RU2709535C1 - Method for preventing and treating parasitic diseases of farm animals and birds - Google Patents

Abstract

FIELD: veterinary medicine.

SUBSTANCE: invention refers to veterinary science, particularly to prevention and treatment of parasitic diseases of farm animals and birds. Method consists in the fact that into an animal and a bird with ecto- and/or entozoic parasite once or twice subcutaneously, or intramuscularly, or orally in a mixture with concentrated fodder or water is administered a drug containing in g per 100 ml: ivermectin – 0.4–0.6, praziquantel – 5.0–7.0, methylpyrrolidone – 24.0–26.0, benzyl alcohol – 0.5–1.5, propylene glycol – up to 100, in dose of 0.6–1.2  ml per 15–20 kg of live weight.

EFFECT: invention provides efficacy in a wide range of endo- and entozoic parasite diseases in a wide range of farm animals and birds.

1 cl, 7 tbl, 28 ex

Description

The invention relates to the field of veterinary medicine, in particular, to the prevention and treatment of parasitic diseases of farm animals and birds.

Parasitic diseases of farm animals and birds are widespread in Russia and cause great economic damage to livestock and poultry, lead to mass deaths and forced slaughter of animals and birds. So, with coccidiosis, up to 80-90% of chickens and rabbits can die; with ascariasis, weight gain in piglets is reduced by 30-50%, and egg laying of chickens - by 20-40%; with fascioliasis and babesiosis, milk yield in cows is reduced by 20-40%; when damaged by a subcutaneous gadfly, the best areas of cattle skin become unsuitable for dressing, etc. Even with weak infection, in which there are no visible signs of the disease, animals and birds lag behind in growth and development, poorly absorb food, and their productivity and performance decreases. Infection of animals with helminths most often occurs through the mouth with food or water, less often in other ways. Once in the gastrointestinal tract with water or food, helminth larvae through the blood or lymphatic system often migrate (migrate) to various organs and tissues of the animal, and the metabolic products of the parasites are often very toxic and cause poisoning of the whole organism of the animal or bird. Microbes that cause infectious diseases can penetrate through the tissue damaged by parasites. Microbes quickly develop in a weakened body of the animal, and the disease is more severe.

Particularly dangerous are parasitic diseases common to humans and animals.

Thus, the improvement of the livestock of farm animals and poultry, the search for new medicinal forms of a wide spectrum, acting both on larval stages and adult forms of parasites, is one of the most important tasks of modern veterinary medicine.

A known method for the treatment of animal helminthiases by using a medicinal product containing a compound from the group of avermectins, pyrantel or its salt, praziquantel and target additives (patent RU 2287332 C2, 11/20/2006).

Also known is a method of treating animal helminthiasis by using a medicament containing a compound from the group of avermectins, praziquantel, levamisole and targeted additives (patent RU 2367435 C1, 09/20/2009).

However, the known methods and means are not only highly effective in the treatment of helminthiasis, but also toxicity and immunosuppressive activity.

A known method of treating animal helminthiasis by using a medicinal product containing a compound from the group of avermectins, praziquantel and targeted additives (patent RU 2279874 C9, 07.20.2006).

The disadvantage of this invention is that it is a comprehensive drug for horses. This drug is made in the form of a paste and cannot be used for deworming of small and cattle.

A known method of treating parasitic diseases of waterfowl (patent RU 2412700 C1, 02/27/2011) and a method for treating parasitic diseases of ruminant animals (patent RU 2412697 C1, 02/27/2011). In known methods use a drug containing praziquantel, ivermectin and target additives. The methods can reduce the toxic effect of the drug used while maintaining its high therapeutic anthelmintic activity. However, the drug in these methods is used orally in the form of a suspension and is not suitable for use in the form of injections.

A known method for the prevention and treatment of parasitic diseases of ruminants, including intramuscular injection of a drug containing praziquantel, ivermectin and targeted additives (patent CN 101926813 A, 12.29.2010). The drug used in this method is in the form of a solution and is used for injection. The method has high efficiency.

This method is the closest to the claimed. However, the known method is used for the prevention and treatment of parasitic diseases of only ruminants. Information on the use of this tool for other animal species, as well as for farm birds, is not available. There is also no information about the possibility of using a known drug in the form of a solution for oral administration.

The objective of the invention is to provide a method for the prevention and treatment of mixed parasitic diseases caused by both endoparasites and ectoparasites in a wide range of farm animals and birds, in which it would be possible to administer the drug either by injection, or orally, individually or in a group way.

The problem is solved in that in the method for the prevention and treatment of parasitic diseases of farm animals and birds, according to the invention, animals and birds with damage to ecto and / or endoparasites are administered once or twice subcutaneously or intramuscularly, or orally in a mixture with concentrated feed or with water drug containing (in g per 100 ml): ivermectin -0.4-0.6, praziquantel-5.0-7.0, methylpyrrolidone -24.0-26.0, benzyl alcohol - 0.5-1 , 5, propylene glycol - up to 100, at a dose of 0.6-1.2 ml per 15-20 kg of live weight.

The problem is also solved by the fact that the drug is administered orally in a mixture with concentrated feed or with water to an agricultural animal and / or bird individually or in a group way.

The technical result of the claimed invention 'is the high efficiency of the claimed method in the prevention and treatment of parasitic diseases of a wide range of farm animals and birds caused by both endoparasites and ectoparasites, as well as the possibility of using the claimed drug either in the form of injections, or orally, individually or in groups way.

The invention is illustrated by the following examples, which, however, do not limit the scope of the claims of the applicant.

Example 1. Preparation of a medicinal product.

The following is loaded into a 300-liter mixer with a working mixer: praziquantel 18.0 kg, ivermectin 1.5 kg, benzyl alcohol 3.0 kg, methylpyrrolidone 75.0 kg, propylene glycol 225.0 kg.

The contents of the mixer are mixed until the components are completely dissolved and a clear solution is obtained.

The resulting solution is subjected to sterilizing filtration.

A medicine of the following composition is obtained (in g per 100 ml): praziquantel 6.0, ivermectin 0.5, methylpyrrolidone 25.0, benzyl alcohol 1.0, propylene glycol 67.5.

The finished product is poured into sterile vials and sealed.

In appearance, the drug is a clear liquid from colorless to pink. The drug is stable under the storage conditions in the manufacturer’s sealed packaging, in a place protected from direct sunlight, at a temperature of 2 ° C to 30 ° C for two years from the date of manufacture.

When conducting clinical studies of the claimed preparation, the following animal species were used: sheep, lambs, goats, calves, deer, as well as poultry - hens, geese, ducks, turkeys.

When conducting clinical trials on therapeutic efficacy, the test drug was administered subcutaneously or intramuscularly, as well as individually or in a group manner, mixed with food or with drinking water.

In choosing the doses, they were guided by the results obtained in a preclinical study of the toxicity and tolerability of the claimed drug in laboratory and target animal species, as well as data obtained in the study of the specific activity of the active substances of this drug, taking into account the development cycles of endo- and ectoparasites.

The claimed drug was used for agricultural animals once, cattle and small cattle, deer subcutaneously or intramuscularly; sheep, goats orally (orally) individually or in a group way in a mixture with concentrated feed or water; poultry - in a group way with drinking water.

Animals and birds were divided into experimental and control groups on the basis of analogues taking into account age and weight. The diagnosis of the disease was made comprehensively taking into account the epizootic situation on the farm, based on data from a clinical examination of animals and birds, laboratory tests, pathological autopsy of dead animals and birds. Before the start of the experiment, as well as after the deworming procedure, the test drug was used for diagnostic purposes coproovoscopic studies.

No anti-parasitic drugs were administered to the animals of the control groups during the experiment.

During the experiment, animals and poultry conducted daily clinical observations, taking into account the general condition, dynamics of the course of the disease, clinical condition, the presence or absence of complications, side effects, recovery time, activity, the presence or absence of appetite, food and water consumption, skin condition, coat or feather cover, conducted coproovoscopic studies. All changes were recorded in the acts and protocols of the study.

The reliability of the obtained results of the research is based on the fact that the data were obtained using the methods of collecting and processing information, as well as standard experimental methods and methods of variation statistics.

Example 2

Production experience conducted on 11 lambs. The lambs aged 4-6 months were injected with the proposed drug subcutaneously at a dose of 1 ml per 20 kg of live weight. Before the administration of the drug, feces were taken from all the lambs for coprological studies and it was found that out of 11 lambs 9 heads were invaded by moniesia (the invasion intensity - EI is 82%), the lambs gastrointestinal tracts were 82% invasive with the invasion intensity (AI) -19.5 eggs in 3 drops of suspension.

We took into account the effectiveness of the drug according to the results of coprological studies and found that the claimed drug showed 100% effectiveness against moniesia, against strong intestinal tract tracts (hereinafter referred to as the gastrointestinal tract), the efficacy (IE) was 75% with extensibility (EE) -80% .

After deworming, daily monitoring of the general physiological state of the lambs was carried out and no deviations from the norm were noted. The presence of side effects, complications and adverse reactions after the use of the claimed drug is also not detected.

Example 3

When performing experimental studies, we studied the effectiveness of the claimed drug against moniesia and strongil the gastrointestinal tract of sheep.

Ewes at the age of 3-5 years of the Romanov breed were divided into two groups according to the principle of analogues: experimental - 50 animals and control - 10 animals.

The claimed drug in the form of a solution for injection was administered to the sheep of the experimental group once subcutaneously (pre-scapular region) at a dose of 1 ml per 20 kg of animal weight. The control animals were not prescribed any drugs.

Before the experiment, feces from the ewes of the experimental and control groups were studied using coproovoscopic (according to Fulleborn) and larvoscopic (according to Berman-Orlov) methods.

The research results are presented in table 1.

As follows from the data in Table 1, moniesiosis (EI = 92%, AI = 5-9), ostertagia (EI = 100%, AI = 2-5-16), esophagostomiasis (EI = 100%, AI) are widespread in a sheep farm = 2-9-11), haberthiosis (EI = 82%, AI = 4-8-21), nematodirosis (EI = 48%, AI = 1-4) and eimeriosis (EI = 92%, AI = 4-7 -12).

In a clinical study of experimental sheep 5-8, 12, 18 and 25 days after deworming in all cases, an improvement in the general condition, an increase in appetite and motor activity of animals was established.

Clinical signs of diseases of the gastrointestinal tract in animals of the control group, namely lethargy, loss of appetite, lack of exercise, periodic diarrhea, were also detected at the aforementioned periods of research.

Laboratory studies of feces of sheep in the experimental and control groups were carried out 12 days after the application of the claimed drug.

According to the results of coproovoscopic and larvoscopic studies of animals of the experimental group, the following indicators of the extensibility of the claimed drug were established: with monieziosis EE = 96%, with strictilitis of the gastrointestinal tract EE = 90%. The eggs of Moniezia spp. Were detected only in two experimental animals, and the eggs and larvae of habertia, esophagostom, ostertagia, nematodir - in five. Two species of eimeria (Eimeria arloingi, E. ninaekohljakimovae) were found in feces samples in only 13 (EI = 26%) experimental sheep, which is apparently due to an increase in general reactivity and local immunity due to deworming.

