496110 經濟部中央標準局員工消費合作社印製 A7 ____________B7五、發明説明（丨） 【發明之應用範疇】 本發明係關於一種電化學感測器，特別是關於一種利用印刷電路板 之材料與製程製得，可作為電化學感測器之電極元件。 【發明之背景】 按印刷電路板(Printed Wiring Board or Printed Circuit Board; PWB or PCB)是依電路設計，將連接電路零件之電氣佈線繪製成佈線圖 形，再以特定的加工方法，在絕緣基板上使電氣導體重現所構成的電路 板。其主要作用是將各項電子零件藉由形成在電路板的電子線路連接起 來，以達到中繼傳輸，發揮各項電子零組件功能之目的。 蓽用的印刷電路板製程有加成法(additive process)及減成法 (subtractive process) 〇加成法是指在絕緣的電路基板表面，以化 學反應的方法，錢產生所f導電電路的餘。減成法是指將具有導電 表層的電路基板，以化學反麟方法，將表面局部崩的導體去除，而 作成電路板的製程。 如美國專利第5437’號（ElectrGdiemicai Sensor)說明書指出， 鮮印刷電路板*適合作為電化學分析電極之用，乃是習知的觀念。這 是由於印刷電路㈣導電表層的㈣，—般需要具有較高反應性的材 料，如鋼、鎳等導電金屬，才能符合加工容易、大量生產，簡化製程、 與價格低廉的要求。但這些本身具高反應性的材料，在保存時很容易和 空氣或其他物質反應，致使表面性質不易控制；在使用時，也很容易盘 反應物產生轉，而造成干擾，所以無法作為魏學分細電择。” 為改善上述表面性質穩定性的問題，印刷電路板利用非導電性材 料，如：絕緣綠漆，或反應性很低的導電貴金屬材料，如：金、白金、 3 210X 297 公釐） (請先閲讀背面之注意事項再填寫本頁) .· 訂 it泉 496110 A7 -—--- 五、發明説明（Λ ) ----- =二ΓΓ導電金屬電路包覆，以防止導電金屬底材與空氣或其 的問製程’確實有效的解決了印刷電路板表面性質穩定性 但由下面㈣日回了印刷電路板的品質’同時也可以保持長時間的穩定。 面之注意事項再填寫本頁) 準印刷•路板7可知道’就算表層已鍍上穩定高的導電金騎料，標 电產品，运是沒有魏作魏化學分极甩雜的要求。 印刷電路板的外層導電層製程有：化學浸錢法及電鍵法7 雜笠利用紐銅、錄的高反應性，與貴金屬如金、白金、 冋穩讀的㈣差異’將製成之銅、.鎳電路板置於貴金屬離子電解 之〜=學麵反應將t金屬沉積於底材上，如此可崎底材以貴金 =王覆1。由於此方法只能在底材战_騎金屬，紐健底材完 物隔離，在電化學電極的應用上’量測時仍易受干擾，所以尚 無法作為電化學分析的電極。 電錢法：是一般習用的印刷電路板電路的外層製程，可以得到較厚 =金層’但是在去除光阻劑與底材時，線路邊緣的縱切面會露出底材。 =為電化學反應電極時，底材會與被測物反應，在電化學電_應用上， 量測時會受干擾，卿也無法作為電化學分析的電極。 、為達到電化學電極應用的目的，美國專利54379㈣揭示一種以物 理/儿積法’於絕緣基材上製作貴金屬薄膜，再將之黏貼在—硬或軟的基 材上，而得到適合於電化學應用之工作電極，至於對極或參考極則採用 了標準的印刷電路板。此方法從理論上可以解決上述的問題，但其製程 複雜、加上以物理沉積法製作貴金屬薄膜的成本高，與應用印刷電路二 作為電極，以降低成本的本意不合。 【發明之目的】 本紙張尺國國家標準（CNs y A4規格（ A7 B7 五、發明説明（3 ) 本發月之目的在於提供—種新穎的製程，可在標準的印刷電路板電 路外層鍍上所#厚度貝金屬材料，以使印刷電路板達到作為電化學電極 及感測器之規格要求。 (請先閱讀背面之注意事項再填寫本頁) 本發月之目的也在提供—種新穎的製程，可以適合大量製造、且低 成本的印刷電路板生產電彳碑電極及感^!。 — 本發明另-目的是在提供_麵穎的電化學電極及感測器。 本發明更-目的是在提供—種卿的電化學雜及_器的各種應 用0 【發明之簡述】 3為使印刷電路板可以達到作為電化學分析用途的電極的要求，本發 月提供新穎的餘，可在標準的印刷電路板電路外層社所需厚度貴金 屬材料’並將電路板的導電電路底材完全的包覆。依本發明，新賴的製 程可以是雜同-基板上所㈣極的導電接點，於線路佈圖時就全部並 連至-電極。製備電化學電極時，再將所需厚度的貴金屬電極材料錄在 電路底材上。依此方法底材可完全被電極㈣所覆蓋，且縱切面不合有 底材稞露出來，達到將印刷電路的製程麟料應祕電化學電極的目 的0 經濟部中央標隼局員工消費合作社印製 依本發明製成之電化學電極及感测器適用於電極'感測器、生 測器、可絲式制以、及錄抑等方面的應^ . 上述及其他本發明之目的及優點，可由以下說明並參照 更形清楚。 口八@ 【圖式說明】 5 ( CNS ) A4im ( 210 X 297^" 496110 A7 B7 五、發明説明（i/ ) 第1圖顯示-種適用在本發明之印刷電路板電化學感測器之電極佈 局示意圖。 第2圖顯示本發明之電化學感測器應用在過氧化氣電流計之應用例 示意圖。 圖 圖 第3圖顯林㈣電化學細n細在魏化氫電流狀反應曲線 第4圖顯示本發明電化學感測韻用在赤▲奴度計之反應曲線 經濟部中央標準局員工消費合作祍印袈 第5圖顯示本發明電化學感測器應用在血糖試片之結構示音圖。 第6圖表示本發明應用於血糖試片對葡萄糖濃專反應曲線圖。 第7圖表示本發明應用於血糖試片詈測全血結果與自動化分析儀結 果的相關性不意圖。 【發明之詳細說明】 以下詳細說明以電鍍法製作印刷電路板電極之製程。更進一步的說 明包括印刷電路板電極在過氧化氫'赤血鹽等電化學分析的應用，在葡 萄糖生物感測器及血糖試片的應用。以下說明本發明之實施例。 【實施例】 本發明所揭示之印刷電路板電極製程，可以適用於任何習知之單面 或雙面電路板製程，適用的基材材料包括紙基材、鋼基板、複合基板、 玻璃布銅錄板、軟/硬m基板科。製程可包括加成法或減成 法 本實施例在製作印刷電路板電化學電極時，可將許多單個電極排在 同-電路基板上，並將電極的電氣接點全部並聯至_電極。第—圖顯示 私紙張尺度適用巾國國家標举-(CNS ) A4規格（21〇χ297公慶） f請先閲讀背面之注意事項再填寫本頁〕496110 A7 printed by the Consumer Cooperative of the Central Bureau of Standards of the Ministry of Economic Affairs ____________ B7 V. Description of the invention (丨) [Application scope of the invention] The present invention relates to an electrochemical sensor, especially a material and manufacturing process using printed circuit boards Therefore, it can be used as an electrode element of an electrochemical sensor. [Background of the invention] According to the printed circuit board (Printed Wiring Board or Printed Circuit Board; PWB or PCB), the electrical wiring connecting the circuit parts is drawn into a wiring pattern according to the circuit design, and then a specific processing method is used on the insulating substrate. A circuit board made up of electrical conductors. Its main function is to connect various electronic parts through the electronic circuit formed on the circuit board, so as to achieve the purpose of relay transmission and play the functions of various electronic components. The printed circuit board processes used are additive process and subtractive process. 