TWI852789B - Blood typing test products, uses of composite fiber nonwoven fabrics, in vitro blood typing test methods and in vitro blood transfusion compatibility test methods - Google Patents

Abstract

The present invention provides a blood typing test product, comprising a composite fiber nonwoven fabric, the composite fiber nonwoven fabric is hydrophilic and has pores; and the surface of the composite fiber nonwoven fabric is divided into a plurality of blocks, each of the blocks is provided with a blood typing antibody, and the blood typing antibodies in different blocks are different from each other. The blood typing test product of the present invention has the advantages of 100% accuracy and low cost. The present invention also provides a use of the composite fiber nonwoven fabric, an in vitro blood typing test method and an in vitro blood transfusion compatibility test method, and has the advantages of simple operation, no need for a test instrument, extremely short time consumption and extremely small amount of blood to complete the test.

Description

Blood typing test products, uses of composite fiber nonwoven fabrics, in vitro blood typing test methods and in vitro blood transfusion compatibility test methods

The present invention relates to a blood typing test product and a blood transfusion compatibility test method, in particular to a blood typing test product using a composite fiber nonwoven fabric. The present invention also relates to the use of the composite fiber nonwoven fabric, and an in vitro blood typing test method and an in vitro blood transfusion compatibility test method using the composite fiber nonwoven fabric.

Everyone needs to check their blood type at some point in their life. For example, if the father's blood type is A, B or AB, and the mother's blood type is O, the fetus' blood type needs to be known first. If the fetus' blood type is different from the mother's, further evaluation is needed for potential hemolytic problems. In addition, everyone needs to know their blood type before donating blood or receiving a blood transfusion due to illness or accident, so as to assess the feasibility of blood transfusion.

Common blood typing methods currently used in medical institutions include the test tube method and the cylindrical gel agglutination method. However, both methods require the use of a centrifuge, especially the cylindrical gel agglutination method, which requires a dedicated centrifuge and is difficult to perform in areas where medical equipment or medical resources are relatively scarce. Therefore, simpler blood typing methods or in vitro blood transfusion compatibility testing methods still need to be developed.

To solve the above problems, the present invention provides a blood type testing product, comprising a composite fiber nonwoven fabric, the composite fiber nonwoven fabric is hydrophilic and has pores; and the surface of the composite fiber nonwoven fabric is divided into a plurality of blocks, each of the blocks is provided with a blood type antibody, and the blood type antibodies in different blocks are different from each other.

According to the present invention, the composite fiber nonwoven fabric has hydrophilicity. In addition, because the composite fiber nonwoven fabric includes a plurality of composite fibers and the composite fibers form pores, the composite fiber nonwoven fabric has pores, so that the composite fiber nonwoven fabric is conducive to adsorbing blood type antibodies and transferring blood, and is conducive to the blood type antibodies distributed in the composite fiber nonwoven fabric and the corresponding blood cells after binding, entangled and fixed to the composite fibers, and red blood cell aggregation is generated in the composite fiber nonwoven fabric, and the blood type is judged by whether the red blood cell aggregation is generated in each block.

In one embodiment, the composite fiber nonwoven fabric has a plurality of composite fibers, and the pores are formed by stacking the fibers to form a fiber mesh structure.

In one embodiment, the thickness of the composite fiber nonwoven fabric is greater than or equal to 0.1 mm. Preferably, the thickness of the composite fiber nonwoven fabric is greater than or equal to 0.2 mm to 0.5 mm, for example, 0.2 mm, 0.24 mm, 0.28 mm, 0.32 mm, 0.36 mm, 0.4 mm, 0.44 mm, 0.48 mm or 0.5 mm. More preferably, the thickness of the composite fiber nonwoven fabric is 0.23 mm to 0.25 mm.

In one embodiment, the weight of the composite fiber nonwoven fabric per square meter is 25 grams to 45 grams, for example: 25 grams, 28 grams, 32 grams, 36 grams, 40 grams, 44 grams or 45 grams. Preferably, the weight of the composite fiber nonwoven fabric per square meter is 29 grams to 31 grams.

