RU2784353C2 - Nutritional mixture for achievement of healthy effects in aminals - Google Patents

Abstract

FIELD: pharmaceutical industry; veterinary medicine.

SUBSTANCE: group of inventions relates to the pharmaceutical industry, namely to veterinary medicine, to a composition for the treatment or prevention of breast cancer in an animal, and a method for the treatment or prevention of breast cancer in an animal. The method for the treatment or prevention of breast cancer in an animal includes oral administration to the animal of a composition containing 11.3 wt.% of whole walnut flour, 0.005 wt.% of maitake mushroom extract, 0.05 wt.% of epigallocatechin gallate (hereinafter – EGCG), 3 wt.% of turmeric root powder, 0.005 wt.% of lycopene, 0.1 wt.% of taurine, and 2 wt.% of fish oil. The composition for the treatment or prevention of breast cancer in an animal contains 11.3 wt.% of whole walnut flour, 0.005 wt.% of maitake mushroom extract, 0.05 wt.% of EGCG, 3 wt.% of turmeric root powder, 0.005 wt.% of lycopene, 0.1 wt.% of taurine, and 2 wt.% of fish oil.

EFFECT: above-described composition is effective for the treatment or prevention of breast cancer in an animal.

11 cl, 3 tbl, 1 ex

Description

Cross-reference to related applications

The present application claims priority over U.S. Provisional Application No. 62/583,830, filed Nov. 9, 2017, the disclosure of which is incorporated herein by this reference.

Prerequisites for the creation of the invention

One billion people do not have access to health systems. In 2015, of the 56.4 million deaths worldwide, more than half (54%) were due to the 10 leading causes. The world's top killers are coronary heart disease and stroke, which accounted for a total of 15 million deaths in 2015. Over the past 15 years, these diseases have remained the leading causes of death in the world. In 2015, chronic obstructive pulmonary disease claimed 3.2 million lives, while lung cancer (together with cancer of the trachea and bronchi) was responsible for 1.7 million deaths.

Chronic vascular disease (CVD) is the number 1 cause of death in the world, with more people dying each year from CVD than from any other cause. An estimated 17.7 million people died from CVD in 2015, accounting for 31% of all deaths worldwide. Of these deaths, approximately 7.4 million were due to coronary heart disease and 6.7 million were due to stroke.

Most cardiovascular diseases can be prevented by implementing nationwide strategies to address behavioral risk factors such as tobacco use, unhealthy diets and obesity, physical inactivity and the harmful use of alcohol.

Cancer is the second leading cause of death in the world and was responsible for 8.8 million deaths in 2015. Worldwide, nearly 1 in 6 deaths are related to cancer. Approximately 70% of cancer deaths occur in low- and middle-income countries. About one third of cancer deaths are attributable to the top 5 behavioral and dietary risks: high body mass index, low fruit and vegetable intake, physical inactivity, tobacco use, and alcohol use.

Additionally, according to the Veterinary Cancer Society, cancer is the leading cause of death in 47% of dogs, especially dogs over ten years old, and 32% of cats. As of January 2015, 39% of dog owners own a dog that is 7 years old or older, while 43% of cat owners own a cat in the oldest age category. These companion animals are at high risk for cancer.

In addition, over 7.5 million children under the age of 5 die every year from malnutrition and mostly preventable diseases.

In this regard, the search continues for the use of new nutritional mixtures that can affect various health conditions in animals, especially those that can improve the quality of life and increase the life span of the animal.

Statement of the Invention

The present disclosure pertains essentially to nutritional formulas that provide a health benefit to an animal. More specifically, the present disclosure relates to methods and compositions that use at least four of the nutrients selected from the group consisting of walnut, vulture curly extract, epigallocatechin gallate (EGCG), turmeric root powder, lycopene, taurine, eicosapentaenoic acid. (EPA) and docosahexaenoic acid (DHA).

Accordingly, in a general embodiment, a method of providing a health benefit to an animal may comprise orally administering to the animal a composition comprising at least four of the nutrients selected from the group consisting of walnut, griffon curly extract, EGCG, turmeric root powder, lycopene, taurine, EPA and DHA.

