TW202214835A - Detection chip nucleic acid detection disc and nucleic acid detection - Google Patents

Abstract

A detection chip comprises a first cover plate, a second cover plate, a conductive part and two first driving electrodes. The conductive part comprises a first surface and a second surface which are arranged opposite to each other. The first surface and the second surface are respectively adjacent to the first cover plate and the second cover plate to form a channel. The channel comprises a detection path. The channel is used to carry a detection liquid, and the detection liquid is charged. The two first driving electrodes are electrically connected with the conductive part. After the conductive part is electrified, a driving force is generated between the conductive part and the detection liquid, which is used to drive the detection liquid to move away from the conductive part. The detection chip can prevent the detection liquid from accidentally leaving the detection path and adsorbing on the side wall of the conductive part, to ensure the normal amplification reaction. A nucleic acid detection disc and a nucleic acid detection device are also provided.

Description

Detection chip, nucleic acid detection box and nucleic acid detection equipment

The invention relates to a detection chip, a nucleic acid detection box and nucleic acid detection equipment.

At present, most of the detections for molecular diagnosis, morphology, immunology, etc. are carried out using microfluidic detection chips.

The structure of the conventional detection chip includes an upper cover plate, a lower cover plate and a spacer layer between the upper and lower cover plates, wherein the upper cover plate, the spacer layer and the lower cover plate together form a channel for carrying the detection liquid. During the detection process, the detection liquid droplets will move in different areas in the channel to complete the nucleic acid amplification process. However, when operating problems or other factors cause the liquid droplets to be adsorbed on the sidewall of the spacer layer due to electrostatic action, it will be The droplet cannot move in the specified path, causing the experiment to fail

In view of this, in order to overcome at least one of the above-mentioned defects, it is necessary to provide a detection chip.

In addition, the present application also provides a nucleic acid detection box and a nucleic acid detection device comprising the above-mentioned detection chip.

The present invention provides a detection chip, which includes: a first cover plate, a second cover plate, a conductive part and two first driving electrodes. The conductive part includes a first surface and a second surface disposed opposite to each other, the first surface and the second surface are respectively adjacent to the first cover plate and the second cover plate, the first cover plate , the conductive part and the second cover plate are surrounded to form a channel, the channel includes a detection path, the channel is used to carry a detection liquid, and the detection liquid is charged. Both of the two first driving electrodes are electrically connected to the conductive portion, and are used to energize or de-energize the conductive portion. Wherein, a driving force is generated between the conductive part and the detection liquid after electrification, and the driving force is used to drive the detection liquid to move in a direction away from the conductive part, so that the detection liquid moves to the desired position. on the detection path.

In the embodiment of the present application, the first driving electrode is disposed between the first surface and the first cover plate or the second cover plate, and the first driving electrode and the first surface are electrically connected to each other. sexual connection.

In the embodiment of the present application, the first cover plate is provided with two first grooves corresponding to the first surface, and each of the first grooves is provided with one of the first driving electrodes, corresponding to two The first surface of the first groove protrudes toward the direction away from the second surface to form two first protrusions, each of the first protrusions is accommodated in a corresponding one of the first recesses inside the groove, and the first bump is electrically connected to the first driving electrode.

In the embodiment of the present application, the first cover plate is provided with a first groove corresponding to the first surface, the second cover plate is provided with a second groove corresponding to the second surface, and the first cover plate is provided with a second groove corresponding to the second surface. One of the first driving electrodes is arranged in both the groove and the second groove. The first surface protrudes toward the direction away from the second surface to form a first bump, the first bump is accommodated in the first groove, and the first bump is connected to a The first driving electrodes are electrically connected. The second surface protrudes toward the direction away from the first surface to form a second bump, the second bump is accommodated in the second groove, and the second bump and the other The first driving electrodes are electrically connected.

In an embodiment of the present application, the conductive portion is provided with two third grooves, and the two first driving electrodes are respectively disposed in the two third grooves and are electrically connected to the conductive portion.