In one embodiment, the average diameter of the pores is 2 micrometers (µm) to 5 micrometers.

In one embodiment, the porosity of the composite fiber nonwoven fabric is 30% to 60%, for example, 30%, 35%, 40%, 45%, 50%, 55% or 60%.

In one embodiment, the apparent density of the composite fiber nonwoven fabric is 0.075 g/cm ³ to 0.175 g/cm ³ , for example, 0.075 g/cm ³ , 0.09 g/cm ³ , 0.11 g/cm ³ , 0.13 g/cm ³ , 0.15 g/cm ³ , 0.17 g/cm ³ or 0.175 g/cm ^3. Preferably, the apparent density of the composite fiber nonwoven fabric is 0.12 g/cm ³ to 0.14 g/cm ^3. More preferably, the apparent density of the composite fiber nonwoven fabric is 0.123 g/cm ³ to 0.127 g/cm ³ .

The above apparent density is (weight of the composite fiber nonwoven fabric)/(apparent volume of the composite fiber nonwoven fabric). Preferably, the apparent volume is the external volume of the composite fiber nonwoven fabric, for example, obtained by multiplying the length, width and height of the composite fiber nonwoven fabric.

In one implementation, the blocks are spaced apart from each other.

In one embodiment, the blocks are located on the same surface.

In one embodiment, the content of blood type antibodies contained in any of the blocks is 25 micrograms to 50 micrograms per square centimeter.

In one embodiment, the content of blood type antibodies contained in any of the blocks is 25 micrograms to 50 micrograms per cubic centimeter. Since the blood type antibodies are mainly distributed on the surface of the composite fiber nonwoven fabric, the thickness of the composite fiber nonwoven fabric does not affect the amount of blood type antibodies added in principle.

In one embodiment, the composite fiber nonwoven fabric is hydrophilically modified or treated, so that the composite fiber nonwoven fabric has hydrophilicity.

In one embodiment, the composite fiber nonwoven fabric comprises at least one thermally adhesive composite fiber. Preferably, each thermally adhesive composite fiber has different main components, different doping components or different chemically modified groups.

In one embodiment, the composite fiber nonwoven fabric comprises a first thermally bondable composite fiber and a second thermally bondable composite fiber, and the composite fiber nonwoven fabric is obtained by blending the first thermally bondable composite fiber and the second thermally bondable composite fiber.

In one embodiment, the thermally adhesive composite fiber includes any one of polyethylene fiber, polypropylene fiber and polyester fiber or a combination thereof.

In one embodiment, the thermally adhesive composite fiber is hydrophilically modified or treated, thereby making the thermally adhesive composite fiber hydrophilic.

In one embodiment, the average diameter of the thermally adhesive composite fiber is 2 μm to 5 μm.

In one embodiment, the thermally adhesive composite fiber includes a polypropylene fiber, and the polypropylene fiber is further connected to a hydrophilic agent or has a hydrophilic group.

In one embodiment, the thermally adhesive composite fiber comprises a bicomponent composite fiber of polyethylene and polypropylene, a bicomponent composite fiber of polypropylene and polyester, or a bicomponent composite fiber of polyethylene and polyester.

Preferably, the heat-bondable composite fiber is a two-component composite fiber of polyethylene and polypropylene, and based on the total weight of the heat-bondable composite fiber, the content of the polyethylene or the polypropylene is 10 weight percent to 60 weight percent. More preferably, based on the total weight of the heat-bondable composite fiber, the content of the polyethylene is 40 weight percent to 90 weight percent, and the content of the polypropylene is 10 weight percent to 60 weight percent.

Preferably, the heat-bondable composite fiber is a two-component composite fiber of polypropylene and polyester, and based on the total weight of the heat-bondable composite fiber, the content of the polypropylene or the polyester is 10 weight percent to 60 weight percent. More preferably, based on the total weight of the heat-bondable composite fiber, the content of the polypropylene is 40 weight percent to 90 weight percent, and the content of the polyester is 10 weight percent to 60 weight percent.

