SG11201403365TA - Rapid method for cloning and expression of cognate antibody variable region gene segments - Google Patents
Rapid method for cloning and expression of cognate antibody variable region gene segmentsInfo
- Publication number
- SG11201403365TA SG11201403365TA SG11201403365TA SG11201403365TA SG11201403365TA SG 11201403365T A SG11201403365T A SG 11201403365TA SG 11201403365T A SG11201403365T A SG 11201403365TA SG 11201403365T A SG11201403365T A SG 11201403365TA SG 11201403365T A SG11201403365T A SG 11201403365TA
- Authority
- SG
- Singapore
- Prior art keywords
- cloning
- expression
- variable region
- gene segments
- antibody variable
- Prior art date
Links
- 238000010367 cloning Methods 0.000 title 1
- 238000000034 method Methods 0.000 title 1
- 108090000623 proteins and genes Proteins 0.000 title 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1086—Preparation or screening of expression libraries, e.g. reporter assays
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0634—Cells from the blood or the immune system
- C12N5/0635—B lymphocytes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2317/00—Immunoglobulins specific features
- C07K2317/10—Immunoglobulins specific features characterized by their source of isolation or production
- C07K2317/14—Specific host cells or culture conditions, e.g. components, pH or temperature
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2317/00—Immunoglobulins specific features
- C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
- C07K2317/56—Immunoglobulins specific features characterized by immunoglobulin fragments variable (Fv) region, i.e. VH and/or VL
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2510/00—Genetically modified cells
- C12N2510/02—Cells for production
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Genetics & Genomics (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Biomedical Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Immunology (AREA)
- Biophysics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Physics & Mathematics (AREA)
- Plant Pathology (AREA)
- Medicinal Chemistry (AREA)
- Hematology (AREA)
- Cell Biology (AREA)
- Bioinformatics & Computational Biology (AREA)
- Crystallography & Structural Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Analytical Chemistry (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP11194861 | 2011-12-21 | ||
| PCT/EP2012/076155 WO2013092716A1 (en) | 2011-12-21 | 2012-12-19 | Rapid method for cloning and expression of cognate antibody variable region gene segments |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| SG11201403365TA true SG11201403365TA (en) | 2014-07-30 |
Family
ID=47522537
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| SG11201403365TA SG11201403365TA (en) | 2011-12-21 | 2012-12-19 | Rapid method for cloning and expression of cognate antibody variable region gene segments |
Country Status (12)
| Country | Link |
|---|---|
| US (3) | US20140302559A1 (en) |
| EP (1) | EP2794652B1 (en) |
| JP (3) | JP2015500655A (en) |
| KR (1) | KR20140104963A (en) |
| CN (2) | CN104011074A (en) |
| BR (1) | BR112014013034A2 (en) |
| CA (1) | CA2854243A1 (en) |
| HK (1) | HK1199891A1 (en) |
| MX (1) | MX354049B (en) |
| RU (1) | RU2612910C2 (en) |
| SG (1) | SG11201403365TA (en) |
