TWI644124B - Sample adhesive element, sample carrying module and portable microscope apparatus using the same - Google Patents
Sample adhesive element, sample carrying module and portable microscope apparatus using the same Download PDFInfo
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- TWI644124B TWI644124B TW106102209A TW106102209A TWI644124B TW I644124 B TWI644124 B TW I644124B TW 106102209 A TW106102209 A TW 106102209A TW 106102209 A TW106102209 A TW 106102209A TW I644124 B TWI644124 B TW I644124B
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- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/34—Microscope slides, e.g. mounting specimens on microscope slides
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- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/02—Objectives
- G02B21/04—Objectives involving mirrors
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- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/0004—Microscopes specially adapted for specific applications
- G02B21/0008—Microscopes having a simple construction, e.g. portable microscopes
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- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/06—Means for illuminating specimens
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- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/36—Microscopes arranged for photographic purposes or projection purposes or digital imaging or video purposes including associated control and data processing arrangements
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- Microscoopes, Condenser (AREA)
- Accessories Of Cameras (AREA)
- Sampling And Sample Adjustment (AREA)
- Optical Measuring Cells (AREA)
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Abstract
一種可攜式顯微鏡裝置,其與一影像擷取裝置搭配使用,可攜式顯微鏡裝置包括一樣品承載組件、一透鏡模組、一第一偏光片以及一第二偏光片。樣品承載組件包括一透光載體及一樣品黏著件。樣品黏著件具有一基材及一膠層。基材具有一凹部及一延伸部,延伸部鄰接於凹部以形成一第一表面。膠層至少部分設置於第一表面,並與基材結合為一體。透鏡模組可拆卸地連接於影像擷取裝置,並設置於樣品承載組件與影像擷取裝置之間,第一偏光片設置於樣品承載組件一側的光學路徑上,第二偏光片設置於樣品承載組件另一側的光學路徑上。 A portable microscope device is used in conjunction with an image capturing device. The portable microscope device includes a sample bearing component, a lens module, a first polarizer, and a second polarizer. The sample-bearing component includes a light-transmissive carrier and a sample adhesive. The sample adhesive has a substrate and an adhesive layer. The substrate has a concave portion and an extended portion, and the extended portion is adjacent to the concave portion to form a first surface. The adhesive layer is at least partially disposed on the first surface and is integrated with the substrate. The lens module is detachably connected to the image capturing device, and is disposed between the sample bearing component and the image capturing device. The first polarizer is arranged on the optical path on one side of the sample bearing component, and the second polarizer is arranged on the sample The optical path on the other side of the carrier assembly.
Description
本發明是關於一種應用於樣品承載組件及可攜式顯微鏡裝置的樣品黏著件,特別是關於一種具有凹部的樣品黏著件。 The invention relates to a sample adhesive member applied to a sample bearing assembly and a portable microscope device, and more particularly to a sample adhesive member having a concave portion.
顯微鏡常用於觀察微生物、植物或礦物的微觀結構,然而,顯微鏡樣品的製備過程較為繁瑣,通常需要先對待測樣品進行前置處理,以形成易透光的薄切片,再將切片放置於載玻片上,接著在切片上滴上少量的液體(通常是水),最後將蓋玻片放置於切片上進行封片,如此才算完成標準試片的製備。此外,傳統顯微鏡的體積較為龐大,故常被置放於實驗室內,且通常需透過專業人員進行操作。然而,若每次發現新的標的物時,皆必須採樣至實驗室後始能觀察,將造成使用者的不便,且難以應用在非專業的一般使用者。 Microscopes are often used to observe the microstructure of microorganisms, plants, or minerals. However, the preparation process of microscope samples is tedious. Usually, the samples to be tested need to be pre-processed to form thin slices that are easy to transmit light. On the slide, a small amount of liquid (usually water) is dropped on the slide, and finally the cover glass is placed on the slide to cover the slide, so that the preparation of the standard test slide is completed. In addition, the traditional microscope is relatively large, so it is often placed in a laboratory, and usually requires professional operations. However, every time a new target is found, it must be sampled in the laboratory before it can be observed, which will cause inconvenience to the user and is difficult to apply to non-professional general users.
因此,如何提供一種簡便的樣品製備方式及可裝設於手持拍攝裝置上的可攜式顯微鏡裝置,實為當前重要的課題之一。 Therefore, how to provide a simple sample preparation method and a portable microscope device that can be mounted on a handheld photographing device is really one of the important issues at present.
本發明之一目的為提供一種樣品黏著件及樣品承載組件,可簡化顯微鏡樣品的製備流程。 An object of the present invention is to provide a sample adhesive member and a sample carrying component, which can simplify the preparation process of a microscope sample.
本發明之另一目的為提供一種可攜式顯微鏡裝置,可搭配影像擷取裝置使用,以拍攝微小物品的顯微結構。 Another object of the present invention is to provide a portable microscope device that can be used with an image capturing device to photograph the microstructure of a small object.
為達上述目的及其他目的,本發明提供一種樣品黏著件,包括一基材以及一膠層。基材具有一凹部及一延伸部,延伸部鄰接於凹部以形成一第一表面。膠層至少部分設置於第一表面,並與基材結合為一體。 In order to achieve the above-mentioned object and other objects, the present invention provides a sample adhesive member including a substrate and an adhesive layer. The substrate has a concave portion and an extended portion, and the extended portion is adjacent to the concave portion to form a first surface. The adhesive layer is at least partially disposed on the first surface and is integrated with the substrate.
在一實施例中,膠層位於凹部的黏性低於膠層位於延伸部的黏性。 In one embodiment, the adhesive layer has a lower viscosity than the adhesive layer at the recess.
在一實施例中,樣品黏著件至少有部分區域為透光區。 In one embodiment, at least a part of the region of the sample adhesive is a light transmitting region.
在一實施例中,延伸部為不透光區,凹部為透光區。 In one embodiment, the extending portion is an opaque area and the recessed portion is a light transmitting area.
在一實施例中,凹部具有一遮光點。 In one embodiment, the concave portion has a light shielding point.
在一實施例中,樣品黏著件為貼紙。 In one embodiment, the sample adhesive is a sticker.
為達上述目的及其他目的,本發明提供一種樣品承載組件,包括一透光載體以及一前述的樣品黏著件。樣品黏著件具有一凹部,其中樣品黏著件可移除地黏設於透光載體的一表面,使凹部與表面形成一容置空間。 To achieve the above and other objectives, the present invention provides a sample carrying assembly, which includes a light-transmissive carrier and the aforementioned sample adhesive. The sample adhesive has a concave portion, and the sample adhesive is removably adhered to a surface of the light-transmitting carrier, so that the concave portion and the surface form an accommodating space.
在一實施例中,凹部沾黏一待測樣品後,延伸部與透光載體相貼合,以使待測樣品封閉於容置空間。 In one embodiment, after the concave portion adheres to a sample to be tested, the extension portion is adhered to the light-transmitting carrier so that the sample to be tested is enclosed in the accommodating space.
在一實施例中,容置空間可防止液體外漏。 In one embodiment, the accommodating space prevents leakage of liquid.