Preferably, the heat-bondable composite fiber is a two-component composite fiber of polyethylene and polyester, and based on the total weight of the heat-bondable composite fiber, the content of the polyethylene or the polyester is 10 weight percent to 60 weight percent. More preferably, based on the total weight of the heat-bondable composite fiber, the content of the polyethylene is 40 weight percent to 90 weight percent, and the content of the polyester is 10 weight percent to 60 weight percent.

In one embodiment, the bicomponent composite fiber of polyethylene and polypropylene, the bicomponent composite fiber of polypropylene and polyester, or the bicomponent composite fiber of polyethylene and polyester is either linked to a hydrophilic agent or has a hydrophilic group.

In one embodiment, the thermally adhesive composite fiber has a skin-core structure. Preferably, the skin-core structure is a core structure covered with a skin layer structure, and the melting point of the skin layer structure is lower than the melting point of the core structure.

In one embodiment, the heat-adhesive composite fiber is a two-component side-by-side fiber. Preferably, the two-component side-by-side fiber is an ethylene-propylene side-by-side (ES) composite fiber, which is referred to as ES composite fiber in the market. Currently, ES composite fibers in the market have been improved, so the components are no longer limited to ethylene and propylene, and the components contained in the ES composite fibers can be the same or different. For example, ES Fibervisions sells composite fibers with both the sheath and core made of 100% polypropylene (e.g., the sheath is made of copolypropylene with a melting point of 130°C, and the core is made of polypropylene with a melting point of 161°C); or composite fibers with the core made of polyester and the sheath made of polyethylene or polypropylene.

In one embodiment, the thermally adhesive composite fiber has a sheath-core structure, and the thermally adhesive composite fiber includes a bicomponent composite fiber with a sheath structure of polyethylene and a core structure of polypropylene, a bicomponent composite fiber with a sheath structure of polypropylene and a core structure of polyester, or a bicomponent composite fiber with a sheath structure of polyethylene and a core structure of polyester.

Preferably, the thermally adhesive composite fiber is a bicomponent composite fiber having a sheath structure of polyethylene and a core structure of polypropylene, and the content of polyethylene and polypropylene is the same as that of the above-mentioned bicomponent composite fiber of polyethylene and polypropylene.

Preferably, the thermally adhesive composite fiber is a bicomponent composite fiber having a sheath structure of polypropylene and a core structure of polyester, and the contents of polypropylene and polyester are the same as those of the above-mentioned bicomponent composite fiber of polypropylene and polyester.

Preferably, the thermally adhesive composite fiber is a bicomponent composite fiber having a sheath structure of polyethylene and a core structure of polyester, and the content of polyethylene and polyester is the same as that of the above-mentioned bicomponent composite fiber of polyethylene and polyester.

In one embodiment, the composite fiber nonwoven fabric uses the same layer as the inner layer of the medical mask. Medical masks generally include three layers: (i) the outer layer of the mask: generally a water-repellent spunbond nonwoven fabric, a common material is polypropylene; (ii) the filter layer of the mask: generally a high-density melt-blown nonwoven fabric, a common material is polypropylene; and (iii) the inner layer of the mask: generally a polypropylene spunbond nonwoven fabric connected to a hydrophilic agent, a bicomponent composite fiber of polyethylene and polypropylene, or a bicomponent composite fiber of polyethylene and polyester, and in the bicomponent composite fiber portion, based on the total weight of the bicomponent composite fiber, the content of any component is 10 weight percent to 60 weight percent. According to the present invention, the blood typing test can be easily completed by using the inner layer of the medical mask, and the accuracy is the same as that of the test tube method and the cylindrical gel agglutination method, which is 100%.

In one embodiment, the blood typing product further comprises physiological saline for removing blood.