0 Additive method refers to the method of chemical reaction on the surface of an insulated circuit substrate to generate the remainder of the conductive circuit. . The subtractive method refers to the process of making a circuit board with a conductive surface layer by chemically removing the conductor that partially collapses on the surface to form a circuit board. As indicated in the specification of the US Patent No. 5437 '(ElectrGdiemicai Sensor), fresh printed circuit boards * are suitable for use as electrochemical analysis electrodes, which is a well-known concept. This is due to the fact that the conductive surface of the printed circuit is generally high-reactive materials, such as conductive metals such as steel and nickel, in order to meet the requirements of easy processing, mass production, simplified manufacturing processes, and low prices. However, these highly reactive materials are easy to react with air or other materials during storage, which makes it difficult to control the surface properties. When in use, it is also easy to discontinue the reaction products and cause interference, so they cannot be used as Wei credits. Fine electricity selection. In order to improve the stability of the surface properties mentioned above, printed circuit boards use non-conductive materials, such as insulating green paint, or conductive materials with low reactivity, such as gold, platinum, 3 210X 297 mm) (please (Please read the precautions on the back before filling this page). · Order itquan 496110 A7 --------- V. Description of the invention (Λ) ----- = Two ΓΓ conductive metal circuit coating to prevent conductive metal substrate And air or its process 'really effectively solved the stability of the nature of the surface of the printed circuit board, but the quality of the printed circuit board was returned from the following day' while maintaining long-term stability. Please note this page before filling out this page ) Quasi-printing • Road board 7 knows that “Even if the surface layer has been plated with a stable and high-conducting gold material, standard electrical products, there is no requirement for Wei Zuowei's chemical polarization separation. There is a process for the outer conductive layer of printed circuit boards. ： Chemical immersion method and electric bond method 7 Miscellaneous materials use the high reactivity of copper and copper, which is different from precious metals such as gold, platinum, and stable readings. 'The copper and nickel circuit boards are made by precious metal ion electrolysis. Of ~ = learn from The t metal is deposited on the substrate, so that the substrate can be precious gold = Wang cover 1. Since this method can only be used on the substrate, the metal is separated from the finished substrate, which is used in the application of electrochemical electrodes. 