為達上述目的及其他目的,本發明提供一種可攜式顯微鏡裝置,其與一影像擷取裝置搭配使用。可攜式顯微鏡裝置包括一前述的樣品承載組件以及一透鏡模組。透鏡模組可拆卸地連接於影像擷取裝置,並設置於樣品承載組件與影像擷取裝置之間。 To achieve the above and other objects, the present invention provides a portable microscope device for use with an image capture device. The portable microscope device includes the aforementioned sample bearing assembly and a lens module. The lens module is detachably connected to the image capturing device, and is disposed between the sample bearing component and the image capturing device.
在一實施例中,可攜式顯微鏡裝置更包括一光源模組,包括一座體及一發光元件,發光元件設置於座體內。 In one embodiment, the portable microscope device further includes a light source module, including a base and a light emitting element, and the light emitting element is disposed in the base.
在一實施例中,透鏡模組設置有一第一磁性元件,座體設置有一第二磁性元件,當透鏡模組磁性吸附於座體時,發光元件被啟用而發光。 In one embodiment, the lens module is provided with a first magnetic element, and the base body is provided with a second magnetic element. When the lens module is magnetically adsorbed on the base body, the light emitting element is activated to emit light.
在一實施例中,透鏡模組設置有一磁性元件且座體具有金屬殼體,或座體設置有一磁性元件且透鏡模組具有金屬殼體,當透鏡模組磁性吸附於座體時,發光元件被啟用而發光。 In an embodiment, the lens module is provided with a magnetic element and the base body has a metal shell, or the base body is provided with a magnetic element and the lens module has a metal shell. When the lens module is magnetically attached to the base body, the light emitting element is Enable and glow.
在一實施例中,座體更設置有一限位部,樣品承載組件可移動地設置於座體上,並與限位部相抵接。 In one embodiment, the base body is further provided with a limiting portion, and the sample bearing component is movably disposed on the base body and abuts the limiting portion.
在一實施例中,可攜式顯微鏡裝置更包括一蓋體,蓋體可拆卸地卡合於限位部。 In one embodiment, the portable microscope device further includes a cover, and the cover is detachably engaged with the limiting portion.
在一實施例中,可攜式顯微鏡裝置更包括一組固定件,用以將透鏡模組固定於影像擷取裝置。 In one embodiment, the portable microscope device further includes a set of fixing members for fixing the lens module to the image capturing device.
在一實施例中,可攜式顯微鏡裝置更包括一第一偏光片以及一第二偏光片。第一偏光片設置於樣品承載組件一側的光學路徑上,第二偏光片設置於樣品承載組件另一側的光學路徑上。 In one embodiment, the portable microscope device further includes a first polarizer and a second polarizer. The first polarizer is disposed on the optical path on one side of the sample carrying component, and the second polarizer is disposed on the optical path on the other side of the sample carrying component.
為達上述目的及其他目的,本發明提供一種可攜式顯微鏡裝置,其與一影像擷取模組搭配使用。可攜式顯微鏡裝置包括一殼體、一凸透鏡、一光源模組以及一前述的樣品承載組件,殼體具有一樣品觀察面,樣品觀察面位於殼體相對於影像擷取模組之一側。凸透鏡設置於殼體內部,樣品觀察面至凸透鏡的最短距離介於0.1mm至10.0mm之間,光源模組設置於影像擷取模組與凸透鏡之間。 To achieve the above and other objectives, the present invention provides a portable microscope device that is used in conjunction with an image capture module. The portable microscope device includes a casing, a convex lens, a light source module, and the aforementioned sample bearing assembly. The casing has a sample observation surface, and the sample observation surface is located on one side of the casing relative to the image capture module. The convex lens is arranged inside the housing, the shortest distance from the sample observation surface to the convex lens is between 0.1mm and 10.0mm, and the light source module is arranged between the image capturing module and the convex lens.
在一實施例中,樣品觀察面至凸透鏡的最短距離介於0.1mm至3.0mm之間。 In one embodiment, the shortest distance between the sample observation surface and the convex lens is between 0.1 mm and 3.0 mm.
在一實施例中,凸透鏡具有一翼部,翼部位於凸透鏡之週緣。 In one embodiment, the convex lens has a wing portion, and the wing portion is located on the periphery of the convex lens.
在一實施例中,可攜式顯微鏡裝置更包括一連接元件,連接元件設置於殼體,以連接於影像擷取模組。 In one embodiment, the portable microscope device further includes a connecting element, and the connecting element is disposed on the casing to be connected to the image capturing module.
承上所述,本發明是藉由樣品黏著件的凹部沾黏一待測樣品,並貼附於透光載體上,即完成顯微鏡樣品的製備,因此大幅簡化了傳統顯微鏡樣品的製備流程。此外,本發明的樣品黏著件採用沖壓凹部的設計方式,不僅可容置液態樣品或活體樣品,也有助於封閉待測樣品,並能防止液體外漏,故具有較佳的密封效果。再者,本發明的可攜式顯微鏡裝置可輕易裝設於影像擷取裝置上,以拍攝微小物品的顯微結構,並可搭配一組偏光片,以觀察待測樣品內部不同相的微觀結構,故可達成提升成像解析度及方便攜帶的功效。 As mentioned above, the present invention completes the preparation of a microscope sample by sticking a sample to be tested by attaching a concave portion of a sample adhesive member and attaching the sample to a light-transmissive carrier, thereby greatly simplifying a traditional microscope sample preparation process. In addition, the sample adhesive part of the present invention adopts the design of the stamped recess, which can not only accommodate liquid samples or living samples, but also help to seal the sample to be tested, and can prevent liquid leakage, so it has a better sealing effect. Furthermore, the portable microscope device of the present invention can be easily installed on an image capture device to photograph the microstructure of a small object, and can be used with a set of polarizers to observe the microstructure of different phases inside the sample to be measured Therefore, it can achieve the effect of improving imaging resolution and portability.
7、100‧‧‧可攜式顯微鏡裝置 7, 100‧‧‧ portable microscope device
10‧‧‧樣品承載組件 10‧‧‧Sample carrier
11‧‧‧透光載體 11‧‧‧ transparent carrier
12、12a、12b‧‧‧樣品黏著件 12, 12a, 12b ‧‧‧ Sample Adhesive Parts
13‧‧‧容置空間 13‧‧‧accommodation space
20、73‧‧‧光源模組 20, 73‧‧‧light source module
21‧‧‧座體 21‧‧‧ seat
22、731‧‧‧發光元件 22, 731‧‧‧light-emitting elements
23‧‧‧開關 23‧‧‧Switch
24‧‧‧第二磁性元件 24‧‧‧Second magnetic element
30‧‧‧透鏡模組 30‧‧‧ lens module
31‧‧‧顯微鏡頭 31‧‧‧ microscope head
32‧‧‧第一磁性元件 32‧‧‧first magnetic element
33‧‧‧固定件 33‧‧‧Fixed parts
40‧‧‧第一偏光片 40‧‧‧first polarizer
41‧‧‧第二偏光片 41‧‧‧Second polarizer
50‧‧‧蓋體 50‧‧‧ Cover
51‧‧‧本體 51‧‧‧ Ontology
60‧‧‧影像擷取裝置 60‧‧‧Image capture device
61‧‧‧影像擷取模組 61‧‧‧Image capture module
71‧‧‧殼體 71‧‧‧shell
72‧‧‧凸透鏡 72‧‧‧ convex lens
75‧‧‧連接元件 75‧‧‧Connecting element
111‧‧‧樣品承載面 111‧‧‧sample bearing surface
120‧‧‧基材 120‧‧‧ substrate
121、121a、121b‧‧‧延伸部 121, 121a, 121b‧‧‧ extension
122、122a、122b‧‧‧凹部 122, 122a, 122b ‧‧‧ recess
123‧‧‧遮光點 123‧‧‧Shading Point
211、712‧‧‧出光孔 211, 712‧‧‧light exit hole
212‧‧‧限位部 212‧‧‧Limiting Department
331‧‧‧公次件 331‧‧‧Public
332‧‧‧母次件 332‧‧‧Parent
511‧‧‧通光孔 511‧‧‧light hole
512‧‧‧凸部 512‧‧‧ convex
514‧‧‧卡扣部 514‧‧‧Snap
711‧‧‧樣品觀察面 711‧‧‧Sample observation surface
713‧‧‧開口 713‧‧‧ opening
721‧‧‧翼部 721‧‧‧wing
722‧‧‧第一凸面 722‧‧‧first convex
723‧‧‧第二凸面 723‧‧‧Second Convex
1201‧‧‧第二表面 1201‧‧‧Second surface
1202‧‧‧第一表面 1202‧‧‧First surface
1203‧‧‧膠層 1203‧‧‧ Adhesive layer
D‧‧‧最短距離 D‧‧‧ shortest distance
S‧‧‧待測樣品 S‧‧‧Test sample
d1‧‧‧直徑 d1‧‧‧ diameter
d2‧‧‧孔徑 d2‧‧‧ aperture
圖1A為依據本發明一實施例的樣品黏著件的細部結構示意圖。 FIG. 1A is a detailed structure diagram of a sample adhesive member according to an embodiment of the present invention.
