TWI422433B - A low-temperature biomedical homogenizes device - Google Patents

Description

Biomedical homogenization device

The present invention relates to a biomedical homogenization device, and more particularly to a biomedical homogenization device having an improved homogenization effect while having a low temperature.

In the field of molecular biology research, it is often necessary to extract DNA, RNA or protein of various species to be tested or identified in order to detect and observe subsequent specific factors. In general, various tissues (eg, skin, liver, kidney, etc.), cells, vaccines, etc., may be doped with other impurities during initial treatment, or even because the volume of the tissue is too large to be directly utilized. It is processed in a homogeneous manner, making it suitable for subsequent screening or purification procedures.

The traditional homogenization method can adopt high-speed centrifugation, ultrasonic vibration or machine cutting. In the case of tissue homogenization, the tissue fragments to be homogenized are usually placed in a micro tube, and then cut by a tool with a blade at the head to inhale the tissue fragments in the micro tube under high speed operation. The blade portion of the machine performs shearing, dispersing, and the like in the space gap, and generates a high-pressure squirt from the side of the head of the machine to quickly achieve the effect of particle fragmentation of the tissue. However, the cutting method of the conventional implement is easy to cause a large amount of residual tissue debris in the head of the implement, and the effect of homogenizing the tissue debris is incomplete; and, more likely, the cleaning of the head of the implement is inadvertent. In the repeated use of the machine, problems such as tissue debris pollution and bacteria are generated, which seriously affects the experimental quality of the subsequent operation of the tissue fragments.

Therefore, it is done through multi-speed centrifugal method of homogenizing substances jobs tissues, cells, etc., and the market has also developed the high-speed centrifugal homogenizer, such as Chi Tak International Limited of trafficking Bullet Blender ^TM homogenizer. Taking tissue homogenization as an example, it is placed in a microtube filled with a tissue to be homogenized, and at least one magnetic bead is placed, and the microtube is placed in a slot provided by the homogenizer, and The high-speed centrifugation of the homogenizer enables the magnetic beads to repeatedly collide with the tissue to be homogenized in the microtube to achieve the purpose of tissue fragmentation and homogenization.

However, the high-speed centrifugal homogenizer described above can only drive the slots in which the microtubes are placed to rotate synchronously, thereby producing the effect of high-speed centrifugation. Thus, the frequency at which the magnetic beads repeatedly collide in the microtube is limited by the centrifugal speed of the homogenizer, and the repeated impact frequency of the magnetic beads in the microtube cannot be increased at a fixed rotational speed, resulting in tissue homogenization. The effect is obviously limited; even in the case of synchronous high-speed centrifugal rotation, the magnetic beads are easy to fall on the bottom of the microtube, and only frequent micro-shocks can be generated at the bottom of the microtube, and the comprehensiveness is always impossible. Impacting the tissue to be homogenized in the microtube seriously affects the effect of homogenization of the tissue, and the experimental quality of subsequent screening, purification or identification of the homogeneous tissue.

In view of this, it is indeed necessary to develop a biomedical homogenizing device capable of improving the homogenization effect, so as to solve various problems as described above by simultaneous and independent high-speed centrifugation of a box containing microtubes.

SUMMARY OF THE INVENTION The main object of the present invention is to improve the above disadvantages to provide a biomedical homogenization apparatus capable of increasing the frequency of repeated impact of magnetic beads in a microtube at a fixed rotational speed to enhance the effect of homogenization of the substance.

A second object of the present invention is to provide a biomedical homogenization device capable of comprehensively impacting a substance to be homogenized in a microtube, to have the effect of complete homogenization of the substance and to maintain the quality of subsequent experiments.

Still another object of the present invention is to provide a biomedical homogenization apparatus capable of performing homogenization work in a low temperature environment to improve homogenization effect and device usability.

In order to achieve the foregoing object, the biomedical homogenizing apparatus of the present invention comprises: a body provided with at least one power wheel disposed on an inner wall of the base; and a rotating assembly rotatably assembled The rotating body includes a driving shaft and a two-spindle, the driving shaft is coupled to the power wheel, and the two ends of the driving shaft are respectively provided with the two rotating wheels, and the driving shaft drives the rotating table to rotate, and At least one of the rotating wheels is provided with at least one driven wheel, and the power wheel drives the driven wheel to rotate relative to the turntable; and at least one receiving member is rotatably disposed between the two rotating wheels and is coupled with the driven wheel, The driven wheel drives the accommodating member to rotate relative to the turntable.