In laboratory control animals, moniesia eggs, eggs and larvae of the gastrointestinal tract and Eimeria oocysts with the same indices of invasion intensity and intensity as at the beginning of the experiment were found in a laboratory study.

The results confirm the high anthelmintic efficacy of the claimed drug against moniesia and strongil of the gastrointestinal tract.

Side effects of the claimed drug in sheep of the experimental group has not been established.

Thus, we can conclude that the claimed drug in the form of a solution for injection containing ivermectin and praziquantel as active ingredients, as well as auxiliary components, when used once at a dose of 1 ml per 20 kg of animal weight, is subcutaneously characterized by high therapeutic efficacy with moniesiosis (EE = 96%), haberthiosis, esophagostomiasis, ostertagia and sheep nematodirosis (EE = 90%).

Example 4

Sheep spontaneously invaded by gastrointestinal strongilates were divided into 3 experimental and one control groups of 10 animals each. Sheep of the 1st, 2nd and 3rd experimental groups were injected subcutaneously with the once-declared preparation at a dose of 0.04, respectively; 0.05 and 0.06 ml / kg. Sheep in the control group did not receive the drug.

In case of moniosis, the drug was tested on 40 sheep, of which 10 animals served as a control, and the animals of 1, 2 and 3 experimental groups of 10 animals each were administered the claimed drug in a dose of 0.04, respectively; 0.05 and 0.06 ml / kg subcutaneously.

The calculation of the effectiveness of the drug was carried out in an experiment of the type "control test" by taking into account the degree of infection before and 14 days after administration of the drug based on the study of fecal samples by flotation.

Test results indicate 87.4; 94.3 and 95.2% effectiveness of the drug in a dose of 0.04, respectively; 0.05 and 0.06 ml / kg for strictilata of the digestive tract and 78.0; 87.8 and 90.9% efficacy in moniesiosis of sheep.

Thus, in this experiment, when testing the claimed drug at a dose of 0.04; 0.05 and 0.06 ml / kg, 87.4 obtained; 94.3 and 95.2% effectiveness in gastrointestinal strongilitis and 78.0; 87.8 and 90.9% efficacy in sheep moniesiosis.

The results of the study are presented in table 2.

Example 5

The test of the claimed drug in the form of a solution for injection was carried out in one of the farms of the Kursk region.

In 50 sheep, weighing from 20 to 50 kg, individual feces were taken. Samples were investigated by inspection, sequential washing, flotation according to Fulleborn and larvoscopically according to Berman-Orlov. In 23 samples of feces, eggs were found to guard the gastrointestinal tract (EI = 46%). When examining sheep for ectoparasitic diseases, sheep's bloodsuckers Melophagus ovinus (EI = 100%, AI from 3 and higher), Bovicola ovis (EI = 100%) and ixodid ticks (EI = 100%) were found in all. No eggs and segments of moniesia were found in feces.

40 sheep (experimental group) were treated with the claimed drug in a dose of 1 ml per 20 kg of mass once subcutaneously in the lower third of the inner thigh.

At the time of treatment in some animals, the perianal region is stained with dried feces. When examining the freshly excreted feces of all animals before deworming, segments of moniesia were not found. 10 sheep of the control group were not subjected to processing.

After deworming, animals were released for grazing. 6 hours after deworming, the discharge of moniesia began up to 80 cm long. The departed helminths were of the premaginal stage of development: with heads, milky-white in color, alive (when captured with tweezers, the strobe was reduced). The passage of parasites was observed for another 3 hours, after which the observation of the animals was stopped. The departed moniesius

collected, washed in water and fixed with 70 ° alcohol. They were later identified as Moniezia expansa. Thus, the diagnosis of moniesiosis in this farm was established in the premaginal period of the development of moniesia by deworming in all animals taken in the experiment (EI = 100%).

Within 9 hours after deworming, during the period of discharge of moniesia, as well as in the subsequent period, negative changes in the condition and behavior of sheep did not occur. During the observation, it was found that the appetite and general condition of the animals improved.

Three weeks after the administration of the claimed medicinal product, all the sheep of the experimental group were thoroughly examined for the presence of bloodsuckers, water-eaters, and ixodid ticks. In the same period, all the sheep of the control group were carefully examined. All animals of the experimental group of bloodsuckers, water-eaters, ixodid ticks and other ectoparasites were not found - extensibility and intensification in melophagosis, bovicolezis and ixodidosis was 100%. In all 10 sheep of the control group, bloodsuckers and lice eaters were found on the body; in 4 - living sucking ixodid ticks. At the same time, individual samples of feces were taken from all the sheep of the experimental and control groups. During their study, eggs and larvae of strong gastrointestinal tract, eggs and segments of moniesia in samples of sheep from the experimental group were not found. Thus, the extensibility and intensification in strictilosis of the gastrointestinal tract and moniesiosis amounted to 100%. Strong sheep type eggs (EI = 100%) were found in all the sheep of the control group in feces, and moniesia segments (EI = 80%) were found in 8 samples.

On the same day, in order to control the extensibility and intensity of the claimed preparation according to moniesiosis, all the sheep taken in the experiment were treated with this preparation in the form of a solution in a dose of 1 ml per 10 kg of weight once individually on an empty stomach and watched for 10 hours . In the indicated period, as well as in the next two days, no discharge of moniesia from the intestines of animals of the experimental group was observed, which confirms the 100% extensibility and intensity of the claimed drug for moniesiosis. In all the sheep of the control group, after 5-6 hours, the discharge of moniesia was observed.

The test results of the claimed drug in a dose of 1 ml per 20 kg of sheep weight once subcutaneously indicate:

-100% extensibility and intensification of the drug at the indicated dose in case of sheep melophagosis and bovicolezis;

-acaricidal and protective actions of the claimed drug on ixodid ticks;

-100% extensibility and intensification in cases of moniesiosis and strictilatoses of the gastrointestinal tract of sheep;

- the absence of this drug in a specified dose of side effects on sheep.

Example 6

The claimed medicinal product was tested in the form of an injection for 28 Texel sheep and mixed breeds with it from 4 months to 6 years old weighing from 15 to 80 kg. Individual sheep feces were taken from all sheep. Samples were investigated by inspection, sequential washing, flotation according to Fulleborn and larvoscopically according to Berman-Orlov. In all 28 samples of feces, eggs were found to guard the gastrointestinal tract (EI = 100%). When examining sheep for ectoparasitic diseases, sheep’s blood suckers Melophagus ovinus (EI = 100%, AI from 6 and above), Bovicola ovis (EI = 100%), and ixodid ticks (EI = 100%) were found. Eggs and segments of moniesia were not found in feces, but, according to the owner of the sheep, in past years, when killed in the intestines of sheep, moniesia was found, and in feces - segments of moniesia. All livestock was treated with the claimed drug in a dose of 1 ml per 20 kg of mass once subcutaneously in the lower third of the inner surface of the thigh. At the time of treatment in some animals, the perianal region is stained with dried feces. One lamb weighing 19 kg showed lethargy, diarrhea, and the thighs were stained with liquid feces. When examining freshly excreted feces from all animals to deworming, segments of moniesia were not found.

After deworming, the animals were grazed for 5 hours, and then returned to the pen, where they were given grain fodder and water. An hour after that, i.e. 6 hours after deworming, the discharge of moniesia began up to 80 cm long. The departed helminths were of premaginal stages of development: with heads, dense, light yellow in color, alive (when capturing with tweezers, the strobe was reduced), i.e. looked like M.benedeni. The passage of parasites was observed for another 2 hours, after which the observation of the animals was stopped. The retracted moniesia was collected, washed in water and fixed with 70 ° alcohol. Thus, the diagnosis of moniesiosis in this farm was established in the premaginal period of the development of moniesia by deworming in all animals taken in the experiment (EI = 100%).

During the first 8 hours after deworming, during the period of departure of moniesia, and in the subsequent period - within a month - according to our observations and the words of the owner, there were no negative changes in the condition and behavior of the sheep. The appetite and general condition of the animals improved.

In addition, all 28 sheep treated with the claimed preparation were thoroughly examined for the presence of bloodsuckers, water-beetles, and ixodid ticks. All animals of these and other ectoparasites did not have -extensibility and intensification by melofagosis, bovicolezis and ixodidosis was 100%. At the same time, individual samples of feces were taken from all sheep. In their study of eggs and larvae, strong gastrointestinal tract, eggs and segments of moniezia were found. Thus, the extensivity and intensivity of strictility of the gastrointestinal tract and moniesiosis amounted to 100%.

The results of a test of the claimed drug in sheep at a dose of 1 ml per 20 kg of animal weight conducted on sheep once subcutaneously testify to the 100% extensibility and intensity of this drug in case of moniesiosis, gastrointestinal strictility, melophagosis, bovicolese and ixodidosis, as well as the absence of this drug in the specified dose of side effects on the sheep.

Example 7

For trial processing, 2 sheep of the "local" breed, each weighing 40 kg, were selected. Sheep are clinically healthy, sheared, have not undergone antiparasitic treatments this year, have been grazing since the end of April. Individual feces were collected. In a subsequent study of feces by sequential washing, flotation in a saturated sodium chloride solution and the simplified Berman method according to Shilnikov, both guild eggs and larvae of the gastrointestinal tract were found in both samples. Each sheep was injected with the claimed drug at a dose of 1 ml per 20 kg of mass, i.e. 2 ml per head, subcutaneously in the lower third of the inner thigh. During the first eight hours of direct observation of the animals and during the subsequent time, no changes in the general condition of the animals were noted.

6 hours after the administration of the drug with unformed sheep feces, the premaginal forms of moniesia with a length of 30-50 cm in the amount of 3 specimens departed.

Additionally, 28 heads of “local” breed sheep weighing from 10 to 40 kg were dewormed. The sheep is in satisfactory condition; in some individuals, the perianal region is stained with feces. The drug was administered at a dose of 1 ml per 20 kg of mass once subcutaneously in the lower third of the inner surface of the thigh. Immediately after deworming, the sheep were released into the territory adjacent to the clinic for grazing.

6 hours after the administration of the drug, the discharge of moniesia from 10 to 60 cm long began. Part of the departed moniesia was collected with tweezers, washed with water and placed in a jar with 70 ° alcohol. The species affiliation of moniesia was not determined. The departure of moniesia was observed for another two hours of observation, after which the sheep were driven into the room. In the morning, fragments of strobes moniesia were also found on the floor of sheep-keeping machines in their feces. The species affiliation of moniesia was also not determined.

During the first eight hours of direct observation and over the next seven days, there were no negative changes in the general condition of the animals.

20 days after the treatment, eggs, segments and helminth larvae in the samples of feces of sheep did not reveal methods of inspection, sequential washing, flotation in a solution of sodium chloride and Shilnikov's method.

Example 8

When examining 9 samples of feces of young sheep belonging to a private individual, strong larvae were found in 6 samples of larvae. In the study of 20 samples (second batch) of feces of sheep of different ages from the same farm, Strongylide type eggs were found in 17 samples, in 2 samples samples - Dictyocaulus filarial larvae. According to the owner, in previous years, segments of moniesia were found in the feces of sheep. The farm contains about three hundred heads of Romanovskaya, fat tail and crossbred sheep aged from 2 weeks to 5 years old, weighing from 5 to 60 kg. Sheep graze on dry and moist natural pastures.