In the embodiment of the present application, the conductive part includes a conductive part body and a first conductive layer disposed on a surface of the conductive part body on one side of the body close to the channel, the first conductive layer and the two first drivers Electrodes are electrically connected.

In the embodiment of the present application, the detection chip further includes a drive element, and the drive element includes a drive circuit disposed on a surface of the first cover plate close to the second cover plate, a drive circuit disposed on the second cover The second conductive layer on the side of the board close to the first cover plate, the first dielectric layer disposed on the side of the driving circuit close to the second cover plate, and the second conductive layer disposed on the side of the second cover plate close to the first cover plate. A second dielectric layer on one side of the cover plate, the channel is formed between the first dielectric layer and the second dielectric layer, and the detection path is formed by the driving circuit.

In the embodiment of the present application, the driving circuit includes a plurality of second driving electrodes arranged in an array and a control electrode electrically connected to all the second driving electrodes, and the second driving electrodes are electrically connected to the second driving electrodes. The electrification or de-energization between the layers is used to drive the detection liquid to move between two adjacent second drive electrodes.

The present invention also provides a nucleic acid detection box, which includes a box body, a detection chip and a connector. The detection chip is disposed in the box body, and the detection chip is the detection chip as described above. The connector is electrically connected to the driving element and the first driving electrode, respectively. Wherein, the detection chip is used for performing nucleic acid amplification reaction on the detection solution to obtain nucleic acid amplification products.

The present invention also provides a nucleic acid detection device, which includes a host and a detection box installation slot. The detection box installation slot is provided on the host, and the detection box installation slot is used to detachably install the nucleic acid detection box, and the nucleic acid detection box is the nucleic acid detection box as described above.

Compared with the prior art, the detection chip provided by the present invention has a simple structure design and is easy to assemble. By means of the design of the conductive part and the first driving electrode, the detection liquid adsorbed on the side wall of the conductive part can be returned to the detection path, thereby ensuring The normal progress of the nucleic acid amplification reaction.

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all the embodiments.

It should be noted that when an element is referred to as being "fixed to" another element, it can be directly on the other element or intervening elements may also be present. When an element is referred to as being "connected" to another element, it can be directly connected to the other element or intervening elements may also be present. When an element is referred to as being "disposed on" another element, it can be directly disposed on the other element or intervening elements may also be present. The terms "vertical," "horizontal," "left," "right," and similar expressions are used herein for illustrative purposes only.

The system implementations described below are only illustrative, and the division of the modules or circuits is only a logical function division, and other division methods may be used in actual implementation. Furthermore, it is clear that the word "comprising" does not exclude other units or steps and the singular does not exclude the plural. Multiple units or means stated in the system claim may also be implemented by the same unit or means through software or hardware. The terms first, second, etc. are used to denote names and do not denote any particular order.

Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. The terms used herein in the description of the present invention are for the purpose of describing specific embodiments only, and are not intended to limit the present invention. As used herein, the term "and/or" includes any and all combinations of one or more of the associated listed items.

Please refer to FIGS. 1 to 3 , which are a detection chip 100 provided in an embodiment of the present invention. The detection chip 100 includes a first cover plate 1 , a second cover plate 2 , a conductive portion 3 and two first driving electrodes 4 . The conductive portion 3 includes a first surface 31 and a second surface 32 disposed opposite to each other, and the first surface 31 and the second surface 32 are respectively disposed adjacent to the first cover plate 1 and the second cover plate 2, The first cover plate 1 , the conductive portion 3 and the second cover plate 2 are surrounded to form a channel 5 , the channel 5 includes a detection path 6 , and the channel 5 is used to carry a detection liquid 7 , and the detection liquid 7 is charged . The two first driving electrodes 4 are both electrically connected to the conductive portion 3 for energizing or de-energizing the conductive portion 3 . Wherein, a driving force is generated between the conductive part 3 and the detection liquid 7 after being energized, and the driving force is used to drive the detection liquid 7 to move in a direction away from the conductive part 3, so that the detection liquid 7 moves to the detection liquid 7 on path 6. The detection chip 100 is used for nucleic acid amplification reaction, and the detection solution 7 containing the nucleic acid sample is added into the channel 5 . It should be noted that the detection solution 7 exists in the channel 5 in the form of liquid beads.

