SG11201900058YA - Method of generating mesenchymal stem cells and uses thereof - Google Patents
Method of generating mesenchymal stem cells and uses thereofInfo
- Publication number
- SG11201900058YA SG11201900058YA SG11201900058YA SG11201900058YA SG11201900058YA SG 11201900058Y A SG11201900058Y A SG 11201900058YA SG 11201900058Y A SG11201900058Y A SG 11201900058YA SG 11201900058Y A SG11201900058Y A SG 11201900058YA SG 11201900058Y A SG11201900058Y A SG 11201900058YA
- Authority
- SG
- Singapore
- Prior art keywords
- singapore
- international
- cells
- growth factor
- genome
- Prior art date
Links
- 210000002901 mesenchymal stem cell Anatomy 0.000 title abstract 6
- 238000000034 method Methods 0.000 title abstract 4
- 210000004027 cell Anatomy 0.000 abstract 5
- 210000003716 mesoderm Anatomy 0.000 abstract 4
- 102000018233 Fibroblast Growth Factor Human genes 0.000 abstract 3
- 108050007372 Fibroblast Growth Factor Proteins 0.000 abstract 3
- 229940126864 fibroblast growth factor Drugs 0.000 abstract 3
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 abstract 2
- 102000010780 Platelet-Derived Growth Factor Human genes 0.000 abstract 2
- 108010038512 Platelet-Derived Growth Factor Proteins 0.000 abstract 2
- 239000003795 chemical substances by application Substances 0.000 abstract 2
- 239000001963 growth medium Substances 0.000 abstract 2
- 210000001811 primitive streak Anatomy 0.000 abstract 2
- 108010059616 Activins Proteins 0.000 abstract 1
- 241000234282 Allium Species 0.000 abstract 1
- 235000002732 Allium cepa var. cepa Nutrition 0.000 abstract 1
- 102000007350 Bone Morphogenetic Proteins Human genes 0.000 abstract 1
- 108010007726 Bone Morphogenetic Proteins Proteins 0.000 abstract 1
- 208000020084 Bone disease Diseases 0.000 abstract 1
- 102100032912 CD44 antigen Human genes 0.000 abstract 1
- 102000010834 Extracellular Matrix Proteins Human genes 0.000 abstract 1
- 108010037362 Extracellular Matrix Proteins Proteins 0.000 abstract 1
- 102000016970 Follistatin Human genes 0.000 abstract 1
- 108010014612 Follistatin Proteins 0.000 abstract 1
- 101000868273 Homo sapiens CD44 antigen Proteins 0.000 abstract 1
- 102100026818 Inhibin beta E chain Human genes 0.000 abstract 1
- 102000001253 Protein Kinase Human genes 0.000 abstract 1
- 239000012190 activator Substances 0.000 abstract 1
- 239000000488 activin Substances 0.000 abstract 1
- 229960005070 ascorbic acid Drugs 0.000 abstract 1
- 235000010323 ascorbic acid Nutrition 0.000 abstract 1
- 239000011668 ascorbic acid Substances 0.000 abstract 1
- 210000004271 bone marrow stromal cell Anatomy 0.000 abstract 1
- 229940112869 bone morphogenetic protein Drugs 0.000 abstract 1
- 210000000845 cartilage Anatomy 0.000 abstract 1
- 208000015100 cartilage disease Diseases 0.000 abstract 1
- 239000006143 cell culture medium Substances 0.000 abstract 1
- 238000012136 culture method Methods 0.000 abstract 1
- 238000012258 culturing Methods 0.000 abstract 1
- 210000002744 extracellular matrix Anatomy 0.000 abstract 1
- 239000003112 inhibitor Substances 0.000 abstract 1
- 230000008520 organization Effects 0.000 abstract 1
- 210000001778 pluripotent stem cell Anatomy 0.000 abstract 1
- 108060006633 protein kinase Proteins 0.000 abstract 1
- 102220240796 rs553605556 Human genes 0.000 abstract 1
- 230000011664 signaling Effects 0.000 abstract 1