圖1B為依據本發明一實施例的樣品承載組件的外觀示意圖。 FIG. 1B is a schematic diagram of an appearance of a sample bearing assembly according to an embodiment of the present invention.
圖1C為圖1B沿AA切線的剖面示意圖。 FIG. 1C is a schematic cross-sectional view taken along the line AA of FIG. 1B.
圖2A-2B為樣品黏著件凹部的變化態樣的剖面示意圖。 Figures 2A-2B are schematic cross-sectional views of a modified state of a concave portion of a sample adhesive member.
圖3A-3B為依據本發明一實施例的樣品黏著件的俯視示意圖。 3A-3B are schematic top views of a sample adhesive member according to an embodiment of the present invention.
圖4A為依據本發明一實施例的可攜式顯微鏡裝置的分解結構示意圖。 FIG. 4A is a schematic exploded view of a portable microscope device according to an embodiment of the present invention.
圖4B為圖4A的可攜式顯微鏡裝置的組裝示意圖。 FIG. 4B is an assembly diagram of the portable microscope device of FIG. 4A.
圖5A為依據本發明一實施例的可攜式顯微鏡裝置搭配影像擷取裝置使用的示意圖。 FIG. 5A is a schematic diagram of using a portable microscope device with an image capturing device according to an embodiment of the present invention.
圖5B為依據本發明一實施例的可攜式顯微鏡裝置組裝於影像擷取裝置的示意圖。 FIG. 5B is a schematic diagram of assembling a portable microscope device to an image capturing device according to an embodiment of the present invention.
圖6為依據本發明另一實施例的可攜式顯微鏡裝置組裝於影像擷取裝置的示意圖。 FIG. 6 is a schematic diagram of a portable microscope device assembled in an image capturing device according to another embodiment of the present invention.
圖7A至圖7D為圖6中可攜式顯微鏡裝置的剖面示意圖。 7A to 7D are schematic cross-sectional views of the portable microscope device in FIG. 6.
以下將參照相關圖式,說明依據本發明具體實施例的顯示屏幕的貼合裝置,其中相同的元件將以相同的元件符號加以說明,所附圖式僅為說明用途,並非用於侷限本發明。 The following will describe the laminating device for a display screen according to a specific embodiment of the present invention with reference to the related drawings. The same components will be described with the same component symbols. The drawings are for illustrative purposes only and are not intended to limit the present invention. .
關於本文中所使用之「連接」的用詞,除了包括元件與元件直接的相連,亦包括元件與元件間接的相連,例如二元件之間可能另包括介質或其他元件。其中,部分已知的元件可能會省略以避免模糊本發明的概念。 With regard to the term "connected" as used herein, in addition to the direct connection of elements to components, it also includes the indirect connection of elements to elements. For example, two elements may include media or other elements. Among them, some known elements may be omitted to avoid obscuring the concept of the present invention.
圖1A為依據本發明一實施例的樣品黏著件的細部結構示意圖。圖1B為依據本發明一實施例的樣品承載組件的外觀示意圖。圖1C為圖1B沿AA切線的剖面示意圖。請同時參閱圖1A及圖1C所示,樣品黏著件12包括一基材120以及一膠層1203。基材120具有相對的一第二表面1201與一第一表面1202,膠層1203設置於基材120的第一表面1202,並與基材120結合為一體。基材120具有一延伸部121及一凹部122,延伸部121鄰接於凹部122。膠層1203位於凹部122的黏性低於膠層1203位於延伸部121的黏性。此外,本實施例的基材120及膠層1203係可透光,其透 光率係大於90%。 FIG. 1A is a detailed structure diagram of a sample adhesive member according to an embodiment of the present invention. FIG. 1B is a schematic diagram of an appearance of a sample bearing assembly according to an embodiment of the present invention. FIG. 1C is a schematic cross-sectional view taken along the line AA of FIG. 1B. Please refer to FIG. 1A and FIG. 1C together. The sample adhesive 12 includes a substrate 120 and an adhesive layer 1203. The substrate 120 has a second surface 1201 and a first surface 1202 opposite to each other. The adhesive layer 1203 is disposed on the first surface 1202 of the substrate 120 and is integrated with the substrate 120 as a whole. The base material 120 has an extending portion 121 and a concave portion 122. The extending portion 121 is adjacent to the concave portion 122. The adhesiveness of the adhesive layer 1203 at the concave portion 122 is lower than the adhesiveness of the adhesive layer 1203 at the extending portion 121. In addition, the substrate 120 and the adhesive layer 1203 in this embodiment are transparent, and the transparent The luminosity is greater than 90%.
請同時參閱圖1B-1C所示,樣品承載組件10,包括:一透光載體11以及一樣品黏著件12。透光載體11具有一樣品承載面111,透光載體11可以是載玻片、塑膠片、或樹脂片。樣品黏著件12位於樣品承載面111的一側具有黏性,當樣品黏著件12的延伸部121貼附於透光載體11時,凹部122與樣品承載面111可形成一容置空間13,以盛裝一待測樣品S。使用者只需藉由樣品黏著件12的凹部122容置或沾黏待測樣品S,並貼附於透光載體11上,即完成顯微鏡樣品的製備,因此大幅簡化了傳統顯微鏡樣品的製備流程。 Please also refer to FIGS. 1B-1C. The sample carrying assembly 10 includes a transparent carrier 11 and a sample adhesive 12. The transparent carrier 11 has a sample carrying surface 111. The transparent carrier 11 may be a glass slide, a plastic sheet, or a resin sheet. The sample adhesive 12 is located on one side of the sample bearing surface 111. When the extension 121 of the sample adhesive 12 is attached to the light-transmitting carrier 11, the recess 122 and the sample bearing surface 111 can form an accommodating space 13. Hold a sample S to be tested. The user only needs to hold or stick the sample S to be tested by the recess 122 of the sample adhesive 12 and attach it to the light-transmissive carrier 11 to complete the preparation of the microscope sample, thus greatly simplifying the preparation process of the traditional microscope sample. .