Please refer to FIG. 2 and FIG. 3 , the detection chip 100 further includes a driving element 9 . The driving element 9 includes a driving circuit 91 disposed on the side of the first cover plate 1 close to the second cover plate 2 , and disposed in the driving circuit 91 The first dielectric layer 92 on the side close to the second cover plate 2 , the second conductive layer 93 disposed on the side of the second cover plate 2 close to the first cover plate 1 , and the second conductive layer 93 The second dielectric layer 94 on the side close to the first cover plate 1, wherein the channel 5 is formed between the first dielectric layer 92 and the second dielectric layer 94, and the driving circuit 91 forms the Detect path 6. By energizing or de-energizing the driving circuit 91 and the second conductive layer 93, the detection liquid 7 can be moved in the channel 5 according to the detection path 6 specified above.

In this embodiment, as shown in FIG. 2 and FIG. 3 , the driving circuit 91 includes a plurality of second driving electrodes 911 arranged in an array and a control electrode 912 electrically connected to all the second driving electrodes 911 . Specifically, the driving circuit 91 is a thin film transistor (Thin Film Transistor, TFT) driving circuit, and because the detection liquid 7 has conductivity, combined with the principle of dielectric wetting (Electrowitting-On-Dielectric, EWOD), the detection liquid can be realized. 7 moves along the predetermined detection path 6 in the channel 5 . Using the TFT principle, the circuit between a certain second driving electrode 911 and the second conductive layer 93 can be selectively turned on or off, thereby changing the voltage between the second driving electrode 911 and the second conductive layer 93 to change the detection The wetting characteristics between the liquid 7 and the first dielectric layer 92 and the second dielectric layer 92 further control the movement of the detection liquid 7 in the channel 5 according to the predetermined detection path 6 . As shown in FIG. 2, the detection liquid 7 moves on the electrode I, the electrode H and the electrode G. When the detection liquid 7 is on the electrode H, a voltage is applied between the electrode G and the second conductive layer 93, and the voltage Vd is given to the electrode G. At the same time, the voltage between the electrode H and the second conductive layer 93 is disconnected, and the wetting characteristics between the detection liquid 7 and the first dielectric layer 92 and the second dielectric layer 94 are changed, so that the electrode H and the second dielectric layer 94 are changed. The liquid-solid contact angle between the detection liquid 7 becomes larger, and the liquid-solid contact angle between the electrode G and the detection liquid 7 becomes smaller, so that the detection liquid 7 moves from the electrode H to the electrode G.

In this embodiment, the first dielectric layer 92 and the second dielectric layer 94 are both insulating and hydrophobic layers, specifically, a polytetrafluoroethylene coating. On the one hand, it can play an insulating and hydrophobic role, and on the other hand It can make the detection liquid 7 move more smoothly in the predetermined path, and prevent the liquid droplets from breaking during the movement.

In this embodiment, referring to FIG. 2 , the driving circuit 91 is disposed on the surface of the first cover 1 close to the channel 5 . Specifically, the driving circuit 91 may be formed by a metal etching method or an electroplating method.

In this embodiment, referring to FIG. 3 , the control electrode 912 is integrated on the same edge of the first cover plate 1 , which can facilitate the connection between the control electrode 912 and the connector.

Referring to FIG. 3 , the driving circuit 91 can be divided into multiple areas according to different uses, namely a sample adding area A, a reagent storage area B, a plurality of nucleic acid amplification areas C and a liquid discharge area D. The second cover plate 2 is also provided with a sample addition tank 22 corresponding to the sample addition area A, the sample addition tank 22 is communicated with the sample addition area A, and the detection liquid is added to the sample addition area A from the sample addition tank 22 7. The reagent storage area B is used to store fluorescent reagents (such as fluorescent dyes or fluorescent probes). The detection solution 7 performs nucleic acid amplification reaction in the nucleic acid amplification region C. The nucleic acid amplification region C may include multiple regions, and the number of specific regions may be determined according to actual detection requirements.