According to the present invention, after the composite fiber nonwoven fabric absorbs blood and waits for 1 to 4 minutes, the composite fiber nonwoven fabric that absorbs blood is further washed with physiological saline water, or the composite fiber nonwoven fabric that absorbs blood is immersed in the physiological saline water. If the blood type antibodies in the block combine with the red blood cells with the corresponding antigen to form red blood cell aggregates, the physiological saline water will not be able to remove the red blood cell aggregates in the block, and the block will remain bloody; the blood color in the remaining blocks will be removed, and the blood type can be determined based on whether the red blood cell aggregates are formed in each block.

Preferably, during the waiting process, the blood-absorbing composite fiber nonwoven fabric is in a static state.

Preferably, the concentration of the physiological saline is 8.5 grams of sodium chloride per liter of pure water. Although physiological saline is easily available, the blood typing test product of the present invention provides physiological saline in one embodiment, which will be more convenient for the general public to operate by themselves.

In one embodiment, the distribution pattern of the blood type antibodies in each block on the composite fiber nonwoven fabric corresponds to the English text of the blood type. For example, when type A antibodies react with type A blood, the composite fiber nonwoven fabric will only have the color of type A blood because the distribution pattern of type A antibodies is in the shape of letter A. The blood type can be directly determined without the need for other markers or reading instructions to know which blood type each block corresponds to.

In one embodiment, the blood type test product further comprises a substrate for carrying the composite fiber nonwoven fabric. Preferably, the substrate is in a long strip shape for easy hand-holding and placement of the composite fiber nonwoven fabric that absorbs blood into a container containing physiological saline, such as a test tube containing physiological saline.

Preferably, the composite fiber nonwoven fabric is fixed to the substrate, for example, the composite fiber nonwoven fabric is adhered to the substrate.

The present invention further provides a use of the composite fiber nonwoven fabric for in vitro blood type testing, wherein the composite fiber nonwoven fabric is hydrophilic and has pores; and the surface of the composite fiber nonwoven fabric is divided into a plurality of blocks, each of which is provided with a blood type antibody, and the blood type antibodies in different blocks are different from each other.

The present invention also provides an in vitro blood type test method, comprising: A composite fiber nonwoven fabric is provided, the composite fiber nonwoven fabric is hydrophilic and has pores; and the surface of the composite fiber nonwoven fabric is divided into a plurality of blocks, each of the blocks is provided with a blood type antibody, and the blood type antibodies in different blocks are different from each other; A blood to be tested is provided to each of the blocks, and after waiting for 1 to 4 minutes, a composite fiber nonwoven fabric adsorbing the blood is obtained; and Immerse the blood-absorbed composite fiber nonwoven fabric in physiological saline. If the blood-absorbed composite fiber nonwoven fabric retains blood color, it means that the blood type of the blood to be tested corresponds to the blood type antibody. If the blood-absorbed composite fiber nonwoven fabric does not retain blood color, it means that the blood type of the blood to be tested is O type.

The in vitro blood typing test method of the present invention does not require the use of a centrifuge and takes very little time, so it has the advantages of low cost, simple operation and no need for testing equipment.

Preferably, the volume of the blood to be tested in each block is greater than 0 ml to less than or equal to 0.5 ml, for example: 0.01 ml, 0.02 ml, 0.03 ml, 0.04 ml, 0.05 ml, 0.06 ml, 0.07 ml, 0.1 ml, 0.3 ml or 0.5 ml. More preferably, the volume of the blood to be tested in each block is 0.02 ml to 0.03 ml.

Preferably, the waiting time is 1 to 2 minutes.