In addition, in another embodiment, the composition may contain a nutritional mixture, and the nutritional mixture includes at least four nutrients selected from the group consisting of walnut in an amount of from 60.61% to 83.33% of the nutritional mixture, extract grifola curly in the amount of 0.0017% to 0.076% of the nutrient mixture, EGCG in the amount of 0.167% to 1.52% of the nutrient mixture, turmeric root powder in the amount of 16.67% to 24.24% of the nutrient mixture, lycopene in the amount of 0.0167% to 0.033% of formula, taurine 0.833% to 1.52% of formula, EPA 0.833% to 6.06% of formula, and DHA 0.833% to 6.06% nutrient mixture. Such a nutrient mixture can provide an animal with a healthy effect.

Such health benefits may include treating or preventing cancer, providing an anti-inflammatory effect, providing an analgesic effect, treating or preventing arthritis, managing pain, treating or preventing tendinitis, treating or preventing neurodegenerative diseases, treating or preventing inflammatory bowel disease, treating or preventing retinal disease, treating or preventing autoimmune disease, treating or preventing depression, treating or preventing heart disease, treating or preventing kidney or kidney disease, treating or preventing dermatitis, treating or preventing allergic reactions, and treating or preventing liver disease.

Additional features and advantages are described herein and will become apparent upon reading the following detailed description.

Detailed description

Definitions

The following are some definitions. However, definitions may be placed in the "Embodiments" section below, and the above heading "Definitions" does not imply that the descriptions in the "Embodiments" section are not definitions.

As used herein and in the appended claims, the singular forms include references to the plural unless the context clearly dictates otherwise. Thus, for example, a reference to "ingredient" or "specific ingredient" includes two or more ingredients. The term "and/or" used in the context of "X and/or Y" should be interpreted as "X" or "Y" or "X and Y". As used herein, the term "example", especially when followed by a listing of terms, is purely exemplary and illustrative and should not be construed as exclusive or exhaustive.

As used herein, the term "about" should be understood to refer to numbers in a numerical range, such as in the range of -10% to +10% of the specified number, preferably -5% to +5% of the specified number, more preferably from -1% to +1% of said number, most preferably -0.1% to +0.1% of said number. The range "between" two values includes those two values. In addition, it should be understood that all numerical ranges herein include all numbers, integers and fractions, within the range. Moreover, these numerical ranges should be considered as supporting a claim referring to any number or subset of numbers within the given range. For example, the description "from 1 to 10" should be considered as confirming the range "from 1 to 8", "from 3 to 7", "from 1 to 9", "from 3.6 to 4.6", "from 3, 5 to 9.9", etc.

Unless otherwise indicated, all percentages herein are expressed by weight based on the total weight of the composition. When referring to pH, the values correspond to pH measured at 25 °C with standard equipment.

The terms "food", "food product" and "food composition" mean a product or composition that is intended for ingestion by an animal and provides the animal with at least one nutrient. The term "pet food" means any food composition intended for consumption by a dog or cat.

The term "animal" refers to any animal that may benefit from the use of one or more of the methods or compositions of the present invention, including humans, birds, cattle, canines, equines, felines, goats, wolves, mice, and rats. , sheep and pigs.

The term "companion animal" means a dog or cat. As used herein, the terms "dog" and "canine" may be used interchangeably. In one embodiment, the companion animal may be a canine. In another embodiment, the companion animal may be a cat.

"Wet food" means pet food having a moisture content of from about 50% to about 90%, and in one aspect from about 70% to about 90%. "Dry food" means pet food having a moisture content of less than about 20%, and in one aspect, less than about 15%, and in a particular aspect, less than about 10%. "Semi-moist food" means a pet food having a moisture content of from about 20% to about 50%, and in one aspect from about 25% to about 35%. "Pellets" means pieces of dry or semi-moist pet food, which may be in the form of pellets or any other form. Non-limiting examples of granules include particles; pellets; pieces of pet food, dehydrated meat, meat analogue, vegetables, and combinations thereof; and snacks for pets such as jerky or vegetables, rawhide, and biscuits.