Please refer to FIG. 2 and FIG. 3 , the specific movement path of the detection liquid 7 in the detection chip 100 is: after the detection liquid 7 enters the sample adding area A, it moves to the nucleic acid amplification area C according to a predetermined path under the driving of the second driving electrode 911 Carry out the amplification reaction; when the amplification reaction is completed, the amplified product moves to the reagent storage area B to be mixed with the fluorescent reagent, thereby obtaining the nucleic acid amplification product combined with the fluorescent reagent; the uniformly mixed nucleic acid amplification product is in the first step. The two driving electrodes 911 move to the liquid outlet area D to enter the next step of detection. During the movement of the detection liquid 7 on the detection path 6, if the detection liquid 7 is adsorbed on the side wall of the conductive part 3 due to operation problems or electrostatic attraction, the negative charge of the conductive part 3 will replace the positive charge of the detection liquid 7. Neutralized, so that the detection liquid 7 is completely negatively charged, so that a repulsive driving force will be formed between the detection liquid 7 and the conductive part 3, and the detection liquid 7 is driven away from the side wall of the conductive part 3 under the action of the repulsive force, and again Return to the detection path 6, so as to ensure the normal progress of the nucleic acid amplification reaction. The design of the conductive part 3 and the first driving electrode 4 of the present invention cleverly utilizes the principle of repulsion of the same charge, which solves the problem that the detection solution will accidentally leave the detection path and the amplification reaction cannot be performed normally.

The electrical connection between the first driving electrode 4 and the conductive portion 3 can be realized in various ways. Referring to FIG. 2 , in this embodiment, the first driving electrode 4 is disposed between the first cover plate 1 and the first surface 31 of the conductive portion 3 , and the first driving electrode 4 is in contact with the conductive portion 3 and is electrically For connection, in order to ensure the tightness of the channel 5 , a sealing material can be filled between the first surface 31 and the surface of the first cover plate 1 close to the second cover plate 2 . In this embodiment, the installation of the first driving electrode 4 and the connection with the external power supply are relatively easy to implement.

In this embodiment, an opening 95 is formed on the first dielectric layer 92 , the first driving electrode 4 is embedded in the opening 95 , and the two opposite surfaces of the first driving electrode 4 are respectively the first surface 31 is in contact with the surface of the first cover plate 1 close to the second cover plate 2 , and the first dielectric layer 92 fills the gap between the first surface 31 and the first cover plate 1 , so as to realize the sealing of the channel 5 .

In this embodiment, in order to make the first driving electrode 4 fully contact with the first surface 31 and ensure the stability of the electrical connection, the first surface 31 can be provided with a raised portion 33, and the raised portion 33 is embedded in the opening In 95, the contact and electrical connection with the first driving electrode 4 is realized.

In this embodiment, the first driving electrode 4 may be an electrode sheet.

Referring to FIG. 4 , in the second embodiment, two first grooves 11 are formed in the area of the first cover 1 in contact with the first surface 31 , and each of the first grooves 11 is provided with two first grooves 11 . One of the first driving electrodes 4, corresponding to the first surfaces 31 of the two first grooves 11, protrudes toward the direction away from the second surface 32 to form two first bumps 34, each of the first bumps 34 is accommodated in a corresponding one of the first grooves 11 , and the first bumps 34 are in contact with and electrically connected to the first driving electrodes 4 located in the first grooves 11 . That is, the first cover 1 is provided with two first grooves 11 for accommodating the first driving electrodes 4 , and then the conductive parts 3 and the first driving electrodes are realized by placing the first bumps 34 on the conductive parts 3 4 contacts and electrical connections between. In this embodiment, accommodating the first driving electrode 4 in the first groove 11 of the first cover plate 1 will not affect the sealing performance of the channel 5 . The connection stability is better.

