SU1293613A1 - Method and apparatus for concentrating substances in liquid - Google Patents

Abstract

The invention relates to the preparative separation and concentration of biological substances and polyions in mixtures by electrophoresis and can be used in microbiology, chemistry and scientific research. The purpose of the invention is to accelerate the separation process, increase the degree of separation, eliminate overheating of the mixture to be separated. This is achieved by the fact that the container for the mixture to be divided is made in the form of a cone made of cellophane with a hole at the apex of a cone placed in a conical cavity cut in a gel block. In this case, the number of electrodes is three, the first of them is placed on the side of the cone end, and the other two, which have a charge sign opposite to the first electrode, are located on both sides of the storage tank. Both of these electrodes are alternately included in the electrical circuit of the device. 1 il. (L e SA :) O) her

Description

The invention approaches the technique of preparative separation of 1substances in liquids, in particular, the concentration of polyions in microbiological liquids.

The purpose of the invention is to accelerate the process, increase the degree of concentration of substances, decrease the degree of adsorption of polyions of dialysis films,

The drawing shows a diagram of the device that implements pred.lah. Hagoy way.

The device contains a container of 1 lp of a buffer solution, qi: indric block 2, of sweat and acrylamide electrically conductive gel, in which a conical capacitance is cut, covered with D1 aliz: 0, - film for a test of a splittable lcd, camera-tip 3 s - dialysis films 4 and othioliptsi; - with a choke pipe 5, a buffer tank (s, coupled to end capacitances 7, the electric field is determined by electrode 8, located in tank 1, and electrodes 9 and 10, located in end capacitances 7, n 5 and the electrodes 10 and 11 are equipped with a device (hp shown) x pervoocheredno1 of connecting to a current source.

The device is working afterwards (i6).

When the containers are filled with 1, 2, 6 and 7 and the installation of electrodes 9, 10 and 8 ko; .centric substances, for example, polyions, occurs as follows: polyions move in an electric field from the ground, affective electrode ,;

8 to the positive electrode 9 or 10 under the conditions of the absence of a high density of electrical, 1-: blown like a buffer solution bsmt: w o g o s e c e n, h, and l o r a b o h G m u r a s t thief and cylinder 2. 11о.) L1LONY ,, HAVED

into the camera-drive 3, coBepiuaioT oscillatory in a single-sided | lp dialysis film 4 s (ksvetvestvenno about alternate boat1: more common than the first type

9 or 10 to the positive pole of the lic current source. Concentrated in liquid storage chambers 3 polyion is retracted by means of a shut-off pipe 5 after either a certain time has passed, or in parts with monitoring of the concentration of polyions to determine the end of the concentration process.

536132

When concentrating substances, the adsorption of E is still reduced to the test on dialysis films, the leverage of the solution decreases, and the number of 5 ions is less destroyed. Concentration of the enzyme x-amylase from the volume of 0–5 l.ConmKg- centrifuging of the novedate to 5000 was carried out.

ml

jj- (without loss of activity of the enzyme. The center of concentration is 50 times higher. when concentrated in a known manner and device,

Ф о рм ул and

15

about and about and and

1. A method of concentrating substances and a liquid passing an electric current along C) SI colus oi; -; - contact with liquid; with a capacitance and storage chamber and removing a part of liquid through / l, thin films, collecting enriched - ac; fluids, fluids, and tu - and - so that, in order to accelerate the proliferation, increase the stress and concentration (4) and reduce the adsorption of lienjecTB on the surface of dialysis membranes; the electric lol in the accumulator l-lp camera changes the orthogonal direction of the nol l cone of the 1st capacitance and is periodically changed to the opposite, and the dialysis films are placed orthographically in the accumulator chamber,

2. Device for drying

(lusoba by nl, sol.bald com); h: ny (capacitance for a liquid with a concentration of P / e in another sam and, n a n on the 11th chamber and chambers for bu (a laser mixture of electrodes separated from a kamsp laser) and a conical capacitor;; type-; 1 liznyi l. klpchami, a tlilu and a - C e s T., which is cone e; - -5kkost 1) is located on the Tsiligli-il'skaya; olonke from electrolytic Shdny ge.ch and BLJ-l ;; whose axis is perp There is an endic axis of the cone capacity, on the side of the base; a cone is located a chamber for the bu (|) mixture with the cathode, and

.

At least two of them are located in the chambers for the buffer mixture symmetrically with the axis of the conical tank.

Editor A. Revin

Compiled by L. Zharkova

Tehred V. Kadar Proofreader L. Pilipenko

379/48 Circulation 777

VNIIPI State Committee LLLF

for inventions and discoveries 113035, Moscow, Zh-35, Raushsk nab., 4/5

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Production

printing enterprise, Uzhgorod, st. Project, 4

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Claims (2)

1. A method of concentrating substances in a liquid by passing an electric current along the axis of a conical container with liquid and accumulating chamber and removing part of the liquid through / dialysis films, collecting the enriched part of the liquid? The reason is that, in order to accelerate the process of increasing the efficiency of concentration and decreasing the adsorption of substances on and on the surface of the dialysis films _? the direction of the electric field in the storage chamber is changed i and the orthogonal base is located in July and the ol in the conical container and periodically reversed, and the dialysis films are arranged orthogonally to the field direction in the storage chamber, 2 "A device for implementing the method according to p." 1, containing a conical container for liquid with concent. substances, the storage chamber and chambers for the buffer mixture and electrodes., separated from the storage chamber and the conical container by lavision films, with the exception that the conical container is extended Is it made of electrically conductive gel and made of electrically conductive semi-permeable material when wet, is the top of the cone installed in the middle of the tube-shaped storage chamber? whose axis is perpendicular to the axis of the conical tank? on the side of the base of the cone is a chamber for the buffer mixture with the cathode, and the anodes? the number of which is not less than two, are located in the chambers for the buffer mixture symmetrically to the axis of the conical capacity.