RU2520036C2 - "mioactive-sport" food product for alimentation of people experiencing intensive physical strain - Google Patents

Abstract

FIELD: food industry.

SUBSTANCE: invention relates to food industry, namely, to biologically active additives, in particular, to biologically active additives based food products. The food product for alimentation of people experiencing intensive physical strain contains the following components, wt %: cattle brain hydrolysate peptides with molecular weight equal to 0.2 - 10 kDa - 0.5-10.0, milk whey protein - 5.0-30.0, milk whey protein hydrolysates - 5.0-20.0, haemoglobin - 0.5-5.0, dry concentrate of chicken broth - 1.0-5.0, medium-chain triglycerides - 1.0-10.0, linoleic acid - 1.0-10.0, lecithin - 0.5-5.0, amber acid - 0.05-0.5, fumaric acid - 0.025-0.25, inuline - 1.0-10.0, lactitol - 1.0-10.0, stevioside - 0.01-0.1, B vitamins complex - 0.01-0.1, dried food girasol - 1.0-10.0, ascorbic acid - 0.05-0.5, iodised milk proteins - 0.002-0.015, mineral calcium enricher - 0.5-5.0.

EFFECT: invention allows to manufacture a product with high biological and food value.

6 tbl, 6 ex

Description

The invention relates to the food industry, and more specifically to the production and use of biologically active additives, and, in particular, food products based on protein-amino acid modules, and can be used both as specialized additives to functional food products, and as an independent full food product.

A large number of different compositions are known containing biologically active additives (BAA) of a specific effect on the functions (work performed) of individual organs and the body as a whole [1-3].

Such compositions contain dietary supplements aimed at solving particular problems, and, as a rule, they are a food product (carrier), various vitamin, mineral (microelements) additives and dietary supplements proper (both single and complex, especially if they are made, for example, from plant materials), and also, if necessary, a set of stabilizing and preserving components. The main trend characteristic of the compositions under consideration is the maximum use of dietary supplements of natural origin, in particular plant and animal extracts.

Thus, in particular, a protein-peptide module is known, which is a mixture of a protein hydrolyzate of protein-containing wastes from the production of livestock products (amino acids 5-15, peptides no higher than 3000 Da 55-70, polypeptides higher than 3000 Da 15-35) and concentrate / or isolates milk whey proteins, serum / or plasma proteins, blood hemoglobin and concentrates and / or legume protein isolates [4].

The disadvantage of this protein-peptide module, despite the positive indicators of the amino acid scores, is the limited regulatory effect on the body, since the specified module actually carries only one functional load - supplying the body with regulatory proteins and the same set of amino acids.

A complex additive with biologically active properties is also known, including whey proteins and meat protein hydrolysates, blood hemoglobin of farm animals, iodized milk whey proteins, calcium fortifier and dietary fiber [5].

The specified protein-peptide complex nevertheless has low efficiency, since its components, in general, are not significantly involved in the regulation of biochemical processes in the body.

The closest to the claimed food product in terms of composition and technical result is a specialized protein product for the nutrition of athletes and people leading an active lifestyle, containing whey protein concentrate, L-arginine and L-glutamine, a complex of vegetable dietary fiber and gum arabic, as well as vitamin and mineral premixes and flavoring and aromatic additives [6].

The main disadvantage of this protein product is a narrow range of selective effects on the course of various biochemical processes in the body (for example, the body's resistance to high physical exertion under conditions of hypoxia).

The technical result, the achievement of which the creation of this invention is directed, is to increase the biological and nutritional value of the food product and expand the range of its effective effect on the body by introducing dietary supplements that trigger and promote the mechanisms of natural self-regulation of biochemical processes in the body.