- VBEQCZHXXJYVRD-GACYYNSASA-N uroanthelone Chemical compound C([C@@H](C(=O)N[C@H](C(=O)N[C@@H](CS)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](CS)C(=O)N[C@H](C(=O)N[C@@H]([C@@H](C)CC)C(=O)NCC(=O)N[C@@H](CC=1C=CC(O)=CC=1)C(=O)N[C@@H](CO)C(=O)NCC(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CS)C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CC=1C2=CC=CC=C2NC=1)C(=O)N[C@@H](CC=1C2=CC=CC=C2NC=1)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCNC(N)=N)C(O)=O)C(C)C)[C@@H](C)O)NC(=O)[C@H](CO)NC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CO)NC(=O)[C@H](CCC(O)=O)NC(=O)[C@@H](NC(=O)[C@H](CC=1NC=NC=1)NC(=O)[C@H](CCSC)NC(=O)[C@H](CS)NC(=O)[C@@H](NC(=O)CNC(=O)CNC(=O)[C@H](CC(N)=O)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CS)NC(=O)[C@H](CC=1C=CC(O)=CC=1)NC(=O)CNC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CC=1C=CC(O)=CC=1)NC(=O)[C@H](CO)NC(=O)[C@H](CO)NC(=O)[C@H]1N(CCC1)C(=O)[C@H](CS)NC(=O)CNC(=O)[C@H]1N(CCC1)C(=O)[C@H](CC=1C=CC(O)=CC=1)NC(=O)[C@H](CO)NC(=O)[C@@H](N)CC(N)=O)C(C)C)[C@@H](C)CC)C1=CC=C(O)C=C1 VBEQCZHXXJYVRD-GACYYNSASA-N 0.000 abstract 1
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- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
- C12N5/0662—Stem cells
- C12N5/0663—Bone marrow mesenchymal stem cells (BM-MSC)
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- A61K35/28—Bone marrow; Haematopoietic stem cells; Mesenchymal stem cells of any origin, e.g. adipose-derived stem cells
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Abstract
Mesoderm FOXF+- Handl+ APLNR1 MSC CD14-, CD34-, CD45-, CO29+, CD44+, C049c+, 0373+, CD105+, CD151+, CD166+ (12) INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property Organization International Bureau (43) International Publication Date 11 January 2018 (11.01.2018) WIP0 I PCT onion OH oI0IIIIIIII1101HDIDMDlII111111 DIIIIII (10) International Publication Number WO 2018/009147 Al (51) International Patent Classification: C12N 5/0775 (2010.01) A61P 19/04 (2006.01) A61K 35/28 (2015.01) (21) International Application Number: PCT/SG2017/050339 (22) International Filing Date: 04 July 2017 (04.07.2017) (25) Filing Language: English (26) Publication Language: English (30) Priority Data: 10201605481T 04 July 2016 (04.07.2016) SG (71) Applicant: AGENCY FOR SCIENCE, TECHNOLO- GY AND RESEARCH [SG/SG]; 1 Fusionopolis Way, #20-10 Connexis North Tower, Singapore 138632 (SG). (72) Inventors: LIU, Tong Ming; c/o Genome Institute of Sin- gapore, 60 Biopolis Street, Genome Building, Singapore 138672 (SG). LIM, Bing; c/o Genome Institute of Sin- gapore, 60 Biopolis Street, Genome Building, Singapore 138672 (SG). LI, Pin; c/o Genome Institute of Singapore, 60 Biopolis Street, Genome Building, Singapore 138672 (SG). (74) Agent: SPRUSON & FERGUSON (ASIA) PTE LTD; P.O. Box 1531, Robinson Road Post Office, Singapore 903031 (SG). (81) Designated States (unless otherwise indicated, for every kind of national protection available): AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. (84) Designated States (unless otherwise indicated, for every kind of regional protection available): ARIPO (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, TJ, TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG). Declarations under Rule 4.17: — of inventorship (Rule 4.17(iv)) Published: — with international search report (Art. 21(3)) = (54) Title: METHOD OF GENERATING MESENCHYMAL STEM CELLS AND USES THEREOF 1-1 N 1-1 t:T O O GC O 1-1 N O ACF FBRFs FPEVc do di d2 Stage 1 d10 Stage 2 d21 Stage 3 (57) : The present disclosure provides a method of generating mature mesenchymal stem cells (MSC) from lateral plate mesoderm comprising culturing the lateral plate mesoderm cells on an extracellular matrix in a MSC cell culture media comprising (i) fibroblast growth factor; (ii) platelet-derived growth factor (PDGF); (iii) epidermal growth factor (EGF) and (iv) ascorbic acid. Also claimed are MSCs obtained by said culture method and a method of treating cartilage and bone disease in a patient using said cells. In addition, a culture medium for deriving primitive streak mesendoderm cells from pluripotent stem cells comprising (a) activin; (b) WNT- signalling activator; and/or (c) fibroblast growth factor and a culture medium for deriving lateral plate mesoderm cells from primitive streak mesendoderm cells comprising (1) fibroblast growth factor; (2) bone morphogenetic protein; (3) follistatin; and optionally Rho- associated protein kinase (ROCK) inhibitor are claimed.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| SG10201605481T | 2016-07-04 | ||