The present invention also provides an in vitro blood transfusion compatibility test method, comprising: Preparing a composite fiber nonwoven fabric, and the composite fiber nonwoven fabric is hydrophilic and has pores; Providing the blood serum of the blood recipient on the composite fiber nonwoven fabric, and waiting for 1 to 4 minutes, and then providing the blood of the potential blood donor to the place where the blood serum of the blood recipient is adsorbed, and waiting for 1 to 4 minutes, so as to obtain a composite fiber nonwoven fabric adsorbing blood; and Immerse the blood-absorbing composite fiber nonwoven fabric in physiological saline. If the blood-absorbing composite fiber nonwoven fabric retains blood color, it means that the potential blood donor cannot donate blood to the blood recipient; if the blood-absorbing composite fiber nonwoven fabric does not retain blood color, it means that the potential blood donor can donate blood to the blood recipient.

Although O-type blood does not have A-type and B-type antigens on its red blood cells and can in principle be transfused to patients with different blood types, the plasma of O-type blood still contains anti-A antibodies and anti-B antibodies, which may pose risks to recipients of different blood types. Therefore, in medical practice, cross-testing is still performed before blood transfusion to reduce the health risks of blood recipients.

Preferably, the composite fiber nonwoven fabric comprises an inner layer of a medical mask. Since both medical masks and physiological saline are easily available, the present invention can easily perform blood transfusion compatibility testing to reduce the health risks of blood recipients. Therefore, the present invention can particularly benefit blood recipients in areas where medical equipment or medical resources are relatively scarce.

Preferably, the serum of the recipient and the blood of the potential donor are each greater than or equal to 0.02 ml. Preferably, each is 0.02 ml to 0.1 ml, for example, 0.02 ml, 0.04 ml, 0.05 ml, 0.07 ml, 0.09 ml or 0.1 ml.

The present invention further provides a composite fiber nonwoven fabric for use in in vitro blood transfusion compatibility testing, wherein the composite fiber nonwoven fabric is hydrophilic and has pores.

In summary, the blood typing test product, the use of the composite fiber nonwoven fabric for in vitro blood typing test, the in vitro blood typing test method, the in vitro blood transfusion compatibility test method and the use of the composite fiber nonwoven fabric for in vitro blood transfusion compatibility test of the present invention all have the advantages of 100% accuracy, low cost, simple operation, no need for testing equipment, extremely short time consumption and extremely small amount of blood to complete the test.

Several operation methods are provided below to illustrate the implementation methods of the present invention; those skilled in the art can easily understand the advantages and effects that can be achieved by the present invention through the contents of this manual, and make various modifications and changes without departing from the spirit of the present invention to implement or apply the contents of the present invention.

Example 1: Blood typing product

As shown in FIG1 , the blood type test product of the present invention comprises a composite fiber nonwoven fabric 1, the surface of which is divided into a plurality of blocks 10A and 10B, each of which is provided with blood type antibodies, and the blood type antibodies in different blocks 10A and 10B are different from each other. Specifically, in medical practice, in order to distinguish between type A antibodies and type B antibodies, commercially available type A antibodies will be additionally added with blue dye, and type B antibodies will be additionally added with yellow dye to avoid confusion, so block 10A is blue, indicating that the commercially available type A antibodies have been dripped and adsorbed to block 10A, and block 10B is yellow, indicating that the commercially available type B antibodies have been dripped and adsorbed to block 10B. In addition, the long strip substrate 2 supports the composite fiber non-woven fabric 1 to facilitate operation.

As shown in FIG2 , the composite fiber nonwoven fabric 1 is hydrophilic. In addition, the composite fiber nonwoven fabric 1 includes a plurality of composite fibers 30, and the composite fibers 30 form pores 40, so that the composite fiber nonwoven fabric 1 has pores 40; wherein the blood type antibody 50 is connected to the composite fibers 30, for example: the blood type antibody 50 is in direct contact with the composite fibers 30, the blood type antibody 50 is attached to the composite fibers 30, or the blood type antibody 50 is adhered to the composite fibers 30. As shown in FIG3 , when the blood type antibody 50 encounters the red blood cell 3 with the same blood type antigen, the red blood cell aggregate 4 will be generated and fixed on the composite fibers 30 or in the pores 40 to form the red blood cell aggregate 4, so that the blood type can be determined according to whether the red blood cell aggregate 4 is generated in each block. It can be seen that the composite fiber nonwoven fabric 1 of the present invention can be used for blood type testing.