The technical result is achieved by the fact that the claimed food product (PP) based on proteins, dietary fiber, vitamin and mineral premixes as proteins includes a mixture of peptides of cattle brain hydrolyzate with a molecular weight of 0.2-10.0 kDa, milk whey protein hydrolysates and milk whey proteins, additionally includes hemoglobin and dry chicken stock concentrate, medium chain triglycerides, linoleic acid, lecithin, succinic and fumaric acids, ascorbic acid, inulin, lactitol and steviosis id, as a vitamin premix - a complex of vitamins B, soluble and insoluble dietary fiber - Jerusalem artichoke dried, iodized milk proteins, and as a mineral premix - biogenic calcium with the following ratio of components (wt.%):

cattle hydrolyzate peptides with a molecular weight of 0.2-10 kDa 0.5-10.0 milk whey proteins 5.0-30.0 whey protein hydrolysates 5.0-20.0 hemoglobin 0.5-5.0 dry chicken stock concentrate 1.0-5.0 medium chain triglycerides 1.0-10.0 linoleic acid 1.0-10.0 lecithin 0.5-5.0 succinic acid 0.05-0.5 fumaric acid 0.025-0.25 inulin 1.0-10.0 lactitol 1.0-10.0 stevioside 0.01-0.1 vitamin B complex 0.01-0.1 dried artichoke 1.0-10.0 vitamin C 0.05-0.5 iodized milk proteins 0.002-0.015 nutrient calcium 0.5-5.0

The inventive PP can be used both as specialized additives to functional food products, and as an independent full-fledged food product, and, accordingly, may additionally include functional additives and carriers, for example, chocolate mass, milk, including dry, various nuts, pastries, confectionery, etc. Given the composition of the claimed food product can be produced in the form of powder, plastic products of any shape, tablets and other solid or fluid material objects.

When developing the composition of the claimed PP, the following functional and technical characteristics of the components used were taken into account:

Whey proteins (Syntrax, USA), whey protein hydrolysates, blood plasma proteins (Belkoton Plasma, Denmark), and low molecular weight (0.2-10.0 kDa) peptides of cattle and pig hydrolyzed brain extract (cattle) enrich diet with full-fledged animal proteins, correct protein metabolism in diseases of the gastrointestinal tract, provide a complete amino acid composition.

Dietary plant fibers, soluble and insoluble Jerusalem artichoke dried (Ryazan open spaces), fibregam (CNI, France), microcellulose (Bioma, Russia) - promote the prevention and treatment of dysbiosis, increase nonspecific resistance of the body, improve metabolism, normalize microbiocenosis Gastrointestinal tract.

Succinic acid (Bicamber, France) - helps to prevent the phenomenon of hypoxia, especially at high physical exertion, and promotes tissue oxygenation. Succinic acid itself normalizes the functioning of the nervous system, in addition, it neutralizes a large number of poisons in the body.

Bioiod (“Bioiod”, Russia) (monovalent J ⁺ iodine built into the tyrosine or histidine molecule) optimizes the main metabolism in the body.

Vitamin B complex - a mixture of B vitamins based on Supradin or Nutrilit preparations in ratios and amounts that correspond to the “Recommendations for the level of consumption of food and biologically active substances” approved by the Federal Service for Supervision of Consumer Rights Protection and Human Well-being - contributes to activation of the course of biochemical processes in the body. Especially with significant physical exertion.

Biogenic calcium (Farm Group, Russia) - normalizes the excitability of the nervous system, improves the functioning of the cardiac and skeletal muscles, provides a normal level of functioning of the adaptive protective systems of the body.

Fumaric acid (GOST R 53045-2008) in combination with lecithin (Taam Teva, Israel) - counteracts stress at the cellular level due to emergency ATP synthesis, helps normalize lipid metabolism, normalizes tissue respiration, promotes the absorption of vitamins and the formation of red blood cells and hemoglobin.

Ascorbic acid (GOST 4815-76) regulates individual phases of protein metabolism, normalizes the level of blood proteins, strengthens the body's defenses, reduces fatigue and increases physical activity.

Medium chain triglycerides (caprylic and capric acids C ₇ H ₁₅ COOH and C ₉ H ₁₉ COOH, respectively) (Cognis, Germany) are irreplaceable sources of energy in the muscles, liver, and brain due to their direct entry into the bloodstream and almost instant metabolism.

