SG11202000966XA - Integration sites in CHO cells - Google Patents
Integration sites in CHO cellsInfo
- Publication number
- SG11202000966XA SG11202000966XA SG11202000966XA SG11202000966XA SG11202000966XA SG 11202000966X A SG11202000966X A SG 11202000966XA SG 11202000966X A SG11202000966X A SG 11202000966XA SG 11202000966X A SG11202000966X A SG 11202000966XA SG 11202000966X A SG11202000966X A SG 11202000966XA
- Authority
- SG
- Singapore
- Prior art keywords
- cho cells
- integration sites
- sites
- integration
- cho
- Prior art date
Links
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0681—Cells of the genital tract; Non-germinal cells from gonads
- C12N5/0682—Cells of the female genital tract, e.g. endometrium; Non-germinal cells from ovaries, e.g. ovarian follicle cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
- C12N15/90—Stable introduction of foreign DNA into chromosome
- C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
- C12N15/907—Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
- C12N9/22—Ribonucleases RNAses, DNAses
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2317/00—Immunoglobulins specific features
- C07K2317/10—Immunoglobulins specific features characterized by their source of isolation or production
- C07K2317/14—Specific host cells or culture conditions, e.g. components, pH or temperature
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/80—Fusion polypeptide containing a DNA binding domain, e.g. Lacl or Tet-repressor
- C07K2319/81—Fusion polypeptide containing a DNA binding domain, e.g. Lacl or Tet-repressor containing a Zn-finger domain for DNA binding
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2310/00—Structure or type of the nucleic acid
- C12N2310/10—Type of nucleic acid
- C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPRs]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2510/00—Genetically modified cells
- C12N2510/02—Cells for production
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2800/00—Nucleic acids vectors
- C12N2800/30—Vector systems comprising sequences for excision in presence of a recombinase, e.g. loxP or FRT
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Biophysics (AREA)
- Cell Biology (AREA)
- Plant Pathology (AREA)
- Physics & Mathematics (AREA)
- Medicinal Chemistry (AREA)
- Mycology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Immunology (AREA)
- Reproductive Health (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
EP17185988 | 2017-08-11 | ||
PCT/EP2018/071733 WO2019030373A1 (en) | 2017-08-11 | 2018-08-10 | Integration sites in CHO cells |
Publications (1)
Publication Number | Publication Date |
---|---|
SG11202000966XA true SG11202000966XA (en) | 2020-02-27 |
Family
ID=59686752
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
SG11202000966XA SG11202000966XA (en) | 2017-08-11 | 2018-08-10 | Integration sites in CHO cells |
Country Status (8)
Country | Link |
---|---|
US (2) | US11560549B2 (en) |
EP (1) | EP3665291B1 (en) |
JP (1) | JP7087061B2 (en) |
KR (1) | KR102531749B1 (en) |
CN (1) | CN110997925A (en) |
CA (1) | CA3068779A1 (en) |
SG (1) | SG11202000966XA (en) |
WO (1) | WO2019030373A1 (en) |
Families Citing this family (9)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
AU2020252840A1 (en) * | 2019-04-02 | 2021-11-04 | Chugai Seiyaku Kabushiki Kaisha | Method of introducing target-specific foreign gene |
JP7472167B2 (en) * | 2019-04-18 | 2024-04-22 | シグマ-アルドリッチ・カンパニー・リミテッド・ライアビリティ・カンパニー | Stable targeted integration |
EP3901266A1 (en) * | 2020-04-22 | 2021-10-27 | LEK Pharmaceuticals d.d. | Super-enhancers for recombinant gene expression in cho cells |
US20220356501A1 (en) * | 2021-05-10 | 2022-11-10 | Nikhil Goel | Engineered cells for increased production of difficult-to-express proteins |
CN113355360A (en) * | 2021-05-28 | 2021-09-07 | 上海碧博生物医药科技有限公司 | Construction method of GS gene knockout CHO-K1 cell strain and suspension cell monoclonality |
CN114085841B (en) * | 2021-11-23 | 2022-07-15 | 江南大学 | Site for stably expressing protein in CHO cell gene NW _003614092.1 and application thereof |
CN114058625B (en) * | 2021-11-25 | 2022-07-15 | 江南大学 | Site for stably expressing protein in CHO cell gene NW _003613781.1 and application thereof |
CN117305334B (en) * | 2022-06-21 | 2024-11-08 | 深圳太力生物技术有限责任公司 | Targeted integration cell, preparation method thereof and method for producing target gene expression product |
CN118726475A (en) * | 2023-03-28 | 2024-10-01 | 深圳太力生物技术有限责任公司 | Nucleic acid, recombinant vector, targeted integration cell, gene expression method and application |
Family Cites Families (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP3150340B2 (en) | 1990-11-13 | 2001-03-26 | イムネクス コーポレイション | Bifunctional selectable fusion gene |
EP0804590A1 (en) | 1993-05-21 | 1997-11-05 | Targeted Genetics Corporation | Bifunctional selectable fusion genes based on the cytosine deaminase (cd) gene |
US6521419B1 (en) * | 1998-09-22 | 2003-02-18 | Kanakaraju Koduri | Expression vectors containing hot spot for increased recombinant protein expression in transfected cells |
US7422889B2 (en) | 2004-10-29 | 2008-09-09 | Stowers Institute For Medical Research | Dre recombinase and recombinase systems employing Dre recombinase |
AR069511A1 (en) * | 2007-11-30 | 2010-01-27 | Boehringer Ingelheim Pharma | ATT RECOMBINATION SEQUENCES THAT ALLOW THE GENERATION OF AN IMPROVED PROTEIN EXPRESSION SYSTEM |
AU2014281472A1 (en) * | 2013-06-19 | 2016-01-21 | Sigma-Aldrich Co. Llc. | Targeted integration |
CN107109434A (en) * | 2014-10-23 | 2017-08-29 | 瑞泽恩制药公司 | Novel CHO integration sites and its purposes |
-
2018
- 2018-08-10 SG SG11202000966XA patent/SG11202000966XA/en unknown
- 2018-08-10 CN CN201880051636.5A patent/CN110997925A/en active Pending
- 2018-08-10 US US16/637,914 patent/US11560549B2/en active Active
- 2018-08-10 WO PCT/EP2018/071733 patent/WO2019030373A1/en unknown
- 2018-08-10 CA CA3068779A patent/CA3068779A1/en active Pending
- 2018-08-10 EP EP18749820.9A patent/EP3665291B1/en active Active
- 2018-08-10 KR KR1020207003299A patent/KR102531749B1/en active IP Right Grant
- 2018-08-10 JP JP2020506795A patent/JP7087061B2/en active Active
-
2022
- 2022-12-06 US US18/062,198 patent/US12018285B2/en active Active
Also Published As
Publication number | Publication date |
---|---|
US20230131164A1 (en) | 2023-04-27 |
US20200216815A1 (en) | 2020-07-09 |
EP3665291A1 (en) | 2020-06-17 |
KR20200038462A (en) | 2020-04-13 |
US11560549B2 (en) | 2023-01-24 |
AU2018315428A1 (en) | 2020-01-16 |
CN110997925A (en) | 2020-04-10 |
US12018285B2 (en) | 2024-06-25 |
JP2020530998A (en) | 2020-11-05 |
EP3665291B1 (en) | 2021-10-06 |
JP7087061B2 (en) | 2022-06-20 |
CA3068779A1 (en) | 2019-02-14 |
KR102531749B1 (en) | 2023-05-10 |
WO2019030373A1 (en) | 2019-02-14 |
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