The biomedical homogenization device of the present invention may be selected to have two sets of power wheels provided in the seat body, the two sets of power wheels are respectively disposed on corresponding inner walls of the seat body, and each set of power trains is composed of two identical teeth and a modulus. The power wheels are stacked together. Moreover, each of the two turntables is provided with a set of driven wheels, and each set of driven wheels is composed of two driven wheels of the same number of teeth and a modulus, and each set of driven wheels of the two turntables are arranged offset from each other.

The two ends of the driving shaft can respectively penetrate the two sets of power wheels, and a power source is disposed at one end of the driving shaft.

Wherein, the two carousels can be respectively engaged with the respective ones of the two ends of the driving shaft, and are fixed by a buckle.

Furthermore, the driven wheel train may have a rotating portion and a set of connecting portions, the rotating portion may be rotated relative to the turntable, and the connecting portion may be disposed on an inner surface of the turntable, and the connecting portion may be provided for the capacity The component is assembled to limit the receiving member between the two turntables.

Wherein, the two turntables are not provided with the driven wheel, and another array connector is arranged, and the array connector can be rotated relative to the turntable.

One end of the accommodating member may be assembled to the assembly portion of the driven wheel, and the other end of the accommodating member is assembled to the assembly of the turntable.

The accommodating member can be divided into a plurality of slots, and the plurality of slots are closed by a cover, and the cover is pivotally disposed at a periphery of the accommodating member.

In addition, the biomedical homogenizing device of the present invention may further comprise a magnetic member on the outer peripheral wall of the driving shaft, the magnetic member covering the outer peripheral wall of the driving shaft for providing a magnetic force.

In addition, in the biomedical homogenization device of the present invention, a gas circulation component may be further disposed in the body to control the temperature of the air inside the body, preferably in a homogeneous environment of 4 °C.

The above and other objects, features, and advantages of the present invention will become more apparent from the aspects of the appended claims. The biomedical homogenizing device of the present invention comprises a body 1, a rotating component 2 and at least one receiving member 3, the rotating component 2 is rotatably assembled to the base body 1, and the rotating component 2 is used for driving The rotation of the accommodating member 3. Thereby, the micro tube 4 (as shown in FIG. 3) can be placed in the accommodating member 3, so that when the accommodating member 3 is synchronously rotated and independently rotated with the rotating unit 2, each of the accommodating members 3 is increased. The magnetic beads 41 in the microtube 4 impact frequency, and the effect of comprehensively reducing and homogenizing the material is achieved.

The configuration of the accommodating member 3 determines the design of the biomedical homogenizing device of the present invention, so that each of the accommodating members 3 can generate synchronous rotation and independent rotation, and increase the number of each of the micro tubes 4 The frequency of impact of the magnetic beads 41 is the main principle. The accommodating member 3 can be at least one set, but the number of the accommodating members 3 is not limited. In order to achieve the above-mentioned comprehensive shredding and homogenization, a large number of micro tubes can be provided at the same time. 4, the present invention is selected to be provided in a symmetrical configuration with four accommodating members 3, and corresponding to the four accommodating members 3 as described below as a preferred embodiment of the present invention, please refer to the first to fifth The figure shows the details below.

Referring to FIG. 1 , which is a preferred embodiment of the present invention, the biomedical homogenizing device comprises a body 1, a rotating component 2 and four receiving members 3, and the rotating component 2 is rotatably assembled. The housing 1 and the rotating assembly 2 are used to drive the rotation of each of the receiving members 3.

The base 1 is only used for supporting or accommodating the rotating component 2, so that the aspect of the base 1 should not be limited. In particular, as shown in FIG. 2, the seat body 1 of the present embodiment can have an accommodating space S for assembling the rotating component 2 in the accommodating space S. Moreover, an object window W can be opened in the body 1, and the object window W is preferably adhered by an opening and closing cover B. Even more selectively, other devices (for example, gas circulation components, etc.) are additionally assembled to the seat body 1 for the biomedical body of the present invention. The homogenization device can be used more widely or in a low temperature environment.

Referring to Figures 1 and 2, the base 1 is provided with at least one power wheel 11 which can be driven by a power source. In this embodiment, the power source M can be a motor. At least one power wheel 11 is formed in two groups and respectively disposed on corresponding inner walls of the seat body 1. The at least one power wheel 11 can be composed of two power wheels, and is preferably superposed on each other by two gears having the same number of teeth and the modulus, so that when the rotating component 2 drives the accommodating members 3 to rotate, Each of the accommodating members 3 is subjected to a homogenization operation at the same centrifugal speed.