Meat hydrolysates (in this case, chicken stock concentrate) are also rich in valuable trace elements: iron, magnesium, phosphorus, zinc. Hemic iron improves blood composition and oxygenation of tissues, especially the brain, which increases efficiency and delays the onset of fatigue. Magnesium is responsible for the normal functioning of nerve cells, helps to cope with fatigue, relieves irritability, improves sleep. Phosphorus strengthens the musculoskeletal system and teeth. Zinc is good for brain function. All this improves the emotional mood and increase the efficiency of the person as a whole.

It should be noted that the components that make up the claimed PP have been tested and approved and recommended for use by the Research Institute of Nutrition RAMS, the Scientific Center for Children's Health RAMS, the Research Institute of Meat Industry of the RAAS and, in addition, they are substances of natural origin (animal and vegetable raw materials) and are not contain synthetic and toxic substances, hormones, carcinogens and other harmful impurities.

Studies have shown that going beyond the stated balanced amounts and ratios of the main active components in the direction of decreasing, or increasing, or excluding them from the formulation leads to a deterioration in the properties of PP or does not improve the expected effect.

The protein-peptide complex proposed for use in the present invention provides a complete amino acid composition that exceeds the FAO / WHO recommended “ideal” amino acid rate for humans, and the biological value of this protein-peptide complex exceeds the amino acid rate of an ideal FAO / WHO protein (see Table 1) .

Table 1 Indicator FAO / WHO norm Claim PP
Qty per 100 g % SP Proteins, g 75 24 32 - lysine 4.1 1.8 44 - methionine + cystine 2.6 0.7 27 - threonine 3 one 33 - leucine 5.3 2.1 40 - isoleucine 3 1,2 40 phenylalanine + tyrosine 4,5 1.4 31 - valine 3.8 1.3 34 - tryptophan 0.75 0.4 53 Fats, g 83 2.0 2,4 Lecithin, g 7.0 1,2 17 Carbohydrates, g 365 17.0 4.7 Lactulose, g 2 2.0 fifty Dietary fiber, g thirty twenty 67 - including insoluble twenty fourteen 70 - including soluble inulin 10 eleven 110 Vitamin C, mg 70 200 286 Calcium content mg 1000 1200 120 The magnesium content, mg 400 400 one hundred Iron content mg 10-14 8 57-80 - including heme iron, mg 2-4 four 50-100 Zinc content, mg fifteen 10 70 The iodine content, mcg 150 200 133

The inventive PP can be prepared by mixing the components with the sequential introduction of dietary supplements (taking into account their solubility, the ability to evenly distribute in the volume of PP, etc.). Depending on the form of release, i.e. as an independent full-fledged food product or as a food additive, PP is produced in the form of tablets, powders, molded products, drinks, milk mixtures, cereals, instant soups and other similar products.

For a better understanding, the invention can be illustrated, but not exhausted, by the following examples of its specific composition and functionality.

Example 1

PP is prepared by sequential mixing of the calculated amounts of components: 1.0 l of drinking water is poured into a 5-liter mixer equipped with a mixer and, with constant stirring, 20.0 g of succinic acid, 8.0 g of fumaric acid, 374.0 g of inulin are successively loaded , 380.0 g of lactitol, 4.0 g of stevioside, 4.0 g of a complex of vitamins B, 20.0 g of ascorbic acid, 0.6 g of iodinated milk proteins, 40.4 g of mineral calcium fortifier, 360.0 g of medium chain triglycerides , 400.0 g of linoleic acid, 200.0 g of lecithin, 140.0 g of peptides of hydrolyzed head m RSA RNC, 960.0 g of the milk serum proteins, 570.0 g of milk whey protein hydrolysates, 123.0 g of hemoglobin, 200.0 g dry chicken broth concentrate and 196.0 g of dried food artichoke. Mixing is carried out until a homogeneous mass. Receive (in terms of dry product) 4000 g of PP containing (g / 100 g): 3.5 peptides of the brain hydrolyzate of cattle; 24.0 milk whey proteins; 14.25 milk whey protein hydrolysates; 3.075 hemoglobin; 5.0 dry chicken stock concentrate; 9.0 medium chain triglycerides; 10.0 linoleic acid; 5.0 lecithin; 0.5 succinic acid; 0.2 fumaric acid; 9.35 inulin; 9.5 lactitol; 0.1 stevioside; 0.1 complex of vitamins B; 4.9 dried Jerusalem artichoke; 0.5 ascorbic acid, 0.015 iodinated milk proteins and 1.01 nutrient calcium.

