUUcccaccacacaUgaggUggUG
  					GAAUGGCCUGAAGGUGGAacaga
  905	GCUCCCUCUAGGGUCGCUCGGA	hsa-mir-2337	hsa-mir-	1199	ccgacgcggagagcggcUcUaggUgggUUUggcggcggcgaggaca
  			4469		ccgccGCUCCCUCUAGGGUCGCUCGGAgcgUga
  906	UGGCAAACGUGGAAGCCGAGA	hsa-mir-2338	hsa-mir-	1200	cgagccUcUUUcggcUUUccagUUUgUcUcggUccUUUggaa
  			4470		cgUGGCAAACGUGGAAGCCGAGAgggcUcU
  907	UUUGUAUGGAUAUGUGUGUGUA	hsa-mir-2339	hsa-mir-	1201	aUaUacaUacaUgUacacacacaUgUcaUccacacacaUacaUa
  			3149		UaUaUaUgUUUGUAUGGAUAUGUGUGUGUAUgUgUg
  					UgUaUac
  908	UGAGGAGAUCGUCGAGGUUGGC	hsa-mir-2340	hsa-mir-	1202	aaagcaggccaaccUcgaggaUcUccccagccUUggcgUUcaggU
  			3150b		gcUGAGGAGAUCGUCGAGGUUGGCcUgcUUc
  909	UGGGAACUUAGUAGAGGUUUAA	hsa-mir-2341	hsa-mir-	1203	ccaaaUUUaaaacUUaaaccUcUacUaagUUUccaUgaaaaga
  			4471		acccaUGGGAACUUAGUAGAGGUUUAAgUUUUaaaUUU
  					ga
  910	GGUGGGGGGUGUUGUUUU	hsa-mir-2342-	hsa-mir-	1204	UggcagacccUUgcUcUcUcacUcUcccUaaUggggcUgaagac
  		1	4472-1		agcUcaggggcagGGUGGGGGGUGUUGUUUUUgUUU
  911	GGUGGGGGGUGUUGUUUU	hsa-mir-2342-	hsa-mir-	1205	UggUgggGGUGGGGGGUGUUGUUUUUgUUUUUgagaca
  		2	4472-2		gagUcUUgcUccgUcgcccaggccggagU
  912	CUAGUGCUCUCCGUUACAAGUA	hsa-mir-2343	hsa-mir-	1206	aaggaacaggggacacUUgUaaUggagaacacUaagcUaUggac
  			4473		UgcUaUggacUgCUAGUGCUCUCCGUUACAAGUAUcccc
  					UgUUaccU
  913	CACUUGUAAUGGAGAACACUAA	hsa-mir-2344	pending	1207	aaaggaacaggggaCACUUGUAAUGGAGAACACUAAgcUa
  					UggacUgcUaUggacUgcUagUgcUcUccgUUacaagUaUccc
  					cUgUUaccUUg
  914	UUGUGGCUGGUCAUGAGGCUAA	hsa-mir-2345	hsa-mir-	1208	UUgccUaccUUgUUagUcUcaUgaUcagacacaaaUaUggcUc
  			4474		UUUGUGGCUGGUCAUGAGGCUAAcaaggUaggcac
  915	CCCUGGGGUUCUGAGGACAUG	hsa-mir-2346	pending	1209	aUgcUgCCCUGGGGUUCUGAGGACAUGcUcUgacUcccc
  					UgaUgUccUcUgUUccUcaggUgcUgggcga
  916	CAAGGGACCAAGCAUUCAUUAU	hsa-mir-2347	hsa-mir-	1210	aUcUcaaUgagUgUgUggUUcUaaaUgacUcaUagUCAAGG
  			4475		GACCAAGCAUUCAUUAUgaa
  917	CAGGAAGGAUUUAGGGACAGGC	hsa-mir-2348	hsa-mir-	1211	aaaagccUgUcccUaagUcccUcccagccUUccagagUUggUgc
  			4476		CAGGAAGGAUUUAGGGACAGGCUUUg
  918	CUAUUAAGGACAUUUGUGAUUC	hsa-mir-2349	hsa-mir-	1212	UccUccUcccaUcaaUcacaaaUgUccUUaaUggcaUUUaagg
  			4477a		aUUgCUAUUAAGGACAUUUGUGAUUCacgggaggaggU
  919	AUUAAGGACAUUUGUGAUUGAU	hsa-mir-2350	hsa-mir-	1213	accUccUcccgUgaaUcacaaaUgUccUUaaUagcaaUccUUaa
  			4477b		aUgccAUUAAGGACAUUUGUGAUUGAUgggaggagga
  920	AAAAGGCAUAAAACCAAGACA	hsa-mir-2351-	hsa-mir-	1214	cUUUUUUUgUUgcUUgUcUUggUUUUaUgccUUUUaUg
  		1	3910-2		UgccUUgaUaUAAAAGGCAUAAAACCAAGACAagcaacag
  					aaaaac
  921	AAAAGGCAUAAAACCAAGACA	hsa-mir-2351-	hsa-mir-	1215	cUgUcagUUUUUcUgUUgcUUgUcUUggUUUUaUgccUU
  		2	3910-1		UUaUaUcaaggcacaUAAAAGGCAUAAAACCAAGACAagc
  					aacaa
  922	GAGGCUGAGCUGAGGAG	hsa-mir-2352	hsa-mir-	1216	ggccGAGGCUGAGCUGAGGAGccUccaaaccUgUagacagg
  			4478		gUcaUgcagUacUaggggcgagccUcaUccccUgcagcccUggcc
  923	CUGGGAGGUGUGAUAUUGUGGU	hsa-mir-2353	hsa-mir-	1217	gggaggUgUgaUaUcgUggUUccUgggaggUgUgaUaUcgUg
  			3689c		gUUcCUGGGAGGUGUGAUAUUGUGGUUccU
  924	UAAAAACUGCAAUUACUUUC	hsa-mir-2354-	hsa-mir-	1218	aUgccaaaUaUUaggUUggcacaaaagUaaUUgUggcUUUUg
  		1	548x-2		ccaUUaaaagUaaUggUAAAAACUGCAAUUACUUUCgUgc
  					caaccUaaUaUUUgUgUg
  925	UAAAAACUGCAAUUACUUUC	hsa-mir-2354-	hsa-mir-	1219	agUgcaaaagUaaUUgcagUUUUUgcgUUacUUUcaaUcgU
  		2	548x-1		AAAAACUGCAAUUACUUUCacacc
  926	UGUGAUAUCAUGGUUCCUGGGA	hsa-mir-2355-	hsa-mir-	1220	gcUcccUgggaggUGUGAUAUCAUGGUUCCUGGGAggUg
  		1	3689a		UgaUccUgUgcUUccUgggaggUgUgaUaUcgUggUUccUgg
  					gagg
  927	GGGAGGUGUGAUCUCACACUCG	has-mir-2356-	hsa-mir-	1221	UGGGAGGUGUGAUCUCACACUCGcUgggaggUgUgcUaU
  		1	3689d-1		cgUcUUccccgggaggUgUgaUccUgUUcUUccUg
  928	CUGGGAGGUGUGAUAUUGUGGU	mir-2355-2*	has-mir-	1222	gggaggUGUGAUAUCAUGGUUCCUGGGAggUgUgaUccc
  			3689b-1		gUgcUUcCUGGGAGGUGUGAUAUUGUGGUUccU
  929	UGUGAUAUCAUGGUUCCUGGGA	hsa-mir-2355-	hsa-mir-	1223	gggaggUGUGAUAUCAUGGUUCCUGGGAggUgUgaUccc
  		2	3689b-1		gUgcUUcCUGGGAGGUGUGAUAUUGUGGUUccU
  930	GGGAGGUGUGAUCUCACACUCG	has-mir-2356-	hsa-mir-	1224	acUGGGAGGUGUGAUCUCACACUCGcUgggaggUgUgcU
  		2	3689d-2		aUcgUcUUcccUgggaggUgUgaUccUgUUcUUccUgagcg
  931	UGUGAUAUCAUGGUUCCUGGGA	hsa-mir-2355-	has-mir-	1225	gggaggUGUGAUAUCAUGGUUCCUGGGAggUaUgaUaUc
  		3	3689b-2		gUggUUccUgggaggUgUgaUcccgUgcUcccU
  932	UGUGAUAUCGUGCUUCCUGGGA	hsa-mir-2355b	hsa-mir-	1226	aggUGUGAUAUCGUGCUUCCUGGGAcgUgUgaUgcUgUg
  			3689f		cUUccUgggaggUgUgaUcccacacUc
  933	CGCGCGGCCGUGCUCGGAGCAG	hsa-mir-2356	hsa-mir-	1227	gaaaccaagUccgagcgUggcUggcgcgggaaagUUcgggaacgC
  			4479		GCGCGGCCGUGCUCGGAGCAGcgcca
  934	CCAGGCUCUGCAGUGGGAACU	hsa-mir-2357a	hsa-mir-	1228	ccUgUUccgggcaUcaccUcccacUgcagagccUggggagccggac
  			3155a		agcUcccUUcCCAGGCUCUGCAGUGGGAACUgaUgccUgg
  					aacagU
  935	CCAGGCUCUGCAGUGGGA	hsa-mir-2357b	hsa-mir-	1229	ccacUgcagagccUgggaagggagcUgUccggcUccCCAGGCUC
  			3155b		UGCAGUGGGAgU
  936	AAAAGUAACUGCGGUUUUUGA	hsa-mir-2358	hsa-mir-	1230	gUgcAAAAGUAACUGCGGUUUUUGAgaagUaaUUgaaaa
  			548ak		ccgcaaUUacUUUUgcag
  937	AGCCAAGUGGAAGUUACUUUA	hsa-mir-2359	hsa-mir-	1231	gcagaggUgagUUgaccUccacagggccacccagggagUaagUAG
  			4480		CCAAGUGGAAGUUACUUUAccUcUgU
  938	GGAGUGGGCUGGUGGUU	hsa-mir-2360	hsa-mir-	1232	GGAGUGGGCUGGUGGUUUUUUaagaggaagggagaccUa
  			4481		agcUagcacaUgagcacgcUc
  939	GAGCGAUCCGAGGGACUG	hsa-mir-2361	pending	1233	cggcUUcccgcggUccccggUgcUgaggagaGAGCGAUCCGAG
  					GGACUGcgccgcc
  940	AAGGGCUUCCUCUCUGCAGGAC	hsa-mir-2362-	hsa-mir-	1234	acaUUaUUcaggccggUccUgcagagaggaagcccUUccaaUacc
  		1	3158-2		UgUaagcagAAGGGCUUCCUCUCUGCAGGACcggccUgaa
  					UaaUga
  941	AAGGGCUUCCUCUCUGCAGGAC	hsa-mir-2362-	hsa-mir-	1235	ggaUcaUUaUUcaggccggUccUgcagagaggaagcccUUcUgc
  		2	3158-1		UUacaggUaUUggAAGGGCUUCCUCUCUGCAGGACcggcc
  					UgaaUaaUgUaaUca
  942	AACCCAGUGGGCUAUGGAAAUG	hsa-mir-2363	hsa-mir-	1236	agUgagcAACCCAGUGGGCUAUGGAAAUGUgUggaagaU
  			4482		ggcaUUUcUaUUUcUcagUggggcUcUUacc
  943	GGGGUGGUCUGUUGUUG	hsa-mir-2364	hsa-mir-	1237	aaaaaacaacaUacUUagUgcaUacccaUaUaaUaUUaGGGG
  			4483		UGGUCUGUUGUUGUUUUUcU
  944	AAAAGGCGGGAGAAGCCCCA	hsa-mir-2365	hsa-mir-	1238	gggUUUccUcUgccUUUUUUUccaaUgaaaaUaacgaaaccU
  			4484		gUUaUUUcccaUUgagggggaAAAAGGCGGGAGAAGCCCC
  					A
  945	UGGCUGUUGGAGGGGGCAGGCU	hsa-mir-2366	pending	1239	ggagccagcccUccUcccgcacccaaacUUggagcacUUgaccUU
  					UGGCUGUUGGAGGGGGCAGGCUcg
  946	UAACGGCCGCGGUACCCUAA	hsa-mir-2367	hsa-mir-	1240	agaggcaccgccUgcccagUgacaUgcgUUUAACGGCCGCGG
  			4485		UACCCUAAcUgUgca
  947	UAGGAUUACAAGUGUCGGCCAC	hsa-mir-2368	hsa-mir-	1241	ccUAGGAUUACAAGUGUCGGCCACgggcUgggcacagUggc
  			3159		UcacgccUgUaaUcccagc
  948	GCUGGGCGAGGCUGGCA	hsa-mir-2369	hsa-mir-	1242	gcaUGCUGGGCGAGGCUGGCAUcUagcacaggcggUagaUg
  			4486		cUUgcUcUUgccaUUgcaaUga
  949	AGAGCUGGCUGAAGGGCAG	hsa-mir-2370	hsa-mir-	1243	acUgUccUUcagccAGAGCUGGCUGAAGGGCAGaagggaac
  			4487		UgUccUUcagccagagcUggcUgaagggcaga
  950	AGGGGGCGGGCUCCGGCG	hsa-mir-2371	hsa-mir-	1244	ggUAGGGGGCGGGCUCCGGCGcUgggaccccacUagggUgg
  			4488		cgccUUggccccgccccgccc
  951	GCCGAGAGUCGUCGGGGUU	hsa-mir-2372	pending	1245	UcGCCGAGAGUCGUCGGGGUUUccUgcUUcaacagUgcU
  					Uggacggaacccggcgc
  952	UGGGGCUAGUGAUGCAGGACG	hsa-mir-2373	hsa-mir-	1246	gggggUGGGGCUAGUGAUGCAGGACGcUggggacUggaga
  			4489		agUccUgccUgacccUgUccca
  953	UGUGACUUUAAGGGAAAUGGCG	hsa-mir-2374	hsa-mir-	1247	UggaaacUGUGACUUUAAGGGAAAUGGCGcacagcagacc
  			3164		cUgcaaUcaUgccgUUUUgcUUgaagUcgcagUUUccc
  954	UCUCAGGAGUAAAGACAGAGUU	hsa-mir-2375	hsa-mir-	1248	aacUUgaaggUagggaacUcUgUcUUcacUcaUgagUaccUUc
  			3664		caacacgagcUCUCAGGAGUAAAGACAGAGUUcccUaccU
  					UcaaUgU
  955	AGGUGGAUGCAAUGUGACCUCA	hsa-mir-2376	hsa-mir-	1249	caAGGUGGAUGCAAUGUGACCUCAacUcUUggUccUcUg
  			3165		aggUcacaUUgUaUccaccUUa
  956	AACGGCAAUGACUUUUGUACCA	hsa-mir-2377	hsa-mir-	1250	ggUcggUgcaaaagUaaUUgcUgUUUUUgccaUUaaaaaUaa
  			548a1		UggcaUUaaaagUaaUggcaaaAACGGCAAUGACUUUUGU
  					ACCAaUcUaaUaUcU
  957	UCUGGUAAGAGAUUUGGGCAUA	hsa-mir-2378	hsa-mir-	1251	aUagUUUcUgcaaUgcUcaaaUcUcUggccaaagaccagaacU
  			4490		UaaUggUcUCUGGUAAGAGAUUUGGGCAUAUUagaaac
  					Uaa
  958	AAUGUGGACUGGUGUGACCAAA	hsa-mir-2379-	hsa-mir-	1252	acaUUUggUcacaccagUccacaUUaacgUggaccagacaaUaU
  		1	4491		UAAUGUGGACUGGUGUGACCAAAa
  959	AAUGUGGACUGGUGUGACCAAA	hsa-mir-2379-	pending	1253	accUggacaUUUggUcacaccagUccacaUUaacgUggaccagac
  		2			aaUaUUAAUGUGGACUGGUGUGACCAAAagUccaggc
  960	GGGGCUGGGCGCGCGCC	hsa-mir-2380	hsa-mir-	1254	cUgcagcgUgcUUcUccaggccccgcgcgcggacagacacacgga
  			4492		caagUcccgccaGGGGCUGGGCGCGCGCCagccgg
  961	AGAAGGCCUUUCCAUCUCUGU	hsa-mir-2381	hsa-mir-	1255	ccagagaUgggaaggccUUccggUgaUUaUcacagccaUgccUU
  			4493		UaccUccAGAAGGCCUUUCCAUCUCUGUc
  962	CCAGACUGUGGCUGACCAGAGG	hsa-mir-2382	hsa-mir-	1256	agUUUUagUUacccUggUcaUcUgcagUcUgaaaaUacaaaa
  			4494		UggaaaaUUCCAGACUGUGGCUGACCAGAGGUaacUgaa
  					acc
  963	AGGAGAAGCAGGAGCUGU	hsa-mir-2383	pending	1257	cUgAGGAGAAGCAGGAGCUGUcUUggUacaUUcaggUcac
  					Ug
  964	AAUGUAAACAGGCUUUUUGCU	hsa-mir-2384	hsa-mir-	1258	aagaAAUGUAAACAGGCUUUUUGCUcagUggagUUaUUU
  			4495		UgagcaaaaagcUUaUUUacaUUUcUg
  965	GAGGAAACUGAAGCUGAGAGGG	hsa-mir-2385	hsa-mir-	1259	ACAUCAGCUCAUAUAAUCCUCGAAGCUGCCUUUAGAA
  			4496		AUGAGGAAACUGAAGCUGAGAGGG
  966	CUCCGGGACGGCUGGGC	hsa-mir-2386	hsa-mir-	1260	acCUCCGGGACGGCUGGGCgccggcggccgggagaUccgcgcU
  			4497		UccUgaaUcccggccggcccgcccggcgcccgUccgcccgcgggUc
  967	UGGGCUGGCAGGGCAAGUGCUG	hsa-mir-2387	hsa-mir-	1261	agggcUGGGCUGGCAGGGCAAGUGCUGcagaUcUUUgUc
  			4498		UaagcagccccUgccUUggaUcUccca
  968	GAGGCUGAAGGAAGAUGG	hsa-mir-2388	hsa-mir-	1262	cUcaggcUcagUggUgcaUgcUUaUagUcccagccacUcUgGA
  			4419b		GGCUGAAGGAAGAUGGcUUgagccU
  969	AAGACUGAGAGGAGGGA	hsa-mir-2389	hsa-mir-	1263	AAGACUGAGAGGAGGGAacUggUgagUUgUacaUagaaa
  			4499		UgcUUUcUaacUccUUgUcUcagUcUgUUU
  970	UGAGGUAGUAGUUUCUU	hsa-mir-2390	hsa-mir-	1264	caggagagaaagUacUgcccagaagcUaaagUgUagaUcaaacgc
  			4500		aUaaUggcUGAGGUAGUAGUUUCUUgaacUU
  971	CGGCUGGGAGCCGAGGCGUCGG	hsa-mir-2391	pending	1265	ggCGGCUGGGAGCCGAGGCGUCGGUgcagaccUggagacg
  					ggcaUgggggggcUgcggcUgcUggcUgUg
  972	UAUGUGACCUCGGAUGAAUCA	hsa-mir-2392	hsa-mir-	1266	UAUGUGACCUCGGAUGAAUCAcUgaaaUaUgUcUgagcU
  			4501		UcUgUUUcaUcagaUgUcacaUUUU
  					agccUUUagcaagUUgUaaUcUUUUUgcUgaUggagggUcU
  973	GCUGAUGAUGAUGGUGCUGAAG	hsa-mir-2393	hsa-mir-	1267	UgccUccaUggggaUgGCUGAUGAUGAUGGUGCUGAAGg
  			4502		c
  974	AGAUGUAUGGAAUCUGUAUAU	hsa-mir-2394	hsa-mir-	1268	gAGAUGUAUGGAAUCUGUAUAUaUcUaUaUaUaUgUgU
  			3171		aUaUaUagaUUccaUaaaUcUa
  975	UUUAAGCAGGAAAUAGAAUUUA	hsa-mir-2395	hsa-mir-	1269	acaaUgUagaUaUUUAAGCAGGAAAUAGAAUUUAcaUaU
  			4503		aaaUUUcUaUUUgUUUcUaUUUccUgcUUaaaUaUcUaca
  					UUgc
  976	UGUGACAAUAGAGAUGAACAUG	hsa-mir-2396	hsa-mir-	1270	cUaagaUaaUgUccUccaggUUcaUcUcUgUUgUcaUUUgU
  			4504		ggcaUggaccaUUUGUGACAAUAGAGAUGAACAUGgagga
  					UaUUaUcUUaa
  977	AGGCUGGGCUGGGACGGA	hsa-mir-2397	hsa-mir-	1271	ggAGGCUGGGCUGGGACGGAcacccggccUccacUUUcUg
  			4505		UggcaggUaccUccUccaUgUcggcccgccUUg
  978	AAAUGGGUGGUCUGAGGCAA	hsa-mir-2398	hsa-mir-	1272	UggccUcUgccaUcagaccaUcUgggUUcaagUUUggcUccaU
  			4506		cUUUaUgAAAUGGGUGGUCUGAGGCAAgUggUcU
  979	UAGGAUGGGGGUGAGAGGUG	hsa-mir-2399	hsa-mir-	1273	aUggUcccUcccaaUccagccaUUccUcagaccaggUggcUcccg
  			2392		agccaccccaggcUgUAGGAUGGGGGUGAGAGGUGcUag
  980	CUGGGUUGGGCUGGGCUGGG	hsa-mir-2400	hsa-mir-	1274	UcUgggcUgagccgagcUgggUUaagccgagCUGGGUUGGGC
  			4507		UGGGCUGGGU
  981	AAGGGACUGGAGUGGAUUGGGU	hsa-mir-2401	pending	1275	ggAAGGGACUGGAGUGGAUUGGGUacaUcUaUUaUagU
  					gggagcaccUacUacaacccgUcccUcaagagUcgagUcacc
  982	AAGGGGCUGGAGUGGAUUGGGG	hsa-mir-2402	pending	1276	cUggUgaaUcUgggUccgccagcccccagggAAGGGGCUGGA
  					GUGGAUUGGGGaaaUccaU
  983	GCGGGGCUGGGCGCGCG	hsa-mir-2403	hsa-mir-	1277	aggacccaGCGGGGCUGGGCGCGCGgagcagcgcUgggUgca
  			4508		gcgccUgcgccggcagcUgcaagggccg
  984	ACUAAAGGAUAUAGAAGGUUUU	hsa-mir-2404-	hsa-mir-	1278	cUUUaaUacUaUcUcaaACUAAAGGAUAUAGAAGGUUU
  		1	4509-1		UcccUUUcUcUUgcccUgaaaccUUcUgUaUccUUUaUUUU
  					gagaUagUaUUagaa
  985	ACUAAAGGAUAUAGAAGGUUUU	hsa-mir-2404-	hsa-mir-	1279	cUUUaaUacUaUcUcaaACUAAAGGAUAUAGAAGGUUU
  		2	4509-2		UcccUUUcUcUUgcccUgaaaccUUcUgUaUccUUUaUUUU
  					gagaUagUaUUagaa
  986	ACUAAAGGAUAUAGAAGGUUUU	hsa-mir-2404-	hsa-mir-	1280	cUUUaaUacUaUcUcaaACUAAAGGAUAUAGAAGGUUU
  		3	4509-3		UcccUUUcUcUUgcccUgaaaccUUcUgUaUccUUUaUUUU
  					gagaUagUaUUagaa
  987	AAGCAAUACUGUUACCUGAAAU	hsa-mir-2405	hsa-mir-	1281	cUcaaagAAGCAAUACUGUUACCUGAAAUaggcUgcgaaga
  			3942		UaacagUaUUUcagaUaacagUaUUacaUcUUUgaa
  988	UGAGGGAGUAGGAUGUAUGGUU	hsa-mir-2406	hsa-mir-	1282	gUgUaUgUGAGGGAGUAGGAUGUAUGGUUgUUagaUa
  			4510		gacaacUacaaUcUUUUcUcacaacagacag
  989	GAAGAACUGUUGCAUUUGCCCU	hsa-mir-2407	hsa-mir-	1283	aaaaaaaagggaaaGAAGAACUGUUGCAUUUGCCCUgcac
  			4511		UcagUUUgcacagggUaaaUgcaaUagUUcUUcUUUcccUU
  					UUUUUa
  990	CAGGGCCUCACUGUAUCGCCCA	hsa-mir-2408	hsa-mir-	1284	cUcagcccgggcaaUaUagUgagaccUcgUcUcUacaaaaaaUU
  			4512		gagaCAGGGCCUCACUGUAUCGCCCAggcUgga
  					aUUcUaggUggggAGACUGACGGCUGGAGGCCCAUaagc
  991	AGACUGACGGCUGGAGGCCCAU	hsa-mir-2409	hsa-mir-	1285	UgUcUaaaacUUcggcccccagaUUUcUggUcUccccacUUcag
  			4513		aac
  992	ACAGGCAGGAUUGGGGAA	hsa-mir-2410	hsa-mir-	1286	gUUgagACAGGCAGGAUUGGGGAAacaUcUUUUaccUcg
  			4514		UcUcUUgccUgUUUUaga
  993	AGGACUGGACUCCCGGCAGCCC	hsa-mir-2411	hsa-mir-	1287	gcgggaggUgUaacAGGACUGGACUCCCGGCAGCCCcaggg
  			4515		caggggcgUggggagcUggUccUagcUcagcgcUcccgga
  994	UAGUGAGUUAGAGAUGCAGAGC	hsa-mir-2412	hsa-mir-	1288	agcgUUaccUggUAGUGAGUUAGAGAUGCAGAGCccUgg
  			3174		gcUccUcagcaaaccUacUggaUcUgca
  995	ACUGGCCUGGGACUACCGGGGG	hsa-mir-2413	hsa-mir-	1289	UcUgcagcUcccggcagccUcgggccacacUcccgggaUccccag
  			3176		ggACUGGCCUGGGACUACCGGGGGUggcggc
  996	GGGAGAAGGGUCGGGGC	hsa-mir-2414	hsa-mir-	1290	aGGGAGAAGGGUCGGGGCagggagggcagggcaggcUcUgg
  			4516		ggUggggggUcUgUgagUcagccacggcUcUgcccacgUcUcccc
  997	UGGGGCGGAGCUUCCGGAGGCC	hsa-mir-2415-	hsa-mir-	1291	agcUUccagacgcUccgccccacgUcgcaUgcgccccgggaacgc
  		1	3180-1		gUGGGGCGGAGCUUCCGGAGGCCcc
  998	UGGGGCGGAGCUUCCGGAGGCC	hsa-mir-2415-	hsa-mir-	1292	agcUUccagacgcUccgccccacgUcgcaUgcgccccgggaacgc
  		2	3180-2		gUGGGGCGGAGCUUCCGGAGGCCcc
  999	UGGGGCGGAGCUUCCGGAGGCC	hsa-mir-2415-	hsa-mir-	1293	agcUUccagacgcUccgccccacgUcgcaUgcgccccgggaacgc
  		3	3180-3		gUGGGGCGGAGCUUCCGGAGGCCcc
  1000	UGGGGCGGAGCUUCCGGAGGCC	hsa-mir-2415-	hsa-mir-	1294	agcUUccagacgcUccgccccacgUcgcaUgcgccccgggaacgc
  		4	3180-4		gUGGGGCGGAGCUUCCGGAGGCCcc
  1001	UGGGGCGGAGCUUCCGGAGGCC	hsa-mir-2415-	hsa-mir-	1295	agcUUccagacgcUccgccccacgUcgcaUgcgccccgggaacgc
  		5	3180-5		gUGGGGCGGAGCUUCCGGAGGCCcc
  1002	CUCGUGGGCUCUGGCCACGGC	hsa-mir-2416	hsa-mir-	1296	UggagggcaUUaggcagUggccagagcccUgcagUgcUgggcaUg
  			3677		ggcUUCUCGUGGGCUCUGGCCACGGCccUgagcUccUcc
  1003	AGAAGGGGUGAAAUUUAAACGU	hsa-mir-2417-	hsa-mir-	1297	gccaggaUcacagacgUUUaaaUUacacUccUUcUgcUgUgcc
  		1	3179-1		UUacagcagUAGAAGGGGUGAAAUUUAAACGUcUgUga
  					UccUggg
  1004	AGAAGGGGUGAAAUUUAAACGU	hsa-mir-2417-	hsa-mir-	1298	gaUcacagacgUUUaaaUUacacUccUUcUgcUgUgccUUaca
  		2	3179-2		gcagUAGAAGGGGUGAAAUUUAAACGUcUgUgaUccUgg
  					g
  1005	AGAAGGGGUGAAAUUUAAACGU	hsa-mir-2417-	hsa-mir-	1299	gccaggaUcacagacgUUUaaaUUacacUccUUcUgcUgUgcc
  		3	3179-3		UUacagcagUAGAAGGGGUGAAAUUUAAACGUcUgUga
  					UccUgg
  1006	AAAUAUGAUGAAACUCACAGCUG	hsa-mir-2418	hsa-mir-	1300	aggUAAAUAUGAUGAAACUCACAGCUGAGgagcUUagca
  	AG		4517		agUagcUaaggccagagcUUgUgUUUgggUggUgUggcUg
  1007	GCUCAGGGAUGAUAACUGUGCUG	hsa-mir-2419	hsa-mir-	1301	UgggggaaaagUgcUgggaUUgaUUagUgaUgUcUgcUgggga
  	AGA		4518		accgggGCUCAGGGAUGAUAACUGUGCUGAGAagcccccU
  1008	CAGCAGUGCGCAGGGCUG	hsa-mir-2420	hsa-mir-	1302	aaccUCAGCAGUGCGCAGGGCUGcacUgUcUccgUcUgcgg
  			4519		ccUgcagUaagcgggUa
  1009	UUGGACAGAAAACACGCAGGAA	hsa-mir-2421	hsa-mir-	1303	gUgUgccaccUgcgUgUUUUcUgUccaaaUcagaaaaggaUU
  			4520		UGGACAGAAAACACGCAGGAAgaaggaa
  1010	GCUAAGGAAGUCCUGUGCUCAG	hsa-mir-2422	hsa-mir-	1304	UcgGCUAAGGAAGUCCUGUGCUCAGUUUUgUagcaUca
  			4521		aaacUaggaUUUcUcUUgUUac
  1011	CUGGACUGAGCCAUGCUACUGG	hsa-mir-2423	hsa-mir-	1305	UgaggUUUCUGGACUGAGCCAUGCUACUGGcUUcUcUg
  			1269b		gUUcUccagcUUacagaUggcUUaUcaUgggaccUcU
  1012	UGACUCUGCCUGUAGGCCGGU	hsa-mir-2424	hsa-mir-	1306	gcgggcgUUgccUgggggccUcgcagggggagaUccagcccaggcU
  			4522		ggUUccgcUGACUCUGCCUGUAGGCCGGUggcgUcUUcU
  					gg
  1013	GACCGAGAGGGCCUCGGCUGU	hsa-mir-2425	hsa-mir-	1307	gcggggGACCGAGAGGGCCUCGGCUGUgUgaggacUagagg
  			4523		cggccgaggcccgggccggUUcccccga
  1014	UAGAGGCUGGAAUAGAGAUUCU	hsa-mir-2426	pending	1308	aUUUUAGAGGCUGGAAUAGAGAUUCUUgaggcUUggaa
  					gagUaaggaUcccUUUaUcUgUccUcUaggag
  1015	UAGCCUUCAGAUCUUGGUGUUU	hsa-mir-2427	hsa-mir-	1309	UggaUcUgaaggcUgccccUUUgcUcUcUggggUAGCCUUCA
  			3614		GAUCUUGGUGUUUU
  1016	CCACUUGGAUCUGAAGGCUGCC	hsa-mir-2427*	has-mir-	1310	UgggCCACUUGGAUCUGAAGGCUGCCccUUUgcUcUcUg
  			3614		gggUagccUUcagaUcUUggUgUUUU
  1017	AUAGCAGCAUGAACCUGUCUCA	hsa-mir-2428	hsa-mir-	1311	gaacgAUAGCAGCAUGAACCUGUCUCAcUgcagaaUUaUU
  			4524		UUgagacaggcUUaUgcUgcUaUccUUca
  1018	UGAGACAGGCUUAUGCUGCUAU	hsa-mir-2428*	has-mir-	1312	ggaacgaUagcagcaUgaaccUgUcUcacUgcagaaUUaUUUU
  			4524		GAGACAGGCUUAUGCUGCUAUccUUca
  1019	GGGGGGAUGUGCAUGCUGGUU	hsa-mir-2429	hsa-mir-	1313	gUcagaGGGGGGAUGUGCAUGCUGGUUggggUgggcUgc
  			4525		cUgUggaccaaUcagcgUgcacUUccccacccUgaa
  1020	CAGGGAGGUGAAUGGUUCUGUC	hsa-mir-2430	pending	1314	cUUagcUcccUggcUUcagcccUUUUUcCAGGGAGGUGAA
  					UGGUUCUGUCUcgc
  1021	GCUGACAGCAGGGCUGGCCGCU	hsa-mir-2431	hsa-mir-	1315	UgcggUgacaUcagggcccagUcccUgcUgUcaUgccccaggUga
  			4526		cgUgcUggGCUGACAGCAGGGCUGGCCGCUaacgUcacUg
  					Uc
  1022	UGGUCUGCAAAGAGAUGACUGU	hsa-mir-2432	hsa-mir-	1316	ccagaagUGGUCUGCAAAGAGAUGACUGUgaaUccaagaU
  			4527		ccacaUcagcUcUgUgcUgccUacaUcUga
  1023	UCAUUAUAUGUAUGAUCUGGAC	hsa-mir-2433	hsa-mir-	1317	UaUUcUacUgagagUacagaUcUUUaUaUaUaUgaUcaUUa
  			4528		UaUgUaUgaUgagaUCAUUAUAUGUAUGAUCUGGACacc
  					cagUagaaUc
  1024	AUUGGACUGCUGAUGGCCCGU	hsa-mir-2434	hsa-mir-	1318	aUgacaggccaUcagcagUccaaUgaagacaUgaagacccaaUgU
  			4529		cUUcAUUGGACUGCUGAUGGCCCGUcacUggga
  1025	UUGGAGGGUGUGGAAGACAUC	hsa-mir-2435	pending	1319	cUccacaUUGGAGGGUGUGGAAGACAUCUgggccaacUcU
  					gaUcUcUUcaUcUaccccccaggacUggga
  1026	CCCAGCAGGACGGGAGCG	hsa-mir-2436	hsa-mir-	1320	cgaccgcacccgcccgaagcUgggUcaaggagCCCAGCAGGACG
  			4530		GGAGCGcggcgc
  1027	AUGGAGAAGGCUUCUGA	hsa-mir-2437	hsa-mir-	1321	gccUaggagUccUUggUcagUggggacAUGGAGAAGGCUUC
  			4531		UGAgga
  1028	AAAAGCUGGGUUGAGAA	hsa-mir-2438	hsa-mir-	1322	gccUUcUcUUcccagUUcUUccUggagUcggggAAAAGCUG
  			320e		GGUUGAGAAggUgaaaaga
  1029	UGGAAGGUAGACGGCCAGAGAG	hsa-mir-2439	hsa-mir-	1323	ggUcaccUgUcUggccagcUacgUccccacggcccUUgUcagUg
  			3190		UGGAAGGUAGACGGCCAGAGAGgUgaccc
  1030	UCUGGGAGGUUGUAGCAGUGGA	hsa-mir-2440	hsa-mir-	1324	aggaagggaUUCUGGGAGGUUGUAGCAGUGGAaaaagUU
  			3192		cUUUUcUUccUcUgaUcgcccUcUcagcUcUUUccUUcUg
  1031	CCCCGGGGAGCCCGGCG	hsa-mir-2441	hsa-mir-	1325	acagaCCCCGGGGAGCCCGGCGgUgaagcUccUggUaUccU
  			4532		gggUgUcUga
  1032	UGGAAGGAGGUUGCCGGACGCU	hsa-mir-2442	hsa-mir-	1326	UgagaaUgUGGAAGGAGGUUGCCGGACGCUgcUggcUgc
  			4533		cUUccagcgUccacUUcccUUUcUcUcUcUcc
  1033	CAAGAACCUCAAUUACCUUUGC	hsa-mir-2443	pending	1327	cggUUggUgcaaaggUaaUUgUggUUUcUgccaUUgaaagUa
  					aaggCAAGAACCUCAAUUACCUUUGCagcgaccU
  1034	AAUCUGAGAAGGCGCACAAGGU	hsa-mir-2444	has-mir-	1328	UcgagggAAUCUGAGAAGGCGCACAAGGUUUgUgUccaa
  			3200		UacagUccacaccUUgcgcUacUcaggUcUgcUcgUg
  1035	CACCUUGCGCUACUCAGGUCUG	hsa-mir-2444*	hsa-mir-	1329	UcgagggaaUcUgagaaggcgcacaaggUUUgUgUccaaUacag
  			3200		UccaCACCUUGCGCUACUCAGGUCUGcUcgU
  1036	GGAGGAACCUUGGAGCUUCGGC	hsa-mir-2445	hsa-mir-	1330	ggcUgaagcUcUaaggUUccgccUgcgggcaggaagcGGAGGA
  			3928		ACCUUGGAGCUUCGGCa
  1037	GGAUGGAGGAGGGGUCU	hsa-mir-2446	hsa-mir-	1331	UgUgaaUgacccccUUccagagccaaaaUcaccagGGAUGGAG
  			4534		GAGGGGUCUUgggUacU
  1038	ACUGGACUAGGAGUCAGAAGG	hsa-mir-2447	hsa-mir-	1332	gggagcACUGGACUAGGAGUCAGAAGGUggagUUcUggg
  			378i		UgcUgUUUUcccacUcUUgggcccUgggcaUgUUcUg
  1039	GUGGACCUGGCUGGGAC	hsa-mir-2448	hsa-mir-	1333	aacUgggUcccagUcUUcacagUUggUUUcUgacacGUGGAC
  			4535		CUGGCUGGGACgaUgUg
  1040	ACCUGGACCCAGCGUAGACAAA	hsa-mir-2449	hsa-mir-	1334	ccACCUGGACCCAGCGUAGACAAAgaggUgUUUcUacUcc
  			3690		aUaUcUaccUggacccagUgU
  1041	CAAAAACUGCAGUUACUUUUGU	hsa-mir-2450	hsa-mir-	1335	agUUggUgcaaaagUaaUUgcggUUUUUgccgUcgaaaaUaa
  			548am		UggCAAAAACUGCAGUUACUUUUGUaccaaUg
  1042	UUGGGCUGGGCUGGGUUGGG	hsa-mir-2451	hsa-mir-	1336	UUUGGGCUGGGCUGGGUUGGGcagUUcUUcUgcUggac
  			1587		UcaccUgUgaccagc
  1043	UGUGGUAGAUAUAUGCACGAU	hsa-mir-2452	hsa-mir-	1337	aUgUgUggUagaUaUaUgcacUgUaUaUaaacaUaaUGUGG
  			4536		UAGAUAUAUGCACGAUaUag
  1044	AGAAGGGAAAGAACAUCAA	hsa-mir-2453	pending	1338	aUggUgUUUgccUccUUcaUccgcaaggcaUcUgaUgcccacga
  					agUUaggaaggUccUUggggAGAAGGGAAAGAACAUCAAa
  1045	CUGUCCUAAGGUUGUUGAGUU	hsa-mir-2454	hsa-mir-	1339	cgcaUgacUcUUcaaccUcaggacUUgcagaaUUaaUggaaUg
  			676		CUGUCCUAAGGUUGUUGAGUUgUgca
  1046	AAAAGGCAUUGUGGUUUUUG	hsa-mir-2455	hsa-mir-	1340	caUUaggUUggUgcAAAAGGCAUUGUGGUUUUUGccUa
  			548an		UaaaagUaaUggcaaaaaccgcaaUUccUUUUgcaccaaccUaa
  					U
  1047	CGGGCGGCGGCUGUGUUGCGCA	hsa-mir-2456	pending	1341	cacUcgcgcUgcggccagcgcccgggccUgcgggccCGGGCGGCG
  					GCUGUGUUGCGCAgUc
  1048	UAUGGAAGGGAGAAGAGCUUUA	hsa-mir-2457	hsa-mir-	1342	UUaUUaaUAUGGAAGGGAGAAGAGCUUUAaUgaUUgga
  			3202-1		gUcaUUUUcagagcaUUaaagcUcUUcUcccUUccaUaUUaa
  					UgU
  1049	CGGCGGGGACGGCGAUUGGU	hsa-mir-1908	hsa-mir-	1343	UgccgCGGCGGGGACGGCGAUUGGUccgUaUgUgUggUg
  			1908		ccaccggccgccggcUccgccccggcc
  1050	CCUCCUGCCCUCCUUGCUGUAG	hsa-mir-1976	pending	1344	gcaaagggUggcagcaaggaaggcaggggUccUaaggUgUgUCC
  					UCCUGCCCUCCUUGCUGUAGacUUUgg
  1051	UGAGCCGAGCUGAGCUUAGCUG	hsa-mir-2457	hsa-mir-	1345	UGAGCCGAGCUGAGCUUAGCUGggcUgagcUa
  			4537		accagggcUgggcUgagcUgggcUgagcUgagcUgagc
  					GAGCUUGGAUGAGCUGGGCUGAacUgggcUgg
  1052	GAGCUUGGAUGAGCUGGGCUGA	hsa-mir-2458	hsa-mir-	1346	gUUgagcUgggcUgggcUgagUUgagccaggcUgaU
  			4538		cUgggcUgag
  1053	GCUGAACUGGGCUGAGCUGGGC	hsa-mir-2459	hsa-mir-	1347	UgagcUgggcUcUgcUgUgcUgUgcUgagcagggcU
  			4539		gaGCUGAACUGGGCUGAGCUGGGC
  1054	CUGGGCUGAAUGACAGUGAUGAG	hsa-mir-2460	pending	1348	gUcaagUcagaacagccaggUagagcccUUgUccaaac
  					CUGGGCUGAAUGACAGUGAUGAG
  1055	GGGAGCCGGGGCUGUGAGAGGA	hsa-mir-2461	pending	1349	cUcUUUgagccUUggcUgccUUggUgcagcagggUc
  					aUcUgUagggccaccccacagcUcUUUccUUccccUc
  					cUcUcUccaGGGAGCCGGGGCUGUGAGAGGA
  1056	UUAGUCCUGCCUGUAGGUUUA	hsa-mir-2462	hsa-mir-	1350	aagcUgcaUggaccaggacUUggcaccUUUggccUU
  			4540		AGUCCUGCCUGUAGGUUUA

• TABLE 33
  Primers for Reverse Transcription for a subset of the known miRNAs
  identified by deep sequencing analysis.