| WO (1) | WO2013092716A1 (en) |
Families Citing this family (19)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| MX2011010168A (en) | 2009-04-07 | 2011-10-11 | Roche Glycart Ag | Trivalent, bispecific antibodies. |
| US9676845B2 (en) | 2009-06-16 | 2017-06-13 | Hoffmann-La Roche, Inc. | Bispecific antigen binding proteins |
| RU2015153109A (en) | 2009-09-16 | 2019-01-15 | Дженентек, Инк. | SUPERSPIRAL AND / OR BINDING PROTEIN COMPLEXES AND THEIR APPLICATIONS |
| TW201138821A (en) | 2010-03-26 | 2011-11-16 | Roche Glycart Ag | Bispecific antibodies |
| JP5758004B2 (en) | 2010-08-24 | 2015-08-05 | エフ．ホフマン−ラ ロシュ アーゲーＦ． Ｈｏｆｆｍａｎｎ−Ｌａ Ｒｏｃｈｅ Ａｋｔｉｅｎｇｅｓｅｌｌｓｃｈａｆｔ | Bispecific antibodies comprising Fv fragments stabilized by disulfides |
| SG191153A1 (en) | 2010-12-23 | 2013-07-31 | Hoffmann La Roche | Polypeptide-polynucleotide-complex and its use in targeted effector moiety delivery |
| KR101638224B1 (en) | 2011-02-28 | 2016-07-08 | 에프. 호프만-라 로슈 아게 | Antigen binding proteins |
| KR101572338B1 (en) | 2011-02-28 | 2015-11-26 | 에프. 호프만-라 로슈 아게 | Monovalent antigen binding proteins |
| JP2015500655A (en) * | 2011-12-21 | 2015-01-08 | エフ．ホフマン−ラ ロシュ アーゲーＦ． Ｈｏｆｆｍａｎｎ−Ｌａ Ｒｏｃｈｅ Ａｋｔｉｅｎｇｅｓｅｌｌｓｃｈａｆｔ | A rapid method for cloning and expressing cognate antibody variable region gene segments |
| MX2014009565A (en) | 2012-02-10 | 2014-11-10 | Genentech Inc | Single-chain antibodies and other heteromultimers. |
| MX2014014804A (en) | 2012-06-27 | 2015-02-12 | Hoffmann La Roche | Method for making antibody fc-region conjugates comprising at least one binding entity that specifically binds to a target and uses thereof. |
| JP6203838B2 (en) | 2012-06-27 | 2017-09-27 | エフ．ホフマン−ラ ロシュ アーゲーＦ． Ｈｏｆｆｍａｎｎ−Ｌａ Ｒｏｃｈｅ Ａｋｔｉｅｎｇｅｓｅｌｌｓｃｈａｆｔ | Method for selecting and creating tailor-made highly selective and multispecific targeting entities comprising at least two different binding entities and uses thereof |
| US20160075766A1 (en) * | 2013-05-21 | 2016-03-17 | Roche Diagnostics Operations, Inc. | Method for producing antibodies using ovine b-cells and uses thereof |
| CN105612182B (en) | 2013-10-11 | 2019-12-10 | 豪夫迈·罗氏有限公司 | Multispecific domain exchange consensus variable light chain antibodies |
| CN105283543A (en) * | 2014-05-19 | 2016-01-27 | 豪夫迈·罗氏有限公司 | Method for producing antibodies using ovine B-cells and uses thereof |
| PL3227332T3 (en) | 2014-12-03 | 2020-06-15 | F. Hoffmann-La Roche Ag | Multispecific antibodies |
| ES2951698T3 (en) | 2016-03-30 | 2023-10-24 | Hoffmann La Roche | B cell culture procedure |
| MX2019007356A (en) * | 2016-12-23 | 2019-09-05 | Visterra Inc | Binding polypeptides and methods of making the same. |
| JP6916884B2 (en) | 2017-01-02 | 2021-08-11 | エフ．ホフマン−ラ ロシュ アーゲーＦ． Ｈｏｆｆｍａｎｎ−Ｌａ Ｒｏｃｈｅ Ａｋｔｉｅｎｇｅｓｅｌｌｓｃｈａｆｔ | B cell culture method |
Family Cites Families (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB9815909D0 (en) | 1998-07-21 | 1998-09-16 | Btg Int Ltd | Antibody preparation |
| CN100383244C (en) * | 2003-01-07 | 2008-04-23 | 西福根有限公司 | Method for manufacturing recombinant polyclonal proteins |
| US20060051348A1 (en) | 2004-09-09 | 2006-03-09 | Jorn Gorlach | Method of producing a plurality of isolated antibodies to a plurality of cognate antigens |
| CA2622603C (en) | 2005-09-15 | 2014-06-17 | Crucell Holland B.V. | Method for preparing immunoglobulin libraries |