Example 2: Blood typing method

As shown in FIG. 1 and FIG. 2 , the in vitro blood type detection method of the present invention comprises: a composite fiber nonwoven fabric 1 is provided, and the composite fiber nonwoven fabric 1 is hydrophilic. In addition, the composite fiber nonwoven fabric 1 comprises a plurality of composite fibers 30, and the composite fibers 30 form pores 40, so that the composite fiber nonwoven fabric 1 has pores 40; and the surface of the composite fiber nonwoven fabric 1 is divided into a plurality of blocks 10A and 10B, and the blocks 10A and 10B are respectively provided with blood type antibodies 50, and the blood type antibodies 50 in different blocks 10A and 10B are different from each other. Specifically, the composite fiber non-woven fabric 1 is taken from the inner layer of a commercially available medical mask (brand: Meide; model: surgical protective mask) (i.e., a composite fiber non-woven fabric using a two-component composite fiber of polyethylene and polypropylene), with a thickness of 0.24 mm, and is bonded to a plastic substrate 2; wherein the substrate 2 has a length of 150 mm, a width of 5 mm, and a thickness of 0.05 mm. One drop of a blue commercially available type A antibody solution (25 μl in total) is added to block 10A, and one drop of a yellow commercially available type B antibody solution (25 μl in total) is added to block 10B, thereby obtaining a blood typing product as shown in FIG. 1 ; wherein the antibody content in block 10A and block 10B is each approximately 35 μg per square centimeter.

As shown in FIG4 , a blood to be tested is provided to each of the blocks 10A and 10B, and after waiting for 1 to 4 minutes, a composite fiber nonwoven fabric 1 absorbing blood is obtained. Specifically, 1 drop (25 μl) of the blood to be tested is added to the blocks 10A and 10B respectively, and after waiting for 3 minutes, the appearance of the composite fiber nonwoven fabric 1 absorbing blood is shown in FIG4 .

As shown in Fig. 5 and Fig. 6, the composite fiber nonwoven fabric 1 absorbing blood is immersed in physiological saline. If the composite fiber nonwoven fabric absorbing blood has blood color, it means that the blood type of the blood to be tested corresponds to the blood type antibody. If the composite fiber nonwoven fabric absorbing blood has no blood color, it means that the blood type of the blood to be tested is type O. Specifically, 1 ml of physiological saline is put into a test tube, and as shown in Fig. 5, the composite fiber nonwoven fabric absorbing blood is immersed in the physiological saline and shaken up and down for 10 to 15 seconds. It can be found that the blood in block 10A slides off. Finally, as shown in Fig. 6, only block 10B has blood color. Since block 10B adsorbs commercially available type B antibodies and leaves blood color, and block 10A adsorbs commercially available type A antibodies but leaves no blood color, it can be known that the blood type of the blood to be tested is type B.

Implementation 1: Accuracy test

(I) Experimental procedures: This experiment was divided into three groups: the in vitro blood typing method of the present invention, the test tube method and the cylindrical gel agglutination method, and 205 samples were tested for blood typing; among them, 46 were type A samples, 42 were type B samples, 106 were type O samples, and 11 were type AB samples. The operating procedures of each group are as follows:

1. The in vitro blood typing method of the present invention:

The steps are the same as those in Example 2. In addition, by naked eye observation, if the block adsorbing type A antibodies has blood color, and the block adsorbing type B antibodies has no blood color, it indicates that the blood type of the blood sample is type A; if the block adsorbing type A antibodies has no blood color, and the block adsorbing type B antibodies has blood color, it indicates that the blood type of the blood sample is type B; if the block adsorbing type A antibodies has blood color, and the block adsorbing type B antibodies also has blood color, it indicates that the blood type of the blood sample is type AB; and if the block adsorbing type A antibodies has no blood color, and the block adsorbing type B antibodies also has no blood color, it indicates that the blood type of the blood sample is type O.