The compositions described herein may not contain any element that is not specifically described herein. Thus, a description of an embodiment using the term "comprising" includes a description of embodiments "consisting essentially of" and "consisting of" these components. Similarly, the methods described herein may not include any step that is not specifically described herein. Thus, a description of an embodiment using the term "comprising" includes a description of embodiments "consisting essentially of" and "consisting of" the steps indicated. Moreover, the description of some steps as "optional" does not imply that other steps that are not explicitly described as optional are necessarily required.

Any embodiment described herein may be combined with any other embodiment described herein.

"Prevention" includes reducing the risk and/or severity of a condition or disorder. The terms "treatment", "treat", "curative" and "alleviate" include both prophylactic or preventative treatment (which prevents and/or slows the development of the target condition or disorder) and curative, therapeutic or disease-modifying treatment. , including therapeutic measures that allow to cure, slow down, reduce symptoms and / or stop the progression of the diagnosed pathological condition or disorder; and the treatment of patients at risk of contracting the disease or suspected of contracting the disease, as well as patients who are ill or have been identified as suffering from a disease or medical condition. The term does not necessarily imply that the subject is receiving treatment until complete recovery. The terms "treatment", "treat" and "treatment" also refer to maintaining and/or promoting health in an individual who is not suffering from a disease but may be susceptible to developing a disease state. The terms "treatment", "treat", "treatment" and "alleviate" should also include potentiation or other enhancement of one or more primary preventive or therapeutic measures. The terms "treatment", "treat", "treatment", and "alleviate" are further intended to include dietary therapy for a disease or condition, or dietary therapy for the prevention or prevention of a disease or condition. Treatment can be viewed from the perspective of the patient or the physician.

Relative terms "improved", "increased", "enhanced", etc. refer to the effects of a composition described herein (a composition containing a therapeutically effective amount of any of the nutrients described herein, or a prophylactic dose of any of the nutrients described herein) relative to a composition having a lower content or no any of the nutrients described herein, but otherwise identical.

As used herein, the term "walnut" refers to any type of walnut, including, without limitation, walnut and black walnut, and includes any part or processing thereof, including, but not limited to, whole nut, chopped walnut walnut, walnut flour, etc.

As used herein, the term "grifola curly extract" refers to the mushroom Grifola frondosa and includes any part or any extract thereof.

Embodiments

One aspect of the present disclosure is a method of providing a health benefit to an animal, comprising orally administering to the animal a composition containing at least four of the nutrients selected from the group consisting of walnut, griffola curly extract, EGCG, turmeric root powder, lycopene, taurine, EPA and DHA.

In addition, in another embodiment, the composition may contain a nutritional mixture, and the nutritional mixture includes at least four nutrients selected from the group consisting of walnut in an amount of from 60.61% to 83.33% of the nutritional mixture, extract grifola curly in the amount of 0.0017% to 0.076% of the nutrient mixture, EGCG in the amount of 0.167% to 1.52% of the nutrient mixture, turmeric root powder in the amount of 16.67% to 24.24% of the nutrient mixture, lycopene in the amount of 0.0167% to 0.033% of formula, taurine 0.833% to 1.52% of formula, EPA 0.833% to 6.06% of formula, and DHA 0.833% to 6.06% nutrient mixture. Such a nutrient mixture can provide an animal with a healthy effect.

As such, the methods and compositions include various nutrients that provide a health benefit to the animal. Such nutrients may include walnut, griffon curly extract, EGCG, turmeric root powder, lycopene, taurine, EPA and DHA. In various aspects, the composition may contain at least 4 nutrients, 5 nutrients, 6 nutrients, 7 nutrients or, in one particular aspect, all 8 nutrients. In one aspect, the composition may include at least 4 nutrients, including vulture curly extract, turmeric root powder, taurine, and walnut.