'It is still susceptible to interference during measurement, so it cannot be used as an electrode for electrochemical analysis. Electric money method: It is a conventional outer layer process of printed circuit board circuits, which can get thicker = gold layer', but after removing the photoresist and When the substrate is used, the substrate will be exposed on the longitudinal section of the edge of the line. = When the electrode is an electrochemical reaction electrode, the substrate will react with the test object. In the application of electrochemical electricity, it will be disturbed during measurement. Electrode for electrochemical analysis. In order to achieve the purpose of electrochemical electrode application, U.S. Patent No. 54379㈣ discloses a method of making a precious metal film on an insulating substrate by physical / child product method, and then sticking it on a hard or soft substrate In order to obtain a working electrode suitable for electrochemical applications, as for the counter electrode or reference electrode, a standard printed circuit board is used. This method can theoretically solve the above problems, but its process is complicated, and it is produced by physical deposition. The cost of the precious metal film is high, which is inconsistent with the original intention of using printed circuit 2 as an electrode to reduce costs. [Objective of the invention] The national standard of this paper rule (CNs y A4 specification (A7 B7 V. Description of the invention (3)) The purpose is to provide a novel process, which can be plated with a metal material of the thickness of a standard printed circuit board, so that the printed circuit board can meet the specifications of electrochemical electrodes and sensors. (Please read first Note on the back, please fill in this page again) The purpose of this month is also to provide a novel process, which can be suitable for mass production and low cost printed circuit board production of electric stele electrodes and sensors ^ !. — This invention also- The purpose is to provide the electrochemical electrode and sensor of the surface. The present invention is more-the purpose is to provide-the various applications of the electrochemical hybrid device of the Qingqing 0 [Brief description of the invention] 3 for the printed circuit board Can meet the requirements of electrodes for electrochemical analysis, this month provides a novel spare, can be used in standard printed circuit board circuit outer layer of the required thickness of precious metal materials' and the circuit board's Completely covering the electrical circuit substrate. According to the present invention, Xinlai's process can be the conductive contacts of the electrodes on the hetero-substrate, which are all connected to the-electrode in parallel when the circuit is laid out. When preparing an electrochemical electrode, a noble metal electrode material of a desired thickness is recorded on a circuit substrate. According to this method, the substrate can be completely covered by the electrode ，, and the longitudinal section is not exposed with the substrate 稞, so as to achieve the purpose of secreting the electrochemical electrode in the printed circuit manufacturing process. The electrochemical electrode and sensor made in accordance with the present invention are applicable to electrode 'sensors, biosensors, wire-type and recording suppression, etc .. The above and other objects and advantages of the present invention, It will be clearer from the following description and reference.