2. Test tube method:

Prepare 2 test tubes: A and B. Add 1 ml of physiological saline to each tube. Add 2 drops of commercially available A-type antibody solution to A and 2 drops of commercially available B-type antibody solution to B. Add 1 drop (i.e. 25 μl) of blood sample (i.e. 3% red blood cell suspension) to A and B respectively and mix them. Centrifuge at 3400 rpm for 15 seconds and observe with the naked eye whether there is agglutination reaction in the test tube: the appearance of a precipitate indicates agglutination reaction. If the blood cells scatter with the shaking of the test tube, it means there is no agglutination reaction.

In addition, if a precipitate appears in test tube A and no precipitate appears in test tube B, it means that the blood type of the blood sample is type A; if no precipitate appears in test tube A and a precipitate appears in test tube B, it means that the blood type of the blood sample is type B; if a precipitate appears in test tube A and a precipitate also appears in test tube B, it means that the blood type of the blood sample is type AB; and if no precipitate appears in test tube A and no precipitate appears in test tube B, it means that the blood type of the blood sample is type O.

3. Cylindrical gel agglutination method:

According to the operating instructions of the blood bank's dedicated centrifuge (ORTHO BioVue ^® ), the gel containing type A antibodies and the gel containing type B antibodies were made into gel cards, wherein the gel cards included microtube A (containing type A antibodies) and microtube B (containing type B antibodies). One drop (i.e. 25 μl) of blood sample (i.e. 3% red blood cell suspension) was dropped into microtube A and microtube B, and then centrifuged at 100g for 10 minutes. The microtubes were then observed with the naked eye to see if there was an agglutination reaction: if the red blood cells remained in the upper layer of the microtube, it indicated an agglutination reaction; if the red blood cells settled to the bottom of the microtube, it indicated no agglutination reaction.

In addition, if the red blood cells remain in the upper layer of microtube A and the red blood cells settle to the bottom of microtube B, it means that the blood type of the blood sample is type A; if the red blood cells settle to the bottom of microtube A and the red blood cells remain in the upper layer of microtube B, it means that the blood type of the blood sample is type B; if the red blood cells remain in the upper layer of microtubes A and B, it means that the blood type of the blood sample is type AB; and if the red blood cells settle to the bottom of microtubes A and B, it means that the blood type of the blood sample is type O.

(II) Experimental results:

The results obtained by each group for 205 samples were consistent, indicating that the in vitro blood typing method of the present invention is as accurate as the test tube method and cylindrical gel agglutination method commonly used in current hospitals, and has a 100% accuracy rate. In addition, the in vitro blood typing method of the present invention does not require a centrifugation step, so the cost of purchasing a centrifuge and the time of centrifugation can be saved, so the present invention has the advantages of low cost and simple operation.

In summary, the blood typing test product, the use of the composite fiber nonwoven fabric for in vitro blood typing test, the in vitro blood typing test method, the in vitro blood transfusion compatibility test method and the use of the composite fiber nonwoven fabric for in vitro blood transfusion compatibility test of the present invention all have the advantages of 100% accuracy, low cost, simple operation, no need for testing equipment, extremely short time consumption and extremely small amount of blood to complete the test.

1: Composite fiber nonwoven fabric

2: Substrate

3: Red blood cells

4: Red blood cell aggregation

10A,10B: Block

30: Composite fiber

40: Porosity

50: Blood type antibodies

Figure 1 is an appearance photograph of the blood typing test product of the present invention. Figure 2 is an enlarged schematic diagram of the blood typing test product of the present invention. Figure 3 is an enlarged schematic diagram of the blood typing test product of the present invention forming red blood cell agglomeration. Figure 4 is an appearance photograph of the blood typing test product of the present invention adsorbing blood. Figure 5 is an appearance photograph of the blood typing test product of the present invention after adsorbing blood and then immersed in physiological saline, resulting in blood sliding off the area where no red blood cell agglomeration is produced. Figure 6 is an appearance photograph of the blood typing test product of the present invention presenting the test results.