  SEQ	Putative		Primer for reverse	SEQ
  ID	Major/Minor	Mature Sequence	transcription	ID
  NO.	miRNA ID	Captured	of miRNA	NO.
  7	hsa-mir-129-	AAGCCCUUACCCCAAAAAGCAU	AAGCCCTTACCCCAAAAAGCAT	1351
  	2*
  22	hsa-mir-25*	AGGCGGAGACUUGGGCAAUUG	AGGCGGAGACTTGGGCAATTG	1352
  24	hsa-mir-30b*	CUGGGAGGUGGAUGUUUACUUC	CTGGGAGGTGGATGTTTACTTC	1353
  26	hsa-mir-30c-	CUGGGAGAAGGCUGUUUACUCU	CTGGGAGAAGGCTGTTTACTCT	1354
  	2*
  27	hsa-mir-30e*	CUUUCAGUCGGAUGUUUACAGC	CTTTCAGTCGGATGTTTACAGC	1355
  29	hsa-mir-342-	UCUCACACAGAAAUCGCACCCGU	TCTCACACAGAAATCGCACCCGT	1356
  	3p
  56	hsa-let-7a-2	UGAGGUAGUAGGUUGUAUAGUU	TGAGGTAGTAGGTTGTATAGTT	1357
  57	hsa-let-7a-3	UGAGGUAGUAGGUUGUAUAGUU	TGAGGTAGTAGGTTGTATAGTT	1358
  58	hsa-let-7c	UGAGGUAGUAGGUUGUAUGGUU	TGAGGTAGTAGGTTGTATGGTT	1359
  63	hsa-mir-100	AACCCGUAGAUCCGAACUUGUG	AACCCGTAGATCCGAACTTGTG	1360
  64	hsa-mir-101-1	UACAGUACUGUGAUAACUGAA	TACAGTACTGTGATAACTGAA	1361
  65	hsa-mir-101-2	GUACAGUACUGUGAUAACUGAA	TACAGTACTGTGATAACTGAA	1362
  66	hsa-mir-103-1	AGCAGCAUUGUACAGGGCUAUGA	AGCAGCATTGTACAGGGCTATGA	1363
  67	hsa-mir-103-2	AGCAGCAUUGUACAGGGCUAUGA	AGCAGCATTGTACAGGGCTATGA	1364
  70	hsa-mir-106a	AAAAGUGCUUACAGUGCAGGUAG	AAAAGTGCTTACAGTGCAGGTAG	1365
  71	hsa-mir-106b	UAAAGUGCUGACAGUGCAGAU	TAAAGTGCTGACAGTGCAGAT	1366
  72	hsa-mir-107	AGCAGCAUUGUACAGGGCUAUCA	AGCAGCATTGTACAGGGCTATCA	1367
  73	hsa-mir-10a	UACCCUGUAGAUCCGAAUUUGUG	TACCCTGTAGATCCGAATTTGTG	1368
  74	hsa-mir-10b	UACCCUGUAGAACCGAAUUUGUG	TACCCTGTAGAACCGAATTTGTG	1369
  98	hsa-mir-128-1	UCACAGUGAACCGGUCUCUUU	TCACAGTGAACCGGTCTCTTT	1370
  99	hsa-mir-128-2	UCACAGUGAACCGGUCUCUUU	TCACAGTGAACCGGTCTCTTT	1371
  100	hsa-mir-129-1	CUUUUUGCGGUCUGGGCUUGC	CTTTTTGCGGTCTGGGCTTGC	1372
  101	hsa-mir-129-2	CUUUUUGCGGUCUGGGCUUGC	CTTTTTGCGGTCTGGGCTTGC	1373
  103	hsa-mir-1295	UUAGGCCGCAGAUCUGGGUGA	TTAGGCCGCAGATCTGGGTGA	1374
  119	hsa-mir-140-	UACCACAGGGUAGAACCACGG	TACCACAGGGTAGAACCACGG	1375
  	3p
  122	hsa-mir-143	UGAGAUGAAGCACUGUAGCUC	TGAGATGAAGCACTGTAGCTC	1376
  125	hsa-mir-146a	UGAGAACUGAAUUCCAUGGGUU	TGAGAACTGAATTCCATGGGTT	1377
  126	hsa-mir-146b	UGAGAACUGAAUUCCAUAGGCU	TGAGAACTGAATTCCATAGGCT	1378
  127	hsa-mir-148a	UCAGUGCACUACAGAACUUUGU	TCAGTGCACTACAGAACTTTGT	1379
  130	hsa-mir-151-	CUAGACUGAAGCUCCUUGAGG	CTAGACTGAAGCTCCTTGAGG	1380
  	3p
  131	hsa-mir-152	UCAGUGCAUGACAGAACUUGG	TCAGTGCATGACAGAACTTGG	1381
  132	hsa-mir-155	UUAAUGCUAAUCGUGAUAGGGGU	TTAATGCTAATCGTGATAGGGGT	1382
  133	hsa-mir-15a	UAGCAGCACAUAAUGGUUUGUG	TAGCAGCACATAATGGTTTGTG	1383
  134	hsa-mir-15b	UAGCAGCACAUCAUGGUUUACA	TAGCAGCACATCATGGTTTACA	1384
  135	hsa-mir-16-1	UAGCAGCACGUAAAUAUUGGCG	TAGCAGCACGTAAATATTGGCG	1385
  136	hsa-mir-16-2	UAGCAGCACGUAAAUAUUGGCG	TAGCAGCACGTAAATATTGGCG	1386
  137	hsa-mir-17	CAAAGUGCUUACAGUGCAGGUAG	CAAAGTGCTTACAGTGCAGGTAG	1387
  138	hsa-mir-181a-	AACAUUCAACGCUGUCGGUGAGU	AACATTCAACGCTGTCGGTGAGT	1388
  	1
  139	hsa-mir-181a-	AACAUUCAACGCUGUCGGUGAGU	AACATTCAACGCTGTCGGTGAGT	1389
  	2
  140	hsa-mir-181b-	AACAUUCAUUGCUGUCGGUGGGU	AACATTCATTGCTGTCGGTGGG	1390
  	1
  141	hsa-mir-181b-	AACAUUCAUUGCUGUCGGUGGGU	AACATTCATTGCTGTCGGTGGG	1391
  	2
  142	hsa-mir-181c	AACAUUCAACCUGUCGGUGAGU	AACATTCAACCTGTCGGTGAGT	1392
  143	hsa-mir-181d	AACAUUCAUUGUUGUCGGUGGGU	AACATTCATTGTTGTCGGTGGGTT	1393
  147	hsa-mir-185	UGGAGAGAAAGGCAGUUCCUGA	TGGAGAGAAAGGCAGTTCCTGA	1394
  150	hsa-mir-18a	UAAGGUGCAUCUAGUGCAGAUAG	TAAGGTGCATCTAGTGCAGATAG	1395
  151	hsa-mir-191	CAACGGAAUCCCAAAAGCAGCUG	CAACGGAATCCCAAAAGCAGCTG	1396
  152	hsa-mir-192	CUGACCUAUGAAUUGACAGCC	CTGACCTATGAATTGACAGCC	1397
  153	hsa-mir-193a-	UGGGUCUUUGCGGGCGAGAUGA	TGGGTCTTTGCGGGCGAGATGA	1398
  	5p
  159	hsa-mir-196b	UAGGUAGUUUCCUGUUGUUGGG	TAGGTAGTTTCCTGTTGTTGGG	1399
  160	hsa-mir-197	UUCACCACCUUCUCCACCCAGC	TTCACCACCTTCTCCACCCAGC	1400
  163	hsa-mir-199b	ACAGUAGUCUGCACAUUGGUUA	ACAGTAGTCTGCACATTGGTTA	1401
  164	hsa-mir-19a	UGUGCAAAUCUAUGCAAAACUGA	TGTGCAAATCTATGCAAAACTGA	1402
  175	hsa-mir-20a	UAAAGUGCUUAUAGUGCAGGUAG	TAAAGTGCTTATAGTGCAGGTAG	1403
  176	hsa-mir-20b	CAAAGUGCUCAUAGUGCAGGUAG	CAAAGTGCTCATAGTGCAGGTAG	1404
  186	hsa-mir-221	AGCUACAUUGUCUGCUGGGUUUC	AGCTACATTGTCTGCTGGGTTTC	1405
  187	hsa-mir-222	AGCUACAUCUGGCUACUGGGU	AGCTACATCTGGCTACTGGGT	1406
  190	hsa-mir-23a	AUCACAUUGCCAGGGAUUUCC	ATCACATTGCCAGGGATTTCC	1407
  194	hsa-mir-25	CAUUGCACUUGUCUCGGUCUGA	CATTGCACTTGTCTCGGTCTGA	1408
  195	hsa-mir-26a-1	UUCAAGUAAUCCAGGAUAGGCU	TTCAAGTAATCCAGGATAGGCT	1409
  196	hsa-mir-26a-2	UUCAAGUAAUCCAGGAUAGGCU	TTCAAGTAATCCAGGATAGGCT	1410
  197	hsa-mir-26b	UUCAAGUAAUUCAGGAUAGGU	TTCAAGTAATTCAGGATAGGT	1411
  198	hsa-mir-27a	UUCACAGUGGCUAAGUUCCGC	TTCACAGTGGCTAAGTTCCGC	1412
  203	hsa-mir-29a	UAGCACCAUCUGAAAUCGGUUA	TAGCACCATCTGAAATCGGTTA	1413
  208	hsa-mir-30a	UGUAAACAUCCUCGACUGGAAG	TGTAAACATCCTCGACTGGAAG	1414
  209	hsa-mir-30b	UGUAAACAUCCUACACUCAGCU	TGTAAACATCCTACACTCAGCT	1415
  210	hsa-mir-30c-1	UGUAAACAUCCUACACUCUCAGC	TGTAAACATCCTACACTCTCAGC	1416
  211	hsa-mir-30c-2	UGUAAACAUCCUACACUCUCAGC	TGTAAACATCCTACACTCTCAGC	1417
  212	hsa-mir-30d	UGUAAACAUCCCCGACUGGAAG	TGTAAACATCCCCGACTGGAAG	1418
  213	hsa-mir-30e	UGUAAACAUCCUUGACUGGAAG	TGTAAACATCCTTGACTGGAAG	1419
  216	hsa-mir-320a	AAAAGCUGGGUUGAGAGGGCGA	AAAAGCTGGGTTGAGAGGGCGA	1420
  217	hsa-mir-320b-	AAAAGCUGGGUUGAGAGGGCAA	AAAAGCTGGGTTGAGAGGGCAA	1421
  	1
  218	hsa-mir-320b-	AAAAGCUGGGUUGAGAGGGCAA	AAAAGCTGGGTTGAGAGGGCAA	1422
  	2
  227	hsa-mir-331	GCCCCUGGGCCUAUCCUAGAA	GCCCCTGGGCCTATCCTAGAA	1423
  231	hsa-mir-33a	GUGCAUUGUAGUUGCAUUGCA	GTGCATTGTAGTTGCATTGCA	1424
  233	hsa-mir-340	UUAUAAAGCAAUGAGACUGAUU	TTATAAAGCAATGAGACTGATT	1425
  236	hsa-mir-34a	UGGCAGUGUCUUAGCUGGUUGU	TGGCAGTGTCTTAGCTGGTTGT	1426
  239	hsa-mir-361-	UUAUCAGAAUCUCCAGGGGUAC	TTATCAGAATCTCCAGGGGTAC	1427
  	5p
  241	hsa-mir-363	AAUUGCACGGUAUCCAUCUGUA	AATTGCACGGTATCCATCTGTA	1428
  242	hsa-mir-365-2	UAAUGCCCCUAAAAAUCCUUAU	TAATGCCCCTAAAAATCCTTAT	1429
  246	hsa-mir-374a	UUAUAAUACAACCUGAUAAGUG	TTATAATACAACCTGATAAGTG	1430
  250	hsa-mir-378	ACUGGACUUGGAGUCAGAAGG	ACTGGACTTGGAGTCAGAAGG	1431
  259	hsa-mir-423	UGAGGGGCAGAGAGCGAGACUUU	TGAGGGGCAGAGAGCGAGACTTT	1432
  265	hsa-mir-449a	UGGCAGUGUAUUGUUAGCUGGU	TGGCAGTGTATTGTTAGCTGGT	1433
  287	hsa-mir-503	UAGCAGCGGGAACAGUUCUGCAG	TAGCAGCGGGAACAGTTCTGCAG	1434
  296	hsa-mir-532	CAUGCCUUGAGUGUAGGACCGU	CATGCCTTGAGTGTAGGACCGT	1435
  316	hsa-mir-576-	AAGAUGUGGAAAAAUUGGAAUC	AAGATGTGGAAAAATTGGAATC	1436
  	3p
  327	hsa-mir-625	AGGGGGAAAGUUCUAUAGUCC	AGGGGGAAAGTTCTATAGTCC	1437
  338	hsa-mir-7-1	UGGAAGACUAGUGAUUUUGUUGU	TGGAAGACTAGTGATTTTGTTGT	1438
  339	hsa-mir-7-2	UGGAAGACUAGUGAUUUUGUUGU	TGGAAGACTAGTGATTTTGTTGT	1439
  340	hsa-mir-7-3	UGGAAGACUAGUGAUUUUGUUGU	TGGAAGACTAGTGATTTTGTTGT	1440
  345	hsa-mir-874	CUGCCCUGGCCCGAGGGACCGA	CTGCCCTGGCCCGAGGGACCGA	1441
  351	hsa-mir-9-1	UCUUUGGUUAUCUAGCUGUAUGA	TCTTTGGTTATCTAGCTGTATGA	1442
  352	hsa-mir-9-2	UCUUUGGUUAUCUAGCUGUAUGA	TCTTTGGTTATCTAGCTGTATGA	1443
  353	hsa-mir-92a-1	UAUUGCACUUGUCCCGGCCUGU	TATTGCACTTGTCCCGGCCTGT	1444
  354	hsa-mir-92a-2	UAUUGCACUUGUCCCGGCCUGU	TATTGCACTTGTCCCGGCCTGT	1445
  356	hsa-mir-93	CAAAGUGCUGUUCGUGCAGGUAG	CAAAGTGCTGTTCGTGCAGGTAG	1446
  357	hsa-mir-9-3	UCUUUGGUUAUCUAGCUGUAUGA	TCTTTGGTTATCTAGCTGTATGA	1447
  364	hsa-mir-99a	AACCCGUAGAUCCGAUCUUGUG	AACCCGTAGATCCGATCTTGTG	1448
  387	hsa-mir-570	AAAGGUAAUUGCAGUUUUUCCC	AAAGGTAATTGCAGTTTTTCCCA	1449

• TABLE 34
  Primers for Reverse Transcription of a subset of novel miRNAs
  identified by deep sequencing analysis.

  SEQ	Temporary		Primer for Reverse	SEQ
  ID	Assigned	Mature Sequence	Transcription	ID
  NO.	miRNA ID	Captured	of miRNA	NO.
  773	hsa-miR-	ACUGGACUUGGAGUCAGGA	ACTGGACTTGGAGTCAGGA	1450
  	378c
  774	hsa-mir-449c	AGGCAGUGUAUUGCUAGCGGCU	AGGCAGTGTATTGCTAGCGGCTGT	1451
  775	hsa-mir-500-	UGCACCCAGGCAAGGAUUCUGC	TGCACCCAGGCAAGGATTCTGC	1452
  	2
  784	hsa-mir-2224	UGAGGGAGGAGACUGCA	TGAGGGAGGAGACTGCA	1453
  785	hsa-mir-2225	ACUGGACUUGGAGCCAGAAG	ACTGGACTTGGAGCCAGAAG	1454
  786	hsa-mir-2226	GUCACUGAUGUCUGUAGCUGAG	GTCACTGATGTCTGTAGCTGAGACGG	1455
  787	hsa-mir-2227	GAUGAGGAUGGAUAGCAAGGAA	GATGAGGATGGATAGCAAGGAAG	1456
  789	hsa-mir-2229	AAAAGCAUCAGGAAGUACCCA	AAAAGCATCAGGAAGTACCCA	1457
  796	hsa-mir-2235	GUCAAAUGAAGGGCUGAUCACG	GTCAAATGAAGGGCTGATCACGAAATA	1458
  798	hsa-mir-2237	AGAGUUAACUCAAAAUGGACUA	AGAGTTAACTCAAAATGGACTA		1459
  799	hsa-mir-2238	UGUUGGGAUUCAGCAGGACCAU	TGTTGGGATTCAGCAGGACCATT	1460
  800	hsa-mir-2239	UAAAUAGAGUAGGCAAAGGACA	TAAATAGAGTAGGCAAAGGACA	1461
  805	hsa-mir-2244	AAGAGGAAGAAAUGGCUGGUUC	AAGAGGAAGAAATGGCTGGTTCTCAG	1462
  809	hsa-mir-2248	UAGUGGAUGAUGCACUCUGUGC	TAGTGGATGATGCACTCTGTGC	1463
  814	hsa-mir-2253	AAAGACUCUGCAAGAUGCCU	AAAGACTCTGCAAGATGCCT	1464
  816	hsa-mir-2255	AGGAGAAGUAAAGUAGAA	AGGAGAAGTAAAGTAGAA	1465
  817	hsa-mir-2256	AUGGCCAGAGCUCACACAGAGG	ATGGCCAGAGCTCACACAGAGG	1466
  819	hsa-mir-2258	AUCAGGGCUUGUGGAAUGGGAA	ATCAGGGCTTGTGGAATGGGAAG	1467
  820	hsa-mir-2259	AUGGCCAGAGCUCACACAGAGG	ATGGCCAGAGCTCACACAGAGG	1468
  821	hsa-mir-2260	UGAGGAUAUGGCAGGGAAGGGG	TGAGGATATGGCAGGGAAGGGGA	1469
  825	hsa-mir-2264	UGGGCUCAGGGUACAAAGGUU	TGGGCTCAGGGTACAAAGGTTC	1470
  828	hsa-mir-	GCUGCACCGGAGACUGGGUAA	GCTGCACCGGAGACTGGGTAA	1471
  	2266-1
  829	hsa-mir-	GCUGCACCGGAGACUGGGUAA	GCTGCACCGGAGACTGGGTAA	1472
  	2266-2
  836	hsa-mir-2272	UGUCGUGGGGCUUGCUGGCUUG	TGTCGTGGGGCTTGCTGGCTTG	1473
  838	hsa-mir-2274	ACUGGACUUGGAGGCAGAA	ACTGGACTTGGAGGCAGAA	1474
  841	hsa-mir-2277	UUGGAGGCGUGGGUUUU	TTGGAGGCGTGGGTTTT	1475
  844	hsa-mir-2280	CUGACUGAAUAGGUAGGGUCAU	CTGACTGAATAGGTAGGGTCAT	1476
  846	hsa-mir-2282	AGAUUGUUUCUUUUGCCGUGCA	AGATTGTTTCTTTTGCCGTGCA	1477
  847	hsa-mir-	CACGGCAAAAGAAACAAUCCA	CACGGCAAAAGAAACAATCCA	1478
  	2282*
  848	hsa-mir-2283	CAGGGCUGGCAGUGACAUGGGU	CAGGGCTGGCAGTGACATGGGT	1479
  849	hsa-mir-2284	GGUGGGGGCUGUUGUUU	GGTGGGGGCTGTTGTTT	1480
  850	hsa-mir-2285	UGGGGAGGUGUGGAGUCAGCAU	TGGGGAGGTGTGGAGTCAGCATG	1481
  852	hsa-mir-2287	GGCUCCUUGGUCUAGGGGUA	GGCTCCTTGGTCTAGGGGTA	1482
  856	hsa-mir-2290	UGGGGAUUUGGAGAAGUGGUGA	TGGGGATTTGGAGAAGTGGTGA	1483
  857	hsa-mir-2291	AAAAGUGAUUGCAGUGUUUG	AAAAGTGATTGCAGTGTTTGCC	1484
  860	hsa-mir-2294	UAGGAGCUCAACAGAUGCCUGU	TAGGAGCTCAACAGATGCCTGT	1485
  861	hsa-mir-2295	AGCUUUUGGGAAUUCAGGUAG	AGCTTTTGGGAATTCAGGTAG	1486
  864	hsa-mir-2298	CAAAAGUGAUCGUGGUUUUUG	CAAAAGTGATCGTGGTTTTTG	1487
  865	hsa-mir-2299	AGGGUGUGUGUGUUUUU	AGGGTGTGTGTGTTTTT	1488
  871	hsa-mir-2305	ACUGACAGGAGAGCAUUUUGA	ACTGACAGGAGAGCATTTTGA	1489
  874	hsa-mir-2308	AUAGUGGUUGUGAAUUUACCUU	ATAGTGGTTGTGAATTTACCTTC	1490
  876	hsa-mir-2310	CUACCCCAGGAUGCCAGCAUAG	CTACCCCAGGATGCCAGCATAGTT	1491
  877	hsa-mir-2311	ACUGGACUUGGUGUCAGAUGG	ACTGGACTTGGTGTCAGATGG	1492
  880	hsa-mir-2314	UAGUGGAUGAUGGAGACUCGGU	TAGTGGATGATGGAGACTCGGT	1493
  888	hsa-mir-2322	AAGGUUUGGAUAGAUGCAAUA	AAGGTTTGGATAGATGCAATA	1494
  889	hsa-mir-2323	AAAGGUAAUUGCAGUUUUUCCC	AAAGGTAATTGCAGTTTTTCCCA	1495
  890	hsa-mir-2324	AGGGGACCAAAGAGAUAUAUAG	AGGGGACCAAAGAGATATATAG	1496
  896	hsa-mir-2328	GGCGACAAAACGAGACCCUGU	GGCGACAAAACGAGACCCTGTC	1497
  897	hsa-mir-2329	GGGUGCGGGCCGGCGGGG	GGGTGCGGGCCGGCGGGGT	1498
  899	hsa-mir-2331	CAUGCUAGGAUAGAAAGAAUGG	CATGCTAGGATAGAAAGAATGG	1499
  901	hsa-mir-2333	GCAAAGUGAUGAGUAAUACUGG	GCAAAGTGATGAGTAATACTGG	1500
  915	hsa-mir-2346	CCCUGGGGUUCUGAGGACAUG	CCCTGGGGTTCTGAGGACATG	1501
  917	hsa-mir-2348	CAGGAAGGAUUUAGGGACAGGC	CAGGAAGGATTTAGGGACAGGC	1502
  919	hsa-mir-2350	AUUAAGGACAUUUGUGAUUGAU	ATTAAGGACATTTGTGATTGAT	1503
  920	hsa-mir-	AAAAGGCAUAAAACCAAGACA	AAAAGGCATAAAACCAAGACA	1504
  	2351-1
  921	hsa-mir-	AAAAGGCAUAAAACCAAGACA	AAAAGGCATAAAACCAAGACA	1505
  	2351-2
  924	hsa-mir-	UAAAAACUGCAAUUACUUUC	TAAAAACTGCAATTACTTTC	1506
  	2354-1
  926	hsa-mir-	UGUGAUAUCAUGGUUCCUGGGA	TGTGATATCATGGTTCCTGGGA	1507
  	2355-1
  929	hsa-mir-	UGUGAUAUCAUGGUUCCUGGGA	TGTGATATCATGGTTCCTGGGA	1508
  	2355-2
  931	hsa-mir-	UGUGAUAUCAUGGUUCCUGGGA	TGTGATATCATGGTTCCTGGGA	1509
  	2355-3
  932	hsa-mir-	UGUGAUAUCGUGCUUCCUGGGA	TGTGATATCGTGCTTCCTGGGA	1510
  	2355b
  936	hsa-mir-2358	AAAAGUAACUGCGGUUUUUGA	AAAAGTAACTGCGGTTTTTGA	1511
  938	hsa-mir-2360	GGAGUGGGCUGGUGGUU	GGAGTGGGCTGGTGGTT	1512
  940	hsa-mir-	AAGGGCUUCCUCUCUGCAGGAC	AAGGGCTTCCTCTCTGCAGGAC	1513
  	2362-1
  941	hsa-mir-	AAGGGCUUCCUCUCUGCAGGAC	AAGGGCTTCCTCTCTGCAGGAC	1514
  	2362-2
  949	hsa-mir-2370	AGAGCUGGCUGAAGGGCAG	AGAGCTGGCTGAAGGGCAG	1515
  953	hsa-mir-2374	UGUGACUUUAAGGGAAAUGGCG	TGTGACTTTAAGGGAAATGGCG	1516
  954	hsa-mir-2375	UCUCAGGAGUAAAGACAGAGUU	TCTCAGGAGTAAAGACAGAGTT	1517
  955	hsa-mir-2376	AGGUGGAUGCAAUGUGACCUCA	AGGTGGATGCAATGTGACCTCA	1518
  977	hsa-mir-2397	AGGCUGGGCUGGGACGGA	AGGCTGGGCTGGGACGGA	1519
  979	hsa-mir-2399	UAGGAUGGGGGUGAGAGGUG	TAGGATGGGGGTGAGAGGTG	1520
  988	hsa-mir-2406	UGAGGGAGUAGGAUGUAUGGUU	TGAGGGAGTAGGATGTATGGTT	1521
  991	hsa-mir-2409	AGACUGACGGCUGGAGGCCCAU	AGACTGACGGCTGGAGGCCCAT	1522
  994	hsa-mir-2412	UAGUGAGUUAGAGAUGCAGAGC	TAGTGAGTTAGAGATGCAGAGC	1523
  996	hsa-mir-2414	GGGAGAAGGGUCGGGGC	GGGAGAAGGGTCGGGGC	1524
  1002	hsa-mir-2416	CUCGUGGGCUCUGGCCACGGC	CTCGTGGGCTCTGGCCACGGCC	1525
  1003	hsa-mir-	AGAAGGGGUGAAAUUUAAACGU	AGAAGGGGTGAAATTTAAACGT	1526
  	2417-1
  1004	hsa-mir-	AGAAGGGGUGAAAUUUAAACGU	AGAAGGGGTGAAATTTAAACGT	1527
  	2417-2
  1005	hsa-mir-	AGAAGGGGUGAAAUUUAAACGU	AGAAGGGGTGAAATTTAAACGT	1528
  	2417-3
  1007	hsa-mir-2419	GCUCAGGGAUGAUAACUGUGCUGAGA	GCTCAGGGATGATAACTGTGCTGAGA	1529
  1011	hsa-mir-2423	CUGGACUGAGCCAUGCUACUGG	CTGGACTGAGCCATGCTACTGG	1530
  1017	hsa-mir-2428	AUAGCAGCAUGAACCUGUCUCA	ATAGCAGCATGAACCTGTCTCA	1531
  1018	hsa-mir-	UGAGACAGGCUUAUGCUGCUAU	TGAGACAGGCTTATGCTGCTAT	1532
  	2428*
  1022	hsa-mir-2432	UGGUCUGCAAAGAGAUGACUGU	TGGTCTGCAAAGAGATGACTGTG	1533
  1025	hsa-mir-2435	UUGGAGGGUGUGGAAGACAUC	TTGGAGGGTGTGGAAGACATC	1534
  1027	hsa-mir-2437	AUGGAGAAGGCUUCUGA	ATGGAGAAGGCTTCTGA	1535
  1028	hsa-mir-2438	AAAAGCUGGGUUGAGAA	AAAAGCTGGGTTGAGAAG	1536
  1029	hsa-mir-2439	UGGAAGGUAGACGGCCAGAGAG	TGGAAGGTAGACGGCCAGAGAG	1537
  1030	hsa-mir-2440	UCUGGGAGGUUGUAGCAGUGGA	TCTGGGAGGTTGTAGCAGTGGA	1538
  1036	hsa-mir-2445	GGAGGAACCUUGGAGCUUCGGC	GGAGGAACCTTGGAGCTTCGGCA	1539
  1038	hsa-mir-2447	ACUGGACUAGGAGUCAGAAGG	ACTGGACTAGGAGTCAGAAGG	1540
  1041	hsa-mir-2450	CAAAAACUGCAGUUACUUUUGU	CAAAAACTGCAGTTACTTTTGT	1541
  1046	hsa-mir-2455	AAAAGGCAUUGUGGUUUUUG	AAAAGGCATTGTGGTTTTTG	1542

• TABLE 35
  miRNAs that differentiate ABC DLBCL vs.
  GCB DLBCL

  	FoldChange	pVal
  	ABC vs.	ABC vs.
  MicroRNA	GCB	GCB

  UGGUCUGCAAAGAGAUGACUGUG	−4.30138	0.001335
  (SEQ ID NO. 1565)
  CAAAAACUGCAGUUACUUUUGU	−3.52085	0.022949
  (SEQ ID NO. 1041)
  UGGGGAUUUGGAGAAGUGGUGA	−2.8374	0.000753
  (SEQ ID NO. 856)
  hsa-miR-129-3p	−2.48708	7.39E−05
  ACUGGACUUGGUGUCAGAUGG	−1.3712	0.01173
  (SEQ ID NO. 877)
  hsa-miR-196b	−1.28463	0.032606
  hsa-miR-9	−1.00737	0.003176
  hsa-miR-28-5p	−0.82319	0.00026
  hsa-miR-365	−0.78573	0.032749
  hsa-miR-185	−0.75345	0.044082
  hsa-miR-199b-3p	−0.74898	0.015518
  hsa-miR-152	−0.67388	0.001282
  hsa-miR-23a	−0.65979	0.028732
  hsa-miR-193a-5p	−0.54233	0.031154
  hsa-miR-27a	−0.49729	0.027207
  hsa-miR-331-3p	−0.47573	0.0128
  hsa-miR-301a	−0.45261	0.014231
  hsa-miR-128	−0.4224	0.024065
  AGAUUGUUUCUUUUGCCGUGCA	0.557435	0.02141
  (SEQ ID NO. 846)
  hsa-miR-625	0.747299	0.01813
  hsa-miR-155	0.781901	0.024175
  hsa-miR-20b	0.986918	0.000746
  GGCUCCUUGGUCUAGGGGUA	1.945372	0.002596
  (SEQ ID NO. 852)
  UAGUGAGUUAGAGAUGCAGAGC	2.347695	0.009081
  (SEQ ID NO. 994)
  CAGGAAGGAUUUAGGGACAGGC	2.519326	0.04483
  (SEQ ID NO.-917)
  *The 3′-end G of SEQ ID NO. 1565 is optionally omitted (providing SEQ ID NO. 1022).