We performed deworming with the claimed medicinal product of 120 young animals weighing from 15 to 30 kg in a dose of 1 ml per 20 kg of mass once subcutaneously in the lower third of the inner thigh. At the time of treatment, the majority of the animals were clinically healthy. In single sheep, coughing was observed; in some, the perianal region and the inner thighs were stained with feces.

6 hours after deworming, the mass exodus of premaginal forms of moniesia up to 80 cm long began. No anxiety or deterioration of the general condition of the animals was observed. According to the owner, the departure of moniesia was observed until the evening. 3-4 days after deworming, coughing stopped, sheep became more alert, appetite improved.

17 days after deworming, individual feces were collected from 20 treated animals. When testing samples by flotation in a saturated solution of sodium chloride and the simplified Berman method according to Shilnikov, eggs and larvae of strongilates were not detected.

Example 9

In the study of 11 samples of feces of sheep aged 4 months to 1.5 years by sequential washing, flotation in a saturated solution of sodium chloride and the simplified Berman method in the Shilnikov modification in 2 samples, Dicrocoelium lanceatum eggs were found, in 5 egg samples Strongylid type and larvae of strong gastrointestinal tract. The farm has about 400 sheep of different ages of Romanov and crossbreeds. At the time of taking the feces, the animals looked clinically healthy, no discharge of moniesia segments was observed, antiparasitic treatments were not carried out this year.

30 heads of young animals weighing 20-25 kg and 10 heads of older sheep weighing 50 kg were treated with the claimed preparation at a dose of 1 ml per 20 kg of mass once subcutaneously in the lower third of the inner thigh.

The discharge of moniesia began after 5.5 hours and lasted for 2-3 hours. 10 days after deworming in 10 samples of feces taken from treated sheep, eggs and helminth larvae were not found.

Example 10

In the study of 10 samples of feces of sheep belonging to a private person by sequential washing, flotation in a saturated solution of sodium chloride and the simplified Berman method in the Shilnikov modification in 6 samples, Strongilide type eggs and larvae of the gastrointestinal tract were detected

The farm has 19 outbred sheep (5 sheep, 1 sheep, 13 young animals weighing 10 to 80 kg). Antiparasitic treatments were not carried out this year.

All sheep (19 goals) were treated with the claimed preparation in a dose of 1 ml per 20 kg of weight once subcutaneously in the lower third of the inner thigh.

After 5.5 hours, the premaginal forms of M. expansa moniesia began (strobile gentle narrow white with a bluish tint) stroking up to 60 cm long. The departed moniesia were collected and fixed in 70 ° alcohol. Departure continued for 2 hours of direct observation and, according to the owner, later.

A day after deworming, the sheep became more active, appetite improved.

10 days after deworming in the study of 10 samples of feces, helminth infections were not detected.

Based on the results of the studies presented above, it can be concluded that the claimed preparation at a dose of 1 ml per 20 kg of mass with a single subcutaneous injection into the hairless place of the lower third of the inner thigh of Texel sheep, Romanovskaya breed, fat tail and crossbred sheep ( outbred) weighing from 10 to 80 kg, does not have a negative effect on the general condition of animals, has a pronounced effect on the gastrointestinal tract strictile, dictyocaulus and premaginal forms of moniesia, while side effects are complicated No adverse reactions after administration of the drug have been identified.

Example 11

30 samples of feces of ewes of different age groups were taken. Samples of feces were examined on the day of collection. Samples were examined by the inspection method to detect moniesia segments, the standardized flotation method in a saturated sodium chloride solution (according to Füllenborn) to detect eggs of cestodes and nematodes, and the Berman-Orlov method to detect dictiocaulus larvae. Dictyocaulus larvae were verified by staining with methylene blue, as well as by the presence of a button-like thickening at the front end. In sheep of the experimental group (20 goals), moniesia eggs were found in 5 samples (EI - 25%), eggs strongly gastrointestinal tract in 19 samples (EI - 95%), dicticulus larvae in 7 samples (EI - 35%). In sheep of the control group (10 animals), moniesia eggs were detected in 6 samples (EI - 60%), eggs strongly gastrointestinal tract in 7 samples (EI - 70%).

An experimental group of sheep in the amount of 20 animals was treated with the claimed preparation in a dose of 1 ml per 20 kg of animal weight once orally from a dosing syringe individually in the morning on an empty stomach. Sheep in the control group (10 animals) was not treated with anything. At the time of treatment, the majority of the animals were clinically healthy, but several animals had a cough and signs of diarrhea.

After treatment with the claimed preparation, the animals were left for 24 hours in the pricked tyrl to monitor their condition and cestode discharge time. Feeding and drinking were not limited. Fecal cestodes started 6 hours later and continued for the next 2 hours, after which the monitoring of the animals was stopped. Along with fragments of strobiles, parasite heads departed. Part of the isolated cestodes was collected, washed in water and fixed in Barbagallo liquid. They have been identified as Moniezia benedeni. Within 8 hours after treatment and over the next two weeks, according to our observations and the observation of the owner of the sheep, no negative changes in the condition and behavior of the treated animals were noted. According to the owner, the appetite and general condition of the animals treated with the claimed preparation improved, coughing and diarrhea stopped.

10 days after processing the sheep, 20 samples of feces of the ewes of the experimental group and 10 samples of feces of the ewes of the control group were examined. In the study of feces by inspection methods, flotation according to Fullenborn and Berman-Orlov in the samples of the experimental group, segments, eggs and larvae of helminths were not detected. In 6 samples of sheep of the control group, moniesia eggs (EI - 60%) were found, in 7 samples - eggs were strong gastrointestinal tract (EI-70%).

Thus, the effectiveness of the claimed drug in a dose of 1 ml per 20 kg of mass when administered once orally individually from a dosing syringe in the morning on an empty stomach with moniesiosis and strong gastrointestinal tract was 100%.

Based on the results of studies of the claimed medicinal product carried out on sheep, when used in a dose of 1 ml per 20 kg of animal weight once orally individually from a dosing syringe in the morning on an empty stomach, we can conclude that:

- the claimed drug has 100% effectiveness in the specified dose for moniesiosis and strong gastrointestinal tract;

- after the administration of this drug in the specified dose in animals, there are no side effects, complications and undesirable reactions.

Example 12

Adult sheep of 2-5 years of age of Romanov breed were divided into two experimental groups of 36 animals each (72 in total) and one control group - 10 animals. When conducting studies prior to the experiments, the general condition of the animals, body temperature (selectively), appetite, physical activity, and symptoms of the primary disease were evaluated.

Clinical examination of sheep from the experimental and control groups showed symptoms of melophagosis, namely, hair loss, focal dermatitis, pruritus, forced body position, Melophagus ovinus imago and their pupae attached to the hair, as well as a significant decrease in body weight. Diarrhea, tenesmus, and other signs of impaired gastrointestinal function have not been established.

As a result of coproovoscopic and larvoscopic studies of adult sheep of the experimental and control groups, eggs and larval stages of nematodes from the families Strongylidae (Chabertia ovina), Trichostrongylidae (Ostertagia spp., Nematodirus spathiger), Trichonematidae (Oesophagostomum bumphloemomphunomleben) were found. (Strongyloides papillosus).

The claimed preparation in the form of a solution for injection was administered once subcutaneously at a dose of 1 ml per 20 kg of body weight.

For animals of the control group, the claimed drug in the above dose was used after the completion of the experiment.

When examining animals after using the claimed drug, the timing of clinical recovery and the duration of the rehabilitation period were clarified.

In the first experimental group of sheep, 4-6 days after the use of the drug, an improvement in general condition, an increase in appetite was noted, and with an individual study of the coat - the absence of bloodsucker flies, small alopecia in the back and on the sides. Moreover, in animals of the first experimental group, symptoms of melophagosis were less pronounced in comparison with the second before the start of the experiment.

In the second experimental group of sheep for 7-12 days, despite their satisfactory condition, good appetite and increased motor activity, hair loss was still observed, and extensive alopecia was detected in the neck, back and limbs. An individual examination of sheep imago flies did not reveal Melophagus ovinus flies, but revealed a large number of non-viable pupae. It should be borne in mind that in this group, before the use of an antiparasitic drug, similar clinical signs of melophagosis were found in most animals, but in a manifest form.

In laboratory coproovoscopic and larvoscopic studies of adult sheep of the experimental and control groups, the following results were established 12-14 days after the use of the claimed preparation: in two experimental groups, five sheep (two of the first and three of the second) determined the larval stages of Bunostomum phlebotomum (1- 2 specimens), Oesophagostomum spp. (1-3 specimens each), as well as larvae of the 1st stage Strongyloides papillosus. In 10 sheep of the control group, eggs and larvae of strong gastrointestinal tract (Chabertia ovina, Ostertagia spp., Nematodirus spathiger, Oesophagostomum spp., Bunostomum phlebotomum) and rhabdiazide (Strongyloides papillosus) in large numbers (from 27 to 340 to 340) were detected ml of feces suspension). The data obtained are presented in table 3.

Note: in an entomological parasitological study of sheep from two experimental groups for melophagosis, 8-12 days after the use of the claimed preparation, no mature blood-sucking flies were found, and the pathology characteristic of the disease was recorded. In 5 out of 10 sheep of the control group, adults of Melophagus ovinus from 5 to 20 ind. per animal.

Based on the results of regular studies, it can be concluded that the clinical recovery time for the sheep of the experimental groups after application of the claimed drug is 12-15 days, and the full rehabilitation period is from 20 to 30 days.

According to the results of epizootological monitoring, the optimal terms of treatment and preventive measures for mixed forms of intestinal nematodoses and entomoses in adult sheep have been found.

Thus, the claimed drug at a dose of 1 ml per 20 kg of animal weight when administered subcutaneously has a pronounced nematodicidal effect, is effective for larval and imaginal forms of strictilatosis of the gastrointestinal tract and strongyloidosis of adult sheep, as well as for mature flies - the blood sucker Melophagus ovinus and their pupae . In the specific treatment of enterocolitis of nematode etiology, the period of clinical recovery of adult sheep is 12-15 days, and the rehabilitation period is 20-30 days.

Example 13

In a dysfunctional helminthiasis of ruminant animals, 1 experimental group of sheep was formed - 30 goals and 1 control group of sheep

- 30 goals. A coprological examination of the feces of sheep in the experimental and control groups revealed a 75% damage to the sheep with helminths (EI

- 75%). The diet for animals of all groups was the same. The mass of sheep during the experiment ranged from 20 to 50 kg.

The experimental group of sheep introduced the claimed drug once intramuscularly at a dose of 1 ml per 15 kg of animal weight. During intramuscular administration of the claimed preparation to experimental animals, no inflammatory processes were observed at the injection site of the preparation. Control animals of the claimed drug and other antiparasitic drugs were not received.