In this embodiment, the first driving electrode 4 is an electrode sheet.

Referring to FIG. 5 , in the third embodiment, a first groove 11 is provided in the area where the first cover 1 is in contact with the first surface 31 , and the second cover 2 and the second The area where the surface 32 contacts is provided with a second groove 21 , wherein a first driving electrode 4 is provided in both the first groove 11 and the second groove 21 . In addition, the first surface 31 protrudes in a direction away from the second surface 32 to form a first bump 34 , the first bump 34 is accommodated in the first groove 11 , and the first bump 34 is The bump 34 is in contact with and electrically connected to a first driving electrode 4 . The second surface 32 protrudes away from the first surface 31 to form a second bump 35 , the second bump 35 is accommodated in the second groove 21 , and the second bump 35 is connected to another bump 35 . A first driving electrode 4 contacts and is electrically connected. In this embodiment, by disposing the first groove 11 and the second groove 21 on the first cover plate 1 and the second cover plate 2, respectively, the two first driving electrodes 4 are respectively accommodated in the grooves. The purpose of connecting the power supply to the upper and lower surfaces of the conductive part 3 can be achieved, so that a voltage can be applied to the conductive part 3 . In this embodiment, accommodating the first driving electrode 4 in the first groove 11 and the second groove 21 will not affect the sealing performance of the channel 5 , and the connection between the first driving electrode 4 and the conductive portion 3 The connection stability is better.

In this embodiment, the first driving electrode 4 is an electrode sheet.

Referring to FIG. 6 , in the fourth embodiment, the conductive portion 3 is provided with two third grooves 36 , and the two first driving electrodes 4 are respectively disposed in the two third grooves 36 and are connected to the two third grooves 36 . The conductive portion 3 is in contact and electrically connected. In this embodiment, by forming two third grooves 36 on the conductive portion 3 for accommodating the first driving electrodes 4 , the first cover plate 1 and the second cover plate 2 are avoided to be slotted, which is convenient for the first cover plate 1 and the second cover plate 2 The formation of the three grooves 36 facilitates the assembly of the detection chip 100 . In addition, the position of the third groove 36 on the conductive portion 3 can be specifically designed according to actual needs. Specifically, the opening of the third groove 36 can be set on the first surface 31 and/or the second surface 32. , can also be arranged on the side wall of the conductive part 3 away from the channel 5 .

In this embodiment, the opening of the third groove 36 is arranged on the side wall of the conductive portion 3 away from the channel 5 . This design is more convenient for the first driving electrode 4 to be electrically connected to the power supply, and will not affect the sealing of the channel 5 . sex.

In this embodiment, the first driving electrode 4 is an electrode sheet.

Referring to FIG. 7 , in the fifth embodiment, the conductive portion 3 includes a conductive portion body 37 and a first conductive layer 38 disposed on a surface of the conductive portion body 37 near the channel 5 . The first conductive layer 38 is connected to The two first driving electrodes 4 are electrically connected. In this embodiment, the conductive portion 3 does not need to be conductive in its entirety, and it is only necessary to coat or attach the first conductive layer 38 to the sidewall of the conductive portion body 37 close to the channel 5 . In addition, at this time, the first driving electrode 4 can be electrically connected to the first conductive layer 38 in any form, as long as the electrical connection between the first conductive layer 38 and the power source can be achieved and the sealing of the channel 5 can be ensured.

Referring to FIG. 8 , in the sixth embodiment, the first driving electrode 8 can also be a strip-shaped electrode with a certain aspect ratio, such as a needle-shaped or rod-shaped electrode, and one end of the first driving electrode 8 is fixed on the conductive part 3, and the other end is connected to the power supply. By using the first driving electrode 8 of this embodiment, there is no need to create a groove, which reduces the difficulty of forming and reduces the cost. In addition, the assembly is convenient, and the circuit of the conductive portion 3 is more convenient to lead out.