容置空間13可封閉一待測樣品S,並能防止液體外漏。待測樣品S可以是微生物、細胞、節肢動物或礦物粉末…等等。此外,待測樣品S並不限於液態樣品或活體樣品。例如:使用者可藉由樣品黏著件12的凹部122沾黏活的昆蟲或承裝液態檢體,再將樣品黏著件12的延伸部121貼附於透光載體11的樣品承載面111,以將活的昆蟲或液態檢體密封於容置空間13。由於凹部12的黏性低於延伸部121的黏性,因此較小的活體昆蟲仍可在容置空間13內活動,而不至於被樣品黏著件12壓死。若使用者想要保存待測樣品S時,可從透光載體11上將樣品黏著件12撕除,並貼附於另一塑膠膜或貼紙簿,以做為收藏之用。 The accommodating space 13 can close a sample S to be measured, and can prevent the liquid from leaking out. The test sample S can be a microorganism, a cell, an arthropod or a mineral powder ... and so on. In addition, the test sample S is not limited to a liquid sample or a living sample. For example, the user can stick the live insect or the liquid specimen through the recess 122 of the sample adhesive 12, and then attach the extension 121 of the sample adhesive 12 to the sample bearing surface 111 of the transparent carrier 11 to A living insect or liquid specimen is sealed in the accommodation space 13. Since the viscosity of the recessed portion 12 is lower than that of the extension portion 121, a smaller living insect can still move in the accommodation space 13 without being crushed to death by the sample adhesive 12. If the user wants to store the sample S to be tested, the sample adhesive 12 can be removed from the transparent carrier 11 and attached to another plastic film or sticker for storage.
圖2A-2B為樣品黏著件凹部的變化態樣的剖面示意圖。請同時參閱圖1C及圖2A-2B所示,樣品黏著件12、12a、12b分別具有一延伸部121、121a、121b及一凹部122、122a、122b。樣品黏著件12、12a、12b可以是貼紙、膠帶、膠膜或是樹脂,並具有延展性。因此,使用者可藉由沖壓成形的方式,將樣品黏著件沖壓出不同形狀的內凹結構。例如:圖1C的凹部122及圖2A的凹部122a為不同類型的弧形結構,圖2B的凹部122b則為一方形結構。此外,樣品黏著件12、12a、12b亦可依需求沖壓出不同厚度的內凹結構,或是將凹部122、122a、122b印刷成不同的顏色。 Figures 2A-2B are schematic cross-sectional views of a modified state of a concave portion of a sample adhesive member. Please refer to FIG. 1C and FIG. 2A-2B at the same time. The sample adhesives 12, 12a, and 12b respectively have an extension 121, 121 a, 121 b and a recess 122, 122 a, 122 b. The sample adhesive pieces 12, 12a, 12b can be stickers, tapes, films, or resins, and have ductility. Therefore, the user can punch the sample adhesive parts into different shapes of concave structures by means of stamping. For example, the concave portion 122 of FIG. 1C and the concave portion 122a of FIG. 2A have different types of arc structures, and the concave portion 122b of FIG. 2B has a square structure. In addition, the sample adhesive pieces 12, 12a, and 12b can also be punched out into concave structures of different thicknesses, or the concave portions 122, 122a, and 122b can be printed in different colors as required.
以下實施例中,樣品黏著件12將以貼紙為例進行說明。樣品黏著件12可為一透光貼紙,其透光率係大於90%,亦可於樣品黏著件12的部分區域做遮光處理,以形成一暗場貼紙。 In the following embodiments, the sample adhesive member 12 will be described using a sticker as an example. The sample adhesive member 12 may be a light-transmitting sticker, and the light transmittance thereof is greater than 90%. A light-shielding treatment may also be performed on a part of the sample adhesive member 12 to form a dark-field sticker.
圖3A至圖3B為依據本發明實施例的樣品黏著件的俯視示意圖。請參閱圖3A所示,樣品黏著件12的凹部122具有一遮光點123,遮光點123外的區域均為透光區。於圖3A的實施態樣中,遮光點123為一印刷黑點,遮光點123可設置於凹部122表面的任何位置,不一定要設置於凹部122的中心位置,只要待測樣品S的成像背景呈現出暗場的效果即可。圖3A的凹部122亦可設置多個分散的印刷黑點,以形成遮光區域。因此,僅需將一個或多個分散的印刷黑點設置於原本整體透光的凹部122,以形成遮光區域,儘量只讓折射和散射的光線射至待測樣品S,即可達到暗場的效果,並提升解析度。 3A to 3B are schematic top views of a sample adhesive member according to an embodiment of the present invention. Please refer to FIG. 3A, the concave portion 122 of the sample adhesive 12 has a light-shielding point 123, and areas outside the light-shielding point 123 are light-transmitting areas. In the embodiment of FIG. 3A, the light-shielding point 123 is a printing black point, and the light-shielding point 123 may be set at any position on the surface of the recessed portion 122. Shows the effect of the dark field. The concave portion 122 of FIG. 3A may also be provided with a plurality of scattered printed black dots to form a light-shielding region. Therefore, it is only necessary to set one or more scattered printed black dots on the recessed portion 122 that is originally transparent, so as to form a light-shielding area. Try to allow only the refracted and scattered light to reach the sample S to be measured, and the dark field can be achieved. Effect and increase the resolution.
於另一實施態樣中,如圖3B所示,樣品黏著件12僅有凹部122為透光區,其餘區域均為不透光區。因此,僅需將樣品黏著件12的非凹部區域設置為遮光區域,即可讓樣品黏著件12成為一暗場貼紙。此外,圖3A與圖3B的態樣亦可結合使用。藉由暗場貼紙的設計方式,可提高樣品本身與背景之間的對比,進而獲得較佳的成像效果。 In another embodiment, as shown in FIG. 3B, only the concave portion 122 of the sample adhesive 12 is a light-transmitting area, and the remaining areas are opaque areas. Therefore, it is only necessary to set the non-concave region of the sample adhesive 12 as a light-shielding region, so that the sample adhesive 12 becomes a dark field sticker. In addition, the aspects of FIG. 3A and FIG. 3B can also be used in combination. Through the design of the dark field sticker, the contrast between the sample itself and the background can be improved, and a better imaging effect can be obtained.
