TWI653060B - Biological fiber membrane and fabrication method thereof - Google Patents

Abstract

A biofiber membrane formed by a bacterium of the genus Gluconobacter, and the biofiber membrane has: a first skin layer and a second skin layer; and a three-dimensional network structure bonded between the first skin layer and the second skin layer , thereby enhancing the moisturizing properties of the dressing and carrying more active ingredients. The invention further provides a method for preparing a biofiber membrane.

Description

Biofiber membrane and its preparation method

The present invention relates to a biofiber membrane, and more particularly to a biofiber membrane that is applied to the skin.

At present, in the medical field, most of the dressings for wound care use cotton pads or gauze, but such dressing products have poor antibacterial properties, high probability of wound infection, easy to produce sticky wounds, and are difficult to remove when changing dressings. The shortcomings.

Subsequent to the replacement of gauze and cotton pad with a non-woven dressing, the dressing of the non-woven type has better liquid absorbing ability and provides a moist environment to help the wound repair. However, when the liquid or water absorbed by the non-woven fabric is gradually lost, After that, it is also prone to the problem of wound sticking.

On the other hand, in addition to the basic needs of life, modern people pay more attention to beauty care, especially the maintenance of the face is the focus of the beauty industry, so the development of a variety of mask products. There are many types of masks of the prior art, such as a mud mask, a tear-off mask, and a sheet-like mask.

Although the mud mask contains maintenance ingredients or minerals, it needs to be rinsed after applying the mask. The maintenance ingredients are difficult to be absorbed by the skin. Because of the high mineral content, more preservatives must be added to prevent the bacteria from being wet. Growing in mud. Peeling and peeling surface The main components of the membrane are polymer glue, water and alcohol, and promote the blood circulation of the skin by raising the temperature of the epidermis. However, since the tear-off mask needs to wait until the mask is dry, it can be removed, and the tearing process may cause damage to sensitive skin. In addition, the tear-off mask is not suitable for dry skin, and is not suitable for dry skin. As for the sheet-like mask, it is mainly a single-layer sheet which is adsorbed with an effective essence, and can be used for the maintenance of various skin types by adjusting the ingredients, and although it does not need to be rinsed after application, it has no cleaning effect. The above-mentioned sheet-like mask is often made of a single-layer nonwoven fabric. When the user uses the non-woven fabric that absorbs the essence, the high concentration of the essence is often used due to the rapid evaporation of moisture in the non-woven fabric, which not only causes waste, but also fails to solve the problem of water evaporation.

Therefore, there is still a need to develop a novel dressing product.

In view of the above-mentioned shortcomings of the prior art, the present invention provides a biofibrous membrane formed by a bacterium belonging to the genus Gluconobacter, and having the first surface layer and the second surface layer and bonded to the first surface layer And a three-dimensional network structure between the second surface layer, and the density of the three-dimensional network structure is smaller than the density of the first surface layer and the second surface layer.

In one embodiment, the three-dimensional network structure is comprised of the plurality of biofibers.

Furthermore, the three-dimensional network structure has a plurality of backbone fibers parallel to each other and interlaminar fibers interlaced with any two of the backbone fibers. The backbone fiber and the interlayer fiber are all biofibers, and the diameter of the backbone fiber is greater than or equal to the diameter of the interlayer fiber.

In yet another embodiment, the biofiber membrane comprises an active ingredient or a drug. The active ingredient can be a moisturizer, a whitening ingredient, an anti-wrinkle ingredient, and an exfoliating substance. Ingredients, growth factors or enzymes.

The invention provides a method for preparing a bio-fiber membrane, comprising: providing a container for accommodating a culture solution, wherein the culture solution has a carbon source and peptone in a weight ratio of 5:1:1 to 4:1:1. And a yeast extract; and cultivating the bacterium of the genus Gluconobacter in the culture solution for 24 to 96 hours.