口 八 @ [Schematic description] 5 (CNS) A4im (210 X 297 ^ " 496110 A7 B7 V. Description of the invention (i /) Figure 1 shows a kind of electrochemical sensor suitable for the printed circuit board of the present invention Schematic diagram of electrode layout. Fig. 2 shows an application example of the electrochemical sensor of the present invention applied to a peroxide gas galvanometer. Fig. Fig. 3 shows the current response curve of Lin Wei's electrochemical n-thin in Weihua hydrogen current-like reaction curve. The figure shows the response curve of the electrochemical sensing rhyme of the present invention used in a red ▲ slave meter. The consumer cooperation of the Central Standards Bureau of the Ministry of Economic Affairs 祍 印 袈 Figure 5 shows the structure of the electrochemical sensor of the present invention applied to a blood glucose test strip. Fig. 6 is a graph showing the response curve of glucose concentration test to glucose concentration of the present invention. Fig. 7 is a graph showing the correlation between the result of the whole blood test and the result of an automatic analyzer applied to the blood glucose test strip. [Invention Detailed description] The following is a detailed description of the process of making printed circuit board electrodes by electroplating. Further explanation includes the application of printed circuit board electrodes in the electrochemical analysis of hydrogen peroxide, red blood salt, etc. Application of measuring device and blood glucose test strip. The following describes the embodiment of the present invention. [Example] The printed circuit board electrode manufacturing process disclosed in the present invention can be applied to any conventional single-sided or double-sided circuit board manufacturing process. Materials include paper substrates, steel substrates, composite substrates, glass cloth copper recording boards, and soft / hard substrates. The manufacturing process can include addition or subtraction methods. In this embodiment, when manufacturing electrochemical electrodes for printed circuit boards, Many single electrodes are arranged on the same circuit board, and all the electrical contacts of the electrodes are connected in parallel to the _electrode. The figure shows the national paper standard for private paper scale application (CNS) A4 specification (21〇297297 ) F Please read the notes on the back before filling in this page]
496110 A7 B7 五、發明説明（f ) 一種適用在本發明之印刷電路板電化學電極佈局示意圖。如圖所示，一 片印刷電路板（1)上可配布數十至數百個電化學電極（2)，其所有接點 均並聯至一接點（3)。 首先製備一印刷電路板基材（1)，該基材（1)可為任何金屬，通常係 使用銅或鎳。其次，將該基材之線路部份以光阻劑（未圖示）覆蓋。在 經過曝光、顯影、侧、去光阻劑等印刷電路板的標準製程後，鋼猪印 刷電路板電化學電極就精確的在基板⑴形成。在本實細也可使用加 成的方法直接在沒有銅箔的基材（1)上製作鋼箔電路。接著，利用連接 在-起的接點⑶進行中間層及電極㈣的電鍍1處之中間層材料 包括錄、把等及其它適合的導電材料；電極材料則可包括碳及金、銀、 始、纪等及其它適合的導電㈣。魏之方式可以湘任何習知之電鑛 法。經過電鍍之後電路之銅箱及中間層縱切面也會鑛上電極材料，且: 厚度可以精確的㈣，更沒有厚度的關。因此可以將底材電路完整'包 覆’不致外露。最後，將製得之印刷電路板清洗，施予絕緣層，㈣後， 即可獲得品質-致的印刷電路板電化學電極（2)。切割時 聯之接點乾淨切除，以免在應用時產生不必要的短路。 '、 2上述實施例製得的成品，可以經由習知的方法·鍍層的 經濟部中央標準局員工消費合作社印製 (請先閲讀背面之注意事項再填寫本頁) 其方法包括x_光測厚儀’電化_厚度法二 :=了_品質的方法，也是電錢工 ； 的習用方法。在此均不贅述。 包观口口貝 财法製狀電化學電極可叫料各種 測用途。以下分別舉例說明。 电1匕予α 的應用 496110 A7 B7 五、發明説明（4 ) 過氧化氫是一還原劑，它是許多氧化酵素反應的產物，常作為檢驗 分析的用途。在電化學分析，過氧化氫可以用電流計法分析。第2圖顯 不本發明之電化學電極在過氧化氫感測器之應用。如第2圖所示，將過 氧化氫感測器（2)接在定電位分析儀（4)上，並將分析電位設定在例 如650mV。再將過氧化氫感測器（2)固定於反應器（5)，在反應器（5) 裡加入2毫升的緩衝液。啟動分析，等到背景電流穩定後，加入〇· i毫 升的過氧化氫樣品，以記錄器記錄其反應電流的大小。第3圖顯 不本發明過氧化氫感測器濃度反應曲線圖。如圖所示，其分析的線性範 圍為ImM到10mM，其敏感度為 應用例二、赤血釀感測装的處甲 赤血鹽是一氧化還原劑，是許多氧化酵素反應的電子媒介物，常被 用在生物感測的用途。在電化學分析可以用電流計法分析，其配置亦 如第2圖所π。