As such, the nutrients may be present in a therapeutically effective amount to provide a health benefit to the animal. Such nutrients may be present at various concentrations depending on the composition. In one embodiment, the composition may be a food. In such an embodiment, the nutrients may be present as follows: walnut may be present in an amount of 5% to 20%, in one aspect, a walnut may be present in an amount of 10% to 15%; Griffola curly extract may be present in an amount of 0.0001% to 0.05%, in one aspect, Griffola curly extract may be present in an amount of 0.0001% to 0.025%; EGCG may be present in an amount of 0.01% to 0.5%, in one aspect EGCG may be present in an amount of 0.05% to 0.1%; turmeric root powder may be present in an amount of 1% to 8%; in one aspect, turmeric root powder may be present in an amount of 3% to 5%; lycopene may be present in an amount of 0.001% to 0.01%; in one aspect, lycopene may be present in an amount of 0.005% to 0.008%; taurine may be present in an amount of 0.05% to 0.5%, in one aspect taurine may be present in an amount of 0.1% to 0.3%; EPA may be present in an amount of 0.05% to 2%, in one aspect EPA may be present in an amount of 0.1% to 1%; and DHA may be present in an amount of 0.05% to 2%, in one aspect DHA may be present in an amount of 0.1% to 1%.

In addition, in another embodiment, the composition may be an additive. In such an embodiment, the nutrients may be present as follows: walnut is present in an amount of 60.61% to 83.33% of the supplement, grifola curly extract is present in an amount of 0.0017% to 0.076% of the supplement, EGCG is present in an amount of 0.167 % to 1.52% of the additive additive, turmeric root powder is present in the amount of 16.67% to 24.24% of the additive, lycopene is present in the amount of 0.0167% to 0.033% of the additive, taurine is present in the amount of 0.833% to 1 .52% additive, EPA present at 0.833% to 6.06% additive, and DHA present at 0.833% to 6.06% additive.

Such combinations of nutrients can provide a health benefit to the animal. In one embodiment, the health benefit can be any of the following: treating or preventing cancer, providing an anti-inflammatory effect, providing an analgesic effect, treating or preventing arthritis, managing pain, treating or preventing tendonitis, treating or preventing neurodegenerative diseases, treating or preventing inflammatory bowel disease, treatment or prevention of retinal disease, treatment or prevention of autoimmune disease, treatment or prevention of depression, treatment or prevention of heart disease, treatment or prevention of kidney or kidney disease, treatment or prevention of dermatitis, treatment or prevention of allergic reactions, and treatment or prevention of liver disease .

True nutritional formulas can be used in combination with any diet or regimen. As noted, the nutrient mixture may be part of the feed or supplement. In one aspect, the food may be pet food. In another aspect, the food may be a complete and nutritionally balanced pet food. In another embodiment, the nutritional formula may be included in a treat, sauce, or other accompanying food component. Real nutritional formulas can be used to achieve health benefits in any animal. In one aspect, the animal may be a companion animal. In another aspect, the companion animal may be a dog. In another aspect, the companion animal may be a cat.

As such, the present nutritional formulas can be administered for a sufficient time to achieve a health benefit in the animal. In one embodiment, the introduction can be done on a regular basis. In some embodiments, nutritional formulas may be administered to a companion animal over a period of at least one week, at least one month, at least two, three, four, five, or six months; and in some embodiments, for at least one year, or even for the lifetime of the animal. During this time period, formulas may be administered to the dog at least one day per week, at least two days per week, at least three, four, five, or six days per week; or even seven days a week. Nutritional mixtures can be administered as a single dose per day or as multiple separate doses per day.

In one embodiment, the nutritional formulas can be administered in an amount that provides from about 0.1 g to 10 g of walnut per kg of animal body weight per day. In one aspect, you can enter from 0.5 g to about 6.5 g of walnut per kg of body weight of the animal per day. In one embodiment, the nutritional formulas can be administered in an amount that provides from about 0.1 mg to 30 mg of vulture curly extract per kg of animal body weight per day. In one aspect, you can enter from 0.15 mg to about 20 mg of griffola curly extract per kg of body weight of the animal per day. In one embodiment, nutritional formulas can be administered in an amount that provides from about 0.5 mg to 200 mg of EGCG per kg of animal body weight per day. In one aspect, you can enter from 1 mg to about 180 mg of EGCG per kg of animal body weight per day. In one embodiment, nutritional formulas can be administered in an amount that provides from about 0.05 g to 5 g of turmeric root powder per kg of animal body weight per day. In one aspect, 0.1 g to about 4 g of turmeric root powder per kg of animal body weight per day can be administered. In one embodiment, the nutritional formulas can be administered in an amount that provides from about 0.005 g to 1 g of lycopene per kg of animal body weight per day. In one aspect, you can enter from 0.01 g to about 0.5 g of lycopene per kg of body weight of the animal per day. In one embodiment, the nutritional formulas can be administered in an amount that provides from about 1 mg to 300 mg of taurine per kg of animal body weight per day. In one aspect, 5 mg to 200 mg of taurine per kg of animal body weight per day can be administered. In one embodiment, nutritional formulas can be administered in an amount that provides from about 1 mg to 1000 mg of EPA per kg of animal body weight per day. In one aspect, you can enter from 5 mg to about 750 mg of EPA per kg of animal body weight per day. In one embodiment, formulas can be administered in an amount that provides from about 1 mg to 1000 mg of DHA per kg of animal body weight per day. In one aspect, you can enter from 5 mg to about 750 mg of DHA per kg of animal body weight per day.