During the experiment, the sheep of the experimental and control groups grazed on pastures. Within 3 days after administration of the drug, the state of health of the animals of the experimental group was monitored

The effectiveness of the claimed drug was taken into account according to the results of a study of samples of sheep feces before and 18 days after administration of the drug. The calculation of the effectiveness of the drug was carried out in an experiment of the type “control test” according to the Guidelines approved by the World Association for the Progress of Veterinary Parasitology (1995). From the experimental and control groups of sheep 18 days after the administration of the claimed drug, 3 heads were killed for the purpose of an autopsy.

18 days after the administration of the claimed preparation in a dose of 1 ml per 15 kg of the animal’s weight, the sheep of the experimental group of eggs of fasciol and dicrocelium in 1 g of feces found 4.6 ± 1.8 specimens. In the feces of sheep of the control group, the number of fasciolus eggs and dicrocelia averaged 257.8 ± 2.4 specimens. The effectiveness of the claimed drug in sheep against trematodes was 98%. At the dissection of the intestines of 3 sheep of the control group, 19 fasciol and 24 dicrocelia were found, on average 15 specimens per animal. Two sheep of the experimental group were 100% free from trematodes; 2 fasciols were found in the liver of one sheep in the liver. After the introduction of the claimed drug in a dose of 1 ml per 15 kg of weight, the sheep of the experimental group of eggs are strongly digestive, eggs nematodir and eggs trichocephalus in 1 g of feces were not detected. The intensity of the claimed preparation against sheep nematodes was 100%, while in the feces of the sheep of the control group the eggs of the strong intestinal tract were counted 218.5 ± 7.8; nematodir eggs 165.4 ± 11.2; eggs trichocephalus 104.3 ± 14.5.

When deworming animals of the experimental group of the claimed drug, adverse reactions at the injection site were not noted.

Fragments were strobed and individual segments of cestodes of the genus Moniezia were absent in the feces of animals from the experimental groups.

The test results of the claimed drug indicate its high efficiency.

The dose of the claimed drug is 1 ml per 15 kg of animal weight, at which an intensity efficiency of 100% for moniesiosis and nematodoses and 98% for sheep trematodoses was obtained, is recommended as therapeutic.

The results of studies of the anthelmintic efficacy of the claimed drug give reason to recommend this drug in veterinary practice for the treatment and prevention of moniesiosis and trematode doses of small cattle in order to prevent significant economic damage.

There was no negative effect of the claimed preparation on the animal organism, no side effects, complications and adverse reactions to the administration of the preparation were revealed.

Example 14

According to the principle of analogues, 2 groups of sheep were formed - experimental (22 heads) and control (10 goals).

Feces were taken from the experimental and control sheep for coproscopic examination for helminthiases.

In the study of fecal samples, spontaneous infection of sheep with strong gastrointestinal tract gastrointestinal tract, dictiocauliasis, dicroceliosis, trichocephaliasis, moniesiosis was established. All sheep underwent a clinical examination. The general condition of the main livestock of sheep is satisfactory, the fatness is average. In 14 young animals, a cough was noted that intensified during the run, mucous discharge from the nasal openings, lethargy; 7 goals showed moderate diarrhea, excretion of moniesia segments with liquid feces. When examined for the presence of ectoparasites on the body, all 32 animals found a bloodsucker Melophagus ovinus (AI from 10 to 40) and Bovicola bovis lice eaters (average AI - 1-3 per 10 cm ² ).

Prepared feed mixture based on the claimed drug for feeding to sheep in a group way.

The method of preparation of the feed mixture:

a) determine the total mass of the group of sheep;

b) determine the amount of the claimed drug per group, based on the dose of 1 ml per 20 kg of live weight, and increase it by 20-25%;

c) determine a single amount of grain per group, based on the norm of 200-300 g per head and the amount of water - 1/2 of the mass of grain;

g) mix a measured amount of the claimed drug with water to obtain a working solution;

d) mix the working solution of the drug with grain in a ratio of 1: 2 and leave it for 8-12 hours to soak the grain and dry.

After the solution was completely absorbed by the grain and dried up (in this farm the sheep are reluctant to eat wet grain), the resulting feed-drug mixture was poured into the trough-feeder and the experimental group of sheep were fed from the evening. Sheep willingly ate the whole feed-medicinal mixture for 20 minutes, after which they went to the meadow. Then barley without preparation was poured into the feeder and the sheep of the control group were allowed to go. After 5 hours, all the sheep returned to the spout at the watering hole, where they were closed. After 6 hours from the moment of eating the feed mixture, with the feces of the sheep of the experimental group, the discharge of moniesia began (mainly in the form of fragments of strobilus). Departure of moniesia occurred within 3 hours of observation, after which the observation of animals was stopped. According to the owner of the sheep, the departure of moniesia was observed until the evening. The retreating moniesia was collected, washed in water and fixed in 70 ° alcohol. Parasites have been identified as Moniezia benedeni, the causative agents of the so-called “autumn moniesiosis”.

During the first 9 hours after deworming and in the subsequent period - for three weeks - there were no negative changes in the condition and behavior of the sheep of the experimental group. According to the observations of the owner, the condition of the sheep of the experimental group improved significantly 2-3 days after deworming - the sheep became more active, appetite improved, diarrhea and cough stopped. The condition of the sheep in the control group has not changed. When examining the sheep also noted a significant improvement in the condition of the animals of the experimental group. The number of bloodsuckers and water-eaters on them has significantly decreased. The condition of the sheep in the control group did not change for the better.

2 weeks after treatment with the claimed preparation, 10 samples of feces were taken from the experimental and control groups of sheep. When they were studied in the laboratory by the methods of successive washing, flotation according to Fullenborn, Shilnikov, eggs of dicrocelium were found in 2 samples of the experimental group of sheep. The eggs are strong gastrointestinal tract, trichocephalus, moniesia, larvae of dictiocaulus are not found. In the control group, eggs of dicroceliums were detected in 5 samples, gastrointestinal tracts in 10 samples, trichocephalus in 6 samples, eggs and (or) segments of moniesia in 8 samples, larvae of dictiocaulus in 4 samples. The clinical condition of all the sheep of the experimental group corresponded to the norm. Sheep in the control group still showed lethargy, coughing, diarrhea, allocation of moniesia segments, poor appetite. When examining the sheep for the presence of ectoparasites, the sheep of the experimental group of mobile forms of bloodsuckers and water-eaters were not found, however, up to 20-30 pups of bloodsuckers and empty puparia were found on the rune. On the sheep of the control group, as before, motile bloodsuckers, water-eaters, and also bloodsucker pupae were found. At the request of the owner of the sheep, and also taking into account the detection of the experimental group of bloodsucker pupae on the sheep (apparently postponed until processing), a feed-and-drug mixture was prepared with the claimed preparation according to the method described above for all flocks of sheep.

All sheep from the experimental (repeated) and control (primary) groups were treated with a feed-drug mixture. There was no negative effect of the drug on animals; no side effects, complications, and adverse reactions to drug administration were established. 6 hours after treatment in animals of the control group, discharge with feces of moniesia fragments was observed. In the experimental group this was not observed. 30 days after the first treatment and 15 days after the second treatment, the sheep of the experimental group were examined for the presence of ectoparasites - no bloodsuckers and lice-eaters were found (on some sheep there were single empty pupars). In the fleece of the sheep of the control group, treated primarily, single mobile bloodsuckers (apparently only emerging from pupae), pupae and empty puparia were found; there were no whipworms. Upon examination, the general condition of the sheep of the experimental group is good, there are no clinical signs of disease. The condition of the sheep in the control group also improved significantly. 10 samples of feces were taken from the sheep of both groups. In a laboratory study in 2 samples from the experimental group and in 3 from the control, eggs of dicrocelium were found. No eggs, segments and larvae of other helminths were found.

Based on the results of experiments on 32 sheep, spontaneously invaded by moniesiosis, strictilatoses of the gastrointestinal tract, trichocephaliasis, dictiocauliasis, melophagosis, bovicolezis, the following conclusions are made:

- the claimed preparation in a dose of 1 ml per 20 kg of live weight of sheep once in a group manner in the form of a feed preparation with grain (barley) on an empty stomach has high efficiency (EE100%) for cestodoses (moniesiosis), nematodoses of the gastrointestinal tract and lungs (strongilitis, trichocephaliasis , dictiocauliasis), as well as entomoses (melophagosis, bovicolese);

- taking into account the development cycle of sheep’s bloodsucker Melophagus ovinus and other ectoparasites, for ectoparasitic diseases of sheep, the claimed preparation is advisable to be used twice with an interval of 14 days;

- negative side effects of the drug are not observed;

- the claimed preparation in the above dose in the form of a feed preparation with grain is well eaten by sheep and does not cause negative reactions.

Example 15

According to the principle of analogues, 2 groups of sheep were formed - an experimental of 20 goals and a control of 10 goals. The condition of the sheep is satisfactory; in some, the perianal region is stained with feces. Individual animals collected feces from all animals. Samples were studied by sequential washing, flotation in a saturated solution of sodium chloride and the simplified Berman-Orlov method modified by Shilnikov. Strongylide type eggs and larvae of the gastrointestinal tract were detected in all samples. When examined in 12 sheep of the experimental group (EI = 60%) and 5 sheep of the control (EI = 50%), mature sheep bloodsuckers Melophagus ovinus were found in an amount of 2 to 8 individuals (a month before the study, the sheep were sheared, and the bulk of the parasites were removed with hair). All the sheep of the experimental group were injected with the claimed preparation at a dose of 1 ml per 20 kg of mass subcutaneously in the lower third of the inner thigh. After the introduction of the drug, the sheep were released into the territory adjacent to the clinic for grazing. During the first eight hours of direct observation of the animals, as well as over the next seven days, no negative changes in the general condition of the animals were noted.

6 hours after the administration of the drug, the discharge through the anus of moniesia began from 10 to 60 cm long. The departed helminths were with heads and, judging by the size, were premaginal, i.e. immature forms. The discharge of moniesia from the intestines of the sheep of the experimental group was observed for another two hours of observation, after which the sheep were driven into the premises and the observation was stopped. Part of the departed moniesia was collected with tweezers, washed with water and placed in a jar with 70 ° alcohol. In the morning, fragments of strobes moniesia were also found on the floor of the machine for keeping sheep in feces. Thus, moniesiosis was diagnosed at the premaginal stage by deworming, which in this case was both therapeutic and diagnostic.

20 days after the treatment, individual feces samples were taken from all the sheep from the experimental and control groups. When they were studied by sequential washing, flotation in a saturated sodium chloride solution and the simplified Berman-Orlov method as modified by Shilnikov, no experimental eggs, segments and larvae of helminths were found in the feces of sheep - the deworming efficiency for gastrointestinal strictiloses was 100%.

In all samples of sheep of the control group, as before, eggs and larvae were found to guard the gastrointestinal tract, and in six samples, eggs and segments of moniesia were also found. When examined for ectoparasites, sheep’s bloodsuckers were found in all sheep of the control group: in the experimental group, single bloodsuckers were found in 4 animals — the treatment efficiency for melophagosis was 80%. Since the sheep of the experimental and control groups were kept together, the bloodsuckers found on the experimental animals could crawl from the control shortly before the examination. Since the introduction of the claimed drug signs of negative side effects of it on the sheep of the experimental group was not observed. On the contrary, the sheep became more mobile, the appetite improved.