• APPENDIX A
  Average expression of the genes depicted in FIG. 6*

  	Naïve
  	vs	Germinal	Germinal
  	Germinal	Center vs	Center vs
  	Center	Plasma	Memory

  		Naïve	GC	GC	PC	GC
  		Aver-	Aver-	aver-	Aver-	Aver-	Memory
  ID	Gene Name	age	age	age	age	age	Average

  1	VPS37B	10.60	4.58	4.58	9.71
  2	SCN3A	9.50	3.98
  3	NT5E	10.76	5.69
  4	TBC1D9	12.03	7.03	7.03	11.19	7.03	12.88
  5	MAP7					1.64	8.03
  6	SPRY1	10.40	5.72
  7	TNFSF12	8.11	3.54			3.54	7.55
  8	SOCS3	9.00	4.48	4.48	9.39
  9	EDG1	11.43	6.91			6.91	8.69
  10	CTGF	7.62	3.30	3.30	9.65
  11	FAM46A	10.65	6.46			6.46	8.29
  12	MOBKL2B	11.63	7.45
  13	DNMT3A	10.56	6.44	6.44	7.85
  14	BHLHB2	10.75	6.64			6.64	10.96
  15	LAMC1	9.85	5.80	5.80	9.67
  16	CD69	13.27	9.40			9.40	12.27
  17	PLEKHA1	10.95	7.20
  18	PTGDR	7.20	3.46
  19	TXNIP	14.89	11.20	11.20	13.39	11.20	15.10
  20	SIDT1	11.52	7.86	7.86	9.38
  21	LY6E	8.57	4.92	4.92	7.39
  22	IGF1R	8.73	5.13
  23	PRICKLE1	11.68	8.09	8.09	9.69
  24	CSDA	12.12	8.59	8.59	10.10
  25	KLF2	12.61	9.10	9.10	11.70
  26	ELOVL2	7.32	3.86
  27	CCND2	12.06	8.61			8.61	11.53
  28	LASS6	10.29	6.86	6.86	9.42	6.86	9.48
  29	DUSP6	11.93	8.53			8.53	10.19
  30	CUGBP2	14.28	10.93			10.93	14.29
  31	PTPRO	9.64	6.32			6.32	8.63
  32	PHF16	10.33	7.04
  33	NR3C2	8.85	5.58
  34	CRTC3	12.56	9.32	9.32	11.11
  35	ADAMTS6	7.68	4.45
  36	ETV6	10.36	7.14	7.14	8.89	7.14	10.43
  37	LRRC17			3.10	7.49
  38	FAM46C	12.18	8.98	8.98	14.76	8.98	10.54
  39	SATB2	7.20	4.02	4.02	7.60
  40	RNF125	9.00	5.83
  41	ST6GALNAC3	8.12	4.96
  42	LARGE	9.05	5.89	5.89	8.73
  43	ZNF276	9.39	6.25
  44	KCNA3			4.83	9.82
  45	BCL2	10.89	7.76			7.76	11.41
  46	MTSS1	12.92	9.82			9.82	12.05
  47	NR6A1	9.10	6.03			6.03	7.62
  48	BHLHB3	11.11	8.05	8.05	13.26	8.05	13.26
  49	MYO10	7.22	4.19
  50	ITM2C			6.44	13.85
  51	C18orf1	9.49	6.47	6.47	8.63
  52	FXYD7	7.36	4.35
  53	DUSP8	7.96	4.96	4.96	8.41	4.96	7.75
  54	BTBD3	7.41	4.42
  55	TMEPAI	11.15	8.20	8.20	11.03
  56	ANTXR2	9.90	6.97
  57	FOSB	10.13	7.21	7.21	11.05
  58	TMCC3	8.23	5.31	5.31	7.09	5.31	8.05
  59	ARL4C	11.31	8.41			8.41	10.32
  60	ZMYND11	11.33	8.43	8.43	11.34	8.43	11.68
  61	RHOBTB1	7.33	4.48
  62	JUN	12.14	9.34	9.34	12.70
  63	SKI	10.46	7.67			7.67	10.68
  64	TMEM121	7.07	4.29
  65	IL13RA1	8.99	6.21
  66	KIF13B			6.23	9.01
  67	BHLHB5			4.26	7.39
  68	PGM2L1	10.35	7.65
  69	C14orf4	12.11	9.47	9.47	11.01	9.47	11.65
  70	PDE4B	12.38	9.74	9.74	11.60
  71	PDE7B	8.64	6.03
  72	BCL9L	9.37	6.77	6.77	8.61
  73	PCDH9	10.32	7.73
  74	ARHGAP5	10.44	7.86	7.86	9.11	7.86	9.85
  75	KIAA0802	9.18	6.61	6.61	7.91	6.61	7.89
  76	ITGB4	7.48	4.91
  77	NOTCH2NL			7.17	8.95
  78	CDC42BPA	7.32	4.77
  79	SPG20	9.25	6.73
  80	KLF4	10.04	7.57	7.57	9.09
  81	AHNAK	9.34	6.87	6.87	9.56	6.87	11.51
  82	FCHSD1	9.15	6.69
  83	KIAA1622			3.94	7.67
  84	PLXNC1	10.92	8.48
  85	TMEM150	8.39	5.96
  86	ACVR2A	7.81	5.38	5.38	8.63	5.38	7.40
  87	KALRN			5.61	7.64
  88	ANK2	7.69	5.28	5.28	7.54	5.28	7.00
  89	FLJ14213	7.61	5.21	5.21	7.97
  90	CHPT1	10.92	8.52
  91	TCF2			5.69	7.82
  92	FGF5			5.41	7.52
  93	SLC12A6	12.18	9.80	9.80	11.09
  94	MGC17330	11.53	9.16
  95	NR4A2	10.21	7.85	7.85	9.21	7.85	10.54
  96	SLC39A10	11.06	8.70
  97	LITAF	12.52	10.18	10.18	11.82
  98	AKT3	9.32	6.99			6.99	9.25
  99	PDCD4	12.89	10.57			10.57	13.33
  100	STMN3	10.03	7.72
  101	SIDT2	13.13	10.85
  102	GRIA3	7.04	4.77
  103	EML4	12.83	10.57
  104	DIP2B	11.26	8.99			8.99	10.77
  105	FBN1			4.89	8.17
  106	FAM84B	9.21	6.96
  107	EGR3	11.59	9.35	9.35	8.24	9.35	10.62
  108	CTHRC1			4.25	11.94
  109	RRAGD	7.69	5.46
  110	MACF1	12.88	10.66
  111	FOXF2	7.05	4.83
  112	GAB1	8.01	5.81	5.81	10.91
  113	ST18			4.42	7.40
  114	ZFP36L2	13.78	11.59	11.59	13.43	11.59	14.05
  115	GAB2	8.29	6.12	6.12	9.02
  116	CHST11	11.17	8.99	8.99	10.28
  117	CLOCK	9.85	7.68	7.68	9.91	7.68	9.62
  118	PREX1	10.07	7.90			7.90	11.16
  119	KLF11	8.08	5.94			5.94	8.39
  120	PTGER4	11.66	9.53
  121	KLF9	9.47	7.34			7.34	9.27
  122	FLJ37078	7.55	5.42	5.42	7.49
  123	ODZ2	7.20	5.08	5.08	7.25	5.08	7.22
  124	SESN3	11.24	9.11	9.11	7.60
  125	PDK4	7.22	5.09
  126	CNTNAP3	7.35	5.23	5.23	7.10
  127	DUSP1	13.34	11.22
  128	MARCKS	11.19	9.07	9.07	11.05	9.07	12.21
  129	SPRY4	7.02	4.91
  130	LMO1			4.75	7.16
  131	MTUS1	7.77	5.66	5.66	8.16
  132	ADCY9	8.01	5.91	5.91	7.74
  133	SLC17A6			3.80	7.06
  134	NOX4	7.45	5.37	5.37	7.22
  135	UTRN			9.46	10.97
  136	ZBTB10	8.50	6.45
  137	SLC26A7	8.37	6.32	6.32	7.89
  138	PNRC1	13.14	11.09
  139	LLGL2			6.83	9.29
  140	CHST1	7.15	5.12
  141	CREB3L2	10.53	8.51	8.51	13.30
  142	DDIT4	12.69	10.68
  143	C20orf108	8.82	6.80	6.80	10.19
  144	CDH1			3.85	10.26
  145	TFAP4	7.28	5.29
  146	SLC38A2	13.57	11.58	11.58	13.74	11.58	13.17
  147	SESN1	9.84	7.87	7.87	9.13
  148	YPEL2	10.18	8.20			8.20	10.04
  149	GRASP	8.47	6.50
  150	TSC22D3	13.22	11.26	11.26	12.67
  151	ATP11A	9.70	7.75	7.75	9.50
  152	L3MBTL3	10.61	8.66			8.66	10.06
  153	SORT1	7.61	5.67	5.67	8.11
  154	CAV1			4.80	9.11
  155	RXRA	9.07	7.15	7.15	8.81
  156	CRELD1	8.40	6.48	6.48	8.26
  157	RBMS1	12.40	10.49	10.49	8.80
  158	LYST	10.90	8.99
  159	PIP5K1B	9.61	7.70	7.70	9.97
  160	JUNB	11.62	9.72
  161	MBOAT1					4.80	7.27
  162	IRF4	11.16	9.28	9.28	13.66
  163	LIFR			4.62	7.08
  164	MORC3	11.25	9.37
  165	MBP	11.91	10.05	10.05	8.61
  166	SRC			4.98	7.98
  167	ALS2CR13	11.79	9.93	9.93	8.89	9.93	11.47
  168	MYH1			4.40	7.37
  169	DUSP3	9.36	7.50	7.50	9.16
  170	HLX1	7.14	5.28			5.28	7.65
  171	CDKN1A	9.09	7.23	7.23	8.97	7.23	8.43
  172	SOCS5	10.00	8.15
  173	PPP1R9A			5.14	7.35
  174	TGFBR2	12.81	10.99			10.99	12.63
  175	LRRC16	8.47	6.66
  176	ZNF629	8.17	6.35	6.35	8.58
  177	RPS6KA5	11.75	9.95			9.95	11.48
  178	SATB1	12.79	10.99	10.99	9.06
  179	SEMA4C	7.38	5.59	5.59	8.49	5.59	7.14
  180	ULK1	7.79	5.99	5.99	8.72	5.99	8.06
  181	STX3	8.88	7.09	7.09	8.91
  182	BAMBI					4.81	7.26
  183	MAP3K5	10.63	8.84	8.84	7.66	8.84	10.71
  184	KIAA1147	11.70	9.91			9.91	11.53
  185	SBK1			5.20	7.04
  186	RYR3	7.80	6.02	6.02	8.27
  187	ZNF238	13.05	11.27	11.27	10.08	11.27	12.54
  188	IL12A			5.65	7.26
  189	SLC2A3	12.00	10.23			10.23	11.89
  190	GFPT2			3.39	7.18
  191	G0S2	7.72	5.96
  192	LYRM5	10.29	8.53	8.53	10.93
  193	SSH2	11.67	9.91			9.91	11.85
  194	NRP1	7.44	5.69	5.69	7.22
  195	LMLN	8.92	7.19
  196	UCP3	7.71	5.99
  197	TMEM166	7.31	5.60
  198	CACNA1I	7.46	5.76	5.76	7.06
  199	PHOSPHO1			3.55	7.10
  200	CRIM1	9.79	8.11			8.11	9.32
  201	GATA6			5.41	7.59
  202	SACS	10.06	8.39			8.39	10.54
  203	CDKN1B	12.91	11.24	11.24	14.41
  204	CACNA2D2			5.25	7.57	5.25	7.93
  205	MKRN3			3.65	7.10
  206	MTFR1	10.40	8.74	8.74	7.34	8.74	10.15
  207	GALNT3	10.19	8.54
  208	RPS6KA3	12.07	10.42			10.42	11.48
  209	DTNA			5.47	7.86
  210	MAGI2	7.04	5.40	5.40	7.42
  211	FOXJ2	10.01	8.38	8.38	9.97	8.38	9.85
  212	KIAA0513			5.29	8.44	5.29	7.36
  213	NDRG1			7.75	10.02
  214	AKAP7	10.16	8.54
  215	CD72	13.74	12.12
  216	IGFBP5	7.30	5.69	5.69	7.59
  217	REPS2	7.51	5.92	5.92	7.60
  218	PRDM12	7.17	5.57
  219	ZNF3	8.86	7.27	7.27	8.75
  220	TLL2			4.88	7.01
  221	PCNX	9.77	8.19
  222	ARHGAP24	11.31	9.73	9.73	8.65	9.73	11.32
  223	THRAP2	11.31	9.73
  224	RNF11	9.72	8.15	8.15	11.08
  225	HOXC8	7.05	5.48
  226	SCML2	10.33	8.76
  227	BMPR2	9.70	8.14			8.14	10.06
  228	STAC			5.27	7.20
  229	C10orf54	9.12	7.56	7.56	10.16
  230	FBXL17	9.51	7.95	7.95	9.36
  231	CBX7	10.87	9.32			9.32	10.96
  232	UBE2W	9.80	8.25	8.25	9.76	8.25	9.38
  233	ProSAPiP1			4.12	7.54
  234	UBL3	10.73	9.18	9.18	11.27	9.18	11.03
  235	TTYH3	7.35	5.81	5.81	8.16
  236	PUNC			5.39	8.85	5.39	7.30
  237	GDF11	7.01	5.47	5.47	7.70
  238	LMX1A			5.25	7.43
  239	TIMP2	7.51	5.97	5.97	7.69	5.97	7.40
  240	NHLH2			3.93	7.35
  241	CLCF1	10.50	8.98
  242	ITGB3	7.66	6.14
  243	TMEM132E			3.93	7.20
  244	KCTD17	7.45	5.94	5.94	7.33
  245	ChGn	7.75	6.24	6.24	8.36
  246	DIP2C	9.25	7.75
  247	DKFZp667G2110	8.79	7.28	7.28	9.25
  248	CSNK1G3	10.84	9.34			9.34	10.70
  249	NRIP1	11.79	10.30
  250	SMAD3	10.72	9.23	9.23	7.74	9.23	10.54
  251	SHOX2	7.05	5.57
  252	LDLRAP1			6.80	9.84	6.80	8.59
  253	SUPT3H	7.77	6.30	6.30	8.05
  254	KCNN2	7.18	5.70
  255	DLL1	7.37	5.90
  256	CEND1	7.72	6.26
  257	NOTCH1	10.90	9.45
  258	TLE1			7.74	9.63
  259	FCHO2	8.75	7.30
  260	JPH4	7.12	5.67	5.67	7.60
  261	KCNMB2	7.16	5.72
  262	KHDRBS2	7.17	5.73
  263	LEFTY1			4.38	7.21
  264	ST3GAL5	10.59	9.15
  265	LGR4			5.30	7.29
  266	FNDC8			4.25	8.01
  267	NPTXR			5.87	7.65
  268	PRICKLE2			5.47	7.47
  269	GRIA4			5.19	7.04
  270	RHOB	8.66	7.23	7.23	9.56
  271	ZADH2	8.65	7.22
  272	ZBTB41	9.73	8.31			8.31	9.42
  273	GPRASP2			6.00	7.35
  274	SYS1	10.79	9.36
  275	RUNX1T1	7.37	5.95
  276	DLX2			4.66	7.04
  277	SLC30A7	11.58	10.17
  278	PER1	8.82	7.40
  279	NT5C3	12.67	11.25
  280	PDE3A	11.62	10.21
  281	OCRL	8.07	6.66			6.66	8.04
  282	PSD3	7.99	6.60
  283	LPHN1	7.80	6.41
  284	TNRC6B	11.87	10.48			10.48	11.77
  285	SNN	12.03	10.64	10.64	8.91	10.64	12.10
  286	HERPUD2	11.56	10.18
  287	UBQLNL	7.45	6.07
  288	HES7	7.37	5.99
  289	GALNT2	10.08	8.69	8.69	10.63
  290	CAMKK1	8.45	7.07
  291	ELN			5.44	7.18
  292	ICK	8.09	6.72	6.72	8.01
  293	POU4F2	7.09	5.72	5.72	7.21
  294	GAS2	7.13	5.76	5.76	7.81
  295	ARHGEF3	10.11	8.75			8.75	11.04
  296	ZBTB4	11.45	10.09			10.09	11.78
  297	CHD7	12.14	10.78			10.78	12.08
  298	TMEM45B			5.97	7.61
  299	CLDN11			5.74	7.22
  300	PTPN1	10.85	9.51
  301	PHF20	12.00	10.67
  302	VAV3			10.44	7.84
  303	CARKL	8.38	7.06	7.06	8.94
  304	TRIM36			5.43	7.77
  305	CTLA4			6.57	7.95
  306	POLK	9.69	8.38	8.38	10.55
  307	WSB1	13.22	11.91			11.91	13.02
  308	ALS2CR2	9.87	8.57	8.57	10.14
  309	PLK2			4.94	7.13
  310	SRPK2	10.83	9.53			9.53	11.13
  311	ARRDC2	10.86	9.55
  312	IER5	13.10	11.80
  313	EPN1	8.95	7.66	7.66	9.07
  314	SLC20A2	9.45	8.16
  315	DLG4			4.23	7.22
  316	TMTC2			6.16	7.92
  317	ETV1	8.19	6.90
  318	JAZF1	11.93	10.65	10.65	7.49
  319	VAV2	10.22	8.94
  320	C15orf27	7.19	5.91
  321	FYCO1	9.75	8.47
  322	KIAA0789	7.27	6.00
  323	OBFC2A	9.94	8.66			8.66	10.94
  324	MCF2	7.19	5.92
  325	KIAA2018	11.77	10.50	10.50	9.50
  326	MTMR10	10.75	9.48
  327	FAM63B			6.03	7.83	6.03	7.95
  328	SNF1LK	10.20	8.93			8.93	10.18
  329	ZNF385	8.89	7.63
  330	SESTD1	11.05	9.78
  331	SLC31A2			5.41	9.13
  332	PCMTD1	12.03	10.76	10.76	12.44
  333	NBEA	8.06	6.81	6.81	8.02	6.81	8.35
  334	ZNF295			9.24	10.61
  335	SIPA1L3	10.81	9.57
  336	CC2D1B	9.11	7.87
  337	PRKAG2			7.12	8.42
  338	PKD1	9.04	7.81
  339	CNTNAP2	7.54	6.32
  340	FNBP1L	7.90	6.67
  341	HEXIM1	10.27	9.05			9.05	10.24
  342	C19orf2	12.29	11.07
  343	MYLIP	11.62	10.41			10.41	11.81
  344	SLC11A2	9.27	8.06
  345	CLU	9.92	8.71
  346	GCN5L2	10.18	8.97
  347	DKFZP564J0863	10.85	9.65			9.65	11.11
  348	CNNM2	7.85	6.64	6.64	8.07
  349	CDC42SE1	12.07	10.87			10.87	12.18
  350	HOXB9			6.20	8.41
  351	NFATC3	11.39	10.19
  352	UNC84B	11.12	9.93	9.93	8.59
  353	DUSP5			6.23	9.44
  354	ING1	11.35	10.17
  355	ITM2B			11.23	13.62
  356	FAM53B	11.67	10.49
  357	ZFP36L1	14.07	12.89	12.89	10.09
  358	NIPA1	9.75	8.57			8.57	10.07
  359	GALNT1	10.93	9.76	9.76	11.12
  360	MYPN			4.25	7.32
  361	ITPK1	10.03	8.86
  362	TTYH2	7.90	6.73
  363	DOCK10	10.80	9.63			9.63	11.53
  364	C14orf28	9.18	8.01	8.01	9.58	8.01	9.50
  365	RP11-130N24.1			4.76	7.62
  366	FGF12			5.82	7.33
  367	ATP2B2	7.93	6.76
  368	PPP1R9B	9.77	8.60
  369	PPP3CA	13.50	12.33			12.33	13.86
  370	TFAP2A			5.54	8.26
  371	CYLD	10.91	9.75
  372	PHF1	11.85	10.69	10.69	12.26
  373	NEBL	7.98	6.82	6.82	8.08
  374	ACIN1	12.04	10.89			10.89	11.99
  375	SPTBN1	12.28	11.14	11.14	12.53	11.14	12.42
  376	VAMP4	10.81	9.67	9.67	11.18
  377	DNAJB9	10.80	9.66	9.66	15.21	9.66	11.03
  378	ZDHHC2	11.55	10.42
  379	TRIO	10.57	9.43
  380	TMEM25			6.24	8.57
  381	TAF9B	10.82	9.69
  382	ARID3A	8.03	6.90	6.90	9.81
  383	KIAA0182	10.68	9.55
  384	RPS6KA2	8.61	7.49
  385	C3orf58	11.47	10.34
  386	CAST	11.94	10.82
  387	SH3PXD2A	8.46	7.34
  388	RAB6B			5.93	7.36
  389	RNF141	11.47	10.35
  390	SP4	11.26	10.14
  391	ARMCX2	9.44	8.32	8.32	9.54
  392	ZNF398	10.39	9.28
  393	PBX3	9.69	8.58	8.58	7.00
  394	FOS	12.35	11.25	11.25	13.34
  395	FHOD3			6.40	7.94
  396	C20orf59	10.11	9.01	9.01	10.92
  397	FAM117A	11.67	10.57	10.57	12.06	10.57	11.64
  398	ATP8B2	8.58	7.49	7.49	10.87	7.49	8.88
  399	UXS1	9.53	8.43
  400	GOLGA8A	12.84	11.74	11.74	13.14	11.74	13.05
  401	SUFU			6.03	7.84
  402	NAGPA	9.32	8.23
  403	MLLT6	11.70	10.61
  404	CPEB4	9.46	8.36	8.36	12.21
  405	TMEM50B	10.67	9.58	9.58	11.41	9.58	10.63
  406	AMPD3	11.65	10.56	10.56	8.76
  407	SIX5			5.19	7.53
  408	MMP16			5.66	7.47
  409	LBH	11.87	10.79	10.79	8.83
  410	POM121					9.60	11.26
  411	ATP8B1	11.93	10.86	10.86	8.88
  412	ARHGAP29			3.75	7.60
  413	ZNF395			11.78	10.76
  414	CRY2					6.50	8.01
  415	BIN1			10.68	9.67
  416	PSAP			11.24	13.41	11.24	12.71
  417	ISL1			5.91	7.60
  418	DNAJB5	8.04	6.97
  419	KIAA0284			6.42	8.79
  420	RBM35B	11.44	10.38
  421	ADCY7	11.53	10.47
  422	ARRDC3					9.46	10.68
  423	MICAL1	11.72	10.67
  424	MTHFR	10.31	9.26
  425	HOOK3	8.57	7.53	7.53	6.52
  426	EFNB3			5.16	7.79
  427	ARHGAP12	9.86	8.82
  428	LMBR1L	9.33	8.29
  429	FGF7			5.88	7.86
  430	USP2			5.50	7.52
  431	SMARCA2	11.14	10.12
  432	ELL2			8.23	13.60
  433	C1orf26			7.06	10.01
  434	CXCR4			14.61	13.45
  435	HDGFRP3			5.58	7.39
  436	BNC2			6.18	7.72
  437	YPEL3			10.16	11.81	10.16	11.47
  438	PPFIA3			4.58	8.06
  439	DLGAP4			9.34	10.68
  440	ZBTB7A					9.84	11.10
  441	D4S234E			5.90	7.38
  442	FNDC3A			9.41	13.60
  443	FOXC1			5.84	7.39
  444	KIF26B			5.55	7.30
  445	NAV2			6.26	7.90
  446	DDX3Y			8.07	10.38
  447	FGD1			7.31	6.06
  448	HTR4			7.26	9.01	7.26	8.40
  449	C22orf31			5.67	8.10
  450	RNF44			11.48	10.14
  451	GALNAC4S-6ST			10.43	12.71
  452	CCNT2					10.15	11.23
  453	CHIC1					8.91	10.02
  454	NNAT			5.97	8.19	5.97	7.84
  455	CTDP1			6.81	8.26
  456	BTG1			14.95	13.94
  457	CMTM4			5.27	7.81
  458	GOLGA8B					11.37	12.63
  459	HBP1			10.07	12.13
  460	GDPD1					6.35	7.61
  461	CDYL			9.31	10.61	9.31	10.50
  462	ZNF217			10.77	9.73
  463	KIF5A			6.13	7.75
  464	C1QL1			5.80	8.11
  465	SOLH					7.88	9.03
  466	ZC3H6					9.81	10.96
  467	LATS2			7.61	10.19
  468	COL18A1			6.46	7.85
  469	C11orf24			9.89	11.07
  470	SCUBE3			6.14	7.88
  471	SEMA4G			5.57	7.81
  472	FOXP1			12.31	10.84
  473	TRIB2			10.62	9.61
  474	LOC285382			5.35	7.20
  475	RUNX2			7.91	10.22
  476	LOC196463			4.16	7.52
  477	LPGAT1			10.57	7.99
  478	RASSF2			12.52	10.93
  479	IRF1			9.86	11.60
  480	RAB40B			7.31	10.89
  481	CTDSPL			6.37	7.83
  482	CLCN4					7.44	6.39
  483	CACNB1			6.41	8.20
  484	SYNGR1			8.45	10.40
  485	ST8SLA4					10.67	11.81
  486	PLD3			7.63	10.59
  487	FOXO3A			10.17	12.41
  488	TSPAN33			10.49	9.38
  489	HIST1H4F			6.20	8.08
  490	LAPTM4A					12.05	13.06
  491	PRKCB1			12.14	10.50
  492	PROX1			6.38	7.89
  493	CDK5R1			10.12	7.78
  494	MAP3K9			7.96	9.13
  495	GPX3			5.92	7.61
  496	GNS			8.79	11.06
  497	ARL15			8.27	6.51
  498	OXR1			9.21	11.17	9.21	10.25
  499	AAK1			7.38	9.54	7.38	8.47
  500	SH3PX3			6.72	8.58
  501	MS4A7			10.55	8.35
  502	FLJ20273			5.96	11.23	5.96	8.65
  503	ISCU			12.01	13.55
  504	ITGA2			5.70	7.60
  505	ME1			5.87	7.89
  506	LRP1			4.92	7.04
  507	ZNF652					9.66	10.81
  508	TRAK1			11.07	9.85
  509	SLC8A1			6.27	7.83
  510	C1orf119					10.76	11.98
  511	KLF6			11.33	9.24	11.33	12.40
  512	TRIM2			6.22	8.20
  513	USP3			11.11	12.51
  514	ARID5B					11.02	12.48
  515	RASD1			6.15	9.32
  516	ZCCHC2			10.63	8.93
  517	LEFTY2					5.08	7.03
  518	BACH1			9.97	11.76
  519	IRAK1			11.48	12.80
  520	RP11-217H1.1			10.76	13.04
  521	HLCS			6.54	8.45
  522	NAGK			10.94	12.14
  523	CELSR2			6.26	7.89
  524	PCBP4			7.75	9.08
  525	FLJ25476					9.49	10.72
  526	TPP1			12.10	11.06
  527	ACVR1			7.95	9.31
  528	EHD3					8.99	10.35
  529	FAM80B			9.35	8.02
  530	SPRYD3			8.19	9.51
  531	PRDM4					9.81	10.83
  532	C6orf134			7.92	5.60
  533	HSP90B1			12.04	16.36
  534	DYNC1I1			5.98	7.33
  535	NFLX			7.43	8.67
  536	DOCK4					5.96	7.97
  537	ZNF287			7.09	8.47
  538	XRN1			10.92	12.37
  539	YES1			6.39	8.60
  540	RBM35A			8.19	9.44
  541	HOXB4			7.58	9.27
  542	OTUD7B			7.24	8.57
  543	CELSR3					6.83	8.39
  544	RHOC					7.71	8.84
  545	ZNF607			8.10	6.95
  546	XYLT1			10.28	8.78	10.28	8.86
  547	FAM89B			9.16	10.75
  548	OSBPL8			12.99	11.66
  549	SRCRB4D			6.82	8.67
  550	RASL12			5.05	8.07
  551	NAG8			8.39	9.78
  552	MAN1A2			10.83	12.15
  553	PPARA					8.59	9.65
  554	CLDN12			6.66	8.39
  555	ID4			5.93	7.79
  556	HECTD2			7.98	6.80
  557	EFCAB4A			5.93	8.96
  558	CREBL1					6.97	8.09
  559	SARM1					6.98	8.29
  560	MIDN			8.48	7.29
  561	EMB			10.48	8.65
  562	FOXK2			8.40	9.63
  563	INADL			6.87	8.44
  564	KCNAB1			6.26	9.76
  565	MAP3K8			10.92	9.60
  566	C8orf58			7.89	5.62
  567	SLC4A7			9.82	8.52	9.82	10.90
  568	RAB8B			12.46	10.92
  569	C10orf118			8.93	10.20
  570	CRTC2			9.37	7.94
  571	KLC2			7.31	9.19
  572	SRCAP			8.06	6.96
  573	CNTFR			7.15	8.67
  574	SPTBN2			4.75	7.11
  575	SMAD7					8.69	9.93
  576	NDFIP1			9.10	10.95
  577	BCL11A			14.03	10.63
  578	SFXN5			7.26	8.87
  579	RIMBP2			5.93	8.33
  580	FUT8			8.62	11.08
  581	PSEN2			5.86	9.17
  582	MAP6			5.22	7.37
  583	FOXO1A			11.53	10.14
  584	BTG2			11.81	13.82
  585	C10orf56			6.93	9.30
  586	MAPK1			12.06	10.36
  587	ZBTB47			6.63	8.12
  588	GOSR2			9.73	11.07
  589	ZFP90			10.15	8.92
  590	RALGPS1			8.02	9.38
  591	DGKI			4.77	7.39
  592	RGL2			11.08	9.55
  593	PAK6			5.90	8.55
  594	DMXL1			11.85	10.31
  595	TMEM113			12.28	11.03
  596	SNX24			6.75	8.53
  597	HOXA3			5.99	8.27
  598	SAMD4A			9.84	8.36
  599	WDR45			9.88	12.43
  600	TLOC1			12.91	14.52
  601	LARP2			7.64	11.50
  602	DTX2			7.60	6.20
  603	ITGA9					7.53	6.32
  604	FASTK			10.04	11.22
  605	TAOK2			7.47	6.37
  606	CRTAP					9.78	11.08
  607	SERTAD2			11.91	10.82
  608	TBP			10.14	9.09
  609	IQGAP2			7.26	11.19	7.26	8.93
  610	RASGEF1B			6.23	8.01
  611	PIP5K2B			9.69	8.53
  612	PRRC1			10.32	11.92
  613	RHPN2			6.73	8.21
  614	DYRK1B			5.92	7.61
  615	ADCY2			5.98	7.97
  616	C12orf34			5.61	7.09
  617	GMPR			5.35	8.15
  618	PAK2			10.86	9.66
  619	KIAA1539			8.12	9.49
  620	PLAGL1			10.03	6.68
  621	LCORL			9.74	11.29
  622	BCL9			7.21	9.16
  623	CPEB2					9.09	10.23
  624	TMEM59			12.10	14.75
  625	TRIP10			7.17	9.36
  626	CCPG1			8.38	11.86
  627	NDE1			10.71	9.30
  628	ZDHHC21			9.38	8.11
  629	STOX2			5.87	7.66
  630	RAB4B			10.63	8.66
  631	LRRFIP1			13.25	11.99
  632	OSBP			10.10	11.39
  633	RAB6A			10.76	12.27
  634	RHOV			4.88	7.19
  635	SLC39A14			8.80	11.00
  636	ADARB1			10.05	7.69
  637	ESPN			6.85	8.19
  638	SEC31A			11.64	13.61
  639	PJA2			10.85	11.99
  640	SYNGR3			9.01	7.56
  641	LOC339745			11.94	10.54
  642	NEDD9			11.23	12.48
  643	COL23A1			5.41	7.02
  644	PIP5K3			11.93	10.79
  645	PTPRG			7.30	8.49
  646	DAB2IP			5.61	8.30
  647	MAPRE3			5.80	8.60
  648	SQSTM1			10.05	11.45
  649	RAB6C			10.88	12.04
  650	FAM57A			7.58	6.18
  651	YPEL5			12.41	13.78
  652	TTN			9.61	7.03
  653	HTRA3			5.62	8.02
  654	CHST2			7.80	12.49
  655	BAZ1A			11.43	10.06
  656	LRFN5					7.38	6.21
  657	MBNL1			14.10	12.62
  658	MLL2			10.93	9.89
  659	SF1			11.44	10.36
  660	FRMPD1			4.80	7.06
  661	PPP2R5B			7.60	9.12
  662	RNF43			10.04	8.01
  663	GAP43			5.07	7.49
  664	NOMO1			11.11	14.31
  665	VEGFA			7.79	9.36
  666	C22orf5			8.75	11.38
  667	AKAP11			11.79	10.64
  668	INSIG2			9.46	11.32
  669	PDIK1L			9.11	10.72
  670	TMEM4			10.93	13.32
  671	LMNA			7.80	9.75
  672	TP53INP1			11.84	14.14
  673	NAV1			6.96	8.31
  674	SPTY2D1			9.25	10.77
  675	CREBL2			9.95	11.10
  676	MFAP3L			5.90	7.46
  677	REXO2			10.96	13.71
  678	SPTBN4			6.77	8.22
  679	NOMO2			9.74	12.94
  680	PTEN			12.78	11.40
  681	FBXL16			6.86	9.56
  682	GPM6A			7.87	6.15	7.87	6.67
  683	PRX			6.76	8.91
  684	HOXC11			6.40	7.88
  685	KIAA0329			8.27	9.87
  686	PAFAH1B1			11.68	10.33
  687	C20orf174			9.58	7.27
  688	MTPN			13.87	12.17
  689	TMED5			12.25	14.41
  690	FURIN			8.03	9.54
  691	ELAVL3			4.66	8.01
  692	SH2B3			10.31	8.87
  693	LIN28B			5.99	7.58
  694	KIAA1033			12.65	11.38
  695	TMEM28			4.95	7.14
  696	TBC1D15			10.30	11.52
  697	GOLGA4			10.11	11.71
  698	STX1A			5.63	7.82
  699	SLC40A1					6.87	8.27
  700	KIAA1815			9.18	7.93
  701	HNT			5.53	7.09
  702	PDE11A			5.72	7.82
  703	TGFBR3			7.84	9.52
  704	MTF1			9.92	8.86
  705	MAN1A1			9.99	13.65
  706	THRA			7.26	8.48
  707	CBLB			11.46	9.08
  708	SLC35F1			6.29	8.16
  709	FOXP4			8.52	7.30
  710	ELOVL5			13.83	12.78
  711	NMUR1			4.13	7.31
  712	ITPR1			12.06	9.69
  713	CDCA7L			11.95	10.33
  714	RASSF1					9.35	10.69
  715	IL1RAPL1			6.42	8.18
  716	AUH			9.37	11.19
  717	DIAPH1			11.52	9.74
  718	ENSA			12.33	11.22
  719	FAM43A			10.62	9.39
  720	CSMD3			4.79	7.58
  721	SLC25A37			10.10	8.69
  722	SLC33A1			9.78	12.19
  723	CNOT6			9.98	8.93
  724	GRID1			7.05	8.80
  725	C21orf91			11.51	10.18
  726	ITPKB			11.28	8.91
  727	CP			5.89	7.41
  728	HMBOX1			10.05	8.50
  729	ARID1A			12.22	11.11
  730	C5orf5			11.86	10.38
  731	TMSB4X			16.86	15.10
  732	EPAS1			7.02	8.39
  733	HPS3			11.65	10.16
  734	DKK1			6.16	7.86
  735	ZNF282			7.89	5.40
  736	AP3D1			11.29	12.84
  737	DERL1			11.69	13.43
  738	C5orf13			9.89	7.62
  739	CCNG2			13.32	11.64
  740	PGRMC2			9.44	11.38
  741	ARF6			13.60	12.55
  742	TMEM110			7.75	9.24
  743	FAM13A1			7.11	9.69
  744	BRMS1L			8.58	10.18
  745	TRAM1			12.52	15.17
  746	CALU			9.42	11.20
  747	GOLPH3			11.55	12.81
  748	MAP3K7			11.14	10.06
  749	ABCG4			6.90	8.22
  750	NELF			8.75	7.70
  751	ADIPOR1			9.67	10.92
  752	INSR			8.48	11.62
  753	GRM5					6.24	7.65
  754	TFG			10.76	12.30
  755	USP48			11.91	13.09
  756	CLCN6			9.26	7.48
  757	ZNF219			6.11	8.42
  758	C7orf43					8.34	6.62
  759	NFKB1			11.90	10.70
  760	ARF3			11.11	9.87
  761	JMJD1C			12.34	11.11
  762	ROD1			13.13	11.42
  763	OXSR1			10.69	9.15
  764	ERGIC2			10.86	12.81
  765	EIF4E3			7.61	9.87
  766	SEC24A			10.05	12.85
  767	SPRED1			7.19	5.77
  768	HNRPH3			12.60	11.38
  769	ZDHHC7			10.34	9.05
  770	HNRPA1			16.14	15.14
  771	TMED2			12.01	14.26
  772	VASP			10.60	8.60
  773	CHD4			11.40	10.31
  774	RTN4RL1			5.48	8.36
  775	PPP1R12A			12.03	10.97
  776	SGK3			9.58	11.68
  777	ARHGAP17			12.37	8.62
  778	GORASP2			11.28	13.30
  779	C2orf44			8.38	6.72
  780	FAM116A			11.12	10.08
  781	SSR1			12.75	14.73
  782	MYH9			11.24	9.89
  783	PIK3CD			12.99	10.03
  784	DAZAP2			14.26	13.23
  785	SEL1L			10.74	14.04
  786	LOC388284			7.05	8.32
  787	THRB			7.51	6.11	7.51	6.29
  788	MGAT2			11.91	14.44
  789	C8orf13			9.35	6.47
  790	PDZD2			7.00	4.39
  791	DERL2			10.74	12.75
  792	ZCCHC5					7.39	6.27
  793	NFYC			10.27	9.03
  794	GLUD2			8.88	7.58
  795	ESR1			7.55	9.88
  796	NRP2			6.51	8.13
  797	NUS1			10.01	11.53
  798	BIRC6			12.15	10.94
  799	ARID3B			7.83	9.22
  800	STCH			10.16	13.88
  801	SLC7A11			6.32	8.00
  802	OSBPL5			7.91	9.22	7.91	6.62
  803	IGFBP3			5.60	7.61
  804	LNPEP			13.72	11.05
  805	ZNRF1			8.18	9.68
  806	DDAH1			7.96	5.87
  807	ERG			4.07	7.49
  808	APLP2					10.90	8.38
  809	ZNFX1			10.14	8.93
  810	GPM6B			8.66	6.69
  811	BLCAP			11.49	10.10
  812	SRP68			11.47	12.88
  813	PIK3AP1			14.12	11.74
  814	ANKRD28			11.00	12.46
  815	KCNH8			8.27	4.80
  816	KIAA0430			12.43	10.61
  817	PPP2R1B			9.84	8.41
  818	TMED9			11.62	13.82
  819	CTNNBIP1			7.31	8.89
  820	PPP2R5D			9.51	7.92
  821	CBX4			12.36	13.69
  822	AKAP6			7.38	5.55	7.38	4.93
  823	C2orf30			10.72	13.84
  824	DR1			11.97	10.65
  825	MTDH			12.46	13.96
  826	ETS1			14.40	12.62
  827	EPHA8			6.12	8.10
  828	ANKRD13B					7.23	5.85
  829	C4orf16			8.83	7.70
  830	SOX4					9.14	10.20
  831	SLC12A2			10.23	9.01
  832	IER3IP1			11.35	12.56
  833	DST			7.02	8.34
  834	WRNIP1			11.03	9.49
  835	CLCC1			9.63	11.48
  836	B4GALT6			7.90	6.82
  837	BTBD10			10.12	8.07
  838	SDC1			7.05	12.14
  839	C10orf12			10.32	8.40
  840	ZDHHC3			9.80	8.78
  841	GNAI2			10.86	9.27
  842	HNRPU			13.82	12.47
  843	GNAZ					8.86	7.26
  844	ALG9			8.93	10.97
  845	FBXL10			11.75	10.43
  846	ARID4B			12.19	10.96
  847	PPP1R3F			6.19	7.79
  848	EPB49			6.49	7.94
  849	PCNP			12.91	11.74
  850	PCYT1B			11.35	9.77
  851	RAB14			11.64	10.41
  852	TCERG1			12.50	11.46
  853	MKNK2			14.87	12.67
  854	COL4A3					8.78	12.23
  855	HNRPA0			12.50	11.36
  856	P2RX4			8.88	10.29
  857	JUP			9.23	5.70
  858	EGR2			10.43	8.07
  859	SUPT16H			11.35	9.60
  860	PNRC2			13.93	11.64
  861	SRPR			11.87	13.89
  862	BPTF			12.46	11.34
  863	RBM16			12.05	10.52
  864	YWHAZ			13.62	12.61
  865	EDEM3			11.01	12.76
  866	C12orf23			11.18	12.40
  867	DOC2A			3.96	7.21
  868	SETD2			11.54	10.39
  869	FAM98A			10.91	12.10
  870	C13orf18			13.25	9.86
  871	BRP44L			10.48	11.91
  872	CCDC6			11.25	9.82
  873	GLCCI1			12.22	15.19
  874	PLEKHH1			7.03	9.25
  875	SMEK1			11.38	10.37
  876	ANKRD9			4.70	8.61
  877	CSNK1E			8.86	11.48
  878	DCUN1D3			7.48	5.95
  879	LTB			14.66	9.91
  880	EOMES			7.30	6.14
  881	PARP8			10.81	8.69
  882	ABCD1			7.12	8.49
  883	HOXA10			5.56	7.32
  884	RNPS1			12.60	11.25
  885	AYTL2			12.08	10.79
  886	FLJ20699			5.74	8.80
  887	SAPS1			11.43	9.76
  888	SYNJ1			8.36	9.64
  889	ZNF403			12.06	10.91
  890	KPNB1			13.37	12.26
  891	ISOC1			9.67	10.98
  892	HYOU1			9.95	11.85
  893	ZNF706			12.83	14.30
  894	HDLBP			10.60	13.37
  895	ELF1			12.70	11.34
  896	TM9SF4			8.66	10.55
  897	RAB8A			11.94	10.88
  898	MORF4L2			12.94	14.69
  899	SERP1			13.31	15.16
  900	ADAMTS5			5.71	7.31
  901	TRIOBP			11.23	10.18
  902	ZNF664			11.32	12.57
  903	RAB11FIP1			11.78	9.03
  904	MYO18A			8.06	6.19
  905	DUSP10			10.13	8.70
  906	ATXN1			6.66	10.65	6.66	10.25
  907	EPB41L4B			6.12	7.95
  908	CNTNAP1			6.51	9.06
  909	SLC17A7			6.47	8.01
  910	SPCS2			13.75	15.53
  911	CCNC			12.68	14.10
  912	WDR77			10.67	8.83
  913	GMFB			12.03	10.70
  914	ITGA6			6.72	10.51
  915	CASP8AP2			10.55	9.44
  916	SEC24D			8.79	11.88
  917	CBX1			12.16	10.96
  918	TBX3			6.05	7.89
  919	NFAT5			12.09	10.72
  920	ATP6V1B2			10.73	9.53
  921	ACTG1			16.72	14.81
  922	RND3			7.31	5.65
  923	HNRPUL1			11.97	10.58
  924	TJP1			7.18	10.59
  925	RAPGEF4			5.25	8.09
  926	RGMA			5.65	7.42
  927	NAP1L1			14.70	12.60
  928	TARDBP			12.72	11.49
  929	DAZAP1			12.74	11.30
  930	ZNF609			8.26	6.94	8.26	9.52
  931	ARL1			10.44	12.41
  932	ERC2			7.03	5.97
  933	PDE4A			7.65	9.22
  934	CSK			12.83	10.59
  935	IMPAD1			8.44	10.05
  936	PNN			13.75	12.48
  937	DCP2			13.06	10.74
  938	PIK3R3			6.34	7.82
  939	RAB1A			13.59	14.83
  940	CENTB2			12.39	10.97
  941	SPTAN1			11.48	9.79
  942	DNAJC8			12.25	11.00
  943	UNC13B			6.70	8.35
  944	DHX15			14.19	12.99
  945	VEZF1			11.45	9.94
  946	DHDDS			8.74	10.05
  947	FAM55C			8.19	9.74
  948	USP37			8.89	7.60
  949	MMD			11.68	10.32
  950	HCLS1			14.42	13.12
  951	KIAA1370			13.30	14.72
  952	GANC			8.31	6.12
  953	SSRP1			11.34	10.27
  954	G3BP1			12.24	10.55
  955	BAAT			4.91	7.24
  956	FNDC3B			8.27	14.34
  957	FBXW7			10.21	12.19
  958	SPECC1L			10.27	7.66
  959	YBX1			15.31	14.20
  960	YAP1			7.23	5.61
  961	ARF4			12.09	14.23
  962	PNKD			10.86	9.79
  963	CLEC2D			11.96	10.90
  964	XPO1			13.81	11.82
  965	TRAM2			9.38	12.80
  966	MITF					8.05	6.41
  967	CFL1			16.37	14.09
  968	CREB5			5.46	7.24
  969	GREM1			4.48	7.14
  970	LONRF1			11.26	10.00
  971	NME7			9.37	8.30
  972	PMAIP1			12.26	10.75
  973	KLHL14			11.89	10.84
  974	AOF1			9.33	7.96	9.33	8.28
  975	SMPD3			5.90	7.83
  976	RGL1			6.66	8.04
  977	LYPD6			5.38	7.39
  978	STAT5B			10.95	9.73
  979	C10orf6			10.04	8.44
  980	CLDND1			10.72	12.01
  981	DUSP9			5.59	7.70
  982	SLC36A1			8.53	7.23
  983	PAM			8.90	10.86
  984	GHR			5.87	7.56
  985	CBL			11.69	10.26
  986	CENTG2			5.96	7.39
  987	BACH2			13.68	9.35	13.68	11.88
  988	NUP153			12.33	10.55
  989	CPD			8.37	9.72
  990	APLN			8.58	6.47
  991	RFXDC2			11.67	10.00
  992	GABRB2			5.58	8.10
  993	NOMO3			5.39	7.39
  994	RHOJ			6.41	7.75
  995	PRDM1			9.15	14.10
  996	IGSF3			7.62	5.91
  997	UBE2A			12.47	11.47
  998	KBTBD8			12.82	9.73	12.82	11.71
  999	PRR15			7.27	5.94	7.27	4.67
  1000	ILF3			11.94	10.71
  1001	WIPF1			13.81	12.41
  1002	GCNT2			11.01	8.49
  1003	DBNL			10.43	8.48
  1004	C14orf43			10.14	8.01
  1005	MID1IP1			9.16	8.11
  1006	LIMD2			10.43	8.88
  1007	HNRPK			14.49	12.95	14.49	13.46
  1008	ZNF697			5.74	8.07
  1009	TSHZ3			7.31	5.32
  1010	MBNL2			11.88	13.47
  1011	ELL			7.83	9.17
  1012	MAFB			7.30	8.56
  1013	GGA2			13.25	10.77
  1014	C20orf121			10.01	8.93
  1015	CDC2L6			11.52	9.69
  1016	TOB2			11.39	9.58
  1017	MAP4K4			12.04	10.41
  1018	FAM102A			11.41	10.18
  1019	ITGB3BP			11.86	10.76
  1020	HIVEP1			10.35	9.05
  1021	NUDT21			12.51	11.11
  1022	EIF4A1					14.02	12.98
  1023	TSPAN14			8.38	6.31
  1024	SLC2A4RG			8.98	7.40
  1025	DGKZ			9.74	7.85
  1026	PTPN9			9.30	7.85
  1027	BCL7A			11.33	10.10
  1028	LSM14A			12.74	11.19
  1029	GNB2			11.50	10.12
  1030	SLC6A6			11.35	8.51
  1031	TACC1			13.21	11.62
  1032	LIMK2			9.80	8.12
  1033	ACVR2B			6.69	8.33
  1034	SCC-112			12.25	10.55
  1035	PTBP1			13.38	12.19	13.38	12.21
  1036	CITED2			9.88	12.56
  1037	SNX5			14.17	13.16
  1038	ACTR2			15.12	13.32
  1039	OSBPL3			10.59	12.84
  1040	SFRS1			14.60	13.24
  1041	ADM			7.24	11.10
  1042	PFKFB3			11.91	10.01
  1043	FNBP1			13.61	12.25
  1044	MTMR2			10.53	9.06
  1045	DDHD1					10.18	8.74
  1046	HSPA5			13.70	16.32
  1047	PTK2			12.12	8.33
  1048	UBE2I			13.17	11.22
  1049	SNAP23			13.78	12.23
  1050	MTERFD2			10.53	8.59
  1051	COTL1			13.89	10.14
  1052	PHACTR2			8.65	6.86
  1053	GLRA2			5.93	7.69
  1054	NUTF2			10.39	9.26
  1055	TSGA14			9.52	8.03
  1056	FKBP1A			12.16	10.89	12.16	10.82
  1057	LRRC1			8.82	7.68
  1058	PDIA6			13.07	14.91
  1059	YWHAQ			14.50	12.99
  1060	ACHE			5.59	8.29
  1061	KRAS			12.36	10.49
  1062	FAM107B			13.87	12.55
  1063	CCDC117					11.03	9.56
  1064	WDR1			13.38	11.36
  1065	HMGB1			16.36	14.98	16.36	14.97
  1066	CCND3			12.69	9.79
  1067	SUV39H1					9.28	8.07
  1068	RANBP5					11.94	10.87
  1069	SLC25A33			10.24	9.00
  1070	EDNRA			5.94	7.12
  1071	RAB11A			12.94	11.73
  1072	DDEF1			11.98	10.54
  1073	CXorf15			11.84	10.47
  1074	C4orf34			13.65	10.16
  1075	SLC44A1			11.10	13.50
  1076	EIF2AK3			12.29	13.83
  1077	RAP1B			14.21	13.21
  1078	CDK2			10.20	8.73	10.20	9.01
  1079	JDP2			7.94	5.97
  1080	GSTCD			8.86	7.85
  1081	TNKS2			10.95	9.62	10.95	9.62
  1082	FUZ			7.15	8.96	7.15	8.19
  1083	SYPL1			13.56	10.87
  1084	EIF4H					13.07	12.02
  1085	PIP5K2A			9.65	8.06
  1086	RGS2			11.61	14.48
  1087	ARF5					10.35	9.13
  1088	JAKMIP2			8.57	6.96
  1089	RABGAP1			12.19	9.65	12.19	10.85
  1090	LRIG1	9.76	10.77			10.77	9.16
  1091	CLTA			13.08	11.71
  1092	APBB2					7.87	6.39
  1093	SMC1A			12.88	11.33
  1094	CDC27	9.86	10.88
  1095	STT3A	8.64	9.66
  1096	ZFAND6	12.01	13.04	13.04	11.95
  1097	BZRAP1			8.43	7.15
  1098	DPP3	9.41	10.44	10.44	8.39
  1099	CNIH	11.94	12.98
  1100	TMEM16F	9.42	10.46
  1101	ARHGEF7	11.99	13.03	13.03	11.18	13.03	11.60
  1102	CAMTA1	10.60	11.64
  1103	PFN1	14.03	15.08
  1104	USP12	10.18	11.23
  1105	AZIN1	11.24	12.29
  1106	GNA13	13.89	14.94	14.94	13.37
  1107	RCOR1	10.46	11.51	11.51	9.96
  1108	USP1			13.36	11.52
  1109	VANGL1			7.47	9.15
  1110	CAPZB	11.85	12.91
  1111	CDV3	13.86	14.91
  1112	ABI1	11.61	12.67	12.67	10.96
  1113	ST5	7.21	8.28
  1114	TTL	8.63	9.69	9.69	8.03
  1115	ANP32E	12.76	13.82	13.82	12.19
  1116	USP7	12.27	13.34
  1117	BCL11B	7.20	8.27			8.27	9.68
  1118	OAZ2	8.98	10.06
  1119	PPP2CA	12.04	13.12			13.12	11.89
  1120	TSC1	10.58	11.66
  1121	CTDSPL2	9.81	10.90	10.90	9.51	10.90	9.68
  1122	NEDD4L	7.81	8.90
  1123	NAT13	12.06	13.16
  1124	ASF1A	9.54	10.64
  1125	DPF1			7.18	8.50	7.18	5.84
  1126	RAN	13.46	14.57	14.57	12.39
  1127	ELAVL1	11.66	12.77	12.77	11.43
  1128	GRHL1			5.76	7.24
  1129	SMAD2	10.45	11.57
  1130	IQWD1	9.70	10.83			10.83	9.73
  1131	ETS2			7.50	5.66
  1132	CDC25B	10.04	11.18
  1133	USP32	10.65	11.78			11.78	10.75
  1134	EED	11.76	12.90	12.90	11.50
  1135	CTNND2			5.93	7.81
  1136	SHROOM3	7.86	9.01			9.01	6.05
  1137	PSCD3	6.43	7.57
  1138	ATF7	7.83	8.97
  1139	CDK6	10.31	11.46
  1140	GCH1			12.59	10.17
  1141	AP3S1	11.95	13.12
  1142	ARL6IP1	12.03	13.20	13.20	11.51
  1143	TBC1D1	11.00	12.17			12.17	10.01
  1144	ATP2A2	10.75	11.92			11.92	10.76
  1145	SORL1	12.49	13.67	13.67	10.58
  1146	SOX5	9.30	10.47	10.47	7.33	10.47	9.08
  1147	KIAA1411	8.65	9.83
  1148	KIAA0922	12.43	13.61	13.61	10.86	13.61	12.35
  1149	SSX2IP	7.82	9.00			9.00	7.49
  1150	SNRPD1	11.85	13.04
  1151	EDD1	11.23	12.42
  1152	BLMH	9.01	10.21
  1153	PTPLB	11.32	12.52
  1154	SLC25A27					7.44	3.28
  1155	PGAM1	12.40	13.61	13.61	12.55
  1156	HMGA1	11.26	12.47	12.47	10.36
  1157	EDEM1	11.34	12.55
  1158	PRPF38A	10.27	11.48	11.48	10.00
  1159	DCUN1D1	9.82	11.03
  1160	ROBO1	7.43	8.64
  1161	LRRC59			11.26	12.65
  1162	ZNF207	12.29	13.51
  1163	GTDC1	9.19	10.42	10.42	9.35
  1164	C1orf121	10.47	11.70
  1165	NF1	9.23	10.46
  1166	CUL3	12.98	14.21	14.21	12.80	14.21	12.99
  1167	B4GALT5	8.74	9.97	9.97	8.08	9.97	8.71
  1168	GADD45A	8.14	9.37	9.37	11.74
  1169	RASD2			5.53	7.35
  1170	CD4	6.76	8.01	8.01	6.11
  1171	WWC1			6.16	7.88
  1172	DUSP2	9.43	10.69	10.69	9.18
  1173	TP53INP2	8.44	9.70	9.70	8.53	9.70	8.00
  1174	NRAS	8.89	10.16
  1175	TFRC	12.12	13.39	13.39	11.88
  1176	MASTL	8.76	10.03	10.03	8.23	10.03	8.64
  1177	USP6	9.12	10.39			10.39	9.25
  1178	VGLL4	10.30	11.57	11.57	10.16
  1179	C10orf78	8.93	10.21
  1180	BTG3	9.87	11.16
  1181	TMOD2	8.64	9.93	9.93	6.94
  1182	HOXA5	7.03	8.32
  1183	AK2	10.33	11.62
  1184	MAP2K1	10.41	11.71	11.71	10.69	11.71	10.61
  1185	CASP3	10.16	11.47
  1186	STK40	9.30	10.60	10.60	8.13
  1187	GRHL3			5.20	7.46
  1188	SLC25A4	8.65	9.96
  1189	KLHDC5	9.52	10.83
  1190	SAMHD1	9.72	11.03
  1191	PHLPP	7.93	9.24			9.24	7.58
  1192	CCNE1	7.86	9.18
  1193	EGR1	11.92	13.24
  1194	PIM1	9.44	10.76			10.76	8.87
  1195	HECW1	6.49	7.81
  1196	CHAC1			6.73	8.76
  1197	P4HA2	6.84	8.18
  1198	PPM1E	6.59	7.92
  1199	KIAA0746	13.65	14.98
  1200	LOC401720			6.33	7.63
  1201	CAPN5	5.90	7.24
  1202	DYNLL1	12.69	14.03
  1203	EHMT1	10.40	11.75
  1204	RIC8B	7.54	8.89
  1205	GRIN1			7.40	8.93
  1206	DEK	12.73	14.09	14.09	10.29
  1207	E2F1	7.01	8.37			8.37	7.21
  1208	FAM45A	10.66	12.02
  1209	VAMP1	11.10	12.47
  1210	LTBP1	7.23	8.61
  1211	SOCS1	9.18	10.56	10.56	9.25
  1212	ZCCHC14	6.98	8.36
  1213	KLHL3	8.04	9.43
  1214	RET	5.90	7.29	7.29	5.99
  1215	CUTL1	10.33	11.72	11.72	10.67	11.72	10.50
  1216	RBL1	7.58	8.98	8.98	7.52	8.98	7.97
  1217	TOP1	11.47	12.87
  1218	GPD1L	7.94	9.35
  1219	SAR1B	9.03	10.46	10.46	12.55
  1220	MTF2	11.84	13.27	13.27	11.18
  1221	ANP32B			15.53	14.21
  1222	CIT	8.19	9.63	9.63	7.43	9.63	7.77
  1223	POU3F1	6.08	7.52
  1224	MTMR12	11.44	12.89	12.89	11.13	12.89	11.60
  1225	MBOAT2	6.73	8.17			8.17	6.25
  1226	DOCK9	9.28	10.73
  1227	ZAK	8.60	10.05	10.05	8.21
  1228	LOC152485	11.05	12.51	12.51	10.56
  1229	HNRPA3			14.33	12.26
  1230	LMNB1			10.63	8.78
  1231	ZFYVE21	8.42	9.90
  1232	TXNDC5			12.47	16.40
  1233	UBE2G1	11.36	12.84
  1234	KIF23	7.44	8.93	8.93	5.57	8.93	6.64
  1235	DPYSL2	9.98	11.46	11.46	9.03
  1236	ATP5G3	12.15	13.64
  1237	GLRX5	10.08	11.57
  1238	NLK	10.41	11.92
  1239	ARL6IP6	10.51	12.02	12.02	10.43
  1240	CNNM4	6.17	7.68	7.68	5.21
  1241	TBC1D4	9.12	10.64	10.64	8.94
  1242	CD163			5.49	7.00
  1243	PKD2	8.17	9.71			9.71	7.48
  1244	DIAPH3	8.23	9.77	9.77	8.34
  1245	RAD23B	10.65	12.20			12.20	10.54
  1246	DCAMKL2	5.53	7.08	7.08	5.88
  1247	LMBR1	7.22	8.77
  1248	RRAS2	11.24	12.79	12.79	11.03
  1249	MYO1D	6.31	7.88	7.88	10.07
  1250	KLHL5	10.57	12.15	12.15	10.43	12.15	10.80
  1251	EPS15	12.32	13.91			13.91	12.70
  1252	FASLG	5.47	7.06
  1253	H2AFY	12.56	14.16
  1254	LIMA1	8.47	10.07	10.07	9.06
  1255	CDCA4	8.92	10.52	10.52	9.16
  1256	HAS3	5.70	7.31	7.31	6.15
  1257	HRBL	5.96	7.57	7.57	6.35	7.57	5.97
  1258	SYAP1	9.71	11.32			11.32	9.76
  1259	MDFIC	12.06	13.67
  1260	FAM76B	11.27	12.89	12.89	10.52
  1261	SNTB2	8.22	9.85	9.85	8.64
  1262	ARL3	8.92	10.55
  1263	GPR124	5.50	7.14
  1264	BCL2L11	10.79	12.43			12.43	11.14
  1265	RNF103			12.03	13.23
  1266	MYB	6.97	8.62	8.62	5.61
  1267	PKM2	11.21	12.87
  1268	VCL	8.92	10.57	10.57	8.25
  1269	RBBP7	12.45	14.11	14.11	12.96	14.11	12.70
  1270	LBR	12.86	14.53	14.53	12.16	14.53	12.50
  1271	RRBP1	6.97	8.64	8.64	11.94
  1272	GABRB3	5.33	7.00
  1273	SGCB	7.78	9.45	9.45	8.22
  1274	FAM81A	6.61	8.30	8.30	5.44
  1275	RAB15	5.75	7.44
  1276	SOX9	6.72	8.42
  1277	SAP30	8.22	9.91
  1278	BRWD1	8.60	10.30	10.30	8.72	10.30	9.04
  1279	KCNMA1	7.47	9.17	9.17	10.42
  1280	WHSC1	9.67	11.37	11.37	9.60
  1281	CCDC126	9.36	11.09
  1282	GRAMD1C	7.83	9.57
  1283	PHF19	8.98	10.73	10.73	9.18
  1284	ADAM23	7.02	8.77	8.77	7.00
  1285	C9orf150	5.38	7.13
  1286	ZNF572	6.52	8.30	8.30	6.82
  1287	STK39	9.38	11.15	11.15	9.40
  1288	SMS	9.73	11.54
  1289	DMD	8.96	10.77	10.77	9.32
  1290	C1orf83	6.15	7.96
  1291	MFHAS1	9.80	11.61	11.61	9.36
  1292	STXBP1			6.57	7.79
  1293	CPNE2	5.44	7.27	7.27	4.84
  1294	MYH10	7.07	8.90			8.90	7.85
  1295	CALM3	9.63	11.46	11.46	10.30
  1296	EFNB2	7.01	8.85
  1297	ACTN1	5.89	7.74
  1298	RBMS3	5.67	7.51
  1299	ACOT7			9.70	7.83
  1300	RKHD1			5.92	7.77
  1301	LRRK1	9.74	11.62			11.62	10.12
  1302	PTCH1	7.70	9.58
  1303	MGLL	7.30	9.18	9.18	10.57	9.18	8.06
  1304	YWHAH	8.94	10.82	10.82	9.09
  1305	PDE4D	6.78	8.66
  1306	MAF	8.05	9.95	9.95	8.25
  1307	PTGER3	5.18	7.09			7.09	6.06
  1308	PRKCD	9.49	11.40
  1309	CCDC64	8.15	10.07	10.07	7.55
  1310	RASL11A			7.89	6.65
  1311	KPNA2	11.18	13.09	13.09	11.78	13.09	11.55
  1312	GPR137B	8.65	10.58			10.58	9.36
  1313	TIAM1	6.97	8.90
  1314	TFDP1	10.95	12.90	12.90	10.49
  1315	SSBP2	10.47	12.42	12.42	10.32
  1316	REEP1	5.64	7.61
  1317	MAP2	7.85	9.83			9.83	7.75
  1318	HOXA9	5.15	7.12
  1319	SCRN1			11.81	9.54
  1320	LOC129607	8.79	10.78	10.78	9.33	10.78	9.45
  1321	SIAH2	10.35	12.34	12.34	10.57
  1322	DKFZP564O0823	6.96	8.95
  1323	POLQ	8.30	10.30	10.30	8.98	10.30	7.83
  1324	KLF15	5.88	7.88	7.88	9.36	7.88	6.74
  1325	PXDN	8.36	10.37
  1326	BTBD12	8.06	10.08	10.08	8.56
  1327	PHF6	11.19	13.21	13.21	11.96	13.21	12.09
  1328	SLC41A2	5.67	7.68
  1329	HN1	9.87	11.92	11.92	9.85	11.92	10.58
  1330	ZNF608	8.98	11.07	11.07	9.74
  1331	RNGTT	10.32	12.45
  1332	RAP2A	10.35	12.47
  1333	LIMK1	5.18	7.36			7.36	5.05
  1334	SMAD1	5.91	8.10	8.10	6.34
  1335	NCOA7	10.39	12.58
  1336	PRDM15	10.29	12.49
  1337	PELI1	10.67	12.88
  1338	PLS1	5.43	7.65			7.65	4.53
  1339	RAB23	6.14	8.36
  1340	NAP1L5	7.40	9.63	9.63	8.30	9.63	8.34
  1341	DNER	6.90	9.14
  1342	LRRC42	7.58	9.83
  1343	ID2	7.74	9.99
  1344	IBRDC2	9.98	12.24	12.24	8.84	12.24	9.94
  1345	DNMT1	11.21	13.50
  1346	STAC3	5.74	8.02	8.02	7.01
  1347	HMGB3	8.20	10.50	10.50	8.80	10.50	8.08
  1348	BMPR1A	6.75	9.06
  1349	SGK	10.06	12.37
  1350	CBX2	6.06	8.37	8.37	6.38
  1351	LRRC20	5.69	8.03
  1352	LRRC4	4.81	7.15	7.15	5.05
  1353	HOXA1	5.36	7.70
  1354	LRRC62	4.95	7.30
  1355	ATAD2	9.00	11.34	11.34	8.44	11.34	9.37
  1356	MOBKL1A	9.73	12.08	12.08	9.13	12.08	10.12
  1357	LOC220594	10.06	12.41			12.41	10.07
  1358	ZNF804A	6.14	8.51
  1359	C1orf113	5.99	8.36			8.36	5.74
  1360	FMNL2	6.58	8.96
  1361	H2AFX			12.49	10.60
  1362	ATP1B1	5.60	8.03
  1363	GPT2	6.54	8.97
  1364	PSRC1			9.89	7.46
  1365	SLC25A35	5.23	7.68
  1366	LHFPL2	8.94	11.41
  1367	UBE2J1	11.34	13.81			13.81	12.22
  1368	TBC1D8B	5.43	7.91	7.91	6.89
  1369	SGPP1	10.96	13.46	13.46	11.28
  1370	C11orf9	4.74	7.24
  1371	BCL6	12.29	14.82	14.82	10.60	14.82	11.78
  1372	ANUBL1	8.12	10.66			10.66	8.78
  1373	MTA3	8.37	10.92
  1374	PGBD5	4.87	7.45			7.45	5.87
  1375	LPP	11.00	13.59	13.59	10.07	13.59	10.89
  1376	NDFIP2	7.39	9.99
  1377	STMN1			11.76	9.03
  1378	PITPNC1	8.77	11.42	11.42	10.00
  1379	SH3RF1	7.37	10.05	10.05	8.10
  1380	ASF1B	8.56	11.25	11.25	7.91	11.25	8.27
  1381	FLJ20186	12.06	14.82	14.82	12.05
  1382	SLC16A2			8.08	5.17
  1383	PEX5	9.12	11.90	11.90	9.50
  1384	ECT2	7.45	10.23	10.23	7.70	10.23	7.63
  1385	MAML3	8.05	10.85
  1386	TEAD1	4.42	7.24
  1387	HMGB2	12.96	15.78	15.78	12.73	15.78	13.36
  1388	NCALD	5.15	7.97
  1389	RGC32	7.75	10.62
  1390	PPP1R3C	4.18	7.06
  1391	DEPDC1B			9.96	7.11
  1392	WEE1	10.11	13.01	13.01	10.32	13.01	11.84
  1393	FHL2	4.82	7.77
  1394	ITGB8	4.70	7.66
  1395	SLC1A1			10.86	8.88
  1396	FAM83D	7.36	10.32
  1397	UHRF1			12.22	8.72
  1398	C7orf41	4.94	7.95	7.95	6.29
  1399	ZBTB8	5.58	8.59	8.59	11.07
  1400	ZNF367	7.97	10.99	10.99	8.04	10.99	8.22
  1401	CDC25A	6.03	9.09	9.09	6.81
  1402	CHEK1	7.59	10.68	10.68	7.72
  1403	CDCA7	10.61	13.76	13.76	10.16	13.76	11.06
  1404	FGF13	4.55	7.69
  1405	SSBP3	6.20	9.35
  1406	EZH2	9.40	12.57	12.57	10.22
  1407	TNFSF11	5.18	8.36	8.36	5.91	8.36	9.78
  1408	PACSIN1	5.16	8.35
  1409	RRM2	10.59	13.82	13.82	9.47	13.82	10.79
  1410	POU4F1	5.62	8.85
  1411	MYBL2	9.07	12.41	12.41	9.12
  1412	KIAA1212	8.46	11.81			11.81	8.55
  1413	CCNE2	7.30	10.66	10.66	8.02	10.66	8.03
  1414	IGF2BP3	7.92	11.34	11.34	9.93	11.34	9.55
  1415	PRKAR2B	6.17	9.66	9.66	8.16
  1416	CADPS	5.04	8.58	8.58	6.18
  1417	ANKRD15	6.97	10.52			10.52	7.58
  1418	DAAM1	9.33	12.92
  1419	MAP1B	4.05	7.68	7.68	5.03	7.68	6.23
  1420	AFF2	6.32	10.00
  1421	E2F7	5.66	9.37	9.37	6.53	9.37	6.89
  1422	PRC1	8.37	12.08
  1423	ENPP5	3.54	7.26	7.26	4.97
  1424	E2F8	7.68	11.41
  1425	MED12L	7.82	11.59	11.59	7.46	11.59	10.57
  1426	LOC162073	6.30	10.08
  1427	LRRC32	4.14	7.93	7.93	6.61	7.93	4.95
  1428	DMXL2	6.57	10.38	10.38	6.61	10.38	7.76
  1429	FLJ20366	7.86	11.68	11.68	7.62	11.68	9.86
  1430	TOX	9.05	12.88	12.88	11.27
  1431	MME			11.56	10.33
  1432	FGD6	7.63	11.73	11.73	9.12
  1433	MYBL1	9.68	14.13	14.13	10.02	14.13	9.72
  *Empty cells indicate values not appreciably measured.