量測時將赤血鹽感測器⑵接在定電位分析儀⑷上， 並將分析電位設定在350 mV，將電極⑵固定於反應器⑸，在反應 益（5)裡加人2毫升的緩衝液。啟動分析，等到背景電流穩定後，加 入0· 1笔升的樣品，以記錄器（6)記錄其反應電流的大小。第4圖顯 示本發明赤血鹽感測器的濃度反應曲線圖。如圖所示，其分析的線性範 圍為0·01πιΜ到ImM，其敏感度為〇 〇92/zA/m。 經濟部中央榡準局員工消費合作，社印製 0 ^ II (請先閲讀背面之注意事項再填寫本頁)496110 A7 B7 V. Description of the invention (f) A schematic diagram of an electrochemical electrode layout of a printed circuit board suitable for the present invention. As shown in the figure, a printed circuit board (1) can be equipped with dozens to hundreds of electrochemical electrodes (2), and all the contacts are connected in parallel to one contact (3). First, a printed circuit board substrate (1) is prepared. The substrate (1) can be any metal, and usually copper or nickel is used. Second, the circuit portion of the substrate is covered with a photoresist (not shown). After standard processes of printed circuit boards such as exposure, development, side and photoresist removal, the electrochemical electrodes of the steel pig printed circuit board are accurately formed on the substrate. In the present case, a steel foil circuit can also be produced directly on a substrate (1) without a copper foil by an addition method. Next, the intermediate layer and electrode ㈣ are electroplated by using the contact ⑶ connected at the bottom. The intermediate layer materials include recording, handles, and other suitable conductive materials; the electrode materials can include carbon and gold, silver, starting, Ji et al. And other suitable conductive plutonium. Wei's method can be used in any conventional electric ore method. After the electroplating of the copper box and the middle layer of the circuit, the electrode material will be mined on the longitudinal section of the circuit, and: the thickness can be accurately measured, and there is no thickness. Therefore, the substrate circuit can be completely 'covered' without being exposed. Finally, the prepared printed circuit board is cleaned and an insulating layer is applied. After that, a uniform quality printed circuit board electrochemical electrode (2) can be obtained. When cutting, the joints are cleanly removed to avoid unnecessary short circuits during application. ', 2 The finished product prepared in the above examples can be printed by a conventional method · Plating of the Consumer Standards Cooperative of the Central Bureau of Standards of the Ministry of Economic Affairs (please read the precautions on the back before filling this page) The method includes x_ 光 测Houyi's electrification_thickness method two: = the method of _ quality, is also the customary method of electric money. I will not repeat them here. Baoguan Mouth Mouth The electrochemical method of electrochemical electrodes can be used for a variety of applications. Examples are given below. Application of electricity 1 to α 496110 A7 B7 V. Description of the invention (4) Hydrogen peroxide is a reducing agent, which is the product of many oxidative enzyme reactions, and is often used for inspection and analysis. In electrochemical analysis, hydrogen peroxide can be analyzed by galvanometry. Figure 2 shows the application of the electrochemical electrode of the present invention to a hydrogen peroxide sensor. As shown in Figure 2, connect the hydrogen peroxide sensor (2) to the potentiostat (4), and set the analysis potential to, for example, 650mV. Then fix the hydrogen peroxide sensor (2) to the reactor (5), and add 2 ml