In accordance with the foregoing, the results of a test of the drug of the claimed drug in sheep at a dose of 1 ml per 20 kg of animal weight once percutaneously indicate:

- 100% extensibility and intensification of the drug with strong gastrointestinal tract gastrointestinal tract and sheep moniesiosis;

- high (not less than 80%) drug extensivity in case of sheep melophagosis;

- the absence of this drug in a specified dose of side effects on sheep.

Example 16

The experimental and control groups selected 30 lambs of the North Caucasian breed with a live weight of 25-30 kg and with high estrosis infection. The diagnosis was made according to the clinical signs and the autopsy results of two dead animals - the detection of larvae of the nasopharyngeal gadfly Oestrus ovis in the nasal passages.

Animals were divided into 2 experimental groups (No. 1 and No. 2), 10 sheep in each and a control group (also 10 animals). Before administration of the drug and 14 days after 5 animals of the first experimental group took blood for hematological and biochemical studies.

The animals of the experimental group No. 1, the claimed drug was administered intramuscularly at a dose of 1 ml per 20 kg of live weight once.

The animals of the experimental group No. 2, the claimed preparation was set in a group way in a mixture with half a single rate of concentrated feed, which was laid out in the feeders, at a dose of 1 ml per 20 kg of animal weight, provided the animals with free access to them.

The physiological state of the animals was recorded before drug administration and 14 days after drug administration. The introduction of the claimed drug once intramuscularly and in a group way all the sheep of the experimental groups were well tolerated, side effects, complications, adverse reactions after administration of the drug were not detected.

14 days after the administration of the drug at a dose of 1 ml per 20 kg of body weight of the animal, a clinical examination of the sheep of two experimental and control groups was performed. It was found that in all the sheep of the experimental groups, the clinical signs of estrosis (sneezing, snorting, shaking the head, rubbing the head against the legs and hard objects, excretion of serous-mucous exudate from the nostrils, sometimes with blood streaks, shortness of breath, impaired coordination of movement) disappeared, breathing recovered, general condition improved. When slaughtering two sheep — one from each experimental group — and opening the nasal passages of the larvae of the nasopharyngeal gadfly Oestrus ovis were not detected.

In sheep of the control group, clinical signs of estrosis persisted throughout the experiment. After the experiment, all the sheep of the control group with a diagnosis of estrosis were administered the claimed medicinal product in the recommended dose.

Based on the foregoing, we can conclude that the claimed preparation in a dose of 1 ml per 20 kg of animal weight, applied individually intramuscularly or when grouped in a mixture with half a single norm of concentrated feed, showed a high (EE = 100%) therapeutic effect with estrosis of sheep.

Side effects and complications when using the drug in a therapeutic dose have not been reported.

Example 17

Production experience in testing the claimed drug in injection form was carried out on 10 kids. The drug was used at a dose of 0.7 ml / 10 kg of live weight subcutaneously. Before the introduction of the drug, feces were taken from all the kids for coprological studies and it was found that out of 10 kids, 8 goals (EI = 80%) were invaded by moniesia, and the goat intestines were invaded by 100% of the intestinal tract with an intensity of invasion (AI) of 13 eggs in Suspend 3 drops.

After deworming, the effectiveness of the drug was monitored according to the results of coprological studies and it was found that the claimed drug showed 100% effectiveness against moniesia and strong gastrointestinal tract.

After using the drug every day for 3 days, we monitored the general physiological state of the lambs and did not notice any deviations from the norm, side effects and complications after using the claimed preparation were not detected.

Example 18

Two groups of goats were formed according to the principle of analogues - experimental and control of 25 animals, spontaneously invaded by helminths.

Prior to the experiment, a coprological study of the feces of goats from the experimental and control groups revealed a high degree of damage to the goats by helminths (EI - 75%). An autopsy after the forced slaughter of 2 goats of the control group revealed fascioli, dicrocelium, dictiocaula, moniesia, strongilata of the gastrointestinal tract, habertia and larvae of teniidae with high invasion intensity. The diet for animals of all groups was the same. The goats of the experimental group with predominant liver damage by trematodes were injected subcutaneously with the drug at a dose of 1 ml per 15 kg of animal weight.

The control drug was not administered to the animals of the claimed drug and other antiparasitic drugs were not given.

During the experiment, animals grazed on a pasture. With subcutaneous administration of the claimed drug to goats of the experimental group, inflammatory processes in the area of drug administration were not observed.

According to the results of coprological studies of goats from the experimental group, the effectiveness of the claimed drug against fascioliasis, dicroceliosis, dictiocaulosis and moniesiosis was 100% at a dose of 1 ml per 15 kg of body weight.

As a result of the autopsy after a forced slaughter of goats from the experimental group, one month after the treatment, not a single specimen of fasciol and diccelia was found in the liver; nematodes and cestodes in the lungs and intestines were also not detected. In the abdominal cavity, tenuicol cysticerci on the liver and omentum died.

Thus, the test results of the claimed drug against dicroceliosis, fascioliasis, diktiokauleza, moniesiosis, haberthiosis in goats indicate its high efficiency.

When using the claimed preparation, goats in a dose of 1 ml per 15 kg of body weight, anthelmintic efficacy against trematodes, nematodes and cestodes amounted to 100%.

There was no negative effect of the claimed drug on the goat’s body during the experiment, no side effects and complications after administration of the claimed drug were found.

Example 19

A clinical study of the claimed drug in the form of a solution for injection on 22 calves was conducted. When performing an experiment on calves, we studied the effectiveness of the claimed drug for strongyloidosis and strongylitis of the gastrointestinal tract, as well as the presence or absence of side effects and complications. The effectiveness of the claimed drug was evaluated in two groups of calves (gobies) Holstein breed 4-5 months of age and 2.5-3 months of age. The first and second experimental groups included 7 and 6 animals, respectively, analogues; two control groups, respectively, 4 and 5.

During the research, attention was paid to the following indicators: general condition, body temperature (optional), appetite, physical activity, symptoms of the primary disease.

In the calves of the experimental and control groups - diarrhea (feces are liquid, in some cases with undigested food), appetite and motor activity are reduced. Two experimental animals showed signs of stunting. The weight gain in calves of the experimental and control groups is lower by 20-25% compared with animal counterparts without symptoms of the disease.

According to the results of coproovoscopic and larvoscopic studies of calves of the experimental and control groups, eggs and larval stages of nematodes from the families Strongylidae (Chabertia ovina), Trichostrongylidae (Ostertagia spp., Nematodirus spathiger), Trichonematidae (Oesophagostomumloidloid strong).

The claimed drug was used subcutaneously at a dose of 1 ml per 20 kg of animal weight. The drug in the specified dose for calves of each experimental group was used once.

For control animals, the claimed drug in the above dose was used at the end of the experiment.

In a clinical study, the terms of clinical recovery and the duration of the rehabilitation period after completion of treatment were clarified.

In the first experimental group, all calves at 5-10 days after the use of the drug observed an improvement in general condition, an increase in appetite and motor activity.

In the second experimental group of calves for 7-12 days, in addition to the above clinical indicators, the cessation of diarrhea and a gradual increase in weight gain were observed.

In laboratory studies of animals of the first and second experimental groups, 10-12 days after the use of the antiparasitic preparation, eggs and larvae of intestinal nematodes were not found. The data obtained are presented in table 4.

In the calves of the control groups, during the experiment, symptoms of gastrointestinal diseases were noted, and with coproovoscopic and larvoscopic examinations, the diagnosis of strongyloidosis and strongylitis of the gastrointestinal tract was confirmed.

As a result of the studies, it was found that the terms of the clinical recovery of the calves of the experimental groups after applying the claimed drug are 5-12 days, and the full rehabilitation period is 15-17 days. Based on the results of epizootological monitoring, the optimal terms for treatment and preventive measures for mixed forms of intestinal nematodoses of young cattle in a large dairy complex were determined.

Side effects of the claimed drug according to the results of the experiment has not been established.

Thus, the claimed preparation at a dose of 1 ml per 20 kg of animal weight, administered subcutaneously, has a pronounced nematodicidal effect, is effective for larval and imaginal forms of strongyloidosis and strongylatosis of the gastrointestinal tract of young cattle. In the treatment of enterocolitis of nematode etiology, the period of clinical recovery of calves is 5-12 days, and the duration of the rehabilitation period is 15-17 days.

Example 20

In a dysfunctional helminthiasis of ruminant animals, 2 experimental groups of calves of 20 goals were formed and 1 control group of calves of 20 goals. An experimental group of calves No. 1 was administered the claimed preparation once intramuscularly with trematodoses at a dose of 1 ml per 15 kg of animal weight. An experimental group of calves No. 2 was administered the claimed drug with cestodoses once intramuscularly at a dose of 1 ml per 20 kg of animal weight. A study of feces of calves by flotation.

Control animals received no antiparasitic drugs.

During the experiment, the young cattle were kept on the farm loose with access to walking areas. A coprological examination of the feces of calves from the experimental and control groups revealed a 68% helminth lesion of the calves. The diet for animals of all groups was the same. The body weight of young cattle during the experiment was from 60 to 150 kg.

With the intramuscular administration of the claimed drug to experimental animals, no inflammatory processes were observed. It is convenient to administer the claimed preparation in the form of an injection to animals. After administration of the drug for 3 days, the state of animal health was monitored.

The effectiveness of the claimed drug was evaluated according to the results of a study of samples of feces of calves of the experimental groups by flotation before and 18 days after administration of the drug. According to the results of a coprological study of the feces of the calves of the first experimental group treated with the claimed drug in a dose of 1 ml per 15 kg of body weight, the efficacy against trematodes was 96%.

According to the results of coprological studies of feces of calves of the second experimental group, the effectiveness of the claimed drug against cestodes in a dose of 1 ml per 20 kg of animal weight was 100%.

All the calves of the experimental groups after treatment with the claimed preparation were released from the strongilata of the digestive tract. Extensibility was 100% at a dose of 1 ml per 15 kg of body weight and at a dose of 1 ml per 20 kg of body weight. In animals of the control group, the extensinvasion by nematodes remained unchanged. If the initial average number of nematode eggs in 1 g of feces was 185 ± 14.2 specimens, then after 18 days it was 193 ± 14.5 specimens.

When deworming animals of the experimental groups of the claimed drug, adverse reactions at the injection site were not noted. The general condition of the animals of the experimental groups is within the physiological norm.

Fragments were strobed and individual segments of cestodes of the genus Moniezia were absent in the feces of animals from the experimental groups.

Thus, the dose of the claimed drug - 1 ml per 15 kg of animal weight - at which the intensity was obtained - 100% for moniesiosis and nematodoses and 98% for calves trematodoses - is recommended as therapeutic.

The results of studies of the anthelmintic efficacy of the claimed drug give reason to recommend this drug in veterinary practice for the treatment and prevention of moniesiosis and trematodiasis in cattle.