• TABLE 16
  Predictor microRNAs that distinguish activated B-cell (ABC)
  DLBCL from germinal center B-cell (GCB) DLBC

  		Higher
  	ABC vs GCB	in
  	hsa-miR-93/mmu-miR-93/rno-miR-93	GCB
  	hsa-miR-331-3p/mmu-miR-331-3p/rno-miR-331	GCB
  	hsa-miR-129*	GCB
  	hsa-miR-423-3p/mmu-miR-423-3p/rno-miR-423	GCB
  	hsa-miR-28-5p/mmu-miR-28/rno-miR-28	GCB
  	mghv-miR-M1-7-3p	GCB
  	hsa-miR-518b	GCB
  	ebv-miR-BHRF1-1	GCB
  	hsa-miR-140-5p/mmu-miR-140/rno-miR-140	GCB
  	hsa-miR-505*	GCB
  	hsa-miR-675	GCB
  	hsa-miR-198	GCB
  	hsa-miR-125b-1*/mmu-miR-125b-3p/mo-miR-	GCB
  	125b-3p
  	hsa-miR-361-5p/mmu-miR-361/rno-miR-361	GCB
  	ebv-miR-BART8*	GCB

• TABLE 17
  Predictor microRNAs that distinguish activated B-cell (ABC)
  DLBCL from Burkitt lymphoma

  		Higher
  	ABC vs BL	in
  	hsa-miR-155	ABC
  	hsa-miR-29c/mmu-miR-	ABC
  	29c/rno-miR-29c
  	hsa-miR-146b-5p/mmu-miR-	ABC
  	146b/rno-miR-146b
  	hsa-miR-29b/mmu-miR-	ABC
  	29b/rno-miR-29b
  	hsa-miR-22/mmu-miR-	ABC
  	22/rno-miR-22
  	hsa-miR-21/mmu-miR-	ABC
  	21/rno-miR-21
  	hsa-miR-768-3p	ABC
  	hsa-miR-145/mmu-miR-	ABC
  	145/rno-miR-145
  	hsa-miR-29a/mmu-miR-	ABC
  	29a/rno-miR-29a
  	hsa-miR-30e/mmu-miR-	ABC
  	30e/rno-miR-30e
  	hsa-miR-26a/mmu-miR-	ABC
  	26a/rno-miR-26a
  	hsa-miR-101/mmu-miR-	ABC
  	101a/rno-miR-101a
  	hsa-miR-24/mmu-miR-	ABC
  	24/rno-miR-24
  	hsa-miR-26b/mmu-miR-	ABC
  	26b/rno-miR-26b
  	hsa-miR-27a/mmu-miR-	ABC
  	27a/rno-miR-27a
  	hsa-miR-27b/mmu-miR-	ABC
  	27b/rno-miR-27b
  	hsa-miR-23b/mmu-miR-	ABC
  	23b/rno-miR-23b
  	hsa-miR-23a/mmu-miR-	ABC
  	23a/rno-miR-23a
  	hsa-miR-125b/mmu-miR-	ABC
  	125b-5p/rno-miR-125b-5p
  	hsa-miR-30a/mmu-miR-	ABC
  	30a/rno-miR-30a
  	hsa-miR-142-3p/mmu-miR-	ABC
  	142-3p/rno-miR-142-3p
  	hsa-let-7a/mmu-let-7a/rno-let-	ABC
  	7a
  	hsa-miR-30b/mmu-miR-	ABC
  	30b/rno-miR-30b-5p
  	hsa-miR-142-5p/mmu-miR-	ABC
  	142-5p/rno-miR-142-5p
  	hsa-miR-34b/mmu-miR-34b-3p	ABC
  	hsa-miR-16/mmu-miR-16/rno-	ABC
  	miR-16
  	hsa-miR-30c/mmu-miR-	ABC
  	30c/rno-miR-30c
  	hsa-let-7c/mmu-let-7c/rno-let-	ABC
  	7c
  	hsa-miR-550	ABC
  	hsa-miR-921	BL
  	hsa-miR-30c-2*/mmu-miR-	BL
  	30c-2*/rno-miR-30c-2*
  	hsa-miR-933	BL
  	hsa-miR-658	BL
  	hsa-miR-628-3p	BL
  	hsa-miR-503	BL
  	hsa-miR-193a-5p	BL
  	hsa-miR-30b*	BL
  	hsa-miR-93/mmu-miR-93/rno-	BL
  	miR-93
  	hsa-miR-18b	BL
  	hsa-miR-18a/mmu-miR-	BL
  	18a/rno-miR-18a
  	hsa-miR-874/mmu-miR-	BL
  	874/rno-miR-874

• TABLE 18
  Predictor microRNAs that distinguish activated B-cell (ABC)
  DLBCL from chronic lymphocytic leukemia

  		Higher
  	ABC vs CLL	in
  	hsa-miR-125b/mmu-miR-	ABC
  	125b-5p/rno-miR-125b-5p
  	hsa-miR-126/mmu-miR-	ABC
  	126-3p/rno-miR-126
  	hsa-miR-199a-3p/hsa-	ABC
  	miR-199b-3p/mmu-miR-
  	199a-3p/mmu-miR-
  	199b/rno-miR-199a-3p
  	hsa-miR-145/mmu-miR-	ABC
  	145/rno-miR-145
  	hsa-miR-143/mmu-miR-	ABC
  	143/rno-miR-143
  	hsa-miR-637	ABC
  	hsa-miR-371-5p	ABC
  	kshv-miR-K12-6-3p	ABC
  	hsa-miR-628-3p	ABC
  	hsa-miR-126*/mmu-miR-	ABC
  	126-5p/rno-miR-126*
  	hsa-miR-193a-5p	ABC
  	hsa-miR-21/mmu-miR-	ABC
  	21/rno-miR-21
  	hsa-miR-24/mmu-miR-	ABC
  	24/rno-miR-24
  	hsa-miR-503	ABC
  	hsa-miR-23a/mmu-miR-	ABC
  	23a/rno-miR-23a
  	hsa-miR-23b/mmu-miR-	ABC
  	23b/rno-miR-23b
  	hsa-miR-22/mmu-miR-	ABC
  	22/rno-miR-22
  	hsa-miR-665	ABC
  	hsa-let-7c/mmu-let-7c/rno-	ABC
  	let-7c
  	hsa-miR-658	ABC
  	hsa-let-7a/mmu-let-7a/rno-	ABC
  	let-7a
  	mghv-miR-M1-4	ABC
  	hsa-miR-933	ABC
  	hsa-miR-550	CLL
  	hsa-miR-30e/mmu-miR-	CLL
  	30e/rno-miR-30e
  	hsa-miR-142-	CLL
  	5p/mmu-miR-142-
  	5p/rno-miR-142-5p
  	hsa-miR-101/mmu-	CLL
  	miR-101a/rno-miR-
  	101a
  	hsa-miR-185/mmu-	CLL
  	miR-185/rno-miR-185
  	hsa-miR-888*	CLL
  	hsa-miR-199a-	CLL
  	5p/mmu-miR-199a-
  	5p/rno-miR-199a-5p
  	hsa-miR-668/mmu-	CLL
  	miR-668
  	hsa-miR-549	CLL
  	hsa-miR-801/mmu-	CLL
  	miR-801
  	hsa-miR-649	CLL
  	hsa-miR-625*	CLL
  	hsa-miR-140-	CLL
  	3p/mmu-miR-
  	140*/rno-miR-140*
  	hsa-let-7f/mmu-let-	CLL
  	7f/rno-let-7f
  	hsa-miR-768-5p	CLL
  	hsa-miR-24-1*/mmu-	CLL
  	miR-24-1*/rno-miR-
  	24-1*
  	ebv-miR-BART13	CLL
  	hsa-miR-339-	CLL
  	5p/mmu-miR-339-
  	5p/rno-miR-339-5p
  	hsa-miR-20b/mmu-	CLL
  	miR-20b/rno-miR-
  	20b-5p
  	hsa-miR-335/mmu-	CLL
  	miR-335-5p/rno-miR-
  	335
  	mghv-miR-M1-8	CLL
  	hsa-miR-30d/mmu-	CLL
  	miR-30d/rno-miR-30d
  	hsa-miR-363/mmu-	CLL
  	miR-363/rno-miR-363
  	hsa-miR-361-3p	CLL
  	hsa-miR-107/mmu-	CLL
  	miR-107/rno-miR-107
  	hsa-miR-154/mmu-	CLL
  	miR-154/rno-miR-154
  	hsa-miR-638	CLL
  	hsa-miR-340/mmu-	CLL
  	miR-340-5p/rno-miR-
  	340-5p
  	hsa-miR-331-	CLL
  	3p/mmu-miR-331-
  	3p/rno-miR-331
  	hsa-miR-151-	CLL
  	5p/mmu-miR-151-
  	5p/rno-miR-151
  	hsa-miR-636	CLL
  	hsa-miR-33a/mmu-	CLL
  	miR-33/rno-miR-33
  	hsa-miR-486-	CLL
  	5p/mmu-miR-486
  	hsa-miR-150/mmu-	CLL
  	miR-150/rno-miR-
  	150
  	hsa-miR-25/mmu-	CLL
  	miR-25/rno-miR-25
  	hsa-miR-331-	CLL
  	5p/mmu-miR-331-5p
  	hsa-miR-299-	CLL
  	5p/mmu-miR-
  	299*/rno-miR-299
  	hsa-miR-891a	CLL
  	hsa-miR-144*	CLL
  	hsa-miR-363*/rno-	CLL
  	miR-363*
  	hsa-miR-93/mmu-	CLL
  	miR-93/rno-miR-93
  	hsa-miR-423-	CLL
  	3p/mmu-miR-423-
  	3p/rno-miR-423
  	hsa-let-7g/mmu-let-	CLL
  	7g
  	hsa-miR-28-5p/mmu-	CLL
  	miR-28/rno-miR-28
  	hsa-miR-20b*	CLL
  	hsa-miR-140-	CLL
  	5p/mmu-miR-
  	140/rno-miR-140
  	hsa-miR-519d	CLL
  	hsa-miR-147	CLL
  	hsa-miR-487b/mmu-	CLL
  	miR-487b/rno-miR-
  	487b
  	hsa-miR-361-	CLL
  	5p/mmu-miR-
  	361/rno-miR-361
  	hsa-miR-186/mmu-	CLL
  	miR-186/rno-miR-
  	186
  	hsa-miR-32/mmu-	CLL
  	miR-32/rno-miR-32
  	hsa-miR-129*	CLL
  	hsa-miR-30e*/mmu-	CLL
  	miR-30e*/rno-miR-
  	30e*
  	hsa-miR-196a*/mmu-	CLL
  	miR-196a*/rno-miR-
  	196a*

