SG11201811021YA - Method to screen for a mutant within a population of organisms by applying a pooling and splitting approach - Google Patents
Method to screen for a mutant within a population of organisms by applying a pooling and splitting approachInfo
- Publication number
- SG11201811021YA SG11201811021YA SG11201811021YA SG11201811021YA SG11201811021YA SG 11201811021Y A SG11201811021Y A SG 11201811021YA SG 11201811021Y A SG11201811021Y A SG 11201811021YA SG 11201811021Y A SG11201811021Y A SG 11201811021YA SG 11201811021Y A SG11201811021Y A SG 11201811021YA
- Authority
- SG
- Singapore
- Prior art keywords
- organisms
- international
- copenhagen
- mutant
- vej
- Prior art date
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
- C12Q1/6895—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for plants, fungi or algae
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/686—Polymerase chain reaction [PCR]
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/01—Preparation of mutants without inserting foreign genetic material therein; Screening processes therefor
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/005—Methods for micropropagation; Vegetative plant propagation using cell or tissue culture techniques
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H6/00—Angiosperms, i.e. flowering plants, characterised by their botanic taxonomy
- A01H6/46—Gramineae or Poaceae, e.g. ryegrass, rice, wheat or maize
- A01H6/4624—Hordeum vulgarus [barley]
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/102—Mutagenizing nucleic acids
- C12N15/1024—In vivo mutagenesis using high mutation rate "mutator" host strains by inserting genetic material, e.g. encoding an error prone polymerase, disrupting a gene for mismatch repair
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6858—Allele-specific amplification
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/13—Plant traits
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/156—Polymorphic or mutational markers
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- C—CHEMISTRY; METALLURGY
- C40—COMBINATORIAL TECHNOLOGY
- C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
- C40B30/00—Methods of screening libraries
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- C—CHEMISTRY; METALLURGY
- C40—COMBINATORIAL TECHNOLOGY
- C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
- C40B40/00—Libraries per se, e.g. arrays, mixtures
- C40B40/02—Libraries contained in or displayed by microorganisms, e.g. bacteria or animal cells; Libraries contained in or displayed by vectors, e.g. plasmids; Libraries containing only microorganisms or vectors
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Biochemistry (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- General Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Biomedical Technology (AREA)
- Analytical Chemistry (AREA)
- Immunology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Plant Pathology (AREA)
- Crystallography & Structural Chemistry (AREA)
- Developmental Biology & Embryology (AREA)
- Botany (AREA)
- Environmental Sciences (AREA)
- Physiology (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Cell Biology (AREA)
- General Chemical & Material Sciences (AREA)
- Medicinal Chemistry (AREA)
- Mycology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
12) INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property Organization International Bureau (43) International Publication Date 04 January 2018 (04.01.2018) W I PO I PCT IiiimmoimiolollmonoloolosolimmommovoimIE (10) International Publication Number WO 2018/001884 Al (51) International Patent Classification: C12N 15/01 (2006.01) Cl 2N 15/85 (2006.01) C12N 15/10 (2006.01) (21) International Application Number: PCT/EP2017/065516 (22) International Filing Date: 23 June 2017 (23.06.2017) (25) Filing Language: English (26) Publication Language: English (30) Priority Data: PA 2016 70485 01 July 2016 (01.07.2016) DK (71) Applicant: CARLSBERG A/S [DK/DK]; Ny Carlsberg Vej 100, 1799 Copenhagen (DK). (72) Inventors: WENDT, Toni; Sankt Nikolaj Vej 30 st. tv., 1953 Frederiksberg (DK). OLSEN, Ole; Holmblads- gade 102, 2.tv., 2300 Copenhagen S (DK). KNUDSEN, Soren; Bakkesvinget 18, 2760 Malov (DK). THOMSEN, Hanne, Cecilie; Bodil Ipsens Vej 23, 2500 Valby (DK). STRIEBECK, Alexander; Sankt Nikolaj Vej 22, 3th, 1953 Frederiksberg C (DK). SKADHAUGE, Birgitte; Linde- hojvej 31B, 3460 Birkerod (DK). RASMUSSEN, Mag- nus Wohlfahrt; Eskildsgade 66, 3.th, 1657 Copenhagen V (DK). CARCIOFI, Massimiliano; Venedigvej 7, 02 tv., 2300 Copenhagen (DK). (74) Agent: HOIBERG P/S; Adelgade 12, 1304 Copenhagen K (DK). (81) Designated States (unless otherwise indicated, for every kind of national protection available): AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. (84) Designated States (unless otherwise indicated, for every kind of regional protection available): ARIPO (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, TJ, TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG). (54) Title: METHOD TO SCREEN FOR A MUTANT WITHIN A POPULATION OF ORGANISMS BY APPLYING A POOLING AND SPLITTING APPROACH (57) : In traditional plant breeding approaches, chemical mutagen- esis may be utilized to introduce nucleotide substitutions at random in the genome of a plant, i.e. without possibilities to control the sites of nucleotide changes. Because of genome complexities, the statistical probability is ex- tremely little when it comes to fmding a predetermined nucleotide substitu- tion. The present invention, however, demonstrates how a novel, alternative use of digital polymerase chain reaction (dPCR), preferably droplet dPCR (ddPCR), is developed to exploit finding of specific nucleotide substitutions in mutated genes. The entire platform comprises a screening method with a library of mutagenized organisms, digital PCR -based systems and a set-up to propagate and analyze identified, mutated organisms. Organism library pool Fraction of organism library pool Secondary sub-pools of = organisms of library pool Sub pool total [D] i t M 1 2 / 3 I 4 Secondary sub-samples —.. from secondary sub-pools of organisms W O 20 18/00 188 4 Al Reproductio 000 0000 ( Mutant organism FIG. 1C Aliquots of secondary sub-samples gDNA analysis (Example of mutant signal in well) 9 [Continued on next page] WO 2018/001884 Al 1111111111111101110111111111111111111111011111111111110111111111111M11E110111111 Declarations under Rule 4.17: — of inventorship (Rule 4.17(iv)) Published: with international search report (Art. 21(3)) with sequence listing part of description (Rule 5.2(a))
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DKPA201670485 | 2016-07-01 | ||
| PCT/EP2017/065516 WO2018001884A1 (en) | 2016-07-01 | 2017-06-23 | Method to screen for a mutant within a population of organisms by applying a pooling and splitting approach |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| SG11201811021YA true SG11201811021YA (en) | 2019-01-30 |
Family
ID=56681913
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| SG11201811021YA SG11201811021YA (en) | 2016-07-01 | 2017-06-23 | Method to screen for a mutant within a population of organisms by applying a pooling and splitting approach |
Country Status (25)
| Country | Link |
|---|---|
| US (2) | US11884972B2 (en) |
| EP (3) | EP3693458A1 (en) |
| JP (2) | JP7208797B2 (en) |
| CN (2) | CN114959098A (en) |
| AR (1) | AR110103A1 (en) |
| AU (3) | AU2017288960B2 (en) |
| BR (1) | BR112018076429A2 (en) |
| CA (1) | CA3026016A1 (en) |
| CL (1) | CL2018003710A1 (en) |