| PCT/SG2017/050339 WO2018009147A1 (en) | 2016-07-04 | 2017-07-04 | Method of generating mesenchymal stem cells and uses thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| SG11201900058YA true SG11201900058YA (en) | 2019-02-27 |
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| SG11201900058YA SG11201900058YA (en) | 2016-07-04 | 2017-07-04 | Method of generating mesenchymal stem cells and uses thereof |
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| US (1) | US11180733B2 (en) |
| SG (1) | SG11201900058YA (en) |
| WO (1) | WO2018009147A1 (en) |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| EP3921408A1 (en) * | 2019-02-08 | 2021-12-15 | University Of Southampton | Cartilage tissue |
| CN111019889A (en) * | 2019-12-25 | 2020-04-17 | 依科赛生物科技（太仓）有限公司 | Mesenchymal stem cell chemical component-limited serum-free culture medium and application thereof |
| CN111166884B (en) * | 2020-01-15 | 2021-12-03 | 广州中医药大学第一附属医院 | Application of Foxf1 gene in preparation of medicine for treating osteoporosis |
| FR3107709B1 (en) * | 2020-02-28 | 2023-09-15 | Ic Biosolutions | METHOD FOR PRODUCING MESENCHYMAL STEM CELLS COMPETENT FOR CARTILAGE REGENERATION FROM EQUINE PLURIPOTENT STEM CELLS |
| CN112870361A (en) * | 2021-01-28 | 2021-06-01 | 重庆医科大学 | Use of iron death inhibitor for preparing medicine for preventing or treating osteoporosis or bone loss caused by iron overload |
| US20240279608A1 (en) * | 2021-05-26 | 2024-08-22 | University Of Southern California | Production processes for highly viable, engraftable human chondrocytes from stem cells |
| CN114480266B (en) * | 2022-01-21 | 2024-07-26 | 中山大学附属第三医院 | Composition for improving homogeneity and reducing immunogenicity of mesenchymal stem cells |
| US20230257714A1 (en) * | 2022-02-07 | 2023-08-17 | Smartcella Solutions Ab | Methods and compositions for generating human induced mesenchymal stem cells |
| CN114672455B (en) * | 2022-03-25 | 2024-07-09 | 中山大学 | Method for inducing bone marrow stromal cells by utilizing pluripotent stem cells |
| CN114891737B (en) * | 2022-04-25 | 2023-05-12 | 中山大学 | Preparation method and application of multipotent stem cell-derived kidney mesenchymal stem cells |
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| JP2002529071A (en) * | 1998-11-09 | 2002-09-10 | コンソルツィオ・ペール・ラ・ジェスティオーネ・デル・チェントロ・ディ・ビオテクノロジー・アヴァンツァテ | Serum-free medium for chondrocyte-like cells |
| CN101641436A (en) * | 2007-01-30 | 2010-02-03 | 佐治亚大学研究基金会 | Be used to produce the promptly stable mesendoderm cell mass of early stage mesoblastema of entoderm and mesoblastema system and multipotency wandering cell (MMC) |
| US20110236971A2 (en) | 2007-09-25 | 2011-09-29 | Maksym Vodyanyk | Generation of Clonal Mesenchymal Progenitors and Mesenchymal Stem Cell Lines Under Serum-Free Conditions |
| JP5843775B2 (en) | 2009-10-13 | 2016-01-13 | ステムセル テクノロジーズ インコーポレーティッド | Manipulation of osmolality to differentiate stem cells |
| CA2843050A1 (en) | 2010-07-26 | 2012-02-02 | The University Of Manchester | Targeted differentiation of stem cells |
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2017
- 2017-07-04 US US16/315,624 patent/US11180733B2/en active Active
- 2017-07-04 SG SG11201900058YA patent/SG11201900058YA/en unknown
- 2017-07-04 WO PCT/SG2017/050339 patent/WO2018009147A1/en active Application Filing
Also Published As
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|---|---|
| US20190322987A1 (en) | 2019-10-24 |
| WO2018009147A1 (en) | 2018-01-11 |
| US11180733B2 (en) | 2021-11-23 |
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