without

1: Composite fiber nonwoven fabric

10A,10B: Block

2: Substrate

Claims (12)

A blood type testing product comprises a composite fiber nonwoven fabric, which is hydrophilic and has pores; and the surface of the composite fiber nonwoven fabric is divided into a plurality of blocks, each of which is provided with a blood type antibody, and the blood type antibodies in different blocks are different from each other. The blood typing test product as described in claim 1, wherein the composite fiber nonwoven fabric comprises at least one thermally bondable composite fiber, and the thermally bondable composite fiber comprises any one of polyethylene fiber, polypropylene fiber and polyester fiber or a combination thereof. The blood typing test product as described in claim 2, wherein the thermally adhesive composite fiber comprises a polypropylene fiber, and the polypropylene fiber is further connected to a hydrophilic agent or has a hydrophilic group; or the thermally adhesive composite fiber comprises a bicomponent composite fiber of polyethylene and polypropylene, a bicomponent composite fiber of polypropylene and polyester, or a bicomponent composite fiber of polyethylene and polyester. The blood type testing product as described in claim 1, wherein the distribution pattern of the blood type antibodies in each block arranged on the composite fiber nonwoven fabric corresponds to the English text of its blood type. The blood type testing product as described in any one of claim items 1 to 4 further comprises physiological saline. The blood typing test product as described in any one of claims 1 to 4 further comprises a substrate. A composite fiber nonwoven fabric is used for in vitro blood type testing, wherein the composite fiber nonwoven fabric is hydrophilic and has pores; and the surface of the composite fiber nonwoven fabric is divided into a plurality of blocks, each of which is provided with a blood type antibody, and the blood type antibodies in different blocks are different from each other. An in vitro blood type test method includes: providing a composite fiber nonwoven fabric, the composite fiber nonwoven fabric is hydrophilic and has pores; and the surface of the composite fiber nonwoven fabric is divided into a plurality of blocks, each of the blocks is provided with a blood type antibody, and the blood type antibodies in different blocks are different from each other; providing a blood to be tested to each block, and waiting for 1 minute to 4 minutes Afterwards, a composite fiber nonwoven fabric that absorbs blood is obtained; and the composite fiber nonwoven fabric that absorbs blood is immersed in physiological saline. If the composite fiber nonwoven fabric that absorbs blood retains blood color, it means that the blood type of the blood to be tested corresponds to the blood type antibody. If the composite fiber nonwoven fabric that absorbs blood does not retain blood color, it means that the blood type of the blood to be tested is O type. The in vitro blood typing method as described in claim 8, wherein the volume of the blood to be tested in each block is greater than 0 ml and less than or equal to 0.5 ml. An in vitro blood transfusion compatibility test method includes: preparing a composite fiber nonwoven fabric, which is hydrophilic and porous; providing the blood serum of a blood recipient on the composite fiber nonwoven fabric, and waiting for 1 to 4 minutes, and then providing the blood of a potential blood donor to the place where the blood serum of the blood recipient is adsorbed, and waiting for 1 to 4 minutes to obtain a composite fiber nonwoven fabric adsorbing blood; and immersing the composite fiber nonwoven fabric adsorbing blood in physiological saline. If the composite fiber nonwoven fabric adsorbing blood retains blood color, it means that the potential blood donor cannot donate blood to the blood recipient; if the composite fiber nonwoven fabric adsorbing blood does not retain blood color, it means that the potential blood donor can transfuse blood to the blood recipient. The in vitro blood transfusion compatibility test method as described in claim 10, wherein the composite fiber nonwoven fabric comprises an inner layer of a medical mask, and the serum of the blood recipient and the blood of the potential blood donor are each greater than or equal to 0.02 ml. A composite fiber nonwoven fabric is used for in vitro blood transfusion compatibility testing, wherein the composite fiber nonwoven fabric is hydrophilic and has pores.