| CO (1) | CO2019000828A2 (en) |
| CR (1) | CR20190040A (en) |
| CU (1) | CU20180158A7 (en) |
| DK (1) | DK3478832T3 (en) |
| EA (1) | EA201892809A1 (en) |
| ES (1) | ES2916221T3 (en) |
| IL (1) | IL263451B (en) |
| MA (3) | MA45531A (en) |
| MX (2) | MX2018016110A (en) |
| PE (1) | PE20190228A1 (en) |
| PL (1) | PL3478832T3 (en) |
| PT (1) | PT3478832T (en) |
| SG (1) | SG11201811021YA (en) |
| UY (1) | UY37312A (en) |
| WO (1) | WO2018001884A1 (en) |
| ZA (3) | ZA201808218B (en) |
Families Citing this family (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA3085987A1 (en) | 2017-12-28 | 2019-07-04 | Carlsberg A/S | Cereal plants with improved cell wall properties |
| CN108913698B (en) * | 2018-07-25 | 2021-10-26 | 安徽农业大学 | CAPS marker related to wheat ear germination resistance/sensibility and application thereof |
| CN110724741B (en) * | 2019-07-24 | 2020-05-19 | 艾普拜生物科技(苏州)有限公司 | Primer, probe and kit for detecting minimal residual leukemia related fusion gene |
| EP3785529A1 (en) | 2019-08-28 | 2021-03-03 | Carlsberg A/S | Barley plants with altered protein content in grains |
| EP4040946A1 (en) | 2019-10-10 | 2022-08-17 | Carlsberg A/S | Methods for preparing mutant plants |
| JP2023516327A (en) | 2020-03-02 | 2023-04-19 | カールスバーグ アグシャセルスガーブ | Barley plants with high marginal dextrinase activity |
| EP4029935A1 (en) | 2021-01-14 | 2022-07-20 | Carlsberg A/S | S. eubayanus strain and hybrids thereof |
| EP4423297A1 (en) * | 2021-10-25 | 2024-09-04 | GT Molecular, Inc. | Off-target blocking sequences to improve target discrimination by polymerase chain reaction |
| WO2023118136A1 (en) | 2021-12-22 | 2023-06-29 | BASF Agricultural Solutions Seed US LLC | Improved screening method for genome edited events |
| WO2023170272A1 (en) | 2022-03-11 | 2023-09-14 | Carlsberg A/S | Modulation of protein levels |
| WO2024105148A1 (en) | 2022-11-16 | 2024-05-23 | University Of Copenhagen | Plants with low seed glucosinolate content |
| WO2024133900A1 (en) | 2022-12-22 | 2024-06-27 | University Of Copenhagen | Improved (-)-sparteine production |
| CN117965576B (en) * | 2024-02-01 | 2024-08-27 | 北京林业大学 | King lily glycoside biosynthesis gene LlHCT1 and application thereof |
Family Cites Families (16)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3996345A (en) | 1974-08-12 | 1976-12-07 | Syva Company | Fluorescence quenching with immunological pairs in immunoassays |
| US4351760A (en) | 1979-09-07 | 1982-09-28 | Syva Company | Novel alkyl substituted fluorescent compounds and polyamino acid conjugates |
| US5188934A (en) | 1989-11-14 | 1993-02-23 | Applied Biosystems, Inc. | 4,7-dichlorofluorescein dyes as molecular probes |
| EP0942991A2 (en) * | 1996-11-26 | 1999-09-22 | Baylor College Of Medicine | Identification of inhibitors that prevent access of telomerase to chromosomal terminus |
| EP0988313A4 (en) | 1997-06-04 | 2004-11-17 | Smithkline Beecham Corp | METHODS FOR RAPID CLONING OF FULL LENGTH cDNAs |
| JP2002510506A (en) * | 1998-04-02 | 2002-04-09 | テラス ジェネティック リソーシズ，インコーポレイティド | Method for obtaining plant having genetic disorder in gene sequence |
| GB9812768D0 (en) | 1998-06-13 | 1998-08-12 | Zeneca Ltd | Methods |
| WO2003000905A2 (en) * | 2001-06-22 | 2003-01-03 | Syngenta Participations Ag | Identification and characterization of plant genes |
| GB0127564D0 (en) | 2001-11-16 | 2002-01-09 | Medical Res Council | Emulsion compositions |
| US7420105B2 (en) | 2004-03-11 | 2008-09-02 | Carlsberg A/S | Barley for production of flavor-stable beverage |
| CA3210271A1 (en) | 2008-09-23 | 2010-04-01 | Bio-Rad Laboratories, Inc. | Droplet-based assay system |
| US9005565B2 (en) | 2010-06-24 | 2015-04-14 | Hamid-Reza Jahangiri-Famenini | Method and apparatus for forming graphene |
