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SG11202000966XA - Integration sites in CHO cells - Google Patents

Integration sites in CHO cells

Info

Publication number
SG11202000966XA
SG11202000966XA SG11202000966XA SG11202000966XA SG11202000966XA SG 11202000966X A SG11202000966X A SG 11202000966XA SG 11202000966X A SG11202000966X A SG 11202000966XA SG 11202000966X A SG11202000966X A SG 11202000966XA SG 11202000966X A SG11202000966X A SG 11202000966XA
Authority
SG
Singapore
Prior art keywords
cho cells
integration sites
sites
integration
cho
Prior art date
Application number
SG11202000966XA
Inventor
Markus Mueller
Jochen Schaub
Christian Bernloehr
Jennifer Koenitzer
Original Assignee
Boehringer Ingelheim Int
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Boehringer Ingelheim Int filed Critical Boehringer Ingelheim Int
Publication of SG11202000966XA publication Critical patent/SG11202000966XA/en

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06Animal cells or tissues; Human cells or tissues
    • C12N5/0602Vertebrate cells
    • C12N5/0681Cells of the genital tract; Non-germinal cells from gonads
    • C12N5/0682Cells of the female genital tract, e.g. endometrium; Non-germinal cells from ovaries, e.g. ovarian follicle cells
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/87Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90Stable introduction of foreign DNA into chromosome
    • C12N15/902Stable introduction of foreign DNA into chromosome using homologous recombination
    • C12N15/907Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K16/00Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/16Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22Ribonucleases RNAses, DNAses
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2317/00Immunoglobulins specific features
    • C07K2317/10Immunoglobulins specific features characterized by their source of isolation or production
    • C07K2317/14Specific host cells or culture conditions, e.g. components, pH or temperature
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2319/00Fusion polypeptide
    • C07K2319/80Fusion polypeptide containing a DNA binding domain, e.g. Lacl or Tet-repressor
    • C07K2319/81Fusion polypeptide containing a DNA binding domain, e.g. Lacl or Tet-repressor containing a Zn-finger domain for DNA binding
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00Structure or type of the nucleic acid
    • C12N2310/10Type of nucleic acid
    • C12N2310/20Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPRs]
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2510/00Genetically modified cells
    • C12N2510/02Cells for production
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00Nucleic acids vectors
    • C12N2800/30Vector systems comprising sequences for excision in presence of a recombinase, e.g. loxP or FRT

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Cell Biology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Mycology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Immunology (AREA)
  • Reproductive Health (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
SG11202000966XA 2017-08-11 2018-08-10 Integration sites in CHO cells SG11202000966XA (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP17185988 2017-08-11
PCT/EP2018/071733 WO2019030373A1 (en) 2017-08-11 2018-08-10 Integration sites in CHO cells

Publications (1)

Publication Number Publication Date
SG11202000966XA true SG11202000966XA (en) 2020-02-27

Family

ID=59686752

Family Applications (1)

Application Number Title Priority Date Filing Date
SG11202000966XA SG11202000966XA (en) 2017-08-11 2018-08-10 Integration sites in CHO cells

Country Status (8)

Country Link
US (2) US11560549B2 (en)
EP (1) EP3665291B1 (en)
JP (1) JP7087061B2 (en)
KR (1) KR102531749B1 (en)
CN (1) CN110997925A (en)
CA (1) CA3068779A1 (en)
SG (1) SG11202000966XA (en)
WO (1) WO2019030373A1 (en)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2020252840A1 (en) * 2019-04-02 2021-11-04 Chugai Seiyaku Kabushiki Kaisha Method of introducing target-specific foreign gene
JP7472167B2 (en) * 2019-04-18 2024-04-22 シグマ-アルドリッチ・カンパニー・リミテッド・ライアビリティ・カンパニー Stable targeted integration
EP3901266A1 (en) * 2020-04-22 2021-10-27 LEK Pharmaceuticals d.d. Super-enhancers for recombinant gene expression in cho cells
US20220356501A1 (en) * 2021-05-10 2022-11-10 Nikhil Goel Engineered cells for increased production of difficult-to-express proteins
CN113355360A (en) * 2021-05-28 2021-09-07 上海碧博生物医药科技有限公司 Construction method of GS gene knockout CHO-K1 cell strain and suspension cell monoclonality
CN114085841B (en) * 2021-11-23 2022-07-15 江南大学 Site for stably expressing protein in CHO cell gene NW _003614092.1 and application thereof
CN114058625B (en) * 2021-11-25 2022-07-15 江南大学 Site for stably expressing protein in CHO cell gene NW _003613781.1 and application thereof
CN117305334B (en) * 2022-06-21 2024-11-08 深圳太力生物技术有限责任公司 Targeted integration cell, preparation method thereof and method for producing target gene expression product
CN118726475A (en) * 2023-03-28 2024-10-01 深圳太力生物技术有限责任公司 Nucleic acid, recombinant vector, targeted integration cell, gene expression method and application

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP3150340B2 (en) 1990-11-13 2001-03-26 イムネクス コーポレイション Bifunctional selectable fusion gene
EP0804590A1 (en) 1993-05-21 1997-11-05 Targeted Genetics Corporation Bifunctional selectable fusion genes based on the cytosine deaminase (cd) gene
US6521419B1 (en) * 1998-09-22 2003-02-18 Kanakaraju Koduri Expression vectors containing hot spot for increased recombinant protein expression in transfected cells
US7422889B2 (en) 2004-10-29 2008-09-09 Stowers Institute For Medical Research Dre recombinase and recombinase systems employing Dre recombinase
AR069511A1 (en) * 2007-11-30 2010-01-27 Boehringer Ingelheim Pharma ATT RECOMBINATION SEQUENCES THAT ALLOW THE GENERATION OF AN IMPROVED PROTEIN EXPRESSION SYSTEM
AU2014281472A1 (en) * 2013-06-19 2016-01-21 Sigma-Aldrich Co. Llc. Targeted integration
CN107109434A (en) * 2014-10-23 2017-08-29 瑞泽恩制药公司 Novel CHO integration sites and its purposes

Also Published As

Publication number Publication date
US20230131164A1 (en) 2023-04-27
US20200216815A1 (en) 2020-07-09
EP3665291A1 (en) 2020-06-17
KR20200038462A (en) 2020-04-13
US11560549B2 (en) 2023-01-24
AU2018315428A1 (en) 2020-01-16
CN110997925A (en) 2020-04-10
US12018285B2 (en) 2024-06-25
JP2020530998A (en) 2020-11-05
EP3665291B1 (en) 2021-10-06
JP7087061B2 (en) 2022-06-20
CA3068779A1 (en) 2019-02-14
KR102531749B1 (en) 2023-05-10
WO2019030373A1 (en) 2019-02-14

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