圖4A為依據本發明一實施例的可攜式顯微鏡裝置的分解結構示意圖。圖4B為圖4A的可攜式顯微鏡裝置的組裝示意圖。請參閱圖4A所示,可攜式顯微鏡裝置100包括前述的樣品承載組件10、一透鏡模組30、一蓋體50以及一光源模組20。樣品承載組件10包括透光載體11及樣品黏著件12。光源模組20包括一座體21、一發光元件22及一開關23。座體21內係呈中空狀,可容置發光元件22及其驅動電路,發光元件22可為燈泡或發光二極體,並耦接於其驅動電路及開關23。座體21具有一出光孔211及一限位部212。蓋體50包括一本體51、一凸部512及一卡扣部514。本體51具有一通光孔511,凸部512及卡扣部514位於本體51的同一側。蓋體50的卡扣部514可與座體21的限位部212互相卡合,以供蓋體50可拆卸地設置於座體21。 FIG. 4A is a schematic exploded view of a portable microscope device according to an embodiment of the present invention. FIG. 4B is an assembly diagram of the portable microscope device of FIG. 4A. Please refer to FIG. 4A, the portable microscope device 100 includes the aforementioned sample carrying assembly 10, a lens module 30, a cover 50 and a light source module 20. The sample carrying assembly 10 includes a light-transmissive carrier 11 and a sample adhesive 12. The light source module 20 includes a base 21, a light emitting element 22 and a switch 23. The base 21 has a hollow shape and can accommodate a light emitting element 22 and a driving circuit thereof. The light emitting element 22 may be a light bulb or a light emitting diode, and is coupled to the driving circuit and the switch 23. The base 21 has a light emitting hole 211 and a limiting portion 212. The cover 50 includes a main body 51, a convex portion 512 and a buckling portion 514. The main body 51 has a light through hole 511, and the convex portion 512 and the latching portion 514 are located on the same side of the main body 51. The latching portion 514 of the cover 50 and the limiting portion 212 of the base 21 can be engaged with each other, so that the cover 50 can be detachably disposed on the base 21.
如圖4A所示,透鏡模組30鑲設有一顯微鏡頭31,且透鏡模組30底部設置有一第一磁性元件32,座體21設置有一第二磁性元件24。第一磁性元件32及第二磁性元件24可採用具有永久磁性的物質,例如永久磁鐵或強力磁鐵。本實施例中,透鏡模組30及座體21分別設置有第一 磁性元件32及第二磁性元件24,當透鏡模組30或座體21其中之一具有金屬殼體時,則不需同時設置磁性元件於透鏡模組30及座體21上。例如:當透鏡模組30具有金屬殼體時,則僅需設置第二磁性元件24於座體21上,而當座體21具有金屬殼體時,則僅需設置第一磁性元件32於透鏡模組30上。因此,透鏡模組30與座體21可藉由磁力作用相互吸附。 As shown in FIG. 4A, the lens module 30 is inlaid with a microscope head 31, a first magnetic element 32 is disposed at the bottom of the lens module 30, and a second magnetic element 24 is disposed at the base 21. The first magnetic element 32 and the second magnetic element 24 may be made of a substance having permanent magnetism, such as a permanent magnet or a strong magnet. In this embodiment, the lens module 30 and the base 21 are respectively provided with a first For the magnetic element 32 and the second magnetic element 24, when one of the lens module 30 or the base 21 has a metal case, it is not necessary to provide the magnetic element on the lens module 30 and the base 21 at the same time. For example: when the lens module 30 has a metal case, only the second magnetic element 24 needs to be provided on the base 21, and when the base 21 has a metal case, only the first magnetic element 32 needs to be provided on the lens Module 30. Therefore, the lens module 30 and the base 21 can be attracted to each other by a magnetic force.
請參閱圖4B所示,樣品承載組件10容置有一待測樣品S,並與座體21的限位部212相抵接,使用者可沿限位部212的軸向方向移動樣品承載組件10,以調整待測樣品S的觀察位置。蓋體50設置於樣品承載組件10上,並與限位部212互相卡合,當透鏡模組30裝設於蓋體50上時,透鏡模組30與座體21藉由磁力作用相吸附,而將透鏡模組30固定於座體21,此時蓋體50的凸部512因受到一向下壓力,而壓迫透光載體11按壓開關23。由於開關23電性連接發光元件22的驅動電路,故當透鏡模組30固定於座體21時,開關23因受透光載體11壓迫而被按下,致使發光元件22被驅動而發光。 Please refer to FIG. 4B, the sample carrying assembly 10 contains a sample S to be tested and abuts against the limiting portion 212 of the base 21. The user can move the sample carrying assembly 10 in the axial direction of the limiting portion 212. In order to adjust the observation position of the sample S to be measured. The cover body 50 is disposed on the sample bearing assembly 10 and engages with the limiting portion 212. When the lens module 30 is installed on the cover body 50, the lens module 30 and the base body 21 are attracted by magnetic force. The lens module 30 is fixed to the base 21. At this time, the convex portion 512 of the cover 50 receives a downward pressure and presses the transparent carrier 11 to press the switch 23. Since the switch 23 is electrically connected to the driving circuit of the light-emitting element 22, when the lens module 30 is fixed to the base 21, the switch 23 is pressed by being pressed by the light-transmitting carrier 11, so that the light-emitting element 22 is driven to emit light.
顯微鏡頭31可藉由蓋體50之厚度與待測樣品S間隔一適當距離,使待測樣品S位於顯微鏡頭31之成像聚焦平面上,故顯微鏡頭31與待測樣品S間可以維持最佳化的焦距。發光元件22所發出的光可以是可見光或非可見光,例如:發光元件22可產生一紅外光或一紫外光。舉例來說,若發光元件22所發出的光為紫外光時,可攜式顯微鏡裝置100便可成為螢光檢測器,以應用於生物細胞檢體的計數。 The microscope head 31 can be spaced at an appropriate distance from the sample S to be measured by the thickness of the cover 50, so that the sample S to be measured is located on the imaging focal plane of the microscope head 31. Therefore, the best relationship can be maintained between the microscope head 31 and the sample S to be measured Focal length. The light emitted by the light emitting element 22 may be visible light or non-visible light. For example, the light emitting element 22 may generate an infrared light or an ultraviolet light. For example, if the light emitted from the light-emitting element 22 is ultraviolet light, the portable microscope device 100 can become a fluorescent detector for application in counting biological cell specimens.
圖5A為依據本發明一實施例的可攜式顯微鏡裝置搭配影像擷取裝置使用的示意圖。請參閱圖5A所示,可攜式顯微鏡裝置100可與一影像擷取裝置60搭配使用。影像擷取裝置60可以是智慧型手機、平板電腦、筆記型電腦、行車記錄器、相機、自拍器或穿戴式電子裝置。以下實施例的影像擷取裝置60將以智慧型手機為例進行說明。本發明的可攜式顯微鏡裝置100搭配影像擷取裝置60操作時,可藉由夾具、磁鐵、吸盤或卡榫等方式,將透鏡模組30裝設於影像擷取裝置60的影像擷取模組61上。使用者可藉由樣品黏著件12的凹部沾黏一待測樣品S,並貼附於透光載體11上,例如:可貼附於載玻片上。接著,使用者僅需手持樣品承載組件10, 並手動調整待測樣品S與顯微鏡頭31間的距離,即可藉由影像擷取裝置60觀察或拍攝待測樣品S的微觀結構。此外,當透鏡模組30具有一透光殼體時,使用者可直接將黏附有待測樣品S的樣品黏著件12貼附於透鏡模組30的透光殼體,而省略載玻片的使用。 FIG. 5A is a schematic diagram of using a portable microscope device with an image capturing device according to an embodiment of the present invention. Please refer to FIG. 5A, the portable microscope device 100 can be used with an image capturing device 60. The image capturing device 60 may be a smart phone, a tablet computer, a notebook computer, a driving recorder, a camera, a self-timer, or a wearable electronic device. The image capturing device 60 in the following embodiments will be described by taking a smart phone as an example. When the portable microscope device 100 of the present invention is operated with the image capture device 60, the lens module 30 can be mounted on the image capture module of the image capture device 60 by means of a clamp, a magnet, a suction cup, or a tenon. Group 61 on. The user can stick a sample S to be tested through the concave portion of the sample adhesive 12 and attach it to the light-transmissive carrier 11, for example, it can be attached to a glass slide. Then, the user only needs to hold the sample carrying assembly 10, And by manually adjusting the distance between the sample S to be measured and the microscope head 31, the microstructure of the sample S to be measured can be observed or photographed by the image capturing device 60. In addition, when the lens module 30 has a transparent case, the user can directly attach the sample adhesive member 12 to which the sample S to be tested is attached to the transparent case of the lens module 30, and omit the slide glass. use.