of buffer into the reactor (5). Start the analysis, wait until the background current is stable, and add 0.1 mL of hydrogen peroxide sample to record the reaction current with a recorder. Fig. 3 shows the concentration response curve of the hydrogen peroxide sensor according to the present invention. As shown in the figure, the linear range of its analysis is ImM to 10 mM, and its sensitivity is Application Example 2. The red blood salt of virion red blood is a redox agent and is an electronic mediator for many oxidase reactions. , Often used in biosensing applications. The electrochemical analysis can be performed by the galvanometer method, and its configuration is as shown in Fig. 2π. During the measurement, connect the red blood salt sensor ⑵ to the potentiostat ⑷, and set the analysis potential to 350 mV. Fix the electrode 于 to the reactor ⑸, and add 2 ml of the reaction benefit (5). Buffer. Start the analysis, wait until the background current is stable, add a 0.1 liter sample, and record the magnitude of the reaction current with the recorder (6). Figure 4 shows the concentration response curve of the red blood salt sensor of the present invention. As shown in the figure, the linear range of its analysis is from 0.01 μm to ImM, and its sensitivity is 〇92 / zA / m. Printed by the agency of the Central Procurement Bureau of the Ministry of Economic Affairs 0 ^ II (Please read the precautions on the back before filling this page)
、1T 應屋_例三二血後毯片的應用 第5圖顯示本發明電化學感測器應用在血糖試片之結構示意圖。如 第5圖所示’印刷電路板電化學電極⑵具有—基板⑼）、一銅底 材⑵）+鎳底材（22) ’其上包覆電極導電層（23)及絕緣材料層 (24 )。將葡萄糖氧化酵素、牛血清蛋白及赤血独緩衝液配製成反應 k紙張^適用中國—國家規格 'X 297公釐） 496110 A7 B7 五'發明説明（7) 液（25)，將反應液均勻的塗佈於本發明的電化學電極（？），等配方降 乾後，即製成血糖生物感測器。在感測區兩側以雙面膠帶（26)貼上塑膠 片（27)，如此便在試片的感測區完成了定量的毛細細管通道（28)。〆 企糖的量測，是在450 mV的極化電壓條件下，利用毛細現象將樣 品吸入，並量測其一定反應時間後的電流值，經由運算可得到樣品的血 糖值。第6圖顯示以血糖試片量測不同濃度葡萄糖的結果，在卜2微 升樣品量條件下，本發明電化學感測器的敏感度為6〇 nA/(mg/dl)葡萄 糖，可量測的濃度範圍為〇-60〇mg/dL葡萄糖，量測的精確度為cv<5%， 批次間的特性差異也是在一定的範圍。第7圖更進一步的將血糖試片^ 測全血的結果與臨床自動分析儀的結果的比較，由結果可見兩種方法的 相關係數為0. 9999。 其他適合本發明在生物感測器的應用，包括酵素、免疫、基因探針 及其它經過生物活性物質反應後，其反應物或產物可以經由電化學^析 而得知的所有物質的分析的應用。 【發明之效果】 技術方面：本發明可以在印刷電路板上鍍厚磨转厚的導電層（電極 材料），且可以將底材完全的覆蓋，避免底材與檢測物反應，以達到應 用於電化學電極的目的。 經濟部中央標準局員工消費合阼fi-v千贤 (請先閲讀背面之注意事項再填寫本頁) 應用方面：印刷電路板的材料與製程在以前是無法應用於電化學電 極，經由本發明此困難已被克服，這使印刷電路板可以被應用於電化學 電極及更多的延伸的應用。 效益方面：本發明不僅可以應用於目標物，血糖試片，更可以應用 於其它電化學分析。就血糖試片的應用來說就已經具有足夠的經濟^ 9 本紙張尺度適用中國國家標準（CNS ) A4規格（210X 297公釐） 496110 A7 B7 五、發明説明（f ) 盈’未來開發更多的產品，其效益也就越高。 以上是對本發明印刷電路板電化學感測器之實施例之説明’習於斯 藝之人士由上狀說明，日崎本發明讀神進而作料_衍伸與 變化’唯只要不超出本發明之精神，均應包含於其申請專利範圍之内。 (請先閱讀背面之注意事項再填寫本頁} .袈- 經濟部中央標隼局員工消費合作社印製 10 本纸張尺度適用中國國家蘇_ ( CNS ) A4規格（270^297^¾-、 1T Yingwu_ Example 32 Application of the post-blood blanket sheet Figure 5 shows a schematic structural diagram of the application of the electrochemical sensor of the present invention to a blood glucose test strip. As shown in FIG. 5 'the electrochemical electrode of the printed circuit board ⑵ has a substrate ⑼), a copper substrate ⑵) + a nickel substrate (22)' which is covered with an electrode conductive layer (23) and an insulating material layer (24 ). Glucose oxidizing enzyme, bovine serum protein and red blood alone buffer solution are prepared into reaction k paper ^ Applicable to China-national specification 'X 297 mm) 496110 A7 B7 Five' invention description (7) solution (25), the reaction solution The electrochemical electrode (?) Of the present invention is uniformly coated, and the blood glucose biosensor is prepared after the formula is dried. A plastic sheet (27) is affixed on both sides of the sensing area with double-sided tape (26), so that a quantitative capillary channel (28) is completed in the sensing area of the test piece. 