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SG11201704919SA - Methods of purifying adeno-associated virus (aav) and/or recombinant adeno-associated virus (raav) - Google Patents

Methods of purifying adeno-associated virus (aav) and/or recombinant adeno-associated virus (raav)

Info

Publication number
SG11201704919SA
SG11201704919SA SG11201704919SA SG11201704919SA SG11201704919SA SG 11201704919S A SG11201704919S A SG 11201704919SA SG 11201704919S A SG11201704919S A SG 11201704919SA SG 11201704919S A SG11201704919S A SG 11201704919SA SG 11201704919S A SG11201704919S A SG 11201704919SA
Authority
SG
Singapore
Prior art keywords
associated virus
adeno
raav
aav
methods
Prior art date
Application number
SG11201704919SA
Inventor
Sandra Patricia Meriño
Haifeng Chen
Original Assignee
Alfa Wassermann Inc
Virovek Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Alfa Wassermann Inc, Virovek Inc filed Critical Alfa Wassermann Inc
Publication of SG11201704919SA publication Critical patent/SG11201704919SA/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2750/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssDNA viruses
    • C12N2750/00011—Details
    • C12N2750/14011—Parvoviridae
    • C12N2750/14111—Dependovirus, e.g. adenoassociated viruses
    • C12N2750/14151—Methods of production or purification of viral material

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Virology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Immunology (AREA)
  • Centrifugal Separators (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
SG11201704919SA 2015-01-13 2016-01-08 Methods of purifying adeno-associated virus (aav) and/or recombinant adeno-associated virus (raav) SG11201704919SA (en)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US201562102650P 2015-01-13 2015-01-13
US201562102645P 2015-01-13 2015-01-13
US201562139242P 2015-03-27 2015-03-27
PCT/US2016/012680 WO2016114992A2 (en) 2015-01-13 2016-01-08 Methods of purifying adeno-associated virus (aav) and/or recombinant adeno-associated virus (raav) and gradients and flow-through buffers therefore

Publications (1)

Publication Number Publication Date
SG11201704919SA true SG11201704919SA (en) 2017-07-28

Family

ID=56367102

Family Applications (1)

Application Number Title Priority Date Filing Date
SG11201704919SA SG11201704919SA (en) 2015-01-13 2016-01-08 Methods of purifying adeno-associated virus (aav) and/or recombinant adeno-associated virus (raav)

Country Status (6)

Country Link
US (2) US9862936B2 (en)
EP (1) EP3245218A4 (en)
CN (1) CN107428799A (en)
SG (1) SG11201704919SA (en)
TW (1) TW201630926A (en)
WO (1) WO2016114992A2 (en)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA3034527A1 (en) 2016-08-23 2018-03-01 Emmanuel John Simons Compositions and methods for treating non-age-associated hearing impairment in a human subject
IL295747A (en) 2020-02-21 2022-10-01 Akouos Inc Compositions and methods for treating non-age-associated hearing impairment in a human subject
KR20230078805A (en) 2020-11-02 2023-06-02 바이오마린 파머수티컬 인크. Enrichment process of adeno-associated virus
EP4415848A1 (en) * 2021-10-12 2024-08-21 Cytonus Therapeutics, Inc. Systems and methods for manufacturing of therapeutic cells

Family Cites Families (25)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3430849A (en) 1967-08-01 1969-03-04 Atomic Energy Commission Liquid centrifuge for large-scale virus separation
KR20050043996A (en) 1996-07-01 2005-05-11 아방티 파르마 소시에테 아노님 Method for producing recombinant adenovirus
ATE550429T1 (en) 1996-11-20 2012-04-15 Crucell Holland Bv ADENOVIRUS COMPOSITIONS AVAILABLE THROUGH AN IMPROVED PRODUCTION AND PURIFICATION PROCESS
US6989264B2 (en) 1997-09-05 2006-01-24 Targeted Genetics Corporation Methods for generating high titer helper-free preparations of released recombinant AAV vectors
US6146874A (en) 1998-05-27 2000-11-14 University Of Florida Method of preparing recombinant adeno-associated virus compositions
US20040101964A1 (en) 2000-05-10 2004-05-27 Shigemitsu Takashima Method of preparing virus vector
US6593123B1 (en) 2000-08-07 2003-07-15 Avigen, Inc. Large-scale recombinant adeno-associated virus (rAAV) production and purification
US20030114289A1 (en) 2001-11-27 2003-06-19 Merino Sandra Patricia Centrifuge with removable core for scalable centrifugation
DK1506287T3 (en) 2002-05-14 2007-08-20 Merck & Co Inc Methods for purifying adenovirus
US7419817B2 (en) 2002-05-17 2008-09-02 The United States Of America As Represented By The Secretary Department Of Health And Human Services, Nih. Scalable purification of AAV2, AAV4 or AAV5 using ion-exchange chromatography
WO2004016222A2 (en) 2002-08-16 2004-02-26 Ohio University Hepatitis c viral-like particle purification
KR20050119128A (en) 2003-03-19 2005-12-20 알파 와슬만, 인크. Separation and accumulation of subcellular components, and proteins derived therefrom
CN1842706A (en) 2003-03-19 2006-10-04 阿尔法沃塞曼公司 Separation and accumulation of subcellular components, and proteins derived therefrom
US20050189305A1 (en) 2004-02-27 2005-09-01 Kathy Forrester Processes and compositions for adenovirus purification using continuous flow centrifugation
HUE060433T2 (en) * 2004-06-01 2023-03-28 Genzyme Corp Compositions and methods to prevent aav vector aggregation
US20060287506A1 (en) * 2005-06-15 2006-12-21 Alfa Wassermann, Inc. Method for separating and concentrating biological materials using continuous-flow ultracentrifugation
WO2007127264A2 (en) 2006-04-28 2007-11-08 The Trustees Of The University Of Pennsylvania Scalable production method for aav
US10072256B2 (en) * 2006-05-22 2018-09-11 Abbott Products Gmbh Process for separating and determining the viral load in a pancreatin sample
FR2918074B1 (en) 2007-06-27 2009-10-09 Sanofi Pasteur PROCESS FOR PURIFYING A VIRAL SUSPENSION
CN101633911A (en) * 2008-07-22 2010-01-27 大连雅立峰生物制药有限公司 Purification technology applicable to mass production of human-used avian influenza vaccine
DK2529020T3 (en) 2010-01-28 2018-08-06 Childrens Hospital Philadelphia SCALABLE PREPARATION PLATF FOR CLEANING VIRAL VECTORS AND CLEANED VIRAL VECTORS FOR USE IN GENTHERAPY
CN104520421B (en) 2011-10-28 2017-05-24 北卡罗来纳-查佩尔山大学 Cell line for production of adeno-associated virus
CN103374547B (en) * 2012-07-04 2015-11-18 北京健翔和牧生物科技有限公司 A kind of preparation method of foot and mouth disease purified vaccine
CN103083654A (en) * 2012-08-27 2013-05-08 万里明 Process for preparing human diploid cell rabies vaccine through Celligen310 bioreactor
CN103721251B (en) * 2013-12-12 2016-12-07 北京科兴生物制品有限公司 The purification process of influenza virus cracking vaccine

Also Published As

Publication number Publication date
TW201630926A (en) 2016-09-01
WO2016114992A3 (en) 2016-09-22
US9862936B2 (en) 2018-01-09
WO2016114992A2 (en) 2016-07-21
US20180066237A1 (en) 2018-03-08
CN107428799A (en) 2017-12-01
US20160201086A1 (en) 2016-07-14
EP3245218A4 (en) 2018-10-03
EP3245218A2 (en) 2017-11-22

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