[go: up one dir, main page]
More Web Proxy on the site http://driver.im/

SE9300090D0 - AFFINITY CLEANING WITH COMPLEX BOND LIGAND - Google Patents

AFFINITY CLEANING WITH COMPLEX BOND LIGAND

Info

Publication number
SE9300090D0
SE9300090D0 SE9300090A SE9300090A SE9300090D0 SE 9300090 D0 SE9300090 D0 SE 9300090D0 SE 9300090 A SE9300090 A SE 9300090A SE 9300090 A SE9300090 A SE 9300090A SE 9300090 D0 SE9300090 D0 SE 9300090D0
Authority
SE
Sweden
Prior art keywords
target substance
ligand
matrix
affinity
binding sites
Prior art date
Application number
SE9300090A
Other languages
Swedish (sv)
Inventor
Bo Gustav Mattiasson
Igor Yurii Galaev
Original Assignee
Bo Gustav Mattiasson
Igor Yurii Galaev
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Bo Gustav Mattiasson, Igor Yurii Galaev filed Critical Bo Gustav Mattiasson
Priority to SE9300090A priority Critical patent/SE9300090D0/en
Publication of SE9300090D0 publication Critical patent/SE9300090D0/en
Priority to AU58942/94A priority patent/AU5894294A/en
Priority to PCT/SE1994/000016 priority patent/WO1994015951A1/en

Links

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Peptides Or Proteins (AREA)
  • Treatment Of Liquids With Adsorbents In General (AREA)

Abstract

Method for affinity based isolation of a first target substance, said method comprising: (i) contacting said first target substance with a matrix to which is linked a ligand having affinity for said first target substance; (ii) eluting said first target substance. The method is characterized by the ligand having been initially complexed/bound to a second target substance having weaker binding ability to the ligand than the first target substance and providing multi-point attachment to the matrix by carrying several ligand binding sites and/or other matrix binding sites on its molecule enabling adsorption of the first target substance during step (i) without the second target substance becoming eluted from the matrix. In one mode the second target substance is thermo-reactive, and steps (i) and (ii) are run at different temperatures separated by the cloud point of the second target substance.
SE9300090A 1993-01-14 1993-01-14 AFFINITY CLEANING WITH COMPLEX BOND LIGAND SE9300090D0 (en)

Priority Applications (3)

Application Number Priority Date Filing Date Title
SE9300090A SE9300090D0 (en) 1993-01-14 1993-01-14 AFFINITY CLEANING WITH COMPLEX BOND LIGAND
AU58942/94A AU5894294A (en) 1993-01-14 1994-01-13 Affinity purification by the use of a complexed ligand
PCT/SE1994/000016 WO1994015951A1 (en) 1993-01-14 1994-01-13 Affinity purification by the use of a complexed ligand

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
SE9300090A SE9300090D0 (en) 1993-01-14 1993-01-14 AFFINITY CLEANING WITH COMPLEX BOND LIGAND

Publications (1)

Publication Number Publication Date
SE9300090D0 true SE9300090D0 (en) 1993-01-14

Family

ID=20388535

Family Applications (1)

Application Number Title Priority Date Filing Date
SE9300090A SE9300090D0 (en) 1993-01-14 1993-01-14 AFFINITY CLEANING WITH COMPLEX BOND LIGAND

Country Status (3)

Country Link
AU (1) AU5894294A (en)
SE (1) SE9300090D0 (en)
WO (1) WO1994015951A1 (en)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1300679A3 (en) * 1995-06-26 2004-04-14 Perseptive Biosystems, Inc. Molecular selection and analysis
JP3737516B2 (en) 1995-06-26 2006-01-18 パーセプティブ バイオシステムズ，インコーポレーテッド Fast automated continuous flow, multidimensional molecular sorting and analysis
JP2003524680A (en) * 1999-05-11 2003-08-19 財団法人化学技術戦略推進機構 Affinity control type material using stimulus-responsive polymer and separation / purification method using the material
WO2002061406A1 (en) 2001-02-01 2002-08-08 Sigma-Aldrich Co. Improved affinity matrices with enhanced visibility for molecular pull-down and immunoprecipitation applications
FR2902799B1 (en) 2006-06-27 2012-10-26 Millipore Corp METHOD AND UNIT FOR PREPARING A SAMPLE FOR THE MICROBIOLOGICAL ANALYSIS OF A LIQUID
US8362217B2 (en) 2006-12-21 2013-01-29 Emd Millipore Corporation Purification of proteins
WO2008079302A2 (en) 2006-12-21 2008-07-03 Millipore Corporation Purification of proteins
US8569464B2 (en) 2006-12-21 2013-10-29 Emd Millipore Corporation Purification of proteins
US8999702B2 (en) 2008-06-11 2015-04-07 Emd Millipore Corporation Stirred tank bioreactor
SG171446A1 (en) 2008-12-16 2011-07-28 Millipore Corp Stirred tank reactor and method
CN107312062B (en) 2010-05-17 2021-03-16 Emd密理博公司 Stimulus responsive polymers for purification of biomolecules

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0056254A1 (en) * 1981-01-14 1982-07-21 David Eldon Wood Treatment of insoluble surfaces to inhibit nonspecific protein binding
GB8317855D0 (en) * 1983-06-30 1983-08-03 Iq Bio Ltd Biochemical detection method

Also Published As

Publication number Publication date
WO1994015951A1 (en) 1994-07-21
AU5894294A (en) 1994-08-15

Similar Documents

Publication Publication Date Title
Porath et al. Immobilized-metal affinity chromatography of serum proteins on gel-immobilized group III A metal ions
NZ507557A (en) Protein purification by ion exchange chromatography
Helting et al. Structure of tetanus toxin. II. Toxin binding to ganglioside.
FI102417B1 (en) Gold sol microparticles, methods for their preparation and use, and diagnostic kits containing them
EP0930366A3 (en) Binding domains in delta proteins
SE9300090D0 (en) AFFINITY CLEANING WITH COMPLEX BOND LIGAND
Rönnstrand et al. Purification of the receptor for platelet-derived growth factor from porcine uterus.
SE8200715L (en) PYROGEN CONTENT REDUCTION
CA2265935A1 (en) Heat shock protein complexes
CA2275489A1 (en) Oil bodies and associated proteins as affinity matrices
MX9707909A (en) Antibody purification by low-ph hydrophobic interaction chromatoggraphy.
DK0460426T3 (en) Method of Protein Purification
DK0734527T3 (en) Sandwich assay sensor assembly
CA2000545A1 (en) Process and reagent for the determination of a specifically bindable substance
FI943368A0 (en) Method for isolating lactoferrin and lactoperoxidase from milk and milk products and products obtained by the method
WO2001083515A3 (en) Simulated activity of protein a displayed by ligands attached to a cellulose bead surface
AU2002349344A1 (en) Microfluidic extraction method
WO2006016960A3 (en) Method for purifying a protein of the cystine-knot superfamily
AU5406800A (en) Method for carrying out the chromatographic fractionation of plasma or serum, preparations obtained thereby and their use
SE9802213D0 (en) A method for the removal / purification of serum albumins and means for use in the method
Zeheb et al. An analytical method for the selective retrieval of iminobiotin-derivatized plasma membrane proteins
DE3851221D1 (en) NON-DISCONNECTING SOLID PHASE ENZYME TEST.
ATE114315T1 (en) RADIOACTIVE LABELING OF PROTEINS.
EP0021050B1 (en) Process for isolating immunologically active enzyme labeled conjugates
Lannér et al. Purification of enzyme-labelled conjugate by affinity chromatography