Examples 2-5

PP is prepared as in Example 1, except that they change the ratio of the components or their composition. The compositions of the PP in Examples 2-5 are presented in Table 2.

Note: Example 5 is a comparative PP, since it does not contain the most active components, and the content of some is outside the stated limits.

Example 6 (comparative prototype).

77.6 g of whey protein concentrate (Protemix), 7.6 g of L-glutamine (VP-Laboratory), 4.5 g of L-arginine (Twinlab), 7.0 g of fructooligosaccharides (Duphalac, Denmark), 1.0 are mixed g of gum arabic (CNI, France), 0.15 g of vitamin B ₁ , 0.15 g of riboflavin and 2.0 mineral calcium fortifier.

Tests of the claimed PP is carried out by the method of biological testing in the laboratory of biomedicine NTsBT RAMS on laboratory outbred white mice. Comparative tests of the effectiveness of the claimed PP, the protein product of the prototype and the usual diet of the aforementioned laboratory mice were conducted. [7]

The body's resistance to the action of maximum muscle loads was studied on the model of forced swimming of experimental animals with a load of 10% of body weight to complete fatigue. The experimental animals were divided into 5 groups of 20 individuals (no gender difference) and fed them the studied food products in accordance with Table 3.

PP was fed daily for 10 days.

Table 3 Group 1 Group 2 Group 3 Group 4 Group 5 PP according to Example 2 (Table 3) PP according to Example 3 (Table 3) PP according to Example 5 (Table 3) PP prototype (Example 6) Regular diet

The results of biological testing are presented in Table 4.

Table 4 The effect of PP on the life span of mice; forced swimming, first swimming / second swimming; seconds Group 1 Group 2 Group 3 Group 4 Group 5 1st day 137 ± 4/238 ± 7 141 ± 5/243 ± 6 112 ± 5/134 ± 5 115 ± 5/135 ± 5 87 ± 3/120 ± 5 2nd day 165 ± 5/259 ± 6 170 ± 5/262 ± 5 118 ± 6/141 ± 7 116 ± 5/140 ± 6 95 ± 6/125 ± 5 3rd day 210 ± 6/269 ± 5 192 ± 5/287 ± 4 114 ± 4/124 ± 5 111 ± 6/133 ± 7 84 ± 7/110 ± 4 4th day 147 ± 4/213 ± 4 132 ± 5/223 ± 7 105 ± 5/120 ± 8 104 ± 6/118 ± 6 68 ± 6/102 ± 8 5th day 123 ± 7/168 ± 7 127 ± 5/155 ± 6 81 ± 5/104 ± 6 81 ± 5/102 ± 4 55 ± 5/90 ± 5

Clinical trials of the claimed PP, with the consent of the ethics committee, were conducted on volunteers (athletes swimmers, divers and cyclists) aged 18 to 30 years - 64 people.

Tested the formulation of PP in Examples 2, 4 and 5 (Table. 3), in comparative Example 6 and normal nutrition. The course dose of PP was 30 g twice a day for 3 months. The change in overall physical performance was recorded after a three-month course of taking the claimed food product under conditions simulating hypoxia. The test results are shown in Table 5.