The rotating assembly 2 is rotatably assembled to the base 1 and is preferably coupled between the two sets of power wheels 11. The rotating component 2 of the embodiment includes a driving shaft 21 and two rotating wheels 22, and the driving shaft 21 is coupled with the two sets of power wheels 11, in particular, the two ends of the driving shaft 21 respectively penetrate the two sets of power The wheel 11 is provided with a power source M at one end of the driving shaft 21 to output power by the power source M to drive the rotation of the rotating component 2. Moreover, the two ends of the driving shaft 21 are respectively provided with the two rotating discs 22, and the joint portion of the two rotating discs 22 and the driving shaft 21 can form a non-circular shape, so that the two can rotate synchronously. In this embodiment, A latching portion 211 is disposed on each of the two ends of the driving shaft 21 for socketing the two rotating discs 22, and is fixed by a buckle 212 to ensure that the two rotating discs 22 can rotate synchronously with the driving shaft 21.

In addition, the present invention can also be provided with a magnetic member 213 disposed on the outer peripheral wall of the driving shaft 21. The magnetic member 213 is preferably wrapped around the outer peripheral wall of the driving shaft 21, as shown in the first drawing. Provide magnetic force to attract the micro Magnetic beads 41 in the tube 4. The arrangement of the magnetic member 213 is selected but not limited to the complete wrapping of the driving shaft 21, which can be easily understood by those skilled in the art and will not be described in detail herein.

Referring to FIG. 1 again, the present invention is optional, but not limited to, one of the rotating disks 2 of the rotating assembly 2 is provided with at least one driven wheel 23 for engaging the receiving member by the driven wheel 23. 3. The driven wheel 23 can be driven by the power wheel 11 and rotated relative to the turntable 22. The driving mode can be any conventional driving manner, for example, by using a gear to mesh between the two for indirect transmission; or in the outer circumference of the turntable 22 A ring inner tooth is provided, and the driven wheel 23 meshes with the inner tooth; or as shown in the embodiment, the driven wheel 23 and the power wheel 11 are rotated by a link 24 such as a chain or a belt. In the embodiment, the two turntables 22 are preferably provided with a set of driven wheels 23 (ie, two sets of identical numbers of teeth and two driven wheels of the module, which are briefly described as the driven wheel 23). The driven wheels 23 of the two turntables 22 are preferably arranged in a mutually dislocated manner, as shown in the figure of FIG. 1 , so that the driven wheels 23 of the two turntables 22 are in a cross-shaped setting manner for each of them. When the accommodating member 3 is assembled, a proper distance between each of the accommodating members 3 can be left to avoid affecting the turning effect during centrifugation. Moreover, when the second turntable 22 is not provided with the driven wheel 23, an array connector 221 is further provided, and the set of parts can be rotated relative to the turntable 22, and the set of connecting pieces 221 and the driven wheel 23 correspond to each other. For receiving one end of the accommodating member 3.

Each of the driven wheels 23 of the driven wheel 23 has a rotating portion 231 and a set of connecting portions 232. The rotating portion 231 is rotatable relative to the turntable 22, and the connecting portion 232 is preferably protruded. The inner surface of the turntable 22 is assembled by the other end of the receiving member 3 by the set portion 232, The accommodating member 3 is disposed between the two turrets 22, and the rotating portions 231 of the driven wheels 23 correspond to the power wheels 11 of the base body 1 to be engaged with each other by a linking member 24.

In order to cope with the configuration of the plurality of sets of the power wheel 11 and the plurality of sets of the driven wheels 23 of the present embodiment, the rotating component 2 of the biomedical homogenizing device of the present invention is selected to be provided with a plurality of linking members 24, and the plurality of linking members 24 are respectively connected around the power wheel. 11 and the driven wheel 23, the power wheel 11 and the driven wheel 23 are rotated by the surrounding motion of the linking member 24, in particular, because the number of teeth and the modulus of the power wheel 11 are larger than the number of teeth and the modulus of the driven wheel 23, The driven wheel 23 is forced to make more revolutions.

With reference to the first and third figures, each of the accommodating members 3 is rotatably disposed between the two turrets 22, and preferably has a set of holes on the corresponding side walls of each of the accommodating members 3. 31, the assembly holes 31 at one end of each of the accommodating members 3 are assembled to the assembly portion 232 of the driven wheel 23, and the assembly holes 31 at the other end of each of the accommodating members 3 are disposed on the turntable 22 The assembly member 221 is configured to make the rotation of the accommodating member 3 more balanced and stable. The shape and size design of the accommodating member 3 is mainly based on the fact that the micro-tubes 4 can be mounted and pivoted between the two turrets 22 of the rotating assembly 2, so that the accommodating member is not limited thereto. 3 should be presented.