[0037] The pet food compositions described herein can be any food formulated for consumption by a pet, such as a dog. In one embodiment, the pet food composition provides complete nutrition as defined by the Association of American Feed Control Officials (AAFCO) and depending on the type of animal for which the composition is intended (eg, dogs).

The pet food composition may contain meat, such as emulsified meat. Examples of suitable meats include poultry, beef, pork, lamb and fish, especially types of meat suitable for pets. The meat may include any additional parts of the animal, including offal. Some or all of the meats may be offered as one or more meat meals, namely meat that has been dried and ground to a substantially uniform particle size and as defined by AAFCO. In a further or alternative embodiment, a vegetable protein such as pea protein, corn protein (e.g. ground corn or corn gluten), wheat protein (e.g. ground wheat or wheat gluten), soy protein (e.g. soy flour, soy concentrate) may be used. or soy isolate), rice protein (such as milled rice or rice gluten), etc.

The pet food compositions described herein may contain one or more of vegetable oil, flavor, color, or water. Non-limiting examples of suitable vegetable oils include soybean oil, corn oil, cottonseed oil, sunflower oil, canola oil, peanut oil, safflower oil, and the like. In some embodiments, lipids in the composition may include medium chain triglycerides. (MCT) or one or more of any vegetable oil, any fish oil, a lipid from any meat, and any omega-3 fatty acids.

Non-limiting examples of suitable flavors include yeast, tallow, processed animal meal (e.g., poultry, beef, lamb, pork), flavor extracts or blends (e.g., baked beef), animal digests, and the like. Non-limiting examples of suitable colors include food, pharmaceutical, and cosmetic (FD&C) colors such as blue #1, blue #2, green #3, red #3, red #40, yellow #5, yellow No. 6, etc.; natural colors such as caramel color, annatto, chlorophyllin, cochineal, betanine, turmeric, saffron, paprika, lycopene, elderberry juice, pandanus, clitoris, etc.; titanium dioxide; and any suitable food color known to the person skilled in the art.

The pet food composition described herein may optionally include additional ingredients such as starches, humectants, oral care ingredients, preservatives, amino acids, fibers, prebiotics, sugars, animal oils, flavors, other fats as vegetable oil supplements or alternatives, salts, vitamins, minerals, probiotic microorganisms, biologically active molecules, or combinations thereof.

Non-limiting examples of suitable starches include starches from grains such as corn, rice, wheat, barley, oats, potatoes, peas, beans, cassava, and the like, and from a mixture of these grains, and may be included, by at least partially, in any flour. Non-limiting examples of suitable humectants include salt, sugars, propylene glycol, and polyhydric glycols such as glycerol and sorbitol, and the like. Non-limiting examples of suitable oral care ingredients include alfalfa nutritional concentrate containing chlorophyll, sodium bicarbonate, phosphates (e.g., tricalcium phosphate, acid pyrophosphates, sodium tetrapyrophosphate, metaphosphates, and orthophosphates), peppermint, cloves, parsley, ginger, and the like. Non-limiting examples of suitable preservatives include potassium sorbate, sorbic acid , sodium methyl parahydroxybenzoate, calcium propionate, propionic acid, and combinations thereof.