• TABLE 19
  Predictor microRNAs that distinguish activated B-cell (ABC)
  DLBCL from follicular lymphoma

  		Higher
  	ABC vs FL	in
  	hsa-miR-938	ABC
  	hsa-miR-183*/mmu-miR-	ABC
  	183*
  	hsa-miR-197/mmu-miR-	ABC
  	197
  	hsa-miR-382/mmu-miR-	ABC
  	382/rno-miR-382
  	hsa-miR-20b*	ABC
  	hsa-miR-524-5p	ABC
  	hsa-miR-337-3p	ABC
  	hsa-miR-600	ABC
  	hsa-miR-96/mmu-miR-	ABC
  	96/rno-miR-96
  	mghv-miR-M1-8	ABC
  	hsa-miR-29c*/mmu-miR-	ABC
  	29c*/rno-miR-29c*
  	hsa-miR-575	ABC
  	hsa-miR-518a-3p	ABC
  	hsa-miR-361-5p/mmu-miR-	ABC
  	361/rno-miR-361
  	hsa-miR-193b*	ABC
  	hsa-miR-340*/mmu-miR-	ABC
  	340-3p/rno-miR-340-3p
  	hsa-miR-708/mmu-miR-	ABC
  	708/rno-miR-708
  	hsa-miR-129*	ABC
  	hsa-miR-525-5p	ABC
  	hsa-miR-497/mmu-miR-	FL
  	497/rno-miR-497
  	hsa-miR-22*/mmu-miR-	FL
  	22*/rno-miR-22*
  	hsa-miR-130b/mmu-miR-	FL
  	130b/rno-miR-130b
  	hsa-miR-551b*	FL
  	hsa-miR-331-3p/mmu-miR-	FL
  	331-3p/rno-miR-331
  	ebv-miR-BART13	FL
  	hsa-miR-877/mmu-miR-	FL
  	877/rno-miR-877
  	hsa-miR-636	FL
  	hsa-miR-922	FL
  	hsa-miR-198	FL
  	hsa-miR-342-5p/mmu-miR-	FL
  	342-5p/rno-miR-342-5p
  	hsa-miR-585	FL
  	ebv-miR-BART8*	FL
  	hsa-miR-617	FL
  	hsa-miR-221*	FL
  	hsa-miR-125b-1*/mmu-	FL
  	miR-125b-3p/rno-miR-
  	125b-3p
  	hsa-miR-93/mmu-miR-	FL
  	93/rno-miR-93
  	hsa-miR-363*/rno-miR-	FL
  	363*
  	hsa-miR-744/mmu-miR-	FL
  	744
  	hsa-miR-659	FL
  	hsa-miR-490-3p/mmu-miR-	FL
  	490
  	hsa-let-7d/mmu-let-7d/rno-	FL
  	let-7d
  	hsa-miR-361-3p	FL
  	ebv-miR-BHRF1-1	FL
  	hsa-miR-92b/mmu-miR-	FL
  	92b/rno-miR-92b
  	hsa-miR-151-5p/mmu-miR-	FL
  	151-5p/rno-miR-151
  	hsa-miR-144*	FL
  	hsa-miR-425/mmu-miR-	FL
  	425/rno-miR-425
  	hsa-miR-138/mmu-miR-	FL
  	138/rno-miR-138
  	hsa-miR-92a/mmu-miR-	FL
  	92a/rno-miR-92a
  	hsa-miR-151-3p	FL
  	hsa-miR-25/mmu-miR-	FL
  	25/rno-miR-25
  	hsa-miR-509-3-5p	FL
  	hsa-miR-30e*/mmu-miR-	FL
  	30e*/rno-rniR-30e*
  	hsa-miR-28-5p/mmu-miR-	FL
  	28/rno-miR-28
  	hsa-miR-200b*/mmu-miR-	FL
  	200b*
  	hsa-miR-148b/mmu-miR-	FL
  	148b/rno-miR-148b-3p
  	hsa-miR-488	FL
  	hsa-miR-99b/mmu-miR-	FL
  	99b/rno-miR-99b
  	hsa-miR-339-5p/mmu-miR-	FL
  	339-5p/rno-miR-339-5p
  	hsv1-miR-H1	FL
  	hsa-miR-32/mmu-miR-	FL
  	32/rno-miR-32
  	hsa-miR-885-5p	FL
  	hsa-miR-630	FL
  	ebv-miR-BART16	FL
  	hsa-miR-505*	FL
  	hsa-miR-374b*	FL
  	hsa-miR-574-3p/mmu-miR-	FL
  	574-3p
  	hsa-miR-874/mmu-miR-	FL
  	874/rno-miR-874
  	hsa-miR-423-3p/mmu-miR-	FL
  	423-3p/rno-miR-423
  	hsa-miR-889	FL
  	hcmv-miR-UL148D	FL
  	hsa-miR-487b/mmu-miR-	FL
  	487b/rno-miR-487b
  	hsa-miR-552	FL
  	hsa-miR-220b	FL
  	hsa-miR-551a	FL
  	hsa-let-7d*/mmu-let-	FL
  	7d*/rno-let-7d*
  	kshv-miR-K12-5	FL
  	hsa-miR-629*	FL
  	hsa-miR-99b*/mmu-miR-	FL
  	99b*/rno-miR-99b*
  	hsa-miR-615-3p/mmu-miR-	FL
  	615-3p
  	hsa-miR-657	FL
  	hsa-miR-301a/mmu-miR-	FL
  	301a/rno-miR-301a
  	hsa-miR-518b	FL
  	hsa-miR-194/mmu-	FL
  	miR-194/rno-miR-
  	194
  	hsa-miR-647	FL
  	kshv-miR-K12-6-5p	FL
  	hsa-miR-622	FL
  	hsa-miR-516b	FL
  	hsa-miR-675	FL
  	hsa-miR-526b	FL
  	hsa-miR-671-	FL
  	5p/mmu-miR-671-5p
  	hsa-miR-18a/mmu-	FL
  	miR-18a/rno-miR-
  	18a
  	hsa-miR-18b	FL
  	hsa-miR-181b/mmu-	FL
  	miR-181b/rno-miR-
  	181b
  	hsa-miR-215	FL
  	hsa-miR-153/mmu-	FL
  	miR-153/rno-miR-
  	153
  	hsa-miR-625	FL
  	hsa-miR-510	FL
  	hsa-miR-519d	FL
  	mghv-miR-M1-7-3p	FL
  	hsa-miR-485-	FL
  	3p/mmu-miR-485*
  	hsa-miR-483-5p	FL
  	hsa-miR-140-	FL
  	5p/mmu-miR-
  	140/rno-miR-140
  	hsa-miR-921	FL
  	hsa-miR-186/mmu-	FL
  	miR-186/rno-miR-
  	186
  	hsa-miR-196a*/mmu-	FL
  	miR-196a*/rno-miR-
  	196a*
  	hsa-miR-381/mmu-	FL
  	miR-381/rno-miR-
  	381
  	hsa-miR-620	FL
  	hsa-miR-152/mmu-	FL
  	miR-152/rno-miR-
  	152
  	hsa-miR-766	FL
  	mghv-miR-M1-7-5p	FL
  	hsa-miR-374b/mmu-	FL
  	miR-374/rno-miR-
  	374
  	hsa-let-7c/mmu-let-	FL
  	7c/rno-let-7c
  	kshv-miR-K12-8	FL
  	mghv-miR-M1-3	FL
  	hsa-miR-920	FL
  	hsa-miR-519e*	FL
  	hsa-miR-147	FL
  	hsa-miR-424	FL
  	hsa-miR-193b	FL
  	ebv-miR-BART19-3p	FL
  	hsa-miR-146b-3p	FL
  	hsa-miR-30c/mmu-	FL
  	miR-30c/rno-miR-
  	30c
  	hsa-miR-30a/mmu-	FL
  	miR-30a/rno-miR-
  	30a
  	hsa-miR-939	FL
  	hsa-let-7a/mmu-let-	FL
  	7a/rno-let-7a
  	hsa-miR-122*	FL
  	hsa-miR-206/mmu-	FL
  	miR-206/rno-miR-
  	206
  	ebv-miR-BART18-3p	FL
  	hsa-miR-183/mmu-	FL
  	miR-183/rno-miR-
  	183
  	hsa-miR-9*/mmu-	FL
  	miR-9*/rno-miR-9*
  	kshv-miR-K12-1	FL
  	hsa-miR-34c-	FL
  	5p/mmu-miR-
  	34c/rno-miR-34c
  	hsa-miR-934	FL
  	hsa-miR-890	FL
  	hsa-miR-514	FL
  	hsa-miR-297/mmu-	FL
  	miR-297a
  	hsa-miR-553	FL
  	hsa-miR-765	FL
  	hsa-let-7b*/mmu-let-	FL
  	7b*/rno-let-7b*
  	hsa-miR-500*	FL
  	hsa-miR-601	FL
  	ebv-miR-BHRF1-3	FL
  	hsa-miR-296-	FL
  	3p/mmu-miR-296-
  	3p/rno-miR-296
  	hsa-miR-574-	FL
  	5p/mmu-miR-574-5p
  	hsa-miR-409-	FL
  	5p/mmu-miR-409-
  	5p/rno-miR-409-5p
  	hsa-miR-195*	FL
  	hsa-miR-635	FL
  	hsa-miR-542-	FL
  	3p/mmu-miR-542-
  	3p/rno-miR-542-3p
  	hcmv-miR-US25-1*	FL
  	mghv-miR-M1-2	FL
  	hsa-miR-509-5p	FL
  	hsa-miR-340/mmu-	FL
  	miR-340-5p/rno-
  	miR-340-5p
  	hsa-miR-891a	FL
  	hsa-miR-23a/mmu-	FL
  	miR-23a/rno-miR-
  	23a
  	hsa-miR-19b/mmu-	FL
  	miR-19b/rno-miR-
  	19b
  	hsa-miR-515-5p	FL
  	hsa-miR-23b/mmu-	FL
  	miR-23b/rno-miR-
  	23b
  	hsa-miR-498	FL
  	hsa-miR-886-5p	FL
  	hsa-miR-220c	FL
  	hsa-miR-10a/mmu-	FL
  	miR-10a/rno-miR-
  	10a-5p
  	hsa-miR-32*	FL
  	hsa-miR-24/mmu-	FL
  	miR-24/rno-miR-24
  	hsa-miR-98/mmu-	FL
  	miR-98/rno-miR-98
  	hsa-let-7g/mmu-let-7g	FL
  	hsa-miR-302a/mmu-	FL
  	miR-302a
  	hsa-miR-625*	FL
  	hsa-miR-30b*	FL
  	hsa-miR-30b/mmu-	FL
  	miR-30b/rno-miR-
  	30b-5p
  	hsa-miR-377*	FL
  	hsa-miR-106b*/mmu-	FL
  	miR-106b*/rno-miR-
  	106b*
  	hsa-miR-181a-2*	FL
  	hsa-miR-887	FL
  	hsa-miR-208a/mmu-	FL
  	miR-208a/rno-miR-
  	208
  	ebv-miR-BART7*	FL
  	hsa-miR-7-2*	FL
  	hsa-miR-155*	FL
  	hsa-miR-513a-3p	FL
  	kshv-miR-K12-7	FL
  	hsa-miR-299-3p	FL
  	hsa-miR-218-2*/mmu-	FL
  	miR-218-2*/rno-miR-
  	218*
  	hsa-miR-130b*/mmu-	FL
  	miR-130b*
  	hsa-miR-620	FL
  	hsa-miR-33a/mmu-	FL
  	miR-33/rno-miR-33
  	hsa-miR-7/mmu-miR-	FL
  	7a/rno-miR-7a
  	ebv-miR-BART6-3p	FL
  	hsa-miR-22/mmu-	FL
  	miR-22/rno-miR-22
  	hsa-miR-199b-5p	FL
  	hsa-miR-768-3p	FL
  	hsa-miR-494/mmu-	FL
  	miR-494/rno-miR-494
  	hsa-miR-602	FL
  	hsa-miR-125b-2*/rno-	FL
  	miR-125b*
  	hsa-miR-300	FL
  	hsa-let-7e/mmu-let-	FL
  	7e/rno-let-7e
  	hsa-miR-298	FL
  	hsa-miR-576-3p	FL
  	hsa-miR-187*	FL
  	hsa-miR-365/mmu-	FL
  	miR-365/rno-miR-365
  	hsa-miR-518a-5p/hsa-	FL
  	miR-527
  	hsa-miR-302d*	FL
  	hsa-miR-105	FL
  	hsa-miR-126/mmu-	FL
  	miR-126-3p/rno-miR-
  	126
  	hsa-miR-107/mmu-	FL
  	miR-107/rno-miR-107
  	hsa-miR-299-5p/mmu-	FL
  	miR-299*/rno-miR-
  	299
  	hsa-miR-28-3p/rno-	FL
  	miR-28*
  	hsa-miR-21/mmu-	FL
  	miR-21/rno-miR-21
  	hsa-miR-27b/mmu-	FL
  	miR-27b/rno-miR-27b
  	hsa-miR-516a-5p	FL
  	hsa-miR-129-5p/mmu-	FL
  	miR-129-5p/rno-miR-
  	129
  	hsa-miR-583	FL
  	hsa-miR-483-3p	FL
  	hsa-miR-326/mmu-	FL
  	miR-326/rno-miR-326
  	hsa-miR-548d-5p	FL
  	hsa-miR-629	FL
  	ebv-miR-BART5	FL
  	hsa-miR-665	FL
  	hsa-miR-493	FL
  	hsa-miR-484/mmu-	FL
  	miR-484/rno-miR-484
  	hsa-miR-645	FL
  	hsa-miR-452	FL
  	hsa-miR-518c*	FL
  	hsa-miR-24-2*/mmu-	FL
  	miR-24-2*/rno-miR-
  	24-2*
  	hsa-miR-124/mmu-	FL
  	miR-124/rno-miR-124
  	hsa-miR-184/mmu-	FL
  	miR-184/rno-miR-184
  	hsa-miR-27a*/mmu-	FL
  	miR-27a*/rno-miR-
  	27a*
  	hsa-miR-25*	FL
  	hsa-miR-34b/mmu-	FL
  	miR-34b-3p
  	ebv-miR-BART17-5p	FL
  	hsa-miR-658	FL
  	hsa-miR-212/mmu-	FL
  	miR-212/rno-miR-212
  	hsa-miR-99a/mmu-	FL
  	miR-99a/rno-miR-99a
  	hsa-miR-801/mmu-	FL
  	miR-801
  	hsa-miR-491-3p	FL
  	hsa-miR-551b/mmu-	FL
  	miR-551b/rno-miR-
  	551b
  	hsa-miR-214/mmu-	FL
  	miR-214/rno-miR-214
  	hsa-miR-30e/mmu-	FL
  	miR-30e/rno-miR-30e
  	hsa-miR-888*	FL
  	hsa-miR-505/rno-miR-	FL
  	505
  	hsa-miR-27a/mmu-	FL
  	miR-27a/rno-miR-27a
  	hsa-miR-15a/mmu-	FL
  	miR-15a
  	hsa-miR-20a/mmu-	FL
  	miR-20a/rno-miR-20a
  	kshv-miR-K12-3	FL
  	hsa-miR-455-3p	FL
  	hsa-miR-486-5p/mmu-	FL
  	miR-486

• TABLE 20
  Predictor microRNAs that distinguish activated B-cell (ABC)
  DLBCL from Hodgkin's lymphoma

  		Higher
  	ABC vs HL	in
  	hsa-miR-19b/mmu-miR-	ABC
  	19b/rno-miR-19b
  	hsa-miR-30b/mmu-miR-	ABC
  	30b/rno-miR-30b-5p
  	hsa-miR-142-3p/mmu-	ABC
  	miR-142-3p/rno-miR-142-
  	3p
  	hsa-miR-768-3p	ABC
  	hsa-miR-30c/mmu-miR-	ABC
  	30c/rno-miR-30c
  	hsa-miR-29b/mmu-miR-	ABC
  	29b/rno-miR-29b
  	hsa-miR-24/mmu-miR-	ABC
  	24/rno-miR-24
  	hsa-miR-22/mmu-miR-	ABC
  	22/rno-miR-22
  	hsa-miR-30e/mmu-miR-	ABC
  	30e/rno-miR-30e
  	hsa-miR-30a/mmu-miR-	ABC
  	30a/rno-miR-30a
  	hsa-miR-26b/mmu-miR-	ABC
  	26b/rno-miR-26b
  	hsa-miR-26a/mmu-miR-	ABC
  	26a/rno-miR-26a
  	hsa-miR-15a/mmu-miR-	ABC
  	15a
  	hsa-miR-16/mmu-miR-	ABC
  	16/rno-miR-16
  	hsa-miR-142-5p/mmu-	ABC
  	miR-142-5p/rno-miR-142-
  	5p
  	hsa-miR-29a/mmu-miR-	ABC
  	29a/rno-miR-29a
  	hsa-miR-101/mmu-miR-	ABC
  	101a/rno-miR-101a
  	hsa-miR-126/mmu-miR-	ABC
  	126-3p/rno-miR-126
  	hsa-miR-451/mmu-miR-	ABC
  	451/rno-miR-451
  	hsa-let-7a/mmu-let-7a/rno-	ABC
  	let-7a
  	hsa-miR-23b/mmu-miR-	ABC
  	23b/rno-miR-23b
  	hsa-miR-21/mmu-miR-	ABC
  	21/rno-miR-21
  	hsa-miR-29c/mmu-miR-	ABC
  	29c/rno-miR-29c
  	hsa-miR-20a/mmu-miR-	ABC
  	20a/rno-miR-20a
  	hsa-miR-27b/mmu-miR-	ABC
  	27b/rno-miR-27b
  	hsa-miR-23a/mmu-miR-	ABC
  	23a/rno-miR-23a
  	hsa-miR-27a/mmu-miR-	ABC
  	27a/rno-miR-27a
  	hsa-miR-550	ABC
  	hsa-let-7c/mmu-let-7c/rno-	ABC
  	let-7c
  	hsa-miR-34b/mmu-miR-	ABC
  	34b-3p
  	hsa-miR-933	HL
  	hsa-miR-30c-2*/mmu-	HL
  	miR-30c-2*/rno-miR-30c-
  	2*
  	hsa-miR-503	HL
  	hsa-miR-765	HL
  	hsa-miR-658	HL
  	hsa-miR-620	HL
  	hsa-miR-921	HL
  	hsa-miR-30b*	HL
  	mghv-miR-M1-4	HL
  	hsa-miR-939	HL
  	hsa-miR-494/mmu-miR-	HL
  	494/rno-miR-494
  	hsa-miR-32*	HL
  	hsa-miR-491-3p	HL
  	hsa-miR-10a/mmu-miR-	HL
  	10a/rno-miR-10a-5p
  	hsa-miR-33a/mmu-miR-	HL
  	33/rno-miR-33
  	hsa-miR-99a/mmu-miR-	HL
  	99a/rno-miR-99a
  	hsa-miR-199b-5p	HL
  	hsa-miR-365/mmu-miR-	HL
  	365/rno-miR-365
  	hsa-miR-520d-5p	HL
  	hsa-miR-518c*	HL
  	hsa-miR-32/mmu-miR-	HL
  	32/rno-miR-32
  	hsa-miR-214/mmu-miR-	HL
  	214/rno-miR-214
  	hsa-miR-98/mmu-miR-	HL
  	98/rno-miR-98
  	hsa-miR-302d*	HL
  	hsa-miR-30e*/mmu-miR-	HL
  	30e*/rno-miR-30e*
  	hsa-miR-374b/mmu-miR-	HL
  	374/rno-miR-374
  	hsa-miR-29a*/mmu-miR-	HL
  	29a*/rno-miR-29a*
  	hsa-miR-532-5p/mmu-	HL
  	miR-532-5p/rno-miR-532-
  	5p
  	hsa-miR-149*	HL
  	hsa-miR-422a	HL
  	ebv-miR-BHRF1-2	HL
  	hsa-miR-634	HL
  	hsa-miR-143*	HL
  	hsa-miR-620	HL
  	hsa-miR-660	HL
  	hsa-miR-140-5p/mmu-	HL
  	miR-140/rno-miR-140
  	hsa-miR-28-5p/mmu-miR-	HL
  	28/rno-miR-28
  	hsa-miR-519c-5p/hsa-	HL
  	miR-519b-5p/hsa-miR-
  	523*/hsa-miR-518e*/hsa-
  	miR-522*/hsa-miR-519a*
  	hsa-miR-505/rno-miR-505	HL
  	hsa-miR-184/mmu-miR-	HL
  	184/rno-miR-184
  	hsa-miR-107/mmu-miR-	HL
  	107/rno-miR-107
  	hsa-miR-298	HL
  	hsa-miR-455-3p	HL
  	hsa-miR-638	HL
  	hsa-miR-502-3p	HL
  	hsa-miR-149/mmu-miR-	HL
  	149
  	hsa-miR-583	HL
  	hsa-miR-105	HL
  	hsa-miR-128/mmu-miR-	HL
  	128/rno-miR-128
  	hsa-miR-656	HL
  	hsa-miR-497/mmu-miR-	HL
  	497/rno-miR-497
  	hsa-miR-152/mmu-miR-	HL
  	152/rno-miR-152
  	hsa-miR-151-5p/mmu-	HL
  	miR-151-5p/rno-miR-151
  	hsa-miR-148b/mmu-miR-	HL
  	148b/rno-miR-148b-3p
  	hsa-miR-300	HL
  	hsa-miR-144*	HL
  	hsa-miR-145*/mmu-miR-	HL
  	145*
  	hcmv-miR-UL70-3p	HL
  	hsa-miR-28-3p/rno-miR-	HL
  	28*
  	hsa-miR-27a*/mmu-miR-	HL
  	27a*/rno-miR-27a*
  	hsa-miR-194/mmu-miR-	HL
  	194/rno-miR-194
  	hsa-miR-130b*/mmu-miR-	HL
  	130b*
  	hsa-miR-548d-5p	HL
  	hsa-miR-937	HL
  	hsa-miR-7/mmu-miR-	HL
  	7a/rno-miR-7a
  	hsa-miR-518a-5p/hsa-	HL
  	miR-527
  	hsa-miR-323-3p/mmu-	HL
  	miR-323-3p/rno-miR-323
  	hsa-miR-215	HL
  	hsa-miR-513a-3p	HL
  	hsa-miR-595	HL
  	hsa-miR-515-5p	HL
  	hsa-miR-483-3p	HL
  	hsa-miR-330-5p/mmu-	HL
  	miR-330/rno-miR-330
  	hsa-miR-18b	HL
  	hsa-miR-509-3p	HL
  	hsa-miR-151-3p	HL
  	hsa-miR-934	HL
  	hsa-miR-328/mmu-miR-	HL
  	328/rno-miR-328
  	hsa-miR-187*	HL
  	kshv-miR-K12-3	HL
  	hsa-miR-373*	HL
  	hsa-miR-96/mmu-miR-	HL
  	96/rno-miR-96
  	hsa-miR-186/mmu-miR-	HL
  	186/rno-miR-186
  	hsa-miR-886-5p	HL
  	hsa-miR-424	HL
  	hsa-miR-147	HL
  	hsa-miR-340/mmu-miR-	HL
  	340-5p/rno-miR-340-5p
  	hsa-miR-129-5p/mmu-	HL
  	miR-129-5p/rno-miR-129
  	hsa-miR-25*	HL
  	hsa-miR-193b	HL
  	hsa-miR-574-5p/mmu-	HL
  	miR-574-5p
  	hsa-miR-589	HL
  	hsa-miR-339-5p/mmu-	HL
  	miR-339-5p/rno-miR-
  	339-5p
  	hsa-miR-34c-5p/mmu-	HL
  	miR-34c/rno-miR-34c
  	hsa-miR-891a	HL
  	hsa-miR-18a/mmu-miR-	HL
  	18a/rno-miR-18a
  	hsa-miR-196a*/mmu-	HL
  	miR-196a*/rno-miR-
  	196a*
  	hsa-miR-17*/rno-miR-	HL
  	17-3p
  	hsa-miR-296-5p/mmu-	HL
  	miR-296-5p/rno-miR-
  	296*
  	hsa-miR-25/mmu-miR-	HL
  	25/rno-miR-25
  	hsa-miR-509-5p	HL
  	hsa-miR-550*	HL
  	hsa-miR-708/mmu-miR-	HL
  	708/rno-miR-708
  	hsa-miR-146b-3p	HL
  	hsa-miR-625*	HL
  	hsa-miR-210/mmu-miR-	HL
  	210/rno-miR-210
  	hsa-miR-93/mmu-miR-	HL
  	93/rno-miR-93
  	hsa-miR-548b-3p	HL
  	hsa-miR-652/mmu-miR-	HL
  	652/rno-miR-652
  	hsa-miR-153/mmu-miR-	HL
  	153/rno-miR-153
  	mghv-miR-M1-3	HL
  	hsa-miR-194*	HL
  	hsa-miR-23a*/rno-miR-	HL
  	23a*
  	hsa-miR-943	HL
  	hsa-let-7d/mmu-let-	HL
  	7d/rno-let-7d
  	hsa-miR-498	HL
  	hsa-miR-381/mmu-miR-	HL
  	381/rno-miR-381
  	hsa-miR-586	HL
  	hsa-miR-137/mmu-miR-	HL
  	137/rno-miR-137
  	hsa-miR-610	HL
  	hsa-miR-920	HL
  	hsa-miR-936	HL
  	hsa-miR-744/mmu-miR-	HL
  	744
  	ebv-miR-BART5	HL
  	hsa-miR-21*	HL
  	hsa-miR-516a-5p	HL
  	hsa-miR-576-5p	HL
  	mghv-miR-M1-6	HL
  	hsa-miR-425/mmu-miR-	HL
  	425/rno-miR-425
  	hsa-miR-220c	HL
  	hsa-miR-10a*/mmu-miR-	HL
  	10a*/rno-miR-10a-3p
  	hsa-miR-452	HL
  	hsa-miR-345	HL
  	hsa-miR-29c*/mmu-miR-	HL
  	29c*/rno-miR-29c*
  	hsa-miR-887	HL
  	hsa-miR-7-2*	HL
  	hsa-miR-363*/rno-miR-	HL
  	363*
  	hsa-miR-22*/mmu-miR-	HL
  	22*/rno-miR-22*
  	hsa-miR-922	HL
  	hsa-miR-92b*	HL
  	hsa-miR-526a/hsa-miR-	HL
  	520c-5p/hsa-miR-518d-
  	5p
  	hsa-miR-574-3p/mmu-	HL
  	miR-574-3p
  	hsa-miR-92a/mmu-miR-	HL
  	92a/rno-miR-92a
  	hsa-miR-423-3p/mmu-	HL
  	miR-423-3p/rno-miR-423
  	hsa-miR-526b	HL
  	hsa-miR-526b*	HL
  	ebv-miR-BART6-3p	HL
  	hsa-miR-92b/mmu-miR-	HL
  	92b/rno-miR-92b
  	hsa-miR-519e*	HL
  	hiv1-miR-H1	HL
  	hsa-miR-623	HL
  	hsa-miR-483-5p	HL
  	mghv-miR-M1-2	HL
  	mghv-miR-M1-7-3p	HL
  	hsa-miR-519e	HL
  	hsa-miR-361-5p/mmu-	HL
  	miR-361/rno-miR-361
  	hsa-miR-650	HL
  	hsa-miR-361-3p	HL
  	hsa-miR-374b*	HL
  	kshv-miR-K12-8	HL
  	hsa-miR-150*/mmu-miR-	HL
  	150*
  	hsa-miR-425*/mmu-miR-	HL
  	425*
  	hsa-miR-135a*/mmu-	HL
  	miR-135a*
  	hsa-miR-612	HL
  	hsa-miR-212/mmu-miR-	HL
  	212/rno-miR-212
  	hsa-miR-125b-2*/rno-	HL
  	miR-125b*
  	hcmv-miR-UL112	HL
  	hsa-miR-500	HL
  	hsa-miR-502-5p	HL
  	ebv-miR-BART18-3p	HL
  	hsa-miR-625	HL
  	hsa-miR-138/mmu-miR-	HL
  	138/rno-miR-138
  	hsa-miR-500*	HL
  	hsa-miR-124*/mmu-miR-	HL
  	124*/rno-miR-124*
  	hsa-miR-516b	HL
  	hsa-miR-30c-1*/mmu-	HL
  	miR-30c-1*/rno-miR-
  	30c-1*
  	hsa-miR-331-5p/mmu-	HL
  	miR-331-5p
  	hsa-miR-510	HL
  	hsa-miR-376a*	HL
  	hsa-miR-640	HL
  	hsa-miR-331-3p/mmu-	HL
  	miR-331-3p/rno-miR-331
  	hsa-miR-602	HL
  	hsa-miR-485-3p/mmu-	HL
  	miR-485*
  	hsa-miR-488	HL
  	hsa-miR-125a-	HL
  	3p/mmu-miR-125a-
  	3p/rno-miR-125a-3p
  	hsa-miR-24-2*/mmu-	HL
  	miR-24-2*/rno-miR-
  	24-2*
  	hsa-miR-484/mmu-	HL
  	miR-484/rno-miR-
  	484
  	hsa-miR-106b*/mmu-	HL
  	miR-106b*/rno-miR-
  	106b*
  	hsa-miR-600	HL
  	hsa-let-7b*/mmu-let-	HL
  	7b*/rno-let-7b*
  	hsa-miR-302c*	HL
  	hsa-miR-20b*	HL
  	hsa-miR-524-5p	HL
  	hsa-miR-505*	HL
  	hsa-miR-542-	HL
  	5p/mmu-miR-542-
  	5p/rno-miR-542-5p
  	hsa-miR-557	HL
  	hsa-miR-183/mmu-	HL
  	miR-183/rno-miR-
  	183
  	hsa-miR-122*	HL
  	hsa-miR-675	HL
  	hsv1-miR-H1	HL
  	hsa-miR-99b/mmu-	HL
  	miR-99b/rno-miR-
  	99b
  	hsa-miR-766	HL
  	hsa-miR-409-	HL
  	5p/mmu-miR-409-
  	5p/rno-miR-409-5p
  	ebv-miR-BART20-3p	HL
  	hsa-miR-129*	HL
  	mghv-miR-M1-7-5p	HL
  	hsa-miR-671-	HL
  	5p/mmu-miR-671-5p
  	hsa-miR-629	HL
  	hsa-miR-553	HL
  	hsa-let-7d*/mmu-let-	HL
  	7d*/rno-let-7d*
  	hsa-miR-601	HL
  	hsa-miR-645	HL
  	hsa-miR-221*	HL
  	hsa-miR-874/mmu-	HL
  	miR-874/rno-miR-
  	874
  	hsa-miR-890	HL
  	hsa-miR-492	HL
  	hsa-miR-629*	HL
  	hsa-miR-635	HL
  	hsa-miR-130b/mmu-	HL
  	miR-130b/rno-miR-
  	130b
  	hsa-miR-197/mmu-	HL
  	miR-197
  	hsa-miR-654-5p	HL
  	hsa-miR-518b	HL
  	hsa-miR-889	HL
  	hsa-miR-584	HL
  	hsa-miR-198	HL
  	hsa-miR-636	HL
  	hsa-miR-630	HL
  	hsa-miR-490-5p	HL
  	hsa-miR-663	HL
  	hcmv-miR-UL148D	HL
  	hsa-miR-337-3p	HL
  	hsa-miR-9*/mmu-	HL
  	miR-9*/rno-miR-9*
  	hsa-miR-200b*/mmu-	HL
  	miR-200b*
  	ebv-miR-BART9*	HL
  	hsa-miR-342-	HL
  	5p/mmu-miR-342-
  	5p/rno-miR-342-5p
  	hsa-miR-206/mmu-	HL
  	miR-206/rno-miR-
  	206
  	hcmv-miR-US25-1*	HL
  	hsa-miR-659	HL
  	hsa-miR-514	HL
  	kshv-miR-K12-6-5p	HL
  	hsa-miR-508-5p	HL
  	hsa-miR-377*	HL
  	ebv-miR-BART16	HL
  	hsa-miR-181b/mmu-	HL
  	miR-181b/rno-miR-
  	181b
  	hsa-miR-622	HL
  	kshv-miR-K12-1	HL
  	hsa-miR-490-	HL
  	3p/mmu-miR-490
  	hsa-miR-125b-	HL
  	1*/mmu-miR-125b-
  	3p/rno-miR-125b-3p
  	hsa-miR-124/mmu-	HL
  	miR-124/rno-miR-
  	124
  	hsa-miR-657	HL
  	ebv-miR-BHRF1-3	HL
  	kshv-miR-K12-5	HL
  	hsa-miR-487b/mmu-	HL
  	miR-487b/rno-miR-
  	487b
  	hsa-miR-183*/mmu-	HL
  	miR-183*
  	hsa-miR-297/mmu-	HL
  	miR-297a
  	hsa-miR-885-5p	HL
  	hsa-miR-296-	HL
  	3p/mmu-miR-296-
  	3p/rno-miR-296
  	ebv-miR-BART19-3p	HL
  	hsa-miR-617	HL
  	hsa-miR-519d	HL
  	hsa-miR-195*	HL
  	hsa-miR-575	HL
  	hsa-miR-208a/mmu-	HL
  	miR-208a/rno-miR-
  	208
  	hsa-miR-647	HL
  	hsa-miR-525-5p	HL
  	ebv-miR-BART8*	HL
  	hsa-miR-340*/mmu-	HL
  	miR-340-3p/rno-miR-
  	340-3p
  	hsa-miR-220b	HL
  	hsa-miR-382/mmu-	HL
  	miR-382/rno-miR-
  	382
  	hsa-miR-585	HL
  	hsa-miR-877/mmu-	HL
  	miR-877/rno-miR-
  	877
  	hsa-miR-99b*/mmu-	HL
  	miR-99b*/rno-miR-
  	99b*
  	ebv-miR-BHRF1-1	HL
  	hsa-miR-326/mmu-	HL
  	miR-326/rno-miR-
  	326
  	ebv-miR-BART7*	HL
  	hsa-miR-615-	HL
  	3p/mmu-miR-615-3p
  	mghv-miR-M1-8	HL
  	hsa-miR-193b*	HL
  	ebv-miR-BART13	HL
  	hsa-miR-433/mmu-	HL
  	miR-433/rno-miR-
  	433
  	hsa-miR-202	HL
  	hsa-miR-551b*	HL
  	hsa-miR-551a	HL
  	hsa-miR-542-	HL
  	3p/mmu-miR-542-
  	3p/rno-miR-542-3p
  	hsa-miR-338-	HL
  	5p/mmu-miR-338-
  	5p/rno-miR-338*
  	hsa-miR-299-3p	HL
  	hsa-miR-518a-3p	HL
  	hsa-miR-181a-2*	HL
  	hsa-miR-938	HL
  	hsa-miR-509-3-5p	HL
  	hsa-miR-552	HL

• TABLE 21
  Predictor microRNAs that distinguish germinal center B-cell like
  (GCB) DLBCL from Burkitt lymphoma

  	GCBvsBL	Higher in
  	hsa-miR-129*	GCB
  	hsa-miR-28-5p/mmu-miR-28/rno-	GCB
  	miR-28
  	hsa-miR-155	GCB
  	hsa-miR-196a*/mmu-miR-196a*/rno-	GCB
  	miR-196a*
  	hsa-miR-146a/mmu-miR-146a/rno-	GCB
  	miR-146a
  	hsa-miR-331-3p/mmu-miR-331-	GCB
  	3p/rno-miR-331
  	hsa-miR-215	GCB
  	hsa-miR-600	GCB
  	mghv-miR-M1-7-3p	GCB
  	hsa-miR-107/mmu-miR-107/rno-	GCB
  	miR-107
  	hsa-miR-886-3p	GCB
  	hsa-miR-140-5p/mmu-miR-140/rno-	GCB
  	miR-140
  	hsa-miR-154/mmu-miR-154/rno-	GCB
  	miR-154
  	hsa-miR-103/mmu-miR-103/rno-	GCB
  	miR-103
  	hsa-let-7g/mmu-let-7g	GCB
  	hsa-miR-222/mmu-miR-222/rno-	GCB
  	miR-222
  	hsa-miR-221/mmu-miR-221/rno-	GCB
  	miR-221
  	hsa-miR-320/mmu-miR-320/rno-	GCB
  	miR-320
  	hsa-miR-140-3p/mmu-miR-140*/rno-	GCB
  	miR-140*
  	hsa-miR-148a/mmu-miR-148a	GCB
  	hsa-miR-365/mmu-miR-365/rno-miR-	GCB
  	365
  	hsa-miR-29c/mmu-miR-29c/rno-miR-	GCB
  	29c
  	hsa-miR-30d/mmu-miR-30d/rno-miR-	GCB
  	30d
  	hsa-miR-214/mmu-miR-214/rno-miR-	GCB
  	214
  	hsa-miR-146b-5p/mmu-miR-146b/rno-	GCB
  	miR-146b
  	hsa-miR-342-3p/mmu-miR-342-3p/rno-	GCB
  	miR-342-3p
  	hsa-miR-374a	GCB
  	hsa-miR-223/mmu-miR-223/rno-miR-	GCB
  	223
  	hsa-miR-29b/mmu-miR-29b/rno-miR-	GCB
  	29b
  	hsa-miR-21/mmu-miR-21/rno-miR-21	GCB
  	hsa-miR-29a/mmu-miR-29a/rno-miR-	GCB
  	29a
  	hsa-miR-23b/mmu-miR-23b/rno-miR-	GCB
  	23b
  	hsa-miR-24/mmu-miR-24/rno-miR-24	GCB
  	hsa-miR-26a/mmu-miR-26a/rno-miR-	GCB
  	26a
  	hsa-miR-26b/mmu-miR-26b/rno-miR-	GCB
  	26b
  	hsa-miR-34b/mmu-miR-34b-3p	GCB
  	hsa-miR-503	BL
  	hsa-miR-30b*	BL