In particular, as shown in FIG. 3, the accommodating member 3 is formed in a box shape, and the box-like accommodating member 3 is divided into a plurality of cavities 32, and the diameter of each of the accommodating grooves 32 is matched with the The diameter of the microtube 4 is designed so that the microtube 4 can be accommodated in the receptacle 32, and it is a better principle not to cause excessive left and right sloshing in the receptacle 32, which is suitable for those skilled in the art. Easy to understand, no more details. Moreover, the number of the receptacles 32 of the receiving member 3 is one The cover plate 33 is closed, and the cover plate 33 is preferably pivoted on the periphery of the accommodating member 3 to facilitate opening and closing, in particular, it can achieve better fastening effect when closed, and does not occur at high speed centrifugation. The cover plate 33 is loosened to cause the microtube 4 to fall.

It should be noted that the biomedical homogenizing device of the present invention can bear the above, and a gas circulation component 5 (shown in FIG. 2) can be further disposed in the body 1 to be controlled by the gas circulation component 5. The temperature of the air in the body 1 enables the biomedical homogenizing device of the present invention to provide a different homogenization environment, preferably maintained at a low temperature of 4 ° C, and is widely applicable to various animal tissues, cells or strains. Moreover, a tuning control 6 can be disposed on the outer wall of the body 1 to control the menu of the biomedical homogenizing device of the present invention in the homogenization process, and various animal tissues, cells or The ambient temperature suitable for the species to achieve better material homogenization.

Referring to Figures 4 and 5, it is only one aspect of the biomedical homogenizing device of the present invention that is configured with the body 1, the rotating assembly 2 and the plurality of receiving members 3, thereby illustrating the homogenization process of the present invention. The situation of action.

Before operating the biomedical homogenization device of the present invention, the substance to be homogenized (for example, animal tissue, cells or strains, etc.) is first added to the microtube 4, and at least one of the microtubes 4 is simultaneously embedded. The magnetic beads 41 (as shown in FIG. 3) are placed in the receptacles 31 of the accommodating members 3, and the appropriate rotation speed and temperature are set through the adjustment control 6 respectively. To perform homogenization at the same time. In this way, the power source M outputs power to drive the rotation of the driving shaft 21 of the rotating assembly 2, so that the number of the accommodating members 3 disposed between the two rotating disks 22 of the rotating assembly 2 can be the same. At the same time, the driven wheel 23 disposed on the two turntables 22 can be rotated by the power receiving member 11 and the linking member 24, so that the plurality of receiving members 3 can rotate relative to each other, so that the slaves 22 are coupled to the slave. The accommodating member 3 of the moving wheel 23 can not only rotate synchronously with the rotating assembly 2, but also the accommodating member 3 can be independently rotated by the driven wheel 23 to increase the accommodating member 3 at a stable rotational speed. The frequency of the flipping is such that the magnetic bead 41 can repeatedly collide within the microtube 4 to achieve the effect of complete homogenization.

In summary, the main feature of the biomedical homogenizing device of the present invention is that the rotating component 2 can easily drive the accommodating member 3 to be synchronized and independent by the common connection configuration of the power wheel 11 and the driven wheel 23. Rotating to increase the inversion frequency of the accommodating member 3 at a steady rotation speed, thereby increasing the number of repeated collisions of the magnetic beads 41 in the microtube 4, and comprehensively impacting the microtube 4 to be homogeneous The substance is turned into a substance that enhances the homogenization of the substance. Even when the biomedical homogenizing device of the present invention operates at a medium-high speed, the mixing frequency of the accommodating member 3 can be increased to maintain a better homogenization effect; and it is not caused by long-time high-speed rotation. If the conventional magnetic bead 41 falls on the bottom of the microtube 4, the biomedical homogenizing device of the present invention can be maintained at a stable and homogenized effect without being limited to the speed of the centrifugal rotation.

The biomedical homogenization device of the invention can increase the repetitive impact frequency of the magnetic beads in the micro tube at a fixed rotation speed, so as to achieve the effect of improving the homogenization of the substance.

The biomedical homogenization device of the invention can comprehensively impact the substance to be homogenized in the micro tube, and has the effect of complete homogenization of the substance, thereby achieving the effect of maintaining the better quality of the subsequent experiment.

The biomedical homogenization device of the invention can perform homogenization operation in a low temperature environment to improve the homogenization effect, and is widely applicable to various animal tissues, cells or strains, and relatively achieves the effect of improving the usability of the device.

While the invention has been described in connection with the preferred embodiments described above, it is not intended to limit the scope of the invention. The technical scope of the invention is protected, and therefore the scope of the invention is defined by the scope of the appended claims.