The negative effect of the claimed drug on the animal organism during the study was not noted.

Example 21

Under the experience were 20 heads of young cattle.

Of calves 6-7 months of age of black-motley breed weighing 180-200 kg with clinical signs of entomosis, characterized by anxiety, itching, scratching, ruffled coat, the presence of dermatitis and bald patches in the ears, underbelly and tail, two groups were formed - experimental and control. The calves grazed in the pasture during the day, at night the calves were indoors. Feeding the calves was carried out in accordance with the standards for this species of animals, water without restrictions.

The diagnosis was made when a mallophage was detected on the body of animals in different phases of development. When examining the animals at the beginning of the experiment, it was found that all of them were attacked by Bovicola bovis with an invasion intensity of 100% and an intensity of 5.7 to 19.3 ind. over an area of 10 cm. Foci with eggs of the lice on the basal part of the hair were also found.

Differential diagnosis was carried out in order to exclude the defeat of animals by sarcoptoid mites: in the skin scrapings taken from the lesion sites, pathogens of cutaneous and itchy scabies were not found.

The calves of the experimental group were injected once intramuscularly the claimed drug in a dose of 1 ml per 20 kg of animal weight.

Two weeks later, the calves of the experimental group were re-injected intramuscularly the claimed drug at a dose of 1 ml per 20 kg of animal weight. Individual doses of the drug are shown in table 5.

The control group of calves claimed drug was not administered.

The general condition of the calves was monitored throughout the experiment.

Evaluation of the effectiveness of the drug was carried out on the basis of changes and disappearance of the clinical signs of the disease and taking into account the number of water-eaters by visual inspection and counting their number in three different parts of the body with an area of 10 cm ² 2 weeks after repeated administration of the drug.

When examining the calves of the experimental and control groups two weeks after treatment, the following was established: in calves of the experimental group who were injected with the claimed drug intramuscularly at a dose of 1 ml of the drug per 20 kg of animal weight twice with an interval of 14 days, anxiety and itching were gone, scratching stopped, In the areas of bald patches and bald spots in the ears, chest and tail, inflammations took place and hair growth began. The general condition of the calves has improved significantly. The coat is smooth, shiny, and there are no water-eaters and eggs on the skin-hair cover.

The therapeutic efficacy of the claimed drug for boviculosis was 100%. All calves of the experimental group were freed from Bovicola bovis. Side effects and complications, adverse reactions after administration of the drug were not identified.

The results of accounting for infection of calves Bovicola bovis experimental and control groups before and after treatment with the claimed drug are presented in table 6.

The calves of the control group who did not receive treatment retained the clinical signs of bovikolosis: itching, anxiety, scratching of the pathogen localization sites, focal dermatitis, allopecia, hair was ruffled, dull, Bovicola bovis and their eggs were found on the skin and hairline, and appetite was reduced. The number of water-eaters per unit surface area of the body increased by the end of the experiment compared with the number of water-eaters per unit area before the start of the experiment.

Thus, as a result of the studies, it was found that the claimed drug in a dose of 1 ml per 20 kg of animal weight, administered individually intramuscularly twice with an interval of 10-14 days, is an effective drug against boviculosis of cattle. Its effectiveness in cattle bovycoliasis is 100%.

Side effects, complications, adverse reactions after administration of the claimed drug are absent.

After completion of the study, animals of the control group were also treated with the claimed drug in the recommended dose and according to the recommended schedule.

Example 22

Reindeer in the amount of 40 animals were divided into two experimental groups: 1 experimental group - calves 4-5 months (20 goals), 2 experimental group - gobies, heifers 16-17 months (20 goals),

Before the introduction of the drug, feces were taken from all animals for coprological studies and it was found that all 40 deer were infected with helminths, including: moniesia - 24 goals (EI - 60%), with invasion intensity (II - 23.7 ind./ goal) , ostertagia - 36 goals (EI -90%, II - 23.5 ind./ goal), nematodiruses - 20 goal (EI-50%, II - 20.3 ind./ goal).

The claimed drug was administered to animals intramuscularly at a dose of 1 ml per 20 kg of live weight.

Observations of the clinical condition of the animals over 3 days did not reveal differences from the normal physiological state of the animals. At the injection site, an insignificant inflammatory reaction was noted in three animals, which independently passed within 3 days.

After 10 days, we took into account the effectiveness of the drug according to the results of coprological studies and found that the claimed drug showed 100% effectiveness against moniesia, ostertagia and namatodiruses (see table. 7).

As follows from the data of table 7, the claimed drug when administered once intramuscularly at a dose of 1 ml per 20 kg of animal weight, showed 100% antiparasitic efficacy in deworming of reindeer.

Example 23

From 40 red deer, an experimental and control group was formed. The weight of the animals of the experimental and control groups ranged from 90 to 230 kg. All deer of the experimental group — 30 goals and 10 goals of the deer of the control group were spontaneously infected with helminths and arachno-entomoses.

During the experiment, all animals were grazed in the same conditions. The diet for animals of all groups was the same.

A co-ovoscopic method for studying the deer feces of the experimental and control groups, carried out before the start of the experiment, established 100% deer helminths (EI 100%) at the time the experiment began.

Before the start of the experiment, 13 deer of the experimental group and 3 deer of the control group had lesions on the sides of the body, in the back and sacrum, calculations, and itching. At the lesion sites, nodules, papules, and crusts were noted on the skin, and ticks of the Sarcoptidae family were found in skin scrapings. In 6 deer of the experimental group and 2 deer of the control group, subcutaneous larvae were found in the subcutaneous tissue of the back ^; gadfly family Hypodermatidae. On the body and in the hair of 23 deer of the experimental and 5 deer of the control group, the bloodsucker Lipoptena cervi was found. At the same time, the deer were very worried, itchy, itched on the trees. 11 deer were diagnosed with dermatitis.

All animals of the experimental group were injected with the claimed drug intramuscularly at a dose of 1 ml per 20 kg of live weight.

Animals of the control group were not treated with the claimed drug, and other antiparasitic drugs were not given to them.

The claimed drug showed 100% effectiveness with nematodoses of the digestive tract, with nematodoses of the lungs, with cestodoses of deer.

After 14 days, the experimental group of deer was re-treated with a diagnosis of sarcoptosis, hypodermatosis and lipoptenosis of the claimed preparation in the above dose.

The effectiveness of the claimed drug was evaluated by the presence, subsidence or absence of clinical signs of sarcoptosis, microscopy of scrapings from skin lesions (on the border with a healthy area) before and 28 days after treatment of deer of the experimental group compared with the control group. Using a microscope, the number, stages of development of ticks and their condition were determined. The presence or absence on the body of animals of the larvae of the subcutaneous gadfly and bloodsucker was visually determined.

The claimed drug showed 100% effectiveness in nematodoses, cestodoses, sarcoptosis of deer with varying degrees of damage in case of hypodermatosis and lipoptenosis.

Treated animals on the affected areas of the skin began to appear hair, signs of inflammation disappeared. With a further study of scrapings of the skin of the deer of the experimental group, 48 hours after double treatment with the claimed preparation, no ticks were found. The number of ticks of the Sarcoptidae family in the deer of the control group remained the same.

Received 100% effectiveness of the claimed drug when applied twice with an interval of 14 days for hypodermatosis and lipoptenosis of deer.

The absence of experimental groups of bloodsuckers and larvae of the saphenous gadfly on the deer persisted for three months. When examining the deer of the experimental group after treatment, no dermatitis, scratching and anxiety were recorded.

Side effects, complications, adverse reactions from the deer after the introduction of the claimed drug was not detected.

The invasion of deer in the control group during the experiment did not change significantly.

After the study, the animals of the control group were treated with the claimed drug in the above dose and according to the above scheme.

Example 24

At a private compound, a clinical examination of the chicken population in the amount of 28 animals was conducted. The age of chickens is from 1 year to 1.5 years. Upon examination, it was found: the bird is exhausted, the feather is tousled, there are places of skin peeling and feather falling out. According to the owner, the poultry egg production decreased, but feed consumption increased, the bird worries, constantly sorting feathers on itself.

On closer inspection, small parasites about 1 millimeter in size, red in color, quickly moving through the body of the bird, were found on the body of the hens. This parasite has been identified as a chicken tick (Dermanyssus gallinae). We also examined the chicken coop for the presence of a tick in the slit spaces of the walls. A sheet of white paper was substituted for the slot and the contents of the slot were swept out with a goose pen. At the same time, small red parasites about 1 millimeter in size, which were also identified as a chicken tick (Dermanyssus gallinae), fell on a paper sheet.

An experimental group of birds (23 heads) was treated with the claimed drug in a dose of 1 ml of the drug per 20 kg of bird weight once, in a group way with water, in the morning on an empty stomach. Before processing, the total weight of the hens of the experimental group was determined. Based on the mass, the amount of the drug per group was determined based on 1 ml of the drug per 20 kg of chicken mass. A single amount of water per group was determined based on 50 ml of water per head. Heated water to 35 ° C for better dissolution of the drug. We mixed a measured amount of the claimed drug with a measured amount of water to obtain a working solution of the drug, poured it into a clean drinker and put the drinker in the aviary with an experimental group of chickens. The bird of the control group was not allowed to enter the water with the preparation. After processing the claimed drug, the hens of the experimental group were left for a day in the aviary to monitor their clinical condition. Feeding was not limited. Throughout the daylight hours after treatment and for the next two weeks, according to our observations and the observation of the owner of the bird, no negative changes in the condition and behavior of the treated bird were noted. According to the owner’s observations, on the 3rd day after treatment, the appetite and general condition of the poultry treated with the claimed preparation improved, and egg production increased. The control group remained the same clinical signs that were observed in the entire poultry population before treatment with the claimed drug.

Two weeks later, to determine the effectiveness of the treatment with the claimed drug, a repeated clinical examination of the treated bird was performed. No ectoparasites were found on the body of a bird from an experimental group. In the control group, chicken tick (Dermanyssus gallinae) was observed on the body.

On the same day, the treatment of the birds of the control group was carried out, as well as the repeated treatment of the birds of the experimental group for the purpose of prevention, by the claimed preparation in a group way with water at the rate of 1 ml per 20 kg of bird weight according to the same principle as before. After reprocessing, no negative changes in the state and behavior of the bird were observed.

Thus, the effectiveness of the claimed drug in a dose of 1 ml per 20 kg of bird mass was once orally grouped with water, in the morning on an empty stomach, with chicken dermanissiosis, it was 100%. Side effects of the drug were not found.

Example 25

About 10 heads of outbred chickens aged 5 months to 2 years participated in the experiment. A clinical examination of chickens was conducted. Chickens of average fatness weighing from 1.5 to 3 kg. In some chickens, emaciation, lethargy, lack of mobility, poor appetite, taunting, drooping of the head and wings, diarrhea, thinning of the feather-feather cover, and picking of feathers with beak were noted. 10 chickens were examined for the presence of ectoparasites - all were found to have lacunae and spoofers (EI - 100%). Group samples of freshly excreted feces were taken from the earthen floor of the enclosure for laboratory testing for helminthiasis.