• TABLE 22
  Predictor microRNAs that distinguish germinal center B-cell like
  (GCB) DLBCL from chronic lymphocytic leukemia

  		Higher
  	GCB vs CLL	in
  	hsa-miR-181a/mmu-	GCB
  	miR-181a/rno-miR-
  	181a
  	hsa-miR-886-5p	GCB
  	mghv-miR-M1-7-3p	GCB
  	hsa-miR-934	GCB
  	mghv-miR-M1-3	GCB
  	hsa-miR-485-	GCB
  	3p/mmu-miR-485*
  	hsa-miR-125b/mmu-	GCB
  	miR-125b-5p/rno-
  	miR-125b-5p
  	hsa-miR-637	GCB
  	hsa-miR-365/mmu-	GCB
  	miR-365/rno-miR-
  	365
  	hsa-miR-505*	GCB
  	hsa-miR-199a-	GCB
  	3p/hsa-miR-199b-
  	3p/mmu-miR-199a-
  	3p/mmu-miR-
  	199b/rno-miR-199a-
  	3p
  	hsa-miR-675	GCB
  	hsa-miR-424	GCB
  	ebv-miR-BHRF1-2	GCB
  	hsa-miR-519c-	GCB
  	5p/hsa-miR-519b-
  	5p/hsa-miR-
  	523*/hsa-miR-
  	518e*/hsa-miR-
  	522*/hsa-miR-
  	519a*
  	hsa-miR-130a/mmu-	GCB
  	miR-130a/rno-miR-
  	130a
  	hsa-miR-943	GCB
  	hsa-miR-126/mmu-	GCB
  	miR-126-3p/rno-
  	miR-126
  	hsa-miR-193b	GCB
  	hsa-miR-198	GCB
  	hsa-miR-	GCB
  	200b*/mmu-miR-
  	200b*
  	kshv-miR-K12-6-3p	GCB
  	hsa-miR-220c	GCB
  	hsa-miR-374b*	GCB
  	hsa-miR-518b	GCB
  	hsa-miR-920	GCB
  	hsa-miR-125b-	GCB
  	1*/mmu-miR-125b-
  	3p/rno-miR-125b-3p
  	ebv-miR-BART8*	GCB
  	ebv-miR-BART16	GCB
  	hsa-miR-630	GCB
  	hsa-miR-483-5p	GCB
  	hsa-miR-422a	GCB
  	hsa-miR-526b	GCB
  	hsa-miR-145/mmu-	GCB
  	miR-145/rno-miR-
  	145
  	hsa-miR-126*/mmu-	GCB
  	miR-126-5p/rno-
  	miR-126*
  	hsa-miR-143/mmu-	GCB
  	miR-143/rno-miR-
  	143
  	hsa-miR-100/mmu-	GCB
  	miR-100/rno-miR-100
  	hsa-miR-371-5p	GCB
  	hsa-miR-193a-5p	GCB
  	hsa-miR-628-3p	GCB
  	hsa-miR-185*	GCB
  	hsa-miR-10b/mmu-	GCB
  	miR-10b/rno-miR-10b
  	hsa-miR-665	GCB
  	hsa-miR-503	GCB
  	hsa-miR-642	GCB
  	hsa-miR-658	GCB
  	hsa-miR-21/mmu-	GCB
  	miR-21/rno-miR-21
  	hsa-miR-23a/mmu-	GCB
  	miR-23a/rno-miR-23a
  	hsa-miR-125a-	GCB
  	5p/mmu-miR-125a-
  	5p/rno-miR-125a-5p
  	hsa-miR-24/mmu-	GCB
  	miR-24/rno-miR-24
  	hsa-miR-23b/mmu-	GCB
  	miR-23b/rno-miR-23b
  	hsa-miR-620	GCB
  	hsa-miR-933	GCB
  	hsa-miR-30b*	GCB
  	hsa-miR-22/mmu-	GCB
  	miR-22/rno-miR-22
  	hsa-let-7e/mmu-let-	GCB
  	7e/rno-let-7e
  	mghv-miR-M1-4	GCB
  	hsa-miR-149*	GCB
  	hsa-miR-765	GCB
  	hsa-let-7c/mmu-let-	GCB
  	7c/rno-let-7c
  	hsa-miR-423-	CLL
  	5p/mmu-miR-423-5p
  	hsa-miR-32*	CLL
  	hsa-miR-34b/mmu-	CLL
  	miR-34b-3p
  	hsa-miR-551b/mmu-	CLL
  	miR-551b/rno-miR-
  	551b
  	hsa-let-7i/mmu-let-	CLL
  	7i/rno-let-7i
  	hsa-miR-29a/mmu-	CLL
  	miR-29a/rno-miR-29a
  	hsa-miR-138-	CLL
  	1*/mmu-miR-
  	138*/rno-miR-138*
  	hsa-miR-29b/mmu-	CLL
  	miR-29b/rno-miR-29b
  	hsa-miR-191/mmu-	CLL
  	miR-191/rno-miR-191
  	hsa-miR-26b/mmu-	CLL
  	miR-26b/rno-miR-26b
  	hsa-miR-26a/mmu-	CLL
  	miR-26a/rno-miR-26a
  	hsa-miR-550	CLL
  	hsa-miR-374a	CLL
  	hsa-miR-142-5p/mmu-	CLL
  	miR-142-5p/rno-miR-
  	142-5p
  	hsa-miR-29c/mmu-	CLL
  	miR-29c/rno-miR-29c
  	hsa-miR-140-3p/mmu-	CLL
  	miR-140*/rno-miR-
  	140*
  	hsa-miR-30e/mmu-	CLL
  	miR-30e/rno-miR-30e
  	hsa-miR-801/mmu-	CLL
  	miR-801
  	hsa-miR-768-3p	CLL
  	hsa-miR-549	CLL
  	hsa-miR-199a-5p/mmu-	CLL
  	miR-199a-5p/rno-miR-
  	199a-5p
  	hsa-miR-223/mmu-	CLL
  	miR-223/rno-miR-223
  	hsa-miR-101/mmu-	CLL
  	miR-101a/rno-miR-
  	101a
  	hsa-miR-888*	CLL
  	hsa-miR-24-1*/mmu-	CLL
  	miR-24-1*/rno-miR-24-
  	1*
  	hsa-miR-519d	CLL
  	hsa-miR-154/mmu-	CLL
  	miR-154/rno-miR-154
  	hsa-miR-638	CLL
  	hsa-miR-668/mmu-	CLL
  	miR-668
  	hsa-miR-891a	CLL
  	hsa-miR-768-5p	CLL
  	hsa-miR-140-5p/mmu-	CLL
  	miR-140/rno-miR-140
  	hsa-miR-196a*/mmu-	CLL
  	miR-196a*/rno-miR-
  	196a*
  	hsa-miR-150/mmu-	CLL
  	miR-150/rno-miR-150
  	hsa-let-7g/mmu-let-7g	CLL
  	hsa-miR-363/mmu-	CLL
  	miR-363/rno-miR-363
  	hsa-miR-486-5p/mmu-	CLL
  	miR-486
  	hsa-miR-32/mmu-miR-	CLL
  	32/rno-miR-32
  	hsa-miR-147	CLL
  	hsa-miR-20b*	CLL
  	hsa-miR-487b/mmu-	CLL
  	miR-487b/rno-miR-
  	487b
  	hsa-miR-636	CLL
  	hsa-miR-144*	CLL
  	hsa-miR-186/mmu-	CLL
  	miR-186/rno-miR-186
  	hsa-miR-30e*/mmu-	CLL
  	miR-30e*/rno-miR-
  	30e*
  	hsa-miR-331-5p/mmu-	CLL
  	miR-331-5p

• TABLE 23
  Predictor microRNAs that distinguish germinal center B-cell like
  (GCB) DLBCL from follicular lymphoma

  		Higher
  	GCB vs FL	in
  	hsa-miR-378/mmu-	GCB
  	miR-378/rno-miR-378
  	hsa-miR-20b/mmu-	GCB
  	miR-20b/rno-miR-20b-
  	5p
  	hsa-miR-19b/mmu-	GCB
  	miR-19b/rno-miR-19b
  	hsa-miR-106a	GCB
  	hsa-miR-17/mmu-miR-	GCB
  	17/rno-miR-17-5p/rno-
  	miR-17
  	hsa-miR-93/mmu-miR-	GCB
  	93/rno-miR-93
  	hsa-miR-20a/mmu-	GCB
  	miR-20a/rno-miR-20a
  	hsa-miR-23b/mmu-	GCB
  	miR-23b/rno-miR-23b
  	hsa-miR-23a/mmu-	GCB
  	miR-23a/rno-miR-23a
  	hsa-miR-22/mmu-miR-	GCB
  	22/rno-miR-22
  	hsa-miR-19a/mmu-	GCB
  	miR-19a/rno-miR-19a
  	hsa-miR-320/mmu-	GCB
  	miR-320/rno-miR-320
  	hsa-miR-106b/mmu-	GCB
  	miR-106b/rno-miR-
  	106b
  	hsa-miR-103/mmu-	GCB
  	miR-103/rno-miR-103
  	hsa-miR-30c/mmu-	GCB
  	miR-30c/rno-miR-30c
  	ebv-miR-BHRF1-2	GCB
  	hsa-miR-125a-	GCB
  	5p/mmu-miR-125a-
  	5p/rno-miR-125a-5p
  	hsa-let-7a/mmu-let-	GCB
  	7a/rno-let-7a
  	hsa-miR-628-3p	GCB
  	hsa-let-7c/mmu-let-	GCB
  	7c/rno-let-7c
  	hsa-miR-423-5p/mmu-	FL
  	miR-423-5p
  	hsa-miR-24-1*/mmu-	FL
  	miR-24-1*/rno-miR-
  	24-1*
  	ebv-miR-BART2-3p	FL
  	hsa-miR-138-1*/mmu-	FL
  	miR-138*/rno-miR-
  	138*
  	hsa-miR-768-5p	FL
  	hsa-miR-30b*	FL
  	hsa-miR-494/mmu-	FL
  	miR-494/rno-miR-494
  	hsa-miR-583	FL
  	hsa-miR-185/mmu-	FL
  	miR-185/rno-miR-185
  	hsa-miR-765	FL
  	hsa-miR-34b/mmu-	FL
  	miR-34b-3p
  	hsa-miR-921	FL
  	hsa-miR-551b/mmu-	FL
  	miR-551b/rno-miR-
  	551b
  	hsa-miR-549	FL
  	hsa-miR-939	FL
  	hsa-miR-302d*	FL
  	ebv-miR-BART17-5p	FL
  	hsa-miR-801/mmu-	FL
  	miR-801
  	hsa-miR-888*	FL
  	hsa-miR-620	FL
  	hsa-miR-576-3p	FL
  	hsa-miR-32*	FL
  	hsa-miR-574-5p/mmu-	FL
  	miR-574-5p
  	hsa-miR-505/rno-miR-	FL
  	505
  	hsa-miR-885-5p	FL
  	hsa-miR-455-3p	FL
  	hsa-miR-152/mmu-	FL
  	miR-152/rno-miR-152
  	hsa-miR-200b*/mmu-	FL
  	miR-200b*
  	mghv-miR-M1-2	FL
  	hcmv-miR-UL148D	FL
  	hsa-miR-497/mmu-miR-	FL
  	497/rno-miR-497
  	ebv-miR-BART13	FL
  	ebv-miR-BART16	FL
  	hsa-miR-125b-2*/rno-	FL
  	miR-125b*
  	hsa-miR-490-3p/mmu-	FL
  	miR-490
  	hsa-miR-99b/mmu-miR-	FL
  	99b/rno-miR-99b
  	hsa-miR-339-5p/mmu-	FL
  	miR-339-5p/rno-miR-
  	339-5p
  	hsa-miR-574-3p/mmu-	FL
  	miR-574-3p
  	hsa-miR-515-5p	FL
  	hsa-miR-877/mmu-miR-	FL
  	877/rno-miR-877
  	hsa-miR-208a/mmu-miR-	FL
  	208a/rno-miR-208
  	hcmv-miR-US25-1*	FL
  	hsa-miR-326/mmu-miR-	FL
  	326/rno-miR-326
  	hsa-miR-488	FL
  	hsa-miR-629	FL
  	hsa-miR-24-2*/mmu-	FL
  	miR-24-2*/rno-miR-24-
  	2*
  	hsa-miR-124/mmu-miR-	FL
  	124/rno-miR-124
  	hsa-miR-493	FL
  	hsv1-miR-H1	FL
  	hsa-miR-484/mmu-miR-	FL
  	484/rno-miR-484
  	hsa-miR-483-3p	FL
  	hsa-miR-27a*/mmu-miR-	FL
  	27a*/rno-miR-27a*
  	hsa-miR-144*	FL
  	hsa-miR-617	FL
  	hsa-miR-377*	FL
  	hsa-miR-363*/rno-miR-	FL
  	363*
  	hsa-miR-148b/mmu-miR-	FL
  	148b/rno-miR-148b-3p
  	kshv-miR-K12-1	FL
  	hsa-miR-7/mmu-miR-	FL
  	7a/rno-miR-7a
  	hsa-miR-193b*	FL
  	hsa-miR-542-3p/mmu-	FL
  	miR-542-3p/rno-miR-
  	542-3p
  	hsa-miR-601	FL
  	hsa-miR-106b*/mmu-	FL
  	miR-106b*/rno-miR-	FL
  	106b*
  	hsa-miR-7-2*	FL
  	kshv-miR-K12-6-5p	FL
  	hsa-miR-645	FL
  	hsa-miR-524-5p	FL
  	hsa-miR-548d-5p	FL
  	hsa-miR-9*/mmu-miR-	FL
  	9*/rno-miR-9*
  	hsa-miR-92a/mmu-miR-	FL
  	92a/rno-miR-92a
  	hsa-miR-22*/mmu-miR-	FL
  	22*/rno-miR-22*
  	hsa-miR-500*	FL
  	hsa-miR-890	FL
  	hsa-miR-297/mmu-miR-	FL
  	297a
  	hsa-miR-197/mmu-miR-	FL
  	197
  	hsa-miR-20b*	FL
  	hsa-miR-629*	FL
  	hsa-miR-887	FL
  	hsa-miR-425/mmu-	FL
  	miR-425/rno-miR-425
  	hsa-miR-99b*/mmu-	FL
  	miR-99b*/rno-miR-
  	99b*
  	hsa-miR-513a-3p	FL
  	hsa-miR-206/mmu-	FL
  	miR-206/rno-miR-206
  	hsa-miR-155*	FL
  	hsa-miR-181b/mmu-	FL
  	miR-181b/rno-miR-
  	181b
  	hsa-miR-299-3p	FL
  	hsa-miR-218-2*/mmu-	FL
  	miR-218-2*/rno-miR-
  	218*
  	mghv-miR-M1-8	FL
  	ebv-miR-BART18-3p	FL
  	hsa-miR-708/mmu-	FL
  	miR-708/rno-miR-708
  	hsa-miR-409-5p/mmu-	FL
  	miR-409-5p/rno-miR-
  	409-5p
  	hsa-miR-553	FL
  	hsa-miR-361-3p	FL
  	hsa-miR-296-3p/mmu-	FL
  	miR-296-3p/rno-miR-
  	296
  	ebv-miR-BHRF1-3	FL
  	hsa-miR-34c-5p/mmu-	FL
  	miR-34c/rno-miR-34c
  	hsa-let-7b*/mmu-let-	FL
  	7b*/rno-let-7b*
  	hsa-miR-301a/mmu-	FL
  	miR-301a/rno-miR-
  	301a
  	hsa-miR-122*	FL
  	hsa-miR-183/mmu-	FL
  	miR-183/rno-miR-183
  	kshv-miR-K12-5	FL
  	kshv-miR-K12-7	FL
  	hsa-miR-552	FL
  	hsa-miR-151-3p	FL
  	hsa-miR-194/mmu-	FL
  	miR-194/rno-miR-194
  	hsa-miR-585	FL
  	hsa-miR-340*/mmu-	FL
  	miR-340-3p/rno-miR-
  	340-3p
  	hsa-let-7d*/mmu-let-	FL
  	7d*/rno-let-7d*
  	hsa-miR-622	FL
  	hsa-miR-575	FL
  	hsa-miR-514	FL
  	hsa-miR-92b/mmu-	FL
  	miR-92b/rno-miR-92b
  	hsa-miR-551a	FL
  	hsa-miR-221*	FL
  	hsa-miR-922	FL
  	hsa-miR-938	FL
  	hsa-miR-615-3p/mmu-	FL
  	miR-615-3p
  	hsa-miR-220b	FL
  	hsa-miR-744/mmu-	FL
  	miR-744
  	hsa-miR-657	FL
  	hsa-miR-382/mmu-	FL
  	miR-382/rno-miR-382
  	hsa-miR-518a-3p	FL
  	hsa-miR-138/mmu-	FL
  	miR-138/rno-miR-138
  	hsa-miR-636	FL
  	hsa-miR-96/mmu-	FL
  	miR-96/rno-miR-96
  	hsa-miR-509-3-5p	FL
  	hsa-miR-337-3p	FL
  	hsa-miR-342-5p/mmu-	FL
  	miR-342-5p/rno-miR-
  	342-5p

• TABLE 24
  Predictor microRNAs that distinguish germinal center B-cell like
  (GCB) DLBCL from Hodgkin's lymphoma

  		Higher
  	GCB vs HL	in
  	hsa-miR-19b/mmu-miR-	GCB
  	19b/rno-miR-19b
  	hsa-miR-19a/mmu-miR-	GCB
  	19a/rno-miR-19a
  	hsa-miR-106a	GCB
  	hsa-miR-20b/mmu-miR-	GCB
  	20b/rno-miR-20b-5p
  	hsa-miR-17/mmu-miR-	GCB
  	17/rno-miR-17-5p/rno-
  	miR-17
  	hsa-miR-15b/mmu-miR-	GCB
  	15b/rno-miR-15b
  	hsa-miR-20a/mmu-miR-	GCB
  	20a/rno-miR-20a
  	hsa-miR-30b/mmu-miR-	GCB
  	30b/rno-miR-30b-5p
  	hsa-miR-142-3p/mmu-miR-	GCB
  	142-3p/rno-miR-142-3p
  	hsa-miR-30c/mmu-miR-	GCB
  	30c/rno-miR-30c
  	hsa-miR-378/mmu-miR-	GCB
  	378/rno-miR-378
  	hsa-miR-93/mmu-miR-	GCB
  	93/rno-miR-93
  	hsa-miR-106b/mmu-miR-	GCB
  	106b/rno-miR-106b
  	hsa-miR-374a	GCB
  	hsa-miR-24/mmu-miR-	GCB
  	24/rno-miR-24
  	hsa-miR-29b/mmu-miR-	GCB
  	29b/rno-miR-29b
  	hsa-miR-22/mmu-miR-	GCB
  	22/rno-miR-22
  	hsa-miR-142-5p/mmu-miR-	GCB
  	142-5p/rno-miR-142-5p
  	hsa-miR-30e/mmu-miR-	GCB
  	30e/rno-miR-30e
  	hsa-miR-30a/mmu-miR-	GCB
  	30a/rno-miR-30a
  	hsa-miR-30d/mmu-miR-	GCB
  	30d/rno-miR-30d
  	hsa-miR-23b/mmu-miR-	GCB
  	23b/rno-miR-23b
  	hsa-miR-16/mmu-miR-	GCB
  	16/rno-miR-16
  	hsa-miR-191/mmu-miR-	GCB
  	191/rno-miR-191
  	hsa-miR-15a/mmu-miR-	GCB
  	15a
  	hsa-miR-26b/mmu-miR-	GCB
  	26b/rno-miR-26b
  	hsa-miR-23a/mmu-miR-	GCB
  	23a/rno-miR-23a
  	hsa-let-7a/mmu-let-7a/rno-	GCB
  	let-7a
  	hsa-miR-103/mmu-miR-	GCB
  	103/rno-miR-103
  	hsa-miR-140-3p/mmu-miR-	GCB
  	140*/rno-miR-140*
  	hsa-miR-154/mmu-miR-	GCB
  	154/rno-miR-154
  	hsa-miR-320/mmu-miR-	GCB
  	320/rno-miR-320
  	hsa-miR-550	GCB
  	hsa-miR-125a-5p/mmu-	GCB
  	miR-125a-5p/rno-miR-
  	125a-5p
  	hsa-let-7c/mmu-let-7c/rno-	GCB
  	let-7c
  	hsa-miR-185/mmu-miR-	HL
  	185/rno-miR-185
  	hsa-miR-658	HL
  	hsa-miR-549	HL
  	hsa-miR-634	HL
  	hsa-miR-551b/mmu-miR-	HL
  	551b/rno-miR-551b
  	hsa-miR-518c*	HL
  	hsa-miR-888*	HL
  	hsa-miR-765	HL
  	hsa-miR-423-5p/mmu-miR-	HL
  	423-5p
  	hsa-miR-30c-2*/mmu-miR-	HL
  	30c-2*/rno-miR-30c-2*
  	hsa-miR-503	HL
  	hsa-miR-921	HL
  	hsa-miR-520d-5p	HL
  	hsa-miR-574-5p/mmu-miR-	HL
  	574-5p
  	hsa-miR-32*	HL
  	hsa-miR-939	HL
  	ebv-miR-BART2-3p	HL
  	ebv-miR-BHRF1-2	HL
  	hsa-miR-583	HL
  	hsa-miR-30b*	HL
  	hsa-miR-149*	HL
  	mghv-miR-M1-4	HL
  	hsa-miR-513a-5p	HL
  	hsa-miR-494/mmu-miR-	HL
  	494/rno-miR-494
  	hsa-miR-498	HL
  	hsa-miR-485-3p/mmu-miR-	HL
  	485*
  	hsa-miR-129-5p/mmu-miR-	HL
  	129-5p/rno-miR-129
  	hsa-miR-25*	HL
  	hsa-miR-923	HL
  	hsa-miR-519d	HL
  	hsa-miR-516a-5p	HL
  	hsa-miR-99a/mmu-miR-	HL
  	99a/rno-miR-99a
  	hsa-miR-943	HL
  	hsa-miR-885-5p	HL
  	ebv-miR-BHRF1-1	HL
  	hsa-miR-152/mmu-miR-	HL
  	152/rno-miR-152
  	ebv-miR-BART8*	HL
  	hsa-miR-200b*/mmu-miR-	HL
  	200b*
  	hsa-miR-125b-1*/mmu-	HL
  	miR-125b-3p/rno-miR-
  	125b-3p
  	hsa-miR-526b	HL
  	hsa-miR-29a*/mmu-miR-	HL
  	29a*/rno-miR-29a*
  	hsa-miR-532-5p/mmu-miR-	HL
  	532-5p/rno-miR-532-5p
  	hsa-miR-183*/mmu-miR-	HL
  	183*
  	hsa-miR-659	HL
  	hsa-miR-145*/mmu-miR-	HL
  	145*
  	mghv-miR-M1-7-5p	HL
  	hsa-miR-143*	HL
  	hsa-miR-660	HL
  	hsa-miR-130b/mmu-	HL
  	miR-130b/rno-miR-130b
  	hsa-miR-671-5p/mmu-	HL
  	miR-671-5p
  	hsa-miR-525-5p	HL
  	hsa-miR-505/rno-miR-	HL
  	505
  	hsa-miR-488	HL
  	hsa-miR-766	HL
  	hsa-miR-20b*	HL
  	hsa-miR-339-5p/mmu-	HL
  	miR-339-5p/rno-miR-
  	339-5p
  	hsa-miR-524-5p	HL
  	hsa-miR-455-3p	HL
  	hsa-miR-92a/mmu-miR-	HL
  	92a/rno-miR-92a
  	hsa-miR-502-3p	HL
  	hsa-miR-210/mmu-miR-	HL
  	210/rno-miR-210
  	hsv1-miR-H1	HL
  	ebv-miR-BART6-3p	HL
  	hsa-miR-490-3p/mmu-	HL
  	miR-490
  	hsa-miR-149/mmu-miR-	HL
  	149
  	hsa-miR-128/mmu-miR-	HL
  	128/rno-miR-128
  	hsa-miR-635	HL
  	hcmv-miR-UL148D	HL
  	hsa-miR-373*	HL
  	hsa-miR-647	HL
  	hsa-miR-197/mmu-miR-	HL
  	197
  	hsa-miR-602	HL
  	hsa-miR-656	HL
  	hsa-miR-874/mmu-miR-	HL
  	874/rno-miR-874
  	ebv-miR-BART19-3p	HL
  	hsa-miR-551b*	HL
  	hsa-miR-96/mmu-miR-	HL
  	96/rno-miR-96
  	hsa-miR-889	HL
  	hsa-miR-425/mmu-miR-	HL
  	425/rno-miR-425
  	hsa-miR-34c-5p/mmu-	HL
  	miR-34c/rno-miR-34c
  	hcmv-miR-UL70-3p	HL
  	hsa-miR-27a*/mmu-	HL
  	miR-27a*/rno-miR-27a*
  	hsa-miR-194/mmu-miR-	HL
  	194/rno-miR-194
  	hsa-miR-17*/rno-miR-	HL
  	17-3p
  	hsa-miR-548d-5p	HL
  	hsa-miR-7/mmu-miR-	HL
  	7a/rno-miR-7a
  	hsa-miR-877/mmu-miR-	HL
  	877/rno-miR-877
  	hsa-miR-22*/mmu-miR-	HL
  	22*/rno-miR-22*
  	hsa-miR-323-3p/mmu-	HL
  	miR-323-3p/rno-miR-
  	323
  	hsa-miR-708/mmu-miR-	HL
  	708/rno-miR-708
  	hsa-miR-513a-3p	HL
  	hsa-miR-595	HL
  	hsa-miR-922	HL
  	hsa-miR-515-5p	HL
  	hsa-miR-99b/mmu-miR-	HL
  	99b/rno-miR-99b
  	hsa-miR-483-3p	HL
  	hsa-miR-330-5p/mmu-	HL
  	miR-330/rno-miR-330
  	hsa-miR-509-3p	HL
  	hsa-miR-151-3p	HL
  	ebv-miR-BART13	HL
  	hsa-miR-617	HL
  	hsa-miR-328/mmu-miR-	HL
  	328/rno-miR-328
  	hsa-miR-361-3p	HL
  	hsa-miR-138/mmu-miR-	HL
  	138/rno-miR-138
  	ebv-miR-BART7*	HL
  	hsa-miR-589	HL
  	hsa-miR-576-5p	HL
  	hsa-miR-452	HL
  	hsa-miR-7-2*	HL
  	hsa-miR-296-5p/mmu-	HL
  	miR-296-5p/rno-miR-
  	296*
  	hsa-miR-550*	HL
  	hsa-miR-92b/mmu-miR-	HL
  	92b/rno-miR-92b
  	kshv-miR-K12-1	HL
  	hsa-miR-526a/hsa-miR-	HL
  	520c-5p/hsa-miR-518d-
  	5p
  	mghv-miR-M1-2	HL
  	hsa-miR-548b-3p	HL
  	hsa-miR-297/mmu-miR-	HL
  	297a
  	hsa-miR-195*	HL
  	hsa-miR-652/mmu-miR-	HL
  	652/rno-miR-652
  	hsa-miR-221*	HL
  	hsa-miR-194*	HL
  	hsa-miR-23a*/rno-miR-	HL
  	23a*
  	hsa-miR-125b-2*/rno-	HL
  	miR-125b*
  	hsa-miR-212/mmu-miR-	HL
  	212/rno-miR-212
  	ebv-miR-BART18-3p	HL
  	hsa-miR-586	HL
  	hsa-miR-137/mmu-miR-	HL
  	137/rno-miR-137
  	hsa-miR-610	HL
  	hcmv-miR-UL112	HL
  	hsa-miR-181b/mmu-	HL
  	miR-181b/rno-miR-181b
  	hsa-miR-936	HL
  	hsa-miR-744/mmu-	HL
  	miR-744
  	kshv-miR-K12-6-5p	HL
  	hcmv-miR-US25-1*	HL
  	hsa-miR-21*	HL
  	mghv-miR-M1-6	HL
  	mghv-miR-M1-8	HL
  	hsa-miR-10a*/mmu-	HL
  	miR-10a*/rno-miR-
  	10a-3p
  	hsa-miR-345	HL
  	hsa-miR-887	HL
  	hsa-miR-193b*	HL
  	hsa-miR-122*	HL
  	kshv-miR-K12-5	HL
  	hsa-miR-92b*	HL
  	hsa-miR-526b*	HL
  	hsa-miR-553	HL
  	hsa-miR-601	HL
  	hiv1-miR-H1	HL
  	hsa-miR-623	HL
  	hsa-miR-519e	HL
  	hsa-miR-650	HL
  	hsa-miR-575	HL
  	hsa-miR-629*	HL
  	hsa-miR-890	HL
  	hsa-miR-	HL
  	150*/mmu-miR-
  	150*
  	hsa-miR-	HL
  	425*/mmu-miR-
  	425*
  	hsa-miR-	HL
  	135a*/mmu-miR-
  	135a*
  	hsa-miR-612	HL
  	hsa-miR-636	HL
  	hsa-miR-500	HL
  	hsa-miR-502-5p	HL
  	hsa-miR-9*/mmu-	HL
  	miR-9*/rno-miR-9*
  	hsa-miR-500*	HL
  	hsa-miR-	HL
  	124*/mmu-miR-
  	124*/rno-miR-124*
  	hsa-miR-30c-	HL
  	1*/mmu-miR-30c-
  	1*/rno-miR-30c-1*
  	hsa-miR-	HL
  	99b*/mmu-miR-
  	99b*/rno-miR-99b*
  	hsa-miR-331-	HL
  	5p/mmu-miR-331-
  	5p
  	hsa-miR-206/mmu-	HL
  	miR-206/rno-miR-
  	206
  	hsa-miR-376a*	HL
  	hsa-miR-585	HL
  	hsa-miR-640	HL
  	hsa-miR-377*	HL
  	hsa-miR-125a-	HL
  	3p/mmu-miR-125a-
  	3p/rno-miR-125a-3p
  	hsa-miR-24-	HL
  	2*/mmu-miR-24-
  	2*/rno-miR-24-2*
  	hsa-miR-484/mmu-	HL
  	miR-484/rno-miR-
  	484
  	hsa-miR-	HL
  	106b*/mmu-miR-
  	106b*/rno-miR-
  	106b*
  	hsa-let-7b*/mmu-	HL
  	let-7b*/rno-let-7b*
  	hsa-miR-302c*	HL
  	hsa-miR-542-	HL
  	5p/mmu-miR-542-
  	5p/rno-miR-542-5p
  	hsa-miR-622	HL
  	ebv-miR-BHRF1-3	HL
  	hsa-miR-181a-2*	HL
  	hsa-miR-183/mmu-	HL
  	miR-183/rno-miR-
  	183
  	hsa-miR-409-	HL
  	5p/mmu-miR-409-
  	5p/rno-miR-409-5p
  	ebv-miR-BART20-	HL
  	3p
  	hsa-miR-629	HL
  	hsa-let-7d*/mmu-	HL
  	let-7d*/rno-let-7d*
  	hsa-miR-645	HL
  	hsa-miR-492	HL
  	hsa-miR-654-5p	HL
  	hsa-miR-208a/mmu-	HL
  	miR-208a/rno-miR-
  	208
  	hsa-miR-584	HL
  	hsa-miR-382/mmu-	HL
  	miR-382/rno-miR-
  	382
  	hsa-miR-	HL
  	340*/mmu-miR-
  	340-3p/rno-miR-
  	340-3p
  	hsa-miR-490-5p	HL
  	hsa-miR-663	HL
  	hsa-miR-337-3p	HL
  	hsa-miR-518a-3p	HL
  	ebv-miR-BART9*	HL
  	hsa-miR-342-	HL
  	5p/mmu-miR-342-
  	5p/rno-miR-342-5p
  	hsa-miR-514	HL
  	hsa-miR-508-5p	HL
  	hsa-miR-124/mmu-	HL
  	miR-124/rno-miR-
  	124
  	hsa-miR-657	HL
  	hsa-miR-938	HL
  	hsa-miR-296-	HL
  	3p/mmu-miR-296-
  	3p/rno-miR-296
  	hsa-miR-551a	HL
  	hsa-miR-542-	HL
  	3p/mmu-miR-542-
  	3p/rno-miR-542-3p
  	hsa-miR-220b	HL
  	hsa-miR-326/mmu-	HL
  	miR-326/rno-miR-
  	326
  	hsa-miR-615-	HL
  	3p/mmu-miR-615-
  	3p
  	hsa-miR-433/mmu-	HL
  	miR-433/rno-miR-
  	433
  	hsa-miR-202	HL
  	hsa-miR-338-	HL
  	5p/mmu-miR-338-
  	5p/rno-miR-338*
  	hsa-miR-552	HL
  	hsa-miR-299-3p	HL
  	hsa-miR-509-3-5p	HL

• TABLE 25
  Predictor microRNAs that distinguish Burkitt lymphoma
  from chromic lymphocytic leukemia

  		Higher
  	BL vs CLL	in
  	hsa-miR-874/mmu-	BL
  	miR-874/rno-miR-874
  	hsa-miR-125b/mmu-	BL
  	miR-125b-5p/rno-miR-
  	125b-5p
  	hsa-miR-126/mmu-	BL
  	miR-126-3p/rno-miR-
  	126
  	ebv-miR-BHRF1-2	BL
  	hsa-miR-193b	BL
  	hsa-miR-371-5p	BL
  	hsa-miR-193a-5p	BL
  	hsa-miR-628-3p	BL
  	hsa-miR-185*	BL
  	hsa-miR-503	BL
  	hsa-miR-199a-3p/hsa-	BL
  	miR-199b-3p/mmu-
  	miR-199a-3p/mmu-
  	miR-199b/rno-miR-
  	199a-3p
  	hsa-miR-143/mmu-	BL
  	miR-143/rno-miR-143
  	hsa-miR-130a/mmu-	BL
  	miR-130a/rno-miR-
  	130a
  	hsa-miR-145/mmu-	BL
  	miR-145/rno-miR-145
  	hsa-miR-30b*	BL
  	hsa-miR-665	BL
  	hsa-miR-658	BL
  	hsa-miR-933	BL
  	hsa-miR-30c-2*/mmu-	BL
  	miR-30c-2*/rno-miR-
  	30c-2*
  	hsa-miR-765	BL
  	hsa-miR-620	BL
  	hsa-miR-520d-5p	BL
  	hsa-miR-494/mmu-	BL
  	miR-494/rno-miR-494
  	hsa-miR-551b/mmu-	CLL
  	miR-551b/rno-miR-
  	551b
  	hsa-miR-106b/mmu-	CLL
  	miR-106b/rno-miR-
  	106b
  	hsa-miR-30c/mmu-	CLL
  	miR-30c/rno-miR-30c
  	hsa-miR-16/mmu-miR-	CLL
  	16/rno-miR-16
  	hsa-miR-27a/mmu-	CLL
  	miR-27a/rno-miR-27a
  	hsa-miR-27b/mmu-	CLL
  	miR-27b/rno-miR-27b
  	hsa-miR-550	CLL
  	hsa-miR-30a/mmu-	CLL
  	miR-30a/rno-miR-30a
  	hsa-miR-30b/mmu-miR-	CLL
  	30b/rno-miR-30b-5p
  	hsa-miR-34b/mmu-miR-	CLL
  	34b-3p
  	hsa-miR-801/mmu-miR-	CLL
  	801
  	hsa-miR-26b/mmu-miR-	CLL
  	26b/rno-miR-26b
  	hsa-let-7b/mmu-let-	CLL
  	7b/rno-let-7b
  	hsa-miR-142-5p/mmu-	CLL
  	miR-142-5p/rno-miR-
  	142-5p
  	hsa-miR-26a/mmu-miR-	CLL
  	26a/rno-miR-26a
  	hsa-miR-29a/mmu-miR-	CLL
  	29a/rno-miR-29a
  	hsa-miR-768-3p	CLL
  	hsa-miR-199a-5p/mmu-	CLL
  	miR-199a-5p/rno-miR-
  	199a-5p
  	hsa-miR-30e/mmu-miR-	CLL
  	30e/rno-miR-30e
  	hsa-miR-29b/mmu-miR-	CLL
  	29b/rno-miR-29b
  	hsa-miR-101/mmu-miR-	CLL
  	101a/rno-miR-101a
  	hsa-miR-138-1*/mmu-	CLL
  	miR-138*/rno-miR-138*
  	hsa-miR-195/mmu-miR-	CLL
  	195/rno-miR-195
  	hsa-miR-549	CLL
  	hsa-miR-103/mmu-miR-	CLL
  	103/rno-miR-103
  	hsa-miR-649	CLL
  	hsa-miR-335/mmu-miR-	CLL
  	335-5p/rno-miR-335
  	hsa-miR-342-3p/mmu-	CLL
  	miR-342-3p/rno-miR-
  	342-3p
  	hsa-miR-423-3p/mmu-	CLL
  	miR-423-3p/rno-miR-
  	423
  	hsa-miR-222/mmu-miR-	CLL
  	222/rno-miR-222
  	hsa-miR-374a	CLL
  	hsa-miR-888*	CLL
  	hsa-miR-30d/mmu-miR-	CLL
  	30d/rno-miR-30d
  	hsa-miR-299-5p/mmu-	CLL
  	miR-299*/rno-miR-299
  	hsa-miR-107/mmu-miR-	CLL
  	107/rno-miR-107
  	hsa-miR-105	CLL
  	hsa-let-7f/mmu-let-	CLL
  	7f/rno-let-7f
  	hsa-miR-191/mmu-miR-	CLL
  	191/rno-miR-191
  	hsa-miR-223/mmu-miR-	CLL
  	223/rno-miR-223
  	hsa-miR-361-5p/mmu-	CLL
  	miR-361/rno-miR-361
  	hsa-miR-29c/mmu-	CLL
  	miR-29c/rno-miR-29c
  	hsa-miR-147	CLL
  	hsa-miR-361-3p	CLL
  	hsa-miR-140-3p/mmu-	CLL
  	miR-140*/rno-miR-
  	140*
  	hsa-miR-486-5p/mmu-	CLL
  	miR-486
  	hsa-miR-33a/mmu-	CLL
  	miR-33/rno-miR-33
  	hsa-miR-636	CLL
  	hsa-miR-24-1*/mmu-	CLL
  	miR-24-1*/rno-miR-24-
  	1*
  	hsa-miR-144*	CLL
  	hsa-miR-668/mmu-	CLL
  	miR-668
  	hsa-miR-768-5p	CLL
  	hsa-miR-363/mmu-	CLL
  	miR-363/rno-miR-363
  	hsa-miR-150/mmu-	CLL
  	miR-150/rno-miR-150
  	hsa-miR-519d	CLL
  	hsa-miR-891a	CLL
  	hsa-miR-186/mmu-	CLL
  	miR-186/rno-miR-186
  	hsa-miR-331-5p/mmu-	CLL
  	miR-331-5p
  	hsa-miR-28-5p/mmu-	CLL
  	miR-28/rno-miR-28
  	hsa-miR-154/mmu-	CLL
  	miR-154/rno-miR-154
  	hsa-miR-155	CLL
  	hsa-miR-363*/rno-miR-	CLL
  	363*
  	hsa-miR-32/mmu-miR-	CLL
  	32/rno-miR-32
  	hsa-miR-30e*/mmu-	CLL
  	miR-30e*/rno-miR-
  	30e*
  	hsa-miR-140-5p/mmu-	CLL
  	miR-140/rno-miR-140
  	hsa-let-7g/mmu-let-7g	CLL
  	hsa-miR-20b*	CLL
  	hsa-miR-129*	CLL
  	hsa-miR-196a*/mmu-	CLL
  	miR-196a*/rno-miR-
  	196a*
  	hsa-miR-487b/mmu-	CLL
  	miR-487b/rno-miR-
  	487b