〔this invention〕

1‧‧‧ body

11‧‧‧Power Wheel

2‧‧‧Rotating components

21‧‧‧Active shaft

211‧‧‧ card joint

212‧‧‧ buckle

213‧‧‧Magnetic parts

22‧‧‧ Turntable

221‧‧‧Components

23‧‧‧ driven wheel

231‧‧‧Rotating Department

232‧‧‧Team

24‧‧‧ linkages

3‧‧‧ accommodating components

31‧‧‧Group holes

32‧‧‧ 容容

33‧‧‧ cover

4‧‧‧Microtubules

41‧‧‧Magnetic beads

5‧‧‧ gas cycle components

6‧‧ ‧ control

S‧‧‧ accommodating space

W‧‧‧Drawing window

B‧‧‧Open and close cover

M‧‧‧ power source

Figure 1 is a perspective exploded view of the biomedical homogenization device of the present invention.

Fig. 2 is a perspective view showing the combined configuration of the biomedical homogenizing device of the present invention.

Figure 3: Schematic diagram of some of the components of the biomedical homogenization device of the present invention.

Figure 4 is a schematic cross-sectional view showing the combined configuration of the biomedical homogenizer of the present invention.

Figure 5: Schematic diagram of the operation of the biomedical homogenization device of the present invention.

1. . . Seat

11. . . Power wheel

2. . . Rotating component

twenty one. . . Drive shaft

211. . . Card joint

212. . . Buckle

213. . . Magnetic parts

twenty two. . . Turntable

221. . . Group connector

twenty three. . . driven wheel

231. . . Rotating part

232. . . Group

twenty four. . . Linkage

3. . . Storing member

31. . . Group hole

M. . . Power source

Claims (11)

A biomedical homogenization device comprising: a body having at least one power wheel disposed on an inner wall of the base; a rotating assembly rotatably assembled to the base, the rotating component The driving shaft is coupled to the power wheel, and the two ends of the driving shaft are respectively disposed with the two rotating wheels, the driving shaft drives the rotating wheel, and at least one of the rotating wheels is provided with at least one a driving wheel, the power wheel driving the driven wheel to rotate relative to the turntable; and at least one receiving member rotatably disposed between the two rotating wheels, and being coupled to the driven wheel, the driven wheel driving the receiving member Rotate relative to the turntable. The biomedical homogenizing device according to claim 1, wherein the seat body is provided with two sets of power wheels, and the two sets of power wheels are respectively disposed on corresponding inner walls of the seat body, and each set of power trains It is formed by two power wheels of the same number of teeth and a module. The biomedical homogenizing device according to claim 2, wherein the two rotating wheels are provided with a set of driven wheels, and each group of driven wheels is composed of two driven wheels of the same number of teeth and a module, and the driven wheel The sets of driven wheels of the two turntables are arranged offset from each other. The biomedical homogenizing device according to the second or third aspect of the invention, wherein the two ends of the driving shaft respectively penetrate the two sets of power wheels, and a power source is disposed at one end of the driving shaft. Biomedical homogeneous packaging as described in items 1, 2 or 3 of the scope of application The driven wheel train has a rotating portion and a set of connecting portions, the rotating portion is rotated relative to the rotating plate, and the connecting portion is disposed on an inner surface of the rotating plate to assemble the receiving member by the connecting portion. And limiting the receiving member between the two turntables. The biomedical homogenizing device according to the first, second or third aspect of the patent application, wherein the two rotating wheels are not provided with the array wheel, and the group of connecting members is rotatable relative to the rotating plate. The biomedical homogenizing device according to claim 6, wherein one end of the receiving member is assembled to the connecting portion of the driven wheel, and the other end of the receiving member is assembled to the turntable. Group connector. The biomedical homogenizing device according to claim 1, wherein the outer peripheral wall of the driving shaft is provided with a magnetic member, and the magnetic member is coated on the outer peripheral wall of the driving shaft for providing a magnetic force. The biomedical homogenizing device according to claim 1, 2 or 3, wherein a gas circulation component is further disposed in the body for controlling the temperature of the air inside the body. The biomedical homogenizing device according to the first, second or third aspect of the patent application, wherein the two carousels are respectively engaged with each of the two engaging portions of the two ends of the driving shaft, and are fixed by a buckle. The biomedical homogenizing device according to the first, second or third aspect of the patent application, wherein the receiving member has a plurality of pockets, the plurality of slots are closed by a cover plate, and the cover is pivoted It is disposed on the periphery of the accommodating member.