The biofibrous membrane of the invention is formed by the genus Gluconobacter, and the first surface layer and the second surface layer of the biofiber membrane have a three-dimensional network structure, thereby enhancing the moisturizing property of the dressing and carrying Multiple active ingredients.

1‧‧‧Biofibrous membrane

10a‧‧‧ first surface

10b‧‧‧ second surface

101‧‧‧Three-dimensional network structure

101a‧‧‧ backbone fiber

101b‧‧‧Interlayer fiber

12‧‧‧ fiber

Figure 1 is a schematic view showing the structure of the biofiber membrane of the present invention; 2A is a scanning electron microscope (SEM) photograph showing a three-dimensional network structure of the biofiber membrane of the present invention, and FIG. 2B is a side view showing a biofiber membrane of the present invention; 3A and 3B are respectively showing a 500-times enlarged SEM photograph of the biofiber membrane of the present invention and a 500-times enlarged SEM photograph of a conventional biofiber membrane; Fig. 4 is a graph showing the results of the permeability test of the biofiber membrane of the present invention and a commercially available conventional biofiber membrane, wherein Fig. 4(a) shows the test results of the biofiber membrane of the present invention, and Fig. 4(b) is commercially available. Test results of traditional biofiber membranes.

The other embodiments of the present invention will be readily understood by those skilled in the art from this disclosure.

It should be noted that the structures, proportions, sizes, etc. shown in the drawings of the present specification are only used to cope with the contents disclosed in the specification for the understanding of those skilled in the art. And reading is not intended to limit the conditions for the implementation of the present invention, and therefore does not have technical significance, any modification of the structure, change of the proportional relationship or adjustment of the size, without affecting the effects and functions of the present invention. For the purpose of achieving the same, it should still fall within the scope of the technical content disclosed by the present invention. In the meantime, the terms "first", "second", "upper" and "one" are used in the description, and are not intended to limit the scope of the invention. Changes or adjustments in the relative relationship are considered to be within the scope of the present invention.

The term "parallel" as used herein refers to a form in which a plurality of backbone fibers extend in the same direction, for example, the length or width direction of a biofiber membrane.

The present invention provides a biofiber membrane formed by a bacterium belonging to the genus Gluconobacter, and the biofiber membrane has a first surface layer and a second surface layer and a three-dimensional network structure bonded to the first surface layer and the second surface layer. Between the skin layers, and the density of the three-dimensional network structure is less than the density of the first skin layer and the second skin layer.

The biofiber membrane of the present invention refers to a person obtained by culturing a microorganism. The present invention finds that the biobacteria film formed by the bacterium of the genus Acetobacter in the mannitol, peptone, yeast extract and agar has a complex three-dimensional three-dimensionally wound biofiber .

In a specific embodiment for producing a biofiber membrane, the fermentation culture of the strain is started. First, a culture solution is provided, and the culture solution is placed in a container, and the composition of the culture solution includes a material selected from the group consisting of animal glue, gum arabic, and ocean. Vegetable gum, and other conventional components, but the culture solution also needs to have a carbon source, such as mannitol, glucose or a combination thereof, other components such as peptone and yeast extract, and the weight of the carbon source, peptone and yeast extract The ratio can be between 5:1:1 and 4:1:1. Then controlling the pH value of the culture solution to be acidic, For example, the pH is between 0.5 and 6, and the initial microbial concentration range can be controlled at 102 to 105 cells/ml, and the cells are allowed to stand at 25 to 28 ° C for 24 to 96 hours, since a flat container can be used. The three-dimensional network structure is in the form of a flat film, and then, after being taken out for 24 hours to 72 hours, the biofiber membrane of the present invention is obtained.

The biofiber membrane is tested to have a thickness of at least 20 μm, such as 20 to 30 μm, or such as 20 to 26 μm or 24 to 26 μm, while the biofiber membrane of the present invention has a diameter of about 20 to 100 nm. Further, the biofiber membrane has a biofiber amount per unit area of 0.005 to 0.008 g/cm ² .

