SU417468A1 - - Google Patents

Description

one

The invention relates to the microbiological method of obtaining chemicals.

There is a known method of obtaining picolinic acid by chemical oxidation of 2-methylpyridine.

The method of producing picolinic acid does not require expensive oxidants, does not require the strict conditions of the oxy-peni process, and greatly simplifies the final product.

This is achieved by the fact that the oxidation is carried out with the help of microorganisms of the genus Nocardia. In STOiM kz of the genus Nocardia, strain 1A is used.

Shtam, m species 1A are grown on a medium containing a source of carbon (n-alkanes), nitrogen and mineral salts. The process takes place at atmospheric pressure, a temperature of 28-30 ° C without Obrazo Baths by-product.

Cultural features. On common nutrient media (co-peptone agar, wort agar, mineral medium with glucose), the colonies are pink-ocher in color, smooth and glossy, convex.

Morphological signs. On a mi-peptone broth in a 24-hour culture, the cell size is 0.15-1X8-Yumk.

Rod-shaped cells with irregular contours, angular shapes are pronounced, branching is rare, but distinct. For 2-3 days, the culture decays into coccoid fragments with a diameter of 0.5-1.0 microns. Physiological and biochemical characteristics. Is growing

at 15-37 ° C, mainly. at 28 ° C, pH 5-9. It has active catalase activity. Tween 40 hydrolyzes. Gelatin does not dilute. Starch, fiber, casein does not hydrolyze. Nitrates reduces.

The following carbohydrates assimilate without acid formation: glucose, galactose, arabinose xylose, sucrose, maltose, trehalose, lactose, raffinose, mannitol, sorbitol. Assimilates glycerin, ethanol, acetag,

propio.nat, butyrate, succinate, nitrate, and-al (cans: from 1nonane to gentadecane, olefin, docecen1, isoparaffin-squalane.

It does not assimilate palmitate, beisoate, salicylate, nicotinate, phenol, pyrocatechin, resorcinol, pafgalin, methylpyridines.

Relation to the source of nitrogen. Absorbs nitrites, nitrates, ammonium CO.IH urea, asnaragin, petoptop, casein hydrolyzate.

The culture of Nocardia species is grown on agar. Izovan1nsyy medium Erosh-ipa with hexadecane as a carbon source for two days. The suspension of the grown culture is sown in 250 ml Zlenmeyer flasks with liquid

medium Eroshipa spilled but 50 ml.

The composition of the medium Eroshina, g / l: MgSOi-THaO0,8

NaCl0.5

KHjPOi0.7

sterilized separately in distilled water

(NH4) 2HPO41.5

Water is added 1 L to the liter and hexadecane in the amount of 10 g / l.

2-methylyoolndin is added and each flask in the amount of 0.2 g. The flasks are incubated on a rocking chair at 30 ° C and 200 vol / M1IN. The transformation process is followed by chromatography. Picolinic acid 01 is formed on the second day. The oxidation is stopped on the fourth day, the culture liquid is evaporated to dryness on a rotary evaporator at 55 ° C, the precipitate is treated with hot benzene or carbon tetrachloride to extract picodonic acid, and the solvent is evaporated to dryness.

T. pl. Picolipic acid 135-137 ° C, yield 85%.

Subject invention

Claims (2)

1. A method for producing picolic acid by oxidation of 2-methyllyridine, characterized in that, in order to simplify the ethodess, the oxidation is carried out using microorhizisms of the genus Nocardia. 2. The method according to claim 1, from LI ch and y y and y with the fact that from the genus Nocardia use the chtamm species 1A.