1283298 九、發明說明: 【發明所屬之技術領域】 本發明係有關於一種涉及電子物理學、病毒學、細胞 學、病理學技術領域。 【先前技術】 由於SARS病毒是一個人類從未認知的薪新的病毒,來 源於自然界,是一種結構非常複雜的新型病原體,感染後 發病急且重,危險程度大。SARS病毒主要是通過呼吸道傳 播,給預防、控造成很大的困難。除紫外綫、化學藥物消 毒外,目前尚其他無有效抑制其傳播的理想方法。 活性氧是以氧原子0形式存在的氧,它具有極强的氧 化能力,可以迅速與各種病毒、細菌以及空氣污染物發生 氧化反應幷達到清除目的。由於活性氧具有非常活波的特 性,因此在空氣中的存在時間非常短,它們_方面可以與 空氣中的病毒、細g和污_發生反應,同時如果沒有適 當的反應㈣,則可以與氧氣反應而產生臭氧〇” 活!·生氧I負離子和臭氧都可以通過高麈靜電激發和 利用可㈣控制電容的充放電來獲得高頻電壓的方式產 生,但是隨著電子似構造和條件的不同,其產生活性氧、 臭氧的濃度相㈣倾妹Μ錢,當衫同濃度段時 的活性乳、臭氧作用於病毒、病原體時,作用結果也完全 不同。特別疋在活性氧、純的不同濃度段時對邏病毒 的作用結果也完全不同。 1283298 【發明内容】 有鑑於SARS病毒主要是通過呼吸道傳播,給預防、控 造成很大的困難,本發明人有感其事態嚴重,遂竭盡心智, .. 悉心研究克服,憑從事該項產業多年之經驗累積,進而研 發出一種有效、準確的活性氧、臭氧濃度電子設備,以對 SARS病毒進行殺死。 為達上述之目的,本發明是這樣實現的:一種在溫度 爲15°C-32°C,大氣壓力爲748mmHgh-765mmHgh,相對濕度 爲20%-85%時,電子設備所發生活性氧、臭氧的濃度爲〇·丨 鲁 -97mg/m3濃度段時,活性氧、臭氧作用時間爲1分鐘以上, 可有效的殺死SARS病毒。 在上述條件下,活性氧、臭氧最佳濃度段爲 mg/m3,作用時間爲1分鐘至15分鐘時可殺死SARS病毒。 本發明技術的優點在於有效的提供一種準確的活十生 氧、臭氧濃度段,與傳統的消毒方法相比,可準確、有效 的殺死SARS病毒。 【實施方式】 為使貴審查委員瞭解本發明之目的、特徵及功致,1283298 IX. DESCRIPTION OF THE INVENTION: TECHNICAL FIELD OF THE INVENTION The present invention relates to the field of electronic physics, virology, cytology, and pathology. [Prior Art] Because the SARS virus is a newly recruited virus that humans have never recognized, it originates from nature and is a new type of pathogen with very complicated structure. The infection is acute and serious, and the degree of danger is high. The SARS virus is mainly transmitted through the respiratory tract, which causes great difficulties in prevention and control. In addition to UV and chemical drugs, there are currently no other ideal methods to effectively inhibit their spread. Active oxygen is oxygen in the form of oxygen atom 0. It has a strong oxidizing ability and can be rapidly oxidized with various viruses, bacteria and air pollutants to achieve the purpose of removal. Since active oxygen has very active wave characteristics, it exists in air for a very short time. They can react with viruses, fine g and dirt in the air, and can react with oxygen if there is no proper reaction (4). Reaction produces ozone 〇" Live! · Oxygen I negative ions and ozone can be generated by high-electrostatic electrostatic excitation and by using (4) controlling the charge and discharge of the capacitor to obtain high-frequency voltage, but with the electron-like structure and conditions The concentration of active oxygen and ozone is generated. (4) When the active milk in the same concentration range and the ozone act on the virus and the pathogen, the effect is completely different. Especially in the different concentrations of active oxygen and pure The effect of the segmentation on the virus is also completely different. 1283298 [Summary of the Invention] In view of the fact that the SARS virus is mainly transmitted through the respiratory tract, it causes great difficulties for prevention and control, and the inventor feels that the situation is serious and he is doing his best. .. Carefully research and overcome, with the accumulation of experience in the industry for many years, and then develop an effective and accurate active oxygen, ozone The electronic device is used to kill the SARS virus. For the above purposes, the present invention is achieved by a method at a temperature of 15 ° C to 32 ° C, an atmospheric pressure of 748 mm Hgh-765 mmHgh, and a relative humidity of 20% - At 85%, when the concentration of active oxygen and ozone generated in the electronic device is 〇·丨鲁-97mg/m3, the active oxygen and ozone action time is more than 1 minute, which can effectively kill the SARS virus. Under the above conditions The optimal concentration range of active oxygen and ozone is mg/m3, and the SARS virus can be killed when the action time is from 1 minute to 15 minutes. The advantage of the present technology is that it effectively provides an accurate live oxygen and ozone concentration range. Compared with the traditional disinfection method, the SARS virus can be killed accurately and effectively. [Embodiment] In order to make the reviewer understand the purpose, characteristics and merits of the present invention,