As shown in Fig. 1, the biofiber membrane 1 of the present invention includes a first skin layer 10a and a second skin layer 10b, and a three-dimensional network structure 101, which is bonded between the first skin layer 10a and the second skin layer 10b.

In addition, referring to Fig. 2A, it is shown that the second skin layer 10b is extended with a three-dimensional network structure 101 which is bonded to the fibers 12 of a film.

As shown, the three-dimensional network structure 101 extends from the second skin layer 10b to the fibers 12 of the film, and the three-dimensional network structure 101 is composed of a plurality of biofibers. Specifically, the three-dimensional network structure 101 has a plurality of backbone fibers 101a parallel to each other and an interlayer fiber 101b interlaced with any two of the backbone fibers 101a. Thus, the adjacent two of the backbones are connected in the horizontal direction and the vertical direction. The fiber 101a is formed to form a three-dimensional mesh structure 101. The backbone fiber 101a and the interlayer fiber 101b are both the biofiber, and as shown, the diameter of the backbone fiber 101a is greater than or equal to the diameter of the interlayer fiber 101b.

Referring to FIG. 2B, a side view of the bio-fiber membrane 1 is shown, wherein the bio-fiber membrane 1 also has a plurality of backbone fibers 101a that are parallel to each other or extend in the longitudinal direction or the width direction of the bio-fiber membrane 1, and The backbone fiber 101a is interwoven The interlayer fiber 101b, in this embodiment, has a thickness of 20 to 30 μm. Furthermore, as shown, the density of the three-dimensional network structure is less than the density of the two skin layers of the bio-fiber membrane 1, and the active ingredient or drug can be adsorbed between the two skin layers to provide a moisturizing effect.

Further, the biofiber membrane can be prepared by temporarily or permanently bonding the membrane body using a substrate, but in any case, the preparation method can omit the aforementioned step of inverting the flat three-dimensional network structure in the container. For example, in the embodiment of Fig. 2A, a cloth film is used as a substrate.

Referring to Figures 3A and 3B, respectively, a 500-times enlarged SEM photograph of the biofiber membrane of the present invention and a 500-times enlarged SEM photograph of a conventional biofiber membrane are shown.

As shown in Fig. 3A, the surface of the bio-fiber membrane of the present invention is flat, and the strips shown in the figure are the fibers of the film substrate under the film body, so that the surface of the bio-fiber membrane of the present invention is very flat. However, the surface of the conventional biofiber membrane shown in Fig. 3B has many wrinkles. Therefore, it is a kind of poor adhesion of the bio-fiber membrane to the skin, which affects the touch and causes poor absorption of the drug or active ingredient.

Biomass and water content per unit area of biofiber membrane of the present invention

The bio-fiber membrane of the present invention was taken and cut into 16 samples of a size of 5 cm x 5 cm, and then dried at 60 ° C for 10 minutes, and the dried sample was weighed and dried. The dry weight of each sample was divided by the area of the sample, and the amount of biofiber per unit area of the biofiber membrane was measured to be 0.005 to 0.008 g/cm ² .

The samples were immersed in water for 5 minutes to weigh the wet weight of the samples. When measuring the water content per unit area, the calculation was based on the following formula: water content per unit area = (wet weight - dry species) / Sample area

The bio-fiber membrane of the present invention has been tested to have a water content per unit area of from 0.06 to 0.14 g/cm ² .

In contrast, the amount of biofiber per unit area of a commercially available conventional biofiber membrane is usually only 0.001 g/cm ² and the water content is only 0.04 g/cm ² . Therefore, the bio-fiber membrane of the present invention can carry more water or active ingredients, and has a more moisturizing effect.