In a laboratory study of group samples of chicken feces by inspection, sequential washing and flotation according to Fulleborn, eggs of Heteracis gallinarum heterekis, Thominx collaris capillary, Ascaridia galli ascaridia, and ryllietin segments Raillietina tetragona were found. Lepidoptera have been identified as Menopon gallinae, lacunae as Lipeurus caponis

After the inspection, deworming of the whole livestock was carried out with the claimed preparation in a dose of 1 ml per 20 kg of live weight of chickens once orally with water in a group manner on an empty stomach. Pre-determined the total weight of the group of chickens - the average weight of the chicken 2.5 kg × 100 = 250 kg. Then determined the required amount of water per group - 30 ml × 100 = 3 liters. Using a syringe, 12.5 ml of the claimed preparation was measured and mixed with 3 l of water heated to 30 ° C. The resulting solution was poured into an empty drinker. Chickens drank the entire solution in 1.5 hours. After that, they poured water into the drinker and gave the chickens food.

After deworming, the chickens showed slight excitement and excretion of Ascaridia galli, heads and fragments with a litter of Ascaridia galli, and the cestode, ryllietin Raillietina tetragona, strobe. There are no clinical signs of the negative effect of the drug on chickens. According to the observations of the owner of the hens, the general condition of the hens improved significantly the very next day - the appetite returned to normal, lethargy, tearing, drooping of the head and wings went away. Two weeks after the administration of the drug, when examining the hens, the clinical signs noted earlier were not observed, the condition of the hens improved significantly, and the feather cover improved. When examined for ectoparasites, it was found that the number of pohoedov and pooedov significantly decreased. In a laboratory study of 3 group samples of hen feces, taken two weeks after the hens were treated with the claimed preparation, eggs and segments of helminths were not detected.

Thus, experiments carried out on chickens spontaneously invaded by Heteracis gallinarum heterekisis, Thominx collaris capillaries, Ascaridia galli ascaridia, Raillietina tetragona eryllietins, Menopon gallinae poo-eaters, and a live dose of 20 ml the mass of chickens once orally with water in a group manner in the morning on an empty stomach, has a pronounced effect in case of nematodoses - heterecidosis (EE-100%), capillariosis (EE - 100%), ascariasis (EE-100%), cestodoses-ryllietinosis (EE - 100%) , as well as pohoyoedov and peroedov. In the specified dose, the claimed drug does not have a negative effect on chickens.

Example 26

The experiment involved 23 heads of geese, of which 5 geese weighing 6-7 kg, 1 gander weighing 7 kg and 17 goslings this year weighing 1-1.5 kg.

When conducting a clinical examination of geese, the following was established: the state of 5 geese and a gander without features - gray-green droppings liquid with mucus; this year’s goslings there is depression, inactivity, emaciation, diarrhea, feces of gray-green and gray-brown color with an admixture of mucus and segments of cestodes (segments of white color from 4 to 10 mm wide); some goslings noted curvature of the neck, tilting or drooping of the head, shakiness, lack of coordination during movement, frequent opening of the beak, mucus secretion from the beak, "croaking". According to the owner of the geese, over the past month 11 goslings fell, the remaining goslings eat poorly, but drink water willingly (thirst).

In the study of fecal samples taken in the laboratory using inspection methods, Fulleborn flotation and sequential washing, echinostomatides and their eggs, segments of drepanidotenia, hymenolepidids and their eggs, ganguleteracis eggs, amidostom and capillary, Eimeria truncata oocysts were found. The extensinvasion of young animals was: for echinostomatidoses - 47%, drepanidoteniosis - 100%, hymenolepididosis - 76.5%, ganguleteracidosis - 70.6%, amidostomiasis - 82.3%, capillariosis - 58.8%, eimeriosis - 70.6%. Extensinvasion of the uterine population amounted to: 16.6% for echinostomatidoses, 33.6% for drepanidoteniosis, 33.3% for hymenolepididoses, 66.6% for ganguletheracidosis, 83.3% for amidostomiasis, and 88.2% for capillariosis.

At the autopsy of a caterpillar that fell on the day of the study, amidostomas were found in its muscular stomach, drepanidotenia, hymenolepis, echinostomatida and capillary in the small intestine, and ganguleteracis in the cecum.

We performed deworming of the entire livestock with the claimed preparation in a dose of 1 ml of the preparation per 20 kg of geese mass once orally with water in a group manner on an empty stomach. Pre-determined the total weight of the group of geese, it amounted to 67.5 kg. Based on the mass of the geese group and the dose of the claimed preparation, the required amount of the preparation was determined - 3.4 ml. Next, the amount of water per group was determined - 50 ml was multiplied by 23, 1150 ml were obtained. Using a syringe, 3.4 ml of the claimed preparation was measured and mixed with 1150 ml of water. The resulting solution of the drug was poured into an empty drinker and geese were let in. The geese drank the entire solution within 15 minutes (previously the geese had not been drunk or fed since morning). After that, water was poured into the drinker, food was not given. Deworming was carried out in the morning. After deworming, geese were observed for 3 hours, and the owner of geese was monitored for the rest of the day and daily.

After 15-20 minutes after the preparation was drunk, the geese became excited - they began to walk and gaggle more actively. After 35-40 minutes, active defecation and discharge of cestodes (drepanidotenia and hymenolepidide) and trematodes (echinostomatids) with litter began. Active defecation lasted 15-20 minutes, after which the excitement ceased. Watering during the observation was not limited, food was not given for three hours of observation, after which the geese were released into the meadow. By evening, according to the owner, the state of the geese has improved significantly. In the following days, appetite returned to normal, geese became more active, deaths stopped. Side effects, complications, adverse reactions to the claimed drug from the bird were not recorded.

During a clinical examination of deworming geese, the clinical signs noted earlier were not observed. The general condition of the geese was in line with the norm. There was no deaths during the study period. In a laboratory study of fecal samples taken a week after deworming, segments and helminth eggs were not found.

When viewed two weeks after the deworming of geese with the claimed preparation, no clinical signs of helminthiasis were detected. Geese are active, appetite is good, young growth and normal development. There have been no cases over the past period. All geese took individual feces samples.

In a subsequent laboratory study of fecal samples taken two weeks after deworming, eggs and segments of helminths were not detected.

When examining geese, carried out 3 weeks after deworming, no clinical signs of helminthiasis were detected. The state and appetite of the geese are good. Young growth and normal growth. There was no case for the past period. All geese took individual feces samples. Samples were examined in the laboratory using inspection methods, Fulleborn flotation and sequential washing to detect segments and helminth eggs. In samples of the uterine livestock, segments and helminth eggs were not detected. In 5 samples from young animals, single segments of Drepanidothenia were found, in 4 samples - single segments of hymenolepidids, in 7 samples - eggs of trematode-echinostomatids, in 10 samples - Eimeria oocysts. Eggs of nematodes in samples of young and brood stock were not identified.

The presence of single segments of drepanidotenia, hymenolepidids, and echinostomatid eggs in fecal samples obtained three weeks after deworming, taking into account their absence in samples obtained one week and two weeks after deworming, indicates reinvasion, i.e. re-infection of geese with drepanidoteniosis, hymenolepidosis and echinostomiasis. The absence of nematode eggs in the samples obtained three weeks after deworming indicates the protective effect of ivermectin, which is part of the claimed means, against gem nematodes.

Based on tests of the anthelmintic efficacy of the claimed drug in the form of a solution for three weeks on 23 geese spontaneously invaded by trematodes (family Echinostomatidae), cestodes (family Hymenolepididae), nematodes (family Heterakidae, Amidostomatidae, Capillariidae) :

- the claimed drug, administered orally at a dose of 1 ml per 20 kg of geese once with water in a group manner in the morning on an empty stomach, has 100% extensibility and intensification with tremato doses (echinostomatidoses), cestodoses (drepanidoteniosis, hymenolepidosis), nematodoses (amidostetosis, ganga capillariosis);

- in the specified dose, the claimed drug has a long, at least three weeks, protective effect against nematodes (amidostom, ganguleteracis, capillary);

- the claimed drug mixes well with water, forming a single working solution, as a result of which it can be used to deworm birds in a group way with water freely;

- the method of use is convenient, effortless, eliminates the stress of the bird;

- the claimed preparation in a single dose of 1 ml per 20 kg of bird weight when administered orally with water does not have a clinically pronounced negative effect on geese. Side effects, complications, adverse reactions when using the claimed drug were not noted.

Example 27

The experiment involved 30 heads of turkeys, of which 15 turkeys weighing 4.8-5.2 kg, 3 turkeys weighing 5.8-6.1 kg and 12 turkey poults of this year of birth weighing 2.0-2.5 kg.

A clinical examination found: litter in turkeys and turkeys is gray-green, liquid with mucus; sedentary turkey poultry, emaciated, feces liquid gray-green and gray-brown in color mixed with mucus and segments of cestodes; some turkeys have curvature of the neck, tilting or drooping of the head, unsteady gait, impaired coordination of movement, frequent opening of the beak, some of the beak have mucous secretions, and appetite is reduced.

When opening a fallen turkey in the lumen of his intestines, a large amount of mucus, pinpoint hemorrhages; in the small intestine - pressure, in the cecum - heterokisy, tominx, in the factory bag - simple homonyms.

In the study of fecal samples taken by examination, Fulleborn flotation and sequential washing, nematode eggs of the syngamide family, heterokisis, tominxoses, pressure segments, raietinia and their eggs, eggs of simple homonyms were found.

Extensinvasion of young animals was: by protogonymoses - 42%, daveniasis - 80%, rallytinosis - 71.5%, heterocycosis - 68.3%), tominxosis - 51.5%. Extensinvasion of the uterine population amounted to: according to protogonymoses - 11.2%, daveniasis - 28.3%, rallytinosis - 35.5%, heterocycosis - 63.4%, syngamosis - 28.1%.

We performed deworming of the entire livestock with the claimed drug in a dose of 1 ml of the drug per 20 kg of live weight of turkeys orally once with drinking water in a group way before feeding (on an empty stomach). A preliminary estimated amount of the claimed drug was mixed with drinking water. The resulting solution of the drug was poured into an empty drinker and turkeys were let in. The turkeys drank the entire solution within 15 minutes. In the morning, turkeys were not watered or fed. After that, water was poured into the drinker, they did not give food. After deworming, the turkeys were observed for three hours, the owner of the turkeys - until the end of the day and then every day.

After 35-40 minutes, active defecation and cestode discharge (pressure of meleagris, erythrocytes) with litter began. An individual sample of freshly excreted feces was taken from each bird from the ground. Active defecation lasted 15-20 minutes. Watering during the observation was not limited, food was not given for three hours of observation. By evening, according to the owner, the condition of the turkeys has improved significantly. In the following days, appetite returned to normal, turkeys began to behave more actively, deaths stopped. Side effects, adverse reactions and complications after using the claimed drug in turkeys were not observed.

In a laboratory study of fecal samples taken a week after helminthization, segments and helminth eggs were not found.

Two weeks after deworming conducted a re-examination of the turkeys. There are no clinical signs of helminthiases. The bird is active, the appetite is good, the young growth and normal development. There have been no cases over the past period. Individual turkey samples were taken from all turkeys. No eggs and segments of helminths were found.