• TABLE 26
  Predictor microRNAs that distinguish Burkitt lymphoma
  from follicular lymphoma

  		Higher
  	BL vs FL	in
  	hsa-miR-17/mmu-miR-	BL
  	17/rno-miR-17-5p/rno-
  	miR-17
  	hsa-miR-106a	BL
  	hsa-miR-19b/mmu-miR-	BL
  	19b/rno-miR-19b
  	hsa-miR-20a/mmu-miR-	BL
  	20a/rno-miR-20a
  	hsa-miR-19a/mmu-miR-	BL
  	19a/rno-miR-19a
  	hsa-miR-628-3p	BL
  	hsa-miR-503	BL
  	hsa-miR-371-5p	BL
  	hsa-miR-106b/mmu-	BL
  	miR-106b/rno-miR-106b
  	hsa-miR-30c-2*/mmu-	BL
  	miR-30c-2*/rno-miR-
  	30c-2*
  	ebv-miR-BART2-3p	FL
  	hsa-let-7e/mmu-let-	FL
  	7e/rno-let-7e
  	hsa-miR-551b/mmu-	FL
  	miR-551b/rno-miR-551b
  	hsa-miR-26b/mmu-miR-	FL
  	26b/rno-miR-26b
  	hsa-miR-26a/mmu-miR-	FL
  	26a/rno-miR-26a
  	hsa-miR-620	FL
  	hsa-miR-801/mmu-miR-	FL
  	801
  	ebv-miR-BART17-5p	FL
  	hsa-miR-29a/mmu-miR-	FL
  	29a/rno-miR-29a
  	hsa-miR-34b/mmu-miR-	FL
  	34b-3p
  	hsa-miR-32*	FL
  	hsa-miR-29b/mmu-miR-	FL
  	29b/rno-miR-29b
  	hsa-miR-649	FL
  	hsa-miR-576-3p	FL
  	hsa-miR-302a/mmu-	FL
  	miR-302a
  	hsa-miR-365/mmu-miR-	FL
  	365/rno-miR-365
  	hsa-miR-148a/mmu-	FL
  	miR-148a
  	hsa-miR-146b-5p/mmu-	FL
  	miR-146b/rno-miR-146b
  	hsa-miR-505/rno-miR-	FL
  	505
  	hsa-miR-33a/mmu-miR-	FL
  	33/rno-miR-33
  	hsa-miR-455-3p	FL
  	hsa-miR-374b/mmu-	FL
  	miR-374/rno-miR-374
  	hsa-miR-214/mmu-miR-	FL
  	214/rno-miR-214
  	hsa-miR-138-1*/mmu-	FL
  	miR-138*/rno-miR-138*
  	hsa-miR-140-3p/mmu-	FL
  	miR-140*/rno-miR-140*
  	hsa-miR-212/mmu-miR-	FL
  	212/rno-miR-212
  	hsa-miR-29c/mmu-miR-	FL
  	29c/rno-miR-29c
  	hsa-miR-888*	FL
  	hsa-miR-222/mmu-miR-	FL
  	222/rno-miR-222
  	hsa-miR-152/mmu-miR-	FL
  	152/rno-miR-152
  	hsa-miR-183*/mmu-	FL
  	miR-183*
  	hsa-miR-768-5p	FL
  	hsa-miR-107/mmu-miR-	FL
  	107/rno-miR-107
  	hsa-miR-574-5p/mmu-	FL
  	miR-574-5p
  	hsa-miR-154/mmu-miR-	FL
  	154/rno-miR-154
  	hsa-miR-620	FL
  	hsa-miR-886-5p	FL
  	hsa-miR-208a/mmu-	FL
  	miR-208a/rno-miR-208
  	hsa-miR-374b*	FL
  	hsa-miR-525-5p	FL
  	hsa-miR-363/mmu-miR-	FL
  	363/rno-miR-363
  	hsa-miR-99b/mmu-miR-	FL
  	99b/rno-miR-99b
  	hsa-miR-148b/mmu-	FL
  	miR-148b/rno-miR-
  	148b-3p
  	kshv-miR-K12-6-5p	FL
  	hsa-miR-125b-1*/mmu-	FL
  	miR-125b-3p/rno-miR-
  	125b-3p
  	hsa-miR-526b	FL
  	hsa-miR-629	FL
  	hsa-miR-617	FL
  	hsa-miR-124/mmu-miR-	FL
  	124/rno-miR-124
  	hsa-miR-493	FL
  	hsa-miR-24-1*/mmu-	FL
  	miR-24-1*/rno-miR-
  	24-1*
  	hsa-miR-200b*/mmu-	FL
  	miR-200b*
  	hsa-miR-484/mmu-	FL
  	miR-484/rno-miR-484
  	hsa-miR-483-3p	FL
  	hsa-miR-516b	FL
  	hsa-miR-125b-2*/rno-	FL
  	miR-125b*
  	hsa-miR-490-3p/mmu-	FL
  	miR-490
  	hsa-miR-140-5p/mmu-	FL
  	miR-140/rno-miR-140
  	hsa-miR-877/mmu-	FL
  	miR-877/rno-miR-877
  	hsa-miR-381/mmu-	FL
  	miR-381/rno-miR-381
  	hsa-miR-193b*	FL
  	hsa-miR-635	FL
  	hsa-miR-542-3p/mmu-	FL
  	miR-542-3p/rno-miR-
  	542-3p
  	hsa-miR-181a-2*	FL
  	hsa-miR-32/mmu-	FL
  	miR-32/rno-miR-32
  	hsa-miR-105	FL
  	hsa-miR-488	FL
  	hsa-miR-505*	FL
  	ebv-miR-BART16	FL
  	hsa-miR-891a	FL
  	hsa-miR-221/mmu-	FL
  	miR-221/rno-miR-221
  	hsa-miR-7/mmu-miR-	FL
  	7a/rno-miR-7a
  	hsa-miR-299-3p	FL
  	hsa-miR-575	FL
  	hsa-miR-585	FL
  	hsa-miR-30e*/mmu-	FL
  	miR-30e*/rno-miR-
  	30e*
  	hcmv-miR-US25-1*	FL
  	hsa-miR-708/mmu-	FL
  	miR-708/rno-miR-708
  	hsv1-miR-H1	FL
  	hsa-let-7g/mmu-let-7g	FL
  	hsa-miR-146a/mmu-	FL
  	miR-146a/rno-miR-
  	146a
  	ebv-miR-BART8*	FL
  	hsa-miR-106b*/mmu-	FL
  	miR-106b*/rno-miR-
  	106b*
  	hsa-miR-601	FL
  	hsa-miR-553	FL
  	hsa-miR-518b	FL
  	hsa-miR-548d-5p	FL
  	hsa-miR-382/mmu-	FL
  	miR-382/rno-miR-382
  	hsa-miR-630	FL
  	hsa-miR-144*	FL
  	hsa-miR-519d	FL
  	mghv-miR-M1-3	FL
  	hsa-miR-497/mmu-	FL
  	miR-497/rno-miR-497
  	hsa-miR-524-5p	FL
  	hsa-miR-500*	FL
  	hsa-miR-920	FL
  	hsa-miR-297/mmu-	FL
  	miR-297a
  	hsa-miR-509-3-5p	FL
  	hsa-miR-340*/mmu-	FL
  	miR-340-3p/rno-miR-
  	340-3p
  	hsa-miR-99b*/mmu-	FL
  	miR-99b*/rno-miR-
  	99b*
  	hsa-miR-887	FL
  	hsa-miR-331-3p/mmu-	FL
  	miR-331-3p/rno-miR-
  	331
  	hsa-miR-206/mmu-	FL
  	miR-206/rno-miR-206
  	hsa-miR-377*	FL
  	mghv-miR-M1-8	FL
  	hsa-miR-513a-3p	FL
  	hsa-miR-146b-3p	FL
  	hsa-miR-155*	FL
  	hsa-miR-574-3p/mmu-	FL
  	miR-574-3p
  	hsa-miR-615-3p/mmu-	FL
  	miR-615-3p
  	hsa-miR-28-5p/mmu-	FL
  	miR-28/rno-miR-28
  	hsa-miR-934	FL
  	hsa-miR-151-5p/mmu-	FL
  	miR-151-5p/rno-miR-
  	151
  	hsa-miR-885-5p	FL
  	hsa-miR-409-5p/mmu-	FL
  	miR-409-5p/rno-miR-
  	409-5p
  	hsa-let-7d*/mmu-let-	FL
  	7d*/rno-let-7d*
  	hsa-miR-155	FL
  	hsa-let-7b*/mmu-let-	FL
  	7b*/rno-let-7b*
  	hsa-miR-7-2*	FL
  	hsa-miR-221*	FL
  	hsa-miR-9*/mmu-miR-	FL
  	9*/rno-miR-9*
  	hsa-miR-122*	FL
  	hsa-miR-130b/mmu-	FL
  	miR-130b/rno-miR-
  	130b
  	hsa-miR-183/mmu-	FL
  	miR-183/rno-miR-183
  	hsa-miR-92a/mmu-	FL
  	miR-92a/rno-miR-92a
  	hsa-miR-890	FL
  	hsa-miR-938	FL
  	kshv-miR-K12-7	FL
  	hsa-miR-629*	FL
  	hsa-miR-922	FL
  	kshv-miR-K12-5	FL
  	hsa-miR-197/mmu-	FL
  	miR-197
  	hsa-miR-552	FL
  	hsa-miR-151-3p	FL
  	hsa-miR-194/mmu-	FL
  	miR-194/rno-miR-194
  	hsa-miR-218-2*/mmu-	FL
  	miR-218-2*/rno-miR-
  	218*
  	hsa-miR-181b/mmu-	FL
  	miR-181b/rno-miR-
  	181b
  	ebv-miR-BART18-3p	FL
  	hsa-miR-34c-5p/mmu-	FL
  	miR-34c/rno-miR-34c
  	hsa-miR-622	FL
  	hsa-miR-514	FL
  	hsa-miR-657	FL
  	hsa-miR-518a-3p	FL
  	hsa-miR-647	FL
  	hsa-miR-22*/mmu-	FL
  	miR-22*/rno-miR-22*
  	hsa-miR-196a*/mmu-	FL
  	miR-196a*/rno-miR-
  	196a*
  	kshv-miR-K12-1	FL
  	hsa-miR-425/mmu-	FL
  	miR-425/rno-miR-425
  	hsa-miR-361-3p	FL
  	hsa-miR-220b	FL
  	hsa-miR-744/mmu-	FL
  	miR-744
  	hsa-miR-551a	FL
  	hsa-miR-301a/mmu-	FL
  	miR-301a/rno-miR-
  	301a
  	hsa-miR-92b/mmu-	FL
  	miR-92b/rno-miR-92b
  	hsa-miR-487b/mmu-	FL
  	miR-487b/rno-miR-
  	487b
  	hsa-miR-363*/rno-miR-	FL
  	363*
  	hsa-miR-337-3p	FL
  	hsa-miR-636	FL
  	hsa-miR-600	FL
  	hsa-miR-138/mmu-	FL
  	miR-138/rno-miR-138
  	hsa-miR-96/mmu-miR-	FL
  	96/rno-miR-96
  	hsa-miR-20b*	FL
  	hsa-miR-342-5p/mmu-	FL
  	miR-342-5p/rno-miR-
  	342-5p
  	hsa-miR-215	FL
  	hsa-miR-129*	FL

• TABLE 27
  Predictor microRNAs that distinguish Burkitt lymphoma
  from Hodgkin's lymphoma

  		Higher
  	BL vs HL	in
  	hsa-miR-19b/mmu-miR-	BL
  	19b/rno-miR-19b
  	hsa-miR-19a/mmu-miR-	BL
  	19a/rno-miR-19a
  	hsa-miR-17/mmu-miR-	BL
  	17/rno-miR-17-5p/rno-
  	miR-17
  	hsa-miR-106a	BL
  	hsa-miR-20a/mmu-miR-	BL
  	20a/rno-miR-20a
  	hsa-miR-106b/mmu-miR-	BL
  	106b/rno-miR-106b
  	hsa-miR-30c/mmu-miR-	BL
  	30c/rno-miR-30c
  	hsa-miR-551b/mmu-miR-	HL
  	551b/rno-miR-551b
  	hsa-miR-921	HL
  	ebv-miR-BART2-3p	HL
  	hsa-miR-32*	HL
  	hsa-miR-494/mmu-miR-	HL
  	494/rno-miR-494
  	hsa-miR-29c/mmu-miR-	HL
  	29c/rno-miR-29c
  	hsa-miR-923	HL
  	hsa-miR-199b-5p	HL
  	hsa-miR-148a/mmu-miR-	HL
  	148a
  	hsa-miR-130a/mmu-miR-	HL
  	130a/rno-miR-130a
  	hsa-miR-154/mmu-miR-	HL
  	154/rno-miR-154
  	hsa-miR-151-5p/mmu-	HL
  	miR-151-5p/rno-miR-151
  	hsa-miR-28-5p/mmu-	HL
  	miR-28/rno-miR-28
  	hsa-miR-365/mmu-miR-	HL
  	365/rno-miR-365
  	hsa-miR-602	HL
  	hsa-miR-222/mmu-miR-	HL
  	222/rno-miR-222
  	hsa-miR-214/mmu-miR-	HL
  	214/rno-miR-214
  	hsa-miR-144*	HL
  	hsa-miR-107/mmu-miR-	HL
  	107/rno-miR-107
  	hsa-miR-497/mmu-miR-	HL
  	497/rno-miR-497
  	hsa-let-7g/mmu-let-7g	HL
  	hsa-miR-146a/mmu-miR-	HL
  	146a/rno-miR-146a
  	hsa-miR-186/mmu-miR-	HL
  	186/rno-miR-186
  	hsa-miR-886-5p	HL
  	hsa-miR-152/mmu-miR-	HL
  	152/rno-miR-152
  	hsa-miR-29a*/mmu-miR-	HL
  	29a*/rno-miR-29a*
  	hsa-miR-140-5p/mmu-	HL
  	miR-140/rno-miR-140
  	hsa-miR-532-5p/mmu-	HL
  	miR-532-5p/rno-miR-
  	532-5p
  	hsa-miR-145*/mmu-miR-	HL
  	145*
  	hsa-miR-515-5p	HL
  	hsa-miR-153/mmu-miR-	HL
  	153/rno-miR-153
  	hsa-miR-513a-5p	HL
  	hsa-miR-516a-5p	HL
  	hsa-miR-660	HL
  	hsa-miR-29c*/mmu-miR-	HL
  	29c*/rno-miR-29c*
  	hsa-miR-505/rno-miR-	HL
  	505
  	hsa-miR-455-3p	HL
  	hsa-miR-519e*	HL
  	hsa-miR-502-3p	HL
  	hsa-miR-922	HL
  	hsa-miR-524-5p	HL
  	hsa-miR-483-5p	HL
  	hsa-miR-708/mmu-miR-	HL
  	708/rno-miR-708
  	hsa-miR-498	HL
  	ebv-miR-BART19-3p	HL
  	hsa-miR-149/mmu-miR-	HL
  	149
  	hsa-miR-574-3p/mmu-	HL
  	miR-574-3p
  	hsa-miR-659	HL
  	hsa-miR-331-3p/mmu-	HL
  	miR-331-3p/rno-miR-331
  	hsa-miR-105	HL
  	hsa-miR-128/mmu-miR-	HL
  	128/rno-miR-128
  	hsa-miR-200b*/mmu-	HL
  	miR-200b*
  	hsa-miR-381/mmu-miR-	HL
  	381/rno-miR-381
  	hsa-miR-766	HL
  	hsa-miR-557	HL
  	ebv-miR-BART16	HL
  	hsa-miR-488	HL
  	hsa-miR-516b	HL
  	mghv-miR-M1-2	HL
  	hsa-miR-891a	HL
  	hsa-miR-221/mmu-miR-	HL
  	221/rno-miR-221
  	hsa-miR-146b-3p	HL
  	hsa-miR-526b	HL
  	mghv-miR-M1-3	HL
  	hsa-miR-505*	HL
  	hsv1-miR-H1	HL
  	hcmv-miR-UL70-3p	HL
  	hsa-miR-24-2*/mmu-	HL
  	miR-24-2*/rno-miR-24-
  	2*
  	hsa-miR-617	HL
  	hsa-miR-194/mmu-miR-	HL
  	194/rno-miR-194
  	hsa-miR-934	HL
  	hsa-miR-220c	HL
  	hsa-miR-548d-5p	HL
  	hsa-miR-937	HL
  	ebv-miR-BART13	HL
  	hsa-miR-7/mmu-miR-	HL
  	7a/rno-miR-7a
  	hsa-miR-210/mmu-miR-	HL
  	210/rno-miR-210
  	hsa-miR-490-3p/mmu-	HL
  	miR-490
  	hsa-miR-221*	HL
  	hsa-miR-92a/mmu-miR-	HL
  	92a/rno-miR-92a
  	hsa-miR-183*/mmu-miR-	HL
  	183*
  	hsa-miR-513a-3p	HL
  	hsa-miR-575	HL
  	hsa-miR-595	HL
  	hsa-miR-920	HL
  	hsa-miR-483-3p	HL
  	hsa-miR-330-5p/mmu-	HL
  	miR-330/rno-miR-330
  	hsa-miR-525-5p	HL
  	hsa-miR-99b/mmu-miR-	HL
  	99b/rno-miR-99b
  	hsa-miR-509-3p	HL
  	hsa-miR-151-3p	HL
  	ebv-miR-BHRF1-1	HL
  	hsa-miR-630	HL
  	mghv-miR-M1-7-3p	HL
  	hsa-miR-328/mmu-miR-	HL
  	328/rno-miR-328
  	hsa-miR-452	HL
  	hsa-miR-635	HL
  	ebv-miR-BART5	HL
  	hsa-miR-373*	HL
  	hsa-miR-96/mmu-miR-	HL
  	96/rno-miR-96
  	hsa-miR-382/mmu-miR-	HL
  	382/rno-miR-382
  	hsa-miR-155	HL
  	hsa-miR-197/mmu-miR-	HL
  	197
  	kshv-miR-K12-6-5p	HL
  	hcmv-miR-UL112	HL
  	hsa-miR-551b*	HL
  	hsa-miR-877/mmu-miR-	HL
  	877/rno-miR-877
  	hsa-miR-589	HL
  	hsa-miR-936	HL
  	hsa-miR-34c-5p/mmu-	HL
  	miR-34c/rno-miR-34c
  	hsa-miR-885-5p	HL
  	ebv-miR-BART6-3p	HL
  	hsa-miR-585	HL
  	hsa-miR-302c*	HL
  	hsa-miR-196a*/mmu-	HL
  	miR-196a*/rno-miR-
  	196a*
  	hsa-miR-195*	HL
  	hsa-miR-17*/rno-miR-17-	HL
  	3p
  	hsa-miR-296-5p/mmu-	HL
  	miR-296-5p/rno-miR-
  	296*
  	hsa-miR-550*	HL
  	ebv-miR-BHRF1-3	HL
  	hsa-miR-296-3p/mmu-	HL
  	miR-296-3p/rno-miR-296
  	hsa-miR-526b*	HL
  	hsa-miR-548b-3p	HL
  	hsa-miR-652/mmu-miR-	HL
  	652/rno-miR-652
  	hsa-miR-297/mmu-miR-	HL
  	297a
  	hsa-miR-553	HL
  	hsa-miR-194*	HL
  	hsa-miR-23a*/rno-miR-	HL
  	23a*
  	hsa-miR-130b/mmu-miR-	HL
  	130b/rno-miR-130b
  	hsa-miR-586	HL
  	hsa-miR-137/mmu-miR-	HL
  	137/rno-miR-137
  	hsa-miR-610	HL
  	mghv-miR-M1-8	HL
  	hsa-miR-193b*	HL
  	hsa-miR-519d	HL
  	hsa-miR-125b-1*/mmu-	HL
  	miR-125b-3p/rno-miR-
  	125b-3p
  	hsa-miR-744/mmu-miR-	HL
  	744
  	hsa-miR-138/mmu-miR-	HL
  	138/rno-miR-138
  	hsa-miR-21*	HL
  	hsa-miR-576-5p	HL
  	hsa-miR-125a-3p/mmu-	HL
  	miR-125a-3p/rno-miR-
  	125a-3p
  	mghv-miR-M1-6	HL
  	hsa-miR-425/mmu-miR-	HL
  	425/rno-miR-425
  	hsa-miR-10a*/mmu-miR-	HL
  	10a*/rno-miR-10a-3p
  	hsa-miR-215	HL
  	hsa-miR-345	HL
  	hsa-miR-887	HL
  	hsa-miR-7-2*	HL
  	hsa-miR-122*	HL
  	hsa-miR-363*/rno-miR-	HL
  	363*
  	hsa-miR-22*/mmu-miR-	HL
  	22*/rno-miR-22*
  	hsa-miR-542-5p/mmu-	HL
  	miR-542-5p/rno-miR-
  	542-5p
  	hsa-miR-92b*	HL
  	hsa-miR-526a/hsa-	HL
  	miR-520c-5p/hsa-
  	miR-518d-5p
  	kshv-miR-K12-5	HL
  	hsa-miR-	HL
  	340*/mmu-miR-
  	340-3p/rno-miR-
  	340-3p
  	hsa-let-7d*/mmu-	HL
  	let-7d*/rno-let-7d*
  	hsa-miR-92b/mmu-	HL
  	miR-92b/rno-miR-
  	92b
  	hsa-miR-518b	HL
  	hiv1-miR-H1	HL
  	hsa-miR-623	HL
  	hsa-miR-645	HL
  	hsa-miR-601	HL
  	hsa-miR-519e	HL
  	hsa-miR-650	HL
  	hsa-miR-361-3p	HL
  	hsa-miR-	HL
  	150*/mmu-miR-
  	150*
  	hsa-miR-	HL
  	425*/mmu-miR-
  	425*
  	hsa-miR-	HL
  	135a*/mmu-miR-
  	135a*
  	hsa-miR-518a-3p	HL
  	hsa-miR-612	HL
  	hsa-miR-212/mmu-	HL
  	miR-212/rno-miR-
  	212
  	hsa-miR-125b-	HL
  	2*/rno-miR-125b*
  	hsa-miR-500	HL
  	hsa-miR-663	HL
  	hsa-miR-647	HL
  	hsa-miR-502-5p	HL
  	ebv-miR-BART18-	HL
  	3p
  	hsa-miR-	HL
  	99b*/mmu-miR-
  	99b*/rno-miR-99b*
  	ebv-miR-BART7*	HL
  	hsa-miR-500*	HL
  	hsa-miR-	HL
  	124*/mmu-miR-
  	124*/rno-miR-124*
  	hsa-miR-206/mmu-	HL
  	miR-206/rno-miR-
  	206
  	hsa-miR-615-	HL
  	3p/mmu-miR-615-
  	3p
  	hsa-miR-30c-	HL
  	1*/mmu-miR-30c-
  	1*/rno-miR-30c-1*
  	hsa-miR-331-	HL
  	5p/mmu-miR-331-
  	5p
  	hcmv-miR-US25-1*	HL
  	hsa-miR-326/mmu-	HL
  	miR-326/rno-miR-
  	326
  	hsa-miR-	HL
  	181b/mmu-miR-
  	181b/rno-miR-181b
  	hsa-miR-376a*	HL
  	hsa-miR-433/mmu-	HL
  	miR-433/rno-miR-
  	433
  	hsa-miR-640	HL
  	hsa-miR-938	HL
  	hsa-miR-508-5p	HL
  	hsa-miR-484/mmu-	HL
  	miR-484/rno-miR-
  	484
  	hsa-miR-	HL
  	106b*/mmu-miR-
  	106b*/rno-miR-
  	106b*
  	hsa-miR-600	HL
  	hsa-let-7b*/mmu-	HL
  	let-7b*/rno-let-7b*
  	hsa-miR-20b*	HL
  	hsa-miR-622	HL
  	hsa-miR-657	HL
  	hsa-miR-183/mmu-	HL
  	miR-183/rno-miR-
  	183
  	hsa-miR-409-	HL
  	5p/mmu-miR-409-
  	5p/rno-miR-409-5p
  	ebv-miR-BART20-	HL
  	3p
  	ebv-miR-BART8*	HL
  	hsa-miR-129*	HL
  	hsa-miR-629	HL
  	hsa-miR-890	HL
  	hsa-miR-208a/mmu-	HL
  	miR-208a/rno-miR-
  	208
  	hsa-miR-492	HL
  	hsa-miR-629*	HL
  	hsa-miR-654-5p	HL
  	hsa-miR-584	HL
  	hsa-miR-636	HL
  	hsa-miR-490-5p	HL
  	hsa-miR-337-3p	HL
  	hsa-miR-9*/mmu-	HL
  	miR-9*/rno-miR-9*
  	ebv-miR-BART9*	HL
  	hsa-miR-509-3-5p	HL
  	hsa-miR-342-	HL
  	5p/mmu-miR-342-
  	5p/rno-miR-342-5p
  	hsa-miR-514	HL
  	hsa-miR-377*	HL
  	kshv-miR-K12-1	HL
  	hsa-miR-124/mmu-	HL
  	miR-124/rno-miR-
  	124
  	hsa-miR-542-	HL
  	3p/mmu-miR-542-
  	3p/rno-miR-542-3p
  	hsa-miR-220b	HL
  	hsa-miR-299-3p	HL
  	hsa-miR-181a-2*	HL
  	hsa-miR-202	HL
  	hsa-miR-	HL
  	487b/mmu-miR-
  	487b/rno-miR-487b
  	hsa-miR-551a	HL
  	hsa-miR-338-	HL
  	5p/mmu-miR-338-
  	5p/rno-miR-338*
  	hsa-miR-552	HL

• TABLE 28
  Predictor microRNAs that distinguish chronic lymphocytic leukemia
  from follicular lymphoma

  		Higher
  	CLL vs FL	in
  	hsa-miR-331-5p/mmu-	CLL
  	miR-331-5p
  	hsa-miR-144/mmu-miR-	CLL
  	144/rno-miR-144
  	hsa-miR-150/mmu-miR-	CLL
  	150/rno-miR-150
  	hsa-miR-140-5p/mmu-	CLL
  	miR-140/rno-miR-140
  	hsa-miR-335/mmu-miR-	CLL
  	335-5p/rno-miR-335
  	hsa-miR-186/mmu-miR-	CLL
  	186/rno-miR-186
  	hsa-miR-486-5p/mmu-	CLL
  	miR-486
  	hsa-miR-154/mmu-miR-	CLL
  	154/rno-miR-154
  	hsa-miR-223/mmu-miR-	CLL
  	223/rno-miR-223
  	hsa-miR-299-5p/mmu-	CLL
  	miR-299*/rno-miR-299
  	hsa-let-7g/mmu-let-7g	CLL
  	hsa-miR-32/mmu-miR-	CLL
  	32/rno-miR-32
  	hsa-miR-30e*/mmu-miR-	CLL
  	30e*/rno-miR-30e*
  	hsa-miR-147	CLL
  	hsa-miR-20b/mmu-miR-	CLL
  	20b/rno-miR-20b-5p
  	hsa-miR-101/mmu-miR-	CLL
  	101a/rno-miR-101a
  	hsa-let-7f/mmu-let-7f/rno-	CLL
  	let-7f
  	hsa-miR-30e/mmu-miR-	CLL
  	30e/rno-miR-30e
  	hsa-miR-668/mmu-miR-	CLL
  	668
  	hsa-miR-768-5p	CLL
  	hsa-miR-19a/mmu-miR-	CLL
  	19a/rno-miR-19a
  	hsa-miR-199a-5p/mmu-	CLL
  	miR-199a-5p/rno-miR-
  	199a-5p
  	hsa-miR-638	CLL
  	hsa-miR-196a*/mmu-miR-	CLL
  	196a*/rno-miR-196a*
  	hsa-miR-19b/mmu-miR-	CLL
  	19b/rno-miR-19b
  	hsa-miR-30d/mmu-miR-	CLL
  	30d/rno-miR-30d
  	hsa-miR-363/mmu-miR-	CLL
  	363/rno-miR-363
  	hsa-miR-374a	CLL
  	hsa-miR-140-3p/mmu-	CLL
  	miR-140*/rno-miR-140*
  	hsa-miR-185/mmu-miR-	CLL
  	185/rno-miR-185
  	hsa-miR-106b/mmu-miR-	CLL
  	106b/rno-miR-106b
  	hsa-miR-106a	CLL
  	hsa-miR-191/mmu-miR-	CLL
  	191/rno-miR-191
  	hsa-miR-17/mmu-miR-	CLL
  	17/rno-miR-17-5p/rno-
  	miR-17
  	hsa-let-7i/mmu-let-7i/rno-	CLL
  	let-7i
  	hsa-miR-20a/mmu-miR-	CLL
  	20a/rno-miR-20a
  	hsa-miR-142-5p/mmu-	CLL
  	miR-142-5p/rno-miR-142-
  	5p
  	hsa-miR-768-3p	CLL
  	hsa-miR-30b/mmu-miR-	CLL
  	30b/rno-miR-30b-5p
  	hsa-miR-891a	CLL
  	hsa-miR-24-1*/mmu-miR-	CLL
  	24-1*/rno-miR-24-1*
  	hsa-miR-29c/mmu-miR-	CLL
  	29c/rno-miR-29c
  	hsa-miR-28-5p/mmu-miR-	CLL
  	28/rno-miR-28
  	hsa-miR-30a/mmu-miR-	CLL
  	30a/rno-miR-30a
  	hsa-miR-155	CLL
  	hsa-miR-361-5p/mmu-	CLL
  	miR-361/rno-miR-361
  	hsa-miR-15a/mmu-miR-	CLL
  	15a
  	hsa-miR-26a/mmu-miR-	CLL
  	26a/rno-miR-26a
  	hsa-miR-30c/mmu-miR-	CLL
  	30c/rno-miR-30c
  	hsa-miR-541*	CLL
  	hsa-miR-26b/mmu-miR-	CLL
  	26b/rno-miR-26b
  	hsa-miR-519d	CLL
  	hsa-miR-15b/mmu-miR-	CLL
  	15b/rno-miR-15b
  	hsa-miR-550	CLL
  	hsa-miR-29b/mmu-miR-	CLL
  	29b/rno-miR-29b
  	hsa-miR-29a/mmu-miR-	CLL
  	29a/rno-miR-29a
  	hsa-miR-103/mmu-miR-	CLL
  	103/rno-miR-103
  	hsa-miR-423-3p/mmu-	CLL
  	miR-423-3p/rno-miR-423
  	hsa-miR-549	CLL
  	hsa-miR-107/mmu-miR-	CLL
  	107/rno-miR-107
  	hsa-miR-888*	CLL
  	hsa-miR-801/mmu-miR-	CLL
  	801
  	hsa-miR-149*	FL
  	hsa-miR-634	FL
  	ebv-miR-BART2-3p	FL
  	hsa-miR-921	FL
  	hsa-miR-494/mmu-miR-	FL
  	494/rno-miR-494
  	hsa-miR-933	FL
  	mghv-miR-M1-4	FL
  	hsa-let-7e/mmu-let-7e/rno-	FL
  	let-7e
  	hsa-miR-939	FL
  	hsa-miR-518c*	FL
  	hsa-miR-32*	FL
  	hsa-miR-491-3p	FL
  	hsa-miR-185/mmu-miR-	FL
  	185/rno-miR-185
  	hsa-miR-765	FL
  	ebv-miR-BART17-5p	FL
  	hsa-miR-576-3p	FL
  	hsa-miR-658	FL
  	hsa-miR-503	FL
  	hsa-miR-30b*	FL
  	hsa-miR-302a/mmu-miR-	FL
  	302a
  	hsa-miR-628-3p	FL
  	hsa-miR-642	FL
  	hsa-miR-620	FL
  	hsa-miR-99a/mmu-	FL
  	miR-99a/rno-miR-
  	99a
  	hsa-miR-371-5p	FL
  	hsa-miR-452	FL
  	hsa-miR-126*/mmu-	FL
  	miR-126-5p/rno-
  	miR-126*
  	hsa-miR-298	FL
  	hsa-miR-193a-5p	FL
  	hsa-miR-583	FL
  	hsa-miR-143/mmu-	FL
  	miR-143/rno-miR-
  	143
  	hsa-miR-665	FL
  	hsa-miR-505/rno-	FL
  	miR-505
  	hsa-miR-199b-5p	FL
  	hsa-miR-28-3p/rno-	FL
  	miR-28*
  	hsa-miR-422a	FL
  	hsa-miR-515-5p	FL
  	hsa-miR-455-3p	FL
  	hsa-miR-10a/mmu-	FL
  	miR-10a/rno-miR-
  	10a-5p
  	hsa-miR-300	FL
  	ebv-miR-BART5	FL
  	hsa-miR-10b/mmu-	FL
  	miR-10b/rno-miR-
  	10b
  	hsa-miR-212/mmu-	FL
  	miR-212/rno-miR-
  	212
  	hsa-miR-145/mmu-	FL
  	miR-145/rno-miR-
  	145
  	hsa-miR-187*	FL
  	ebv-miR-BHRF1-1	FL
  	ebv-miR-BHRF1-2	FL
  	hsa-miR-126/mmu-	FL
  	miR-126-3p/rno-
  	miR-126
  	hsa-miR-	FL
  	130b*/mmu-miR-
  	130b*
  	hsa-miR-326/mmu-	FL
  	miR-326/rno-miR-
  	326
  	mghv-miR-M1-2	FL
  	kshv-miR-K12-6-3p	FL
  	hsa-miR-516b	FL
  	hsa-miR-519e*	FL
  	mghv-miR-M1-7-3p	FL
  	hsa-miR-629	FL
  	hsa-miR-24-2*/mmu-	FL
  	miR-24-2*/rno-miR-
  	24-2*
  	hsa-miR-943	FL
  	hsa-miR-124/mmu-	FL
  	miR-124/rno-miR-
  	124
  	hsa-miR-365/mmu-	FL
  	miR-365/rno-miR-
  	365
  	hsa-miR-493	FL
  	hsa-miR-29c*/mmu-	FL
  	miR-29c*/rno-miR-
  	29c*
  	hsa-miR-602	FL
  	hsa-miR-484/mmu-	FL
  	miR-484/rno-miR-
  	484
  	hsa-miR-483-3p	FL
  	hsa-miR-125b-	FL
  	2*/rno-miR-125b*
  	hsa-miR-675	FL
  	mghv-miR-M1-7-5p	FL
  	hsa-miR-152/mmu-	FL
  	miR-152/rno-miR-
  	152
  	hsa-miR-27a*/mmu-	FL
  	miR-27a*/rno-miR-
  	27a*
  	hsa-miR-542-	FL
  	3p/mmu-miR-542-
  	3p/rno-miR-542-3p
  	hsa-miR-100/mmu-	FL
  	miR-100/rno-miR-
  	100
  	hsa-miR-208a/mmu-	FL
  	miR-208a/rno-miR-
  	208
  	hsa-miR-766	FL
  	hsa-miR-637	FL
  	hsa-miR-519c-	FL
  	5p/hsa-miR-519b-
  	5p/hsa-miR-
  	523*/hsa-miR-
  	518e*/hsa-miR-
  	522*/hsa-miR-519a*
  	hsa-miR-409-	FL
  	5p/mmu-miR-409-
  	5p/rno-miR-409-5p
  	hsa-miR-199a-	FL
  	3p/hsa-miR-199b-
  	3p/mmu-miR-199a-
  	3p/mmu-miR-
  	199b/rno-miR-199a-
  	3p
  	hsa-miR-	FL
  	106b*/mmu-miR-
  	106b*/rno-miR-
  	106b*
  	hsa-miR-130a/mmu-	FL
  	miR-130a/rno-miR-
  	130a
  	hsa-miR-645	FL
  	hsa-miR-548d-5p	FL
  	hsa-miR-671-	FL
  	5p/mmu-miR-671-5p
  	hsa-miR-574-	FL
  	3p/mmu-miR-574-3p
  	hsa-miR-125b/mmu-	FL
  	miR-125b-5p/rno-
  	miR-125b-5p
  	hsa-miR-500*	FL
  	hsa-miR-425/mmu-	FL
  	miR-425/rno-miR-
  	425
  	ebv-miR-BART8*	FL
  	hsa-miR-377*	FL
  	hsa-miR-513a-3p	FL
  	hsa-miR-181a-2*	FL
  	hsa-miR-938	FL
  	hsa-miR-155*	FL
  	hsa-miR-218-	FL
  	2*/mmu-miR-218-
  	2*/rno-miR-218*
  	hsa-miR-198	FL
  	hsa-miR-151-3p	FL
  	hsa-miR-299-3p	FL
  	hcmv-miR-US25-1*	FL
  	hsa-miR-708/mmu-	FL
  	miR-708/rno-miR-
  	708
  	hsa-miR-659	FL
  	hsa-miR-600	FL
  	hsa-miR-601	FL
  	hsa-miR-296-	FL
  	3p/mmu-miR-296-
  	3p/rno-miR-296
  	hsa-let-7b*/mmu-let-	FL
  	7b*/rno-let-7b*
  	ebv-miR-BART6-3p	FL
  	hsa-miR-7-2*	FL
  	hsa-miR-9*/mmu-	FL
  	miR-9*/rno-miR-9*
  	hsa-miR-509-3-5p	FL
  	hsa-miR-518b	FL
  	hsa-miR-183*/mmu-	FL
  	miR-183*
  	hsa-miR-125b-	FL
  	1*/mmu-miR-125b-
  	3p/rno-miR-125b-3p
  	hsa-miR-183/mmu-	FL
  	miR-183/rno-miR-
  	183
  	hsa-miR-890	FL
  	hsa-miR-153/mmu-	FL
  	miR-153/rno-miR-
  	153
  	hsa-miR-874/mmu-	FL
  	miR-874/rno-miR-
  	874
  	hsa-miR-220c	FL
  	hsa-miR-99b*/mmu-	FL
  	miR-99b*/rno-miR-
  	99b*
  	hsa-miR-193b*	FL
  	hsa-miR-629*	FL
  	hcmv-miR-UL148D	FL
  	ebv-miR-BART7*	FL
  	hsa-miR-99b/mmu-	FL
  	miR-99b/rno-miR-
  	99b
  	hsa-miR-206/mmu-	FL
  	miR-206/rno-miR-
  	206
  	hsa-miR-381/mmu-	FL
  	miR-381/rno-miR-
  	381
  	hsa-miR-194/mmu-	FL
  	miR-194/rno-miR-
  	194
  	hsa-miR-525-5p	FL
  	hsa-miR-193b	FL
  	hsa-miR-497/mmu-	FL
  	miR-497/rno-miR-
  	497
  	ebv-miR-BART18-	FL
  	3p
  	hsa-miR-424	FL
  	hsa-miR-553	FL
  	hsa-let-7d*/mmu-let-	FL
  	7d*/rno-let-7d*
  	hsa-miR-34c-	FL
  	5p/mmu-miR-
  	34c/rno-miR-34c
  	ebv-miR-BHRF1-3	FL
  	kshv-miR-K12-6-5p	FL
  	hsa-miR-551a	FL
  	hsa-miR-195*	FL
  	hsa-miR-551b*	FL
  	hsa-miR-514	FL
  	hsa-miR-552	FL
  	hsa-miR-122*	FL
  	hsa-miR-92b/mmu-	FL
  	miR-92b/rno-miR-
  	92b
  	hsa-miR-22*/mmu-	FL
  	miR-22*/rno-miR-
  	22*
  	hsa-miR-635	FL
  	kshv-miR-K12-1	FL
  	hsa-miR-483-5p	FL
  	hsa-miR-340*/mmu-	FL
  	miR-340-3p/rno-
  	miR-340-3p
  	hsa-miR-615-	FL
  	3p/mmu-miR-615-3p
  	hsa-miR-505*	FL
  	hsa-miR-622	FL
  	hsa-miR-181b/mmu-	FL
  	miR-181b/rno-miR-
  	181b
  	hsa-miR-886-5p	FL
  	hsa-miR-885-5p	FL
  	hsa-miR-220b	FL
  	hsa-miR-524-5p	FL
  	hsa-miR-382/mmu-	FL
  	miR-382/rno-miR-
  	382
  	hsa-miR-744/mmu-	FL
  	miR-744
  	hsv1-miR-H1	FL
  	hsa-miR-526b	FL
  	hsa-miR-657	FL
  	hsa-miR-130b/mmu-	FL
  	miR-130b/rno-miR-
  	130b
  	hsa-miR-181a/mmu-	FL
  	miR-181a/rno-miR-
  	181a
  	hsa-miR-301a/mmu-	FL
  	miR-301a/rno-miR-
  	301a
  	hsa-miR-490-	FL
  	3p/mmu-miR-490
  	hsa-miR-485-	FL
  	3p/mmu-miR-485*
  	hsa-miR-297/mmu-	FL
  	miR-297a
  	hsa-miR-630	FL
  	hsa-miR-877/mmu-	FL
  	miR-877/rno-miR-
  	877
  	kshv-miR-K12-5	FL
  	hsa-miR-617	FL
  	mghv-miR-M1-3	FL
  	hsa-miR-920	FL
  	hsa-miR-585	FL
  	hsa-miR-374b*	FL
  	hsa-miR-215	FL
  	hsa-miR-342-	FL
  	5p/mmu-miR-342-
  	5p/rno-miR-342-5p
  	hsa-miR-934	FL
  	hsa-miR-575	FL
  	hsa-miR-488	FL
  	ebv-miR-BART16	FL
  	hsa-miR-647	FL
  	hsa-miR-138/mmu-	FL
  	miR-138/rno-miR-
  	138
  	hsa-miR-221*	FL
  	hsa-miR-	FL
  	200b*/mmu-miR-
  	200b*
  	hsa-miR-337-3p	FL
  	hsa-miR-922	FL
  	hsa-miR-197/mmu-	FL
  	miR-197
  	hsa-miR-96/mmu-	FL
  	miR-96/rno-miR-96
  	hsa-miR-518a-3p	FL