| EP3498864B1 (en) * | 2011-03-10 | 2024-10-09 | Gen-Probe Incorporated | Methods for the selection and optimization of oligonucleotide tag sequences |
| CA2831180C (en) | 2011-04-11 | 2017-02-14 | Keith Bauer | Dna polymerases with improved activity |
| US11186843B2 (en) | 2014-02-27 | 2021-11-30 | Monsanto Technology Llc | Compositions and methods for site directed genomic modification |
| GB201411603D0 (en) * | 2014-06-30 | 2014-08-13 | Vela Operations Pte Ltd | Compositions for quantitative and/or semiquantitative mutation detection methods |
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2017
- 2017-06-23 SG SG11201811021YA patent/SG11201811021YA/en unknown
- 2017-06-23 MA MA045531A patent/MA45531A/en unknown
- 2017-06-23 EP EP20163553.9A patent/EP3693458A1/en active Pending
- 2017-06-23 AU AU2017288960A patent/AU2017288960B2/en active Active
- 2017-06-23 CA CA3026016A patent/CA3026016A1/en active Pending
- 2017-06-23 CU CU2018000158A patent/CU20180158A7/en unknown
- 2017-06-23 PE PE2018003333A patent/PE20190228A1/en unknown
- 2017-06-23 EP EP17733800.1A patent/EP3478832B1/en active Active
- 2017-06-23 EA EA201892809A patent/EA201892809A1/en unknown
- 2017-06-23 EP EP20200115.2A patent/EP3792357A1/en active Pending
- 2017-06-23 ES ES17733800T patent/ES2916221T3/en active Active
- 2017-06-23 JP JP2018569004A patent/JP7208797B2/en active Active
- 2017-06-23 WO PCT/EP2017/065516 patent/WO2018001884A1/en active Application Filing
- 2017-06-23 DK DK17733800.1T patent/DK3478832T3/en active
- 2017-06-23 PL PL17733800T patent/PL3478832T3/en unknown
- 2017-06-23 MA MA054568A patent/MA54568A/en unknown
- 2017-06-23 PT PT177338001T patent/PT3478832T/en unknown
- 2017-06-23 US US16/311,450 patent/US11884972B2/en active Active
- 2017-06-23 MA MA052594A patent/MA52594A/en unknown
- 2017-06-23 CN CN202210633145.XA patent/CN114959098A/en active Pending
- 2017-06-23 IL IL263451A patent/IL263451B/en unknown
- 2017-06-23 CR CR20190040A patent/CR20190040A/en unknown
- 2017-06-23 BR BR112018076429A patent/BR112018076429A2/en unknown
- 2017-06-23 MX MX2018016110A patent/MX2018016110A/en unknown
- 2017-06-23 CN CN201780041101.5A patent/CN109477087B/en active Active
- 2017-06-30 UY UY0001037312A patent/UY37312A/en not_active Application Discontinuation
- 2017-06-30 AR ARP170101817A patent/AR110103A1/en unknown
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2018
- 2018-12-05 ZA ZA2018/08218A patent/ZA201808218B/en unknown
- 2018-12-18 MX MX2022014965A patent/MX2022014965A/en unknown
- 2018-12-20 CL CL2018003710A patent/CL2018003710A1/en unknown
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2019
- 2019-01-29 CO CONC2019/0000828A patent/CO2019000828A2/en unknown
- 2019-11-20 AU AU2019268103A patent/AU2019268103B2/en active Active
- 2019-11-20 AU AU2019268102A patent/AU2019268102B2/en active Active
- 2019-11-25 ZA ZA2019/07775A patent/ZA201907775B/en unknown
- 2019-11-25 ZA ZA2019/07774A patent/ZA201907774B/en unknown
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2022
- 2022-05-06 JP JP2022076471A patent/JP7539428B2/en active Active
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2023
- 2023-12-12 US US18/536,784 patent/US20240182961A1/en active Pending
Also Published As
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| Publication | Publication Date | Title |
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| SG11201811021YA (en) | Method to screen for a mutant within a population of organisms by applying a pooling and splitting approach | |
| SG11201811504PA (en) | Single cell whole genome libraries and combinatorial indexing methods of making thereof | |
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| SG11201909618TA (en) | Compositions and methods for library construction and sequence analysis | |
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