The two first driving electrodes (4, 8) of the present invention are respectively connected to the positive and negative poles of the power supply, and the conductive portion 3 is energized or powered off by the two first driving electrodes (4, 8). Specifically, as shown in FIG. 2 It is shown that the two first driving electrodes (4, 8) apply a negative voltage to the conductive part 3, so that the conductive part 3 is negatively charged after electrification, and the negative conductive part 3 adsorbs the detection liquid 7 under the action of electrostatic force to the side wall of the conductive part 3 close to the channel 5 . As shown in FIG. 9 , the negative charges on the conductive portion 3 and the positive charges of the detection liquid 7 are neutralized, so that the detection liquid 7 is only negatively charged. As shown in FIG. 10 , in conjunction with FIG. 3 , at this time, the same kind of charges repel each other, and the detection liquid 7 will be pushed away from the side wall of the conductive part 3 by the repulsive force between the conductive part 3 and the detection liquid 7 , and enter the detection path 6 . Nucleic acid amplification reaction. The present invention can avoid the problem that the detection liquid 7 is adsorbed on the side wall of the conductive part 3 and cannot enter the detection path, thus the subsequent nucleic acid amplification reaction cannot be realized by the arrangement of the conductive part 3 and the first driving electrode 4 .

Referring to FIG. 11 , the present invention further provides a nucleic acid detection cartridge 200 including the above-mentioned detection chip 100 . The nucleic acid detection cartridge 200 includes a cartridge body 201 and a connector 202 . The detection chip 100 is disposed in the box body 201 , and the connector 202 is electrically connected to the first driving electrode 4 and the driving element 9 in the detection chip 100 , respectively.

Please refer to FIG. 12, the present invention also provides a nucleic acid detection device 300, the nucleic acid detection device 300 includes a host 301 and the nucleic acid detection box 200 as described above, the host 301 is provided with a detection box installation slot 302, the nucleic acid detection box 200 is installed in the detection box installation slot 302 .

Compared with the prior art, the detection chip provided by the present invention has a simple structure design and is easy to assemble. By means of the design of the conductive part and the first driving electrode, the detection liquid adsorbed on the side wall of the conductive part can be returned to the detection path, thereby ensuring The normal progress of the nucleic acid amplification reaction.

100: Detection chip 1: The first cover 11: The first groove 2: Second cover 21: Second groove 22: Sample tank 3: Conductive part 31: First Surface 32: Second Surface 33: Raised part 34: First bump 35: Second bump 36: Third groove 37: Conductive part body 38: The first conductive layer 4,8: The first drive electrode 5: Channel 6: Detection path 7: Detection solution 9: Drive components 91: Drive circuit 911: Second drive electrode 912: Control Electrode 92: first dielectric layer 95: Opening 93: the second conductive layer 94: Second Dielectric Layer A: Sample adding area B: Reagent storage area C: nucleic acid amplification region D: Outlet area 200: Nucleic acid detection kit 201: Box 202: Connector 300: Nucleic acid detection equipment 301: host 302: Detection box installation slot

FIG. 1 is a schematic structural diagram of a detection chip provided by an embodiment of the present invention.

FIG. 2 is a schematic cross-sectional structure diagram of a detection chip according to an embodiment of the present invention.

FIG. 3 is a schematic structural diagram of a driving circuit in a detection chip according to an embodiment of the present invention.

FIG. 4 is a schematic cross-sectional structure diagram of a detection chip according to a second embodiment of the present invention.

FIG. 5 is a schematic cross-sectional structure diagram of a detection chip according to a third embodiment of the present invention.

FIG. 6 is a schematic cross-sectional structure diagram of a detection chip according to a fourth embodiment of the present invention.

FIG. 7 is a schematic cross-sectional structural diagram of a detection chip according to a fifth embodiment of the present invention.

FIG. 8 is a schematic cross-sectional structure diagram of a detection chip according to a sixth embodiment of the present invention.

FIG. 9 is a schematic structural diagram of a detection chip adsorbed on a conductive part in a detection chip according to an embodiment of the present invention.

FIG. 10 is a schematic structural diagram of the detection chip being driven away from the conductive portion in the detection chip according to an embodiment of the present invention.