• TABLE 29
  Predictor microRNAs that distinguish Burkitt lymphoma
  from Hodgkin's lymphoma

  		Higher
  	CLL vs HL	in
  	hsa-miR-32/mmu-miR-	CLL
  	32/rno-miR-32
  	hsa-miR-30e*/mmu-miR-	CLL
  	30e*/rno-miR-30e*
  	hsa-let-7g/mmu-let-7g	CLL
  	hsa-miR-144/mmu-miR-	CLL
  	144/rno-miR-144
  	hsa-miR-140-5p/mmu-	CLL
  	miR-140/rno-miR-140
  	hsa-miR-19a/mmu-miR-	CLL
  	19a/rno-miR-19a
  	hsa-miR-154/mmu-miR-	CLL
  	154/rno-miR-154
  	hsa-miR-150/mmu-miR-	CLL
  	150/rno-miR-150
  	hsa-miR-28-5p/mmu-	CLL
  	miR-28/rno-miR-28
  	hsa-miR-363/mmu-miR-	CLL
  	363/rno-miR-363
  	hsa-miR-101/mmu-miR-	CLL
  	101a/rno-miR-101a
  	hsa-miR-299-5p/mmu-	CLL
  	miR-299*/rno-miR-299
  	hsa-miR-768-5p	CLL
  	hsa-miR-19b/mmu-miR-	CLL
  	19b/rno-miR-19b
  	hsa-miR-30e/mmu-miR-	CLL
  	30e/rno-miR-30e
  	hsa-miR-20b/mmu-miR-	CLL
  	20b/rno-miR-20b-5p
  	hsa-miR-374a	CLL
  	hsa-let-7f/mmu-let-7f/rno-	CLL
  	let-7f
  	hsa-miR-335/mmu-miR-	CLL
  	335-5p/rno-miR-335
  	hsa-miR-142-5p/mmu-	CLL
  	miR-142-5p/rno-miR-142-
  	5p
  	hsa-miR-486-5p/mmu-	CLL
  	miR-486
  	hsa-miR-33a/mmu-miR-	CLL
  	33/rno-miR-33
  	hsa-miR-30b/mmu-miR-	CLL
  	30b/rno-miR-30b-5p
  	hsa-miR-768-3p	CLL
  	hsa-miR-668/mmu-miR-	CLL
  	668
  	hsa-miR-15b/mmu-miR-	CLL
  	15b/rno-miR-15b
  	hsa-miR-196a*/mmu-	CLL
  	miR-196a*/rno-miR-196a*
  	hsa-miR-140-3p/mmu-	CLL
  	miR-140*/rno-miR-140*
  	hsa-miR-29b/mmu-miR-	CLL
  	29b/rno-miR-29b
  	hsa-miR-29c/mmu-miR-	CLL
  	29c/rno-miR-29c
  	hsa-miR-186/mmu-miR-	CLL
  	186/rno-miR-186
  	hsa-miR-106a	CLL
  	hsa-miR-26a/mmu-miR-	CLL
  	26a/rno-miR-26a
  	hsa-miR-106b/mmu-miR-	CLL
  	106b/rno-miR-106b
  	hsa-miR-17/mmu-miR-	CLL
  	17/rno-miR-17-5p/rno-
  	miR-17
  	hsa-miR-191/mmu-miR-	CLL
  	191/rno-miR-191
  	hsa-miR-20a/mmu-miR-	CLL
  	20a/rno-miR-20a
  	hsa-miR-30c/mmu-miR-	CLL
  	30c/rno-miR-30c
  	hsa-miR-26b/mmu-miR-	CLL
  	26b/rno-miR-26b
  	hsa-miR-147	CLL
  	hsa-miR-15a/mmu-miR-	CLL
  	15a
  	hsa-miR-30d/mmu-miR-	CLL
  	30d/rno-miR-30d
  	hsa-miR-199a-5p/mmu-	CLL
  	miR-199a-5p/rno-miR-
  	199a-5p
  	hsa-miR-29a/mmu-miR-	CLL
  	29a/rno-miR-29a
  	hsa-miR-223/mmu-miR-	CLL
  	223/rno-miR-223
  	hsa-miR-30a/mmu-miR-	CLL
  	30a/rno-miR-30a
  	hsa-miR-16/mmu-miR-	CLL
  	16/rno-miR-16
  	hsa-miR-451/mmu-miR-	CLL
  	451/rno-miR-451
  	hsa-miR-24-1*/mmu-miR-	CLL
  	24-1*/rno-miR-24-1*
  	hsa-miR-550	CLL
  	hsa-miR-342-3p/mmu-	CLL
  	miR-342-3p/rno-miR-342-
  	3p
  	hsa-miR-195/mmu-miR-	CLL
  	195/rno-miR-195
  	hsa-miR-801/mmu-miR-	CLL
  	801
  	hsa-miR-541*	CLL
  	hsa-let-7i/mmu-let-7i/rno-	CLL
  	let-7i
  	hsa-miR-155	CLL
  	hsa-miR-185/mmu-miR-	CLL
  	185/rno-miR-185
  	hsa-miR-891a	CLL
  	hsa-miR-138-1*/mmu-	CLL
  	miR-138*/rno-miR-138*
  	hsa-miR-27b/mmu-miR-	CLL
  	27b/rno-miR-27b
  	hsa-miR-361-5p/mmu-	CLL
  	miR-361/rno-miR-361
  	hsa-miR-129*	CLL
  	hsa-miR-638	CLL
  	hsa-miR-34b/mmu-miR-	CLL
  	34b-3p
  	hsa-miR-107/mmu-miR-	CLL
  	107/rno-miR-107
  	hsa-miR-549	CLL
  	hsa-miR-888*	CLL
  	hsa-miR-423-3p/mmu-	CLL
  	miR-423-3p/rno-miR-423
  	hsa-let-7e/mmu-let-	HL
  	7e/rno-let-7e
  	hsa-miR-125a-5p/mmu-	HL
  	miR-125a-5p/rno-miR-
  	125a-5p
  	hsa-miR-576-3p	HL
  	hsa-miR-513a-5p	HL
  	ebv-miR-BART17-5p	HL
  	hsa-miR-185/mmu-miR-	HL
  	185/rno-miR-185
  	hsa-miR-921	HL
  	hsa-miR-518c*	HL
  	hsa-miR-520d-5p	HL
  	hsa-miR-939	HL
  	hsa-miR-634	HL
  	hsa-miR-491-3p	HL
  	ebv-miR-BART2-3p	HL
  	hsa-miR-30c-2*/mmu-	HL
  	miR-30c-2*/rno-miR-30c-
  	2*
  	hsa-miR-765	HL
  	hsa-miR-923	HL
  	hsa-miR-620	HL
  	hsa-miR-933	HL
  	hsa-miR-143/mmu-miR-	HL
  	143/rno-miR-143
  	hsa-miR-494/mmu-miR-	HL
  	494/rno-miR-494
  	hsa-miR-665	HL
  	hsa-miR-642	HL
  	hsa-miR-126*/mmu-miR-	HL
  	126-5p/rno-miR-126*
  	hsa-miR-658	HL
  	hsa-miR-149*	HL
  	hsa-miR-30b*	HL
  	mghv-miR-M1-4	HL
  	hsa-miR-99a/mmu-miR-	HL
  	99a/rno-miR-99a
  	hsa-miR-193a-5p	HL
  	hsa-miR-498	HL
  	hsa-miR-628-3p	HL
  	hsa-miR-185*	HL
  	hsa-miR-371-5p	HL
  	hsa-miR-199b-5p	HL
  	hsa-miR-126/mmu-miR-	HL
  	126-3p/rno-miR-126
  	hsa-miR-503	HL
  	hsa-miR-10a/mmu-miR-	HL
  	10a/rno-miR-10a-5p
  	hsa-miR-300	HL
  	hsa-miR-583	HL
  	hsa-miR-518a-5p/hsa-	HL
  	miR-527
  	hsa-miR-10b/mmu-miR-	HL
  	10b/rno-miR-10b
  	hsa-miR-145/mmu-	HL
  	miR-145/rno-miR-
  	145
  	hsa-miR-128/mmu-	HL
  	miR-128/rno-miR-
  	128
  	hsa-miR-532-	HL
  	5p/mmu-miR-532-
  	5p/rno-miR-532-5p
  	hsa-miR-143*	HL
  	hsa-miR-28-3p/rno-	HL
  	miR-28*
  	hsa-miR-	HL
  	130b*/mmu-miR-
  	130b*
  	hsa-miR-505/rno-	HL
  	miR-505
  	hsa-miR-25*	HL
  	hsa-miR-574-	HL
  	3p/mmu-miR-574-3p
  	hsa-miR-455-3p	HL
  	kshv-miR-K12-3	HL
  	hsa-miR-516b	HL
  	kshv-miR-K12-8	HL
  	hsa-miR-502-3p	HL
  	kshv-miR-K12-6-3p	HL
  	hsa-miR-129-	HL
  	5p/mmu-miR-129-
  	5p/rno-miR-129
  	hsa-miR-515-5p	HL
  	hsa-miR-199a-	HL
  	3p/hsa-miR-199b-
  	3p/mmu-miR-199a-
  	3p/mmu-miR-
  	199b/rno-miR-199a-
  	3p
  	hsa-miR-149/mmu-	HL
  	miR-149
  	hsa-miR-889	HL
  	hsa-miR-637	HL
  	hsa-miR-600	HL
  	hsa-miR-151-3p	HL
  	hsa-miR-656	HL
  	hsa-miR-497/mmu-	HL
  	miR-497/rno-miR-
  	497
  	hsa-miR-152/mmu-	HL
  	miR-152/rno-miR-
  	152
  	hsa-miR-100/mmu-	HL
  	miR-100/rno-miR-
  	100
  	hsa-miR-425/mmu-	HL
  	miR-425/rno-miR-
  	425
  	hsa-miR-145*/mmu-	HL
  	miR-145*
  	hsa-miR-365/mmu-	HL
  	miR-365/rno-miR-
  	365
  	hsa-miR-422a	HL
  	hcmv-miR-UL70-3p	HL
  	hsa-miR-27a*/mmu-	HL
  	miR-27a*/rno-miR-
  	27a*
  	hsa-miR-194/mmu-	HL
  	miR-194/rno-miR-
  	194
  	hsa-miR-548d-5p	HL
  	hsa-miR-187*	HL
  	hsa-miR-323-	HL
  	3p/mmu-miR-323-
  	3p/rno-miR-323
  	hsa-miR-708/mmu-	HL
  	miR-708/rno-miR-
  	708
  	hsa-miR-29c*/mmu-	HL
  	miR-29c*/rno-miR-
  	29c*
  	hsa-miR-513a-3p	HL
  	hsa-miR-595	HL
  	hsa-miR-483-3p	HL
  	hsa-miR-330-	HL
  	5p/mmu-miR-
  	330/rno-miR-330
  	hsa-miR-519e*	HL
  	hsa-miR-509-3p	HL
  	hsa-miR-328/mmu-	HL
  	miR-328/rno-miR-
  	328
  	hsa-miR-373*	HL
  	hsa-miR-96/mmu-	HL
  	miR-96/rno-miR-96
  	hsa-miR-215	HL
  	hsa-miR-589	HL
  	hsa-miR-34c-	HL
  	5p/mmu-miR-
  	34c/rno-miR-34c
  	hsa-miR-125b/mmu-	HL
  	miR-125b-5p/rno-
  	miR-125b-5p
  	hsa-miR-130a/mmu-	HL
  	miR-130a/rno-miR-
  	130a
  	hsa-miR-519c-	HL
  	5p/hsa-miR-519b-
  	5p/hsa-miR-
  	523*/hsa-miR-
  	518e*/hsa-miR-
  	522*/hsa-miR-519a*
  	mghv-miR-M1-7-5p	HL
  	hsa-miR-516a-5p	HL
  	hsa-miR-424	HL
  	hsa-miR-17*/rno-	HL
  	miR-17-3p
  	hsa-miR-296-	HL
  	5p/mmu-miR-296-
  	5p/rno-miR-296*
  	hsa-miR-550*	HL
  	hsa-miR-210/mmu-	HL
  	miR-210/rno-miR-
  	210
  	hsa-miR-92b/mmu-	HL
  	miR-92b/rno-miR-
  	92b
  	hsa-miR-548b-3p	HL
  	hsa-miR-652/mmu-	HL
  	miR-652/rno-miR-
  	652
  	hsa-miR-138/mmu-	HL
  	miR-138/rno-miR-
  	138
  	hsa-miR-194*	HL
  	hsa-miR-23a*/rno-	HL
  	miR-23a*
  	hsa-miR-153/mmu-	HL
  	miR-153/rno-miR-
  	153
  	hsa-miR-586	HL
  	hsa-miR-137/mmu-	HL
  	miR-137/rno-miR-
  	137
  	hsa-miR-610	HL
  	hsa-miR-381/mmu-	HL
  	miR-381/rno-miR-
  	381
  	hsa-miR-936	HL
  	hsa-miR-744/mmu-	HL
  	miR-744
  	ebv-miR-BART5	HL
  	ebv-miR-BHRF1-1	HL
  	hsa-miR-21*	HL
  	hsa-miR-576-5p	HL
  	mghv-miR-M1-6	HL
  	hsa-miR-193b	HL
  	hsa-miR-10a*/mmu-	HL
  	miR-10a*/rno-miR-
  	10a-3p
  	hsa-miR-524-5p	HL
  	hsa-miR-452	HL
  	hsa-miR-345	HL
  	hsa-miR-7-2*	HL
  	hsa-miR-409-	HL
  	5p/mmu-miR-409-
  	5p/rno-miR-409-5p
  	hsa-miR-557	HL
  	hsa-miR-181a/mmu-	HL
  	miR-181a/rno-miR-
  	181a
  	hsa-miR-22*/mmu-	HL
  	miR-22*/rno-miR-22*
  	hsa-miR-922	HL
  	hsa-miR-92b*	HL
  	hsa-miR-938	HL
  	hsa-miR-526a/hsa-	HL
  	miR-520c-5p/hsa-
  	miR-518d-5p
  	hsa-miR-526b*	HL
  	ebv-miR-BHRF1-2	HL
  	hiv1-miR-H1	HL
  	hsa-miR-623	HL
  	mghv-miR-M1-2	HL
  	mghv-miR-M1-7-3p	HL
  	hsa-miR-519e	HL
  	hsa-miR-650	HL
  	hsa-miR-766	HL
  	hsa-miR-602	HL
  	hsa-miR-425*/mmu-	HL
  	miR-425*
  	hsa-miR-	HL
  	135a*/mmu-miR-
  	135a*	HL
  	hsa-miR-612	HL
  	hsa-miR-212/mmu-	HL
  	miR-212/rno-miR-
  	212
  	hsa-miR-125b-	HL
  	2*/rno-miR-125b*
  	hcmv-miR-UL112	HL
  	hsa-miR-374b*
  	hsa-miR-886-5p	HL
  	hsa-miR-500	HL
  	hsa-miR-502-5p	HL
  	ebv-miR-BART18-	HL
  	3p
  	hsa-miR-198	HL
  	hsa-miR-500*	HL
  	hsa-miR-342-	HL
  	5p/mmu-miR-342-
  	5p/rno-miR-342-5p
  	hsa-miR-124*/mmu-	HL
  	miR-124*/rno-miR-
  	124*
  	hsa-miR-30c-	HL
  	1*/mmu-miR-30c-
  	1*/rno-miR-30c-1*
  	hsa-miR-220c	HL
  	hsa-miR-376a*	HL
  	hsa-miR-640	HL
  	hcmv-miR-UL148D	HL
  	hsa-miR-659	HL
  	hsa-miR-934	HL
  	hsa-miR-125a-	HL
  	3p/mmu-miR-125a-
  	3p/rno-miR-125a-3p
  	hsa-miR-885-5p	HL
  	hsa-miR-24-	HL
  	2*/mmu-miR-24-
  	2*/rno-miR-24-2*
  	hsa-miR-484/mmu-	HL
  	miR-484/rno-miR-
  	484
  	hsa-miR-	HL
  	106b*/mmu-miR-
  	106b*/rno-miR-
  	106b*
  	hsa-miR-505*	HL
  	hsa-let-7b*/mmu-let-	HL
  	7b*/rno-let-7b*
  	hsa-miR-302c*	HL
  	hsa-miR-542-	HL
  	5p/mmu-miR-542-
  	5p/rno-miR-542-5p
  	hsv1-miR-H1	HL
  	mghv-miR-M1-3	HL
  	hsa-miR-183/mmu-	HL
  	miR-183/rno-miR-
  	183
  	hsa-miR-122*	HL
  	hsa-miR-183*/mmu-	HL
  	miR-183*
  	hsa-miR-675	HL
  	hsa-miR-99b/mmu-	HL
  	miR-99b/rno-miR-
  	99b
  	hsa-miR-874/mmu-	HL
  	miR-874/rno-miR-
  	874
  	ebv-miR-BART20-	HL
  	3p
  	hsa-miR-483-5p	HL
  	hsa-miR-671-	HL
  	5p/mmu-miR-671-5p
  	hsa-miR-629	HL
  	hsa-miR-553	HL
  	hsa-let-7d*/mmu-let-	HL
  	7d*/rno-let-7d*
  	hsa-miR-601	HL
  	hsa-miR-645	HL
  	hsa-miR-920	HL
  	hsa-miR-525-5p	HL
  	hsa-miR-221*	HL
  	hsa-miR-890	HL
  	hsa-miR-492	HL
  	hsa-miR-629*	HL
  	hsa-miR-635	HL
  	hsa-miR-130b/mmu-	HL
  	miR-130b/rno-miR-
  	130b
  	hsa-miR-197/mmu-	HL
  	miR-197
  	hsa-miR-654-5p	HL
  	hsa-miR-518b	HL
  	hsa-miR-382/mmu-	HL
  	miR-382/rno-miR-
  	382
  	hsa-miR-584	HL
  	hsa-miR-99b*/mmu-	HL
  	miR-99b*/rno-miR-
  	99b*
  	hsa-miR-630	HL
  	hsa-miR-490-5p	HL
  	hsa-miR-663	HL
  	hsa-miR-337-3p	HL
  	hsa-miR-9*/mmu-	HL
  	miR-9*/rno-miR-9*
  	hsa-miR-202	HL
  	ebv-miR-BART16	HL
  	ebv-miR-BART9*	HL
  	hsa-miR-193b*	HL
  	ebv-miR-BART8*	HL
  	hsa-miR-206/mmu-	HL
  	miR-206/rno-miR-
  	206
  	hcmv-miR-US25-1*	HL
  	hsa-miR-514	HL
  	kshv-miR-K12-6-5p	HL
  	hsa-miR-488	HL
  	hsa-miR-508-5p	HL
  	hsa-miR-551b*	HL
  	hsa-miR-377*	HL
  	ebv-miR-BART6-3p	HL
  	hsa-miR-181b/mmu-	HL
  	miR-181b/rno-miR-
  	181b
  	hsa-miR-526b	HL
  	hsa-miR-622	HL
  	kshv-miR-K12-1	HL
  	hsa-miR-485-	HL
  	3p/mmu-miR-485*
  	hsa-miR-490-	HL
  	3p/mmu-miR-490
  	hsa-miR-125b-	HL
  	1*/mmu-miR-125b-
  	3p/rno-miR-125b-3p
  	hsa-miR-124/mmu-	HL
  	miR-124/rno-miR-
  	124
  	hsa-miR-657	HL
  	ebv-miR-BHRF1-3	HL
  	hsa-miR-542-	HL
  	3p/mmu-miR-542-
  	3p/rno-miR-542-3p
  	kshv-miR-K12-5	HL
  	hsa-miR-943	HL
  	hsa-miR-551a	HL
  	hsa-miR-297/mmu-	HL
  	miR-297a
  	hsa-miR-296-	HL
  	3p/mmu-miR-296-
  	3p/rno-miR-296
  	hsa-miR-617	HL
  	hsa-miR-195*	HL
  	hsa-miR-575	HL
  	hsa-miR-208a/mmu-	HL
  	miR-208a/rno-miR-
  	208
  	hsa-miR-647	HL
  	hsa-miR-509-3-5p	HL
  	hsa-miR-340*/mmu-	HL
  	miR-340-3p/rno-
  	miR-340-3p
  	hsa-miR-220b	HL
  	hsa-miR-	HL
  	200b*/mmu-miR-
  	200b*
  	hsa-miR-585	HL
  	hsa-miR-877/mmu-	HL
  	miR-877/rno-miR-
  	877
  	hsa-miR-326/mmu-	HL
  	miR-326/rno-miR-
  	326
  	ebv-miR-BART7*	HL
  	hsa-miR-615-	HL
  	3p/mmu-miR-615-3p
  	hsa-miR-433/mmu-	HL
  	miR-433/rno-miR-
  	433
  	hsa-miR-338-	HL
  	5p/mmu-miR-338-
  	5p/rno-miR-338*
  	hsa-miR-299-3p	HL
  	hsa-miR-518a-3p	HL
  	hsa-miR-181a-2*	HL
  	hsa-miR-552	HL

• TABLE 30
  Predictor microRNAs that distinguish follicular lymphoma
  from Hodgkin's lymphoma

  		Higher
  	FL vs HL	in
  	hsa-miR-301a/mmu-miR-	FL
  	301a/rno-miR-301a
  	kshv-miR-K12-7	FL
  	hsa-miR-96/mmu-miR-	FL
  	96/rno-miR-96
  	hsa-miR-151-5p/mmu-	FL
  	miR-151-5p/rno-miR-151
  	hsa-miR-28-5p/mmu-miR-	FL
  	28/rno-miR-28
  	hsa-miR-302a/mmu-miR-	FL
  	302a
  	hsa-miR-215	FL
  	hsa-miR-15b/mmu-miR-	FL
  	15b/rno-miR-15b
  	hsa-miR-29b/mmu-miR-	FL
  	29b/rno-miR-29b
  	hsa-miR-138/mmu-miR-	FL
  	138/rno-miR-138
  	hsa-miR-363/mmu-miR-	FL
  	363/rno-miR-363
  	hsa-miR-142-5p/mmu-	FL
  	miR-142-5p/rno-miR-142-
  	5p
  	hsa-miR-19a/mmu-miR-	FL
  	19a/rno-miR-19a
  	hsa-miR-497/mmu-miR-	FL
  	497/rno-miR-497
  	hsa-miR-144*	FL
  	hsa-miR-16/mmu-miR-	FL
  	16/rno-miR-16
  	hsa-miR-138-1*/mmu-	FL
  	miR-138*/rno-miR-138*
  	hsa-miR-768-5p	FL
  	hsa-miR-30c/mmu-miR-	FL
  	30c/rno-miR-30c
  	hsa-miR-129*	FL
  	hsa-miR-801/mmu-miR-	FL
  	801
  	hsa-miR-34b/mmu-miR-	FL
  	34b-3p
  	hsa-miR-363*/rno-miR-	FL
  	363*
  	hsa-miR-20b*	FL
  	hsa-miR-550	FL
  	hsa-miR-600	FL
  	hsa-miR-196a*/mmu-	FL
  	miR-196a*/rno-miR-196a*
  	hsa-miR-574-3p/mmu-	FL
  	miR-574-3p
  	hsa-miR-620	FL
  	hsa-miR-331-3p/mmu-	FL
  	miR-331-3p/rno-miR-331
  	hsa-let-7e/mmu-let-	FL
  	7e/rno-let-7e
  	hsa-miR-524-5p	FL
  	hsa-miR-197/mmu-miR-	FL
  	197
  	hsa-miR-24-1*/mmu-miR-	FL
  	24-1*/rno-miR-24-1*
  	hsa-miR-519e*	HL
  	hsa-miR-628-3p	HL
  	mghv-miR-M1-7-5p	HL
  	hsa-miR-498	HL
  	hsa-miR-525-5p	HL
  	hsa-miR-520d-5p	HL
  	hsa-miR-551b*	HL
  	hsa-miR-340*/mmu-	HL
  	miR-340-3p/rno-
  	miR-340-3p
  	hsa-miR-889	HL
  	hsa-miR-494/mmu-	HL
  	miR-494/rno-miR-
  	494
  	hsa-miR-874/mmu-	HL
  	miR-874/rno-miR-
  	874
  	hsa-miR-30c-	HL
  	2*/mmu-miR-30c-
  	2*/rno-miR-30c-2*
  	hsa-miR-183*/mmu-	HL
  	miR-183*
  	hsa-miR-25*	HL
  	hsa-miR-513a-5p	HL
  	hsa-miR-198	HL
  	hsa-miR-659	HL
  	mghv-miR-M1-4	HL
  	hsa-miR-129-	HL
  	5p/mmu-miR-129-
  	5p/rno-miR-129
  	ebv-miR-BART13	HL
  	hsa-miR-193b*	HL
  	hsa-miR-422a	HL
  	hsa-miR-503	HL
  	kshv-miR-K12-3	HL
  	hsa-miR-766	HL
  	hsa-miR-516a-5p	HL
  	hsa-miR-125b-	HL
  	1*/mmu-miR-125b-
  	3p/rno-miR-125b-3p
  	hsa-miR-149*	HL
  	ebv-miR-BART6-3p	HL
  	ebv-miR-BART19-3p	HL
  	hsa-miR-671-	HL
  	5p/mmu-miR-671-5p
  	ebv-miR-BART8*	HL
  	hsa-miR-509-3-5p	HL
  	hsa-miR-602	HL
  	ebv-miR-BHRF1-1	HL
  	mghv-miR-M1-7-3p	HL
  	mghv-miR-M1-2	HL
  	hsa-miR-675	HL
  	ebv-miR-BHRF1-2	HL
  	hsa-miR-145*/mmu-	HL
  	miR-145*
  	hsa-miR-296-5p/mmu-	HL
  	miR-296-5p/rno-miR-
  	296*
  	hsa-miR-17*/rno-miR-	HL
  	17-3p
  	hsa-miR-452	HL
  	hsa-miR-943	HL
  	hsa-miR-326/mmu-miR-	HL
  	326/rno-miR-326
  	hsa-miR-652/mmu-miR-	HL
  	652/rno-miR-652
  	hsa-miR-623	HL
  	hsa-miR-194*	HL
  	hsa-miR-557	HL
  	hsa-miR-125a-3p/mmu-	HL
  	miR-125a-3p/rno-miR-
  	125a-3p
  	hsa-miR-425*/mmu-	HL
  	miR-425*
  	hsa-miR-10a*/mmu-	HL
  	miR-10a*/rno-miR-10a-
  	3p
  	hsa-miR-323-3p/mmu-	HL
  	miR-323-3p/rno-miR-
  	323
  	hsa-miR-519e	HL
  	hsa-miR-502-5p	HL
  	hsa-miR-124*/mmu-	HL
  	miR-124*/rno-miR-124*
  	hsa-miR-345	HL
  	hsa-miR-584	HL
  	hsa-miR-654-5p	HL
  	hsa-miR-331-5p/mmu-	HL
  	miR-331-5p
  	hsa-miR-650	HL
  	hsa-miR-202	HL
  	hsa-miR-548b-3p	HL
  	hsa-miR-492	HL
  	hsa-miR-135a*/mmu-	HL
  	miR-135a*
  	ebv-miR-BART20-3p	HL
  	hsa-miR-586	HL
  	hsa-miR-338-5p/mmu-	HL
  	miR-338-5p/rno-miR-
  	338*
  	hsa-miR-92b*	HL
  	hiv1-miR-H1	HL
  	hsa-miR-508-5p	HL
  	hsa-miR-542-5p/mmu-	HL
  	miR-542-5p/rno-miR-
  	542-5p
  	hsa-miR-490-5p	HL
  	hsa-miR-663	HL
  	hsa-miR-433/mmu-miR-	HL
  	433/rno-miR-433

Claims (30)

1. A composition comprising an isolated nucleic acid molecule having at least 80% sequence identity to any one of SEQ ID NOs: 763-1350 or 1565 or a complementary sequence thereof.

2. The composition of claim 1, wherein the isolated nucleic acid molecule is a nucleic acid molecule having at least 90% sequence identity to any one of SEQ ID NOs: 763-1350 or 1565 or a complementary sequence thereof.

3. (canceled)

4. The composition of claim 1, wherein the isolated nucleic acid molecule is a nucleic acid molecule comprising any one of SEQ ID NOs: 763-1350 or 1565 or a complementary sequence thereof.

5. The composition of claim 1, wherein the isolated nucleic acid molecule is a miRNA precursor molecule having the sequence of SEQ ID NOs: 763-1350 or 1565, or a DNA molecule coding the miRNA.

6. The composition of claim 1, wherein the isolated nucleic acid molecule is either single-stranded or at least partially double-stranded.

7. (canceled)

8. The composition of claim 1, wherein the isolated nucleic acid molecule is selected from RNA or DNA molecules.

9. The composition of claim 1, wherein the isolated nucleic acid molecule is a cDNA that corresponds to any one of SEQ ID NOs: 763-1350 or 1565.

10. The composition of claim 1, wherein the isolated nucleic acid molecule comprises at least one modified nucleotide analog.

11. The composition of claim 1, further comprising a carrier or vehicle, wherein the isolated nucleic acid molecule is suitable as a marker or modulator of a B cell malignancy, and wherein the carrier or vehicle is a pharmaceutically acceptable carrier or vehicle suitable for diagnostic or therapeutic applications.

12-14. (canceled)

15. The composition of claim 1, wherein the isolated nucleic acid molecule is operably linked to a recombinant expression vector.

16. A cell comprising the recombinant expression vector of claim 15.

17. (canceled)

18. (canceled)

19. A method of identifying a B cell malignancy in a subject comprising determining the level of expression of at least one microRNA in a sample comprising a B cell taken from the subject relative the level of expression of the at least one microRNA in a control sample, wherein the at least one microRNA is selected from the microRNAs listed in any one of Tables 4 and 7 to 35.

20. (canceled)

21. The method of claim 19, wherein the B cell malignancy is one of at least two potential B cell malignancies, wherein at least two of the potential B cell malignancies are derived from different B cell stages.

22. (canceled)

23. The method of claim 19, wherein the B cell malignancy is selected from chronic lymphocytic leukemia, follicular lymphoma, Hodgkin's lymphoma, activated B-cell diffuse large B cell lymphoma (DLBCL), germinal center-like DLBCL, and Burkitt lymphoma.

24-34. (canceled)

35. A kit comprising one or more sequences selected from a group consisting of

(a) at least one primer sequence that can detect any one of SEQ ID NOs: 763-1350 or 1565, or a combination thereof;

(b) at least one isolated nucleic acid molecule having a sequence of any one of SEQ ID NOs: 1351-1564;

(c) the isolated nucleic acid molecule of claim 1; and

(d) any combination of (a)-(c).

36. (canceled)

37. The kit of claim 35, wherein (a) further comprises enzymes and reagents that are adequate for performing an RT-PCR reaction.

38-41. (canceled)

42. The method of claim 19, further comprising determining whether the B cell malignancy in the sample is Burkitt lymphoma, activated B cell-like diffuse large B cell lymphoma (DLBCL), or germinal center-like DLBCL, comprising determining the level of expression of at least one microRNA in the sample relative to the level of expression of the at least one microRNA in the control sample, wherein the at least one microRNA is selected from the microRNAs listed in Table 7, column “GCBvsBL”; Table 7, column “GCBvsABC”; Table 8, column “ABCvsBL”; Table 10, column “BL miRNA list”; Table 10, column “GCB miRNA list”; Table 11; Table 14, column “BL High”; Table 14, column “ABC High”; Table 14, column “GCB High”; Table 32; or Table 35.

43. The method of claim 42, comprising determining the level of expression of at least one microRNA selected from the microRNAs listed in Table 10, column “BL miRNA list”, and Table 14, column “BL High”; determining the level of expression of at least one microRNA selected from the microRNAs listed in Table 11 and Table 14, column “ABC High”; and determining the level of expression of at least one microRNA selected from the microRNAs listed in Table 10, column “GCB miRNA list”, and Table 14, column “GCB High”.

44. The method of claim 43, wherein at least one microRNA is listed in only one column selected from the columns labeled “GCBvsBL” or “GCBvsABC” in Table 7 or “ABCvsBL” in Table 8.

45. The method of claim 42, wherein at least one microRNA is listed in no more than two columns selected from the columns labeled “GCBvsBL” or “GCBvsABC” in Table 7 or “ABCvsBL” in Table 8.

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