The specific amounts of each additional ingredient in the pet food compositions described herein will depend on a variety of factors, such as the ingredient included in the first food material and any second food material; kind of animal; age, body weight, general health, sex and diet of the animal; consumption rate for the animal; the purpose for which the food is given to the animal, and the like. Thus, the ingredients and their amounts can vary widely.

EXAMPLE

By way of example and without limitation, the following non-limiting study is illustrative of the compositions and methods of using animal formulas in one or more of the embodiments provided herein.

Example 1 - Feeding Study

Female nude mice aged 3–4 weeks (n = 50) were randomly assigned based on basal body weight into 10 groups (n = 4–5/group), which were randomly assigned to one of ten diets with a one-week transition period until the end of the study. from the original diet to the experimental diets. All diets were freely available to mice. Each week, fresh rations were weighed and given to mice three times. After six weeks of feeding the experimental diets, 1 x 10 ⁶ human breast cancer cells of the MDA-MB231 cell line were injected subcutaneously into the right flank of each mouse. The appearance and size of the tumor every two weeks was recorded and measured with a caliper.

The following diets were used in the study. Control: AIN-93G (Research Diets, Inc.) (18% protein, 7.2% fat, 63% carbohydrate, 5% fiber, the rest is moisture). AIN-93G + Nutritional Blend (NB) (replacing 16.46% carbs with 11.3% whole walnut flour, 0.005% curly griffon extract, 0.05% EGCG, 3% turmeric root powder, 0.005% lycopene, 0 ,1% taurine and 2% fish oil) containing 19.9% protein, 9.7% fat, 53.2% carbohydrate, 6.1% fiber and 11.1% moisture. AIN-93G + Turmeric Root Powder (replacing 3% carbs with 3% Turmeric Root Powder) at 18% protein, 7.2% fat, 63% carbs, 5% fiber, and 6.8% moisture. AIN-93G + EGCG (replacing 0.05% carbs with 0.05% EGCG) at 18% protein, 7.2% fat, 63% carbs, 5% fiber, and 6.8% moisture. AIN-93G + EGCG + Turmeric Root Powder (replacing 0.05% carbs with 0.05% EGCG and replacing 3% carbs with 3% turmeric root powder) with 18% protein, 7.2% fat, 63% carbs, 5% fibers and 6.8% moisture. AIN-93G + Lycopene (replacing 0.005% carbs with 0.005% lycopene) with 18% protein, 7.2% fat, 63% carbohydrate, 5% fiber, and 6.8% moisture. AIN-93G + Fish Oil (manufactured by Omega Protein) (replacing 2% carbs with 2% fish oil) with 18% protein, 7.2% fat, 63% carbohydrate, 5% fiber, and 6.8% moisture. AIN-93G + Taurine (replacing 0.1% carbs with 0.1% taurine) is 18% protein, 7.2% fat, 63% carbohydrate, 5% fiber, and 6.8% moisture. AIN-93G + Griffola Curly Extract (manufactured by Aloha Medicinals Inc.) (replacing 0.0005% carbohydrates with 0.0005% Griffola Curly Extract) with 18% Protein, 7.2% Fat, 63% Carbohydrates, 5% Fiber and 6.8% moisture. AIN-93G + Walnut Flour (Oh! Nuts®, available at www.ohnuts.com) (replacing 11.3% carbs with 11.3% walnuts) with 21% protein, 8% fat, 57.5% carbohydrates, 5% fiber and 8.5% moisture. Nutritional formula (NB): 11.3% whole walnut flour, 0.005% curly griffon extract, 0.05% EGCG, 3% turmeric root powder, 0.005% lycopene, 0.1% taurine, and 2% fish oil.

