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SG11201603045VA - Suspension and clustering of human pluripotent stem cells for differentiation into pancreatic endocrine cells - Google Patents

Suspension and clustering of human pluripotent stem cells for differentiation into pancreatic endocrine cells

Info

Publication number
SG11201603045VA
SG11201603045VA SG11201603045VA SG11201603045VA SG11201603045VA SG 11201603045V A SG11201603045V A SG 11201603045VA SG 11201603045V A SG11201603045V A SG 11201603045VA SG 11201603045V A SG11201603045V A SG 11201603045VA SG 11201603045V A SG11201603045V A SG 11201603045VA
Authority
SG
Singapore
Prior art keywords
clustering
differentiation
suspension
pluripotent stem
cells
Prior art date
Application number
SG11201603045VA
Inventor
Benjamin Fryer
Daina Laniauskas
Marcia Blackmoore
Haiyun Wang
Kostadinka Lilova
Shelley Nelson
Elizabeth Rosocha
Original Assignee
Janssen Biotech Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US13/998,974 external-priority patent/US10377989B2/en
Application filed by Janssen Biotech Inc filed Critical Janssen Biotech Inc
Publication of SG11201603045VA publication Critical patent/SG11201603045VA/en

Links

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0603—Embryonic cells ; Embryoid bodies
    • C12N5/0606—Pluripotent embryonic cells, e.g. embryonic stem cells [ES]
    • C—CHEMISTRY; METALLURGY
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0676—Pancreatic cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P1/00—Drugs for disorders of the alimentary tract or the digestive system
    • A61P1/18—Drugs for disorders of the alimentary tract or the digestive system for pancreatic disorders, e.g. pancreatic enzymes
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P25/00—Drugs for disorders of the nervous system
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P9/00—Drugs for disorders of the cardiovascular system
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0068—General culture methods using substrates
    • C12N5/0075—General culture methods using substrates using microcarriers
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0657—Cardiomyocytes; Heart cells
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
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    • C12N2501/30—Hormones
    • C12N2501/38—Hormones with nuclear receptors
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
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    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
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    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
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  • Neurology (AREA)
  • Neurosurgery (AREA)
  • Rheumatology (AREA)
  • Heart & Thoracic Surgery (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Plural Heterocyclic Compounds (AREA)
SG11201603045VA 2013-11-01 2014-05-21 Suspension and clustering of human pluripotent stem cells for differentiation into pancreatic endocrine cells SG11201603045VA (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201361962158P 2013-11-01 2013-11-01
US13/998,974 US10377989B2 (en) 2012-12-31 2013-12-30 Methods for suspension cultures of human pluripotent stem cells
PCT/US2014/038993 WO2015065524A2 (en) 2013-11-01 2014-05-21 Suspension and clustering of human pluripotent stem cells for differentiation into pancreatic endocrine cells

Publications (1)

Publication Number Publication Date
SG11201603045VA true SG11201603045VA (en) 2016-05-30

Family

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SG11201603045VA SG11201603045VA (en) 2013-11-01 2014-05-21 Suspension and clustering of human pluripotent stem cells for differentiation into pancreatic endocrine cells
SG11201603047PA SG11201603047PA (en) 2013-11-01 2014-06-17 Suspension and clustering of human pluripotent stem cells for differentiation into pancreatic endocrine cells

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SG11201603047PA SG11201603047PA (en) 2013-11-01 2014-06-17 Suspension and clustering of human pluripotent stem cells for differentiation into pancreatic endocrine cells

Country Status (12)

Country Link
EP (2) EP3063268A4 (en)
JP (2) JP2016534731A (en)
KR (4) KR20180128529A (en)
CN (2) CN105793413A (en)
AU (4) AU2014342995C1 (en)
BR (1) BR112016009393A8 (en)
CA (2) CA2928639A1 (en)
MX (2) MX2016005657A (en)
PH (2) PH12016500782A1 (en)
RU (2) RU2689710C2 (en)
SG (2) SG11201603045VA (en)
WO (2) WO2015065524A2 (en)