如圖5A所示,可攜式顯微鏡裝置100可配置一第一偏光片40及一第二偏光片41,並相對設置於樣品承載組件10兩側的光學路徑上,以觀察待測樣品S中不同相之結構與型態。例如:第一偏光片40可裝設於透鏡模組30上,或直接黏設於影像擷取模組61上,第二偏光片41可黏設於樣品黏著件12上,並對應於待測樣品S的位置。使用者可藉由旋轉樣品承載組件10,以改變第一偏光片40及第二偏光片41偏振軸的相對角度,使不同偏振角度的光通過待測樣品S,藉此可觀察待測樣品S中不同相之結構與型態,例如:在偏光模式下,不需經由染色,即可清楚觀察到澱粉結晶的微觀結構。 As shown in FIG. 5A, the portable microscope device 100 can be configured with a first polarizer 40 and a second polarizer 41, and are disposed on the optical paths on both sides of the sample carrier 10 to observe the sample S to be measured. Structure and type of different phases. For example, the first polarizer 40 may be mounted on the lens module 30 or directly adhered to the image capturing module 61, and the second polarizer 41 may be adhered to the sample adhesive 12 and corresponds to the test object. Location of sample S. The user can rotate the sample carrier 10 to change the relative angles of the polarization axes of the first polarizer 40 and the second polarizer 41 so that light with different polarization angles can pass through the sample S to be observed, thereby observing the sample S to be measured. The structure and type of the different phases in the medium, for example: in polarized light mode, the microstructure of starch crystals can be clearly observed without dyeing.
圖5B為依據本發明一實施例的可攜式顯微鏡裝置組裝於影像擷取裝置的的示意圖。如圖5B所示,可攜式顯微鏡裝置100可裝設一組固定件33,以將透鏡模組30固定於影像擷取裝置60。固定件33亦可整合於影像擷取裝置60的背蓋或保護殼中。於本實施例中,固定件33具有一公次件331設置於透鏡模組30,及一母次件332對應設置於影像擷取模組61。公次件331及母次件332分別具有互相匹配之螺紋,透過彼此螺接的方式,即可將透鏡模組30組裝於影像擷取模組61上。固定件33亦可以是黏著劑、夾具、磁鐵、吸盤或卡榫,本發明並未對透鏡模組30的固定方式加以限制。 FIG. 5B is a schematic diagram of a portable microscope device assembled in an image capturing device according to an embodiment of the present invention. As shown in FIG. 5B, the portable microscope device 100 may be provided with a set of fixing members 33 to fix the lens module 30 to the image capturing device 60. The fixing member 33 can also be integrated into a back cover or a protective case of the image capturing device 60. In this embodiment, the fixing member 33 has a male and female member 331 disposed on the lens module 30, and a female and male member 332 correspondingly disposed on the image capture module 61. The male and female parts 331 and the female and female parts 332 respectively have mutually matched threads. The lens module 30 can be assembled on the image capturing module 61 by screwing to each other. The fixing member 33 may also be an adhesive, a clamp, a magnet, a suction cup, or a tenon. The present invention does not limit the fixing manner of the lens module 30.
當透鏡模組30固定於影像擷取模組61後,使用者可先將圖5A中的樣品承載組件10夾設於蓋體50與座體21之間,以形成一整體構件,再將該整體構件裝設於透鏡模組30上,或先透過圖4B的方式組裝為可攜式顯微鏡裝置100,再將可攜式顯微鏡裝置100固定於影像擷取模組61。當使用者欲拍攝待測樣品S的成像時,可藉由發光元件22所投射出的光線照射待測樣品S,再經由顯微鏡頭31使待測樣品S的成像呈現於影像擷取裝置60的螢幕中,使用者便可對螢幕中的影像進行拍照。若待測樣品 S無法在固定的拍攝位置上得到全體成像時,可將座體21與影像擷取裝置60相對移動,以調整待測樣品S的成像位置。 After the lens module 30 is fixed to the image capturing module 61, the user can first sandwich the sample carrier assembly 10 in FIG. 5A between the cover 50 and the base 21 to form an integral component, and then The whole component is installed on the lens module 30 or assembled into a portable microscope device 100 through the method of FIG. 4B, and then the portable microscope device 100 is fixed to the image capturing module 61. When the user wants to take an image of the sample S to be measured, the sample S can be illuminated by the light projected from the light emitting element 22, and then the image of the sample S to be measured is presented to the image capturing device 60 through the microscope head 31. On the screen, users can take pictures of the images on the screen. If the sample to be tested When S cannot obtain the entire imaging at a fixed shooting position, the base 21 and the image capturing device 60 can be relatively moved to adjust the imaging position of the sample S to be measured.
請參閱圖5B所示,可攜式顯微鏡裝置100可加裝一第一偏光片40及一第二偏光片41,並相對設置於待測樣品S兩側的光學路徑上。例如:第一偏光片40可設置於待測樣品S與影像擷取模組61之間的光學路徑上,第二偏光片41可設置於待測樣品S與光源模組20之間的光學路徑上。於本實施例中,第一偏光片40是設置於透鏡模組30與影像擷取模組61之間,故可先將第一偏光片40裝設於透鏡模組30上再一併固定於影像擷取模組61,亦可將第一偏光片40直接貼附於影像擷取模組61上,再裝設透鏡模組30。第二偏光片41則設置於樣品承載組件10與光源模組20之間,例如:第二偏光片41可貼附於出光孔211處或貼附於樣品承載組件10靠近出光孔211的一側。第一偏光片40及第二偏光片41可為線性偏光片,於預設情況下,第一偏光片40及第二偏光片41的偏振軸方向係互相垂直。此外,第一偏光片40及第二偏光片410亦可為圓偏光片或楕圓偏光片。 Please refer to FIG. 5B. The portable microscope device 100 can be further equipped with a first polarizer 40 and a second polarizer 41, and are disposed on the optical paths on both sides of the sample S to be measured. For example, the first polarizer 40 may be disposed on the optical path between the sample S to be measured and the image capturing module 61, and the second polarizer 41 may be disposed on the optical path between the sample S to be measured and the light source module 20. on. In this embodiment, the first polarizer 40 is disposed between the lens module 30 and the image capturing module 61, so the first polarizer 40 may be installed on the lens module 30 and then fixed together. For the image capturing module 61, the first polarizer 40 can also be directly attached to the image capturing module 61, and then the lens module 30 is installed. The second polarizer 41 is disposed between the sample carrier 10 and the light source module 20, for example, the second polarizer 41 can be attached to the light exit hole 211 or a side of the sample carrier 10 near the light exit hole 211. . The first polarizer 40 and the second polarizer 41 may be linear polarizers. In a preset case, the polarization axis directions of the first polarizer 40 and the second polarizer 41 are perpendicular to each other. In addition, the first polarizer 40 and the second polarizer 410 may be a circular polarizer or a circular circular polarizer.