茲藉由下述具體之實施例,並配合所附之圖式,對本發明 做一詳細說明,說明如后: X 請參閱第一、二圖,在室内溫度爲3〇°c,大氣壓力爲 757mmHgh,相對濕度爲52%時,電子設備所發生活性氧、臭 氣的濃度爲0.1-97mg/m3時,活性氧、臭氧作用1〇分鐘= 6 1283298 上時間,對SARS病毒的殺死率爲99. 68%。 活性氧、臭氧對SARS病毒殺死率的實施例: 將SARS病毒特製檢測箱,放入P3實驗室生物安全櫃 内,采用SZ-800B活性氧、臭氧發生機,把機器置於生物 安全櫃内,采用24孔培養板,在培養板的每個孔内放入 0. lml SARS病毒原液,蓋上檢測箱密閉蓋。檢測箱下端平 衡壓力孔的乳膠管放於盛有次氯酸鈉液體的容器内。 打開SZ-800B機電源開關産生活性氧、臭氧,氣體通 過乳膠管進入檢測箱,通氣30分鐘,打開檢測箱,取出 24孔培養板,置於生物安全櫃内,將孔中病毒稀釋成ΙίΓ1 、1(Γ2、1(Γ3、1(Γ4、1(Γ5、10_6 的不同濃度,接種 100# 1 於96孔培養板的Vero Ε6細胞孔内,每個病毒濃度設4 個孔,幷設不經活性氧、臭氧處理的病毒及正常細胞對照 〇 將96孔培養板於37°C下培養,72小時後觀察結果,記 錄出現病變孔數,計算50%病變劑量,與對照組比較,計算 殺死率,見第二圖。 本發明顯係利用自然法則之技術思想之高度發明,較 傳統復健療法更為簡便,亦有實質功效增進與產業上的高 度利用性,完全合於發明專利之法定要件,爰依法提出發 明專利申請。 【圖式簡單說明】 1283298 第一圖,爲本發明的臭氧濃度與時間的作用關係。 第二圖,爲活性氧、臭氧在0. l-70mg/m3濃度段時對SARS 病毒的殺死率。 【主要元件符號說明】The present invention will be described in detail by the following specific embodiments, with reference to the accompanying drawings, which are illustrated as follows: X Please refer to the first and second figures, the indoor temperature is 3 〇 ° C, and the atmospheric pressure is 757mmHgh, when the relative humidity is 52%, when the concentration of active oxygen and odor generated by the electronic device is 0.1-97mg/m3, the active oxygen and ozone act for 1〇 minutes = 6 1283298. The kill rate of SARS virus is 99. 68%. Examples of the killing rate of reactive oxygen and ozone on SARS virus: The SARS virus special detection box is placed in the P3 laboratory biosafety cabinet, and the machine is placed in the biological safety cabinet by using SZ-800B active oxygen and ozone generator. , using a 24-well culture plate, put 0. lml SARS virus stock solution in each well of the culture plate, and cover the test box with a closed lid. The latex tube that balances the pressure hole at the lower end of the test chamber is placed in a container containing sodium hypochlorite liquid. Turn on the SZ-800B machine power switch to generate active oxygen and ozone. The gas enters the test box through the latex tube, ventilate for 30 minutes, open the test box, take out the 24-well culture plate, place it in the biological safety cabinet, and dilute the virus in the hole into ΙίΓ1 , 1 (Γ2, 1 (Γ3, 1 (Γ4, 1 (Γ5, 10_6 different concentrations, inoculated 100# 1 in the 96-well culture plate of Vero Ε6 cell wells, each virus concentration set 4 holes, set no The 96-well culture plate was cultured at 37 °C with active oxygen, ozone-treated virus and normal cell control. The results were observed after 72 hours. The number of lesions was recorded and the 50% lesion dose was calculated. Compared with the control group, the kill was calculated. The mortality rate is shown in the second figure. The invention is based on the high-invention of the technical idea of the natural law, which is simpler than the traditional rehabilitation therapy, and has the substantial effect enhancement and high industrial utilization, and is fully compatible with the invention patent. The statutory requirements, 提出 提出 70 70 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 283 283 283 283 283 283 283 283 283 283 283 283 283 283 283 283 283 283 283 283 283 283 臭氧 臭氧 臭氧 臭氧 臭氧 臭氧 臭氧 臭氧 臭氧Concentration section The kill rate of the SARS virus. [Main component symbol description]