Families Citing this family (20)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SG11201603045VA (en) * 2013-11-01 2016-05-30 Janssen Biotech Inc Suspension and clustering of human pluripotent stem cells for differentiation into pancreatic endocrine cells
RU2771328C2 (en) * 2016-06-23 2022-04-29 Титон Биотек, Инк. Cells expressing parathyroid hormone 1 receptor, and their use
MX2019005476A (en) 2016-11-16 2019-07-04 Cynata Therapeutics Ltd Pluripotent stem cell assay.
US10391156B2 (en) 2017-07-12 2019-08-27 Viacyte, Inc. University donor cells and related methods
WO2019018818A1 (en) * 2017-07-21 2019-01-24 Semma Therapeutics, Inc. Re-aggregation of stem cell-derived pancreatic beta cells
JP7000896B2 (en) * 2017-08-23 2022-01-19 王子ホールディングス株式会社 Cell sheet forming member, method for manufacturing cell sheet forming member, and method for manufacturing cell sheet
MX2020007071A (en) * 2018-01-05 2020-11-11 Platelet Biogenesis Inc Compositions and methods for producing megakaryocytes.
CN112041428B (en) 2018-01-18 2024-05-24 新加坡科技研究局 Method for differentiating human pluripotent stem cell lines in suspension culture
KR102115360B1 (en) * 2018-05-30 2020-05-26 주식회사 바이블리오테카 Adult stem cell culture fluid and a producing method thereof
US20200080107A1 (en) 2018-09-07 2020-03-12 Crispr Therapeutics Ag Universal donor cells
EP3975925A1 (en) 2019-05-31 2022-04-06 W.L. Gore & Associates, Inc. A biocompatible membrane composite
CA3139585C (en) 2019-05-31 2024-01-23 W. L. Gore & Associates, Inc. A biocompatible membrane composite
EP3976236A1 (en) 2019-05-31 2022-04-06 W.L. Gore & Associates Inc. A biocompatible membrane composite
CA3139292A1 (en) 2019-05-31 2020-12-03 Timothy M. BRUHN Cell encapsulation devices with controlled oxygen diffusion distances
CN114364791A (en) 2019-09-05 2022-04-15 克里斯珀医疗股份公司 Universal donor cell
CA3150235A1 (en) 2019-09-05 2021-03-11 Alireza Rezania Universal donor cells
US20220389378A1 (en) * 2019-11-22 2022-12-08 Novo Nordisk A/S Spin-aggregated neural microspheres and the application thereof
WO2022144855A1 (en) 2020-12-31 2022-07-07 Crispr Therapeutics Ag Universal donor cells
WO2022203051A1 (en) * 2021-03-25 2022-09-29 株式会社カネカ Method for producing pluripotent stem cell population
CN116218766A (en) * 2023-04-21 2023-06-06 北京意胜生物科技有限公司 Method for preparing pancreatic precursor cells, culture medium and application of pancreatic precursor cells

Family Cites Families (20)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20090115984A (en) * 2001-11-09 2009-11-10 아르테셀 사이언스, 인크. Endocrine pancreas differentiation of adipose tissue-derived stromal cells and uses thereof
EP3441459B1 (en) * 2006-08-02 2021-03-17 Technion Research & Development Foundation Limited Methods of expanding embryonic stem cells in a suspension culture
US20110151554A1 (en) * 2006-11-09 2011-06-23 Akira Yuo Method for culturing and subculturing primate embryonic stem cell, as well as method for inducing differentiation thereof
US20090053182A1 (en) * 2007-05-25 2009-02-26 Medistem Laboratories, Inc. Endometrial stem cells and methods of making and using same
ES2626656T3 (en) * 2007-07-01 2017-07-25 Lifescan, Inc. Single pluripotent stem cell culture
GB0800524D0 (en) * 2008-01-14 2008-02-20 Univ Brighton Cell culture system
US8716018B2 (en) * 2008-03-17 2014-05-06 Agency For Science, Technology And Research Microcarriers for stem cell culture
PL2310492T3 (en) * 2008-06-30 2015-12-31 Janssen Biotech Inc Differentiation of pluripotent stem cells
US8008075B2 (en) * 2008-11-04 2011-08-30 Viacyte, Inc. Stem cell aggregate suspension compositions and methods of differentiation thereof
US8895300B2 (en) * 2008-11-04 2014-11-25 Viacyte, Inc. Scalable primate pluripotent stem cell aggregate suspension culture and differentiation thereof
CN102597218B (en) * 2009-08-12 2017-10-27 国立大学法人京都大学 Method for inducing differentiation of pluripotent stem cells into neural precursor cells
JP5936134B2 (en) * 2010-06-15 2016-06-15 国立大学法人京都大学 Selection method of human induced pluripotent stem cells
WO2011160066A1 (en) * 2010-06-17 2011-12-22 Regents Of The University Of Minnesota Production of insulin producing cells
RU2627168C2 (en) * 2010-08-31 2017-08-03 Янссен Байотек, Инк. Differentiation of human embryonic stem cells
WO2012117333A1 (en) * 2011-02-28 2012-09-07 Stempeutics Research Malaysia Sdn Bhd Isolation and expansion of adult stem cells, their therapeutic composition and uses thereof
WO2013056072A1 (en) * 2011-10-13 2013-04-18 Wisconsin Alumni Research Foundation Generation of cardiomyocytes from human pluripotent stem cells
EP2794857A4 (en) * 2011-12-22 2015-07-08 Janssen Biotech Inc Differentiation of human embryonic stem cells into single hormonal insulin positive cells
US10519422B2 (en) * 2012-02-29 2019-12-31 Riken Method of producing human retinal pigment epithelial cells
EP2938722B1 (en) * 2012-12-31 2021-12-08 Janssen Biotech, Inc. Suspension and clustering of human pluripotent cells for differentiation into pancreatic endocrine cells
SG11201603045VA (en) * 2013-11-01 2016-05-30 Janssen Biotech Inc Suspension and clustering of human pluripotent stem cells for differentiation into pancreatic endocrine cells

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AU2018208717A1 (en) 2018-08-16
JP2017500013A (en) 2017-01-05
WO2015065524A3 (en) 2015-08-13
EP3063268A4 (en) 2017-11-29
KR20160079071A (en) 2016-07-05
RU2016121404A3 (en) 2018-03-29
AU2014342995C1 (en) 2018-08-09
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