當光源模組20開啟時,若第一偏光片40及第二偏光片41的偏振軸方向係互相垂直,且樣品承載模組10承載了不具有雙折射性質的待測樣品S時,使用者將無法看到待測樣品S的成像。當樣品承載模組10承載了具有雙折射性質的待測樣品S時,發光元件22所發出的光線將依序通過第二偏光片41、待測樣品S、透鏡模組30及第一偏光片40,而到達影像擷取模組61,操作者即可透過影像擷取模組61拍攝待測樣品S的成像。此外,由於第一偏光片40係固定於影像擷取模組61上,操作者可藉由旋轉座體21,以改變第二偏光片41的偏振軸角度,使不同偏振角度的光通過待測樣品S,藉此可觀察待測樣品S中不同相之結構與型態。例如:在偏光模式下,可清楚觀察到澱粉結晶或昆蟲的複眼的微觀結構,故於偏光模式下可提升成像的解析度。 When the light source module 20 is turned on, if the polarization axes of the first polarizer 40 and the second polarizer 41 are perpendicular to each other, and the sample carrying module 10 carries the sample S to be measured without birefringence, the user The imaging of the sample S to be tested will not be seen. When the sample carrying module 10 carries the sample S to be measured having birefringence properties, the light emitted by the light emitting element 22 will sequentially pass through the second polarizer 41, the sample to be tested S, the lens module 30, and the first polarizer 40, and reach the image capture module 61, the operator can take an image of the sample S to be measured through the image capture module 61. In addition, since the first polarizer 40 is fixed on the image capturing module 61, the operator can rotate the base 21 to change the polarization axis angle of the second polarizer 41, so that light with different polarization angles can pass through the measurement. Sample S, by which the structure and shape of different phases in sample S can be observed. For example, in polarized light mode, the microstructure of starch crystals or compound eyes of insects can be clearly observed, so the resolution of imaging can be improved in polarized light mode.
圖6為依據本發明另一實施例的可攜式顯微鏡裝置組裝於影像擷取裝置的示意圖,圖7A至圖7D為圖6中可攜式顯微鏡裝置的剖面示意圖,請同時參閱圖6及圖7A所示。可攜式顯微鏡裝置7可與一影像擷 取模組61搭配使用,而影像擷取模組61係設置於一影像擷取裝置60上,例如影像擷取裝置60可以是智慧型手機、平板電腦、筆記型電腦、行車記錄器、相機、自拍器或穿戴式電子裝置。以下實施例的影像擷取裝置60將以智慧型手機為例進行說明。 6 is a schematic diagram of a portable microscope device assembled in an image capturing device according to another embodiment of the present invention, and FIGS. 7A to 7D are schematic cross-sectional views of the portable microscope device in FIG. 6. Please refer to FIG. 6 and FIG. 7A. Portable microscope device 7 can be captured with an image The capturing module 61 is used in combination, and the image capturing module 61 is disposed on an image capturing device 60. For example, the image capturing device 60 may be a smart phone, a tablet computer, a notebook computer, a driving recorder, a camera, Selfie or wearable electronics. The image capturing device 60 in the following embodiments will be described by taking a smart phone as an example.
可攜式顯微鏡裝置7可包括一連接元件75,設置於殼體71的一側,以將可攜式顯微鏡裝置7連接影像擷取模組61。本實施例中,連接元件75為一黏著層,且較佳的,黏著層可以為壓敏膠(pressure sensitive adhesive),即對壓力有敏感性的黏著劑,其可重複黏貼。因此,可攜式顯微鏡裝置7可藉由連接元件75直接貼附於影像擷取裝置60,安裝上極為便利。連接元件75亦可為一連接夾或以螺紋或卡合的機構連接,本發明不以此為限。 The portable microscope device 7 may include a connecting element 75 disposed on one side of the casing 71 to connect the portable microscope device 7 to the image capturing module 61. In this embodiment, the connecting element 75 is an adhesive layer, and preferably, the adhesive layer may be a pressure sensitive adhesive, that is, a pressure-sensitive adhesive, which can be repeatedly adhered. Therefore, the portable microscope device 7 can be directly attached to the image capturing device 60 through the connection element 75, which is extremely convenient for installation. The connecting element 75 may also be a connecting clip or connected by a thread or an engaging mechanism, which is not limited in the present invention.
可攜式顯微鏡裝置7包括一殼體71、一凸透鏡72以及一光源模組73。殼體11具有一樣品觀察面711及一出光孔712,出光孔712位於靠近樣品觀察面711的一側,樣品觀察面711位於殼體71相對於影像擷取模組61的一側。凸透鏡72設置於殼體71內部。本實施例中,凸透鏡72為一雙凸透鏡的非球面鏡。凸透鏡72具有一翼部721,且翼部721位於凸透鏡72之週緣。換言之,凸透鏡72的中央部分為雙面凸起的非球面鏡,而凸透鏡72的週緣部分則為扁平的翼部,由於翼部721係與成像無關的部分,故本發明並未限制翼部721的長度。 The portable microscope device 7 includes a housing 71, a convex lens 72, and a light source module 73. The housing 11 has a sample observation surface 711 and a light exit hole 712. The light exit hole 712 is located on a side close to the sample observation surface 711, and the sample observation surface 711 is located on a side of the housing 71 opposite to the image capturing module 61. The convex lens 72 is provided inside the case 71. In this embodiment, the convex lens 72 is an aspheric mirror of a biconvex lens. The convex lens 72 has a wing portion 721, and the wing portion 721 is located on the periphery of the convex lens 72. In other words, the central portion of the convex lens 72 is a double-sided convex aspherical mirror, and the peripheral portion of the convex lens 72 is a flat wing portion. Since the wing portion 721 is a portion not related to imaging, the present invention does not limit the length.
如圖7A所示,凸透鏡72的雙面凸起部分具有直徑d1。出光孔712具有一孔徑d2,孔徑d2與直徑d1的比值介於1至1.5之間,其可避免環境雜光進入凸透鏡72中,進而可避免環境雜光影響樣品成像的情形。 As shown in FIG. 7A, the double-sided convex portions of the convex lens 72 have a diameter d1. The light emitting hole 712 has an aperture d2, and the ratio of the aperture d2 to the diameter d1 is between 1 and 1.5, which can prevent ambient stray light from entering the convex lens 72, and thus can avoid the situation where the ambient stray light affects the imaging of the sample.
光源模組73包括一開關(未圖示)及一發光元件731,開關耦接於發光元件731。光源模組73設置於殼體71內,並位於影像擷取模組61與凸透鏡72之間,其中發光元件731的位置係對應於凸透鏡72的翼部721,故發光元件731係環繞凸透鏡72中央凸起的周圍設置。此外,發光元件731可以為發光二極體、雷射二極體或是螢光燈,其所發出的光線可以是可見光或非可見光。舉例來說,若發光元件731提供的是光是可見光 時,可作為觀察大部分樣品類型的光源,若發光元件731發出的光是紅外光或紫外光時,則可分別用於珠寶鑑定或用於驗鈔。 The light source module 73 includes a switch (not shown) and a light emitting element 731. The switch is coupled to the light emitting element 731. The light source module 73 is disposed in the housing 71 and is located between the image capturing module 61 and the convex lens 72. The position of the light emitting element 731 corresponds to the wing portion 721 of the convex lens 72. Therefore, the light emitting element 731 surrounds the center of the convex lens 72. Raised around. In addition, the light emitting element 731 may be a light emitting diode, a laser diode, or a fluorescent lamp, and the light emitted by the light emitting element 731 may be visible light or non-visible light. For example, if the light provided by the light-emitting element 731 is visible light It can be used as a light source for observing most types of samples. If the light emitted by the light-emitting element 731 is infrared light or ultraviolet light, it can be used for jewelry identification or banknote detection, respectively.