| CA2652392C (en) | 2006-05-19 | 2017-10-10 | Alder Biopharmaceuticals, Inc. | Culture method for obtaining a clonal population of antigen-specific b cells |
| HUE043782T2 (en) | 2007-05-21 | 2019-09-30 | Alderbio Holdings Llc | Antibodies to il-6 and use thereof |
| WO2010056898A2 (en) * | 2008-11-12 | 2010-05-20 | University Of Maryland, Baltimore | Rapid expression cloning of human monoclonal antibodies from memory b cells |
| US8609097B2 (en) * | 2009-06-10 | 2013-12-17 | Hoffmann-La Roche Inc. | Use of an anti-Tau pS422 antibody for the treatment of brain diseases |
| US20120302738A1 (en) * | 2009-11-16 | 2012-11-29 | Acyte Biotech Pty Ltd | Recombinant antibody vector |
| WO2011112566A2 (en) * | 2010-03-11 | 2011-09-15 | Abbott Laboratories | Basigin binding proteins |
| SI2400298T1 (en) | 2010-05-28 | 2013-11-29 | F. Hoffmann-La Roche Ag | Single B-cell cultivation method and specific antibody production |
| JP2015500655A (en) * | 2011-12-21 | 2015-01-08 | エフ．ホフマン−ラ ロシュ アーゲーＦ． Ｈｏｆｆｍａｎｎ−Ｌａ Ｒｏｃｈｅ Ａｋｔｉｅｎｇｅｓｅｌｌｓｃｈａｆｔ | A rapid method for cloning and expressing cognate antibody variable region gene segments |
-
2012
- 2012-12-19 JP JP2014547976A patent/JP2015500655A/en active Pending
- 2012-12-19 CN CN201280060807.3A patent/CN104011074A/en active Pending
- 2012-12-19 WO PCT/EP2012/076155 patent/WO2013092716A1/en active Application Filing
- 2012-12-19 SG SG11201403365TA patent/SG11201403365TA/en unknown
- 2012-12-19 RU RU2014128987A patent/RU2612910C2/en not_active IP Right Cessation
- 2012-12-19 EP EP12812945.9A patent/EP2794652B1/en active Active
- 2012-12-19 BR BR112014013034A patent/BR112014013034A2/en active Search and Examination
- 2012-12-19 MX MX2014007256A patent/MX354049B/en active IP Right Grant
- 2012-12-19 CN CN201910132084.7A patent/CN109810188A/en active Pending
- 2012-12-19 CA CA2854243A patent/CA2854243A1/en not_active Abandoned
- 2012-12-19 KR KR1020147016436A patent/KR20140104963A/en not_active Application Discontinuation
-
2014
- 2014-06-20 US US14/310,966 patent/US20140302559A1/en not_active Abandoned
-
2015
- 2015-01-14 HK HK15100396.3A patent/HK1199891A1/en unknown
-
2017
- 2017-04-06 US US15/480,533 patent/US20170210785A1/en not_active Abandoned
- 2017-06-14 JP JP2017116638A patent/JP6573331B2/en active Active
-
2019
- 2019-01-10 US US16/244,909 patent/US12024548B2/en active Active
- 2019-06-03 JP JP2019103602A patent/JP2019162144A/en active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| MX354049B (en) | 2018-02-09 |
| BR112014013034A2 (en) | 2017-12-19 |
| CN104011074A (en) | 2014-08-27 |
| US20170210785A1 (en) | 2017-07-27 |
| US12024548B2 (en) | 2024-07-02 |
| KR20140104963A (en) | 2014-08-29 |
| EP2794652A1 (en) | 2014-10-29 |
| WO2013092716A1 (en) | 2013-06-27 |
| JP2017221190A (en) | 2017-12-21 |
| CN109810188A (en) | 2019-05-28 |
| JP2019162144A (en) | 2019-09-26 |
| MX2014007256A (en) | 2014-08-01 |
| CA2854243A1 (en) | 2013-06-27 |
| EP2794652B1 (en) | 2017-11-15 |
| RU2014128987A (en) | 2016-02-20 |
| US20140302559A1 (en) | 2014-10-09 |
| US20190382469A1 (en) | 2019-12-19 |
| RU2612910C2 (en) | 2017-03-13 |
| JP2015500655A (en) | 2015-01-08 |
| HK1199891A1 (en) | 2015-07-24 |
| JP6573331B2 (en) | 2019-09-11 |
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