FIG. 11 is a schematic structural diagram of a nucleic acid detection cassette provided by an embodiment of the present invention.

FIG. 12 is a schematic structural diagram of a nucleic acid detection device provided by an embodiment of the present invention.

100: Detection chip

1: The first cover

2: Second cover

22: Sample tank

3: Conductive part

Claims (10)

A detection chip, comprising: the first cover; the second cover; The conductive part includes a first surface and a second surface arranged opposite to each other, the first surface and the second surface are respectively adjacent to the first cover plate and the second cover plate, the first cover plate, The conductive part and the second cover plate are surrounded to form a channel, the channel includes a detection path, the channel is used to carry a detection liquid, and the detection liquid is charged; and two first driving electrodes, both of which are electrically connected to the conductive portion, and are used to energize or de-energize the conductive portion; Wherein, a driving force is generated between the conductive part and the detection liquid after electrification, and the driving force is used to drive the detection liquid to move in a direction away from the conductive part, so that the detection liquid moves to the desired position. on the detection path. The detection chip according to claim 1, wherein the first driving electrode is disposed between the first surface and the first cover plate or the second cover plate, and the first driving electrode is connected to the The first surface is electrically connected. The detection chip according to claim 1, wherein the first cover plate is provided with two first grooves corresponding to the first surface, and each of the first grooves is provided with one of the first grooves. For the driving electrode, the first surfaces of the two first grooves are protruded toward the direction away from the second surface to form two first bumps, and each of the first bumps is accommodated in a corresponding one of the first grooves, and the first bump is electrically connected to the first driving electrode. The detection chip according to claim 1, wherein the first cover plate is provided with a first groove corresponding to the first surface, and the second cover plate is provided with a second groove corresponding to the second surface a groove, and a first drive electrode is arranged in both the first groove and the second groove; The first surface protrudes toward the direction away from the second surface to form a first bump, the first bump is accommodated in the first groove, and the first bump is connected to a the first driving electrode is electrically connected; The second surface protrudes toward the direction away from the first surface to form a second bump, the second bump is accommodated in the second groove, and the second bump and the other The first driving electrodes are electrically connected. The detection chip according to claim 1, wherein the conductive portion is provided with two third grooves, and the two first driving electrodes are respectively disposed in the two third grooves and are both connected to the two third grooves. The conductive part is electrically connected. The detection chip according to claim 1, wherein the conductive part comprises a conductive part body and a first conductive layer disposed on a surface of the conductive part body on one side of the body close to the channel, the first conductive layer and the two Each of the first driving electrodes is electrically connected. The detection chip according to claim 1, further comprising a driving element, the driving element comprising a driving circuit arranged on a surface of the first cover plate close to the second cover plate, a driving circuit arranged on the first cover plate The second conductive layer on the side of the two cover plates close to the first cover plate, the first dielectric layer disposed on the side of the driving circuit close to the second cover plate, and the second conductive layer disposed on the side of the second cover plate. A second dielectric layer on one side of the first cover plate, the channel is formed between the first dielectric layer and the second dielectric layer, and the detection path is formed by the driving circuit. The detection chip according to claim 7, wherein the driving circuit comprises a plurality of second driving electrodes arranged in an array and control electrodes electrically connected to all the second driving electrodes, the second driving electrodes Connecting or disconnecting electricity with the second conductive layer is used to drive the detection liquid to move between two adjacent second driving electrodes. A nucleic acid detection box, comprising: box body; A detection chip, disposed in the box body, the detection chip is the detection chip according to any one of claim 1-8; and a connector, which is electrically connected to the driving element and the first driving electrode respectively; Wherein, the detection chip is used for performing nucleic acid amplification reaction on the detection solution to obtain nucleic acid amplification products. A nucleic acid detection device, comprising: host; The detection box installation slot is provided on the host, and the detection box installation slot is used to detachably install the nucleic acid detection box, and the nucleic acid detection box is the nucleic acid detection box according to claim 9.

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