〆 The measurement of enterprise sugar is to take in the sample under the condition of polarization voltage of 450 mV, and use the capillary phenomenon to measure the current value after a certain reaction time. The blood sugar value of the sample can be obtained through calculation. Figure 6 shows the results of measuring different concentrations of glucose with blood glucose test strips. Under the condition of 2 microliters of sample volume, the sensitivity of the electrochemical sensor of the present invention is 60 nA / (mg / dl) glucose, which can be measured. The measured concentration range is 0 to 60 mg / dL glucose, the measurement accuracy is cv < 5%, and the characteristic difference between batches is also in a certain range. Figure 7 further compares the results of the blood glucose test strip ^ whole blood measurement with the results of the clinical automatic analyzer. From the results, it can be seen that the correlation coefficient of the two methods is 0.99999. Other applications suitable for the biosensor of the present invention include the analysis of enzymes, immunity, genetic probes, and other substances whose reaction or products can be learned by electrochemical analysis after reacting with biologically active substances . [Effects of the invention] Technical aspect: The present invention can plate a thick-grind conductive layer (electrode material) on a printed circuit board, and can completely cover the substrate, avoiding the reaction between the substrate and the detection object, so as to achieve application Purpose of electrochemical electrodes. Employees of the Central Bureau of Standards of the Ministry of Economic Affairs, Fi-v Qianxian (please read the precautions on the back before filling this page) Application: The materials and processes of printed circuit boards could not be applied to electrochemical electrodes before. This difficulty has been overcome, which allows printed circuit boards to be applied to electrochemical electrodes and more extended applications. In terms of benefits: the present invention can be applied not only to the target substance, the blood glucose test strip, but also to other electrochemical analysis. It is already economical enough for the application of blood glucose test strips ^ 9 This paper size is applicable to the Chinese National Standard (CNS) A4 specification (210X 297 mm) 496110 A7 B7 V. Description of the invention (f) Profit 'Future development more Products, the higher the effectiveness. The above is an explanation of the embodiment of the printed circuit board electrochemical sensor of the present invention. "People who are accustomed to Siyi will explain from the above description. Hizaki's invention reads the god and then makes the material _ extension and change." As long as it does not exceed the invention The spirit should be included in the scope of its patent application. (Please read the notes on the back before filling out this page}. 袈-Printed by the Consumer Cooperatives of the Central Bureau of Standards, Ministry of Economic Affairs 10 This paper size is applicable to the Chinese National Su_ (CNS) A4 specification (270 ^ 297 ^ ¾-