3 weeks after deworming of turkeys with the claimed preparation, they did not reveal any clinical signs of helminthiases. The condition and appetite of the bird is good. There is no case for the past period. Individual turkey samples were taken from all turkeys. Samples were examined by inspection methods, Fulleborn flotation, and sequential washing to detect segments and helminth eggs. In samples of the uterine livestock, segments and helminth eggs were not detected. In 2 samples from young animals, single segments of ryllietin were found, in 3 samples - single segments of pressure, in 5 samples - eggs of trematodes - protogonim. Eggs of nematodes in samples of young and brood stock were not identified.

The presence of single segments of cestodes and eggs is simply taken in feces samples three weeks after helminthization of turkeys, taking into account their absence in samples taken two weeks after helminthization, indicates reinfestation, i.e. reinfection of turkeys. The absence of nematode eggs in the samples taken three weeks after deworming indicates the protective effect of ivermectin, which is part of the claimed preparation, against turkey nematodes.

Based on the data obtained, we can conclude that:

- the claimed drug in a dose of 1 ml per 20 kg of live weight of turkeys, when administered orally once a group of water in the morning on an empty stomach, has 100% extensi- and intensivity in trematodoses, cestodoses, turkey nematodes;

- in the specified dose, the drug has a long, at least three weeks, protective effect against turkey nematodes;

- the claimed preparation mixes well with water, forming a solution that can be used for deworming of birds in a group way with water;

- the method is convenient, easy and eliminates the stress of the bird;

- when using turkeys of the claimed drug in a dose of 1 ml per 20 kg of live weight once with water for drinking side effects and complications have not been established.

Example 28

Clinical studies of the claimed drug were conducted on 36 spontaneously infested helminthiases ducks. According to the owner, on the eve of the study on the farm, four ducks fell. When opening the dead ducks in their intestines, tangles of ribbon-shaped helminths were found 10-15 cm long and up to 3 mm wide.

As a result of the clinical examination, the following was established: in most ducks there is lethargy, weakness, emaciation, in some - depression, unsteadiness of gait, contamination of the cloaca area by droppings.

According to the principle of analogues, 2 groups were formed - experimental (26 goals) and control (10 goals). Ducks of the control group after ringing were released into a spacious enclosure, ducks of the experimental group into the yard.

When helminthoscopy, flotation, and sequentially washing fecal samples taken from all ducks, echinostomatids (Echinostoma revolutum, Hypodereum conoideum) and their eggs, strobiles and fragments of strobil heminolepidide (Hymenolepis setigera, H. gracilis), and their eggs were found ganguleterakis (Ganguleterakis dispar), streptocar, tetrameres, echinuria. The extinction invasion of ducks in the experimental group was 35% for echinostomatidoses, 50% for hymenolepididoses, 31% for ganguletheracidosis, 23% for streptocarosis, 19% for tetramerosis, and 15% for echinuriosis. The extinction invasion of the ducks in the control group was 30% for echinostomatidoses, 40% for hymenolepididoses, 40% for gangletheracidosis, 30% for streptocarosis, 20% for tetramerosis, and 20% for echinuriosis.

All ducks of the experimental group were dewormed with the claimed drug, which was administered orally at a dose of 1 ml of the drug per 20 kg of duck body weight once with water in a fasting group manner. The solution of the drug was poured into a clean drinker and let the ducks of the experimental group. The ducks drank the entire solution within 15 minutes. After that, pure water was poured into the drinker, and no food was given. The ducks of the control group were given water without a drug.

15-20 minutes after drinking the claimed preparation, the ducks of the experimental group began to move and quack more actively. After 30 - 40 minutes, active defecation and discharge of trematodes (echinostomatids) and cestodes (hymenoleptidids) with litter began. In the process of defecation, an individual sample of freshly excreted feces was taken from the ground from each bird. Active defecation lasted 15-20 minutes, after which the excitement ceased.

Fecal samples were collected from ducks in the control group in a similar manner. The ducks of the experimental and control groups were observed for 3 hours, then all the ducks were released into the meadow. Negative reactions to the claimed drug in ducks of the experimental group for 3 hours of observation and in the subsequent period until the end of the experiment were not. According to the observations of the owner of the ducks, in 8-10 hours after deworming, the state of the ducks of the experimental group improved significantly. On examination the next day and in the subsequent period, the normalization of the state of the ducks of the experimental group was noted.

Positive dynamics in the state of the ducks of the control group until the end of the experiment was not observed, their condition worsened, two heads fell.

A clinical examination of the ducks of the experimental group, carried out 10 days after deworming, showed a significant improvement in their condition - normal appetite, increased activity, stopped diarrhea.

Positive changes in the state of the ducks in the control group were not noted.

Individual feces were collected from all ducks.

In a laboratory study in the samples of duck feces of the experimental group, segments and helminth eggs were not detected, which indicates 100% extensibility and intensification of the claimed drug in the tested dose for trematodoses, cestodoses and nematodes.

Extensibility of ducks in the control group during the week increased and amounted to: echinostomatidoses 30%, hymenolepididoses 50%, ganguletericidosis 40%, streptocarosis 40%, tetramerosis 50%, echinuriosis 20%. Two ducks of the control group fell.

The fallen ducks were opened. It is established that the corpses are exhausted. The internal organs of the thoracic and abdominal cavity were examined by the method of complete helminthological autopsy according to K.I. Scriabin. In the small intestine of both birds, balls of cestodes of the family Hymenolepididae Hymenolepis setigera, H. gracilis, and trematodes of the family Echinostomatidae Echinostoma revolutum, Hypodereum conoideum were found. The mucous membrane of the small intestine is hyperemic with numerous point hemorrhages, clots of clotted blood in the intestinal cavity. The liver is enlarged, red-brown with gray spots. In addition to hymenolepis and echinostomatids, in one bird, Tetrameres flssispina tetramereses were found in the glandular stomach, Streptocara crassicauda streptocar in the muscular stomach, and Ganguleterakis dispar in the cecum of the ganglion; in the other, in the glandular stomach, Echinuria uncinata echinuria, Streptocara crassicauda streptocar in the muscular stomach, and Ganguleterakis dispar in the cecum of the gangs. Thus, the cause of death of both ducks was a mixed invasion of helminths.

14 days after deworming during the examination of the ducks of the experimental group of clinical signs of helminthiasis was not found.

The clinical condition of 8 ducks of the control group worsened - the birds were exhausted, lethargic, gait shaky, the area of cloaca in all was stained with droppings, in the litter on the ground there were segments of hymenolepids.

All ducks of the control and experimental groups were dewormed with the claimed preparation at a dose of 1 ml per 20 kg of body weight. A measured dose of the drug was mixed with 1.5 liters. water and drunk freely in a group way in the morning on an empty stomach.

After 30 minutes, the ducks in the control group started to leave with a litter of red trematodes about 1 cm long and 2-3 mm wide (echinostomatide) and strobed the cestode up to 15 cm long and up to 3 mm wide (hymenolepidide).

Individual birds of feces were taken from all birds of the experimental and control groups.

When feces samples were studied using helminthoscopy, flotation and sequential washing in ducks of the control group, echinostomatids (Echinostoma revolutum, Hypodereum conoideum) and their eggs, strobiles and fragments of strobil heminolepid (Hymenolepis setigera, H. gracilis), and egg eggs, were found (Ganguleterakis dispar), streptocar, tetrameres, echinuria. The extinction invasion of ducks in the control group was 37% for echinostomatidoses, 75% for hymenolepididoses, 50% for ganguletheracidosis, 37% for streptocarosis, 50% for tetramerosis, and 37% for echinuriosis.

In the samples of feces of ducks of the experimental group (26 animals) that were initially deworming two weeks earlier, eggs of nematodes (ganguleteracis, tetrameres, streptocar, echinuria, etc.) were not found. Trematodes, echinostomatids and their eggs, were found in three samples, and immature echinostomatids in two samples (EI 19%); in six samples, cestode gimenolepidide was detected at the preimaginal stage (EI - 23%).

The absence of nematode eggs in all feces samples taken a week after deworming in ducks of the experimental group indicates the protective effect of ivermectin, which is part of the claimed drug. The presence of echinostomatids in 19% of samples and preimaginal forms of hymenolepidids in 23% of samples obtained two weeks after deworming indicates a reinfestation, i.e. re-infection of ducks with hymenolepididoses and echinostomatidoses.

Thus, the anthelmintic test results

the effectiveness of the claimed drug in the form of a solution on 36 ducks, spontaneously invaded by trematodes (ganguleteracis, streptocara, tetrameris, echinuria), allows us to draw the following conclusions:

- the claimed drug, applied at a dose of 1 ml per 20 kg of live weight of ducks once orally with water in a group manner in the morning on an empty stomach, has 100% extensibility and intensification with trematodoses (echinostomatidoses), cestodoses (hymenolepididoses), nematodoses (ganguleletiracosis, streptocarosis, tetramerosis, echinuriasis);

- in the specified dose, the claimed drug has a long-lasting - at least two weeks-protective effect against nematodes (ganguleteracis, streptocar, tetrameres, echinuria);

- the claimed preparation mixes well with water, forming a uniform working solution, as a result of which it can be used for deworming of birds in a group way with water;

- the method is convenient, effortless, eliminates the stress of the bird;

- the claimed drug in a dose of 1 ml per 20 kg of live weight of the bird, used orally in a group manner with water, does not have a clinically pronounced negative effect on ducks. After the use of the claimed drug in the indicated dose, no adverse reactions were detected in the bird, no side effects and complications.

Based on the foregoing, we can conclude that the declared:

- the method is effective for a wide range of endoparasitic diseases - for cestodoses, nematodoses of the gastrointestinal tract and lungs, trematodoses - for a wide range of artiodactyls and equidae and poultry, as well as for ectoparasites of farm animals and poultry - estrosis, ixodidosis, melophagosis psoroptosis, sarcoptosis;

- the drug can be administered either in the form of an injection or individually orally, while there is no inflammatory reaction when it is administered as an injection at the injection site;

- the drug mixes well with both water and feed, so it is possible to use it for farm birds or small cattle in a group way with drinking water or with feed mixture, which facilitates helminthization;

the drug is well tolerated by animals and poultry, with its use in the recommended doses of side effects, complications and adverse reactions have not been established;

- the drug is stable during storage.

Claims (2)

1. A method for the prevention and treatment of parasitic diseases of farm animals and birds, characterized in that the animals and birds, when they are injured by ecto and / or endoparasites, are injected once or twice subcutaneously, or intramuscularly, or orally in a mixture with concentrated food or with water, containing in g per 100 ml: ivermectin - 0.4-0.6, praziquantel - 5.0-7.0, methylpyrrolidone - 24.0-26.0, benzyl alcohol - 0.5-1.5, propylene glycol - up to 100, at a dose of 0.6-1.2 ml per 15-20 kg of live weight. 2. A method for the prevention and treatment of parasitic diseases of farm animals and farm birds according to claim 1, characterized in that the drug is administered orally in a mixture with concentrated feed or with water individually or in a group manner.