可攜式顯微鏡裝置7更可包括前述的樣品承載組件10,如圖7D所示,透光載體11係貼附於殼體71遠離影像擷取模組61一側的表面,此時,樣品觀察面711係位於透光載體11的外表面。於一般使用情況下,樣品觀察面711至凸透鏡72之表面的最短距離D係介於0.1mm至3mm之間,較佳的係介於0.3mm至2.0mm,更佳的,最短距離D可以介於0.5mm至1.2mm之間。當應用於高倍率或有景深需求時,最短距離D係介於0.1mm至10mm之間,較佳的係介於0.1mm至6.0mm。藉由前述的設置方式,可使發光元件731所發射出的光線由凸透鏡72的第一凸面722入射,並由凸透鏡72的第二凸面723出射,而聚焦至待測樣品S。 The portable microscope device 7 may further include the aforementioned sample carrying assembly 10. As shown in FIG. 7D, the transparent carrier 11 is attached to the surface of the casing 71 away from the image capturing module 61. At this time, the sample is observed The surface 711 is located on the outer surface of the transparent carrier 11. Under normal use, the shortest distance D between the sample observation surface 711 and the surface of the convex lens 72 is between 0.1mm and 3mm, and the best distance is between 0.3mm and 2.0mm. Between 0.5mm and 1.2mm. When applied to high magnification or depth of field requirements, the shortest distance D is between 0.1mm and 10mm, and the preferred distance is between 0.1mm and 6.0mm. With the foregoing arrangement, the light emitted by the light-emitting element 731 can be incident on the first convex surface 722 of the convex lens 72 and emitted from the second convex surface 723 of the convex lens 72 to be focused on the sample S to be measured.
需特別說明的是,上述實施例所指的樣品觀察面711可以為實體表面或是虛擬表面。以實體表面而言,如圖7B所示,當樣品承載組件10與殼體71相互接觸時,此時,樣品觀察面711實質上為殼體71相對於影像擷取模組61另一側的表面。另請參閱圖7C所示,以虛擬表面而言,當樣品承載組件10與殼體71之間存在些微距離時,此時,樣品觀察面711則為樣品承載組件10面對可攜式顯微鏡裝置7一側的表面。 It should be particularly noted that the sample observation surface 711 referred to in the above embodiment may be a solid surface or a virtual surface. In terms of a solid surface, as shown in FIG. 7B, when the sample carrying assembly 10 and the casing 71 are in contact with each other, at this time, the sample observation surface 711 is substantially the other side of the casing 71 relative to the other side of the image capturing module 61. surface. Please also refer to FIG. 7C. As far as the virtual surface is concerned, when there is a slight distance between the sample carrying assembly 10 and the housing 71, the sample observation surface 711 is the sample carrying assembly 10 facing the portable microscope device. 7 surface on one side.
如圖7D所示,殼體71更具有一開口713,開口713位於靠近影像擷取模組61的一側。因此,發光元件731所發出的光線係先由凸透鏡72的第一凸面722入射,並由第二凸面723出射後,再經出光孔712照射至樣品觀察面711,以聚焦於待測樣品S。接著,光線經待測樣品S反射後,再由凸透鏡72的第二凸面723入射,並由第一凸面722出射且經開口713後,入射至影像擷取模組61。而影像擷取模組61取得放大後的樣品影像後,使用者即可直接在影像擷取裝置60的顯示螢幕觀察樣品影像。 As shown in FIG. 7D, the casing 71 further has an opening 713, and the opening 713 is located on a side near the image capturing module 61. Therefore, the light emitted by the light-emitting element 731 is incident from the first convex surface 722 of the convex lens 72 and emitted from the second convex surface 723, and then is irradiated to the sample observation surface 711 through the light exit hole 712 to focus on the sample S to be measured. After the light is reflected by the sample S to be measured, it is incident again through the second convex surface 723 of the convex lens 72, exits from the first convex surface 722, and enters the image capturing module 61 after passing through the opening 713. After the image capture module 61 obtains the enlarged sample image, the user can directly observe the sample image on the display screen of the image capture device 60.
綜上所述,本發明是藉由樣品黏著件的凹部沾黏一待測樣品,並貼附於透光載體上,即完成顯微鏡樣品的製備,因此大幅簡化了傳統顯微鏡樣品的製備流程。此外,本發明的樣品黏著件採用沖壓凹部的設計方式,不僅可容置液態樣品或活體樣品,也有助於封閉待測樣品,並能防止液體外漏,故具有較佳的密封效果。再者,本發明的可攜式顯微鏡裝 置可輕易裝設於影像擷取裝置上,以拍攝微小物品的顯微結構,並可搭配一組偏光片,以觀察待測樣品內部不同相的微觀結構,故可達成提升成像解析度及方便攜帶的功效。 In summary, the present invention completes the preparation of a microscope sample by sticking a sample to be tested with the concave portion of the sample adhesive and attaching it to a light-transmissive carrier, thus greatly simplifying the preparation process of a traditional microscope sample. In addition, the sample adhesive part of the present invention adopts the design of the stamped recess, which can not only accommodate liquid samples or living samples, but also help to seal the sample to be tested, and can prevent liquid leakage, so it has a better sealing effect. Furthermore, the portable microscope device of the present invention It can be easily installed on the image capture device to capture the microstructure of small objects, and can be used with a set of polarizers to observe the microstructure of different phases inside the sample to be measured, so it can achieve improved imaging resolution and convenience Carrying effect.
上述實施例並非用以限定本發明,任何熟悉此技藝者,在未脫離本發明之精神與範疇內,而對其進行之等效修改或變更,均應包含於後附之申請專利範圍中。 The above embodiments are not intended to limit the present invention. Any person familiar with the art should make equivalent modifications or changes to the present invention without departing from the spirit and scope of the present invention, which should be included in the scope of the attached patent application.
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TW106102213A TWI664416B (en) | 2016-02-05 | 2017-01-20 | Reflecting microscope module and reflecting microscope device |
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JP2017160419A (en) | 2017-09-14 |
CN107045188B (en) | 2020-03-17 |
CN107045190A (en) | 2017-08-15 |
JP6585642B2 (en) | 2019-10-02 |
JP2017138598A (en) | 2017-08-10 |
CN107045188A (en) | 2017-08-15 |
JP2017138603A (en) | 2017-08-10 |
TWI664416B (en) | 2019-07-01 |
CN107045191B (en) | 2020-06-16 |
TW201802449A (en) | 2018-01-16 |
CN107045191A (en) | 2017-08-15 |
TW201728956A (en) | 2017-08-16 |
JP6521331B2 (en) | 2019-05-29 |
AU2017200620A1 (en) | 2017-11-23 |
JP6745734B2 (en) | 2020-09-02 |
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