Table 5 Subject group (8 people each) Physical performance (% of initial) Note: Consumed PP 1. Swimmers (50 m) 109 ± 1 PP according to Example 2 (Table 3) 2. Diving swimmers (50 m) 109 ± 1 PP according to Example 4 (Table 3) 3. Swimmers (50 m) 103 ± 1 PP according to Example 5 (Table 3) 4. Swimmers (50 m) 103 ± 1 PP according to Example 6 (comparative) Cyclists 1 109 ± 1 PP according to Example 2 (Table 3) Cyclists 2 109 ± 1 PP according to Example 4 (Table 3) Cyclists 3 102 ± 1 PP according to Example 5 (Table 3) Cyclists 4 103 ± 1 PP according to Example 6 (comparative)

Table 6 presents the results of a study of the effect of PP on the tolerance of extreme physical exertion by athletes, in particular on the performance of cyclists (“to failure”).

Table 6 Cyclists The effect of PP on the tolerance of extreme physical exertion; The distance traveled on the stationary bike (%). The initial state is 100%. Group 1 (Example 2, Table 3) Group 2 (Example 4, Table 3) Group 3 (Example 5, Table 3) Group 4 (Example 6, Table 3) Group 5 (Normal Nutrition) 4th week 108 ± 2.5 109 ± 2.0 102 ± 2.5 103 ± 2.5 102 ± 2.0 8th week 112 ± 3,5 112 ± 4.0 102 ± 2.0 104 ± 2.0 103 ± 3,5 10th week 116 ± 2.5 116 ± 4,5 103 ± 1.5 105 ± 1,0 104 ± 1.5 12th week 118 ± 4 119 ± 4 104 ± 3.0 106 ± 1,0 104 ± 2.5 15th week 118 ± 1,5 120 ± 4,5 104 ± 2.5 106 ± 2.0 105 ± 2.0

As can be seen from the description, given examples of the effectiveness of the claimed PP in comparison with the PP of the prototype and the PP of a normal diet (in studies on laboratory animals), as well as field tests on volunteers in terms of functional parameters, the claimed PP has high biological activity, which is significantly manifested in increasing the body's resistance high physical exertion both under normal conditions and in conditions of hypoxia.

Sources of information taken into account when preparing the application

1. RU 2377929 C1, MKI A23L 1/30, 2010

2. RU 2378940 C2, MKI A23L 1/30, 2010

3. RU 380984 C1, MKI A23L 1/30, 2010

4. RU 2388350, MKI A23L 1/30, 2010

5. RU 2356246 C2, MKI A23L 1/30, 2009

6. RU 2375923, MKI A23L 1/30, 2010 - prototype

7. International recommendations for biomedical research using animals.

Chronicle of the WHO, 1985, T. 39, No. 3.3-96.

Claims (1)

A food product for the nutrition of people subject to intense physical activity, based on proteins, dietary fiber, vitamin and mineral premixes, characterized in that the protein contains a mixture of low molecular weight (0.2-10.0 kDa) cattle brain hydrolyzate peptides, hydrolysates milk whey proteins and milk whey proteins, additionally includes hemoglobin and dry chicken stock concentrate, medium chain triglycerides, linoleic acid, lecithin, succinic and fumaric acids, ascorbic acid, inulin, varnish um and stevioside, vitamin B complex (B _2, B ₆ and B _12), soluble and insoluble dietary fiber - Dried Sweet food, dairy iodinated proteins and calcium mineral dressing, with the following component ratio (wt.%):
cattle hydrolyzate peptides with a molecular weight of from 0.2 to 10 kDa 0.5-10.0 milk whey proteins 5.0-30.0 whey protein hydrolysates 5.0-20.0 hemoglobin 0.5-5.0 dry chicken stock concentrate 1.0-5.0 medium chain triglycerides 1.0-10.0 linoleic acid 1.0-10.0 lecithin 0.5-5.0 succinic acid 0.05-0.5 fumaric acid 0.025-0.25 inulin 1.0-10.0 lactitol 1.0-10.0 stevioside 0.01-0.1 vitamin B complex 0.01-0.1 dried artichoke 1.0-10.0 vitamin C 0.05-0.5 iodized milk proteins 0.002-0.015 mineral calcium fortifier 0.5-5.0