Permeability test of biofiber membrane of the invention

The bio-fiber membrane of the present invention and the commercially available conventional bio-fiber membrane are respectively placed on a substrate, and then the same amount of the dyed essence is added to the bio-fiber membrane of the present invention and the commercially available conventional bio-fiber membrane, and wait for 10 seconds. The clock was visually observed for the penetration of the substrate.

As shown in Fig. 4, Fig. 4(a) is a test result of the biofiber membrane of the present invention, and Fig. 4(b) is a test result of a commercially available conventional biofiber membrane, wherein a commercially available conventional biofiber membrane is just When the essence is added dropwise, the essence does not spread well. After waiting for 10 seconds, it is naturally unable to effectively penetrate the essence into the substrate, but the biofiber membrane of the present invention has better emphasity diffusion and significant penetration. Sex.

In summary, the bio-fiber membrane of the present invention is applied to the skin, especially for use as a mask, so the bio-fiber membrane may comprise an active ingredient or a drug. Before the fermentation culture of the strain is completed, the active ingredient or the drug is put into the three-dimensional network structure, and the preparation of the biofiber membrane is completed, and the active ingredient or the drug is contained in the membrane body, and an example of the active ingredient is These include moisturizers, whitening ingredients, anti-wrinkle ingredients, exfoliating ingredients, growth factors or enzymes. The drug may be a massage drug, which may be a paste or a liquid, such as a massage or a squeezing essential oil. Since the biofiber membrane of the present invention has a three-dimensional network structure, the active ingredient or the drug is not easily exposed, but the present invention is After the bio-fiber membrane is attached to the body surface or the skin, it is applied to the bio-fiber membrane through artificial massage or other contact devices to facilitate the release of the active ingredient or the drug. Further, it can also be assisted by heating. Active The release of the drug or drug.

The above embodiments are intended to illustrate the principles of the invention and its effects, and are not intended to limit the invention. Any of the above-described embodiments may be modified by those skilled in the art without departing from the spirit and scope of the invention. Therefore, the scope of protection of the present invention should be as set forth in the appended claims.

Claims (10)

a biofibrous membrane formed by a bacterium of the genus Gluconobacter, and the biofibrous membrane has: a first surface layer and a second surface layer; and a three-dimensional network structure bonded to the first surface layer and the second surface layer And the density of the three-dimensional network structure is smaller than the density of the first skin layer and the second skin layer, wherein the three-dimensional network structure is composed of a plurality of biofibers having a diameter of 20 to 100 nm, and has a plurality of backbones parallel to each other And a plurality of interlaminar fibers interlaced with adjacent two of the backbone fibers, wherein the backbone fibers and the interlaminar fibers are bio-fibers, and the diameter of the backbone fibers is greater than or equal to the diameter of the inter-layer fibers. The bio-fiber membrane according to claim 1, wherein the backbone fiber extends in a longitudinal direction or a width direction of the bio-fiber membrane. The bio-fiber membrane of claim 1, wherein the active ingredient or drug is included. The bio-fiber membrane of claim 3, wherein the active ingredient is a moisturizer, a whitening component, an anti-wrinkle component, an exfoliating component, a growth factor or an enzymes. The bio-fiber membrane according to claim 1, wherein the bacterium belonging to the genus Gluconobacter is formed in a culture solution having mannitol, peptone, yeast extract and agar. The bio-fiber membrane according to claim 1, wherein the bio-fiber membrane has a biofiber amount per unit area of 0.005 to 0.008 g/cm ² . The biofiber membrane of claim 1, which has a thickness of at least 20 μm. The biofiber membrane of claim 7, which has a thickness of 20 to 30 μm. A method for preparing a biofiber membrane, comprising: providing a container containing a culture solution, wherein the culture solution has a carbon source, peptone and yeast extract in a weight ratio of 5:1:1 to 4:1:1 And culturing the bacterium of the genus Gluconobacter in the culture solution for 24 to 96 hours to obtain a biofiber membrane as described in claim 1. The method of claim 9, wherein the culture solution has a pH of 0.5 to 6.