The results of the study show that only the composition "NB" reduced the likelihood of cancer (table 1) and slowed down the growth of cancer (table 2 and 3). Single nutrient supplemented formulations did not provide similar prevention effects. The data clearly show that the NB formulation provided unexpected cancer prevention effects that cannot be achieved with single nutrient formulations. Additionally, the combination of EGCG and "turmeric root powder" also did not show any cancer prevention effects, which refutes the published data on the antitumor effects of EGCG and "turmeric root powder" as reported by Zhou DH, Wang X, Yang M, Shi X, Huang W, Feng Q. “Combination of low concentration of (-)-epigallocatechin gallate (EGCG) and curcumin strongly suppresses the growth of non-small cell lung cancer in vitro and in vivo through causing cell cycle arrest” Int J Mol Sci. 2013;14(6):12023-36. The results presented are completely unexpected as EGCG, fish oil and Griffola curly extract were used in the present NB mixture, which alone resulted in an increase in tumor size compared to the control diet (see Table 2). The present NB composition provided effective treatment for cancer in animals and can be used to prevent or treat animals at high risk of developing cancer, as well as animals that have received anti-cancer treatment.

Table 1

Group Tumor appearance (2 weeks after tumor inoculation) Tumor growth (6 weeks after tumor inoculation) Control Diet 5/5 5/5 Turmeric Root Powder Diet 5/5 5/5 Diet with EGCG 5/5 5/5 EGCG Diet + Turmeric Root Powder 5/5 5/5 Diet with lycopene 5/5 5/5 Diet with fish oil 5/5 5/5 Diet with taurine 4/4 4/4 Diet with mushroom 5/5 5/5 Diet with walnuts 5/5 4/5 Ration with NB 5/5 3/5

table 2

Group Average tumor size (mm ³ ) Control Diet 190.09 Turmeric Root Powder Diet 57.73 Diet with EGCG 186.31 EGCG + Turmeric Root Powder 130.39 Diet with lycopene 124.54 Diet with fish oil 274.14 Diet with taurine 78.27 Diet with mushroom 256.57 Diet with walnuts 175.92 Ration with NB 19.40

Table 3

Group No tumor (0) Reversible stage (< 10 mm ³ ) Stage I (< 25 mm ³ ) Stage II (< 50 mm ³ ) Stage III (< 100 mm ³ ) Stage IV (< 200 mm ³ ) Stage V (< 400 mm ³ ) Stage VI (< 800 mm ³ ) Control diet twenty% 40% 40% Turmeric Root Powder Diet twenty% twenty% 60% Diet with EGCG 40% twenty% twenty% twenty% EGCG Diet + Turmeric Root Powder 40% twenty% twenty% twenty% Diet with fish oil
(source of DHA and EPA) twenty% 40% twenty% twenty% Diet with lycopene 60% twenty% twenty% Diet with vulture curly twenty% twenty% twenty% 40% Diet with taurine 25% 25% fifty% Diet with walnuts twenty% 40% twenty% twenty% Ration with NB 40% 40% twenty%

It should be understood that various changes and modifications to the currently preferred embodiments described herein will be apparent to those skilled in the art. Such changes and modifications may be made without departing from the spirit and scope of the present subject matter and without diminishing its intended benefits. Therefore, the appended claims are intended to cover such changes and modifications.

Claims (11)

1. A method for the treatment or prevention of breast cancer in an animal, comprising oral administration to the animal of a composition containing 11.3 wt.% flour from whole walnuts, 0.005 wt.% extract of curly vulture, 0.05 wt.% epigallocatechin gallate (EGCG) , 3 wt.% turmeric root powder, 0.005 wt.% lycopene, 0.1 wt.% taurine and 2 wt.% fish oil. 2. The method of claim 1, wherein the composition is administered as a feed or supplement. 3. The method according to p. 1, and the composition is an additive. 4. The method according to claim 1, wherein the composition is a food. 5. The method of claim 1, wherein the composition is a complete and nutritionally balanced pet food and the animal is a companion animal. 6. The method according to p. 1, and the introduction is carried out on a regular basis. 7. Composition for the treatment or prevention of breast cancer in an animal, containing 11.3 wt.% whole walnut flour, 0.005 wt.% curly griffon extract, 0.05 wt.% EGCG, 3 wt.% turmeric root powder, 0.005 wt.% lycopene, 0.1 wt.% taurine and 2 wt.% fish oil. 8. Composition according to claim 7, moreover, the formulation of the composition is in the form of an additive or feed. 9. A composition according to claim 7, wherein the composition is a pet food and the animal is a companion animal. 10. Composition according to claim 7, wherein the composition is an additive. 11. Composition according to claim 7, wherein the composition is a food.