SU1463755A1 - Method of producing hydrodrolysis media for growing fodder yeast - Google Patents

Abstract

The invention relates to the microbiological process, namely to methods for preparing hydrolytic media for growing feed stock. The purpose of the invention is to increase the yield of yeast. The hydrolyzate containing 4–6% of reducing substances (PB) is neutralized, nutrient salts are added, cooled, the precipitated sediment is separated and reacted with yeast cultures with glycolytic activity to reduce the PB content by 5–15% from the initial one. The method allows to increase the yeast yield by 4-18%. h

Description

one .

This invention relates to the microbiological industry, and specifically to a method for preparing hydrolytic media for leveling fodder yeast.

The purpose of the invention is the elevation and, 1, of the yeast.

The method is carried out as follows.

Neutralized with lime milk and ammonia water to pH 4.4, the hydrolyzate containing reducing substances 4-6% is enriched with nutrient salts, cooled, cleaned of suspended solids by means of sludge or filtration and fed to the reactor, where the resulting nutrient medium is reacted with yeast cultures with glycolytic activity to reduce the content of pe-. OUTPATURING substances (RB) for 5-15% of the original. Yeast can be used in both free and immobilized conditions. When exposed to yeast with glycolytic activity, furfural in the hydrolyzate, which is an inhibitor of yeast growth, is transformed into furfuryl alcohol in hydrolyzate. In addition, ethyl alcohol is formed, an additional source of carbon and in non-concentration concentrations of yeast growth. These processes lead to an increase in the yield of fodder yeast on hydrolysis media. When -.the decrease in the concentration of PB in the medium is less than 5%, there is no half of 4 Od CO H SL SP

 the transformation of furfural into furfuryl alcohol, which reduces the yield of feed drozukey, and a decrease in the concentration of PB 1% by more than 15% does not lead to a further increase in the biomass yield,

Example 1 (control). Guide- 7olizat preovesiny, containing,%: PB 4.3; furfural 0.0333; volatile acids 0.27, neutralized in two stages: lime with milk and ammonia to pH 4.4. Nutrient salts are added to neutralizer, ammonium sulfate 9.7; superphosphate 7.8; potassium chloride}, 3. After cooling, the medium is aerated for 1 h and filtered. On the substrate prepared in this way, an association of fodder yeast is grown; Hansenula anomala KIR5, Candida scottii KS-2, Candida tropicalls Per 5..

The yeast is grown in a laboratory fermenter with a working volume of 1 l with vigorous stirring and dilution rates among 25 h. The cultivation temperature is -38 ° C; pH 4.4 The residual content of the PB in the post-yeast mash is 0.16%.

The output is absolutely dry yeast (ASD) 52% of the RV.

PRI mme R 2. The hydrolyzate of wood containing,%: PB 4.3; furfuro 0.0333; volatile acids -0.27, neutralized stepwise to pH 4.5. Nutrient salts, ammonium sulfate 9.7, superphosphate 7.8; potassium chloride 1.3. After cooling, the medium is fed: Into a 40 cm flow reactor of column type with Candida tropicalis 649 yeast immobilized on apyomoborosilicate glass cases. The flow rate of the medium to the reactor is adjusted so that the outlet concentration of the RV is 4.08%, i.e. Content: The content of PB decreased in hydrolyzate by 5%. The concentration of furfuryl alcohol is 0.035%, ethyl 0.12%. Furfural is absent. After the reactor, the nutrient medium is fed to the fermenter, where fodder yeast is grown according to Example 1. The yield of the SDA is 58.8% of the PB. The residual concentration of PB is 0.18%.

Example 3. The method is carried out according to Example 2, but the feed rate of the hydrolyzate to the reactor is adjusted so that the contents

0

five

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RV output decreased by 10%. The RV content in the resulting medium is 3.87%, furfuryl alcohol is 0.034, and ethyl alcohol is O, 19%. The output of the SDA is 61.5% of the RV. The residual concentration of PB is 0.16%.

Ir im p 4. The method is carried out according to Example 2, but the feed rate of the hydrolyzate to the reactor is adjusted so that the output PB content is reduced by 15%. The content of RV in the resulting medium is 3.67%, furfuryl alcohol is 0.035%, and ethyl alcohol is 0.31%. The yield of ASD is 59.2% of PB, the residual concentration of PB is 0.22%.

Example 5 The method is carried out according to Example 2, but the decrease in the RV content is carried out using free cells of the Candida tropicalis 649 yeast, which are cultured under periodic conditions until the RV content is reduced by 7%. The RV content in the resulting medium is 4.0%, furfuryl alcohol 25 is 0.034%, ethyl alcohol is 0.15%. Yeast biomass is separated; sparacine; the resulting medium is grown & 1 1 ASD APP 60.1% of

thirty

her as well

feed yeast,

PB

Example 6. The method is carried out according to Example 2, but using cells immobilized in a foam of a gas-liquid interface. The feed rate of the medium into the reactor is regulated so that the content of the RV is reduced by 8%. The concentration of furfuryl alcohol is 0.035%, ethyl 0.17%. The output of the ASD of 59.7%. from PB.

40 Ir and mep 7. The method is carried out according to Example 2, but using a reactor with immobilized Saccharomyces cerevisiae AB-1 cells. The concentration of furfuryl alcohol in 45 hydrolyzate after the reactor was 0.24%, ethyl 0.08%. The output of the ASD 57,0% of RV.

II p. And measure 8. The method is carried out according to Example 2, to which Hansenula-anonala KIR5 and Trichosporon cutaneun | Tabg-4 are used as feed yeast. The yield of ASD is increased by 4.2% compared with the control.

Thus, the proposed method of preparing hydrolytic media for growing fodder No. 1x yeast makes it possible to process concentrated media and increase the yield

514637556

biomass at 4-18% compared with the sediment, adding to the medium piizvestnym. good salt, about tl to tea g

Claims (1)

U and with the fact that, for the purpose of a higher formula of the invention, no yeast yield, after separation sediment medium is subjected to interaction with the method of preparation of hydrolysis with yeast cultures, possessing glycolytic activity for strengthening fodder yeast, providing for the neutralization of the ere-d ° reduction of the content of reducing dy, cooling, separation of substances formed by fo 5-15% from the original.